Query: siRNA

This page shows you your search results in order of date. This is page number 12.

Order by Relevance | Date

Total 14406 results found since Jan 2013.

Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation
Conclusion: Our results demonstrate for the first time that AGP induces RASSF1A expression in colon cancer cells and is dependent on angiogenesis signaling events. Therefore, our research may facilitate novel therapeutic options for advanced colon cancer therapy.Cell Physiol Biochem 2018;48:1259 –1273
Source: Cellular Physiology and Biochemistry - July 25, 2018 Category: Cytology Source Type: research

Long non-coding RNA TRPM2-AS as a potential biomarker for hepatocellular carcinoma
ConclusionLong non-coding RNA TRPM2-AS is upregulated in HCC and represents a new biomarker for HCC and the inhibition of TRPM2-AS promotes apoptosis in HCC cells in vitro.
Source: Irish Journal of Medical Science - July 25, 2018 Category: General Medicine Source Type: research

Engineering of budesonide-loaded lipid-polymer hybrid nanoparticles using a quality-by-design approach
This study shows the importance of systematic formulation design for understanding the effect of formulation parameters on the characteristics of LPNs, eventually resulting in the identification of an OOS.Graphical abstract
Source: International Journal of Pharmaceutics - July 25, 2018 Category: Drugs & Pharmacology Source Type: research

GSE113923 RNA-dependent epigenetic silencing directs transcriptional down regulation caused by intronic repeat expansions
Contributors : Hannes Eimer ; Sridevi Sureshkumar ; Avilash S Yadav ; Calvin Kraupner-Taylor ; Champa Bandaranayake ; Andrei Seleznev ; Tamblyn Thomason ; Stephen Fletcher ; Stephanie F Gordon ; Bernard J Carroll ; Sureshkumar BalasubramanianSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaTranscriptional down regulation caused by intronic triplet repeat expansions underlies diseases such as Friedreich?s ataxia. This down regulation of gene expression is coupled with epigenetic changes but the underlying mechanisms are unknown. Here, we show that an intronic TTC/GAA triplet exp...
Source: GEO: Gene Expression Omnibus - July 25, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

Targeting autophagy by small molecule inhibitors of vacuolar protein sorting 34 (Vps34) improves the sensitivity of breast cancer cells to Sunitinib
In this study we systematically screened a library of 306 known anti-cancer drugs for their ability to induce autophagy using a cell-based assay. 114 of the drugs were classified as autophagy inducers; for 16 drugs, the cytotoxicity was potentiated by siRNA-mediated knock-down of Atg7 and Vps34. These drugs were further evaluated in breast cancer cell lines for autophagy induction, and two tyrosine kinase inhibitors, Sunitinib and Erlotinib, were selected for further studies.
Source: Cancer Letters - July 25, 2018 Category: Cancer & Oncology Authors: Matheus Dyczynski, Yasmin Yu, Magdalena Otrocka, Santiago Parpal, Tiago Braga, Aine Brigette Henley, Henric Zazzi, Mikael Lerner, Krister Wennerberg, Jenny Viklund, Jessica Martinsson, Dan Grand ér, Angelo De Milito, Katja Pokrovskaja Tamm Tags: Original Articles Source Type: research

Glucosamine abrogates the stem cell factor  + endothelin-1-induced stimulation of melanogenesis via a deficiency in MITF expression due to the proteolytic degradation of CREB in human melanocytes
In this study, we characterized the molecular mechanism involved in the anti-melanogenic effects of GlcN using normal human melanocytes (NHMs) in culture. The SE-stimulated gene (12 h) and protein (24 h) expression levels of melanocyte-specific proteins (at the indicated times post-stimulation) were significantly abrogated by pretreatment with GlcN for 72 h. Western blotting analysis of the phosphorylation of intracellular signaling molecules in the MAPK pathway revealed that despite the significantly decreased level of total CREB protein at all times post-stimulat ion, the SE-stimulated phosphorylation of ERK, CREB and...
Source: Archives of Dermatological Research - July 25, 2018 Category: Dermatology Source Type: research

O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death
In this study, co-incubation of HepG2 cells with E. histolytica increased DNA fragmentation and LDH release as compared with control cells. Additionally, Gal-lectin-mediated amoebic adherence of live trophozoites to HepG2 cells decreased O-GlcNAcylated protein levels within 5 min. We also observed a rapid decrease in cellular OGT protein level, but not OGA, in HepG2 cells in a contact-dependent manner. Furthermore, HepG2 pretreatment with OGA inhibitors or OGA siRNA prevented E. histolytica-induced O-deGlcNAcylation, DNA fragmentation, and LDH release. Our results suggested that E. histolytica-induced O-deGlcNAcylation i...
Source: Microbial Pathogenesis - July 24, 2018 Category: Infectious Diseases Source Type: research

Expression of BANCR promotes papillary thyroid cancer by targeting thyroid stimulating hormone receptor.
Authors: Zheng H, Xu J, Hao S, Liu X, Ning J, Song X, Jiang L, Liu Z Abstract Papillary thyroid carcinoma (PTC) is the most common form of non-medullary thyroid cancer, accounting for ~80% of all cases of thyroid cancer. The aim of the present study was to explore the role of BRAF-activated long noncoding RNA (BANCR) in the development of PTC. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the mRNA expression levels of BANCR, thyroid-stimulating hormone receptor (TSHR) and cyclin D1 between PTC and benign control thyroid nodule tissue samples from 60 patients were determined. Using RT...
Source: Oncology Letters - July 24, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Roles of the MST1-JNK signaling pathway in apoptosis of colorectal cancer cells induced by Taurine.
Authors: Liu Z, Xia Y, Zhang X, Liu L, Tu S, Zhu W, Yu L, Wan H, Yu B, Wan F Abstract The aim of this study was to observe the impact of the mammalian sterile 20-like kinase 1-c-Jun N-terminal kinase (MST1-JNK) signaling pathway on apoptosis in colorectal cancer (CRC) cells induced by Taurine (Tau). Caco-2 and SW620 cells transfected with p-enhanced green fluorescent protein (EGFP)-MST1 or short interfering RNA (siRNA)-MST1 were treated with Tau for 48 h. Apoptosis was detected by flow cytometry, and the levels of MST1 and JNK were detected by western blotting. Compared with the control group, 80 mM Tau could sig...
Source: Libyan Journal of Medicine - July 24, 2018 Category: General Medicine Tags: Libyan J Med Source Type: research

The prostaglandin EP4 receptor is a master regulator of the expression of PGE2 receptors following inflammatory activation in human monocytic cells
Publication date: Available online 24 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Alaa Kashmiry, Rothwelle Tate, Giuliana Rotondo, Jillian Davidson, Dino RotondoAbstractProstaglandin E2 (PGE2) is responsible for inflammatory symptoms. However, PGE2 also suppresses pro-inflammatory cytokine production. There are at least 4 subtypes of PGE2 receptors, EP1–EP4, but it is unclear which of these specifically control cytokine production. The aim of this study was to determine which of the different receptors, EP1R–EP4R modulate production of tumor necrosis factor-α (...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 24, 2018 Category: Lipidology Source Type: research

PEGylated DC-Chol/DOPE cationic liposomes containing KSP siRNA as a systemic siRNA delivery Carrier for ovarian cancer therapy.
Abstract Although siRNA-mediated downregulation technology has been highly successful in suppressing the expression of any disease-related gene, systemic delivery of siRNA for the clinical applications remains challenging, especially in the use of cancer therapy. DC-Chol/DOPE cationic liposomes as one of the most attractive vehicles for gene delivery have been widely exploited for transfection of siRNA into cells, but complexity of systemic delivery has allowed only their direct injection into local targets due to the formation of aggregations with negatively-charged blood components. Herein, we demonstrate the ef...
Source: Biochemical and Biophysical Research communications - July 23, 2018 Category: Biochemistry Authors: Lee J, Ahn HJ Tags: Biochem Biophys Res Commun Source Type: research

Influence of controlled release of resveratrol from electrospun fibers in combination with siRNA on leukemia cells.
In this study, we evaluated the possibility of i) local release of resveratrol from poly(ε-caprolactone) (PCL) and gelatin (GT) electrospun fibers and ii) combining (i) with siRNA designed to downregulate BCR-ABL pathway on K562 cancer cells. Initially, K562 cell culture experiments were performed using various bolus doses of resveratrol in combination with siRNA for 3 days using a factorial design of experiments approach. Resveratrol content was analyzed using HPLC and cell viability was assessed using Annexin V (Non-viable), and Propidium Iodide (PI) (Necrotic) based flow cytometry. Coaxial electrospun fibers with res...
Source: European Journal of Pharmaceutical Sciences - July 21, 2018 Category: Drugs & Pharmacology Authors: Al-Attar T, Madihally SV Tags: Eur J Pharm Sci Source Type: research

SiRNA interfering STAT3 enhances DDP sensitivity in cervical cancer cells.
CONCLUSIONS: STAT3 over-expression is associated with DDP resistance in cervical cancer. Decreasing STAT3 can significantly promote the apoptosis of cervical cancer CaSki cells and decrease the DDP resistance. PMID: 30024597 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - July 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

siRNA Targeting of the < b > < i > SNCG < /i > < /b > Gene Inhibits the Growth of Gastric Carcinoma SGC7901 Cells in vitro and in vivo < b > < /b > by Downregulating the Phosphorylation of AKT/ERK
The aim of the study was to evaluate the effects of synuclein-#x03B3; (SNCG) silencing on gastric cancer SGC7901 cells and to elucidate the associated mechanisms. pGCSIL-lentiviral siRNA targeting of theSNCG gene was employed to inhibitSNCG expression. Several experiments such as quantitative real-time PCR, Western blotting, MTT, colony formation, migration assay, and flow cytometry were performed to investigate the biological behavior of infected SGC7901 cells. BALB/c nude mice were used as tumor xenograft models to assess the effects ofSNCG silencing on tumor growth. Western blot analysis was carried out to determine the...
Source: Cytogenetic and Genome Research - July 20, 2018 Category: Genetics & Stem Cells Source Type: research

Diethylaminoethyl- chitosan as an efficient carrier for siRNA delivery: Improving the condensation process and the nanoparticles properties.
Abstract Chitosan has been indicated as a promising carrier for the preparation of small interfering RNA (siRNA) delivery systems due to its remarkable properties. However, its weak interactions with siRNA molecules makes the condensation of siRNA molecules into nanoparticles difficult. In this work, a non-viral gene delivery system based on diethylaminoethyl chitosan (DEAE-CH) derivatives of varied Mw (25-230 kDa) having a low degree of substitution of 15% was investigated. The presence of secondary and tertiary amino groups strengthened the interaction of siRNA and DEAE-CH derivatives of higher Mw (130 kDa t...
Source: International Journal of Biological Macromolecules - July 18, 2018 Category: Biochemistry Authors: de Souza RHFV, Picola IPD, Shi Q, Petrônio MS, Benderdour M, Fernandes JC, Lima AMF, Martins GO, Junior AMM, de Oliveira Tiera VA, Tiera MJ Tags: Int J Biol Macromol Source Type: research

Micelle-like nanoparticles as siRNA and miRNA carriers for cancer therapy.
Abstract Gene therapy has emerged as an alternative in the treatment of cancer, particularly in cases of resistance to chemo and radiotherapy. Different approaches to deliver genetic material to tumor tissues have been proposed, including the use of small non-coding RNAs due to their multiple mechanisms of action. However, such promise has shown limits in in vivo application related to RNA's biological instability and stimulation of immunity, urging the development of systems able to overcome those barriers. In this review, we discuss the use of RNA interference in cancer therapy with special attention to the role...
Source: Biomedical Microdevices - July 12, 2018 Category: Biomedical Engineering Authors: Costa DF, Torchilin VP Tags: Biomed Microdevices Source Type: research

Nanoscale polyelectrolyte complexes encapsulating mRNA and long-chained siRNA for combinatorial cancer gene therapy
Publication date: 25 August 2018Source: Journal of Industrial and Engineering Chemistry, Volume 64Author(s): Myung Goo Kim, Sung Duk Jo, Ji Hoon Jeong, Sun Hwa KimAbstractTo precisely regulate target genes that are abnormally expressed in cancers, we suggest an RNA-mediated multigene targeting system that co-encapsulates siRNA against vascular endothelial growth factor (VEGF) and mRNA encoding phosphatase and tensin homolog (PTEN). Polymerized long-chain siRNAs (L-siRNAs) formed stable and condensed nanocomplexes with mRNAs using thiolated glycol chitosans (tGCs) as gene carriers. The mRNA/L-siRNA/tGC nanocomplexes (MSNs) ...
Source: Journal of Industrial and Engineering Chemistry - July 11, 2018 Category: Chemistry Source Type: research

Cellular uptake mechanism and comparative in vitro cytotoxicity studies of monomeric LMWP-siRNA conjugate
Publication date: 25 July 2018Source: Journal of Industrial and Engineering Chemistry, Volume 63Author(s): Junxiao Ye, Xing Pei, Hui Cui, Zhili Yu, Hyukjin Lee, Jianxin Wang, Xu Wang, Lu Sun, Huining He, Victor C. YangAbstractThe covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical tr...
Source: Journal of Industrial and Engineering Chemistry - July 11, 2018 Category: Chemistry Source Type: research

Potential of siRNA-albumin complex against cancer
Publication date: Available online 27 April 2018Source: Chemico-Biological InteractionsAuthor(s): Na Liu, Yan-Hua Qi, Chuan-Tao Cheng, Wen Bin Yang, Anshoo Malhotra, Qi ZhouAbstractRNA interference is a highly specific as well as efficient technology for gene therapy application in molecular oncology. The present study was planned to develop an efficient and stable tumor selective delivery mechanism for siRNA gene therapy for the purpose of both diagnosis as well as therapy. We have used 20 Male wistar rats for the formation of colon cancer model and utilized albumin as carrier molecule for the delivery of siRNA against va...
Source: Chemico Biological Interactions - July 11, 2018 Category: Biochemistry Source Type: research

Evaluation of siRNA and Cationic Liposomes Complexes as a Model for in vitro siRNA Delivery to Cancer Cells
This study aims to investigate the effect of different molar charge ratios (R+/-) between positive charges from microfluidics-produced cationic liposomes (CL) (egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and negative charges from siRNA and on physico-chemical and morphological properties of the lipoplexes (CL/siRNA) as well as their in vitro luciferase silencing effect in HeLa cells. R+/- 3.27 was found to be the optimum point for complexation. This finding was confirmed by gel retardation and siRNA accessibility assays. According to Cryo-TEM analysis...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - July 11, 2018 Category: Chemistry Source Type: research

AMF siRNA treatment of keloid through inhibition signaling pathway of RhoA/ROCK1
In conclusion, AMF siRNA is expected to be a novel strategy to treat KD by inhibiting signaling pathway of RhoA/ROCK1.
Source: Genes and Diseases - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies
Conclusions and general significanceThese basic associations are equivalent to those observed in D. melanogaster, implying that they have been evolutionary conserved from at least cockroach to flies, and possibly stem from the last common ancestor of extant insects.
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

STAT3-siRNA induced B16.F10 melanoma cell death: more association with VEGF downregulation than p-STAT3 knockdown
Publication date: Available online 30 May 2018Source: Saudi Pharmaceutical JournalAuthor(s): Aws AlshamsanAbstractSTAT3 knockdown by small interfering RNA (siRNA) has been described to inhibit carcinogenic growth in various types of tumors. Earlier we have reported delivery of siRNA by oleic acid- and stearic acid-modified-polyethylenimine and enhancement of silencing of STAT3 by small interfering RNA (siRNA) in B16.F10 melanoma cell lines and consequent tumor suppression. Present investigation mainly focused on the downstream events involved in B16.F10 melanoma cell death and consequent tumor suppression following knockdo...
Source: Saudi Pharmaceutical Journal - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery
Publication date: Available online 21 February 2018Source: Asian Journal of Pharmaceutical SciencesAuthor(s): Anna Slita, Anna Egorova, Eudald Casals, Anton Kiselev, Jessica M. RosenholmAbstractGene therapy using siRNA molecules is nowadays considered as a promising approach. For successful therapy, development of a stable and reliable vector for siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles (MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid carrying capacity. To overcome this hurdle, we here modif...
Source: Asian Journal of Pharmaceutical Sciences - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Enhancing the therapeutic effect via elimination of hepatocellular carcinoma stem cells using Bmi1 siRNA delivered by cationic cisplatin nanocapsules
Publication date: Available online 26 May 2018Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Tan Yang, Yuyuan Chen, Pengxuan Zhao, Huiying Xue, Jia You, Bin Li, Yong Liu, Chuanchuan He, Xiaojuan Zhang, Lingling Fan, Robert J. Lee, Lei Li, Xiang Ma, Chuanrui Xu, Guangya XiangResistance of hepatocellular carcinoma (HCC) to systemic chemotherapy is partially due to presence of drug-resistant cancer stem cells. Bmi1 protein is essential for survival and proliferation of HCC cancer stem cells (CSCs). Here, we report that Bmi1 siRNA (Bmi1siR) loaded in cationic nanocapsules of cisplatin (NPC) eliminated ste...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 10, 2018 Category: Nanotechnology Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Publication date: Available online 22 June 2017Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping XuAbstractSheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moie...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 10, 2018 Category: Nanotechnology Source Type: research

Pre-Injection of Small Interfering RNA (siRNA) Promotes c-Jun Gene Silencing and Decreases the Survival Rate of Axotomy-Injured Spinal Motoneurons in Adult Mice
AbstractBrachial plexus injury is a common clinical peripheral nerve trauma. A series of genes in motoneurons were activated in the corresponding segments of the spinal cord after brachial plexus roots axotomy. The spatial and temporal expression of these genes directly affects the speed of motoneuron axon regeneration and precise target organ reinnervation. In a previous study, we observed the overexpression of c-Jun in motoneurons of the spinal cord ventral horn after brachial plexus injury in rats. However, the relevance of c-Jun expression with respect to the fate of axotomy-induced branchial plexus injury in adult mic...
Source: Journal of Molecular Neuroscience - July 10, 2018 Category: Neuroscience Source Type: research

Nanovehicle-based small interfering RNA (siRNA) delivery for therapeutic purposes: a new molecular approach in the pharmacogenomics.
Abstract RNA interference (RNAi) is a process for regulating the gene expression in which small interfering RNAs (siRNAs) silence target genes. siRNA-based therapy as a new molecular treatment approach, offers therapeutic prospects for many common diseases such as cancer and cardiovascular disorders. Nevertheless, the efficacy of siRNA delivery has, so far, remained a challenging issue. This is due to their easy degradation through the circulation system and the difficulties in the intracellular delivery to specific tissues where they silence the target genes. There have been many efforts to develop suitable, safe...
Source: Current Clinical Pharmacology - July 9, 2018 Category: Drugs & Pharmacology Authors: Akhtari J, Tafazoli A, Mehrad-Majd H, Mahrooz A Tags: Curr Clin Pharmacol Source Type: research

Evaluation of siRNA and Cationic Liposomes Complexes as a Model for in vitro siRNA Delivery to Cancer Cells
This study aims to investigate the effect of different molar charge ratios (R+/-) between positive charges from microfluidics-produced cationic liposomes (CL) (egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and negative charges from siRNA and on physico-chemical and morphological properties of the lipoplexes (CL/siRNA) as well as their in vitro luciferase silencing effect in HeLa cells. R+/- 3.27 was found to be the optimum point for complexation. This finding was confirmed by gel retardation and siRNA accessibility assays. According to Cryo-TEM analysis...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - July 6, 2018 Category: Chemistry Source Type: research

Potential of siRNA-albumin complex against cancer
Publication date: Available online 27 April 2018Source: Chemico-Biological InteractionsAuthor(s): Na Liu, Yan-Hua Qi, Chuan-Tao Cheng, Wen Bin Yang, Anshoo Malhotra, Qi ZhouAbstractRNA interference is a highly specific as well as efficient technology for gene therapy application in molecular oncology. The present study was planned to develop an efficient and stable tumor selective delivery mechanism for siRNA gene therapy for the purpose of both diagnosis as well as therapy. We have used 20 Male wistar rats for the formation of colon cancer model and utilized albumin as carrier molecule for the delivery of siRNA against va...
Source: Chemico Biological Interactions - July 5, 2018 Category: Biochemistry Source Type: research

i-Fect Delivers siRNA to Study Angiogenesis in Brain Injury
This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.A mixture of ouri-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita.Extracellular Lactate Dehydrogenase A Release ...
Source: siRNA and DsiRNA Transfection Efficiency - February 19, 2018 Category: Neuroscience Tags: Angiogenesis Delivering siRNA to the CNS i-Fect Lactate Dehydrogenase A LDHA siRNA delivery to mouse brain Source Type: news

Delivering siRNA in vivo
New Publications Ouri-Fect transfection kit was one of the first products. This has resulted in it being widely used andfrequently published.We kick off 2018 with some new publications. Here we feature a study where researchers use our kit to deliver G-protein-coupled receptor C (MrgprC) siRNA in vivo. This receptor is part of the TRPV1 pathway.Ying-jun Liu, Xiao-xi Lin, Jian-qiao Fang, and Fang Fang. Involvement of MrgprC in electroacupuncture analgesia for attenuating CFA-induced thermal hyperalgesia by suppressing the TRPV1 pathway. Pre-pub-full text PDFImage: Expression of TRPV1 in rats treated with MrgrprC s...
Source: siRNA and DsiRNA Transfection Efficiency - January 13, 2018 Category: Neuroscience Tags: inflammatory pain intrathecal delivery of siRNA Mas-related G-protein-coupled receptor C MrgrpfC TRPV1 Source Type: news

Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: siRNA and DsiRNA Transfection Efficiency - March 24, 2016 Category: Neuroscience Tags: epigenetics Ga9 Mu Opioid Receptor inhibitor i-Fect iFect intrathecal delivery of siRNA Morphine neuropathic pain Source Type: news

Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed
Potent and Frequently Published Transfection Solutions We have proven and frequently published transfection solutions. Powerful punch and versatility are now needed more than ever with the hyper growth in gene manipulation technologies like Sleeping BeautyTM and CRISPR-Cas9.We have published examples of the use of our solutions for delivering siRNA, miRNA, shRNA and plasmids both in vitro and in vivo. Here are some of your options. i-Fect ™ -A novel cationic lipid formulation specifically designed for efficient delivery of 27mer DsiRNAs(dicer substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNA...
Source: siRNA and DsiRNA Transfection Efficiency - January 20, 2016 Category: Neuroscience Tags: DNA transfection i-Fect miRNA Delivery Methods p-fect Plasmid Transfection plasmids pn-Fect shRNA siRNA Source Type: news

Silencing Cytokines in-vivo with i-Fect
Knocking Down Cytokine to Study Pain ResponseWe have many unique applications published from researchers using our Transfection Kits in vitro and in vivo. Here researchers simultaneously 3 immune/inflammatory response cytokines in vivo: Byung Moon Choi, Soo Han Lee, Sang Mee An, Do Yang Park, Gwan Woo Lee, and Gyu-Jeong Noh. The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats. Korean J Anesthesiol. 2015 Apr;68(2):159-169. English. Published online March 30, 2015. http://dx.doi.org/10.4097/kjae.2015.68.2.159.Protocol: RNAs w...
Source: siRNA and DsiRNA Transfection Efficiency - April 7, 2015 Category: Neuroscience Tags: allodynia analgesia Gene Expression Analysis Gene Silencing i-Fect IL-1B IL-6 inflammatory pain intrathecal delivery of siRNA TNF-alpha Transfection Kits Source Type: news

i-Fect Delivers siRNA in vitro and in vivo
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: Neuromics - February 21, 2015 Category: Neuroscience Tags: Cerebral Ischemia Connexin 43 Delivering siRNA to the CNS EPO HDAC2 i-Fect Stroke Source Type: news

EPO Protects Against Cerebral Ischemia
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: siRNA and DsiRNA Transfection Efficiency - February 18, 2015 Category: Neuroscience Tags: cerebral ischemia Connexin 43 Delivering siRNA to the CNS i-Fect iFect siRNA transfection of neurons Source Type: news

Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors  for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Gene Expression Analysis For Neuroscientists
In Vitro and In Vivo StudiesNeuromics has a strong line up of Transfection Tools designed specifically for Neuroscientists. Neurons, Glia and Astrocytes are notoriously hard to transfect. We are proud of our track record.This is an excellent study for Neuroscience Researchers interested in using best methods: http://emboj.embopress.org/content/embojnl/31/15/3239/DC1/embed/inline-supplementary-material-1.pdf?download=true.  Here researchers delivered 14-3-3 siRNA sub units + i-Fect intrathecally. Here's specific knockdown results:Figure: A. Dose-dependent inhibition of 14-3-3-zeta (z) expression with ant...
Source: siRNA and DsiRNA Transfection Efficiency - June 25, 2014 Category: Neuroscience Tags: 14-3-3 zeta i-Fect in vivo RNAi intrathecal delivery of siRNA intravenous delivery of siRNA siRNA in vivo Transfection Source Type: news

Hybrid lipid–polymer nanoparticles for sustained siRNA delivery and gene silencing
Abstract: The development of controlled-release nanoparticle (NP) technologies has great potential to further improve the therapeutic efficacy of RNA interference (RNAi), by prolonging the release of small interfering RNA (siRNA) for sustained, long-term gene silencing. Herein, we present an NP platform with sustained siRNA-release properties, which can be self-assembled using biodegradable and biocompatible polymers and lipids. The hybrid lipid–polymer NPs showed excellent silencing efficacy, and the temporal release of siRNA from the NPs continued for over one month. When tested on luciferase-expressed HeLa cells and A...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - March 19, 2014 Category: Nanotechnology Authors: Jinjun Shi, Yingjie Xu, Xiaoyang Xu, Xi Zhu, Eric Pridgen, Jun Wu, Alexander R. Votruba, Archana Swami, Bruce R. Zetter, Omid C. Farokhzad Tags: Pharmacology, Nano-Enabled Drug Delivery, Controlled Release, Polymer-siRNA Hybrid Source Type: research

i-Fect, BDNF and Irritable Bowel Syndrome (IBS)
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: siRNA and DsiRNA Transfection Efficiency - March 11, 2014 Category: Neuroscience Tags: BDNF;chronic stress;functional bowel disorders;iritable bowel syndrome;visceral hypersnsitivity i-Fect intrathecal delivery of siRNA Source Type: news

Preclinical development of siRNA therapeutics: Towards the match between fundamental science and engineered systems
Abstract: The evolution of synthetic RNAi faces the paradox of interfering with the human biological environment. Due to the fact that all cell physiological processes can be target candidates, silencing a precise biological pathway could be challenging if target selectivity is not properly addressed. Molecular biology has provided scientific tools to suppress some of the most critical issues in gene therapy, while setting the standards for siRNA clinical application. However, the protein down-regulation through the mRNA silencing is intimately related to the sequence-specific siRNA ability to interact accurately with the ...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - December 11, 2013 Category: Nanotechnology Authors: M. Videira, A. Arranja, D. Rafael, R. Gaspar Tags: Cell Biology and Genetics, Amino Acids, Nucleic Acids, siRNA Therapy, Basic Science vs. Engineering Source Type: research

Using i-Fect to Knock Down GLT-1 Gene in vivo
Pain Researchers continue to use our i-FectTM Transfection Kit for Gene Expression AnalysisValproate Prevents Dysregulation of Spinal Glutamate and Reduces the Development of Hypersensitivity in Rats After Peripheral Nerve Injury. The Journal of Pain, Volume 14, Issue 11, November 2013, Pages 1485–1491 http://dx.doi.org/10.1016/j.jpain.2013.07.007.Researchers use the kit to deliver Glutamate Receptor 1 siRNA in vivo.The present study examined whether the histone deacetylase inhibitor valproate prevents downregulation of glutamate transporters in the primary cultured astrocytes and in the spinal cord after L5-L6 spinal ne...
Source: siRNA and DsiRNA Transfection Efficiency - November 6, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS GLT-1 Gene Glutamate Receptor 1 siRNA in vivo i-Fect intrathecal delivery of siRNA Source Type: news

i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of  Neuromics'  Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news

i-Fect Delivers IGF1 siRNA to the Mouse Brain
Intraventricular Injections Used to Study Neuronal Survival During Post Natal DevelopmentNeuromics' i-FectTM Transfection Kit continue to be successfully used to deliver siRNA, shRNA and miRNA to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection). Genes studied include: ABCA, ASIC, β-arrestin, CAV1.2, IGF1, CX3CR1, DOR, ELOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 ,The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK,SNSR1, hTertTRPV1 NOV, Survivin, TLR4, Troy and TRPV1. Related Publications.It is always an honor for one our products to be referenced in one of the...
Source: siRNA and DsiRNA Transfection Efficiency - April 25, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo siRNA delivery to mouse brain i-Fect Gene Silencing intraventicular injections IGF1 gene expression in vivo siRNA Source Type: news

i-Fect™ Delivers Your siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: Neuromics - March 16, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo Delivering siRNA to the CNS KV receptors. i-Fect Kv9.1 Potassium Channel Source Type: news

i-Fect™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Kv9.1 Potassium Channel Subunit kv1.1 Potassium Channel Subunit Delivering siRNA to the CNS Transfection Kits KV Receptors i-Fect Dorsal root ganglia intrathecal delivery of siRNA in vivo siRNA Source Type: news

i-Fect ™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence K V Receptors. Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons . In these 2 latest expamples, researchers use i-Fect to deliver siRNA to K V Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570 –579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS Dorsal root ganglia i-Fect in vivo siRNA intrathecal delivery of siRNA KV Receptors kv1.1 Potassium Channel Subunit Kv9.1 Potassium Channel Subunit Transfection Kits Source Type: news

ABCA siRNA Transfection of Neurons & Astrocytes
In this study, Dr. Marina Guizzetti and his team use our i-Fect Kit and several other commercially available kits to knockdown ABCG1 and ABCG4: Jing Chen, Xiaolu Zhang, Handojo Kusumo, Lucio G. Costa, c, Marina Guizzettia. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 (2), p.263-275, Feb 2013. doi:10.1016/j.bbalip.2012.09.007. Abstract: Disruption of cholesterol homeostasis in the central nervous system (CNS) has been associated with neurological, neurodegenerative...
Source: siRNA and DsiRNA Transfection Efficiency - November 17, 2012 Category: Neuroscience Tags: ABCA1 i-Fect siRNA Delivery in vitro siRNA Transfection Efficiency siRNA transfection of neurons siRNA trasfection of astrocytes Source Type: news