Query: siRNA

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CDK12-mediated transcriptional regulation of noncanonical NF-{kappa}B components is essential for signaling
Members of the family of nuclear factor B (NF-B) transcription factors are critical for multiple cellular processes, including regulating innate and adaptive immune responses, cell proliferation, and cell survival. Canonical NF-B complexes are retained in the cytoplasm by the inhibitory protein IBα, whereas noncanonical NF-B complexes are retained by p100. Although activation of canonical NF-B signaling through the IBα kinase complex is well studied, few regulators of the NF-B–inducing kinase (NIK)–dependent processing of noncanonical p100 to p52 and the subsequent nuclear translocation of p52 have ...
Source: Signal Transduction Knowledge Environment - July 31, 2018 Category: Science Authors: Henry, K. L., Kellner, D., Bajrami, B., Anderson, J. E., Beyna, M., Bhisetti, G., Cameron, T., Capacci, A. G., Bertolotti-Ciarlet, A., Feng, J., Gao, B., Hopkins, B., Jenkins, T., Li, K., May-Dracka, T., Murugan, P., Wei, R., Zeng, W., Allaire, N., Buckle Tags: STKE Research Articles Source Type: news

LanCL1 attenuates ischemia-induced oxidative stress by Sirt3-mediated preservation of mitochondrial function.
Abstract Lanthionine synthetase C-like protein 1 (LanCL1) is homologous to prokaryotic lanthionine cyclases, and has been shown to have novel functions in neuronal redox homeostasis. A recent study showed that LanCL1 expression was developmental and activity-dependent regulated, and LanCL1 transgene protected neurons against oxidative stress. In the present study, the potential protective effects of LanCL1 against ischemia was investigated in an in vitro model mimicked by oxygen and glucose deprivation (OGD) in neuronal HT22 cells. We found that OGD exposure induced a temporal increase and persistent decreases in ...
Source: Brain Research Bulletin - July 31, 2018 Category: Neurology Authors: Xie Z, Cao BQ, Wang T, Lei Q, Kang T, Ge CY, Gao WJ, Hui H Tags: Brain Res Bull Source Type: research

Dendrobium moniliforme stem extract inhibits lipoteichoic acid-induced inflammatory responses by upregulation of heme oxygenase-1.
In this study, we investigated anti-inflammatory effects of the aqueous extract of D. moniliforme stems (DM) in response to lipoteichoic acid (LTA), a major constituent of the cell wall of Gram-positive bacteria. DM inhibited LTA-induced expression of a pro-inflammatory mediator inducible nitric oxide synthase (iNOS) in the murine macrophages. And DM induced expression of heme oxygenase-1 (HO-1) at the transcriptional level. Conversely, the knockdown of HO-1 expression by siRNA markedly reversed the inhibitory effects of DM on LTA-induced iNOS expression. We also demonstrated that nuclear translocation of Nrf2 was increase...
Source: Journal of Microbiology and Biotechnology - July 31, 2018 Category: Biotechnology Authors: Lee YJ, Kim JH, Kim Y Tags: J Microbiol Biotechnol Source Type: research

CDK12-mediated transcriptional regulation of noncanonical NF- κB components is essential for signaling.
CDK12-mediated transcriptional regulation of noncanonical NF-κB components is essential for signaling. Sci Signal. 2018 Jul 31;11(541): Authors: Henry KL, Kellner D, Bajrami B, Anderson JE, Beyna M, Bhisetti G, Cameron T, Capacci AG, Bertolotti-Ciarlet A, Feng J, Gao B, Hopkins B, Jenkins T, Li K, May-Dracka T, Murugan P, Wei R, Zeng W, Allaire N, Buckler A, Loh C, Juhasz P, Lucas B, Ennis KA, Vollman E, Cahir-McFarland E, Hett EC, Ols ML Abstract Members of the family of nuclear factor κB (NF-κB) transcription factors are critical for multiple cellular processes, including regulating innate and ad...
Source: Science Signaling - July 31, 2018 Category: Biomedical Science Authors: Henry KL, Kellner D, Bajrami B, Anderson JE, Beyna M, Bhisetti G, Cameron T, Capacci AG, Bertolotti-Ciarlet A, Feng J, Gao B, Hopkins B, Jenkins T, Li K, May-Dracka T, Murugan P, Wei R, Zeng W, Allaire N, Buckler A, Loh C, Juhasz P, Lucas B, Ennis KA, Vol Tags: Sci Signal Source Type: research

Small interfering RNA pathway contributes to antiviral immunity in Spodoptera frugiperda (Sf9) cells following Autographa californica multiple nucleopolyhedrovirus infection.
Abstract Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a well-known virus in the Baculoviridae family. Presence of the p35 gene in the AcMNPV genome as a suppressor of the short interfering RNA (siRNA) pathway is a strong reason for the importance of the siRNA pathway in the host cellular defense. Given that, here we explored the roles of Dicer-2 (Dcr2) and Argonaute 2 (Ago2) genes, key factors in the siRNA pathway in response to AcMNPV infection in Spodoptera frugiperda Sf9 cells. The results showed that the transcript levels of Dcr2 and Ago2 increased in response to AcMNPV infection particular...
Source: Insect Biochemistry and Molecular Biology - July 31, 2018 Category: Biochemistry Authors: Karamipour N, Fathipour Y, Talebi AA, Asgari S, Mehrabadi M Tags: Insect Biochem Mol Biol Source Type: research

Combination of NF-kB targeted siRNA and methotrexate in a hybrid nanocarrier towards the effective treatment in rheumatoid arthritis
The transcription factor NF-kB plays an important role in the pathogenesis of rheumatoid arthritis (RA). Effective treatment of RA is hindered due to the lack of specificity of small molecules in the inflamed ...
Source: Journal of Nanobiotechnology - July 30, 2018 Category: Nanotechnology Authors: Weifeng Duan and Huan Li Tags: Research Source Type: research

Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway.
CONCLUSIONS: Quercetin inhibits Ox-LDL-induced VSMC calcification in VSMCs possibly by targeting the ROS/TLR4 signaling pathway. PMID: 30187880 [PubMed - in process]
Source: Journal of Southern Medical University - July 30, 2018 Category: Universities & Medical Training Authors: Liang Q, Chen Y, Li C, Lu L Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

USP33 suppresses lung adenocarcinoma lung cell invasion and metastasis by down-regulating SLIT2/ROBO1 signaling pathway.
CONCLUSIONS: USP33 silencing can promote the migration, invasion and metastasis of lung adenocarcinoma cells in vitro, and the mechanism may involve IL-6 and SLIT2/ROBO1 signaling pathways. PMID: 30187867 [PubMed - in process]
Source: Journal of Southern Medical University - July 30, 2018 Category: Universities & Medical Training Authors: Wang Y, Zhang S, Mu S, Zhang B, Ma S Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

Destination Penis? Erectile Dysfunction As Possible Future Indication Of Therapeutic Gene Delivery.
Abstract Erectile Dysfunction (ED) is a common health problem in roughly 50% of males of advanced age (40-70 years old). Recent attention related gene therapy to ED cases; this received much interest to further progress gene therapy ideals to ED treatment. This review is an attempt to analyze key challenges and to emphasize primary areas, including mostly preclinical and few clinical trials, cellular target(s), and different viral vectors/nanoparticles for gene delivery in ED. While overexpression of target genes can be silenced by RNA interference (RNAi), down-regulation of these mechanisms has been implicated in...
Source: Current Gene Therapy - July 29, 2018 Category: Genetics & Stem Cells Authors: Gur S, Abdel-Mageed AB, Sikka SC, Bartolome AR, Hellstrom WJG Tags: Curr Gene Ther Source Type: research

Acetyl-11-keto- β-boswellic acid ameliorates renal interstitial fibrosis via Klotho/TGF-β/Smad signalling pathway.
In conclusion, our findings provide evidence that AKBA can effectively protect kidney against interstitial fibrosis, and this renoprotective effect involves the Klotho/TGF-β/Smad signalling pathway. Therefore, AKBA could be considered as a promising candidate drug for renal interstitial fibrosis. PMID: 30054990 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - July 28, 2018 Category: Molecular Biology Authors: Liu M, Liu T, Shang P, Zhang Y, Liu L, Liu T, Sun S Tags: J Cell Mol Med Source Type: research

Bispecific therapeutic aptamers for targeted therapy of cancer: a review on cellular perspective
AbstractAptamers (Aps), as short single-strand nucleic acids, can bind to their corresponding molecular targets with the high affinity and specificity. In comparison with the monoclonal antibodies (mAbs) and peptides, unique physicochemical and biological characteristics of Aps make them excellent targeting agents for different types of cancer molecular markers (CMMs). Much attention has been paid to the Ap-based multifunctional chimeric and therapeutic systems, which provide promising outcomes in the targeted therapy of various formidable diseases, including malignancies. In the Ap-based chimeric systems, a targeting Ap i...
Source: Journal of Molecular Medicine - July 28, 2018 Category: Molecular Biology Source Type: research

The atherogenic actions of LPC on vascular smooth muscle cells and its LPA receptor mediated mechanism.
Abstract Lysophosphatidylcholine (LPC) is a bioactive lipid constituent of oxidized low density lipoprotein (ox-LDL). It regulates various cellular functions, including migration of circulating monocytes, expression of endothelial adhesion molecules, proliferation and migration of vascular smooth muscle cells (VSMCs). LPC can also be hydrolyzed into lysophosphatidic acid (LPA) by autotaxin (ATX) which possesses lysophospholipase D (lyso-PLD) activity. The aim of this study was to explore the effects of LPC on proliferation and migration of human artery smooth muscle cells (HASMCs) and the involvement of LPC-ATX-LP...
Source: Biochemical and Biophysical Research communications - July 28, 2018 Category: Biochemistry Authors: Bao L, Qi J, Wang YW, Xi Q, Tserennadmid T, Zhao PF, Qi J, Damirin A Tags: Biochem Biophys Res Commun Source Type: research

Loss of histone deacetylase 2 inhibits oxidative stress induced by high glucose via the HO-1/SIRT1 pathway in endothelial progenitor cells.
In conclusion, these findings indicate that an HDAC2 inhibitor may prevent impaired cell proliferation, tube formation, inflammation, and ROS production in high-glucose EPCs. PMID: 30063937 [PubMed - as supplied by publisher]
Source: Gene - July 28, 2018 Category: Genetics & Stem Cells Authors: Gao J, Wang Y, Li W, Zhang J, Che Y, Cui X, Sun B, Zhao G Tags: Gene Source Type: research

Xihuang Pill Induces Apoptosis of Human Glioblastoma U-87 MG Cells via Targeting ROS-Mediated Akt/mTOR/FOXO1 Pathway.
In conclusion, we demonstrate for the first time that XHP may regulate glioblastoma U-87 MG cell apoptosis via ROS-mediated Akt/mTOR/FOXO1 pathway. PMID: 30046342 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - July 27, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells
In conclusion, our data illustrate a novel epigenetic pathway that links PERP transcription to ROS-induced apoptosis in skeletal muscle cells.
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - July 27, 2018 Category: Genetics & Stem Cells Source Type: research

Aquaporin 1 suppresses apoptosis and affects prognosis in esophageal squamous cell carcinoma.
In conclusion, the results of the present study suggested that the cytoplasm dominant expression of AQP1 is related to a poor prognosis in patients with ESCC, and that it activates tumor progression by affecting Death receptor signaling pathway. These results provide insights into the role of AQP1 as a mediator of and/or a biomarker for ESCC. PMID: 30042826 [PubMed]
Source: Oncotarget - July 27, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Targeted RNA/DNA Nanoparticles with Single Stranded RNA Toeholds
The development of RNA-based nanostructures and their use in a variety of applications, including RNA interference (RNAi) and drug delivery, represents an emerging field of science, technology, and biomedicine.   RNA is a dynamic material because of its natural functionalities, its ability to fold into complex small structures, and its capacity to self-assemble.  Taking advantage of these characteristic, NCI Researchers have improved upon their existing invention of multi-functional RNA/DNA nanoparticles by adding a RNA toehold instead of a DNA toehold.   In in vitro studies, they have shown that the nanoparticles are ...
Source: NIH OTT Licensing Opportunities - July 27, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Annexin A2 binds to vimentin and contributes to porcine reproductive and respiratory syndrome virus multiplication
In conclusion, ANXA2 c an interact with vimentin and enhance PRRSV growth. This contributes to the regulation of PRRSV replication in infected cells and may have implications for the future antiviral strategies.
Source: Veterinary Research - July 27, 2018 Category: Veterinary Research Source Type: research

RNA-Dependent Epigenetic Silencing Directs Transcriptional Downregulation Caused by Intronic Repeat Expansions
Publication date: Available online 26 July 2018Source: CellAuthor(s): Hannes Eimer, Sridevi Sureshkumar, Avilash Singh Yadav, Calvin Kraupner-Taylor, Champa Bandaranayake, Andrei Seleznev, Tamblyn Thomason, Stephen J. Fletcher, Stephanie Frances Gordon, Bernard J. Carroll, Sureshkumar BalasubramanianSummaryTranscriptional downregulation caused by intronic triplet repeat expansions underlies diseases such as Friedreich’s ataxia. This downregulation of gene expression is coupled with epigenetic changes, but the underlying mechanisms are unknown. Here, we show that an intronic GAA/TTC triplet expansion within the IIL1 gene...
Source: Cell - July 26, 2018 Category: Cytology Source Type: research

MicroRNA-449a suppresses hepatocellular carcinoma cell growth via G1 phase arrest and the HGF/c-Met pathway
ConclusionThe tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway.
Source: Hepatobiliary and Pancreatic Diseases International - July 26, 2018 Category: Gastroenterology Source Type: research

Effects of Fibronectin 1 on Cell Proliferation, Senescence and Apoptosis of Human Glioma Cells Through the PI3K/AKT Signaling Pathway
Conclusion: The findings of this study provide evidence highlighting the prominent role played by FN1 in stimulating glioma growth, invasion, and survival through the activation of the PI3K/AKT signaling pathway.Cell Physiol Biochem 2018;48:1382 –1396
Source: Cellular Physiology and Biochemistry - July 26, 2018 Category: Cytology Source Type: research

GSE117660 Caenorhabditis elegans heterochromatin factor SET-32 plays an essential role in transgenerational initiation of nuclear RNAi-mediated epigenetic silencing (sRNA-Seq)
Contributors : Natallia Kalinava ; Sam G GuSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Caenorhabditis elegansEpigenetic inheritance contributes fundamentally to transgenerational physiology and fitness. Mechanistic understanding of RNA-mediated chromatin modification and transgenerational epigenetic inheritance, which in C. elegans can be triggered by exogenous double-stranded RNA (exo-dsRNA) or facilitated by endogenous small interfering RNAs (endo-siRNAs), has mainly been limited to the post-initiation phases of silencing. Indeed, the dynamic process by which nuclear RNAi engages a tran...
Source: GEO: Gene Expression Omnibus - July 26, 2018 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Caenorhabditis elegans Source Type: research

GSE117659 Caenorhabditis elegans heterochromatin factor SET-32 plays an essential role in transgenerational initiation of nuclear RNAi-mediated epigenetic silencing (RNA-Seq)
Contributors : Natallia Kalinava ; Sam G GuSeries Type : Expression profiling by high throughput sequencingOrganism : Caenorhabditis elegansEpigenetic inheritance contributes fundamentally to transgenerational physiology and fitness. Mechanistic understanding of RNA-mediated chromatin modification and transgenerational epigenetic inheritance, which in C. elegans can be triggered by exogenous double-stranded RNA (exo-dsRNA) or facilitated by endogenous small interfering RNAs (endo-siRNAs), has mainly been limited to the post-initiation phases of silencing. Indeed, the dynamic process by which nuclear RNAi engages a transcri...
Source: GEO: Gene Expression Omnibus - July 26, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Caenorhabditis elegans Source Type: research

GSE117658 Caenorhabditis elegans heterochromatin factor SET-32 plays an essential role in transgenerational initiation of nuclear RNAi-mediated epigenetic silencing (ChIP-Seq)
Contributors : Natallia Kalinava ; Sam G GuSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Caenorhabditis elegansEpigenetic inheritance contributes fundamentally to transgenerational physiology and fitness. Mechanistic understanding of RNA-mediated chromatin modification and transgenerational epigenetic inheritance, which in C. elegans can be triggered by exogenous double-stranded RNA (exo-dsRNA) or facilitated by endogenous small interfering RNAs (endo-siRNAs), has mainly been limited to the post-initiation phases of silencing. Indeed, the dynamic process by which nuclear RNAi enga...
Source: GEO: Gene Expression Omnibus - July 26, 2018 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Caenorhabditis elegans Source Type: research

AMPK Activation Negatively Regulates GDAP1, which Influences the Metabolic Processes and Circadian Gene Expression in Skeletal Muscle
ConclusionWe describe a role for GDAP1 in regulating mitochondrial proteins, circadian genes, and metabolic flux in skeletal muscle. Collectively, our results implicate GDAP1 in the circadian control of metabolism.
Source: Molecular Metabolism - July 26, 2018 Category: Endocrinology Source Type: research

Polyester-based nanoparticles for nucleic acid delivery
Publication date: Available online 25 July 2018Source: Materials Science and Engineering: CAuthor(s): Jing Zhao, Guojun Weng, Jianjun Li, Jian Zhu, Junwu ZhaoAbstractGene therapy is promising for the treatment of inherited diseases and complex diseases such as chronic infections and cancer. The advancement in science and technology has extended the scope of gene therapy from gene transfer to the delivery of a variety of nucleic acids such as mRNA, antisense oligonucleotides (ASOs), siRNA and miRNA. Nanoparticle delivery systems can efficiently protect the nucleic acids from enzymatic degradation and immune recognition, fac...
Source: Materials Science and Engineering: C - July 26, 2018 Category: Materials Science Source Type: research

Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells.
In conclusion, our data illustrate a novel epigenetic pathway that links PERP transcription to ROS-induced apoptosis in skeletal muscle cells. PMID: 30056131 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - July 26, 2018 Category: Biochemistry Authors: Li Z, Chen B, Dong W, Xu W, Song M, Fang M, Guo J, Xu Y Tags: Biochim Biophys Acta Source Type: research

MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ER α and Survivin inhibition and enhances response to temozolomide.
MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERα and Survivin inhibition and enhances response to temozolomide. Oncotarget. 2018 Jul 03;9(51):29727-29742 Authors: Bobustuc GC, Kassam AB, Rovin RA, Jeudy S, Smith JS, Isley B, Singh M, Paranjpe A, Srivenugopal KS, Konduri SD Abstract The DNA damage repair enzyme, O6-methylguanine DNA methyltransferase (MGMT) is overexpressed in breast cancer, correlating directly with estrogen receptor (ER) expression and function. In ER negative breast cancer the MGMT promoter is frequently me...
Source: Oncotarget - July 25, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells.
In this study, we investigated the effects of PCP4/PEP19 on aromatase gene expression in MCF-7 and SK-BR-3 human breast cancer cells. In SK-BR-3 cells but not in MCF-7 cells, PCP4/PEP19 knockdown by siRNA silencing decreased the aromatase expression in gene transcriptional level. When PCP4/PEP19 was overexpressed by CMV promoter-driven PCP4/PEP19 expressing plasmid transfection, aromatase gene transcription increased in SK-BR-3 cells. This aromatase gene transcription is mainly mediated through promoter region PI.1, which is usually active in the placental tissue but not in the breast cancer tissue. These results indicate ...
Source: Oncotarget - July 25, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Schlafen 12 Interaction with SerpinB12 and Deubiquitylases Drives Human Enterocyte Differentiation
Conclusions: SLFN12 regulates human enterocytic differentiation by a pathway involving SERPB12, the deubiquitylases, and Cdx2. This pathway may be targeted to manipulate human enterocytic differentiation in mucosal atrophy, short gut or obesity.Cell Physiol Biochem 2018;48:1274 –1290
Source: Cellular Physiology and Biochemistry - July 25, 2018 Category: Cytology Source Type: research

Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation
Conclusion: Our results demonstrate for the first time that AGP induces RASSF1A expression in colon cancer cells and is dependent on angiogenesis signaling events. Therefore, our research may facilitate novel therapeutic options for advanced colon cancer therapy.Cell Physiol Biochem 2018;48:1259 –1273
Source: Cellular Physiology and Biochemistry - July 25, 2018 Category: Cytology Source Type: research

Long non-coding RNA TRPM2-AS as a potential biomarker for hepatocellular carcinoma
ConclusionLong non-coding RNA TRPM2-AS is upregulated in HCC and represents a new biomarker for HCC and the inhibition of TRPM2-AS promotes apoptosis in HCC cells in vitro.
Source: Irish Journal of Medical Science - July 25, 2018 Category: General Medicine Source Type: research

Engineering of budesonide-loaded lipid-polymer hybrid nanoparticles using a quality-by-design approach
This study shows the importance of systematic formulation design for understanding the effect of formulation parameters on the characteristics of LPNs, eventually resulting in the identification of an OOS.Graphical abstract
Source: International Journal of Pharmaceutics - July 25, 2018 Category: Drugs & Pharmacology Source Type: research

GSE113923 RNA-dependent epigenetic silencing directs transcriptional down regulation caused by intronic repeat expansions
Contributors : Hannes Eimer ; Sridevi Sureshkumar ; Avilash S Yadav ; Calvin Kraupner-Taylor ; Champa Bandaranayake ; Andrei Seleznev ; Tamblyn Thomason ; Stephen Fletcher ; Stephanie F Gordon ; Bernard J Carroll ; Sureshkumar BalasubramanianSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaTranscriptional down regulation caused by intronic triplet repeat expansions underlies diseases such as Friedreich?s ataxia. This down regulation of gene expression is coupled with epigenetic changes but the underlying mechanisms are unknown. Here, we show that an intronic TTC/GAA triplet exp...
Source: GEO: Gene Expression Omnibus - July 25, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

Targeting autophagy by small molecule inhibitors of vacuolar protein sorting 34 (Vps34) improves the sensitivity of breast cancer cells to Sunitinib
In this study we systematically screened a library of 306 known anti-cancer drugs for their ability to induce autophagy using a cell-based assay. 114 of the drugs were classified as autophagy inducers; for 16 drugs, the cytotoxicity was potentiated by siRNA-mediated knock-down of Atg7 and Vps34. These drugs were further evaluated in breast cancer cell lines for autophagy induction, and two tyrosine kinase inhibitors, Sunitinib and Erlotinib, were selected for further studies.
Source: Cancer Letters - July 25, 2018 Category: Cancer & Oncology Authors: Matheus Dyczynski, Yasmin Yu, Magdalena Otrocka, Santiago Parpal, Tiago Braga, Aine Brigette Henley, Henric Zazzi, Mikael Lerner, Krister Wennerberg, Jenny Viklund, Jessica Martinsson, Dan Grand ér, Angelo De Milito, Katja Pokrovskaja Tamm Tags: Original Articles Source Type: research

Glucosamine abrogates the stem cell factor  + endothelin-1-induced stimulation of melanogenesis via a deficiency in MITF expression due to the proteolytic degradation of CREB in human melanocytes
In this study, we characterized the molecular mechanism involved in the anti-melanogenic effects of GlcN using normal human melanocytes (NHMs) in culture. The SE-stimulated gene (12 h) and protein (24 h) expression levels of melanocyte-specific proteins (at the indicated times post-stimulation) were significantly abrogated by pretreatment with GlcN for 72 h. Western blotting analysis of the phosphorylation of intracellular signaling molecules in the MAPK pathway revealed that despite the significantly decreased level of total CREB protein at all times post-stimulat ion, the SE-stimulated phosphorylation of ERK, CREB and...
Source: Archives of Dermatological Research - July 25, 2018 Category: Dermatology Source Type: research

O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death
In this study, co-incubation of HepG2 cells with E. histolytica increased DNA fragmentation and LDH release as compared with control cells. Additionally, Gal-lectin-mediated amoebic adherence of live trophozoites to HepG2 cells decreased O-GlcNAcylated protein levels within 5 min. We also observed a rapid decrease in cellular OGT protein level, but not OGA, in HepG2 cells in a contact-dependent manner. Furthermore, HepG2 pretreatment with OGA inhibitors or OGA siRNA prevented E. histolytica-induced O-deGlcNAcylation, DNA fragmentation, and LDH release. Our results suggested that E. histolytica-induced O-deGlcNAcylation i...
Source: Microbial Pathogenesis - July 24, 2018 Category: Infectious Diseases Source Type: research

Expression of BANCR promotes papillary thyroid cancer by targeting thyroid stimulating hormone receptor.
Authors: Zheng H, Xu J, Hao S, Liu X, Ning J, Song X, Jiang L, Liu Z Abstract Papillary thyroid carcinoma (PTC) is the most common form of non-medullary thyroid cancer, accounting for ~80% of all cases of thyroid cancer. The aim of the present study was to explore the role of BRAF-activated long noncoding RNA (BANCR) in the development of PTC. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the mRNA expression levels of BANCR, thyroid-stimulating hormone receptor (TSHR) and cyclin D1 between PTC and benign control thyroid nodule tissue samples from 60 patients were determined. Using RT...
Source: Oncology Letters - July 24, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Roles of the MST1-JNK signaling pathway in apoptosis of colorectal cancer cells induced by Taurine.
Authors: Liu Z, Xia Y, Zhang X, Liu L, Tu S, Zhu W, Yu L, Wan H, Yu B, Wan F Abstract The aim of this study was to observe the impact of the mammalian sterile 20-like kinase 1-c-Jun N-terminal kinase (MST1-JNK) signaling pathway on apoptosis in colorectal cancer (CRC) cells induced by Taurine (Tau). Caco-2 and SW620 cells transfected with p-enhanced green fluorescent protein (EGFP)-MST1 or short interfering RNA (siRNA)-MST1 were treated with Tau for 48 h. Apoptosis was detected by flow cytometry, and the levels of MST1 and JNK were detected by western blotting. Compared with the control group, 80 mM Tau could sig...
Source: Libyan Journal of Medicine - July 24, 2018 Category: General Medicine Tags: Libyan J Med Source Type: research

The prostaglandin EP4 receptor is a master regulator of the expression of PGE2 receptors following inflammatory activation in human monocytic cells
Publication date: Available online 24 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Alaa Kashmiry, Rothwelle Tate, Giuliana Rotondo, Jillian Davidson, Dino RotondoAbstractProstaglandin E2 (PGE2) is responsible for inflammatory symptoms. However, PGE2 also suppresses pro-inflammatory cytokine production. There are at least 4 subtypes of PGE2 receptors, EP1–EP4, but it is unclear which of these specifically control cytokine production. The aim of this study was to determine which of the different receptors, EP1R–EP4R modulate production of tumor necrosis factor-α (...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 24, 2018 Category: Lipidology Source Type: research

MiR-126-3p inhibits ovarian cancer proliferation and invasion via targeting PLXNB2.
Abstract Ovarian cancer is one of the leading malignancies in women and the 5-year survival rate of ovarian cancer still remains poor. In the present study, we aimed to investigate the interaction between the miR-126-3p and PLXNB2 in the progression of ovarian cancer. The qRT-PCR data revealed a reduction of miR-126-3p level in ovarian cancer tissues comparing to the adjacent normal tissues. Over-expression of miR-126-3p in ovarian cancer cells suppressed cell proliferation and invasion and the phosphorylation of AKT and ERK1/2. The cell cycle assay results showed that the over-expression of miR-126-3p induced cel...
Source: Reproductive Biology - July 24, 2018 Category: Reproduction Medicine Authors: Xiang G, Cheng Y Tags: Reprod Biol Source Type: research

TRIM65 is a potential oncogenic protein via ERK1/2 on Jurkat and Raji cells: a therapeutic target in human lymphoma malignancies.
Abstract In human lung cancer, Tripartite motif 65 (TRIM65) is documented as an important regulator in carcinogenesis. Knockdown of TRIM65 prevents the tumorigenesis of lung cancer cells, while TRIM65 overexpression presents the opposite effect. However, the roles of TRIM65 in human lymphocyte malignancies have reported little. Herein, we found that Jurkat (T-lymphocyte) and Raji (B-lymphocyte) expressed TRIM65. We aimed to investigate whether TRIM65 was a potential oncogenic protein that regulated the tumorigenesis of Jurkat and Raji cells. In our present study, cells were transfected with siRNA-TRIM65 or TRIM65 ...
Source: Cell Biology International - July 24, 2018 Category: Cytology Authors: Wang J, Liang X, Yu T, Xu YL, Xu LH, Zhang XJ, Ma J, Wang YR, He SL Tags: Cell Biol Int Source Type: research

Up-regulation of TRAF2 inhibits chondrocytes apoptosis in lumbar facet joint osteoarthritis.
In conclusion, our results show that the up-regulation of TRAF2 may play an important role in the inhibition of chondrocyte apoptosis of FJOA. PMID: 30054040 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - July 24, 2018 Category: Biochemistry Authors: Jiang J, Zhang J, Wu C, Guo X, Chen C, Bao G, Sun Y, Chen J, Xue P, Xu G, Cui Z Tags: Biochem Biophys Res Commun Source Type: research

The prostaglandin EP4 receptor is a master regulator of the expression of PGE2 receptors following inflammatory activation in human monocytic cells.
Abstract Prostaglandin E2 (PGE2) is responsible for inflammatory symptoms. However, PGE2 also suppresses pro-inflammatory cytokine production. There are at least 4 subtypes of PGE2 receptors, EP1-EP4, but it is unclear which of these specifically control cytokine production. The aim of this study was to determine which of the different receptors, EP1R-EP4R modulate production of tumor necrosis factor-α (TNF-α) in human monocytic cells. Human blood, or the human monocytic cell line THP-1 were stimulated with LPS. The actions of PGE2, alongside both selective agonists of EP1-EP4 receptors, were assessed on LPS-ind...
Source: Biochimica et Biophysica Acta - July 24, 2018 Category: Biochemistry Authors: Kashmiry A, Tate R, Rotondo G, Davidson J, Rotondo D Tags: Biochim Biophys Acta Source Type: research

Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling.
CONCLUSIONS: HO-1 ameliorated endothelial senescence through enhancing eNOS activation and defending eNOS uncoupling, suggesting that HO-1 is a potential target for treating endothelial senescence. PMID: 30048241 [PubMed - as supplied by publisher]
Source: Aging - July 24, 2018 Category: Biomedical Science Authors: Luo W, Wang Y, Yang H, Dai C, Hong H, Li J, Liu Z, Guo Z, Chen X, He P, Li Z, Li F, Jiang J, Liu P, Li Z Tags: Aging (Albany NY) Source Type: research

Trefoil factor 1 inhibits epithelial-mesenchymal transition of pancreatic intraepithelial neoplasm
This study indicates that the acquisition of TFF1 expression is an early event in pancreatic carcinogenesis and that TFF1 might act as a tumor suppressor to prevent EMT and the invasive transformation of PanIN.
Source: Journal of Clinical Investigation - July 23, 2018 Category: Biomedical Science Authors: Junpei Yamaguchi, Yukihiro Yokoyama, Toshio Kokuryo, Tomoki Ebata, Atsushi Enomoto, Masato Nagino Source Type: research

Metformin Alleviates Radiation-Induced Skin Fibrosis via the Downregulation of FOXO3
Conclusions: The results indicated that metformin suppresses radiation-induced skin injuries by modulating the expression of FOXO3 through PIK3r1. Collectively, the data obtained in this study suggested that metformin could be a potent therapeutic agent for alleviating radiation-induced skin fibrosis.Cell Physiol Biochem 2018;48:959 –970
Source: Cellular Physiology and Biochemistry - July 23, 2018 Category: Cytology Source Type: research

RANK Deficiency Ameliorates Podocyte Injury by Suppressing Calcium/Calcineurin/ NFATc1 Signaling
Conclusion: Our findings demonstrated that RANK deficiency ameliorates podocyte injury by suppressing calcium/calcineurin/NFATc1 signaling, which may present a promising target for therapeutic intervention.Kidney Blood Press Res 2018;43:1149 –1159
Source: Kidney and Blood Pressure Research - July 23, 2018 Category: Urology & Nephrology Source Type: research

ASAP Thiol-Epoxy “Click” Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b07167
Source: ACS Applied Materials and Interfaces - July 23, 2018 Category: Materials Science Authors: Cong Truc Huynh, Fangze Liu, Yuxuan Cheng, Katherine A. Coughlin, Eben Alsberg Source Type: research

Development and characterization of a novel cationic PEGylated niosome-encapsulated forms of doxorubicin, quercetin and siRNA for the treatment of cancer by using combination therapy
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Source: Artificial Cells, Blood Substitutes, and Biotechnology - July 23, 2018 Category: Biotechnology Authors: Mahdie Hemati Fateme Haghiralsadat Fatemeh Yazdian Farzaneh Jafari Ali Moradi Zahra Malekpour-Dehkordi Source Type: research