Query: siRNA

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Resistin up-regulates LPL expression through the PPAR γ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264.7 macrophages.
Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264.7 macrophages. Cytokine. 2019 Mar 26;119:168-174 Authors: Li B, Fang J, He T, Yin S, Yang M, Cui H, Ma X, Deng J, Ren Z, Hu Y, Ye G, Zhang M, Geng Y, Gou L, Zuo Z Abstract Resistin is a cysteine-rich cytokine, which has been indicated as a mediator of insulin resistance and inflammation. Previous studies demonstrated that lipoprotein lipase (LPL) was an important enzyme that could mediate lipid accumulation in macrophages. Additionally, the intracellular molecules phospha...
Source: Cytokine - March 26, 2019 Category: Molecular Biology Authors: Li B, Fang J, He T, Yin S, Yang M, Cui H, Ma X, Deng J, Ren Z, Hu Y, Ye G, Zhang M, Geng Y, Gou L, Zuo Z Tags: Cytokine Source Type: research

PKM2 Involved in Neuronal Apoptosis on Hypoxic-ischemic Encephalopathy in Neonatal Rats.
In this study, we have investigated the role of PKM2 in cortical neurons which suffered hypoxic-ischemic encephalopathy (HIE) in newborn rats. Immunohistochemistry and Western blot analysis revealed the protein expression of PKM2 peaking at 24 h after HIE. Double immunofluorescence labeling showed that PKM2 was mainly located in the neurons of the ipsilateral cerebral cortex, not in astrocytes or microglia. The increased level of active caspase-3 and the decreased level of phosphorylated AKT (p-AKT) were consistent with the PKM2 expression. TUNEL staining assay showed that PKM2 may participate in neuronal apoptosis in the...
Source: Neurochemical Research - March 25, 2019 Category: Neuroscience Authors: Wu Q, Ge W, Chen Y, Kong X, Xian H Tags: Neurochem Res Source Type: research

MicroRNA-92a promotes vascular smooth muscle cell proliferation and migration through the ROCK/MLCK signalling pathway.
In conclusion, the activation of ROCK/STAT3 and/or MLCK/STAT3 may up-regulate miR-92a expression, which subsequently inhibits KLF4 expression and promotes PDGF-BB-mediated proliferation and migration of VSMCs. This new downstream node in the ROCK/MLCK signalling pathway may offer a potential intervention target for treatment of atherosclerosis. PMID: 30907506 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - March 25, 2019 Category: Molecular Biology Authors: Wang J, Zhang C, Li C, Zhao D, Li S, Ma L, Cui Y, Wei X, Zhao Y, Gao Y Tags: J Cell Mol Med Source Type: research

Tumor Chemosensitization through Oncogene Knockdown Mediated by Unique α-Tocopherylated Cationic Geminis.
Abstract Herein, siRNA transfection efficiency of a unique set of α-tocopherylated gemini lipids has been established in vitro and in vivo. High efficacy of oncogene silencing achieved using the biomacromolecular assembly, formed from siRNA complexes of co-liposomes containing an α-tocopherylated gemini lipid, has been utilized for tumor regression via chemosensitization. Delivery studies with the gemini bearing hydroxyethyl headgroup with octamethylene spacer (TH8S) pointed to a higher siRNA transfection efficacy than its analog without hydroxyethyl group (T8S). Owing to p53 upregulation, transfected cells show...
Source: Biomacromolecules - March 25, 2019 Category: Biochemistry Authors: Kamra M, Maiti B, Dixit A, Karande AA, Bhattacharya S Tags: Biomacromolecules Source Type: research

Genes, Vol. 10, Pages 247: Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements
d Zubiaga The Golgi organelle duplicates its protein and lipid content to segregate evenly between two daughter cells after mitosis. However, how Golgi biogenesis is regulated during interphase remains largely unknown. Here we show that messenger RNA (mRNA) expression of GOLPH3 and GOLGA2, two genes encoding Golgi proteins, is induced specifically in G1 phase, suggesting a link between cell cycle regulation and Golgi growth. We have examined the role of E2F transcription factors, critical regulators of G1 to S progression of the cell cycle, in the expression of Golgi proteins during interphase. We show that promoter...
Source: Genes - March 25, 2019 Category: Genetics & Stem Cells Authors: Pe ñalver-González Vallejo-Rodr íguez Mentxaka Fullaondo Iglesias-Ara Field Zubiaga Tags: Article Source Type: research

Staphylococcus aureus Biofilm-Conditioned Medium Impairs Macrophage-Mediated Antibiofilm Immune Response by Upregulating KLF2 Expression Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Staphylococcus aureus infections associated with the formation of biofilms on medical implants or host tissue play a critical role in the persistence of chronic infections. One critical mechanism of biofilm infection that leads to persistent infection lies in the capacity of biofilms to evade the macrophage-mediated innate immune response. It is now increasingly apparent that microorganisms exploit the negative regulatory mechanisms of the pattern recognition receptor (PRR)-mediated inflammatory response to subvert host cell functions by using various virulence factors. However, the detailed molecular mechanism, along with...
Source: Infection and Immunity - March 25, 2019 Category: Infectious Diseases Authors: Alboslemy, T., Yu, B., Rogers, T., Kim, M.-H. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research

Nimotuzumab inhibits epithelial –mesenchymal transition in prostate cancer by targeting the Akt/YB‐1/AR axis
In this study, we presented our novel findings that by targeting the Akt/YB‐1/AR axis, nimotuzumab significantly inhibited EMT of PC cells. Considering that EMT is a critical mechanism contributing to the metastatic spread of cancer cells, nimotuz umab may become a promising therapy for alleviating the malignant progression of PC. © 2019 IUBMB Life, 1–14, 2019
Source: IUBMB Life - March 25, 2019 Category: Research Authors: Sheng Hu, Yi ‐Xing Duan, Qiang Zhou, Yong Wang, Qiang Lu Tags: Research Communication Source Type: research

Inhibition of acid sphingomyelinase activity ameliorates endothelial dysfunction in db/db mice.
Abstract Acid sphingomyelinase (aSMase) plays an important role in endothelial dysfunction. Here, we show that elevated aSMase activity and ceramide content were reduced by desipramine treatment in diabetic animals. The inhibitor of aSMase, desipramine, improved vascular dysfunction in db/db mice. High glucose-induced upregulation of aSMase activity and ceramide levels were restored by treatment with aSMase siRNA or desipramine in endothelial cells. In addition, aSMase siRNA or desipramine treatment increased the release of nitric oxide (NO) and the phosphorylation of endothelial NO synthase (eNOS) in diabetic mou...
Source: Bioscience Reports - March 25, 2019 Category: Biomedical Science Authors: Jiang M, Huang S, Duan W, Liu Q, Lei M Tags: Biosci Rep Source Type: research

Upregulation of microRNA-148a inhibits proliferation, invasion and migration while promoting apoptosis of cervical cancer cells by downregulating RRS1.
CONCLUSION: Our study suggests that miR-148a downregulates RRS1 expression, thereby inhibiting the proliferation, migration and invasion while promoting cell apoptosis of cervical cancer cells. PMID: 30910849 [PubMed - as supplied by publisher]
Source: Bioscience Reports - March 25, 2019 Category: Biomedical Science Authors: Zhang Y, Sun B, Zhao L, Liu Z, Xu Z, Tian Y, Hao C Tags: Biosci Rep Source Type: research

Protective effect of berberine on high glucose and hypoxia-induced apoptosis via the modulation of HIF-1 α in renal tubular epithelial cells.
Protective effect of berberine on high glucose and hypoxia-induced apoptosis via the modulation of HIF-1α in renal tubular epithelial cells. Am J Transl Res. 2019;11(2):669-682 Authors: Zhang X, Guan T, Yang B, Chi Z, Wan Q, Gu HF Abstract Berberine (BBR), derived from Huanglian (Coptis chinensis), is a traditional Chinese herbal medicine. In the current study, we investigated the effects of BBR in high glucose (HG) and hypoxia-induced apoptosis with normal rat renal tubular epithelial (NRK-52E) and human kidney proximal tubular cells (HK-2) and further explored the underlying molecular mechanism of h...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Knockdown of GRHL2 inhibited proliferation and induced apoptosis of colorectal cancer by suppressing the PI3K/Akt pathway.
In conclusion, GRHL2 acted as an oncoprotein through regulating cell proliferation and apoptosis in CRC cells. The PI3K/Akt pathway was closely involved in the effects of GRHL2. Therefore, GRHL2 might be a therapeutic target for the CRC treatment. PMID: 30917932 [PubMed - as supplied by publisher]
Source: Gene - March 24, 2019 Category: Genetics & Stem Cells Authors: Hu F, He Z, Sun C, Rong D Tags: Gene Source Type: research

Prolyl isomerase Pin1 binds to and stabilizes acetyl CoA carboxylase 1 protein, thereby supporting cancer cell proliferation.
Authors: Ueda K, Nakatsu Y, Yamamotoya T, Ono H, Inoue Y, Inoue MK, Mizuno Y, Matsunaga Y, Kushiyama A, Sakoda H, Fujishiro M, Takahashi SI, Matsubara A, Asano T Abstract The prolyl isomerase Pin1 expression level is reportedly increased in most malignant tissues and correlates with poor outcomes. On the other hand, acetyl CoA carboxylase 1 (ACC1), the rate limiting enzyme of lipogenesis is also abundantly expressed in cancer cells, to satisfy the demand for the fatty acids (FAs) needed for rapid cell proliferation. We found Pin1 expression levels to correlate positively with ACC1 levels in human prostate cancers, ...
Source: Oncotarget - March 23, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Knockdown of serpin peptidase inhibitor clade C member  1 inhibits the growth of nasopharyngeal carcinoma cells.
Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep. 2019 Mar 14;: Authors: Xu J, Ying Y, Xiong G, Lai L, Wang Q, Yang Y Abstract Nasopharyngeal carcinoma (NPC) is a type of cancer originating in the nasopharynx. There are no NPC‑specific treatments available at present. Serpin peptidase inhibitor clade C member 1 (SERPINC1) serves roles in anticoagulation and anti‑inflammation. The aim of the present study was to investigate the role of SERPINC1 in the proliferation and apoptosis of NPC cells. Tumor and adjacent healthy ti...
Source: Molecular Medicine Reports - March 23, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Accumulation of hyaluronic acid in stromal cells modulates osteoclast formation by regulation of receptor activator of nuclear factor kappa-B ligand expression.
In this study, we investigated the effect and underlying mechanisms of HA accumulation on the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) induced by 1α,25(OH)2D3 and dexamethasone in stromal cells, which support osteoclastogenesis. Degradation of HA by hyaluronidase (HA'ase) treatment enhanced the expression of RANKL in ST2 cells stimulated with 1α,25(OH)2D3 and dexamethasone. Down-regulation of hyaluronan synthase 2 (HAS2) expression by siRNA also stimulated RANKL expression induced by 1α,25(OH)2D3 and dexamethasone. Results from a cell co-culture system with bone marrow cell showed that 1...
Source: Biochemical and Biophysical Research communications - March 23, 2019 Category: Biochemistry Authors: Nakao Y, Hikiji H, Okinaga T, Takeuchi J, Habu M, Yoshiga D, Yoshioka I, Nishihara T, Ariyoshi W Tags: Biochem Biophys Res Commun Source Type: research

Involvement of mitogen-activated protein kinase pathways in ferrous iron-induced aquaporin-4 expression in cultured astrocytes.
In this study, we investigated the effect of ferrous iron overload on AQP4 expression in cultured mouse astrocytes. Primary cultures of astrocytes were exposed to ferrous iron, and the expression of AQP4 as well as the swelling of astrocyte were determined. AQP4 expression was inhibited by small interfering RNA (siRNA). The role of oxidative stress and mitogen-activated protein kinases (MAPKs) signaling pathway in ferrous iron-induced AQP4 expression upregulation were further studied. Ferrous iron exposure induced astrocyte death as well as cell swelling, and increased AQP4 expression. AQP4 gene silencing after siRNA trans...
Source: Neurotoxicology - March 23, 2019 Category: Neurology Authors: Zhong Z, Sun Y, Wang B, Sun Q, Yang G, Bian L Tags: Neurotoxicology Source Type: research

Age-dependent expression of the Vitamin D receptor and the protective effect of Vitamin D receptor activation on H2O2-induced apoptosis in rat intervertebral disc cells
Publication date: Available online 21 March 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Tong Tong, Zhihui Liu, Hua Zhang, Jinglei Sun, Dongxia Zhang, Feng Wang, Dechao Miao, Yong ShenAbstractAccumulating evidence shows that genetic polymorphism of the vitamin D receptor (VDR) gene is associated with intervertebral disc degeneration (IDD), implying that VDR may be involved in the pathogenesis of IDD. However, the exact relationship between VDR and IDD remains unknown. The aim of this study was to investigate the age-dependent expression of VDR in rat intervertebral discs and to determine ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - March 22, 2019 Category: Biochemistry Source Type: research

O-GlcNAcylation disrupts STRA6-retinol signals in kidneys of diabetes
ConclusionsO-GlcNAcylation significantly modified STRA6 and RALDH1, suppressed RBP4 binding activity, and disrupted retinol signals in the kidney of diabetes.General significanceThis study first indicates that STRA6-retinol signals were directly disrupted by O-GlcNAcylation in diabetic kidney.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 22, 2019 Category: Biochemistry Source Type: research

Cardiac myocyte-specific expression of beta3-adrenergic receptors sustains AMPK activation and glucose uptake while reducing hypertrophy following pressure overload
ConclusionWe conclude that cardiac myocyte-specific expression of B3AR concurrently prevents the development of insulin resistance and maintains AMPK activation contributing to the protection from hypertrophic remodeling under hemodynamic stress.
Source: Archives of Cardiovascular Diseases Supplements - March 22, 2019 Category: Cardiology Source Type: research

Modulation of GSK - 3β/β - catenin cascade by commensal bifidobateria plays an important role for the inhibition of metaflammation-related biomarkers in response to LPS or non-physiological concentrations of fructose: An in vitro study
ConclusionWe describe a regulatory mechanism in which commensal bifidobacteria prevent LPS- and/or fructose-mediated transcriptional activation of metabolic disorder-causing factors (IL-1β, IL-6, TNF-α) and disruption of normal cellular energy metabolism by attenuating NF-kB activity and interfering with the Wnt/β-catenin pathway. The latter positively regulates NF-κB activity, thereby simultaneously reducing cellular triglyceride concentrations and enhancing cellular glucose consumption. This may be a molecular basis by which commensal bifidobacteria enhance intrinsic cellular tolerance against excess consumption of e...
Source: PharmaNutrition - March 22, 2019 Category: Nutrition Source Type: research

MST2 kinase regulates osteoblast differentiation by phosphorylating and inhibiting Runx2 in C2C12  cells.
The objective of this study was to investigate whether the MST pathway could modulate Runx2 and osteoblast differentiation. First, we found that Runx2 interacted with MST2 and SAV1 via the WW domain of SAV1 and amino acid 292-445 of Runx2 containing a PY motif. Results of OSE luciferase reporter assay revealed that co-expression of MST2 and SAV1 inhibited the transcriptional activity of Runx2 whereas siRNA-mediated down-regulation of Mst1 and Mst2 increased its activity. MST2 and SAV1 significantly reduced mRNA levels of osteoblast differentiation marker genes such as alkaline phosphatase and osteocalcin in differentiating...
Source: Biochemical and Biophysical Research communications - March 22, 2019 Category: Biochemistry Authors: Won GW, Sung M, Lee Y, Lee YH Tags: Biochem Biophys Res Commun Source Type: research

Slow relaxation during and after perturbation of bistable kinetics of gene expression.
Abstract After a short perturbation of a bistable genetic network, it returns to its initial steady state or transits to another steady state. The time scale characterizing such transient regimes can be appreciably longer compared to those of the degradation of the perturbed mRNAs and proteins. The author shows in detail the specifics of this slowdown of the transient kinetics using mean-field kinetic equations and Monte Carlo simulations. Attention is focused on nanocarrier-mediated delivery and release of short non-coding RNA (e.g., miRNA or siRNA) into cells with subsequent suppression of the populations of the...
Source: European Biophysics Journal : EBJ - March 22, 2019 Category: Physics Authors: Zhdanov VP Tags: Eur Biophys J Source Type: research

Ochratoxin A causes mitochondrial dysfunction, apoptotic and autophagic cell death and also induces mitochondrial biogenesis in human gastric epithelium cells.
Abstract Ochratoxin A (OTA) is a common natural contaminant found in human and animal food worldwide. Our previous work has shown that OTA can cause oxidative DNA damage, G2 arrest and malignant transformation of human gastric epithelium (GES-1) cells. Mitochondria are considered to be target for the action of many cytotoxic agents. However, the role of mitochondria in the cytotoxicity of OTA remains unknown. The aim of this study is to explore the putative role of mitochondria on OTA cytotoxicity by analyzing mitochondrial changes in GES-1 cells. The results showed that OTA treatment (5, 10, 20 µM) for differen...
Source: Archives of Toxicology - March 22, 2019 Category: Toxicology Authors: Li Q, Dong Z, Lian W, Cui J, Wang J, Shen H, Liu W, Yang J, Zhang X, Cui H Tags: Arch Toxicol Source Type: research

MK5 haplodeficiency decreases collagen deposition and scar size during post-myocardial infarction wound repair.
Abstract MK5 is a protein serine/threonine kinase activated by p38, ERK3, and ERK4 MAPKs. MK5 mRNA and immunoreactivity are detected in mouse cardiac fibroblasts and MK5-haplodeficiency attenuates the increase in collagen 1-a1 mRNA evoked by pressure overload. The present study examined the effect of MK5 haplodeficiency on reparative fibrosis following myocardial infarction (MI). Twelve-week-old MK5+/- and wild-type littermate (MK5+/+) mice underwent ligation of the left anterior descending coronary artery (LADL). Surviving mice were sacrificed 8- or 21-days post-MI. Survival rates did not differ significantly bet...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 22, 2019 Category: Physiology Authors: Nawaito S, Sahadevan P, Clavet-Lanthier MÉ, Pouliot P, Sahmi F, Shi Y, Gillis MA, Lesage F, Gaestel M, Sirois MG, Calderone A, Tardif JC, Allen BG Tags: Am J Physiol Heart Circ Physiol Source Type: research

Cancers, Vol. 11, Pages 406: Alpha6-Integrin Regulates FGFR1 Expression through the ZEB1/YAP1 Transcription Complex in Glioblastoma Stem Cells Resulting in Enhanced Proliferation and Stemness
Catherine SEVA Glioblastoma (GBM) is the most lethal primary brain tumor in adults and is known to be particularly aggressive and resistant to anti-cancer therapies, mainly due to the presence of GBM stem cells (GBMSC). By in vitro approaches supported by analysis from patients’ databases, we determined how α6-integrin and Fibroblast Growth Factor Receptor 1 (FGFR1) work in concert to regulate proliferation and stemness of GBMSC. We showed that α6-integrin regulates the expression of FGFR1 and its target gene Fokhead Box M1 (FOXM1) via the ZEB1/YAP1 transcription complex. Thes...
Source: Cancers - March 22, 2019 Category: Cancer & Oncology Authors: Aline KOWALSKI-CHAUVEL Valerie GOUAZE-ANDERSSON Laurent BARICAULT Elodie MARTIN Caroline DELMAS Christine TOULAS Elizabeth COHEN-JONATHAN-MOYAL Catherine SEVA Tags: Article Source Type: research

The role of autophagy in the overexpression of MUC5AC in patients with chronic rhinosinusitis.
CONCLUSIONS: In HNE-induced CRS, autophagy increases the secretion of MUC5AC by promoting the phosphorylation of JNK and c-Jun. PMID: 30909132 [PubMed - as supplied by publisher]
Source: International Immunopharmacology - March 22, 2019 Category: Allergy & Immunology Authors: Ye Y, Zhao J, Ye J, Jiang X, Liu H, Xie Y, Zhang J, Luo Q Tags: Int Immunopharmacol Source Type: research

Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and  mechanism in gastric cancer cell growth and metastasis.
CONCLUSION: Collectively, our study highlights that the high expression of miR-27a indicates the poor efficacy and prognosis of neoadjuvant chemotherapy in GC patients. Down-regulation of miR-27a can inhibit the growth and metastasis of GC cells via up-regulation of SFRP1. PMID: 30902884 [PubMed - as supplied by publisher]
Source: Bioscience Reports - March 22, 2019 Category: Biomedical Science Authors: Xu C, Cheng H, Li N, Zhou N, Tang X Tags: Biosci Rep Source Type: research

LincRNA-p21 suppresses glutamine catabolism and bladder cancer cell growth through inhibiting glutaminase (GLS) expression.
In this study, we investigated the involvement of lincRNA-p21 and glutamine catabolism in BC cell growth and found that ectopic linRNA-p21 expression reduced the proliferation and growth of BIU87 and 5637 cells. Opposite results were observed in lincRNA-p21 silenced J82 and T24 cells. The expression of glutaminase (GLS), intracellular level of glutamate and α-KG were negatively regulated by lincRNA-p21. GLS over-expression reversed the suppressive function of lincRNA-p21 on BC cell growth and proliferation. In contrast, GLS reduction by siRNA blunted the viability of lincRNA-p21 lowly-expressed BC cells. Furthermore, linc...
Source: Bioscience Reports - March 22, 2019 Category: Biomedical Science Authors: Zhou Q, Zhan H, Lin F, Liu Y, Yang K, Gao Q, Ding M, Liu Y, Huang W, Cai Z Tags: Biosci Rep Source Type: research

FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Tianming Deng, Yanhong Wang, Chongchong Wang, Hua YanAbstractEndoplasmic reticulum (ER) stress and subsequent apoptosis play a vital role in myocardial ischemia reperfusion (IR) injury. Fatty acid binding protein 4 (FABP4) may induce ER stress. The aim of this study was to investigate the mechanism and effect of FABP4 on IR injury in vitro. Rat H9c2 cells were exposed to hypoxia reoxygenation (HR) to create an IR model in vitro. FABP4 was overexpressed in HR-injured H9c2 cells. Transfection with FABP4 siRNA increased cell viability and decrease...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research

Z-Guggulsterone attenuates glucocorticoid-induced osteoporosis through activation of Nrf2/HO-1 signaling
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Yier Xu, Jian Guan, Jianyu Xu, Shuilin Chen, Guicai SunAbstractAimsThe present study aims to investigate the protective effect and underlying mechanism of Z-Guggulsterone (Z-GS), an active component from myrrh, on glucocorticoid-induced osteoporosis (GIO).Main methodsGIO rats were used to simulate osteoporosis in vivo while MC3T3-E1 cells were induced to osteoblast differentiation and treated with dexamethasone to simulate osteoporosis in vitro. The rats and cells were treated with Z-GS according to the protocol. The bone mineral density, biome...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research

Co-Delivery of Gemcitabine and Mcl-1 SiRNA via Cationic Liposome-Based System Enhances the Efficacy of Chemotherapy in Pancreatic Cancer
Source: Journal of Biomedical Nanotechnology - March 21, 2019 Category: Nanotechnology Authors: Wang, Yanbing Gao, Fenghua Jiang, Xingwei Zhao, Xiao Wang, Yu Kuai, Qiyuan Nie, Guangjun He, Min Pan, Yingjie Shi, Wei Ren, Suping Yu, Qun Tags: Articles Source Type: research

Endoplasmic reticulum-associated degradation potentiates the infectivity of influenza A virus by regulating the host redox state.
Abstract During influenza A virus (IAV) infection, significant effects of oxidative stress often emerge due to the disruption of the redox balance. Reactive oxygen species (ROS) generated during IAV infection have been known to exert various effects on both the virus and host tissue. However, the mechanisms underlying the accumulation of ROS and their physiological significance in IAV infection have been extensively studied but remain to be fully understood. Here, we show that the levels of Sp1, a key controller of Cu-Zn superoxide dismutase (SOD1) gene expression, and SOD1 are mainly dependent upon the activity o...
Source: Free Radical Biology and Medicine - March 21, 2019 Category: Biology Authors: Jung KI, Shin N, Ko DH, Pyo CW, Choi SY Tags: Free Radic Biol Med Source Type: research

Tuftelin Is Required for NGF-Induced Differentiation of PC12 Cells
AbstractNerve growth factor (NGF) promotes pleiotropic gene transcription-dependent biological effects, in neuronal and non-neuronal cells, including survival, proliferation, differentiation, neuroprotection, pain, and angiogenesis. It is hypothesized that during odontogenesis, NGF may be implicated in morphogenetic and mineralization events by affecting proliferation and/or differentiation of dental cells. Tuftelin belongs to the enamel associated teeth proteins and is thought to play a role in enamel mineralization. We previously reported that tuftelin transcript and protein, which are ubiquitously expressed in various t...
Source: Journal of Molecular Neuroscience - March 21, 2019 Category: Neuroscience Source Type: research

OTOR in breast carcinoma as a potent prognostic predictor correlates with cell proliferation, migration and invasion.
This study aimed to investigate clinicopathologic significance of OTOR in breast infiltrating ductal carcinoma (IDC) with high metastasis and unveil its biological function in BC. OTOR was highly overexpressed in BC tissues and cells compared with normal samples. OTOR overexpression was associated with a few of clinicopathologic characteristics and worse overall survival (OS) of IDC breast patients. Silencing of OTOR using siRNA tool impeded BC cells proliferation, migration and invasion by CCK-8, colony formation, wound-healing and transwell assays, which might be regulated by mitogen-activated protein kinase/extracellula...
Source: Biochemistry and Cell Biology - March 21, 2019 Category: Biochemistry Authors: Wang YF, Han J Tags: Biochem Cell Biol Source Type: research

Cationic lipoplexes for treatment of cancer stem cell-derived murine lung tumors
This study outlines the fabrication of a lipoplex of SOX2 small interfering RNA (CL-siSOX2) for targeted treatment of SOX2-enriched, CSC-derived orthotopic and xenograft lung tumors in CB-17 SCID mice. CL-siSOX2 induced tumor contraction in cisplatin-naïve and cisplatin-treated groups by 85% and 94% respectively. Reduction in tumor weight and volume following treatment with CL-siSOX2 was associated with reduced protein expression of SOX2 and markers of tumor initiation, inflammation, invasion and metastasis in mice tumor xenografts. In addition, histological staining of lung tumor sections showed reduction in SOX2 express...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 20, 2019 Category: Nanotechnology Source Type: research

Activation of Nrf2 signaling by Icariin protects against 6 ‐OHDA‐induced neurotoxicity
This study aimed at analyzing whether ICA evoked neuroprotection against 6‐hydroxydopamine (6‐OHDA)‐induced neu rotoxicity in PC12 cells and the mechanisms of action. ICA protected against 6‐OHDA‐induced neuronal damage, accompanied by the inhibition of cell apoptosis through the marked decreases in the Bax/Bcl‐2 ratio, cytochrome C release and caspase‐3 cleavage. In addition, the activation of Nrf2 s ignaling pathway was responsible for ICA‐mediated neuroprotection. First, ICA relieved reactive oxygen species (ROS) accumulation and increased superoxide dismutase (SOD) generation via the activation of Nrf2 ...
Source: Biotechnology and Applied Biochemistry - March 20, 2019 Category: Biochemistry Authors: Li Zhu, DaiDi Li, Ce Chen, GuoQing Wang, JingShan Shi, Feng Zhang Tags: Original Article Source Type: research

Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.
Abstract The apically localized riboflavin (RF) transporter-3 (RFVT-3) is involved in intestinal absorption of vitamin B2. Previous studies have characterized different physiological/biological aspects of the RFVT-3, but there is a lack of knowledge regarding possible existence of interacting partner(s) and consequence of interaction (s) on its function/cell biology. To address the latter, we performed yeast two-hybrid (Y2H) screening of a human colonic cDNA library and have identified transmembrane protein 237 (TMEM237) as a putative interactor with the human (h)RFVT-3; the interaction was further confirmed via "...
Source: Am J Physiol Cell Ph... - March 20, 2019 Category: Cytology Authors: Sabui S, Subramanian VS, Pham Q, Said HM Tags: Am J Physiol Cell Physiol Source Type: research

Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1.
Abstract Defective tight junction (TJ) barrier is a key pathogenic factor for Inflammatory Bowel Disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy related protein ATG6/beclin 1, a key protein in the autophagy pathway, also plays a role in the endocytic pathway. The constitutive role of beclin 1 in intestinal TJ barrier is not known. In Caco-2 cells, beclin 1 was found to be co-immunoprecipitated with the TJ protein occludin and co-localized with occludin on the membrane. Treatment of Caco-2 cells with beclin 1 peptide (Tat-be...
Source: Am J Physiol Cell Ph... - March 20, 2019 Category: Cytology Authors: Wong M, Ganapathy AS, Suchanec E, Laidler L, Ma T, Nighot PK Tags: Am J Physiol Cell Physiol Source Type: research

Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription
In this study, the potential functions of the M protein in the nucleus were investigated. We first demonstrate that nuclear localization of the M protein could not only promote the cytopathogenicity of NDV but also increase viral RNA synthesis and transcription efficiency in DF-1 cells. Using microarray analysis, we found that nuclear localization of the M protein might inhibit host cell transcription, represented by numerous up-regulating genes associated with transcriptional repressor activity and down-regulating genes associated with transcriptional activator activity. The role of representative up-regulated gene prospe...
Source: Veterinary Research - March 20, 2019 Category: Veterinary Research Source Type: research

Activation of Nrf2 signaling by Icariin protects against 6 ‐OHDA‐induced neurotoxicity
This study aimed at analyzing whether ICA evoked neuroprotection against 6‐hydroxydopamine (6‐OHDA)‐induced neu rotoxicity in PC12 cells and the mechanisms of action. ICA protected against 6‐OHDA‐induced neuronal damage, accompanied by the inhibition of cell apoptosis through the marked decreases in the Bax/Bcl‐2 ratio, cytochrome C release and caspase‐3 cleavage. In addition, the activation of Nrf2 s ignaling pathway was responsible for ICA‐mediated neuroprotection. First, ICA relieved reactive oxygen species (ROS) accumulation and increased superoxide dismutase (SOD) generation via the activation of Nrf2 ...
Source: Biotechnology and Applied Biochemistry - March 20, 2019 Category: Biochemistry Authors: Li Zhu, DaiDi Li, Ce Chen, GuoQing Wang, JingShan Shi, Feng Zhang Tags: Original Article Source Type: research

miR3609 sensitizes breast cancer cells to adriamycin by blocking the programmed death-ligand 1 immune checkpoint.
Abstract Chemoresistance is the leading cause of breast cancer therapy failure, and studies of the mechanisms underlying chemoresistance and the treatment of drug-resistant tumors remain challenging. In the present study, we discovered a novel microRNA, miR3609, that influences the malignancy of breast cancer. Our results showed miR-3609 expression was lower in resistant breast cancer cells than in sensitive breast cancer cells (MCF-7), while PD-L1 expression was higher in resistant breast cancer cells than in sensitive breast cancer cells. Co-transfection of a miR-3609 plasmid with a luciferase construct containi...
Source: Experimental Cell Research - March 20, 2019 Category: Cytology Authors: Li D, Wang X, Yang M, Kan Q, Duan Z Tags: Exp Cell Res Source Type: research

The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/ β-catenin signaling.
In conclusion, the present study demonstrated that Wnt10a may have an oncogenic role during carcinogenesis of CRC through activation of Wnt/β-catenin signaling. PMID: 30881490 [PubMed]
Source: Oncology Letters - March 19, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MicroRNA 4651 regulates nonsense-mediated mRNA decay by targeting SMG9 mRNA.
Abstract Nonsense-mediated mRNA decay (NMD) is originally identified as a conserved RNA surveillance mechanism that rapidly degrades aberrant mRNA containing premature termination codons (PTCs). However, the molecular regulation mechanisms by which microRNAs inhibit NMD has not been well understood. Here we identified that miR-4651 participated in the NMD pathway by downregulating expression levels of SMG9. We provided evidences that (1) Overexpression of miR-4651 mimic significantly inhibited the expression of SMG9 (P < 0.05); (2) NMD substrates genes, TBL2 and GADD45B were both increased at mRNA and prote...
Source: Gene - March 19, 2019 Category: Genetics & Stem Cells Authors: Tan Y, Ma Z, Jin Y, Zong R, Wu J, Ren Z Tags: Gene Source Type: research

Cancers, Vol. 11, Pages 386: Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein
This study reveals that the regulation of radiosensitivity of cancer cells is yet another function of TCTP. The relationship between endogenous TCTP levels and sensitivity to radiation was examined in breast cancer cell lines (T47D, MDA-MB-231, and MCF7) and lung cancer cells lines (A549, H1299, and H460). Cancer cells with high expression levels of TCTP were more resistant to radiation. TCTP overexpression inhibited radiation-induced cell death, while silencing TCTP led to an increase in radiosensitivity. DNA damage in the irradiated TCTP-silenced A549 cells was greater than in irradiated control shRNA-transfected A549 ce...
Source: Cancers - March 19, 2019 Category: Cancer & Oncology Authors: Jiwon Jung Ji-Sun Lee Yun-Sil Lee Kyunglim Lee Tags: Article Source Type: research

TRPM2 mediates distruption of autophagy machinery and correlates with the grade level in prostate cancer
ConclusionWe showed that the autophagy –TRPM2 association may take place in the molecular basis of PCa and accordingly this connection may be targeted as a new therapeutic approach in PCa.
Source: Journal of Cancer Research and Clinical Oncology - March 19, 2019 Category: Cancer & Oncology Source Type: research

FER1L4/miR-372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells.
In this study, it was found that the expression of LncRNA FER1L4 is upregulated in high-grade gliomas than in low-grade cases and that a high expression of LncRNA FER1L4 predicts poor prognosis of gliomas. Meanwhile, in vitro study suggests that expression of FER1L4 with SiRNA knockdown obviously suppresses cell cycle and proliferation. It is further demonstrated by experiments that the FER1L4 knockdown suppresses growth of in vivo glioma. Besides, it is found in our study that LncRNA FER1L4 expression is positively correlated with E2F1 mRNA expression. After knockdown of FER1L4 expression, E2F1 expression is significantly...
Source: J Cell Mol Med - March 19, 2019 Category: Molecular Biology Authors: Xia L, Nie D, Wang G, Sun C, Chen G Tags: J Cell Mol Med Source Type: research

Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/ β‐Catenin in Primary Cilia of Osteoblasts
ABSTRACTExtremely low ‐frequency electromagnetic fields have been considered a potential candidate for the prevention and treatment of osteoporosis; however, their action mechanism and optimal magnetic flux density (intensity) parameter are still elusive. The present study found that 50‐Hz sinusoidal electromagnetic fields (SEMFs) at 1.8 mT increased the peak bone mass of young rats by increasing bone formation. Gene array expression studies with femoral bone samples showed that SEMFs increased the expression levels of collagen‐1α1 and Wnt10b, a critical ligand of the osteogenic Wnt/β‐catenin pathway. Con sistent...
Source: Journal of Bone and Mineral Research - March 19, 2019 Category: Orthopaedics Authors: Jian Zhou, Yu ‐Hai Gao, Bao‐Ying Zhu, Jia‐Le Shao, Hui‐Ping Ma, Cory J Xian, Ke‐Ming Chen Tags: Original Article Source Type: research

GSE118101 Next Generation Sequencing upon siRNA-mediated knockdown of DRAIC in MCF-7 cells.
Contributor : Imke TiessenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe goal of this study is to examine the effect of DRAIC knockdown on the MCF-7 transcriptome.
Source: GEO: Gene Expression Omnibus - March 18, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Viruses, Vol. 11, Pages 271: Mosquito Small RNA Responses to West Nile and Insect-Specific Virus Infections in Aedes and Culex Mosquito Cells
P. Pijlman Small RNA mediated responses are essential for antiviral defence in mosquitoes, however, they appear to differ per virus-vector combination. To further investigate the diversity of small RNA responses against viruses in mosquitoes, we applied a small RNA deep sequencing approach on five mosquito cell lines: Culex tarsalis CT cells, Aedes albopictus U4.4 and C6/36 cells, Ae. aegypti Aag2 cells (cleared from cell fusing agent virus and Culex Y virus (CYV) by repetitive dsRNA transfections) and Ae. pseudoscutellaris AP-61 cells. De novo assembly of small RNAs revealed the presence of Phasi Charoen-like virus (P...
Source: Viruses - March 18, 2019 Category: Virology Authors: Giel P. G öertz Pascal Miesen Gijs J. Overheul Ronald P. van Rij Monique M. van Oers Gorben P. Pijlman Tags: Article Source Type: research

Characterization of In vitro inhibitory effects of consensus short interference RNAs against non-structural 5B gene of hepatitis C virus 1a genotype
Conclusions: Stable Huh-7 cells persistently expressing NS5B protein should be helpful for molecular pathogenesis of HCV infection and development of anti-HCV drug screening assays. The siRNA was effective against NS5B and could be considered as an adjuvant therapy along with other promising anti-HCV regimens.
Source: Indian Journal of Medical Microbiology - March 18, 2019 Category: Microbiology Authors: Imran Shahid Waleed Hassan Almalki Munjed M Ibrahim Sultan Ahmad Alghamdi Mohammed H Mukhtar Shaia Saleh R. Almalki Saad Ahmed Alkahtani Mohammad S Alhaidari Source Type: research