Query: siRNA

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Total 13814 results found since Jan 2013.

HRAS as a potential therapeutic target of salirasib RAS inhibitor in bladder cancer.
Abstract The active form of the small GTPase RAS binds to downstream effectors to promote cell growth and proliferation. RAS signal enhancement contributes to tumorigenesis, invasion, and metastasis in various different cancers. HRAS proto-oncogene GTPase (HRAS), one of the RAS isoforms, was the first human oncogene for which mutations were reported in T24 bladder cancer (BC) cells in 1982, and HRAS mutation or upregulation has been reported in several cancers. According to data from The Cancer Genome Atlas, HRAS expression was significantly upregulated in clinical BC samples compared to healthy samples (P=0.0024...
Source: International Journal of Oncology - June 11, 2018 Category: Cancer & Oncology Authors: Sugita S, Enokida H, Yoshino H, Miyamoto K, Yonemori M, Sakaguchi T, Osako Y, Nakagawa M Tags: Int J Oncol Source Type: research

α2 isoform of Na+,K+-ATPase via Na+,Ca2+ exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.
Abstract Oligodendrocytes in the CNS myelinate neuronal axons, facilitating rapid propagation of action potentials. Myelin basic protein (MBP) is an essential component of myelin and its absence results in severe hypomyelination. In oligodendrocyte lineage cell (OLC) monocultures MBP synthesis starts at DIV4. Ouabain (10 nM), a Na+,K+-ATPase (NKA) blocker, stimulates MBP synthesis. As OLCs express the α2 isoform of NKA (α2-NKA) that has a high affinity for ouabain, we hypothesized that α2-NKA mediates this effect. Knockdown of α2-NKA with small interfering (si)RNA (α2-siRNA) significantly potentiated MBP sy...
Source: Cell Calcium - June 10, 2018 Category: Cytology Authors: Hammann J, Bassetti D, White R, Luhmann HJ, Kirischuk S Tags: Cell Calcium Source Type: research

Silicon dioxide nanoparticles induce COX-2 expression through activation of STAT3 signaling pathway in HaCaT cells.
Abstract Silicon dioxide nanoparticles (SiO2-NPs) are widely used in biomedicines and consumer products, such as sunscreens and cosmetics. However, SiO2-NPs can cause adverse effects on human health, depending on the size and concentration of nanoparticles. The present study was aimed at investigating the molecular mechanism underlying SiO2-NPs-induced inflammation in human keratinocyte (HaCaT) cells. Incubation of HaCaT cells with SiO2-NPs induced the expression of cyclooxygenase-2 (COX-2) mRNA and protein. Treatment of cells with SiO2-NPs also induced the phosphorylation, DNA binding and the reporter gene activi...
Source: Toxicology in Vitro - June 9, 2018 Category: Toxicology Authors: Kundu J, Kim DH, Chae IG, Lee JK, Lee S, Jeong CH, Chun KS Tags: Toxicol In Vitro Source Type: research

Targeted inhibition of Axl receptor tyrosine kinase ameliorates anti-GBM-induced lupus-like nephritis.
Abstract Glomerulonephritis (GN) is a typical lesion in autoantibody and immune complex disorders, including SLE. Because the Gas6/Axl pathway has been implicated in the pathogenesis of many types of GN, targeting this pathway might ameliorate GN. Consequently, we have studied the efficacy and mechanism of R428, a potent selective Axl inhibitor, in the prevention of experimental anti-GBM nephritis. Axl upregulation was investigated with Sp1/3 siRNA in the SV40-transformed mesangial cells. For Axl inhibition, a daily dose of R428 (125 mg/kg) or vehicle was administered orally. GN was induced with anti-GBM sera. R...
Source: Journal of Autoimmunity - June 9, 2018 Category: Allergy & Immunology Authors: Zhen Y, Lee IJ, Finkelman FD, Shao WH Tags: J Autoimmun Source Type: research

Caveolin-1 regulates autophagy activity in thyroid follicular cells and is involved in Hashimoto's thyroiditis disease.
In this study, we examined Th1 cytokines and Cav-1 expression in HT thyroid tissues, determined the effects of interleukin-1beta (IL-1β) and interferon-gamma (IFN-γ) on Cav-1 and autophagy activity in TFC, and investigated the association between Cav-1 and autophagy activity in vitro. Our results indicate that higher levels of IL-1β and IFN-γ and lower levels of Cav-1 were expressed in thyroid tissues of HT patients than in those of normal controls. Cav-1 mRNA and protein levels were significantly decreased in TFC exposed to IL-1β and IFN-γ, accompanied by decreased expression of autophagy-related protein LC3B-II. In...
Source: Endocrine Journal - June 8, 2018 Category: Endocrinology Tags: Endocr J Source Type: research

Transcriptional activation of p21Waf1 contributes to suppression of HR by p53 in response to replication arrest induced by camptothecin.
Authors: Romanova LY, Mushinski F, Kovalchuk AL Abstract The inhibitory effect of p53 on homologous recombination (HR) is exerted through sequestration of replication protein A (RPA). Release of the p53/RPA complex in response to replication stress is crucially dependent on the phosphorylation status of both proteins and is required for efficient DNA repair by HR. Phosphorylation of RPA within its RPA2 subunit by cyclin-dependent kinases (CDK) is an early event in the replication stress response. Here we investigated the role of transcriptional activation of the p53 downstream target, p21Waf1, on RPA2 phosphorylati...
Source: Oncotarget - June 8, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

LncRNA FAS-AS1 promotes the degradation of extracellular matrix of cartilage in osteoarthritis.
CONCLUSIONS: The expression of FAS-AS1 was increased in osteoarthritis, and FAS-AS1 could be involved in the development of the disease by regulating the proliferation, apoptosis of chondrocytes and promoting the degradation of extracellular matrix. PMID: 29863238 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 8, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

The apoptotic effect of Zoledronic acid on the nasopharyngeal carcinoma cells via ROS mediated chloride channel activation.
This article is protected by copyright. All rights reserved. PMID: 29884989 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Pharmacology and Physiology - June 8, 2018 Category: Drugs & Pharmacology Authors: Wang L, Gao H, Yang X, Liang X, Tan Q, Chen Z, Zhao C, Gu Z, Yu M, Zheng Y, Huang Y, Zhu L, Jacob TJC, Wang L, Chen L Tags: Clin Exp Pharmacol Physiol Source Type: research

The immunoproteasome inhibitor ONX-0914 regulates inflammation and expression of contraction associated proteins in myometrium.
This article is protected by copyright. All rights reserved. PMID: 29883518 [PubMed - as supplied by publisher]
Source: European Journal of Immunology - June 8, 2018 Category: Allergy & Immunology Authors: Liong S, Lim R, Nguyen-Ngo C, Barker G, Parkington HC, Lappas M Tags: Eur J Immunol Source Type: research

Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara
Publication date: September 2018 Source:Redox Biology, Volume 18 Author(s): Joohee Lee, Kwangho Song, Eugene Huh, Myung Sook Oh, Yeong Shik Kim Oxidative stress plays a key role in neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Therefore, the nuclear factor-E2-related factor 2 (Nrf2), a key regulator of the antioxidative response, is considered to be important as a therapeutic target for neurodegenerative diseases. We investigated the underlying mechanism of Nrf2-mediated neuroprotective effects against oxidative stress in the PC12 cell line by 7β-(3-ethyl-cis-crotonoyloxy)-1α-(2-methylbutyrylo...
Source: Redox Biology - June 8, 2018 Category: Biology Source Type: research

Suppression of Pax2 Attenuates Allodynia and Hyperalgesia through ET-1 –ETAR–NFAT5 Signaling in a Rat Model of Neuropathic Pain
Publication date: 1 August 2018 Source:Neuroscience, Volume 384 Author(s): Lydia Wai Tai, Zhiqiang Pan, Liting Sun, Haobo Li, Pan Gu, Stanley Sau Ching Wong, Sookja K. Chung, Chi Wai Cheung Endothelin-1 (ET-1) and its receptors (ETAR/ETBR) emerge to be a key signaling axis in neuropathic pain processing and are recognized as new therapeutic targets. Yet, little is known on the functional regulation of ET-1 axis during neuropathic pain. Bioinformatics analysis indicated that paired box gene 2 (Pax2) or nuclear factor of activated T-cells 5 (NFAT5), two transcription factors involved in the modulation of neurotransmission, ...
Source: Neuroscience - June 8, 2018 Category: Neuroscience Source Type: research

lncRNA NEAT1 competes against let-7a to contribute to non-small cell lung cancer proliferation and metastasis.
In conclusion, NEAT1 regulates lung cancer cell progression by competing endogenous RNA network of NEAT1/let-7a/IGF2. Our findings provide a novel insight into the biological function of NEAT1 in lung cancer and NEAT1 could be a potential therapeutic target for lung cancer. PMID: 29864936 [PubMed - in process]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - June 7, 2018 Category: Drugs & Pharmacology Authors: Qi L, Liu F, Zhang F, Zhang S, Lv L, Bi Y, Yu Y Tags: Biomed Pharmacother Source Type: research

Autophagy inhibition attenuates the induction of anti-inflammatory effect of catalpol in liver fibrosis.
In conclusion, catalpol relieved liver fibrosis mainly by inhibiting inflammation, and autophagy inhibition attenuated the catalpol-induced anti-inflammatory effect on liver fibrosis. PMID: 29864907 [PubMed - in process]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - June 7, 2018 Category: Drugs & Pharmacology Authors: Liu Z, Zhu P, Zhang L, Xiong B, Tao J, Guan W, Li C, Chen C, Gu J, Duanmu J, Zhang W Tags: Biomed Pharmacother Source Type: research

LncRNA SNHG1 regulates the differentiation of Treg cells and affects the immune escape of breast cancer via regulating miR-448/IDO.
CONCLUSION: Interference SNHG1 could inhibit the differentiation of Treg cells by promoting miR-448 expression and reducing IDO level, thereby impeding the immune escape of BC. PMID: 29886172 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - June 7, 2018 Category: Biochemistry Authors: Pei X, Wang X, Li H Tags: Int J Biol Macromol Source Type: research

Osteopontin attenuates inflammation via JAK2/STAT1 pathway in hyperglycemic rats after intracerebral hemorrhage.
In conclusion, rOPN attenuated ICH-induced brain inflammation in hyperglycemic rats, leading to attenuation of brain edema and improving neurological functions. Effects of rOPN were mediated at least partly by integrin-β1 induced inhibition of JAK2/STAT1 pathway. PMID: 29885817 [PubMed - as supplied by publisher]
Source: Neuropharmacology - June 7, 2018 Category: Drugs & Pharmacology Authors: Gong L, Manaenko A, Fan R, Huang L, Enkhjargal B, McBride D, Ding Y, Tang J, Xiao X, Zhang JH Tags: Neuropharmacology Source Type: research

Pontin Acts as a Potential Biomarker for Poor Clinical Outcome and Promotes Tumor Invasion in Hilar Cholangiocarcinoma.
Abstract Hilar cholangiocarcinoma (HC) is a devastating malignancy that carries a poor overall prognosis. As a member of the AAA+ superfamily, Pontin becomes highly expressed in several malignant tumors, which contributes to tumor progression and influences tumor prognosis. In our research, Pontin expression in tumor specimens resected from 86 HC patients was detected by immunohistochemistry. Interestingly, high expression of Pontin was significantly associated with lymph node metastasis (p = 0.011) and tumor node metastasis (TNM) stage (p = 0.005). The Kaplan-Meier overall survival rate and multivariate analyses ...
Source: Biomed Res - June 6, 2018 Category: Research Authors: Sun Q, Li F, Yu S, Zhang X, Shi F, She J Tags: Biomed Res Int Source Type: research

Activation of Sonic Hedgehog Signaling Is Associated with Human Osteosarcoma Cells Radioresistance Characterized by Increased Proliferation, Migration, and Invasion.
CONCLUSIONS Activation of Shh signaling was involved in radioresistance of OS cells. Blocking this signaling can impair the radioresistance capacity of OS cells. PMID: 29864766 [PubMed - in process]
Source: Medical Science Monitor - June 6, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells
Source: OncoTargets and Therapy - June 6, 2018 Category: Cancer & Oncology Tags: OncoTargets and Therapy Source Type: research

The combinational effect of E6/E7 siRNA and anti-miR-182 on apoptosis induction in HPV16-positive cervical cells
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Source: Artificial Cells, Blood Substitutes, and Biotechnology - June 6, 2018 Category: Biotechnology Authors: Hamidreza Javadi Abbas Sahebghadam Lotfi Saman Hosseinkhani Hossein Mehrani Jafar Amani Zahra Soheila Soheili Zahra Hojati Mehdi Kamali Source Type: research

GSE115270 Differential expression profiling of the TamR cells following AR knockdown
In this study, we sought to characterize the functional consequences of AR knockdown using a siRNA-mediated approach in a tamoxifen-resistant (TamR) derivative of MCF7 breast cancer cell line.
Source: GEO: Gene Expression Omnibus - June 5, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Inhibition of miR-135b by SP-1 promotes hypoxia-induced vascular endothelial cell injury via HIF-1 α.
In conclusion, the SP-1/miR-135b/HIF-1α axis may play a critical role in hypoxia-induced vascular endothelial injury. Our study thus provides novel insights into the role of this transcription factor and miRNAs in the pathogenesis of hypoxia-induced cardiac dysfunctions. PMID: 29883713 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - June 5, 2018 Category: Cytology Authors: Yang S, Yin J, Hou X Tags: Exp Cell Res Source Type: research

Neurotrophin-3 provides neuroprotection via TrkC receptor dependent pErk5 activation in a rat surgical brain injury model.
CONCLUSIONS: Topical application of NT-3 reduced brain edema, BBB permeability and improved neurological function after SBI. The protective effect of NT-3 was possibly mediated via TrkC dependent activation of Erk5. PMID: 29883578 [PubMed - as supplied by publisher]
Source: Experimental Neurology - June 5, 2018 Category: Neurology Authors: Akyol O, Sherchan P, Yilmaz G, Reis C, Ho WM, Wang Y, Huang L, Solaroglu I, Zhang JH Tags: Exp Neurol Source Type: research

Role of PERK-eIF2 α signaling pathway in fetal male rats with hypospadias induced by di-n-butyl phthalate
This study aims to explore the role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by maternal exposure to di-n-butyl phthalate (DBP). DBP was used to treat pregnant SD rats by gastric intubation from gestation day (GD) 14–18 to construct a hypospadias rat model. The amount, weight, anogenital distance (AGD), and hypospadias incidence of rats were recorded and the genital tubercle (GT) of fetal male rats was collected on GD 19. Western blotting was performed to detect the expressions of PERK-eIF2α pathway- and autophagy-related proteins, and cell apoptosis was detected using TUNEL method. T...
Source: The Kaohsiung Journal of Medical Sciences - June 5, 2018 Category: Universities & Medical Training Source Type: research

Interaction of S100A1 with LATS1 promotes cell growth through regulation of the Hippo pathway in hepatocellular carcinoma.
In conclusion, the results of the present study indicated that S100A1 functions as an oncogene and may be a biomarker for the prognosis of patients with HCC. S100A1 exerted its oncogenic function by interacting with LATS1 and activating YAP. S100A1 may serve as a target for novel therapies in HCC. PMID: 29901195 [PubMed - as supplied by publisher]
Source: International Journal of Oncology - June 5, 2018 Category: Cancer & Oncology Authors: Guo Q, Wang J, Cao Z, Tang Y, Feng C, Huang F Tags: Int J Oncol Source Type: research

MicroRNA-155 suppresses the catabolic effect induced by TNF- α and IL-1β by targeting C/EBPβ in rat nucleus pulposus cells.
CONCLUSIONS: miR-155 is a sustainable factor for intervertebral disc and suppresses the expression of catabolic genes induced by TNF-α and IL-1β by targeting C/EBPβ in rat NP cells. PMID: 29852820 [PubMed - as supplied by publisher]
Source: Connective Tissue Research - June 4, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

CKIP-1 suppresses odontoblastic differentiation of dental pulp stem cells via BMP2 pathway and can interact with NRP1.
CONCLUSIONS: This work provides data that it advocates a novel perception on odontoblastic differentiation of DPSCs. Therefore, inhibiting the expression of CKIP-1 may be of great significance to the development of dental caries. PMID: 29852799 [PubMed - as supplied by publisher]
Source: Connective Tissue Research - June 4, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Isoquercitrin promotes the osteogenic differentiation of osteoblasts and BMSCs via the RUNX2 or BMP-pathway.
Authors: Li M, Zhang C, Li X, Lv Z, Chen Y, Zhao J Abstract Isoquercitrin is widely present in fruits, vegetables and medicinal herbs. As a natural phytoestrogen, isoquercitrin has been considered a possible osteoporosis prevention option to avoid the risk of hormone therapy. In this work, we studied the effects of isoquercitrin on the cell proliferation and osteogenic differentiation of osteoblasts and bone mesenchymal stem cells (BMSCs), as well as the molecular mechanism of isoquercitrin action. The cell proliferation of osteoblasts and BMSCs was promoted by isoquercitrin at low concentrations. High concentratio...
Source: Connective Tissue Research - June 4, 2018 Category: Research Tags: Connect Tissue Res Source Type: research

Targeting PIM kinase as a therapeutic strategy in human hepatoblastoma.
Authors: Stafman LL, Mruthyunjayappa S, Waters AM, Garner EF, Aye JM, Stewart JE, Yoon KJ, Whelan K, Mroczek-Musulman E, Beierle EA Abstract Increasing incidence coupled with poor prognosis and treatments that are virtually unchanged over the past 20 years have made the need for the development of novel therapeutics for hepatoblastoma imperative. PIM kinases have been implicated as drivers of tumorigenesis in multiple cancers, including hepatocellular carcinoma. We hypothesized that PIM kinases, specifically PIM3, would play a role in hepatoblastoma tumorigenesis and that PIM kinase inhibition would affect hepatobl...
Source: Oncotarget - June 4, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

MYB induces the expression of the oncogenic corepressor SKI in acute myeloid leukemia.
Authors: Frech M, Teichler S, Feld C, Bouchard C, Berberich H, Sorg K, Mernberger M, Bullinger L, Bauer UM, Neubauer A Abstract Acute myeloid leukemia (AML) arises through clonal expansion of transformed myeloid progenitor cells. The SKI proto-oncogene is highly upregulated in different solid tumors and leukemic cells, but little is known about its transcriptional regulation during leukemogenesis. MYB is an important hematopoietic transcription factor involved in proliferation as well as differentiation and upregulated in most human acute leukemias. Here, we find that MYB protein binds within the regulatory region ...
Source: Oncotarget - June 4, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5
Heat-shock molecular chaperone proteins (Hsps) promote the loading of small interfering RNA (siRNA) onto RNA interference (RNAi) effector complexes. While the RNAi process is coupled with heterochromatin assem...
Source: Epigenetics and Chromatin - June 4, 2018 Category: Genetics & Stem Cells Authors: Kosuke Okazaki, Hiroaki Kato, Tetsushi Iida, Kaori Shinmyozu, Jun-ichi Nakayama, Yota Murakami and Takeshi Urano Tags: Research Source Type: research

Cancers, Vol. 10, Pages 180: KIAA0100 Modulates Cancer Cell Aggression Behavior of MDA-MB-231 through Microtubule and Heat Shock Proteins
Spetzler The KIAA0100 gene was identified in the human immature myeloid cell line cDNA library. Recent studies have shown that its expression is elevated in breast cancer and associated with more aggressive cancer types as well as poor outcomes. However, its cellular and molecular function is yet to be understood. Here we show that silencing KIAA0100 by siRNA in the breast cancer cell line MDA-MB-231 significantly reduced the cancer cells’ aggressive behavior, including cell aggregation, reattachment, cell metastasis and invasion. Most importantly, silencing the expression of KIAA0100 particularly sensit...
Source: Cancers - June 4, 2018 Category: Cancer & Oncology Authors: Zhenyu Zhong Vaishali Pannu Matthew Rosenow Adam Stark David Spetzler Tags: Article Source Type: research

Lead Neurotoxicity on Human Neuroblastoma Cell Line SH-SY5Y is Mediated via Transcription Factor EGR1/Zif268 Induced Disrupted in Scherophernia-1 Activation.
Abstract Lead (Pb2+) is a well-known type of neurotoxin and chronic exposure to Pb2+ induces cognition dysfunction. In this work, the potential role of early growth response gene 1 (EGR1) in the linkage of Pb2+ exposure and disrupted in scherophernia-1 (DISC1) activity was investigated. Human neuroblastoma cell line SH-SY5Y was subjected to different concentrations of lead acetate (PbAc) to determine the effect of Pb2+ exposure on the cell viability, apoptosis, and activity of EGR1 and DISC1. Then the expression of EGR1 in SH-SY5Y cells was knocked down with specific siRNA to assess the function of EGR1 in Pb2+ in...
Source: Neurochemical Research - June 4, 2018 Category: Neuroscience Authors: You Y, Peng B, Ben S, Hou W, Sun L, Jiang W Tags: Neurochem Res Source Type: research

The small non-coding RNA response to virus infection in the < i > Leishmania < /i > vector < i > Lutzomyia longipalpis < /i >
by Fl ávia Viana Ferreira, Eric Roberto Guimarães Rocha Aguiar, Roenick Proveti Olmo, Karla Pollyanna Vieira de Oliveira, Emanuele Guimarães Silva, Maurício Roberto Viana Sant'Anna, Nelder de Figueiredo Gontijo, Erna Geessien Kroon, Jean Luc Imler, João Trindade Marques Sandflies are well known vectors forLeishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA)...
Source: PLoS Neglected Tropical Diseases - June 4, 2018 Category: Tropical Medicine Authors: Fl ávia Viana Ferreira Source Type: research

Osteopontin Expression Is Associated with the Poor Prognosis in Patients with Locally Advanced Esophageal Squamous Cell Carcinoma Receiving Preoperative Chemoradiotherapy.
Conclusions: Our results suggest that osteopontin may be a potential therapeutic target for patients with ESCC treated with preoperative chemoradiotherapy. PMID: 29854808 [PubMed - in process]
Source: Biomed Res - June 3, 2018 Category: Research Authors: Chiu TJ, Lu HI, Chen CH, Huang WT, Wang YM, Lin WC, Li SH Tags: Biomed Res Int Source Type: research

TLR4/PKC α/occludin signaling pathway may be related to blood‑brain barrier damage.
TLR4/PKCα/occludin signaling pathway may be related to blood‑brain barrier damage. Mol Med Rep. 2018 May 16;: Authors: Tang Z, Guo D, Xiong L, Wu B, Xu X, Fu J, Kong L, Liu Z, Xie C Abstract Abnormal blood-brain barrier (BBB) is a common pathological feature in brain damage. In the present study, a brain microvascular endothelial cell (BMEC) model was established to determine the role of the toll‑like receptor 4 (TLR4)/protein kinase Cα (PKCα)/occludin signaling pathway in BBB dysfunction. Three small interfering (si)RNAs directed against PKCα were designed to investigate the molecular mechanis...
Source: Molecular Medicine Reports - June 2, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Napsin A is negatively associated with EMT ‑mediated EGFR‑TKI resistance in lung cancer cells.
Napsin A is negatively associated with EMT‑mediated EGFR‑TKI resistance in lung cancer cells. Mol Med Rep. 2018 May 25;: Authors: Zhou L, Lv X, Yang J, Zhu Y, Wang Z, Xu T Abstract Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKI) have been used as a standard therapy for patients with lung cancer with EGFR‑activating mutations. Epithelial‑mesenchymal transition (EMT) has been reported to be associated with the development of EGFR‑TKI resistance, which limits the clinical efficacy of EGFR‑TKI. Therefore, investigating the resistance‑associated mechanism is required i...
Source: Molecular Medicine Reports - June 2, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Ginsenoside Rb1 increases macrophage phagocytosis through p38 mitogen-activated protein kinase/Akt pathway
Conclusion These results suggest that ginsenoside Rb1 enhances the phagocytic capacity of macrophages for bacteria via activation of the p38/Akt pathway. Rb1 may be a useful pharmacological adjuvant for the treatment of bacterial infections in clinically relevant conditions.
Source: Journal of Ginseng Research - June 2, 2018 Category: Complementary Medicine Source Type: research

Silver nanoparticle-induced hormesis of astroglioma cells: A Mu-2-related death-inducing protein-orchestrated modus operandi.
In this study, we investigated whether silver nanoparticles (AgNPs) induce hormesis in astroglioma cells and the possible involvement of MuD in AgNP-induced hormesis. AgNPs exhibited cytotoxic effects on cell proliferation in a dose-dependent manner and increased MuD expression was observed during AgNP-induced astroglioma hormesis. Studies using MuD-knockout cells and MuD siRNA transfection showed that MuD might influence cell viability upon AgNP treatment. In addition, apoptotic cell population and production of reactive oxygen species in the absence of MuD were significantly increased. The phosphorylation of two mitogen-...
Source: International Journal of Biological Macromolecules - June 2, 2018 Category: Biochemistry Authors: Choi JH, Min WK, Gopal J, Lee YM, Muthu M, Chun S, Oh JW Tags: Int J Biol Macromol Source Type: research

Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2.
Authors: Zhou H, Ni Z, Li T, Su L, Zhang L, Liu N, Shi Y Abstract Gastric intestinal metaplasia (IM) induced by bile acid is a precancerous lesion of gastric adenocarcinoma and is associated with the expression of caudal-related homeobox 2 (CDX2). In the present study, the role of farnesoid X receptor (FXR) on the regulation of CDX2 in gastric cells was investigated and the underlying molecular mechanisms were examined. Human gastric cell lines were treated with chenodeoxycholic acid (CDCA) or FXR agonist GW4064. Cells were treated with CDCA in the presence or absence of the FXR antagonist or FXR siRNA transfection...
Source: Oncology Letters - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Expression of cold-inducible RNA-binding protein (CIRP) in renal cell carcinoma and the effect of CIRP downregulation cell proliferation and chemosensitivity to gemcitabine.
Authors: Zhou KW, Jiang K, Zhu W, Weng G Abstract The aim of the present study was to investigate the expression of cold-inducible RNA-binding protein (CIRP) in renal cell carcinoma (RCC) and to determine the effects of downregulation of CIRP on cell proliferation and chemosensitivity to gemcitabine. The expression of CIRP was detected by western blot analysis, quantitative polymerase chain reaction and immunohistochemistry (IHC) in 17 RCC and peri-cancerous tissue samples. Subsequently, the RCC 786-0 cell line was selected in order to investigate the function of CIRP using RNA interference (RNAi) technology, which...
Source: Oncology Letters - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Focal adhesion kinase promotes progression and predicts poor clinical outcomes in patients with osteosarcoma.
Authors: Gu HJ, Zhou B Abstract Osteosarcoma (OS) is a fatal form of musculoskeletal tumor that commonly leads to pulmonary metastatic disease. Traditional therapies such as surgery and chemotherapy are not effective treatment modalities in certain patients with OS; therefore, identifying the molecular mechanism of OS is imperative for the development of novel therapeutics. Previous studies have reported that focal adhesion kinase (FAK) is associated with numerous types of human malignancies. Therefore, in order to investigate the biological function of FAK in OS, the present study examined the expression levels of...
Source: Oncology Letters - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Role of hepcidin and iron metabolism in the onset of prostate cancer.
Authors: Zhao B, Li R, Cheng G, Li Z, Zhang Z, Li J, Zhang G, Bi C, Hu C, Yang L, Lei Y, Wang Q Abstract The present study aimed to understand the roles of hepcidin and iron metabolism in the onset of prostate cancer. The prostate cancer LNCap, PC3 and DU145 cell lines were transfected with small interfering RNA (siRNA) targeting hepcidin to knockdown hepcidin expression in LNCap, PC3 and DU145 cells. The expression levels of hepcidin in prostate cancer and normal prostate RWPE-1cells were detected by western blot analysis. Exogenous hepcidin was added into the hepcidin-silenced cell lines. Intracellular iron level...
Source: Oncology Letters - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

RAD52 motif ‑containing protein 1 promotes non‑small cell lung cancer cell proliferation and survival via cell cycle regulation.
This study aimed to investigate the role of RDM1 in the progression of non‑small cell lung cancer (NSCLC). We found elevated RDM1 mRNA and protein expression in NSCLC tissues and cell lines compared to levels in normal lung cells. RDM1 protein expression in lung cancer tissues was found to correlate with tumor size, histological differentiation, lymph node metastasis and tumor‑node‑metastasis (TNM) stage. Knockdown of the RDM1 gene with siRNA significantly reduced the cellular proliferation rate and increased apoptosis in the human NSCLC cell line, NCI‑H1299. Compared to wild‑type NCI‑H1299 cells, RDM1 knockdow...
Source: Oncology Reports - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Montelukast, a cysteinyl leukotriene receptor antagonist, inhibits the growth of chronic myeloid leukemia cells through apoptosis.
Authors: Zovko A, Yektaei-Karin E, Salamon D, Nilsson A, Wallvik J, Stenke L Abstract The clinical outcome for patients with chronic myeloid leukemia (CML) has improved significantly with the introduction of tyrosine kinase inhibitors (TKIs). However, their curative potential appears limited, probably as a consequence of TKI-resistant leukemic stem cells (LSCs) that persist as a result of aberrant pathways independent of the well-established oncoprotein Bcr-Abl. One such pathway involves signaling through leukotrienes (LTs), bioactive compounds that have been suggested to play a role in several other malignancies. ...
Source: Oncology Reports - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR ‑218 promotes apoptosis of SW1417 human colon cancer cells by targeting c‑FLIP.
miR‑218 promotes apoptosis of SW1417 human colon cancer cells by targeting c‑FLIP. Oncol Rep. 2018 May 23;: Authors: Meng Q, Chen Y, Lian B, Shang Y, Yang H Abstract MicroRNAs (miRNAs) are suggested to act as either tumor oncogenes or tumor suppressors in different types of cancer. miRNA‑218 (miR‑218) is a type of short, non-coding RNA which is involved in gastric cancer development. In the present study, we evaluated the functions of miR‑218 in SW1417 human colon cancer cells and its potential mechanisms. Following overexpression of miR‑218 in human colon cancer cells, cell viability was d...
Source: Oncology Reports - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5.
Authors: Angelucci C, D'Alessio A, Iacopino F, Proietti G, Di Leone A, Masetti R, Sica G Abstract The influence of cell membrane fluidity on cancer progression has been established in different solid tumors. We previously reported that "cancer-associated fibroblasts" (CAFs) induced epithelial-mesenchymal transition and increased cell membrane fluidity and migration in poorly (MCF-7) and highly invasive (MDA-MB-231) breast cancer cells. We also found that the membrane fluidity regulating enzyme stearoyl-CoA desaturase 1 (SCD1) was upregulated in tumor cells co-cultured with CAFs and established its essential role fo...
Source: Oncotarget - June 1, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Inhibitory Effects of a Novel PPAR- γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells.
Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells. PPAR Res. 2018;2018:5346272 Authors: Parvin R, Noro E, Saito-Hakoda A, Shimada H, Suzuki S, Shimizu K, Miyachi H, Yokoyama A, Sugawara A Abstract Although therapeutic effects of the peroxisome proliferator-activated receptor gamma (PPAR-γ) agonists rosiglitazone and pioglitazone against Cushing's disease have been reported, their effects are still controversial and inconsistent. We therefore examined the effects of a novel PPAR-γ agonist, MEKT1, on Pomc expression/ACTH secretion using murine corticot...
Source: PPAR Research - June 1, 2018 Category: Genetics & Stem Cells Tags: PPAR Res Source Type: research

Muramyl Dipeptide Induces Reactive Oxygen Species Generation Through the NOD2/COX-2/NOX4 Signaling Pathway in Human Umbilical Vein Endothelial Cells
Abstract: Vascular endothelium dysfunction caused by oxidative stress accelerates the pathologic process of cardiovascular diseases. NOD2, an essential receptor of innate immune system, has been demonstrated to play a critical role in atherosclerosis. Here, the aim of our study was to investigate the effect and underlying molecular mechanism of muramyl dipeptide (MDP) on NOX4-mediated reactive oxygen species (ROS) generation in human umbilical vein endothelial cells (HUVECs). The 2,7-dichlorofluorescein diacetate staining was to measure the intracellular ROS level and showed MDP-promoted ROS production in a time- and dos...
Source: Journal of Cardiovascular Pharmacology - June 1, 2018 Category: Cardiology Tags: Original Article Source Type: research

GSE85818 Nicotiana tabacum degradome analysis using high definition adapters proves an efficient targeting method against the Tomato leaf curl New Delhi virus using artificial ta-siRNAs PARE
Contributors : Archana Singh ; Irina Mohorianu ; Tamas Dalmay ; Sunil Mukherjee ; Indranil DasguptaSeries Type : Expression profiling by high throughput sequencingOrganism : Nicotiana tabacumRNA interference (RNAi) is a conserved, RNA-mediated, regulatory mechanism in eukaryotes. In plants, it plays an important role in growth, development and resistance against viral infections. As a counter-defence, plant viruses, e.g. geminiviruses, encode RNAi suppressors, such as AC2, AC4 and AV2. To obtain Nicotiana tabacum virus resistant plants against Tomato leaf curl New Delhi virus (ToLCNDV), we employ the biogenesis pathway of ...
Source: GEO: Gene Expression Omnibus - June 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Nicotiana tabacum Source Type: research

GSE85816 Nicotiana tabacum degradome analysis using high definition adapters proves an efficient targeting method against the Tomato leaf curl New Delhi virus using artificial ta-siRNAs sRNA-seq
Contributors : Archana Singh ; Irina Mohorianu ; Tamas Dalmay ; Sunil Mukherjee ; Indranil DasguptaSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Nicotiana tabacumRNA interference (RNAi) is a conserved, RNA-mediated, regulatory mechanism in eukaryotes. In plants, it plays an important role in growth, development and resistance against viral infections. As a counter-defence, plant viruses, e.g. geminiviruses, encode RNAi suppressors, such as AC2, AC4 and AV2. To obtain Nicotiana tabacum virus resistant plants against Tomato leaf curl New Delhi virus (ToLCNDV), we employ the biogenesis pathway...
Source: GEO: Gene Expression Omnibus - June 1, 2018 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Nicotiana tabacum Source Type: research