Query: siRNA

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Total 13804 results found since Jan 2013.

Cell-Specific Functions of ADAM17 Regulate the Progression of Thoracic Aortic Aneurysm.
Conclusions: We have identified distinct cell-specific functions of ADAM17 in TAA progression, promoting pathological remodeling of SMC and impairing integrity of the intimal EC barrier. The dual impact of ADAM17 deficiency (or inhibition) in protecting two major cell types in the aortic wall highlights the unique position of this proteinase as a critical treatment target for TAA. PMID: 29930147 [PubMed - as supplied by publisher]
Source: Circulation Research - June 21, 2018 Category: Cardiology Authors: Shen M, Hu M, Fedak PW, Oudit GY, Kassiri Z Tags: Circ Res Source Type: research

Silencing Ubc9 expression suppresses osteosarcoma tumorigenesis and enhances chemosensitivity to HSV-TK/GCV by regulating connexin  43 SUMOylation.
In conclusion, the present study revealed a novel method for the effective restoration of GJIC in osteosarcoma cells, which may increase their sensitivity to conventional chemotherapy. PMID: 29956745 [PubMed - as supplied by publisher]
Source: International Journal of Oncology - June 21, 2018 Category: Cancer & Oncology Authors: Zhang D, Yu K, Yang Z, Li Y, Ma X, Bian X, Liu F, Li L, Liu X, Wu W Tags: Int J Oncol Source Type: research

The anti-apoptotic ubiquitin conjugating enzyme BIRC6/BRUCE regulates autophagosome-lysosome fusion.
Abstract The Inhibitor of Apoptosis (IAP) family member, BIRC6/BRUCE is a ubiquitin conjugating E2 enzyme and a well-established anti-apoptosis regulator. However, its role in mammalian autophagy has not been shown. We identified BIRC6 as an important positive regulator of macroautophagy/autophagy by performing an siRNA screen targeting enzymes in the ubiquitin pathway. Compared to wild-type cells, BIRC6-deficient cells show accumulation of lipidated LC3B both at basal and starved conditions. Furthermore, BIRC6 deficiency blocks starvation-induced autophagic flux monitored by a tandem fluorescent autophagy sensor,...
Source: Autophagy - June 21, 2018 Category: Cytology Authors: Ikeda F Tags: Autophagy Source Type: research

Up-regulation of microRNA-503 by high glucose reduces the migration and proliferation but promotes the apoptosis of human umbilical vein endothelial cells by inhibiting the expression of insulin-like growth factor-1 receptor.
CONCLUSIONS: The present study demonstrates that miR-503 expression in HUVECs is elevated in high glucose condition. Also, miR-503 reduces migration and proliferation, but promotes apoptosis of HUVECs by inhibiting IGF-1R expression. PMID: 29917206 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Sestrin2 aggravates oxidative stress of neurons by decreasing the expression of Nrf2.
CONCLUSIONS: Sesn2 promoted the transfer of nuclear Nrf2 to the cytoplasm, it decreased the expressions of Nrf2 and its downstream proteins, Srx1 and Trx1. Meanwhile, it increased the cerebral ischemia-reperfusion injury by changing the distribution of Nrf2. PMID: 29917204 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MiR-143 regulates proliferation and apoptosis of myelocytic leukemia cell HL-60 via modulating ERK1.
CONCLUSIONS: MiR-143 down-regulation and ERK1 up-regulation are correlated with APL pathogenesis. Their expression level affected patient's prognosis. MiR-143 targeted and inhibited ERK1 expression, weakened proliferation potency of HL-60 cells, and induced apoptosis. PMID: 29917183 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Mir-98 reduces the expression of HMGA2 and promotes osteogenic differentiation of mesenchymal stem cells.
CONCLUSIONS: MiR-98 can promote osteogenic differentiation of mesenchymal stem cells by targeting gene HMGA2. PMID: 29917180 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

The role of MiR-324-3p in polycystic ovary syndrome (PCOS) via targeting WNT2B.
CONCLUSIONS: The expression of miR-324-3p in the ovary of PCOS rats is decreased significantly. Overexpression of miR-324-3p can reduce the proliferation and induce the apoptosis of granulosa cells via targeting of WNT2B. PMID: 29917177 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells
In conclusion, the present study demonstrated that LASS2 inhibits bladder cancer invasion and chemoresistance through regulation of ERK-Drp1 induced mitochondrial dynamics.
Source: Journal of Cancer - June 20, 2018 Category: Cancer & Oncology Authors: Lijuan Huang, Ting Luan, Yujin Chen, Xin Bao, Yinglong Huang, Shi Fu, Haifeng Wang, Jiansong Wang Tags: Research Paper Source Type: research

Aminoacylase 3 Is a New Potential Marker and Therapeutic Target in Hepatocellular Carcinoma
Ras proteins (HRas, KRas and NRas) are common oncogenes that require membrane association for activation. Previous approaches to block/inhibit Ras membrane association were unsuccessful for cancer treatment in human clinical studies. In the present study we utilized a new approach to decrease Ras membrane association in hepatocellular carcinoma (HCC) cell lines via inhibition of an enzyme aminoacylase 3 (AA3; EC 3.5.1.114). AA3 expression was significantly elevated in the livers of HCC patients and HCC cell lines. Treatment of HepG2 cells with AA3 inhibitors, and HepG2 and HuH7 with AA3 siRNA significantly decreased Ras me...
Source: Journal of Cancer - June 20, 2018 Category: Cancer & Oncology Authors: Kirill Tsirulnikov, Sergio Duarte, Anamika Ray, Nakul Datta, Ali Zarrinpar, Lin Hwang, Kym Faull, Alexander Pushkin, Ira Kurtz Tags: Research Paper Source Type: research

A Study on Effect of Oxaliplatin in MicroRNA Expression in Human Colon Cancer
This study outlines the regulatory effects of oxaliplatin on miRNAs expression in colon cancer cells and correlates it with the changing microRNA expression with p53 and p73 expression status in cells. HCT116p53+/+ and HCT116p53-/- cells were exposed to oxaliplatin, and the cellular viability was determined by XTT. p73 was knocked down using siRNA and the tumor cells were then treated with oxaliplatin. The expression profile of 384 miRNAs was determined by TaqMan® human miRNA array and calculated by the ∆∆Ct method. Cellular viability was found to decrease after the treatment with oxaliplatin in a dose-dep...
Source: Journal of Cancer - June 20, 2018 Category: Cancer & Oncology Authors: Jasmine Evert, Surajit Pathak, Xiao-Feng Sun, Hong Zhang Tags: Research Paper Source Type: research

XIAP Limits Autophagic Degradation of Sox2 and Is A Therapeutic Target in Nasopharyngeal Carcinoma Stem Cells
Conclusion: Our study highlights the importance of IAPs in the maintenance of CSCs in NPC. Thus, XIAP is a promising therapeutic target in CSCs and suggests that NPC patients may benefit from a combination treatment of APG-1387 with conventional chemotherapy.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Jiao Ji, Yan Yu, Zhi-Ling Li, Ming-Yuan Chen, Rong Deng, Xiang Huang, Guang-Feng Wang, Meng-Xia Zhang, Qi Yang, Senthilkumar Ravichandran, Gong-Kan Feng, Xue-Lian Xu, Chen-Lu Yang, Miao-Zhen Qiu, Lin Jiao, Dajun Yang, Xiao-Feng Zhu Tags: Research Paper Source Type: research

PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy
Conclusion: These findings identify a novel role of PrLZ in autophagy manipulation and provide new insight into docetaxel chemoresistance in PCa, suggesting a new strategy for treating mCRPC by targeting this newly identified signaling pathway.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Jin Zeng, Wei Liu, Yi-Zeng Fan, Da-Lin He, Lei Li Tags: Research Paper Source Type: research

Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array
Rationale: Delivery of nucleic acid molecules into skin remains a main obstacle for various types of gene therapy or vaccine applications. Here we propose a novel electroporation approach via combined use of a microneedle roller and a flexible interdigitated electroporation array (FIEA) for efficient delivery of DNA and siRNA into mouse skin.Methods: Using micromachining technology, closely spaced gold electrodes were made on a pliable parylene substrate to form a patch-like electroporation array, which enabled close surface contact between the skin and electrodes. Pre-penetration of the skin with a microneedle roller resu...
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Dong Huang, Deyao Zhao, Xiaoxia Wang, Chunhui Li, Tongren Yang, Lili Du, Zewen Wei, Qiang Cheng, Huiqing Cao, Zicai Liang, Yuanyu Huang, Zhihong Li Tags: Research Paper Source Type: research

Long Non-coding MIR205HG Depletes Hsa-miR-590-3p Leading to Unrestrained Proliferation in Head and Neck Squamous Cell Carcinoma
Conclusions: Taken together, these findings identify a transcriptional and post-transcriptional molecular network that includes mutant p53 protein, lncMIR205HG, YAP, and other proliferation-related genes, which are enriched in HNSCC patients with poor prognosis.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Silvia Di Agostino, Fabio Valenti, Andrea Sacconi, Giulia Fontemaggi, Matteo Pallocca, Claudio Pulito, Federica Ganci, Paola Muti, Sabrina Strano, Giovanni Blandino Tags: Research Paper Source Type: research

Suspension state promotes metastasis of breast cancer cells by up-regulating cyclooxygenase-2
Conclusions: Our results demonstrate that suspension state plays an important role in the metastatic potential of CTCs, and suggest a potential application of COX-2 inhibitor for anti-metastasis.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Xiaomei Zhang, Li Yang, Shu Chien, Yonggang Lv Tags: Research Paper Source Type: research

Punching and Electroporation for Enhanced Transdermal Drug Delivery
Transdermal delivery has made an indispensable impact to medical practice, but often been limited by low efficiency due to the barrier of the outer stratum corneum layer of skin. In Issue 9 of Theranostics, Huang et al. [1] proposed a new design of transdermal gene delivery strategy via the combination of a microneedle roller and a flexible interdigitated electroporation array. With the assistance of the microneedle roller, a deep and uniform electric field in the skin can be formed, accompanying an enhanced transport efficiency even at a low voltage. Furthermore, this combination strategy can promote the gene expression a...
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Guang Yang, Yuqi Zhang, Zhen Gu Tags: Editorial Source Type: research

Flow-dependent epigenetic regulation of IGFBP5 expression by H3K27me3 contributes to endothelial anti-inflammatory effects
Conclusion: Taken together, our findings reveal that atheroprotective flow reduces H3K27me3 as a chromatin-based mechanism to augment the expression of genes that confer an anti-inflammatory response in the endothelium. Our study exemplifies flow-dependent epigenetic regulation of endothelial gene expression, and also suggests that targeting the EZH2/H3K27me3/IGFBP5 pathway may offer novel therapeutics for inflammatory disorders such as atherosclerosis.
Source: Theranostics - June 20, 2018 Category: Molecular Biology Authors: Suowen Xu, Yanni Xu, Meimei Yin, Shuya Zhang, Peng Liu, Marina Koroleva, Shuyi Si, Peter J. Little, Jaroslav Pelisek, Zheng Gen Jin Tags: Research Paper Source Type: research

Loss of the basic helix-loop-helix transcription factor Bhlhe41 induces cell death and impairs neurite outgrowth in Neuro2a cells.
Abstract The basic helix-loop-helix (bHLH) superfamily of transcription factors have been implicated in a wide range of cellular functions such as proliferation, differentiation, tumorigenesis, and circadian rhythms. In a previous siRNA-based screen, bHLH family member e41 (BHLHE41) had been identified as a putative regulator of neuronal differentiation; however, its function remains largely elusive. To this end, using the CRISPR-Cas9 system, we established an isogenic Neuro2a (N2a) cell line with biallelic targeting of Bhlhe41 gene (Bhlhe41-/-). In undifferentiated N2a cells, complete knockout of Bhlhe41 resulted...
Source: Molecular and Cellular Biochemistry - June 20, 2018 Category: Biochemistry Authors: Sun Y, Zhang H, Wang L, Li J, Jin H, Wang Z, Tian S, Qi L, Liu X Tags: Mol Cell Biochem Source Type: research

Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells.
In conclusion, OXY increases NAD+ levels, resulting in the stimulation of MUC2 expression in LS 174T cells. These findings present a novel role for NAD+ in stimulation of MUC2 expression. PMID: 29935245 [PubMed - as supplied by publisher]
Source: Food and Chemical Toxicology - June 20, 2018 Category: Food Science Authors: Hwang D, Jo H, Ma SH, Lim YH Tags: Food Chem Toxicol Source Type: research

Heat Shock Protein 72 Regulates Hepatic Lipid Accumulation.
Abstract Induction of the chaperone Heat Shock Protein 72 (HSP72) through heat treatment, exercise, or overexpression improves glucose tolerance and mitochondrial function in skeletal muscle. Less is known about HSP72 function in the liver where lipid accumulation can result in insulin resistance and Nonalcoholic Fatty Liver Disease (NAFLD). The purpose of this study was 1) to determine whether weekly in vivo heat treatment (HT) induces hepatic HSP72 and improves glucose tolerance in rats fed a high-fat diet (HFD) and 2) to determine the ability of HSP72 to protect against lipid accumulation and mitochondrial dysf...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - June 20, 2018 Category: Physiology Authors: Archer AE, Rogers RS, Von Schulze AT, Wheatley JL, Morris EM, McCoin CS, Thyfault JP, Geiger PC Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research

Prox1 is essential for oligodendrocyte survival and regulates oligodendrocyte apoptosis via the regulation of NOXA.
Abstract Demyelinating diseases, such as multiple sclerosis, are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. Its underlying molecular mechanisms, however, remain unclear. The transcription factor prospero homeobox 1 (Prox1) plays an essential role during embryonic development of the central nervous system and cell differentiation. Thus, we aimed to investigate the role of Prox1 in the survival and differentiation of oligodendrocytes. Cell viability was measured by MTT assay. Flow cytometry was conducted to analyze cell apoptosis. Ectopic-Prox1 and shProx1 were us...
Source: Acta Biochimica et Biophysica Sinica - June 20, 2018 Category: Biochemistry Authors: Chang W, Teng J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

HTT2 promotes plant thermotolerance in Brassica rapa
Numerous regulatory genes participate in plant thermotolerance. In Arabidopsis, HEAT-INDUCED TAS1 TARGET2 (HTT2) is an important thermotolerance gene that is silenced by ta-siR255, a trans-acting siRNA. ta-siR255...
Source: BMC Plant Biology - June 20, 2018 Category: Biology Authors: Jianxia Jiang, Jinjuan Bai, Shuxia Li, Xiaorong Li, Liyong Yang and Yuke He Tags: Research article Source Type: research

Regulatory effects of the long non ‑coding RNA RP11‑543N12.1 and microRNA‑324‑3p axis on the neuronal apoptosis induced by the inflammatory reactions of microglia.
In conclusion, RP11‑543N12.1 targeted miR‑324‑3p to suppress proliferation and promote apoptosis in the AD cell model, suggesting that RP11‑543N12.1 and miR‑324‑3p may be potential biomarkers and therapeutic targets for AD. PMID: 29956723 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - June 20, 2018 Category: Molecular Biology Authors: Cai M, Wang YW, Xu SH, Qiao S, Shu QF, Du JZ, Li YG, Liu XL Tags: Int J Mol Med Source Type: research

Intrarenal ghrelin receptor inhibition ameliorates angiotensin ii-dependent hypertension in rats.
INTRARENAL GHRELIN RECEPTOR INHIBITION AMELIORATES ANGIOTENSIN II-DEPENDENT HYPERTENSION IN RATS. Am J Physiol Renal Physiol. 2018 Jun 20;: Authors: Kemp BA, Howell NL, Padia SH Abstract The intrarenal ghrelin receptor (GR) is localized to collecting duct (CD) cells where it increases αENaC-dependent sodium reabsorption in rodents. We hypothesized that chronic GR inhibition with intrarenal GR siRNA lowers blood pressure (BP) in Angiotensin II-dependent hypertension via reductions in αENaC-dependent sodium reabsorption. Uninephrectomized Sprague-Dawley rats (N=121) received subcutaneous osmotic pumps...
Source: Am J Physiol Renal P... - June 20, 2018 Category: Urology & Nephrology Authors: Kemp BA, Howell NL, Padia SH Tags: Am J Physiol Renal Physiol Source Type: research

GSE100261 Transcriptional impact of MTHFD2 in Human Aortic Endothelial Cells
Contributors : Juliane Hitzel ; Eunjee Lee ; Jun Zhu ; Ralf P BrandesSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe performed transcriptome analysis of Human Aortic Endothelial Cells after siRNA mediated knockdown of MTHFD2. We identified MTHFD2 as a key driver for a gene cluster which integrates mitochondrial one-carbon metabolism, serine synthesizing enzymes as well as common amino acid and ER stress response genes.
Source: GEO: Gene Expression Omnibus - June 19, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

ASAP Improved Cell Transfection of siRNA by pH-Responsive Nanomicelles Self-Assembled with mPEG- < italic toggle="yes" > b < /italic > -PHis- < italic toggle="yes" > b < /italic > -PEI Copolymers
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b04301
Source: ACS Applied Materials and Interfaces - June 19, 2018 Category: Materials Science Authors: Bin Xu, Yuan-Jun Zhu, Cheng-Han Wang, Chong Qiu, Jing Sun, Yi Yan, Xin Chen, Jian-Cheng Wang, Qiang Zhang Source Type: research

DNA binding protein HMGB1 secreted by activated microglia promotes the apoptosis of hippocampal neurons in diabetes complicated with OSA
Publication date: Available online 18 June 2018 Source:Brain, Behavior, and Immunity Author(s): Yu Shi, Xiangyu Guo, Jie Zhang, Hanchi Zhou, Bei Sun, Jing Feng Type 2 diabetes mellitus (T2DM) complicated with obstructive sleep apnea (OSA) may cause neuronal apoptosis and cognitive deficits, but the underlying mechanisms remain unclear. We aimed to determine the relationship between the activation of microglia and the apoptosis of hippocampal neurons, specifically in terms of high mobility group box-1 (HMGB1), after high glucose (HG) and intermittent hypoxia (IH) exposure. Diabetic KK-Ay mice and non-diabetic C57BL/6J mice...
Source: Brain, Behavior, and Immunity - June 19, 2018 Category: Neurology Source Type: research

Redox-triggered intracellular siRNA delivery
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C8CC01376D, CommunicationNecibe Basaran Mutlu Agardan, Can Sarisozen, Vladimir Torchilin Stimuli-responsive nanopreparations are able to provide drug release in response to various physiological stimuli including redox potential. Gene silencing using small interfering RNA (siRNA) is a promising strategy for the... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - May 29, 2018 Category: Chemistry Authors: Necibe Basaran Mutlu Agardan Source Type: research

Galactose Derivative-Modified Nanoparticles for Efficient siRNA Delivery to Hepatocellular Carcinoma.
In this study, we assembled lipid/calcium/phosphate nanoparticles (LCP NPs) conjugated with eight types of galactoside derivatives and demonstrated that phenyl β-d-galactoside-decorated LCP NPs (L4-LCP NPs) exhibited a superior siRNA delivery into HCC cells compared to normal hepatocytes. VEGF siRNAs delivered by L4-LCP NPs downregulated VEGF expression in HCC in vitro and in vivo and led to a potent antiangiogenic effect in the tumor microenvironment of a murine orthotopic HCC model. The efficient delivery of VEGF siRNA by L4-LCP NPs that resulted in significant tumor regression indicates that phenyl galactoside could be...
Source: Biomacromolecules - May 29, 2018 Category: Biochemistry Authors: Huang KW, Lai YT, Chern GJ, Huang SF, Tsai CL, Sung YC, Chiang CC, Hwang PB, Ho TL, Huang RL, Shiue TY, Chen Y, Wang SK Tags: Biomacromolecules Source Type: research

The study of relationships between pKa value and siRNA delivery efficiency based on tri-block copolymers.
Abstract Tri-block copolymers have exhibited great potentials in small interfering RNA (siRNA) therapeutics. To reveal structure-activity relationships, we here synthesized a series of tri-block copolymers with different hydrophobic segments, PEG-PAMA-P(C6Ax-C7Ay-DPAz-DBAm) (EAAS) and PEG-PDAMAEMA-P(C6Ax-C7Ay-DPAz-DBAm) (EDAS), termed from EAASa to EAASh and EDASa to EDASh, with pKa ranging from 5.2 to 7.0. Our data showed that the better gene silencing efficiency was located in pKa of 5.8-6.2, which was contributed from higher endosomal escape observed with confocal images and hemolysis assay. EAASc, the leader p...
Source: Biomaterials - May 29, 2018 Category: Materials Science Authors: Du L, Wang C, Meng L, Cheng Q, Zhou J, Wang X, Zhao D, Zhang J, Deng L, Liang Z, Dong A, Cao H Tags: Biomaterials Source Type: research

Enhancing the therapeutic effect via elimination of hepatocellular carcinoma stem cells using Bmi1 siRNA delivered by cationic cisplatin Nanocapsules
Publication date: Available online 26 May 2018 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Tan Yang, Yuyuan Chen, Pengxuan Zhao, Huiying Xue, Jia You, Bin Li, Yong Liu, Chuanchuan He, Xiaojuan Zhang, Lingling Fan, Robert J. Lee, Lei Li, Xiang Ma, Chuanrui Xu, Guangya Xiang Resistance of hepatocellular carcinoma (HCC) to systemic chemotherapy is partially due to presence of drug-resistant cancer stem cells. Bmi1 protein is essential for survival and proliferation of HCC cancer stem cells (CSCs). Here, we report that Bmi1 siRNA (Bmi1siR) loaded in cationic nanocapsules of cisplatin (NPC) eliminated ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 27, 2018 Category: Nanotechnology Source Type: research

Poly (amidoamine) (PAMAM) dendrimer mediated delivery of drug and pDNA/siRNA for cancer therapy
Publication date: 30 July 2018 Source:International Journal of Pharmaceutics, Volume 546, Issues 1–2 Author(s): Jun Li, Huamin Liang, Jing Liu, Ziyuan Wang Poly (amidoamine) (PAMAM) dendrimers are well-defined, highly branched macromolecules with numerous active amine groups on the surface. Because of their unique properties, PAMAM dendrimers have steadily grown in popularity in drug delivery, gene therapy, medical imaging and diagnostic application. This review focuses on the recent developments on the application in PAMAM dendrimers as effective carriers for drug and gene (pDNA, siRNA) delivery in cancer therapy, incl...
Source: International Journal of Pharmaceutics - May 26, 2018 Category: Drugs & Pharmacology Source Type: research

Enhancing the therapeutic effect via elimination of hepatocellular carcinoma stem cells using Bmi1 siRNA delivered by cationic cisplatin Nanocapsules
Resistance of hepatocellular carcinoma (HCC) to systemic chemotherapy is partially due to presence of drug-resistant cancer stem cells. Bmi1 protein is essential for survival and proliferation of HCC cancer stem cells (CSCs). Here, we report that Bmi1 siRNA (Bmi1siR) loaded in cationic nanocapsules of cisplatin (NPC) eliminated stem cells in situ HCC in mice. NPC/Bmi1siR was fabricated via electrostatic complexation of Bmi1 siRNA to NPCs, which had cores composed of cisplatin and were coated with cationic lipids.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - May 26, 2018 Category: Nanotechnology Authors: Tan Yang, Yuyuan Chen, Pengxuan Zhao, Huiying Xue, Jia You, Bin Li, Yong Liu, Chuanchuan He, Xiaojuan Zhang, Lingling Fan, Robert J. Lee, Lei Li, Xiang Ma, Chuanrui Xu, Guangya Xiang Source Type: research

AMF siRNA treatment of keloid through inhibition signaling pathway of RhoA/ROCK1
In conclusion, AMF siRNA is expected to be a novel strategy to treat KD by inhibiting signaling pathway of RhoA/ROCK1.
Source: Genes and Diseases - May 19, 2018 Category: Genetics & Stem Cells Source Type: research

Molecular therapy using siRNA: Recent trends and advances of multi target inhibition of cancer growth.
Abstract RNA interference (RNAi) therapy, harnessed to produce a new class of drugs for treatment, has drawn attention and seen steady progress over the years. Molecular therapy using biological macromolecules small interfering RNA (siRNA) for gene silencing has received significant attention to target cancer-related genes. Basically, siRNA molecules bind to messenger RNAs (mRNA) by complementary base pairing, to induce degradation of the mRNA and/or block protein synthesis. Numerous genes and gene related proteins have been reported till date to target in siRNA based cancer therapy. Furthermore, a combination of ...
Source: International Journal of Biological Macromolecules - May 18, 2018 Category: Biochemistry Authors: Jain S, Pathak K, Vaidya A Tags: Int J Biol Macromol Source Type: research

GSE100403 A kinome-wide high-content siRNA screen identifies MEK5-ERK5 signaling as critical for breast cancer cell EMT and metastasis
Contributors : Simona Pavan ; Nathalie Meyer-Schaller ; Ravi K Kalathur ; Meera Saxena ; Gerhard ChristoforiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe have employed a high-content microscopy screen in combination with a kinome and phosphatome-wide siRNA library to identify signaling pathways underlying an EMT of murine mammary epithelial cells and breast cancer cells. This screen identified the MEK5-ERK5 axis as a critical player in TGFb-mediated EMT. Suppression of MEK5-ERK5 signaling completely prevented the morphological and molecular changes occurring during a TGFb-induced...
Source: GEO: Gene Expression Omnibus - May 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

Efficient siRNA transfer to knockdown a placenta specific lncRNA using RGD-modified nano-liposome: A new preeclampsia-like mouse model
Publication date: 30 July 2018 Source:International Journal of Pharmaceutics, Volume 546, Issues 1–2 Author(s): Qianwen Yu, Yue Qiu, Xuhui Wang, Jiajing Tang, Yayuan Liu, Ling Mei, Man Li, Ming Yang, Li Tang, Huile Gao, Zhirong Zhang, Wenming Xu, Qin He Preeclampsia is one of the most serious pregnancy complications. Many animal models have already been developed by researchers to study the pathogenesis and treatment of preeclampsia. However, most of these animal models were established by systemic administration or by surgery in the uterine cavity, which could lead to unwanted systemic toxicity or operative wounds and ...
Source: International Journal of Pharmaceutics - May 17, 2018 Category: Drugs & Pharmacology Source Type: research

Targeted delivery of CD44s-siRNA by ScFv overcomes de novo resistance to cetuximab in triple negative breast cancer
In this study, we show that impaired EGFR degradation is crucial for resistance to cetuximab, which depends on the cell surface molecule CD44. To further investigate the role of CD44 in EGFR signaling and its treatment potential, we developed a targeting fusion protein composed of an anti-EGFR scFv generated from cetuximab and truncated protamine, called Ce-tP. CD44 siRNA can be specifically delivered into EGFR-positive TNBC cells by Ce-tP. Efficient knockdown of CD44 and suppression of both EGFR and downstream signaling by the Ce-tP/siRNA complex were observed in EGFR-positive TNBC cells. More importantly, our results als...
Source: Molecular Immunology - May 17, 2018 Category: Allergy & Immunology Source Type: research

Tuned Density of Anti-Tissue Factor Antibody Fragment onto siRNA-loaded Polyion Complex Micelle for Optimizing Targetability into Pancreatic Cancer Cells.
Abstract Antibody fragment (Fab')-installed polyion complex (PIC) micelles were constructed to improve targetability of small interfering RNA (siRNA) delivery to pancreatic cancer cells. To this end, we synthesized a block copolymer of azide-functionalized poly(ethylene glycol) (PEG) and poly(L-lysine) (PLL), and prepared PIC micelles with siRNA. Then, a dibenzylcyclooctyne (DBCO)-modified anti-human tissue factor (TF) Fab' was conjugated to azido groups on the micellar surface. A fluorescence correlation spectroscopic (FCS) analysis revealed that 1, 2, or 3 molecule(s) of Fab'(s) were installed onto one micellar ...
Source: Biomacromolecules - May 16, 2018 Category: Biochemistry Authors: Min HS, Kim HJ, Ahn J, Naito M, Hayashi K, Toh K, Kim BS, Matsumura Y, Kwon IC, Miyata K, Kataoka K Tags: Biomacromolecules Source Type: research

TRIM24 siRNA induced cell apoptosis and reduced cell viability in human nasopharyngeal carcinoma cells.
Authors: Wang P, Shen N, Liu D, Ning X, Wu D, Huang X Abstract Nasopharyngeal carcinoma (NPC) is a common cancer occurring primarily in East Asia and Africa. The high rate of recurrence and metastasis of NPC continuously endangers the health of patients. The present study aimed to identify the underlying mechanisms involved in the progression of NPC and provide experimental basis to develop a novel and efficient agent against NPC. The present study measured the expression level of tripartite motif containing 24 (TRIM24) in tumor tissues from NPC patients using reverse transcription quantitative polymerase chain re...
Source: Molecular Medicine Reports - May 12, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Cellular uptake mechanism and comparative in vitro cytotoxicity studies of monomeric LMWP-siRNA conjugate
Publication date: 25 July 2018 Source:Journal of Industrial and Engineering Chemistry, Volume 63 Author(s): Junxiao Ye, Xing Pei, Hui Cui, Zhili Yu, Hyukjin Lee, Jianxin Wang, Xu Wang, Lu Sun, Huining He, Victor C. Yang The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical transla...
Source: Journal of Industrial and Engineering Chemistry - May 11, 2018 Category: Chemistry Source Type: research

The siRNA silencing of DcR3 expression induces Fas ligand-mediated apoptosis in HepG2 cells.
Authors: Zhao T, Xu Y, Ren S, Liang C, Zhou X, Wu J Abstract Dysfunctional Fas ligand (FasL) may inhibit the apoptosis of tumor cells. FasL contains two receptors, Fas and Decoy Receptor 3 (DcR3). DcR3 competitively binds to FasL over Fas, resulting in the inhibition of FasL-mediated apoptosis. Therefore, it was suggested that the downregulation of DcR3 expression enhances FasL-mediated apoptosis. In the current study, the expression of DcR3 was silenced in liver cancer HepG2 cells in order to study the effect of FasL on HepG2 cell activity and invasiveness. DcR3 siRNA knockdown HepG2 cells (KD), DcR3 blank plasmid...
Source: Experimental and Therapeutic Medicine - May 9, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Effective gene silencing activity of prodrug-type 2'-O-methyldithiomethyl siRNA compared with non-prodrug-type 2'-O-methyl siRNA.
Abstract Small interfering RNAs (siRNAs) are an active agent to induce gene silencing and they have been studied for becoming a biological and therapeutic tool. Various 2'-O-modified RNAs have been extensively studied to improve the nuclease resistance. However, the 2'-O-modified siRNA activities were often decreased by modification, since the bulky 2'-O-modifications inhibit to form a RNA-induced silencing complex (RISC). We developed novel prodrug-type 2'-O-methyldithiomethyl (MDTM) siRNA, which is converted into natural siRNA in an intracellular reducing environment. Prodrug-type 2'-O-MDTM siRNAs modified at th...
Source: Bioorganic and Medicinal Chemistry Letters - May 8, 2018 Category: Chemistry Authors: Hayashi J, Nishigaki M, Ochi Y, Wada SI, Wada F, Nakagawa O, Obika S, Harada-Shiba M, Urata H Tags: Bioorg Med Chem Lett Source Type: research

Effect of an siRNA Therapeutic Targeting PCSK9 on Atherogenic Lipoproteins: Pre-Specified Secondary End Points in ORION 1.
Conclusions -Inclisiran produces significant and prolonged reductions in atherogenic lipoproteins, suggesting that inhibiting synthesis of PCSK9 through siRNA may be a viable alternative to other approaches which target PCSK9. Clinical Trial Registration -URL: https://www.clinicaltrials.gov. Unique identifier: NCT02597127. PMID: 29735484 [PubMed - as supplied by publisher]
Source: Circulation - May 7, 2018 Category: Cardiology Authors: Ray KK, Stoekenbroek RM, Kallend D, Leiter LA, Landmesser U, Wright RS, Wijngaard P, Kastelein JJ Tags: Circulation Source Type: research

Ultrasound assisted gene and photodynamic synergistic therapy with multifunctional FOXA1-siRNA loaded porphyrin microbubbles for enhancing therapeutic efficacy for breast cancer.
Abstract To improve the non-invasive therapeutic efficacy for ER positive breast cancer (ER+ BC), we fabricated a multifunctional FOXA1 loaded porphyrin microbubble to combine photodynamic therapy (PDT) and gene therapy of FOXA1 knockdown (KD) with ultrasound targeted microbubble destruction (UTMD) technology under the guidance of contrast enhanced ultrasound (CEUS). Cationic porphyrin microbubbles (CpMBs) were firstly fabricated from a porphyrin grafted lipid with two cationic amino groups (PGL-NH2) and fluorocarbon inert gas of C3F8. Porphyrin group in the CpMBs monolayer could be used as a photosensitizer for ...
Source: Biomaterials - May 3, 2018 Category: Materials Science Authors: Zhao R, Liang X, Zhao B, Chen M, Liu R, Sun S, Yue X, Wang S Tags: Biomaterials Source Type: research

Cell-penetrating peptides for siRNA delivery to glioblastomas
In conclusion, we have established an efficient non-covalently complexed carrier (PF14:TG1) for siRNA delivery to human glioblastoma cells (U87), showing a significant two-fold increase in gene-silencing efficiency compared to the parent peptide PF14 and also improved specificity to U87 cells compared to non-glioma targeted cells. Graphical abstract
Source: Peptides - May 1, 2018 Category: Biochemistry Source Type: research

A kinome-wide high-content siRNA screen identifies MEK5–ERK5 signaling as critical for breast cancer cell EMT and metastasis
A kinome-wide high-content siRNA screen identifies MEK5–ERK5 signaling as critical for breast cancer cell EMT and metastasis, Published online: 01 May 2018; doi:10.1038/s41388-018-0270-8A kinome-wide high-content siRNA screen identifies MEK5–ERK5 signaling as critical for breast cancer cell EMT and metastasis
Source: Oncogene - April 30, 2018 Category: Cancer & Oncology Authors: Simona Pavan Nathalie Meyer-Schaller Maren Diepenbruck Ravi Kiran Reddy Kalathur Meera Saxena Gerhard Christofori Source Type: research

Potential of siRNA-albumin complex against cancer.
Abstract RNA interference is a highly specific as well as efficient technology for gene therapy application in molecular oncology. The present study was planned to develop an efficient and stable tumor selective delivery mechanism for siRNA gene therapy for the purpose of both diagnosis as well as therapy. We have used 20 Male wistar rats for the formation of colon cancer model and utilized albumin as carrier molecule for the delivery of siRNA against vascular endothelial growth factor receptor 2 (VEGF R2). The study results confirmed efficient delivery of siRNA at tumor site as confirmed by tagging of siRNA-album...
Source: Chemico-Biological Interactions - April 27, 2018 Category: Molecular Biology Authors: Liu N, Qi YH, Cheng CT, Yang WB, Malhotra A, Zhou Q Tags: Chem Biol Interact Source Type: research

Co-Delivery of Doxorubicin and Anti-BCL-2 siRNA by pH-Responsive Polymeric Vector to Overcome Drug Resistance in In Vitro and In Vivo HepG2 Hepatoma Model.
In this study, triblock copolymer of poly(ethylene glycol)-block-poly(L-lysine)-block-poly aspartyl (N-(N',N'-diisopropylaminoethyl)) (PEG-PLL-PAsp(DIP)) was synthesized for the first time to enable the codelivery of BCL-2 siRNA and DOX. The system is supposed to only bypass drug efflux but also down-regulate the anti-apoptotic gene and consequently confronting against chemoresistance as well. Moreover, the pH responsive ability of the codelivery system can prevent drug leakage during circulation and guarantee swift release at tumors. The codelivered siRNA serves to suppress the expression of anti-apoptotic BCL-2 and conse...
Source: Biomacromolecules - April 24, 2018 Category: Biochemistry Authors: Sun W, Chen X, Xie C, Wang Y, Lin L, Zhu K, Shuai X Tags: Biomacromolecules Source Type: research