Query: siRNA

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Total 13804 results found since Jan 2013.

Lipoxin A4 Ameliorates Lipopolysaccharide-Induced A549 Cell Injury through Upregulation of N-myc Downstream-Regulated Gene-1.
Conclusion: Our research uncovered a critical role of NDRG1 in LXA4 alleviation of LPS-induced A549 cell injury through mediating PI3K signaling to restore ENaC expression. PMID: 29786049 [PubMed - in process]
Source: Chinese Medical Journal - May 24, 2018 Category: General Medicine Authors: Zhang JZ, Liu ZL, Zhang YX, Lin HJ, Zhang ZJ Tags: Chin Med J (Engl) Source Type: research

Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study.
Conclusions: Both hydrochloric acid and deoxycholic acid could suppress Notch signaling pathway in esophageal epithelial cells, and inhibited Notch signaling has important functions in the development of Barrett's esophagus. PMID: 29785394 [PubMed - in process]
Source: Canadian Journal of Gastroenterology - May 24, 2018 Category: Gastroenterology Tags: Can J Gastroenterol Hepatol Source Type: research

JCAD Gene at the 10p11 Coronary Artery Disease Locus Regulates Hippo Signaling in Endothelial Cells.
CONCLUSIONS: Our results show that JCAD negatively regulates Hippo signaling in endothelial cells and we suggest that JCAD contributes to atherosclerosis by mediating YAP activity and contributing to endothelial dysfunction. PMID: 29794114 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - May 24, 2018 Category: Cardiology Authors: Jones PD, Kaiser MA, Ghaderi Najafabadi M, Koplev S, Zhao Y, Douglas G, Kyriakou T, Andrews S, Rajmohan R, Watkins H, Channon KM, Ye S, Yang X, Björkegren JLM, Samani NJ, Webb TR Tags: Arterioscler Thromb Vasc Biol Source Type: research

USP10 regulates the stability of the EMT-transcription factor Slug/SNAI2.
Abstract Epithelial-to-mesenchymal transition (EMT) is a fundamental mechanism governing the switch of cells from an epithelial to a motile mesenchymal-like state. This transdifferentiation is regulated by key transcription factors, including Slug. The stability and function of Slug can be regulated by multiple mechanisms, including ubiquitin-mediated post-translational modifications. Here, by using a genome wide siRNA screen for human deubiquitinating enzymes (DUBs), we identified USP10 as a deubiquitinase for Slug in cancer cells. USP10 interacts with Slug and mediates its degradation by the proteasome. Importan...
Source: Biochemical and Biophysical Research communications - May 24, 2018 Category: Biochemistry Authors: Ouchida AT, Kacal M, Zheng A, Ambroise G, Zhang B, Norberg E, Vakifahmetoglu-Norberg H Tags: Biochem Biophys Res Commun Source Type: research

Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF- κB pathway.
Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF-κB pathway. Acta Pharmacol Sin. 2018 May 24;: Authors: Liu ZC, Wang ZL, Huang CY, Fu ZJ, Liu Y, Wei ZC, Liu SG, Ma C, Shen JL, Duan DD Abstract Lower back pain (LBP) is the most common disease in orthopedic clinics world-wide. A classic fangji of traditional Chinese medicine, duhuo jisheng decoction (DHJSD), has been proven clinically effective for LBP but its therapeutic mechanisms remain unclear. We hypothesized that DHJSD might relieve LBP th...
Source: Acta Pharmacologica Sinica - May 24, 2018 Category: Drugs & Pharmacology Authors: Liu ZC, Wang ZL, Huang CY, Fu ZJ, Liu Y, Wei ZC, Liu SG, Ma C, Shen JL, Duan DD Tags: Acta Pharmacol Sin Source Type: research

siRNA release from gold nanoparticles by nanosecond pulsed laser irradiation and analysis of the involved temperature increase
Journal of Biophotonics, EarlyView.
Source: Journal of Biophotonics - May 24, 2018 Category: Physics Authors: Florian Rudnitzki , Susanne Feineis , Ramtin Rahmanzadeh , Elmar Endl , Johanna Lutz , J ürgen Groll , Gereon Hüttmann Source Type: research

Neuralized1a regulates asymmetric division in mouse Lewis lung carcinoma cells
Publication date: 1 August 2018 Source:Life Sciences, Volume 206 Author(s): Liangsheng Kong, Jingyuan Li, Yongli Liu, Zhiwei Sun, Shixia Zhou, Junling Tang, Ting Ye, Jianyu Wang, H. Rosie Xing Asymmetric division (ASD), the unique characteristic of normal stem cells, is regarded as a stemness marker when applied to the study of cancer stem cells (CSCs). However, the role of ASD in the self-renewal of CSCs and its regulation remain largely unknown. Here, we first established a mouse Lewis lung carcinoma CSC cell line that could undergo asymmetric division (LLC-ASD cells) derived from the parental mouse Lewis lung carcinoma...
Source: Life Sciences - May 24, 2018 Category: Biology Source Type: research

The preclinical characterization of 18FhGTS13 for imaging of xC- transporter activity
Conclusion: [18F]hGTS13 is a novel radiotracer for measuring xC- transporter activity. It showed high and specific uptake in cell assays with A549 and H460 cells. [18F]hGTS13 showed significantly lower uptake in activated T cells compared to [18F]FSPG. PET/CT imaging of [18F]hGTS13 in H460 tumor bearing rats revealed significantly higher tumor uptake than [18F]FSPG.
Source: Journal of Nuclear Medicine - May 23, 2018 Category: Nuclear Medicine Authors: Beinat, C., Gowrishanker, G., Shen, B., Alam, I., Robinson, E., Ilovich, O., Koglin, N., Schmitt-Willich, H., Berndt, M., Mueller, A., Zerna, M., Srinivasan, A., Gambhir, S. Tags: Preclinical Probes for Oncology III Source Type: research

Let ‑7d inhibits colorectal cancer cell proliferation through the CST1/p65 pathway.
Let‑7d inhibits colorectal cancer cell proliferation through the CST1/p65 pathway. Int J Oncol. 2018 May 23;: Authors: Jiang J, Liu HL, Tao L, Lin XY, Yang YD, Tan SW, Wu B Abstract Cystatin SN (cystatin 1, CST1) is a member of the cystatin superfamily which inhibits the proteolytic activity of cysteine proteases. CST1 is a tumor biomarker that provides useful information for the diagnosis of esophageal, gastric and colorectal carcinomas. MicroRNAs (miRNAs or miRs) play an important role in tumor cell proliferation. However, the exact role of let‑7d and CST1 in colon cancer remains unknown. The a...
Source: International Journal of Oncology - May 23, 2018 Category: Cancer & Oncology Authors: Jiang J, Liu HL, Tao L, Lin XY, Yang YD, Tan SW, Wu B Tags: Int J Oncol Source Type: research

Effects of RNAi Silencing CITED1 Gene on Biological Function of Thyroid Carcinoma Cell Line K1
Conclusions: CITED1 gene may affect the occurrence and development of thyroid papillary cancer by promoting tumor cell proliferation and migration,and inhibiting apoptosis. Key words: CITED1, K1, RNAi, apoptosis, proliferation
Source: Journal of Nuclear Medicine - May 23, 2018 Category: Nuclear Medicine Authors: Pang, H. Tags: Educational Exhibits Source Type: research

Effect of IDH3{alpha} on glucose uptake in lung adenocarcinoma
Conclusion: High expression of IDH3α in lung adenocarcinoma patients showed higher glucose uptake than low expression ones. IDH3α affect glucose uptake of lung cancer through AKT-GLUT1 pathway, the down-regulation of IDH3α can reduce the generation of lactic acid in tumor cells.
Source: Journal of Nuclear Medicine - May 23, 2018 Category: Nuclear Medicine Authors: Du, B., Li, X., Li, Y. Tags: Basic Science Posters (Oncology) Source Type: research

Complex roads from genotype to phenotype in dilated cardiomyopathy: scientific update from the Working Group of Myocardial Function of the European Society of Cardiology
This article is part of the Mini Review Series from the Varenna 2017 meeting of the Working Group of Myocardial Function of the European Society of Cardiology.
Source: Cardiovascular Research - May 23, 2018 Category: Cardiology Source Type: research

The imaging research of chemokine receptor 4
Conclusions: With the bifunctional chelator HYNIC and SnCl2,the CXCR4 siRNA molecules probe that labeled with 99mTc was constructed successfully. The molecular probe has the better labeling efficiency, stability and interference activity.The 99mTc-HYNIC -siRNA1026 could specificity accumulated in the tissue with CXCR4overexpression Key words:CXCR4; siRNA; T-47D cells; 99mTc
Source: Journal of Nuclear Medicine - May 23, 2018 Category: Nuclear Medicine Authors: Xu, P. Tags: Technologist Poster Session Source Type: research

Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes.
Abstract Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyt...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2018 Category: Biochemistry Authors: Su Y, Tian H, Wei L, Fu G, Sun T Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

cAMP/PKA/EGR1 signaling mediates the molecular mechanism of ethanol-induced inhibition of placental 11 β-HSD2 expression.
This study aimed to investigate the molecular mechanism of the prenatal ethanol exposure (PEE)-induced inhibition of placental 11β-HSD2 expression. Pregnant Wistar rats were intragastrically administered ethanol (4 g/kg d) from gestational days 9 to 20. The levels of maternal and fetal serum corticosterone and placental 11β-HSD2-related gene expression were analyzed. Furthermore, we investigated the mechanism of reduced placental 11β-HSD2 expression induced by ethanol treatment (15-60 mM) in HTR-8/SVneo cells. In vivo, PEE decreased fetal body weights and increased maternal and fetal serum corticosterone and early...
Source: Toxicology and Applied Pharmacology - May 23, 2018 Category: Toxicology Authors: Yu L, Zhou J, Zhang G, Huang W, Pei L, Lv F, Zhang Y, Zhang W, Wang H Tags: Toxicol Appl Pharmacol Source Type: research

GSE114692 Effect of LIMD1 knockdown on gene expression in Human Bronchial Epithelial Cells (HBECs)
Contributors : Daniel E Foxler ; Katherine S Bridge ; John G Foster ; Paul Grevitt ; Sean Curry ; Kunal M Shah ; Kathryn M Davidson ; Ai Nagano ; Emanuela Gadaleta ; Hefin I Rhys ; Paul T Kennedy ; Miguel A Hermida ; Ting-Yu Chang ; Peter E Shaw ; Louise E Reynolds ; Tristan R McKay ; Hsei-Wei Wang ; Paulo S Ribeiro ; Michael J Plevin ; Dimitris Lagos ; Nicholas R Lemoine ; Prabhakar Rajan ; Trevor A Graham ; Claude Chelala ; Kairbaan M Hodivala-Dilke ; Ian Spendlove ; Tyson V SharpSeries Type : Expression profiling by arrayOrganism : Homo sapiensHBECs were untreated, treated with scrambled siRNA (80nM) or LIMD1 targeting ...
Source: GEO: Gene Expression Omnibus - May 22, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Hypoxia ‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α.
Hypoxia‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α. Int J Mol Med. 2018 May 22;: Authors: Zhang Z, Li P, Wang Y, Yan H Abstract The placenta initially develops in a low‑oxygen environment up to week 8‑10 of gestation, and a low oxygen level is a critical factor in the regulation of trophoblast migration and invasion. CXC chemokine receptor 4 (CXCR4) is transcriptionally activated by hypoxia in cancer cells. However, whether CXCR4 is involved in hypoxia‑inducible factor (HIF)‑1α‑dependent trophoblastic migration and invasion i...
Source: International Journal of Molecular Medicine - May 22, 2018 Category: Molecular Biology Authors: Zhang Z, Li P, Wang Y, Yan H Tags: Int J Mol Med Source Type: research

The expression and role of lncRNA AX800134 in hepatitis B virus-related hepatocellular carcinoma
In this study, we validated the upregulated expression of AX800134 in HBV-positive HCC compared with HBV-negative HCC. Furthermore, we found that HBV X protein (HBx) directly triggered AX800134 expression in human hepatoma HepG2 cells. Pro-inflammatory cytokine TNF α also induced AX800134 upregulation in HBx-expressing HepG2 cells, which could be reversed by reactive oxygen species (ROS) scavenger pyrrolidine dithiocarbamate (PDTC). Additionally, silencing AX800134 with siRNA interference remarkably inhibited the growth and invasion of HBx-expressing HepG2 ce lls. AX800134 antagonism also enhanced spontaneous apoptosis of...
Source: Virus Genes - May 22, 2018 Category: Genetics & Stem Cells Source Type: research

Down-regulation of KIF2A inhibits gastric cancer cell invasion via suppressing MT1-MMP.
In conclusion, our study reveals that down-regulation of KIF2A can inhibit gastric cancer cell invasion by suppressing MT1-MMP. This article is protected by copyright. All rights reserved. PMID: 29781531 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Pharmacology and Physiology - May 21, 2018 Category: Drugs & Pharmacology Authors: Zhao P, Lan F, Zhang H, Zeng G, Liu D Tags: Clin Exp Pharmacol Physiol Source Type: research

Delivery of small interfering RNA against Nogo-B receptor via tumor-acidity responsive nanoparticles for tumor vessel normalization and metastasis suppression.
This study demonstrated that NgBR is a promising therapeutic target in abnormal tumor vasculature and aggressive cancer cells, and the tumor-responsive nanoparticle with the feature of charge transformation offers great potential for tumor-specific delivery of gene therapeutics. PMID: 29803999 [PubMed - as supplied by publisher]
Source: Biomaterials - May 21, 2018 Category: Materials Science Authors: Wang B, Ding Y, Zhao X, Han X, Yang N, Zhang Y, Zhao Y, Zhao X, Taleb M, Miao QR, Nie G Tags: Biomaterials Source Type: research

The role and mechanism of KCa3.1 channels in human monocyte migration induced by palmitic acid.
Abstract Monocyte migration into diseased tissues contributes to the pathogenesis of diseases. Intermediate-conductance Ca2+-activated K+ (KCa3.1) channels play an important role in cell migration. However, the role of KCa3.1 channels in mediating monocyte migration induced by palmitic acid (PA) is still unclear. Using cultured THP-1 cells and peripheral blood mononuclear cells from healthy subjects, we investigated the role and signaling mechanisms of KCa3.1 channels in mediating the migration induced by PA. Using methods of Western blotting analysis, RNA interference, cell migration assay and ELISA, we found tha...
Source: Experimental Cell Research - May 21, 2018 Category: Cytology Authors: Ma XZ, Pang ZD, Wang JH, Song Z, Zhao LM, Du XJ, Deng XL Tags: Exp Cell Res Source Type: research

Sam68 Promotes Invasion, Migration, and Proliferation of Fibroblast-like Synoviocytes by Enhancing the NF- κB/P65 Pathway in Rheumatoid Arthritis
In this study, we analyzed the effect and possible mechanisms of Sam68 in rheumatoid arthritis (RA). By western blot analysis and immunohistochemistry, we found that the expression of Sam68 in synovial tissue of RA patients was increased compared with the cont rol group. Immunoflourescent staining demonstrated that Sam68 co-localized with fibroblast-like synoviocytes (FLS) of RA patients. Additionally, the expression of Sam68 in FLS was increased by tumor necrosis factor (TNF)-α stimulation, in a time-dependent manner. Upon TNF-α treatment, Sam68 transl ocated from the cytoplasm to the nucleus where it interacted with th...
Source: Inflammation - May 21, 2018 Category: Allergy & Immunology Source Type: research

Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.
Abstract The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties. Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect. Glycogen nanoparticles from different bio-sources and with different structural features were investigated. We show that larger gl...
Source: Biomaterials - May 20, 2018 Category: Materials Science Authors: Wojnilowicz M, Besford QA, Wu YL, Loh XJ, Braunger JA, Glab A, Cortez-Jugo C, Caruso F, Cavalieri F Tags: Biomaterials Source Type: research

AMPK regulates energy metabolism through the SIRT1 signaling pathway to improve myocardial hypertrophy.
CONCLUSIONS: The activation of AMPK can inhibit the myocardial hypertrophy, which may be realized through regulating the myocardial energy metabolism via SIRT1 signaling pathway. PMID: 29771428 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - May 19, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Long non-coding RNA DUXAP8 regulates proliferation and invasion of esophageal squamous cell cancer.
CONCLUSIONS: DUXAP8 expression was significantly correlated with tumor stage, lymph node metastasis and poor prognosis of ESCC patients. DUXAP8 may promote the occurrence of ESCC via Wnt/β-catenin pathway. PMID: 29771416 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - May 19, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Lipoxin A4 and its analog suppress hepatocarcinoma cell epithelial-mesenchymal transition, migration and metastasis via regulating integrin-linked kinase axis
Publication date: Available online 19 May 2018 Source:Prostaglandins & Other Lipid Mediators Author(s): Fen Xu, Xiaoyan Zhou, Jian Hao, Hua Dai, Jian Zhang, Yuanqiao He, Hua Hao Epithelial-mesenchymal Transition (EMT) and migration play an important role in tumor progression, and lipoxin (LX), the ‘stop signal’ for inflammation, has been studied in basic research for its anti-inflammatory or inflammatory pro-resolving properties. Here, in the in vitro experiment, we showed that LXA4 could inhibit the EMT and migration in phorbol myristate acetate (PMA) or activated conditioned medium (ACM)-stimulated Hep3B cel...
Source: Prostaglandins and Other Lipid Mediators - May 19, 2018 Category: Lipidology Source Type: research

Fibronectin Type III Domain Containing 4 attenuates hyperlipidemia-induced insulin resistance via suppression of inflammation and ER stress through HO-1 expression in adipocytes.
In conclusion, our results show that FNDC4 ameliorates insulin resistance via AMPK/HO-1-mediated suppression of inflammation and ER stress, indicating that FNDC4 may be a novel therapeutic agent for treating insulin resistance and type 2 diabetes. PMID: 29787756 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - May 19, 2018 Category: Biochemistry Authors: Lee W, Yun S, Choi GH, Jung TW Tags: Biochem Biophys Res Commun Source Type: research

Steroid Receptor Coactivator-Interacting Protein (SIP) Suppresses Myocardial Injury Caused by Acute Pancreatitis.
CONCLUSIONS In conclusion, the results suggested that SIP may inhibit the inflammatory response by deactivating p65, thus reducing the myocardial damage caused by AP. PMID: 29765014 [PubMed - in process]
Source: Medical Science Monitor - May 18, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Inhibition of fibroblast growth factor receptor-signaling sensitizes imatinib-resistant gastrointestinal stromal tumors to low doses of topoisomerase II inhibitors
The acquired resistance of gastrointestinal stromal tumors (GISTs) to the targeted-based therapy remains the driving force to identify the novel approaches that are capable of increasing the sensitivity of GISTs to the current therapeutic regimens. Our present data show that BGJ398, a selective fibroblast growth factor receptor (FGFR) inhibitor, sensitizes imatinib (IM)-resistant GIST cells with receptor tyrosine kinase (RTK) switch (loss of c-KIT/gain of pFGFR2a) to the low doses of topoisomerase II inhibitors – doxorubicin (Dox) and etoposide (Eto). Mechanistically, pretreatment of IM-resistant GIST cells with BGJ398 f...
Source: Anti-Cancer Drugs - May 18, 2018 Category: Cancer & Oncology Tags: Preclinical Reports Source Type: research

Munc13 ‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.
Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2. Mol Med Rep. 2018 May 14;: Authors: Xu R, Zhou J, Zhou XD, Li Q, Perelman JM, Kolosov VP Abstract The overexpression and hypersecretion of mucus is a hallmark of chronic pulmonary inflammatory disease. Mucin5AC (MUC5AC) is a major component of airway gel‑forming mucin. Members of the Unc13 (Munc13) protein family act as important activators of granule exocytosis from various types of mammalian cells. The present study aimed to determine the role of Munc13 family proteins in MUC5AC sec...
Source: Molecular Medicine Reports - May 18, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells
Publication date: 15 June 2018 Source:Life Sciences, Volume 203 Author(s): Zhiping Zeng, Yujing Wu, Yirong Cao, Ziying Yuan, Yuanqing Zhang, David Y. Zhang, Daisuke Hasegawa, Scott L. Friedman, Jinsheng Guo Background & aim Slit/Robo signaling was originally identified as a repulsive guidance cue in regulating axon branching and neuronal migration. Hepatic stellate cells (HSCs) are the key fibrogenic cells in the liver, which are migratory when activated, and express neural crest markers. The aim of the present study was to investigate the functional significance of Slit/Robo signaling in liver fibrogenesis and in...
Source: Life Sciences - May 18, 2018 Category: Biology Source Type: research

MEF2A regulates Calpain 3 expression in L6 myoblasts.
Abstract Calpain 3 (Capn3), a skeletal muscle-specific member of the calpain family, executes some non-proteolytic functions besides its role as a Ca2+-regulated proteolytic enzyme. Previously, we found that changes in Capn3 expression were linearly correlated with the degree of muscular atrophy following reversible sciatic nerve injury and that knockdown of Capn3 gene expression promoted myoblast differentiation. While the regulation of capn3 gene expression is interesting, transcriptional regulation of Capn3 is still unclear. In the present study, we provided experimental data showing that the myogenic enhancer ...
Source: Gene - May 18, 2018 Category: Genetics & Stem Cells Authors: Wu R, Wang J, Yao J, Dong Z, Liu Y, Liu M Tags: Gene Source Type: research

CDH17 alters MMP-2 expression via canonical NF- κB signalling in human gastric cancer.
CDH17 alters MMP-2 expression via canonical NF-κB signalling in human gastric cancer. Gene. 2018 May 18;: Authors: Jiang XJ, Lin J, Cai QH, Zhao JF, Zhang HJ Abstract Gastric cancer (GC), one of the most common cancers of the digestive system, results in high morbidity and mortality, but the molecular mechanisms underlying GC remain largely unknown. Cadherin-17 (CDH17) is a nonclassical member of the cadherin (CDH) superfamily of calcium-dependent proteins. Despite recent advances in the understanding of CDH17 biology, the mechanism of CDH17 in GC proliferation, migration, and invasion has not been e...
Source: Gene - May 18, 2018 Category: Genetics & Stem Cells Authors: Jiang XJ, Lin J, Cai QH, Zhao JF, Zhang HJ Tags: Gene Source Type: research

Acidic domain of WRNp is critical for autophagy and up-regulates age associated proteins.
Abstract Impaired autophagy may be associated with normal and pathological aging. Here we explore a link between autophagy and domain function of Werner protein (WRNp). Werner (WRN) mutant cell lines AG11395, AG05229 and normal aged fibroblast AG13129 display a deficient response to tunicamycin mediated endoplasmic reticulum (ER) stress induced autophagy compared to clinically unaffected GM00637 and normal young fibroblast GM03440. Cellular endoplasmic reticulum (ER) stress mediated autophagy in WS and normal aged cells is restored after transfection with wild type full length WRN, but deletion of the acidic domai...
Source: DNA Repair - May 18, 2018 Category: Genetics & Stem Cells Authors: Maity J, Das B, Bohr VA, Karmakar P Tags: DNA Repair (Amst) Source Type: research

A Paeonol derivative, YPH-PA3 promotes the differentiation of monocyte/macrophage lineage precursor cells into osteoblasts and enhances their autophagy.
Abstract Previous studies have indicated that paeonol inhibits RANKL-induced osteoclastogenesis by inhibiting the ERK, p38, and NF-κB pathway. We modified paeonol to form a new compound, YPH-PA3, and found that it promoted osteoclastogenesis rather than inhibited it the way paeonol does. The aim of this study is to investigate the mechanisms involved in YPH-PA3-promoted osteoclastogenesis. YPH-PA3-promoted differentiation of RAW264.7 cells (human monocytes) into osteoclasts is activated through ERK/p38/JNK phosphorylation, affecting c-FOS, NF-κB, and NFATc2. Real-time quantitative PCR and western blot revealed a...
Source: European Journal of Pharmacology - May 18, 2018 Category: Drugs & Pharmacology Authors: Tsai CH, Hsu MH, Huang PH, Hsieh CT, Chiu YM, Shieh DC, Lee YJ, Tsay GJ, Wu YY Tags: Eur J Pharmacol Source Type: research

MicroRNA ‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury.
MicroRNA‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury. Int J Mol Med. 2018 May 18;: Authors: Liang ZG, Yao H, Xie RS, Gong CL, Tian Y Abstract Myocardial ischemic injury results from severe impairment of the coronary blood supply and may lead to metabolic and ultrastructural changes, thereby causing irreversible damage. MicroRNA (miR)‑20b‑5p has been demonstrated to be involved in malignancies of the breast, colorectum, stomach, blood and oropharynx. The present study aimed to investigate the effects of ...
Source: International Journal of Molecular Medicine - May 18, 2018 Category: Molecular Biology Authors: Liang ZG, Yao H, Xie RS, Gong CL, Tian Y Tags: Int J Mol Med Source Type: research

MPC1 is essential for PGC-1{alpha}-induced mitochondrial respiration and biogenesis
In this study, we showed that MPC1 is down-regulated in human renal cell carcinoma (RCC) due to strong suppression of peroxisome proliferator-activated receptor-gamma co-activator (PGC)-1 alpha (PGC-1α). We also demonstrated that overexpression of PGC-1α stimulates MPC1 transcription, while depletion of PGC-1α by siRNA suppresses MPC expression. We found that PGC-1α interacts with estrogen-related receptor-alpha (ERR-α) and recruits it to the ERR-α response element motif located in the proximal MPC1 promoter, resulting in efficient activation of MPC1 expression. Furthermore, the MPC inhi...
Source: Biochemical Journal - May 18, 2018 Category: Biochemistry Authors: Koh, E., Kim, Y. K., Shin, D., Kim, K.-S. Tags: Research Articles Source Type: research

CXCR3 mediates chondrocyte injury through regulating nitric oxide.
CONCLUSIONS: Endoplasmic reticulum (ER) stress signaling pathway CHOP and GRP78 are involved in CXCR3 receptor attenuating chondrocyte apoptosis induced by SNP. PMID: 29762848 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - May 17, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

BST2 promotes cell proliferation, migration and induces NF- κB activation in gastric cancer.
CONCLUSION: BST2 possesses the oncogenic potential in GC by regulating the proliferation, apoptosis, and migratory ability of GC cells, thereby BST2 could be a potential therapeutic target for the treatment of GC. PMID: 29774441 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - May 17, 2018 Category: Biotechnology Authors: Liu W, Cao Y, Guan Y, Zheng C Tags: Biotechnol Lett Source Type: research

SM22 is required for the maintenance of actin-rich structures generated during bacterial infections.
Abstract The host actin cytoskeleton is utilized by an assortment of pathogenic bacteria to colonize and cause disease in their hosts. Two prominently studied actin-hijacking bacteria are enteropathogenic Escherichia coli (EPEC) and Listeria monocytogenes. EPEC form actin-rich pedestals atop its host cells to move across the intestinal epithelia, while Listeria monocytogenes generate branched actin networks arranged as actin clouds around the bacteria and as comet tails for propulsion within and amongst their host cells. Previous mass spectrometry analysis revealed that a member of the calponin family of actin-bun...
Source: Experimental Cell Research - May 17, 2018 Category: Cytology Authors: Chua MD, Hipolito KJ, Singerr OB, Solway J, Guttman JA Tags: Exp Cell Res Source Type: research

MicroRNA-137 dysregulation predisposes to osteoporotic fracture by impeding ALP activity and expression via suppression of leucine-rich repeat-containing G-protein-coupled receptor 4 expression.
In conclusion, these data prove that the overexpression of miR-137 was associated with an altered risk of fracture in patients with osteoporosis, and can be used as a biomarker for the prediction of risk of fracture in osteoporosis. PMID: 29786747 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - May 17, 2018 Category: Molecular Biology Authors: Liu X, Xu X Tags: Int J Mol Med Source Type: research

Pulmonary delivery of polyplexes for combined PAI-1 gene silencing and CXCR4 inhibition to treat lung fibrosis
This report describes the development of polyplexes based on CXCR4-inhibiting poly(ethylenimine) derivative (PEI-C) for pulmonary delivery of siRNA to silence plasminogen activator inhibitor-1 (siPAI-1) as a new combination treatment of pulmonary fibrosis (PF). Safety and delivery efficacy of the PEI-C/siPAI-1 polyplexes was investigated in vitro in primary lung fibroblasts isolated from mice with bleomycin-induced PF. Biodistribution analysis following intratracheal administration of fluorescently labeled polyplexes showed prolonged retention in the lungs.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - May 17, 2018 Category: Nanotechnology Authors: B.S. Ling Ding, B.S. Chenfei Zhu, M.S. Fei Yu, M.S. Pengkai Wu, M.S. Gang Chen, B.S. Aftab Ullah, Kaikai Wang, Minjie Sun, Jing Li, David Oupick ý Source Type: research

Design of PEI-conjugated bio-reducible polymer for efficient gene delivery
Publication date: 10 July 2018 Source:International Journal of Pharmaceutics, Volume 545, Issues 1–2 Author(s): Joung-Pyo Nam, Soyoung Kim, Sung Wan Kim The poly(cystaminebis(acrylamide)-diaminohexane) (poly(CBA-DAH)) was designed previously as a bio-reducible efficient gene delivery carrier. However, the high weight ratio required to form the polyplexes between poly(CBA-DAH) with pDNA is still a problem that needs to be addressed. To solve this problem and increase the transfection efficiency, poly(ethylenimine) (PEI, 1.8 kDa) was conjugated to poly(CBA-DAH) via disulfide bond. The PEI conjugated poly(CBA-DAH) (PCDP)...
Source: International Journal of Pharmaceutics - May 16, 2018 Category: Drugs & Pharmacology Source Type: research

G-protein Pathway Suppressor 2 (GPS2) enhanced the renal large conductance Ca2+ activated potassium channel expression via inhibiting ERK 1/2 signaling pathway.
In this study, we investigated the effects of GPS2 on BK channel activity and protein expression. In HEK BK stably-expressing cells transfected with either GPS2 or its pCMV vector control, a single cell recording showed that GPS2 significantly increased BK channel activity (NPo), increasing BK open probability (Po) and channel number (N) compared to the control. In Cos-7 cells and HEK293T cells, GPS2 overexpression significantly enhanced the total protein expression of BK in a dose-dependent manner. Knockdown of GPS2 expression significantly decreased BK protein expression while increasing ERK1/2 phosphorylation. Knockdown...
Source: Am J Physiol Renal P... - May 16, 2018 Category: Urology & Nephrology Authors: Zhuang Z, Xiao J, Chen X, Hu X, Li R, Chen S, Feng X, Shen S, Ma HP, Zhuang J, Cai H Tags: Am J Physiol Renal Physiol Source Type: research

Inhibition of Nogo-B promotes cardiac hypertrophy via endoplasmic reticulum stress
Publication date: August 2018 Source:Biomedicine & Pharmacotherapy, Volume 104 Author(s): Junli Li, Wenchao Wu, Yanguo Xin, Mingyue Zhao, Xiaojing Liu Aims Nogo-B is a key endoplasmic reticulum (ER) protein that regulates ER stress signaling. However, its role in cardiac hypertrophy remains poorly understood. ER stress is interrelated with autophagy in the process of cardiac hypertrophy. Therefore, we aimed to test the hypothesis that both ER stress and autophagy signaling mediate the function of Nogo-B in cardiac hypertrophy. Main methods Rat models of transverse aortic constriction (TAC), neonatal rat cardiomyoc...
Source: Biomedicine and Pharmacotherapy - May 16, 2018 Category: Drugs & Pharmacology Source Type: research

Pulmonary delivery of polyplexes for combined PAI-1 gene silencing and CXCR4 inhibition to treat lung fibrosis
This report describes the development of polyplexes based on CXCR4-inhibiting poly(ethylenimine) derivative (PEI-C) for pulmonary delivery of siRNA to silence plasminogen activator inhibitor-1 (siPAI-1) as a new combination treatment of pulmonary fibrosis (PF). Safety and delivery efficacy of the PEI-C/siPAI-1 polyplexes was investigated in vitro in primary lung fibroblasts isolated from mice with bleomycin-induced PF. Biodistribution analysis following intratracheal administration of fluorescently labeled polyplexes showed prolonged retention in the lungs. Treatment of mice with bleomycin-induced PF using the PEI-C/siPAI-...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 16, 2018 Category: Nanotechnology Source Type: research

Amelioration of myocardial ischemia-reperfusion injury by SIRT4 involves mitochondrial protection and reduced apoptosis.
Abstract Apoptosis and mitochondria dysfunction are key contributors to myocardial ischemia-reperfusion (MI-R) injury. SIRT4, a mitochondrial-localized sirtuin, controls cellular energy metabolism and stress response, and is abundantly present in the heart, however, its role in MI-R injury is not clear. In the current study, we demonstrate that SIRT4 is downregulated in cardiomyocytes both in vitro and in vivo models after MI-R. Functionally, SIRT4 overexpression decreases myocardial infarct size and serum creatine phosphokinase (CPK) level, and vice versa, SIRT4 depletion by siRNA increases myocardial infarct siz...
Source: Biochemical and Biophysical Research communications - May 16, 2018 Category: Biochemistry Authors: Zeng G, Liu H, Wang H Tags: Biochem Biophys Res Commun Source Type: research

Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1 ‑tP proteins.
Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. Int J Mol Med. 2018 May 16;: Authors: Zeng Y, Li Z, Shang J, Kang Y Abstract Hepatitis B virus (HBV) infection and related liver complications remain severe public health problems worldwide. Previous investigations have shown that small interfering (si)RNAs can offer an effective strategy for the treatment of chronic hepatitis B. The present study aimed to develop a novel siRNA‑delivering system of therapeutic HBV nuclear localization sequence (NLS) siRNAs using the recombinan...
Source: International Journal of Molecular Medicine - May 16, 2018 Category: Molecular Biology Authors: Zeng Y, Li Z, Shang J, Kang Y Tags: Int J Mol Med Source Type: research

Asymmetric dimethylarginine contributes to retinal neovascularization of diabetic retinopathy through EphrinB2 pathway.
In conclusion, ADMA contributes to the neovascularization of retina in diabetic mellitus, which is regulated by ephrinB2. PMID: 29777874 [PubMed - as supplied by publisher]
Source: Vascular Pharmacology - May 16, 2018 Category: Drugs & Pharmacology Authors: Du MR, Yan L, Li NS, Wang YJ, Zhou T, Jiang JL Tags: Vascul Pharmacol Source Type: research

COP9 signalosome subunit 5 regulates cancer metastasis by deubiquitinating SNAIL.
In this study, we identified COP9 signalosome subunit 5 (COPS5) as a deubiquitinating enzyme of SNAIL by using siRNA library screening. COPS5 downregulation significantly reduced the expression of SNAIL and impaired the metastatic potential of lung cancer cells both in vitro and in vivo. Importantly, we demonstrated that COPS5 binds to SNAIL and stabilizes its expression by deubiquitination. Furthermore, we observed the positive correlation between COPS5 and SNAIL expression in the clinical tissue samples of lung adenocarcinomas by using tissue microarray analysis. These findings provide strong evidence that COPS5 can be a...
Source: Oncotarget - May 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research