Query: siRNA

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MiR-126 induces myeloma cell line Karpas707 apoptosis by downregulating anti-apoptotic protein MCL.
CONCLUSIONS: MiR-126 induces Karpas707 cell apoptosis by downregulating anti-apoptotic protein MCL, which provides a theoretical basis for the target selection of myeloma. PMID: 30402852 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

HIF-1 α restricts proliferation and apoptosis of Tca8113 cells through up regulation of Hippo signaling pathway under hypoxic conditions.
CONCLUSIONS: Under hypoxic conditions, HIF-1α inhibits the proliferation and apoptosis of Tca8113 cells via the elevation of the Hippo signaling pathway. PMID: 30402847 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Effect of hypoxia on the Twist1 in EMT of cervical cancer cells.
CONCLUSIONS: Hypoxia may account for the increased invasion of HeLa cells, which is realized by the up-regulated GLI-1. PMID: 30402835 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

The involvement of miR-155 in blood pressure regulation in pregnant hypertension rat via targeting FOXO3a.
CONCLUSIONS: The inhibition of miR-155 improves the damage of pregnant hypertension via the upregulation of FOXO3a, which provides academic leads for the future therapy of pregnant hypertension. PMID: 30402830 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Kinome-Wide siRNA Screening Identifies Src-Enhanced Resistance of Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells
Yen-Dun Tony Tzeng, Pei-Feng Liu, Ju-Yueh Li, Li-Feng Liu, Soong-Yu Kuo, Chiao-Wei Hsieh, Cheng-Hsin Lee, Chih-Hsuan Wu, Michael Hsiao, Hong-Tai Chang, Chih-Wen Shu
Source: Frontiers in Pharmacology - November 9, 2018 Category: Drugs & Pharmacology Source Type: research

Uterine RGS2 expression is regulated by exogenous estrogen and progesterone in ovariectomized mice, and down ‐regulation of RGS2 expression in artificial decidualized ESCs inhibits trophoblast spreading in vitro
This article is protected by copyright. All rights reserved.
Source: Molecular Reproduction and Development - November 9, 2018 Category: Reproduction Medicine Authors: Manxi Jiang, Liangshan Hu, Baoping Wang, Danxia Chen, Yahong Li, Zhen Zhang, Yan Zhu Tags: RESEARCH ARTICLE Source Type: research

β‑glucan, a dectin‑1 ligand, promotes macrophage M1 polarization via NF‑κB/autophagy pathway.
Abstract Pro‑inflammatory (M1) macrophages have key roles in atherogenesis. As β‑glucan has been demonstrated to exert pro‑inflammatory effects, the present study examined whether β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype. The results from reverse transcription‑quantitative polymerase chain reaction, flow cytometry, western blotting and transmission electron microscope indicated that M1 macrophage markers inducible nitric oxide synthase and cluster of differentiation 80 were upregulated, dectin‑1 (a receptor for β‑glucan) expression and nuclear factor (NF)...
Source: International Journal of Oncology - November 9, 2018 Category: Cancer & Oncology Authors: Li X, Luo H, Ye Y, Chen X, Zou Y, Duan J, Xiang D Tags: Int J Oncol Source Type: research

ROS-induced HSP70 promotes cytoplasmic translocation of high-mobility group box 1b and stimulates antiviral autophagy in grass carp kidney cells Cell Biology
In this study, we found that both grass carp reovirus (GCRV) infection and hydrogen peroxide (H2O2) treatment induced the accumulation of reactive oxygen species (ROS) in C. idella kidney cells and stimulate autophagy. Suppressing ROS accumulation with N-acetyl-l-cysteine significantly inhibited GCRV-induced autophagy activation and enhanced GCRV replication. Although ROS-induced autophagy, in turn, restricted GCRV replication, further investigation revealed that the multifunctional cellular protein high-mobility group box 1b (HMGB1b) serves as a heat shock protein 70 (HSP70)–dependent, pro-autophagic protein in grass ca...
Source: Journal of Biological Chemistry - November 9, 2018 Category: Chemistry Authors: Youliang Rao, Quanyuan Wan, Hang Su, Xun Xiao, Zhiwei Liao, Jianfei Ji, Chunrong Yang, Li Lin, Jianguo Su Tags: Immunology Source Type: research

Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF- β Signaling Pathway.
Conclusion: By activation of TGF-β signaling, BSHX formula can promote articular cartilage repair by accelerating chondrocyte proliferation and maintaining chondrocyte phenotype, upregulate ECM accumulation, and inhibit matrix degradation. PMID: 30402118 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - November 8, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

γ‐secretase inhibitor DAPT mitigates cisplatin‐induced acute kidney injury by suppressing Notch1 signaling
AbstractOrgan toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin ‐induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti‐cancer effect of cisplatin by Notch inhibiti on comes at the cost of increased kidney injury is unclear. We show here that treatment of mice with cisplatin resulted in a significant increase in Notch ligand D...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Hitesh Soni, Anberitha T. Matthews, Sandeep Pallikkuth, Rajashekhar Gangaraju, Adebowale Adebiyi Tags: ORIGINAL ARTICLE Source Type: research

The roles of hypoxia-inducible Factor-1 and iron regulatory protein 1 in iron uptake induced by acute hypoxia.
CONCLUSIONS: We propose that HIF-1/HRE system might play a principal part in hypoxia induced iron uptake. PMID: 30415773 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - November 8, 2018 Category: Biochemistry Authors: Yang L, Wang D, Wang XT, Lu YP, Zhu L Tags: Biochem Biophys Res Commun Source Type: research

γ-secretase inhibitor DAPT mitigates cisplatin-induced acute kidney injury by suppressing Notch1 signaling.
Abstract Organ toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin-induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti-cancer effect of cisplatin by Notch inhibition comes at the cost of increased kidney injury is unclear. We show here that treatment of mice with cisplatin resulted in a significant increase in Notch liga...
Source: J Cell Mol Med - November 8, 2018 Category: Molecular Biology Authors: Soni H, Matthews AT, Pallikkuth S, Gangaraju R, Adebiyi A Tags: J Cell Mol Med Source Type: research

Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP
Publication date: 6 November 2018Source: Cell Reports, Volume 25, Issue 6Author(s): Roberto Moreno-Vicente, Dácil María Pavón, Inés Martín-Padura, Mauro Català-Montoro, Alberto Díez-Sánchez, Antonio Quílez-Álvarez, Juan Antonio López, Miguel Sánchez-Álvarez, Jesús Vázquez, Raffaele Strippoli, Miguel A. del PozoSummaryThe transcriptional regulator YAP orchestrates many cellular functions, including tissue homeostasis, organ growth control, and tumorigenesis. Mechanical stimuli are a key input to YAP activity, but the mechanisms controlling this regulation remain largely uncharacterized. We show that CAV1 posi...
Source: Cell Reports - November 7, 2018 Category: Cytology Source Type: research

Targeting Thioredoxin System with an Organosulfur Compound, Diallyl Trisulfide (DATS), Attenuates Progression and Metastasis of Triple-Negative Breast Cancer (TNBC)
Conclusion: Collectively, this present study indicates that targeting Trx system with DATS may provide a promising strategy for treating metastasis of TNBC.Cell Physiol Biochem 2018;50:1945 –1963
Source: Cellular Physiology and Biochemistry - November 7, 2018 Category: Cytology Source Type: research

PGC-1 α Participates in the Protective Effect of Chronic Intermittent Hypobaric Hypoxia on Cardiomyocytes
Conclusion: CIHH treatment could reduce the calcium overload and H/R injury in cardiomyocytes by up-regulating the expression of PGC-1 α and regulating the energy metabolism of glucose and lipid. The HIF-1α-AMPK signaling pathway might be involved in the process.Cell Physiol Biochem 2018;50:1891 –1902
Source: Cellular Physiology and Biochemistry - November 7, 2018 Category: Cytology Source Type: research

Expression of the CD59 Glycoprotein Precursor is Upregulated in an Estrogen Receptor-alpha (ER- α)-Negative and a Tamoxifen-Resistant Breast Cancer Cell Line In Vitro.
CONCLUSIONS In summary, our results proposed a candidate biomarker for predicting TAM resistance in ERa-positive breast cancer via targeting CD59, therefore it could be a novel therapeutic option. PMID: 30391994 [PubMed - in process]
Source: Medical Science Monitor - November 7, 2018 Category: Research Tags: Med Sci Monit Source Type: research

GSE114773 small RNA profiling of breast cancer cell lines knockdowns
Contributors : L Fish ; S Zhang ; H GoodarziSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensWe performed small RNA sequencing in breast cancer cells treated with siRNA for Control, DCGR8, DROSHA, TARBP2, DICER
Source: GEO: Gene Expression Omnibus - November 7, 2018 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research

GSE106617 Genome wide expression change by RNF168 knocking down in MCF-7 cells
Contributor : Jian ZhuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe aim to the investigate the role of RNF168 in breast cancer progression. MCF-7 cells were used as the model and RNF168 was silenced by siRNA.
Source: GEO: Gene Expression Omnibus - November 7, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Cyclin D1 silencing impairs DNA double strand break repair, sensitizes BRCA1 wildtype ovarian cancer cells to olaparib.
CONCLUSIONS: Silencing of cyclin D1 combined with olaparib may lead to substantial benefit for ovarian cancer management by mimicking a BRCAness phenotype, and induction of G0/G1 cell cycle arrest. PMID: 30414739 [PubMed - as supplied by publisher]
Source: Gynecologic Oncology - November 7, 2018 Category: Cancer & Oncology Authors: Zhong Q, Hu Z, Li Q, Yi T, Li J, Yang H Tags: Gynecol Oncol Source Type: research

Altered expression of the core circadian clock component PERIOD2 contributes to osteoarthritis-like changes in chondrocyte activity.
Abstract In osteoarthritis, chondrocytes undergo a phenotype shift characterised by reduced expression of SOX9 (sry-box 9) and increased production of cartilage-degrading enzymes, e.g. MMP13 (matrix metalloproteinase 13) and ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5). The chondrocyte clock is also altered. Specifically, the peak level of PER2 is elevated, but peak level of BMAL1 reduced in osteoarthritic chondrocytes. The purpose of this study was to determine whether increased PER2 expression causes disease-associated changes in chondrocyte activity and to identify whether known ri...
Source: Chronobiology International - November 7, 2018 Category: Biology Authors: Rong J, Zhu M, Munro J, Cornish J, McCarthy GM, Dalbeth N, Poulsen RC Tags: Chronobiol Int Source Type: research

MicroRNA-143 inhibits proliferation and promotes apoptosis of thymocyte by targeting CXCL13 in myasthenia gravis mice models.
Abstract Myasthenia gravis (MG) is an autoimmune neuromuscular disorder, affecting the quality of life of millions of people worldwide. The current study aims to determine the relationship between microRNA-143 (miR-143) and CXCL13, and whether it influences the pathogenesis of myasthenia gravis (MG). Thymus specimens were resected from patients with thymic hyperplasia combined MG, and then infused into normal mouse cavities to establish MG mice models. Immunohistochemistry, RT-qPCR, in situ hybridization detection, and Western blot analysis were employed to identify the expression of miR-143 and CXCL13 in MG and n...
Source: Am J Physiol Cell Ph... - November 7, 2018 Category: Cytology Authors: Wu DM, Wen X, Han XR, Wang S, Wang YJ, Shen M, Fan SH, Zhuang J, Zhang ZF, Shan Q, Li MQ, Hu B, Sun CH, Lu J, Zheng YL Tags: Am J Physiol Cell Physiol Source Type: research

ERp57 ‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.
ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel. Int J Oncol. 2018 Nov 07;: Authors: Li S, Zhao X, Chang S, Li Y, Guo M, Guan Y Abstract ERp57 has been identified to be associated with the chemoresistance of human ovarian cancer. However, its biological roles in the chemoresistance phenotype remain unclear. In the present study, the association of ERp57 with paclitaxel‑resistant cellular behavior was investigated and the sensitivity enhancement of chemoresistant human ovarian cancer cells to paclitaxel was examined usin...
Source: International Journal of Oncology - November 7, 2018 Category: Cancer & Oncology Authors: Li S, Zhao X, Chang S, Li Y, Guo M, Guan Y Tags: Int J Oncol Source Type: research

Retrospective and perspective of plant epigenetics in China
Publication date: Available online 6 November 2018Source: Journal of Genetics and GenomicsAuthor(s): Cheng-Guo Duan, Jian-Kang Zhu, Xiao-Feng CaoAbstractEpigenetics refer to the study of heritable changes in gene function that do not involve changes in the DNA sequence. Such effects on cellular and physiological phenotypic traits may result from external or environmental factors or be part of normal developmental program. In eukaryotes, DNA wraps on a histone octamer (two copies of H2A, H2B, H3 and H4) to form nucleosome, the fundamental unit of chromatin. The structure of chromatin is subjected to a dynamic regulation thr...
Source: Journal of Genetics and Genomics - November 6, 2018 Category: Genetics & Stem Cells Source Type: research

MiR-216b inhibits pancreatic cancer cell progression and promotes apoptosis by down-regulating KRAS.
Conclusions: MiR-216b might inhibit pancreatic cancer cell progression and stimulate apoptosis by silencing KRAS. PMID: 30393486 [PubMed]
Source: Archives of Medical Science - November 6, 2018 Category: Biomedical Science Tags: Arch Med Sci Source Type: research

Effects of EIF3B gene downregulation on apoptosis and proliferation of human ovarian cancer SKOV3 and HO-8910 cells
Publication date: January 2019Source: Biomedicine & Pharmacotherapy, Volume 109Author(s): Lei Wang, Ling OuyangAbstractIn order to investigate the role of EIF3B (eukaryotic translation initiation factor 3B, EIF3B) gene in the proliferation and apoptosis of ovarian cancer cells, a lentiviral vector system and shEIF3B lentiviral vector were constructed to transfect human ovarian cancer cells. SKOV3 and HO-8910 cells were used in this experiment. The cell growth was detected by Celigo cell counting assay, the apoptosis rate was measured by flow cytometry and the cell proliferation ability of lentivirus transfected cells was t...
Source: Biomedicine and Pharmacotherapy - November 6, 2018 Category: Drugs & Pharmacology Source Type: research

GSE122124 Gene expression profiling of Huh-7 cells tranfected NC or DEPDC1 siRNA
Contributors : Wubin Guo ; Ziwei WangSeries Type : Expression profiling by arrayOrganism : Homo sapiensMany reports shows that DEPDC1 is up-regulated in many tumor types and promotes cell proliferation and invasion, thereby functioning as an oncogene.Hepatocellular Carcinoma has been shown to overexpress DEPDC1, and up-regulated DEPDC1 associates with poorer prognosis.In addition, we found that DEPDC1 knockdown significantly inhibited HCC cell proliferation, colony formation and migration. In order to investigate the mechanism of DEPDC1 in regulating HCC progression, we performed gene array analysis.
Source: GEO: Gene Expression Omnibus - November 6, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Overexpressed LRIG3 gene ameliorates prostate cancer through suppression of cell invasion and migration.
In conclusion, these data of the current study indicated that overexpressed LRIG3 gene expression might inhibit the viability, adhesion, invasion and migration and promote the apoptosis of PC cells. rAd-mediated LRIG3 may facilitate a novel aspect of the treatment of patients suffering from with PC. PMID: 30412760 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - November 6, 2018 Category: Biochemistry Authors: Chen Y, Wang Q, Wang M, Li M Tags: Int J Biol Macromol Source Type: research

Loss of the Chr16p11.2 ASD candidate gene QPRT leads to aberrant neuronal differentiation in the SH-SY5Y neuronal cell model
ConclusionsIn this study,QPRT was causally related to in vitro neuronal differentiation of SH-SY5Y cells and affected the regulation of genes and gene networks previously implicated in ASD. Thus, our data suggest thatQPRT may play an important role in the pathogenesis of ASD in Chr16p11.2 deletion carriers.
Source: Molecular Autism - November 6, 2018 Category: Molecular Biology Source Type: research

Genome-wide profiling of histone H3K27 acetylation featured fatty acid signalling in pancreatic beta cells in diet-induced obesity in mice
Conclusions/interpretationThese results suggest novel roles for DNA-binding proteins and fatty acid signalling in obesity-induced epigenomic regulation of beta cell function.Data availabilityThe next-generation sequencing data in the present study were deposited at ArrayExpress.RNA-Seq:Dataset name: ERR2538129 (Control), ERR2538130 (Diet-induced obesity)Repository name and number: E-MTAB-6718 - RNA-Seq of pancreatic islets derived from mice fed a long-term high-fat diet against chow-fed controls.ChIP-Seq:Dataset name: ERR2538131 (Control), ERR2538132 (Diet-induced obesity)Repository name and number: E-MTAB-6719 - H3K27ac C...
Source: Diabetologia - November 5, 2018 Category: Endocrinology Source Type: research

Molecules, Vol. 23, Pages 2884: Identification and Characterization of NTB451 as a Potential Inhibitor of Necroptosis
Tae-Bong Kang Necroptosis, or caspase-independent programmed cell death, is known to be involved in various pathological conditions, such as ischemia/reperfusion injury, myocardial infarction, atherosclerosis, and inflammatory bowel diseases. Although several inhibitors of necroptosis have been identified, none of them are currently in clinical use. In the present study, we identified a new compound, 4-({[5-(4-aminophenyl)-4-ethyl-4H-1,2,4-triazol-3-yl]sulfanyl}methyl)-N-(1,3-thiazol-2-yl) benzamide (NTB451), with significant inhibitory activity on the necroptosis induced by various triggers, such as tumor necrosis fac...
Source: Molecules - November 5, 2018 Category: Chemistry Authors: Eun-Jung In Yuno Lee Sushruta Koppula Tae-Yeon Kim Jun-Hyuk Han Kwang-Ho Lee Tae-Bong Kang Tags: Article Source Type: research

Phorbol esters induce PLVAP expression via VEGF and additional secreted molecules in MEK1-dependent and p38, JNK and PI3K/Akt-independent manner.
In conclusion, we show that VEGF-A along with other secreted proteins act synergistically to up-regulate PLVAP in MEK1/Erk1/2 dependent manner, bringing us one step further into understanding the genesis of the essential structures that are endothelial diaphragms. PMID: 30394679 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - November 5, 2018 Category: Molecular Biology Authors: Hamilton BJ, Tse D, Stan RV Tags: J Cell Mol Med Source Type: research

A Novel Regulatory Role of Apoptosis Antagonizing Transcription Factor in the Pathogenesis of NAFLD and HCC
Conclusion AATF drives NAFLD and hepatocarcinogenesis, offering a potential target for therapeutic intervention.This article is protected by copyright. All rights reserved.
Source: Hepatology - November 5, 2018 Category: Internal Medicine Authors: Divya P. Kumar, Prasanna K. Santhekadur, Mulugeta Seneshaw, Faridoddin Mirshahi, Cora Uram Tuculescu, Arun J. Sanyal Tags: Original Article Source Type: research

Molecular characterization of p38 MAPK from blunt snout bream (Megalobrama amblycephala) and its expression after ammonia stress, and lipopolysaccharide and bacterial challenge
Publication date: January 2019Source: Fish & Shellfish Immunology, Volume 84Author(s): Chun-Nuan Zhang, Samad Rahimnejad, Kang-Le Lu, Wen-Hao Zhou, Ji-Liang ZhangAbstractp38 mitogen-activated protein kinase (MAPK) is an important protein which plays a key role in regulating the innate immunity, so exploring its molecular characterization is helpful in understanding the resistance against microbial infections in cultured fish. Here, a full-length cDNA of p38 MAPK was cloned from liver of blunt snout bream (Megalobrama amblycephala) which covered 2419 bp with an open reading frame of 1086 bp encoding 361 amino acids. p38 MAP...
Source: Fish and Shellfish Immunology - November 4, 2018 Category: Biology Source Type: research

Baicalein stimulates fibroblast growth factor 21 expression by up-regulating retinoic acid receptor-related orphan receptor α in C2C12 myotubes
Publication date: January 2019Source: Biomedicine & Pharmacotherapy, Volume 109Author(s): Takao Hirai, Kohei Nomura, Rie Ikai, Ken-ichi Nakashima, Makoto InoueAbstractRetinoic acid receptor-related orphan receptor (ROR) α has been implicated in various physiological functions, including the immune system, inflammation, and circadian rhythms. In the present study, the synthetic RORα/γ agonist SR1078 stimulated the production and gene expression of fibroblast growth factor 21 (FGF21) in C2C12 myotubes. FGF21, a member of the FGF family, plays important roles in the regulation of peripheral glucose tolerance and lipid meta...
Source: Biomedicine and Pharmacotherapy - November 4, 2018 Category: Drugs & Pharmacology Source Type: research

Inhibition of mTORC2/RICTOR Impairs Melanoma Hepatic Metastasis.
In conclusion, these results provide first evidence for a critical role of mTORC2/RICTOR in melanoma liver metastasis via cancer cell/HSC interactions. PMID: 30404068 [PubMed - as supplied by publisher]
Source: Neoplasia - November 4, 2018 Category: Cancer & Oncology Authors: Schmidt KM, Dietrich P, Hackl C, Guenzle J, Bronsert P, Wagner C, Fichtner-Feigl S, Schlitt HJ, Geissler EK, Hellerbrand C, Lang SA Tags: Neoplasia Source Type: research

FEAT enhances INSL3 expression in testicular Leydig cells
FEAT(METTL13) is expressed in fetal and adult Leydig cells in the testis. FEAT ‐silenced MA‐10 Leydig cells showed diminished INSL3 protein. A maleMettl13+/ − mouse exhibited bilateral intraabdominal cryptorchidism and markedly decreased INSL3 expression in Leydig cells. AbstractFEAT, the protein encoded by methyltransferase ‐like 13 (METTL13), is aberrantly upregulated in most human cancers and potently drives tumorigenesis in vivo; however, its role in normal tissues remains elusive. Immunoblotting has displayed weak FEAT expression in normal human tissues, including the testis. Here, we found that FEAT is expres...
Source: Genes to Cells - November 4, 2018 Category: Genetics & Stem Cells Authors: Yan Li, Kyosuke Kobayashi, Kosho Murayama, Kohichi Kawahara, Yuichi Shima, Akira Suzuki, Kenzaburo Tani, Atsushi Takahashi Tags: ORIGINAL ARTICLE Source Type: research

Importance of PLC-Dependent PI3K/AKT and AMPK Signaling in RANTES/CCR5 Mediated Macrophage Chemotaxis.
Authors: Chien HC, Chan PC, Tu CC, Day YJ, Hung LM, Juan CC, Tian YF, Hsieh PS Abstract Regulated upon activation, normal T cell expressed, and secreted (RANTES), also known as chemokine ligand 5 (CCL5), has been reported to facilitate macrophage migration, which plays a crucial role in tissue inflammation. The aim of this study is to investigate the characteristics and underlying mechanism of RANTES on macrophage chemotaxis under physiological and pathological conditions. The study was conducted on macrophage RAW264.7 cell and bone marrow-derived macrophages (BMDM) isolated from CCL receptor 5 (CCR5) knockout mice...
Source: The Chinese Journal of Physiology - November 3, 2018 Category: Physiology Tags: Chin J Physiol Source Type: research

Long noncoding RNA TSLNC8 suppresses cell proliferation and metastasis and promotes cell apoptosis in human glioma.
Authors: Chen D, Yu X Abstract Glioma is among the most common primary brain tumors and one of the most aggressive and lethal forms of human cancer. Long noncoding RNAs (lncRNAs) have demonstrated great importance in the development and progression of cancer. The present study aimed to investigate the role of the novel tumor suppressive lncRNA on Chromosome 8p12 (TSLNC8), in cell proliferation, metastasis and apoptosis in human glioma. It was initially reported that the relative transcript levels of TSLNC8 were significantly decreased in human glioma tissues and cultured glioma cells, as evidenced by RT‑qPCR. Amo...
Source: Molecular Medicine Reports - November 3, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Regulation between two alternative splicing isoforms ZNF148FL and ZNF148ΔN, and their roles in the apoptosis and invasion of colorectal cancer
ConclusionZNF148FL could increase proliferation, invasion, and migration of CRC cells, while ZNF148ΔN showed opposite effect; the two splicing isoforms of ZNF148 may exert a mutual antagonistic effect to each other on the malignant biological activities.
Source: Pathology Research and Practice - November 3, 2018 Category: Pathology Source Type: research

Farrerol attenuates β-amyloid-induced oxidative stress and inflammation through Nrf2/Keap1 pathway in a microglia cell line
In conclusion, farrerol attenuated Aβ-induced oxidative stress and inflammation in BV-2 cells through enhancing the activation of Nrf2/Keap1 pathway. The findings indicated that farrerol could be considered as a therapeutic approach for the treatment of Alzheimer’s disease (AD).
Source: Biomedicine and Pharmacotherapy - November 3, 2018 Category: Drugs & Pharmacology Source Type: research

Nod1-mediated lipolysis promotes diacylglycerol accumulation and successive inflammation via PKCδ-IRAK axis in adipocytes
Publication date: Available online 2 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseAuthor(s): Aditya Sharma, Chandan K. Maurya, Deepti Arha, Amit K. Rai, Sushmita Singh, Salil Varshney, Jonathan D. Schertzer, Akhilesh K. TamrakarAbstractChronic inflammation contributes to obesity mediated metabolic disturbances, including insulin resistance. Obesity is associated with altered microbial load in metabolic tissues that can contribute to metabolic inflammation. Different bacterial components such as, LPS, peptidoglycans have been shown to underpin metabolic disturbances through interactio...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - November 3, 2018 Category: Molecular Biology Source Type: research

Mini Review: Revisiting Mobile RNA Silencing In Plants
This article revisits our present understanding of intercellular and systemic non-cell autonomous RNA silencing, and summarises current debates on RNA signals for mobile silencing. In particular, we discuss new evidence on siRNA mobility, a DCL2-dependent genetic network for mobile silencing and its potential biological relevance as well as 22 nt siRNA being a mobile signal for non-cell-autonomous silencing in both Arabidopsis and Nicotiana benthamiana. This sets up a new trend in unravelling genetic components and small RNA signal molecules for mobile silencing in (across) plants and other organisms of different kingdom...
Source: Plant Science - November 3, 2018 Category: Biology Source Type: research

Dissociation of globotriaosylceramide and impaired endothelial function in α-galactosidase-A deficient EA.hy926 cells
Publication date: Available online 2 November 2018Source: Molecular Genetics and MetabolismAuthor(s): Nayiri Kaissarian, Justin Kang, Liming Shu, Maria J. Ferraz, Johannes Aerts, James A. ShaymanAbstractFabry disease, a rare, X-linked lysosomal storage disease, arises from deficiency of the lysosomal hydrolase, α-galactosidase A (GLA) which disrupts the catabolism of globo- series glycosphingolipids (GSLs). One potential link between GLA deficiency and vascular dysfunction may be changes in endothelial nitric oxide synthase (eNOS) function. GLA-deficient EA.hy926 cells were obtained by siRNA knockdown of GLA expression an...
Source: Molecular Genetics and Metabolism - November 3, 2018 Category: Genetics & Stem Cells Source Type: research

Interleukin-22 Promotes Osteosarcoma Cell Proliferation and Invasion via STAT3 Activation.
CONCLUSIONS These results suggest that IL-22 promotes OS cells proliferation and invasion and its effect is mediated by activation of the STAT3 pathway. These findings demonstrate that IL-22 may serve as a promising molecular biomarker for diagnosis and therapy for OS patients. PMID: 30381753 [PubMed - in process]
Source: Medical Science Monitor - November 3, 2018 Category: Research Tags: Med Sci Monit Source Type: research

FOXRED1 silencing in mice: a possible animal model for Leigh syndrome
AbstractLeigh syndrome (LS) is one of the most puzzling mitochondrial disorders, which is also known as subacute necrotizing encephalopathy. It has an incidence of 1 in 77,000 live births worldwide with poor prognosis. Currently, there is a poor understanding of the underlying pathophysiological mechanisms of the disease without any available effective treatment. Hence, the inevitability for developing suitable animal and cellular models needed for the development of successful new therapeutic modalities. In this short report, we blocked FOXRED1 gene with small interfering RNA (siRNA) using C57bl/6 mice. Results showed neu...
Source: Metabolic Brain Disease - November 3, 2018 Category: Neurology Source Type: research

Baicalein stimulates fibroblast growth factor 21 expression by up-regulating retinoic acid receptor-related orphan receptor α in C2C12 myotubes.
Abstract Retinoic acid receptor-related orphan receptor (ROR) α has been implicated in various physiological functions, including the immune system, inflammation, and circadian rhythms. In the present study, the synthetic RORα/γ agonist SR1078 stimulated the production and gene expression of fibroblast growth factor 21 (FGF21) in C2C12 myotubes. FGF21, a member of the FGF family, plays important roles in the regulation of peripheral glucose tolerance and lipid metabolism and improves metabolic health. The mRNA expression and secretion of FGF21 was significantly weaker in Rora-silenced cells than in cells transf...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - November 3, 2018 Category: Drugs & Pharmacology Authors: Hirai T, Nomura K, Ikai R, Nakashima KI, Inoue M Tags: Biomed Pharmacother Source Type: research

Identification of M Φ specific POTEE expression: Its role in mTORC2 activation via protein-protein interaction in TAMs.
Identification of MΦ specific POTEE expression: Its role in mTORC2 activation via protein-protein interaction in TAMs. Cell Immunol. 2018 Nov 03;: Authors: Vekariya U, Rawat K, Saxena R, Tripathi RK Abstract POTE is known as cancer antigen, expressed in many cancers, along with very few normal tissues like prostate, ovary, testes and embryo. Till date, POTEE identified as majorly expressed POTE paralog. Functionally, POTEF regulates TLR signaling which play important role in innate immunity provided clue about expression of POTE in immune cells. We have chosen three Thp1monocytes, Jurkat T1 and MΦ c...
Source: Cellular Immunology - November 3, 2018 Category: Allergy & Immunology Authors: Vekariya U, Rawat K, Saxena R, Tripathi RK Tags: Cell Immunol Source Type: research

Shikonin Suppresses Lymphangiogenesis via NF- κB/HIF-1α Axis Inhibition.
In conclusion, shikonin inhibits lymphangiogenesis in vitro by interfering the NF-κB/HIF-1α pathway and involves in suppression of VEGF-C and VEGFR-3 mRNA expression. PMID: 30381665 [PubMed - in process]
Source: Biological and Pharmaceutical Bulletin - November 2, 2018 Category: Drugs & Pharmacology Authors: Prangsaengtong O, Jantaree P, Lirdprapamongkol K, Svasti J, Koizumi K Tags: Biol Pharm Bull Source Type: research

Dorsal root ganglia coactivator-associated arginine methyltransferase 1 contributes to peripheral nerve injury-induced pain hypersensitivities
We report here that peripheral nerve injury induced the upregulation of the mRNA and protein expression of CARM1 in the injured DRG, and blocking its expression through small interfering RNA (siRNA) in the injured DRG attenuated the development and maintenance of neuropathic pain. Furthermore, pharmacological inhibition of CARM1 mitigated peripheral nerve injury-induced mechanical allodynia and thermal hyperalgesia. Given that CARM1 inhibition or knockdown attenuated the induction and maintenance of neuropathic pain after peripheral nerve injury, our findings suggest that CARM1 may serve as a promising therapeutic target f...
Source: Neuroscience - November 2, 2018 Category: Neuroscience Source Type: research

RNA-targeted therapeutics for lipid disorders
Purpose of review To summarize recent developments in the field of RNA-directed therapeutics targeting lipid disorders that are not effectively managed. Recent findings Despite a number of approved therapies for lipid disorders, significant unmet needs are present in treating persistently elevated LDL-cholesterol, remnant-cholesterol, triglycerides and lipoprotein(a) [Lp(a)]. Small molecules and antibodies are effective modalities, but they are unable to adequately treat many patients with abnormal lipid parameters. Targeting mRNA with oligonucleotides to prevent protein translation is a relatively novel method to red...
Source: Current Opinion in Lipidology - November 2, 2018 Category: Lipidology Tags: THERAPY AND CLINICAL TRIALS: Edited by Erik S.G. Stroes and Gerald F. Watts Source Type: research