Query: siRNA

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Total 14644 results found since Jan 2013.

Characterization and function of the T-box 1 gene in Chinese giant salamander Andrias davidianus.
Abstract We characterized the Andrias davidianus T-box 1 (Tbx1) gene. Tbx1 expression was high in testis and low in other examined tissues. Immunohistochemistry detected tbx1 expression in somatic and germ cells 62 days post-hatching (dph), prior to gonad differentiation. At 210 dph, after gonad differentiation, tbx1 was expressed in spermatogonia and testis somatic cells and in granulosa cells in ovary. Tbx1 expression was up-regulated in ovary after high temperature treatment. In the neomale, tbx1 expression showed a similar profile to normal males, and vice-versa for genetic male. Over-expression of tbx1 in f...
Source: Genomics - September 20, 2018 Category: Genetics & Stem Cells Authors: Hu Q, Meng Y, Wang D, Tian H, Xiao H Tags: Genomics Source Type: research

Apoptotic cell death induced by Z-Ligustilidein human ovarian cancer cells and role of NRF2.
Abstract Z-Ligustilide is the most potent bioactive component of Angelica sinensis, which is widely used in Chinese traditional medicine. Z-Ligustilide selectively affected ovarian cancer cell survival in a dose dependent manner. Z-Ligustilide induced apoptotic cell death was determined by flow cytometry. We also demonstrated that apoptotic cell death was triggered by Z-Ligustilideinduced oxidative stress and mitochondria played an active role. Mitochondrial polarization was reduced by Z-Ligustilidewhereas mitochondrial superoxide formation was increased. NRF2 was induced by Z-Ligustilide in OVCAR-3 cells at epi...
Source: Food and Chemical Toxicology - September 20, 2018 Category: Food Science Authors: Lang F, Qu J, Yin H, Li L, Zhi Y, Liu Y, Fang Z, Hao E Tags: Food Chem Toxicol Source Type: research

LncRNA HOTAIR mediates TGF- β2-induced cell growth and epithelial-mesenchymal transition in human lens epithelial cells.
In this study, we confirmed that TGF-β2 induces EMT in SRA01/04 cells via the up-regulation of the long non-coding RNA (lncRNA) HOTAIR. To study the effects of HOTAIR on the proliferation, migration and EMT of SRA01/04 cells as well as the underlying mechanism, we used small interfering RNA (siRNA) to specifically attenuate HOTAIR expression in SRA01/04 cells. CCK8 cell-counting kit was used to examine SRA01/04 cell viability; EdU cell proliferation kit was used to examine SRA01/04 cell proliferation; Transwell system and scratch assays were used to observe cell migration; and qPCR and western blot analysis were used to e...
Source: Acta Biochimica et Biophysica Sinica - September 20, 2018 Category: Biochemistry Authors: Zhang Z, Zhu H, Liu Y, Quan F, Zhang X, Yu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Perturbations of Neuron-Restrictive Silencing Factor Modulate Corticotropin-Releasing Hormone Gene Expression in the Human Cell Line BeWo
Stress exacerbates disease, and understanding its molecular mechanisms is crucial to the development of novel therapeutic interventions to combat stress-related disorders. The driver of the stress response in the hypothalamic-pituitary-adrenal axis (HPA) is corticotropin-releasing hormone (CRH), a neuropeptide synthesized in the paraventricular nucleus of the hypothalamus. Evidence supports that CRH expression is epigenetically modified at the molecular level by environmental stimuli, causing changes in the stress response. This effect is mediated by a concert of factors that translate environmental change into alterations...
Source: Molecular Neuropsychiatry - September 19, 2018 Category: Neuroscience Source Type: research

Capn4 expression is modulated by microRNA-520b and exerts an oncogenic role in prostate cancer cells by promoting Wnt/β-catenin signaling
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Wei Ren, Dong Wang, Chan Li, Tao Shu, Wei Zhang, Xiaoliang FuAbstractAccumulating evidence reports that Capn4 plays an important role in the development and progression of various malignant cancers. However, whether Capn4 is involved in prostate cancer remains unclear. Therefore, the aim of this study was to investigate the expression, biological function and regulatory mechanism of Capn4 in prostate cancer. Herein, we found that Capn4 was highly expressed in prostate cancer cell lines compared with normal prostate cells. Capn4 gene ...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

c-Fos/microRNA-18a feedback loop modulates the tumor growth via HMBOX1 in human gliomas
In conclusion, our study determine that c-Fos/miR-18a feedback loop promotes the tumor growth of gliomas by HMBOX1, providing important clues for understanding the key roles of transcription factor mediated mRNA-miRNA functional network in the regulation of gliomas.Graphical abstract
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

DIXDC1 promotes the growth of acute myeloid leukemia cells by upregulating the Wnt/β-catenin signaling pathway
In this study, we aimed to investigate the expression status and potential biological function of DIXDC1 in AML. Our results showed that DIXDC1 expression was highly upregulated in AML cell lines and primary AML blasts compared with normal blasts. Knockdown of DIXDC1 by siRNA-mediated gene silencing significantly inhibited proliferation, induced cell cycle arrest, and promoted apoptosis of AML cells in vitro. By contrast, DIXDC1 overexpression promoted proliferation, accelerated cell cycle progression, and reduced apoptosis of AML cells. Moreover, we found that DIXDC1 knockdown decreased the expression of β-catenin and re...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

The silencing of ApoC3 suppresses oxidative stress and inflammatory responses in placenta cells from mice with preeclampsia via inhibition of the NF-κB signaling pathway
ConclusionSilencing ApoC3 could suppress the NF-κB signaling pathway, thereby exercising a protective effect on cell injury induced by oxidative stress and reducing inflammatory responses.Graphical abstractThe molecular mechanism involving silenced APOC3 regulating the oxidative stress and inflammatory responses in placenta cells of preeclampsia mice via inhibition of the NF-κB signaling pathway. In preeclampsia mice, up-regulated APOC3 increased the expression of p65 and inhibited the expression of phosphorylation of IkBα, thus resulting in the oxidative stress injury and inflammatory responses of placenta cells. While...
Source: Biomedicine and Pharmacotherapy - September 19, 2018 Category: Drugs & Pharmacology Source Type: research

Green tea compound helps siRNA slip inside cells
(American Chemical Society) Drinking green tea has been linked to health benefits ranging from cardiovascular disease prevention to weight loss. Although many of these claims still need to be verified in the clinic, an antioxidant in green tea called epigallocatechin gallate (EGCG) appears to have beneficial effects in cells and animals. Now, researchers have found a surprising use for EGCG: sneaking therapeutic RNAs into cells. They report their results in ACS Central Science.
Source: EurekAlert! - Medicine and Health - September 19, 2018 Category: International Medicine & Public Health Source Type: news

Palmitate aggravates proteinuria-induced cell death and inflammation via CD36-inflammasome axis in the proximal tubular cells of obese mice.
In this study, we investigated the roles of CD36 and NLRP3 inflammasomes in FFA-induced renal injury in high-fat diet (HFD)-induced obesity. HFD-fed C57BL/6 mice and palmitate-treated HK2 renal tubular cells were used as in vivo and in vitro models. Immunohistochemical staining showed that CD36, IL-1β, and IL-18 levels increased progressively in the kidneys of HFD-fed mice. Sulfo-N-succinimidyl oleate (SSO), a CD36 inhibitor, attenuated the HFD-induced upregulation of NLRP3, IL-1β, and IL-18 and suppressed the colocalization of NLRP3 and ASC in renal tubular cells. In vitro, SSO abolished the palmitate-induced activation...
Source: American Journal of Physiology. Renal Physiology - September 19, 2018 Category: Physiology Authors: Li LC, Yang JL, Lee WC, Chen JB, Lee CT, Wang PW, Vaghese Z, Chen WY Tags: Am J Physiol Renal Physiol Source Type: research

LncRNA-135528 inhibits tumor progression by up-regulating CXCL10 through the JAK/STAT pathway
AbstractSpontaneous tumor regression can be observed in many tumors, however, studies related to the altered expression of lncRNA in spontaneous glioma regression are limited, and the potential contributions of lncRNAs to spontaneous glioma regression remain unknown. To investigate the biological roles of lncRNA-135528 in spontaneous glioma regression. The cDNA fragment of lncRNA-135528 was obtained by rapid-amplification of cDNA ends (RACE) technology and cloned into the plvx-mcmv-zsgreen-puro vector. Additionally, we stably silenced or overexpressed lncRNA-135528 in G422 cells by transfecting with siRNA against lncRNA-13...
Source: Apoptosis - September 19, 2018 Category: Molecular Biology Source Type: research

Fibroblast growth factor 19 improves cardiac function and mitochondrial energy homoeostasis in the diabetic heart.
In this study, we investigated the effects of FGF19 on energy metabolism. FGF19 treatment of diabetic hearts exhibited higher glucose uptake and lower lipid profiles, suggesting changes in energy metabolism. The protective effects of FGF19 prevented ventricular dysfunction in diabetic hearts and improved mitochondrial function by the upregulation of PGC-1α expression. On the other side, knockdown of PGC-1α by siRNA attenuated the effects of FGF19 on the enhancement of mitochondrial function and energy efficiency. Taken together, these results show that FGF19 exhibited improved mitochondrial efficiency, which might be ass...
Source: Biochemical and Biophysical Research communications - September 19, 2018 Category: Biochemistry Authors: Xu W, Wang Y, Guo Y, Liu J, Ma L, Cao W, Yu B, Zhou Y Tags: Biochem Biophys Res Commun Source Type: research

A highly selective fluorescent probe for detecting glutathione transferases to reveal anticancer-activity sensitivity of cisplatin in cancer cells and tumor tissues
Publication date: 20 December 2018Source: Sensors and Actuators B: Chemical, Volume 277Author(s): Xiangge Tian, Zhenhao Tian, Yan Wang, Jie Hou, Lei Feng, Liang Song, Jingnan Cui, Xiaokui Huo, Xinyu Zhao, Dayong Zhou, Chao Wang, Chengpeng Sun, Jing Ning, Xiaochi MaAbstractCisplatin is a widely used anti-cancer drug in clinic, while many cancer cells gradually exhibited serious cisplatin resistance recently, which resulted in reducing its clinical effects. Glutathione transferases (GSTs) as an extensive expressed metabolic enzyme in human, plays a significant role in the metabolism of cisplatin. Thus, sensitively and accura...
Source: Sensors and Actuators B: Chemical - September 18, 2018 Category: Chemistry Source Type: research

Inhibition of endoplasmic reticulum stress by intermedin1-53 attenuates angiotensin II –induced abdominal aortic aneurysm in ApoE KO Mice
In conclusion, IMD may protect against AAA formation by inhibiting ERS via activating AMPK phosphorylation.
Source: Endocrine - September 18, 2018 Category: Endocrinology Source Type: research

The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease
Regulatory T cells (Tregs) are critical for maintaining immune homeostasis. However, current Treg immunotherapies do not optimally treat inflammatory diseases in patients. Understanding the cellular processes that control Treg function may allow for the augmentation of therapeutic efficacy. In contrast to activated conventional T cells, in which protein kinase C-θ (PKC-θ) localizes to the contact point between T cells and antigen-presenting cells, in human and mouse Tregs, PKC-θ localizes to the opposite end of the cell in the distal pole complex (DPC). Here, using a phosphoproteomic screen, we identified the intermedia...
Source: Journal of Clinical Investigation - September 18, 2018 Category: Biomedical Science Authors: Cameron McDonald-Hyman, James T. Muller, Michael Loschi, Govindarajan Thangavelu, Asim Saha, Sudha Kumari, Dawn K. Reichenbach, Michelle J. Smith, Guoan Zhang, Brent H. Koehn, Jiqiang Lin, Jason S. Mitchell, Brian T. Fife, Angela Panoskaltsis-Mortari, Col Source Type: research

Silencing of Annexin A1 suppressed the apoptosis and inflammatory response of preeclampsia rat trophoblasts.
Abstract Preeclampsia (PE) is a disorder that is characterized by pregnancy‑induced hypertension. It has been reported that Annexin A1 (ANXA1) is highly expressed in the plasma of women diagnosed with PE. Therefore, the present study aimed to examine the effect of ANXA1 on PE rats. The PE animal model was constructed in rats using Nω‑nitro‑L‑arginine methyl ester (L‑NAME), and the blood pressure and urine protein levels of rats were detected. The pathological features of placental tissue, and the levels of inflammatory factors and ANXA1 were respectively measured by hematoxylin‑eosin staining, enzyme...
Source: International Journal of Molecular Medicine - September 18, 2018 Category: Molecular Biology Authors: Feng J, Wang X, Li H, Wang L, Tang Z Tags: Int J Mol Med Source Type: research

DNMT1, a Novel Regulator Mediating mTORC1/mTORC2 Pathway-Induced NGF Expression in Schwann Cells.
Abstract Schwann cells play an important role in maintaining the normal function of peripheral nerves via the secretion of nerve growth factor (NGF). The mTOR signaling pathway is known as a kind of Ser/Thr protein kinase that regulates various cell functions. DNA methyltransferase 1 (DNMT1) is an epigenetic regulator and downstream target of the mTOR pathway. In the present study, we explored the relationship between NGF expression and the mTOR pathway/DNMT1 in RSC96 cells. The results showed that both rapamycin and Torin 1 downregulated NGF expression via the inhibition of phospho-mTOR (Ser 2448) and phospho-S6K...
Source: Neurochemical Research - September 18, 2018 Category: Neuroscience Authors: Cheng M, Lv X, Zhang C, Du W, Liu Y, Zhu L, Hao J Tags: Neurochem Res Source Type: research

Decreased expression of LATS1 correlates with astrogliosis after spinal cord injury.
Abstract Large tumor suppressor kinase 1 (LATS1) is a serine/threonine kinase of the AGC kinase family in mammals and involved in various biological processes, it is a key regulator of cell cycle progression. However, the role of LATS1 in central nervous system trauma is still unknown. In present study, we performed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of LATS1 expression in the spinal cord. We found that LATS1 protein levels were significantly decreased at day 1 after injury. Meanwhile, double immunofluorescence staining showed these changes were striking in a...
Source: Biochemical and Biophysical Research communications - September 18, 2018 Category: Biochemistry Authors: Wang Y, Chen M Tags: Biochem Biophys Res Commun Source Type: research

CDC25B promotes influenza A virus replication by regulating the phosphorylation of nucleoprotein.
Abstract Cell division cycle 25 B (CDC25B) is a member of the CDC25 phosphatase family. It can dephosphorylate cyclin-dependent kinases and regulate the cell division cycle. Moreover, siRNA knockdown of CDC25B impairs influenza A virus (IAV) replication. Here, to further understand the regulatory mechanism of CDC25B for IAV replication, a CDC25B-knockout (KO) 293T cell line was constructed using CRISPR/Cas9. The present data indicated that the replication of IAV was decreased in CDC25B-KO cells. Additionally, CDC25B deficiency damaged viral polymerase activity, nucleoprotein (NP) self-oligomerization, and NP nucle...
Source: Virology - September 18, 2018 Category: Virology Authors: Cui L, Mahesutihan M, Zheng W, Meng L, Fan W, Li J, Ye X, Liu W, Sun L Tags: Virology Source Type: research

Phosphodiesterase 4 inhibitor activates AMPK-SIRT6 pathway to prevent aging-related adipose deposition induced by metabolic disorder.
Abstract Rolipram is a selective phosphodiesterase 4 (PDE4) inhibitor that exerts a variety of effects, including anti-inflammatory, immunosuppressive, and anti-tumor effects. The aim of this study was to investigate the effect of rolipram on metabolic disorder and its underlying mechanisms. Metabolic disorder was induced in 8-week-old wild type BABL/c mice by administration of D-galactose for 4 weeks. Simultaneously the mice were administered vehicle or rolipram. Alternatively, beginning at 3 or 21 months, the mice were administered db-cAMP for 3 months, with or without a high-fat-diet (HFD) to induce metabolic d...
Source: Aging - September 18, 2018 Category: Biomedical Science Authors: Wang Z, Liang Y, Zhang L, Zhang N, Liu Q, Wang Z Tags: Aging (Albany NY) Source Type: research

Proliferation Potential-Related Protein Promotes the Esophageal Cancer Cell Proliferation, Migration and Suppresses Apoptosis by Mediating the Expression of p53 and Interleukin-17
Conclusion: Our data suggest that PP-RP promotes esophageal cancer cell proliferation and migration, and suppresses apoptosis by mediating the expression of p53 and IL-17.Pathobiology
Source: Pathobiology - September 17, 2018 Category: Pathology Source Type: research

ASAP Charge and Assembly Reversible Micelles Fueled by Intracellular ATP for Improved siRNA Transfection
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b13300
Source: ACS Applied Materials and Interfaces - September 17, 2018 Category: Materials Science Authors: Zhanwei Zhou, Chenzi Li, Minghua Zhang, Qingyan Zhang, Chenggen Qian, David Oupicky, Minjie Sun Source Type: research

Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II
AbstractCaliciviruses in the genusSapovirus are a significant cause of viral gastroenteritis in humans and animals. However, the mechanism of their entry into cells is not well characterized. Here, we determined the entry mechanism of porcine sapovirus (PSaV) strain Cowden into permissive LLC-PK cells. The inhibition of clathrin-mediated endocytosis using chlorpromazine, siRNAs, and a dominant negative (DN) mutant blocked entry and infection of PSaV Cowden strain, confirming a role for clathrin-mediated internalization. Entry and infection were also inhibited by the cholesterol-sequestering drug methyl- β-cyclodextrin and...
Source: Veterinary Research - September 17, 2018 Category: Veterinary Research Source Type: research

Adenine Nucleotide Translocase 2 as an Enzyme Related to 18 F FDG Accumulation in Various Cancers
ConclusionIn various cancer cells and tissues, the expression levels of ANT2 explained [18F] FDG accumulation better than those of GLUT1 and HK2. ANT2 can be used as a marker of dedifferentiated pathology and aggressiveness of cancer.
Source: Molecular Imaging and Biology - September 17, 2018 Category: Molecular Biology Source Type: research

MicroRNA-338-5p plays a tumor suppressor role in glioma through inhibition of the MAPK-signaling pathway by binding to FOXD1
ConclusionWe found that overexpression of miR-338-5p suppresses glioma cell proliferation, migration, and invasion and accelerates its senescence and apoptosis by decreasing FOXD1 expression via inhibition of activation of MAPK-signaling pathway.
Source: Journal of Cancer Research and Clinical Oncology - September 17, 2018 Category: Cancer & Oncology Source Type: research

ZIP9 but not the androgen receptor mediates testosterone-induced migratory activity of metastatic prostate cancer cells
Publication date: Available online 15 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Ahmed Bulldan, Joerg-Walter Bartsch, Lutz Konrad, Georgios Scheiner-BobisAbstractLNCaP cells are derived from a metastatic lesion of human prostate adenocarcinoma. They express the classical androgen receptor (AR) and ZIP9, a Zn2+ transporter that also binds testosterone and mediates signaling by interacting with G-proteins.Our results show that LNCaP cells respond to testosterone by mobilizing their migratory machinery. Their exposure to testosterone triggers the formation of lamellipodia, re...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 16, 2018 Category: Molecular Biology Source Type: research

Gefitinib induces non-small cell lung cancer H1650 cell apoptosis through downregulating tumor necrosis factor-related apoptosis-inducing ligand expression levels.
Authors: Yi H, Li S, Li H, Wang P, Zheng H, Cheng X Abstract Non-small cell lung cancer (NSCLC) presents severe threats to the lives of patients. Gefitinib is one of the first-line drugs available for the treatment of NSCLC in the clinical setting. The present study investigated the effects of gefitinib on NSCLC H1650 cell viability and apoptosis via MTT assays and flow cytometry. Western blot analysis was employed to detect tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression levels in H1650 cells. In the present study, H1650 cells were treated with TRAIL siRNA or an empty plasmid vector cont...
Source: Oncology Letters - September 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Long non-coding RNA-CCAT2 promotes the occurrence of non-small cell lung cancer by regulating the Wnt/ β-catenin signaling pathway.
Long non-coding RNA-CCAT2 promotes the occurrence of non-small cell lung cancer by regulating the Wnt/β-catenin signaling pathway. Oncol Lett. 2018 Oct;16(4):4600-4606 Authors: Zhao C, Qiao C, Zong L, Chen Y Abstract The present study aimed to investigate the biological function of colon cancer-associated transcript 2 (CCAT2) in the occurrence and progression of non-small cell lung carcinoma (NSCLC) and its potential use in the early diagnosis and molecular-targeted therapy of NSCLC. The tumor tissues, para-carcinoma tissues and associated clinical data of 36 patients with NSCLC were collected in orde...
Source: Oncology Letters - September 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Downregulation of peroxiredoxin II suppresses the proliferation and metastasis of gastric cancer cells.
Authors: Niu L, Liu A, Xu W, Yang L, Zhu W, Gu Y Abstract Peroxiredoxin (Prx) II is an imperative member of the superfamily of peroxidases. It serves an essential role in scavenging organic hydroperoxide and H2O2. It is involved in the development of various malignant tumors. In order to investigate the significance of Prx II expressions level in gastric cancer (GC), downregulation of Prx II was performed to investigate its role in the proliferation and migration of gastric adenocarcinoma cells. In GC cells and 45 GC specimens, the mRNA and protein expression levels of Prx II were determined using reverse transcrip...
Source: Oncology Letters - September 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

High serum Ephrin-B2 levels predict poor prognosis for patients with gastric cancer.
Authors: Yue WJ, Liu YP, Li M, Liu CX, Mou SJ, Li QK, Chen ZP Abstract Gastric cancer is an intractable disease with a poor prognosis and limited treatment options. Its treatment remains a major clinical challenge worldwide. Ephrin-B2 is upregulated and involved in tumor growth in various types of cancer. However, the association between ephrin-B2 and prognosis of gastric cancer, and the potential of ephrin-B2 as a therapeutic target remains unknown. The present study investigated ephrin-B2 as a prognostic factor and a therapeutic target for gastric cancer. Reverse transcription-quantitative polymerase chain reacti...
Source: Oncology Letters - September 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effects of RSF-1 on proliferation and apoptosis of breast cancer cells.
Authors: Liu Y, Gai J, Fu L, Zhang X, Wang E, Li Q Abstract Effect of interference with chromatin remodeling and spacing factor-1 (RSF-1) on proliferation and apoptosis of breast cancer cells was investigated. MCF-7 and SKBR-3 cells were cultured in vitro and were divided into 3 groups: control group, negative siRNA control group (NC) and RSF-1 siRNA group. Western blot analysis was used to detect the expression of RSF protein after interference. Cell Counting Kit-8 (CCK-8) method was used to detect the effect of RSF-1 siRNA on cell proliferation. Plate cloning assay was used to detect the effect of RSF-1 siRNA on ...
Source: Oncology Letters - September 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Activation of Estrogen Receptor Alpha Induces Beiging of Adipocytes
ConclusionOur results demonstrate for the first time that selective-activation of ERα in adipocytes increases markers of beiging and this is likely through induction of AMPK and ATGL-mediated lipolysis providing FFAs as a fuel to activate UCP-1.
Source: Molecular Metabolism - September 16, 2018 Category: Endocrinology Source Type: research

Heme oxygenase-1 inhibits neointimal hyperplasia in rat by histone deacetylase 2.
Authors: Ni J, Yang W, Shen W, Zhang R Abstract It has been reported that heme oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this induction was attenuated after the treatment of siRNA against Hmox1. Interestingly, this HDAC2 induction was dependent on Hmox1 protein as well as its enzymatic activity, and was regulated by carbon monoxide released from heme degradation. Furthermore, hist...
Source: Free Radical Research - September 16, 2018 Category: Research Tags: Free Radic Res Source Type: research

GSE73092 High-throughput RNAi cell viability screen to identify selective targets for EWS-FLI1 positive Ewing sarcoma
Contributors : Kristiina Iljin ; Tao HeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe have performed a high-throughput RNA interference screen to identify targets inhibiting EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 expressing A673 Ewing sarcoma cells were screened both in presence and absence of EWS-FLI1 shRNA induction with druggable siRNA library. Leucine rich repeats and WD repeat Domain containing 1 (LRWD1) targeting siRNA pool was the strongest anti-proliferative hit identified only in presence of EWS-FLI1. Validation experiments confirmed the anti-...
Source: GEO: Gene Expression Omnibus - September 16, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Novel Nano therapeutic materials for the effective treatment of Rheumatoid Arthritis- recent insights
Publication date: Available online 15 September 2018Source: Materials Today CommunicationsAuthor(s): Kumar Janakiraman, Venkateshwaran Krishnaswami, Vijaya Rajendran, Subramanian Natesan, Ruckmani KandasamyAbstractRheumatoid arthritis (RA) is the most common complex multifactorial joint related autoimmune inflammatory disease with unknown etiology accomplished with increased cardiovascular risks. RA is characterized by the clinical findings of synovial inflammation, autoantibody production, and cartilage/bone destruction, cardiovascular, pulmonary and skeletal disorders. Pro-inflammatory cytokines such as IL-1, IL-6, IL-8,...
Source: Materials Today Communications - September 15, 2018 Category: Materials Science Source Type: research

Sphingosine kinase 2 cooperating with Fyn promotes kidney fibroblast activation and fibrosis via STAT3 and AKT
Publication date: Available online 15 September 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseAuthor(s): Xingxing Zhu, Dongyan Shi, Kelei Cao, Dongqing Ru, Jiafa Ren, Zebing Rao, Yunzi Chen, Qiang You, Chunsun Dai, Lixin Liu, Hong ZhouAbstractSphingosine kinases (Sphks) are the rate-limiting enzymes in the conversion of sphingosine to biologically active sphingosine-1-phosphate. The present study aimed to determine the role of Sphk2 and its downstream targets in renal fibroblast activation and interstitial fibrosis. In the kidney interstitium of patients with renal fibrosis, Sphk2high-expressi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - September 15, 2018 Category: Molecular Biology Source Type: research

Melatonin protects against myocardial ischemia-reperfusion injury by elevating Sirtuin3 expression and manganese superoxide dismutase activity.
Authors: Feng J, Chen X, Liu R, Cao C, Zhang W, Zhao Y, Nie S Abstract Myocardial ischemia-reperfusion (MI/R) injury is a crucial cause for mortality throughout the world. Recent studies indicated that melatonin might exert profound cardio-protective effect in MI/R injury. However, the underlying mechanisms are not completely understood. In the current study, we aimed to explore the potential effect of melatonin in the pathological process of MI/R. Both in vivo MI/R model and in vitro H9c2 cell line simulated I/R (SIR) model were applied with or without melatonin supplementation. We found that Sirtuin3 (Sirt3) expr...
Source: Free Radical Research - September 15, 2018 Category: Research Tags: Free Radic Res Source Type: research

Cell-free DNA derived from cancer cells facilitates tumor malignancy through Toll-like receptor 9 signaling-triggered interleukin-8 secretion in colorectal cancer.
Abstract Circulating cell-free DNA (cfDNA) has become a potential diagnostic and prognostic biomarker for colorectal cancer (CRC). In non-cancerous diseases, it has been confirmed that cfDNA can be recognized by Toll-like receptor 9 (TLR9), leading to a significant biological change. Nevertheless, the biological significance of cfDNA and its relationship with TLR9 in tumor malignancy is still unclear. Therefore, the purpose of this study is to explore the biological role of cfDNA in colorectal cancer (CRC). The expression of TLR9 was measured in different CRC cell lines and cancerous samples by RT-PCR or immunohis...
Source: Acta Biochimica et Biophysica Sinica - September 15, 2018 Category: Biochemistry Authors: Niu Z, Tang W, Liu T, Xu P, Zhu D, Ji M, Huang W, Ren L, Wei Y, Xu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Silencing of PMEL attenuates melanization via activating lysosomes and degradation of tyrosinase by lysosomes.
Abstract The functionally specialized melanosome is a membrane-enclosed lysosome-related organelle, which coexists with lysosomes in melanocytes. Pre-melanosomal protein (PMEL) initiates pre-melanosome morphogenesis and is the only cell-specific pigment protein required for the formation of fibrils on which melanin is deposited in melanosomes. But the effects of PMEL on melanin synthesis and lysosome activity remain unclear. In the study, PMEL was silenced in human epidermal melanocytes by siRNA transfection. Compared to the non-treated group, melanin content in the transfected cells was greatly reduced. Real-time...
Source: Biochemical and Biophysical Research communications - September 14, 2018 Category: Biochemistry Authors: Sun L, Hu L, Zhang P, Li H, Sun J, Wang H, Xie X, Hu J Tags: Biochem Biophys Res Commun Source Type: research

GSE109717 Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae
Contributors : Shilpi Chandra ; James Gray ; William B Kiosses ; Archana Khurana ; Kaori Hitomi ; Catherine M Crosby ; Ashu Chawla ; Zheng Fu ; Meng Zhao ; Natacha Veerapen ; Stewart K Richardson ; Steven A Porcelli ; Gurdyal Besra ; Amy R Howell ; Sonia Sharma ; Bjoern Peters ; Mitchell KronenbergSeries Type : Expression profiling by arrayOrganism : Mus musculusInvariant natural killer T cells (iNKT cells) are activated by lipid antigens presented by CD1d, but the pathway leading to lipid antigen presentation remains incompletely characterized. Here we show a whole genome siRNA screen to elucidate the CD1d presentation pa...
Source: GEO: Gene Expression Omnibus - September 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC.
In this study, VAOS exhibited effective inhibitory effects in NSCLC both in cultured cells and in a xenograft mouse model. VAOS was further identified to induce NSCLC cell apoptosis through activating protein kinase B (AKT) to elevate intracellular reactive oxygen species (ROS) levels by increasing in oxygen consumption and impairing the ROS-scavenging system. Neither silencing of PTP1B by siRNA nor transient overexpression of PTP1B had an effect on the AKT phosphorylation triggered by VAOS, indicating that PTP1B inhibition was not involved in VAOS-induced apoptosis. Through phosphorus colorimetric assay, we demonstrated t...
Source: Free Radical Biology and Medicine - September 14, 2018 Category: Biology Authors: Zhou L, Yi Y, Yuan Q, Zhang J, Li Y, Wang P, Xu M, Xie S Tags: Free Radic Biol Med Source Type: research

TRPM7 channels play a role in high glucose-induced endoplasmic reticulum stress and neuronal cell apoptosis Signal Transduction
High-glucose (HG) levels and hyperglycemia associated with diabetes are known to cause neuronal damage. The detailed molecular mechanisms, however, remain to be elucidated. Here, we investigated the role of transient receptor potential melastatin 7 (TRPM7) channels in HG-mediated endoplasmic reticulum stress (ERS) and injury of NS20Y neuronal cells. The cells were incubated in the absence or presence of HG for 48 h. We found that mRNA and protein levels of TRPM7 and of ERS-associated proteins, such as C/EBP homologous protein (CHOP), 78-kDa glucose-regulated protein (GRP78), and inducible nitric-oxide synthase (iNOS), incr...
Source: Journal of Biological Chemistry - September 14, 2018 Category: Chemistry Authors: Yan Huang, Tian-Dong Leng, Koichi Inoue, Tao Yang, Mingli Liu, F. David Horgen, Andrea Fleig, Jun Li, Zhi-Gang Xiong Tags: Molecular Bases of Disease Source Type: research

Plant mRNA decay: extended roles and potential determinants.
Abstract The decay of mRNA in plants is tightly controlled and shapes the transcriptome. The roles of this process are to digest RNA as well as to suppress exogenous and endogenous gene silencing by preventing siRNA generation. Recent evidence suggests that mRNA decay also regulates the accumulation of the putative 3' fragment-derived long non-coding RNAs (3'lncRNAs). The generation of siRNA or 3'lncRNA from a selective subset of mRNAs raises a fundamental question of how the mRNA decay machineries select and determine their substrate transcripts for distinctive decay destiny. Evidence for potential mRNA decay det...
Source: Current Opinion in Plant Biology - September 14, 2018 Category: Biology Authors: Li B, Wu H, Guo H Tags: Curr Opin Plant Biol Source Type: research

Dual-layer transposon repression in heads of Drosophila melanogaster.
Abstract piRNA-mediated repression of transposable elements (TE) in the germline limits the accumulation of heritable mutations caused by their transposition in the genome. It is not clear whether the piRNA pathway plays a functional role in adult, non-gonadal tissues in Drosophila melanogaster. To address this question, we first analyzed the small RNA content of adult Drosophila melanogaster heads. We found that varying amount of piRNA-sized, ping-pong positive molecules in heads correlates with contamination by gonadal tissue during RNA extraction, suggesting that most of piRNAs detected in head sequencing libra...
Source: RNA - September 14, 2018 Category: Genetics & Stem Cells Authors: van den Beek M, da Silva B, Pouch J, Ali Chaouche MEA, Carré C, Antoniewski C Tags: RNA Source Type: research

Hispidulin induces ER stress-mediated apoptosis in human hepatocellular carcinoma cells in vitro and in vivo by activating AMPK signaling pathway.
This study provides new insights into the anti-tumor activity of hispidulin in HCC. PMID: 30218072 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - September 14, 2018 Category: Drugs & Pharmacology Authors: Han M, Gao H, Xie J, Yuan YP, Yuan Q, Gao MQ, Liu KL, Chen XH, Han YT, Han ZW Tags: Acta Pharmacol Sin Source Type: research

Thyroid hormone (T3) stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy.
Abstract The thyroid hormone triiodothyronine (T3) activates thermogenesis by uncoupling electron transport from ATP synthesis in brown adipose tissue (BAT) mitochondria. Although T3 can induce thermogenesis by sympathetic innervation, little is known about its cell autonomous effects on BAT mitochondria. We thus examined effects of T3 on mitochondrial activity, autophagy, and metabolism in primary brown adipocytes and BAT and found that T3 increased fatty acid oxidation and mitochondrial respiration as well as autophagic flux, mitophagy, and mitochondrial biogenesis. Interestingly, there was no significant induct...
Source: Autophagy - September 13, 2018 Category: Cytology Authors: Yau WW, Singh BK, Lesmana R, Zhou J, Sinha RA, Wong KA, Wu Y, Bay BH, Sugii S, Sun L, Yen PM Tags: Autophagy Source Type: research

Oxidative stress associated with aging activates Protein Kinase C Epsilon, leading to cilia slowing.
Abstract Older people are four times more likely to develop pneumonia than younger people. As we age, many components of pulmonary innate immunity are impaired, including slowing of mucociliary clearance. Ciliary beat frequency (CBF) is a major determinant of mucociliary clearance, and it slows as we age. We hypothesized that CBF is slowed in aging due to increased oxidative stress, which activates PKCε signaling. We pharmacologically inhibited PKCε in ex vivo mouse models of aging. We measured a slowing of CBF with aging that was reversed with inhibition using the novel PKC inhibitor, Ro-31-8220, as well as the...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - September 13, 2018 Category: Cytology Authors: Bailey KL, Kharbanda KK, Katafiasz DM, Sisson JH, Wyatt TA Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

MiR-101 inhibits cell proliferation and invasion of pancreatic cancer through targeting STMN1.
In conclusion, miR-101 inhibited PC cell proliferation and invasion via regulating STMN1, which provided a potential therapeutic for PC patients. PMID: 30198871 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - September 12, 2018 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research