Query: siRNA

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Total 15306 results found since Jan 2013.

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10
AbstractTo observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) bothin vivo andin vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p ‐ERK1/2, ERK1/2, and apoptosis‐related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significa ntly inhibited EM‐induced ERK1/2 activation and expressio...
Source: Biotechnology and Applied Biochemistry - December 4, 2018 Category: Biochemistry Authors: Shuangdi Li, Yaping Zhu, Tingting Zhang, Yuanyuan Hang, Qiong Chen, Yuli Jin Tags: Original Article Source Type: research

TGF- β1 Induces Human Tenon's Fibroblasts Fibrosis via miR-200b and Its Suppression of PTEN Signaling.
CONCLUSION: TGF-β1-mediated miR-200b induces HTFs fibrosis through suppressing PTEN signaling pathway. PMID: 30512998 [PubMed - as supplied by publisher]
Source: Current Eye Research - December 4, 2018 Category: Opthalmology Authors: Tong J, Chen F, Du W, Zhu J, Xie Z Tags: Curr Eye Res Source Type: research

Effects of JUN and NFE2L2 knockdown on oxidative status and NFE2L2/AP-1 targets expression in HeLa cells in basal conditions and upon sub-lethal hydrogen peroxide treatment.
Abstract Although NFE2L2 transcription factor is considered to make the most significant contribution to the NFE2L2/AP-1-pathway-dependent antioxidants regulation in the human cell, AP-1 has the potential to provide significant backup and even play an equal role in the cell. Considering this, the present study is focused on revealing how JUN, an AP-1 component, and NFE2L2 contribute to regulation of four target genes containing AREs with embedded TREs-SQSTM1, FTH1, HMOX1 and CBR3 and to cellular oxidative status in general in basal conditions and under pro-oxidative influence. NFE2L2 and JUN were down-regulated in...
Source: Molecular Biology Reports - December 4, 2018 Category: Molecular Biology Authors: Belanova AA, Chmykhalo VK, Makarenko MS, Lyangasova OV, Belousova MM, Aleksandrova AA, Zolotukhin PV Tags: Mol Biol Rep Source Type: research

EDG5 plays an important role in induction and maintenance of pluripotency
Graphical abstract summarizing the main findings generated from this study. AbstractDirect reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high ‐throughput siRNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein–coupled receptors (GPCRs) were identified suggesting an important role for this key signalling pathway during somatic cell induced reprogra...
Source: Stem Cells - December 4, 2018 Category: Stem Cells Authors: Irina Neganova, Lewis Cotts, Peter Banks, Katja Gassner, Lyle Armstrong, Graham Ladds, Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10
AbstractTo observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) bothin vivo andin vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p ‐ERK1/2, ERK1/2, and apoptosis‐related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significa ntly inhibited EM‐induced ERK1/2 activation and expressio...
Source: Biotechnology and Applied Biochemistry - December 4, 2018 Category: Biochemistry Authors: Shuangdi Li, Yaping Zhu, Tingting Zhang, Yuanyuan Hang, Qiong Chen, Yuli Jin Tags: Original Article Source Type: research

EDG5 plays an important role in induction and maintenance of pluripotency
Graphical abstract summarizing the main findings generated from this study. AbstractDirect reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high ‐throughput siRNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein–coupled receptors (GPCRs) were identified suggesting an important role for this key signalling pathway during somatic cell induced reprogra...
Source: Stem Cells - December 4, 2018 Category: Stem Cells Authors: Irina Neganova, Lewis Cotts, Peter Banks, Katja Gassner, Lyle Armstrong, Graham Ladds, Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Involvement of the STAT5-CyclinD/CDK4-pRb pathway in beta cell proliferation stimulated by prolactin during pregnancy.
Abstract During pregnancy, maternal pancreatic beta cells undergo a compensatory expansion in response to the state of insulin resistance, where prolactin (PRL) plays a major role. Retinoblastoma protein (Rb) has been shown to critically regulate islet proliferation and function. The aim of the study was to explore the role of Rb in beta cell mass expansion during pregnancy. During pregnancy, expression of Rb, phospho-Rb (p-Rb), p107 and E2F1 increased, while p130 decreased in maternal islets. With PRL stimulation, induction of Rb expression occurred mainly in the nucleus while p-Rb was predominantly in the cytopl...
Source: Am J Physiol Endocri... - December 4, 2018 Category: Endocrinology Authors: Zhao X, Xu Y, Wu Y, Zhang H, Shi H, Zhu H, Woo M, Wu X Tags: Am J Physiol Endocrinol Metab Source Type: research

B7H6 is a functional ligand for NKp30 in rat and cattle and determines NKp30 reactivity towards human cancer cell lines.
This article is protected by copyright. All rights reserved. PMID: 30512185 [PubMed - as supplied by publisher]
Source: European Journal of Immunology - December 4, 2018 Category: Allergy & Immunology Authors: Bjørnsen EG, Thiruchelvam-Kyle L, Hoelsbrekken SE, Henden C, Saether PC, Boysen P, Daws MR, Dissen E Tags: Eur J Immunol Source Type: research

Self-targeted knockdown of CD44 improves cisplatin sensitivity of chemoresistant non-small cell lung cancer cells
ConclusionsThese results suggest that self-targeted downregulation of chemoresistance-associated cell surface proteins during chemotherapy is an effective therapeutic strategy for overcoming the chemoresistance of NSCLC cells.
Source: Cancer Chemotherapy and Pharmacology - December 4, 2018 Category: Cancer & Oncology Source Type: research

GSE115385 Transcriptomal analysis of the impact of ASAH1 inhibition on skin metastatic melanoma cells
Contributors : J Leclerc ; N Nottet ; C Bertolotto ; R BallottiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo assess the impact of ASAH1 inhibition skin metastatic melanoma, we transfected three different human skin metastatic melanoma cell lines (501Mel, WM3912 and WM8) with a siRNA designed against ASAH1 (50nM) or a siRNA control (50nM) for 48h.
Source: GEO: Gene Expression Omnibus - December 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts
This study shows that chemical hypoxia originating from HIF‐1α upregulation within the periprosthetic tissue is related to cobalt wear debris and highlights TRPM7 as an important key mediator in this context. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 00B: 0 00–000, 2018.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - December 3, 2018 Category: Materials Science Authors: Constantin R ömmelt, Thomas Munsch, Andreas Drynda, Volkmar Lessmann, Christoph H. Lohmann, Jessica Bertrand Tags: Original Research Reports Source Type: research

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.
Abstract The recruitment of tissue-resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, co-ordinated by growth factors and the extracellular matrix (ECM). Integrin-mediated cell adhesion to the ECM provides essential signals for migration. We have been suggested that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary...
Source: J Cell Mol Med - December 3, 2018 Category: Molecular Biology Authors: Kawamura M, Yamamoto T, Yamashiro K, Kochi S, Yoshihara-Hirata C, Ideguchi H, Aoyagi H, Omori K, Takashiba S Tags: J Cell Mol Med Source Type: research

Effect of atorvastatin on cardiomyocyte hypertrophy through suppressing MURC induced by volume overload and cyclic stretch.
Abstract MURC (muscle-restricted coiled-coil protein) is a hypertrophy-related gene. Hypertrophy can be induced by mechanical stress. The purpose of this research was to investigate the hypothesis that MURC mediates hypertrophy in cardiomyocytes under mechanical stress. We used the in vivo model of an aortocaval shunt (AV shunt) in adult Wistar rats to induce myocardial hypertrophy. We also used the in vitro model of cyclic stretch in rat neonatal cardiomyocytes to clarify MURC expression and the molecular regulation mechanism. The flexible membrane culture plate seeding with cardiomyocytes Cardiomyocytes seeded o...
Source: J Cell Mol Med - December 3, 2018 Category: Molecular Biology Authors: Cheng WP, Lo HM, Wang BW, Chua SK, Shyu KG Tags: J Cell Mol Med Source Type: research

Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts
This study shows that chemical hypoxia originating from HIF‐1α upregulation within the periprosthetic tissue is related to cobalt wear debris and highlights TRPM7 as an important key mediator in this context. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 00B: 0 00–000, 2018.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - December 3, 2018 Category: Materials Science Authors: Constantin R ömmelt, Thomas Munsch, Andreas Drynda, Volkmar Lessmann, Christoph H. Lohmann, Jessica Bertrand Tags: Original Research Reports Source Type: research

The AIB1siRNA-loaded hyaluronic acid-assembled PEI/heparin/Ca2+ nanocomplex as a novel therapeutic strategy in lung cancer treatment.
Abstract In the present study, AIB1siRNA‑loaded polyethyleneimine (PEI)/heparin/Ca2+ nanoparticles (NPs) were successfully prepared and evaluated for their efficacy in lung cancer cells. The results demonstrated that the PEI and heparin complex reduced the toxic effect in cancer cells while maintaining its transfection efficiency. A nanosized particle of ~25 nm was formulated and siRNA was demonstrated to possess excellent binding efficiency in the particles. Confocal microscopy revealed that fluorescein‑labeled (FAM)‑small interfering (si)RNA dissociated from the HA‑PEI/heparin/Ca2+/siRNA (CPH‑siH) NPs...
Source: International Journal of Molecular Medicine - December 3, 2018 Category: Molecular Biology Authors: Hao Y, Gao Y, Wu Y, An C Tags: Int J Mol Med Source Type: research

Downregulation of RTN1-C attenuates MPP+-induced neuronal injury through inhibition of mGluR5 pathway in SN4741 cells.
In conclusion, our data suggest that downregulation of RTN1-C protects SN4741 cells against MPP+ through mGluR5-mediated preservation of Ca2+ homeostasis. Therefore, RTN1-C might represent a therapeutic target for the treatment of neuronal injury in experimental PD models. PMID: 30521940 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - December 3, 2018 Category: Neurology Authors: Chang J, Zhang XL, Yu H, Chen J Tags: Brain Res Bull Source Type: research

Carboxypeptidase E- ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.
In this study, we investigated the effect of CPE-ΔN on cell migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells, and illustrated the molecular mechanisms. We first constructed CPE-ΔN overexpressing human OS cell lines (143B and U2OS cells), and found that ectopic CPE-ΔN expression in OS cells enhanced cellular migratory and invasiveness abilities, and promoted EMT process. Further, overexpression of CPE-ΔN increased c-myc and nuclear β-catenin levels in OS cells, which suggested CPE-ΔN promoted the activation of Wnt/β-catenin pathway in OS cells. Treatment with β-catenin small interfering RN...
Source: Biochemistry and Cell Biology - December 3, 2018 Category: Biochemistry Authors: Fan S, Gao X, Chen P, Li X Tags: Biochem Cell Biol Source Type: research

Cellular prion protein regulates the differentiation and function of adipocytes through autophagy flux
In this study, we investigated whether normal cellular prion protein (PrP<C>) is involved in the modulation of autophagy and affects adipogenic differentiation in vivo and in vitro. Surprisingly, autophagy flux signals were activated in the adipose tissue of prion protein-deficient mice and PrP<C>-deleted 3T3-L1 adipocytes. The activation of autophagy flux mediated by PrP<C> deletion was confirmed in the adipose tissue via transmission electron microscopy. Adipocyte differentiation factors were highly induced in prion protein-deficient adipose tissue and 3T3-L1 adipocytes. In addition, deletion of prion protein...
Source: Molecular and Cellular Endocrinology - December 2, 2018 Category: Endocrinology Source Type: research

Leukocyte ‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC.
Leukocyte‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC. Oncol Rep. 2018 Nov 20;: Authors: Jingushi K, Uemura M, Nakano K, Hayashi Y, Wang C, Ishizuya Y, Yamamoto Y, Hayashi T, Kinouchi T, Matsuzaki K, Kato T, Kawashima A, Ujike T, Nagahara A, Fujita K, Ueda K, Tsujikawa K, Nonomura N Abstract Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, responsible for approximately 90‑95% of cases. We previously reported a novel method that enables direct extraction of extracellular vesicles (EVs) from surgically resected viable tissues, yielding what...
Source: Oncology Reports - December 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
Authors: Chen Y, Liu Y, Zhou Y, You H Abstract The occurrence of colorectal cancer (CRC) is associated with a variety of oncogenes and tumor‑suppressor genes. As a tumor‑suppressor gene, the liver kinase B1 gene (LKB1, also known as serine/threonine kinase 11, STK11) is closely related to tumor angiogenesis, invasion and metastasis, but its molecular mechanisms remain unclear. The aim of the present study was to investigate the effects of LKB1 on the invasion and metastasis of CRC, and to explore its molecular mechanisms. By detecting the expression of LKB1 in CRC, we can provide a reference index for diagnosin...
Source: Oncology Reports - December 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibition of lactate dehydrogenase A suppresses inflammatory response in RAW 264.7 macrophages.
Authors: Song YJ, Kim A, Kim GT, Yu HY, Lee ES, Park MJ, Kim YJ, Shim SM, Park TS Abstract Lactate is an important metabolite in cellular metabolism and fluctuates in certain disease conditions including cancer and immune diseases. It was hypothesized that a decrease in lactate would modulate the inflammatory response elicited by lipopolysaccharides (LPS) in macrophages. When RAW 264.7 macrophages were treated with FX11, a specific lactate dehydrogenase (LDHA) inhibitor, the expression of the cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX‑2) was downregulated due to reduced cellular l...
Source: Molecular Medicine Reports - December 1, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Down-regulation of microRNA-216a confers protection against yttrium aluminium garnet laser-induced retinal injury via the GDNF-mediated GDNF/GFR α1/RET signalling pathway.
This study aims to elucidate the role of miR-216a in yttrium aluminium garnet (YAG) laser-induced retinal injury by targeting glial cell line-derived neurotrophic factor (GDNF) via GDNF/GDNF family neurotrophic factor receptor α1 (GFRα1)/rearranged during transfection (RET) signalling pathway. Wistar male rats were first randomly assigned into control and model groups. Immunohistochemistry was performed to detect the GDNF positive expression rate and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) staining for apoptotic index (AI) of retinal tissue. Retinal neurons were divided into normal,...
Source: Journal of Biosciences - December 1, 2018 Category: Biomedical Science Authors: Hu XB, Fu SH, Luo QI, He JZ, Qiu YF, Lai W, Zhong M Tags: J Biosci Source Type: research

MiR-92a inhibits fibroblast-like synoviocyte proliferation and migration in rheumatoid arthritis by targeting AKT2.
This study was aimed to investigate the effects of miR-92a on the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs). Our results showed that miR-92a was significantly down-regulated in RA synovial tissue and RA-FLSs, whereas the protein level of AKT2 is increased. Restoration of miR-92a suppressed the proliferation and migration of RA-FLSs. Down-regulation of miR-92a promotes proliferation and migration of normal human FLSs. Dual luciferase reporter gene assay showed that miR-92a could specifically bind with the 30UTR of AKT2 and significantly repressed the luciferase activity. Down...
Source: Journal of Biosciences - December 1, 2018 Category: Biomedical Science Authors: Yu FY, Xie CQ, Jiang CL, Sun JT, Feng HC, Li C, Huang XW Tags: J Biosci Source Type: research

Cytokines promote lipolysis in 3T3-L1 adipocytes through induction of NADPH oxidase 3 expression and superoxide production Research Articles
NADPH oxidase (NOX) enzymes are one of the major superoxide-generating systems in cells. NOX-generated superoxide has been suggested to promote insulin resistance in the liver. However, the role of NOX enzymes in mediating metabolic dysfunction in other insulin target tissues remains unclear. Here, we show that NOX3 expression is induced in differentiated 3T3-L1 adipocytes upon treatment with proinflammatory cytokines. Superoxide production increased concurrently with NOX3 protein expression in cytokine-treated adipocytes, which was inhibited by the NOX inhibitor diphenyleneiodonium (DPI). Treatment of adipocytes with cyto...
Source: The Journal of Lipid Research - December 1, 2018 Category: Lipidology Authors: Issa, N., Lachance, G., Bellmann, K., Laplante, M., Stadler, K., Marette, A. Tags: Research Articles Source Type: research

Biodistribution and Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles In Vitro and In Vivo
This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI) (SPION-PEI) designed for small interfering RNA (siRNA) delivery bothin vitro andin vivo. SPION-PEI/siRNA complexes were prepared at different weight ratios. Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Rats were divided into three groups: a control group, a normal-saline group and a SPION-PEI/siRNA group. After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2018 Category: Research Source Type: research

Myofibril Assembly in Cultured Mouse Neonatal Cardiomyocytes.
Abstract The formation of myofibrils was analyzed in neonatal mouse cardiomyocytes grown in culture and stained with fluorescent antibodies directed against myofibrillar proteins. The cardiomyocyte cultures also were exposed to siRNA probes to test the role of nonmuscle myosin IIB expression in the formation of myofibrils. In culture, new myofibrils formed in the spreading cell margins surrounding contractile myofibrils previously assembled in utero. Observations indicated that assembly of mature myofibrils occurred in three-stages, as previously reported in cultured mouse skeletal muscle. Premyofibrils, character...
Source: Anatomical Record - December 1, 2018 Category: Anatomy Authors: White J, Wang J, Fan Y, Dube DK, Sanger JW, Sanger JM Tags: Anat Rec (Hoboken) Source Type: research

Resolvin D1, but not resolvin E1, transactivates the epidermal growth factor receptor to increase intracellular calcium and glycoconjugate secretion in rat and human conjunctival goblet cells.
CONCLUSIONS: We conclude that in cultured rat and human conjunctival goblet cells, RvD1 activates the EGFR, increases [Ca2+]i, activates AKT and ERK1/2 to stimulate mucin secretion. RvE1 does not transactivate the EGFR to increase [Ca2+]I and stimulate mucin secretion, but does interact with the receptor to increase ERK 1/2 activity. PMID: 30513286 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - December 1, 2018 Category: Opthalmology Authors: Kaye R, Botten N, Lippestad M, Li D, Hodges RR, Utheim TP, Serhan CN, Dartt DA Tags: Exp Eye Res Source Type: research

HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression.
This study aimed to explore the function of HtrA1 in pancreatic cancer cell growth and its underlying mechanism. We found that the expression of HtrA1 was lower in pancreatic cancer tissue compared to the adjacent normal tissue. Consistently, HtrA1 levels were also decreased in two human pancreatic cancer cell lines, PANC-1 and BXPC-3. Moreover, enforced expression of HtrA1 inhibited cell viability and colony formation of PANC-1 and BXPC-3 cells. Overexpression of HtrA1 promoted apoptosis and suppressed migratory ability of tumor cells. On the contrary, siRNA-mediated knockdown of HtrA1 promoted the growth potential of pan...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/ β-catenin pathway.
In conclusion, we demonstrated that GPNMB contributed to the tumorigenesis of cervical cancer, at least in part, by regulating MMP-2/MMP-9 activity in tumor cells via activation of canonical Wnt/β-catenin signaling. This might be a potential therapeutic target for treating human cervical cancer. PMID: 30484490 [PubMed - in process]
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

miR-130b participates in wear particle-induced inflammation and osteolysis via FOXF2/NF- κB pathway.
CONCLUSION: The role of miR-130b/FOXF2/NF-κB pathway in PIO was firstly revealed, which provided new targets for the treatment of periprosthetic osteolysis. PMID: 30488739 [PubMed - as supplied by publisher]
Source: Immunopharmacology and Immunotoxicology - November 30, 2018 Category: Allergy & Immunology Tags: Immunopharmacol Immunotoxicol Source Type: research

B-Cell Receptor-Associated Protein 31 Regulates the Expression of Valosin-Containing Protein Through Elf2
Conclusion: These results indicate that Bap31 regulates the expression of VCP possibly via Elf2 and support the potential molecular function of Bap31 in CNS diseases.Cell Physiol Biochem 2018;51:1799 –1814
Source: Cellular Physiology and Biochemistry - November 30, 2018 Category: Cytology Source Type: research

Alpha-naphthoflavone induces apoptosis through endoplasmic reticulum stress via c-Src-, ROS-, MAPKs-, and arylhydrocarbon receptor-dependent pathways in HT22 hippocampal neuronal cells.
Abstract α-Naphthoflavone (αNF) is a prototype flavone, also known as a modulator of aryl hydrocarbon receptor (AhR). In the present study, we investigated the molecular mechanisms of αNF-induced cytotoxic effects in HT22 mouse hippocampal neuronal cells. αNF induced apoptotic cell death via activation of caspase-12 and -3 and increased expression of endoplasmic reticulum (ER) stress-associated proteins, including C/EBP homologous protein (CHOP). Inhibition of ER stress by treatment with the ER stress inhibitor, salubrinal, or by CHOP siRNA transfection reduced αNF-induced cell death. αNF activated mitogen-a...
Source: Neurotoxicology - November 30, 2018 Category: Neurology Authors: Yu AR, Ju Jeong Y, Hwang CY, Yoon KS, Choe W, Ha J, Kim SS, Kim Pak Y, Yeo EJ, Kang I Tags: Neurotoxicology Source Type: research

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury
AbstractAstrocytes are involved in neuroprotection, and DJ-1 is an important antioxidant protein that is abundantly expressed in reactive astrocytes. However, the role of DJ-1 in astrocytes ’ neuroprotection in cerebral ischemia/reperfusion injury and its potential mechanism is unclear. Thus, to explore effects and mechanisms of DJ-1 on the neuroprotection of astrocytes, we used primary co-cultures of neurons and astrocytes under oxygen and glucose deprivation/reoxygenation in vitro and transient middle cerebral artery occlusion/reperfusion in vivo to mimic ischemic reperfusion insult. Lentiviral was used to inhibit and ...
Source: Journal of Molecular Medicine - November 30, 2018 Category: Molecular Biology Source Type: research

Baicalin inhibits proliferation and promotes apoptosis of vascular smooth muscle cells by regulating the  MEG3/p53 pathway following treatment with ox‑LDL.
Baicalin inhibits proliferation and promotes apoptosis of vascular smooth muscle cells by regulating the MEG3/p53 pathway following treatment with ox‑LDL. Int J Mol Med. 2018 Nov 30;: Authors: Liu Y, Jia L, Min D, Xu Y, Zhu J, Sun Z Abstract Atherosclerosis (AS) is a systemic disease associated with lipid metabolic disorders and abnormal proliferation of smooth muscle cells. Baicalin is a flavonoid compound isolated from the dry roots of Scutellaria baicalensis Georgi and exerts anti‑proliferative effects in various types of cells. However, the effect of baicalin on AS remains unclear. In the pre...
Source: International Journal of Molecular Medicine - November 30, 2018 Category: Molecular Biology Authors: Liu Y, Jia L, Min D, Xu Y, Zhu J, Sun Z Tags: Int J Mol Med Source Type: research

ADAM17 knockdown increases sensitivity of SW480 cells to cetuximad.
CONCLUSIONS: ADAM17 knockdown obviously inhibits EGFR-AKT signaling pathway and increases the sensitivity of SW480 cells to tocetuximad. PMID: 30514687 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Chen Y, Zheng K, Chen Z, Feng H, Fang W, Huang Z Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

SIRT1 participates in epithelial-mesenchymal transition of EC-9706 and Eca-109 cells in vitro by regulating Snail expression.
CONCLUSIONS: SIRT1 participates in the invasion and metastasis of EC-9706 and Eca- 109 cells probably by inducing EMT via regulating the expression of Snail. PMID: 30514680 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Wu Y, Xin D, Liu C, Wang F Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

Expression of secreted frizzled-related protein 4 in DNA mismatch repair-deficient and mismatch repair-proficient colorectal cancers.
CONCLUSIONS: Up-regulation of SFRP4 in dMMR stage Ⅱ colon cancer promotes apoptosis and inhibits proliferation of the cancer cells, and may improve the prognosis of dMMR colon cancer. PMID: 30514676 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Chen K, Liang H, Peng J, Zheng Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

Effects of sera of rats fed with Huganqingzhi tablets on endoplasmic reticulum stress in a HepG2 cell model of nonalcoholic fatty liver disease.
CONCLUSIONS: HGT serum can effectively prevent FFAs-induced steatosis in HepG2 cells by alleviating ER stress, in which PKC-δ may act as an important target. PMID: 30514673 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Yang M, Chen Z, Xiao C, Tang W, Zhou B Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

Insulin-like growth factor-1 activates AMPK to augment mitochondrial function and correct neuronal metabolism in sensory neurons in type 1 diabetes
ConclusionsIn DRG neuron cultures IGF-1 signals via AMPK to elevate mitochondrial function and drive axonal outgrowth. We propose that this signaling axis mediates IGF-1-dependent protection from distal dying-back of fibers in diabetic neuropathy.
Source: Molecular Metabolism - November 29, 2018 Category: Endocrinology Source Type: research

Salvianolic acids improve liver lipid metabolism in ovariectomized rats via blocking STAT-3/SREBP1 signaling
In conclusion, these findings illustrated that salvianolic acids markedly alleviated the lipid metabolism disorders and protected against the postmenopausal obesity. The underlying mechanism was probably associated with the regulation of STAT-3 signaling.
Source: Chinese Journal of Natural Medicines - November 29, 2018 Category: Complementary Medicine Source Type: research

DNA replication licensing proteins: saints and sinners in cancer
Publication date: Available online 28 November 2018Source: Seminars in Cancer BiologyAuthor(s): Muhammad Jameel Mughal, Ravikiran Mahadevappa, Hang Fai KwokAbstractDNA replication is all-or-none process in the cell, meaning, once the DNA replication begins it proceeds to completion. Hence, to achieve maximum control of DNA replication, eukaryotic cells employ a multi-subunit initiator protein complex known as “pre-replication complex or DNA replication licensing complex (DNA replication LC). This complex involves multiple proteins which are origin-recognition complex family proteins, cell division cycle–6, chromatin li...
Source: Seminars in Cancer Biology - November 29, 2018 Category: Cancer & Oncology Source Type: research

Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells
Conclusion: In response to HMGB1 stimulation, HAECs rapidly undergo ectodomain shedding of RAGE and TLR4, and thereby become insensitive to further HMGB1 stimulation. ADAM17, activated through RAGE-p38 MAPK pathway, is implicated in the ectodomain cleavage of the receptors.Cell Physiol Biochem 2018;51:1632 –1644
Source: Cellular Physiology and Biochemistry - November 29, 2018 Category: Cytology Source Type: research

Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis
Publication date: Available online 29 November 2018Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Olivier Monestier, Aurélie Landemaine, Jérôme Bugeon, Pierre-Yves Rescan, Jean-Charles GabillardAbstractThe understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres.To identify new genes controlling myoblast fusion, w...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 29, 2018 Category: Molecular Biology Source Type: research

Long Noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410.
This study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays, and wound-healing a...
Source: Bioscience Reports - November 29, 2018 Category: Biomedical Science Authors: Sun WL, Kang T, Wang YY, Sun JP, Li C, Liu HJ, Yang Y, Jiao BH Tags: Biosci Rep Source Type: research

Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway.
Abstract In humans, mutation of glycine 93 to alanine of Cu++/Zn++ superoxide dismutase type-1 (SOD1-G93 A) has been associated to some familial cases of Amyotrophic Lateral Sclerosis (ALS). Several evidence proposed the involvement of environmental pollutants that like mercury could accelerate ALS symptoms. SH-SY5Y cells stably transfected with SOD1 and G93 A mutant of SOD1 constructs were exposed to non-toxic concentrations (0.01 µM) of ethylmercury thiosalicylate (thimerosal) for 24 hours. Interestingly, we found that thimerosal, in SOD1-G93 A cells, but not in SOD1 cells, reduced cell survival. Furthe...
Source: Neurotoxicology - November 29, 2018 Category: Neurology Authors: Laudati G, Mascolo L, Guida N, Sirabella R, Pizzorusso V, Bruzzaniti S, Serani A, Di Renzo G, Canzoniero LM, Formisano L Tags: Neurotoxicology Source Type: research

Overcoming Resistance to AC0010, a Third Generation of EGFR Inhibitor, by Targeting c-MET andBCL-2.
Abstract AC0010 is a pyrrolopyrimidine-based irreversible inhibitor of epidermal growth factor receptor (EGFR), structurally distinct from previously reported pyrimidine-based irreversible EGFR inhibitors such as osimertinib and rociletinib. AC0010 selectively inhibits EGFR T790M mutation in both preclinical and clinical studies. However, AC0010 treatment eventually triggers drug resistance with unknown mechanism. To this end, we established two H1975 NSCLC-derived lines resistant to AC0010 after a series of drug exposure and selection in either nude-mice xenograft tumor (H1975-P) or cell culture (H1975-AVR) setti...
Source: Neoplasia - November 29, 2018 Category: Cancer & Oncology Authors: Xu W, Tang W, Li T, Zhang X, Sun Y Tags: Neoplasia Source Type: research

Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression
In conclusion, our study suggests that serum EVs retard H9C2 cell senescence through the miR-34a/PNUTS pathway, providing a potential therapy for cardiac aging.
Source: Journal of Cardiovascular Translational Research - November 29, 2018 Category: Cardiology Source Type: research

Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis.
Abstract The understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres. To identify new genes controlling myoblast fusion, we performed a siRNA screen in c2c12 myoblasts. The genes identified during this screen were then studied in vivo by knockdown in zebrafish using morpholino. We found that N-alpha-acetyltransferase 15 (Naa15) knockdown enhanced c2c12 myoblas...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 29, 2018 Category: Biochemistry Authors: Monestier O, Landemaine A, Bugeon J, Rescan PY, Gabillard JC Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: research

Palmatine attenuated dextran sulfate sodium (DSS)-induced colitis via promoting mitophagy-mediated NLRP3 inflammasome inactivation
Publication date: January 2019Source: Molecular Immunology, Volume 105Author(s): Chu-Tian Mai, Mei-Mei Wu, Chun-Li Wang, Zi-Ren Su, Yuan-Yuan Cheng, Xiao-Jun ZhangAbstractActivation of NLRP3 inflammasomes is crucial in the pathological process of Ulcerative colitis (UC), which could be negatively regulated by PINK1/Parkin-driven mitophagy. Palmatine is a herb derived isoquinoline alkaloid with potent anti-inflammatory and anti-bacteria activities. In present study, we evaluated the effect of palmatine on dextran sulfate sodium (DSS)-induced mice colitis and examined whether its effect is exerted by promoting mitophagy-medi...
Source: Molecular Immunology - November 28, 2018 Category: Allergy & Immunology Source Type: research

microRNA-29a-3p, Up-Regulated in Human Gastric Cells and Tissues with < b > < i > H.Pylori < /i > < /b > Infection, Promotes the Migration of GES-1 Cells via A20-Mediated EMT Pathway
Conclusion: The miR-29a-3p may act as a tumor promotive miRNA by regulating cells migration through directly targeting of A20 gene in human gastric epithelial cells infected withH.pylori.Cell Physiol Biochem 2018;51:1250 –1263
Source: Cellular Physiology and Biochemistry - November 28, 2018 Category: Cytology Source Type: research