Query: siRNA

This page shows you your search results in order of date. This is page number 12.

Order by Relevance | Date

Total 16013 results found since Jan 2013.

The JAK2/STAT3 signaling pathway is required for inflammation and cell death induced by cerulein in AR42J cells.
CONCLUSIONS: These findings point to a novel role for the JAK2/STAT3 signaling pathway in the progression of cerulein-induced AP. PMID: 30840302 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Autophagy in pulmonary macrophages mediates lung inflammatory injury via c-Src tyrosine kinase pathway activation during mechanical ventilation.
CONCLUSIONS: Our results suggested that autophagy caused VILI via regulating c-Src activation, which implies that c-Src may serve as a promising therapeutic target in VILI. PMID: 30840292 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

ZEB1 causes the production of hsa-microRNA-99b/let-7e/microRNA-125a cluster and promotes invasion of liver cancer cells.
CONCLUSIONS: Hsa-microRNA-99b/let-7e/microRNA-125a cluster is highly-expressed in hepatocarcinoma, and its expression can be regulated by ZEB1. In addition, the overexpression of this cluster can promote the invasion of liver cancer cells and advance liver cancer progression. PMID: 30840268 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MEG3 promotes liver cancer by activating PI3K/AKT pathway through regulating AP1G1.
CONCLUSIONS: Low expression of MEG3 could promote the proliferative and invasive abilities of hepatoma cells and accelerate cell cycle. The mechanism may be related to the inhibition of AP1G1 expression and activation of PI3K/AKT pathway. PMID: 30840267 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation
This report suggests asprosin as a novel therapeutic target for the treatment of type 2 diabetes through preservation of β-cell function.Graphical abstract
Source: Molecular and Cellular Endocrinology - March 8, 2019 Category: Endocrinology Source Type: research

GSE124757 Phosphatase inhibitor PPP1R11 modulates resistance of human T cells towards Treg-mediated suppression of TCR signaling
Contributors : Sunjay J Fernandes ; Rubin N Joshi ; Angelika SchmidtSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe have performed RNAseq analysis of human T cells upon PPP1R11 siRNA silencing. We have explored the role of PPP1R11 for the first time in primary human T cells and discovered that PPP1R11 is a novel negative mediator of T cell activation.
Source: GEO: Gene Expression Omnibus - March 8, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

MicroRNA-370 protects against myocardial ischemia/reperfusion injury in mice following sevoflurane anesthetic preconditioning through PLIN5-dependent PPAR signaling pathway.
This study aims to investigate the effects of microRNA (miR-370) targeting perilipin-5 (PLIN5) in mice following sevoflurane anesthetic preconditioning (SAP). A mouse model of left ventricular myocardial IRI was established, followed by the evaluation of myocardial infarction size and cardiac function to determine the effects of SAP. The underlying regulatory mechanisms of miR-370 were analyzed in concert with the treatment of miR-370 mimic, miR-370 inhibitor, or siRNA against PLIN5 in cardiomyocytes isolated from mice with IRI. Also, cardiomyocyte proliferation, cell cycle distribution and apoptosis were evaluated followi...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 8, 2019 Category: Drugs & Pharmacology Authors: Zhao YB, Zhao J, Zhang LJ, Shan RG, Sun ZZ, Wang K, Chen JQ, Mu JX Tags: Biomed Pharmacother Source Type: research

Desmocollin 3 has a tumor suppressive activity through inhibition of AKT pathway in colorectal cancer.
In this study, we performed cell-based functional analysis after DSC3 overexpression by stable transfection and knockdown by siRNA in CRC cells. It turned out that overexpression of DSC3 reduced cell proliferation, colony forming ability, induced G0/G1 cell cycle arrest and promoted apoptosis. Further pathway analysis showed that overexpression of DSC3 significantly inhibited the activity of AKT pathway and increased the expression of E-cadherin as well as p53 and p21. In contrast, siRNA-mediated knockdown of DSC3 increased cell proliferation and colony formation, activated the AKT pathway and decreased the expression of E...
Source: Experimental Cell Research - March 8, 2019 Category: Cytology Authors: Cui T, Yang L, Ma Y, Petersen I, Chen Y Tags: Exp Cell Res Source Type: research

Diesel exhaust particles elevate MUC5AC and MUC5B expression via the TLR4-mediated activation of ERK1/2, p38 MAPK, and NF- κB signaling pathways in human airway epithelial cells.
Diesel exhaust particles elevate MUC5AC and MUC5B expression via the TLR4-mediated activation of ERK1/2, p38 MAPK, and NF-κB signaling pathways in human airway epithelial cells. Biochem Biophys Res Commun. 2019 Mar 08;: Authors: Na HG, Kim YD, Choi YS, Bae CH, Song SY Abstract Exposure to diesel exhaust particles (DEPs) is known to cause serious health problems, owing to a steady increase in the number of diesel vehicles worldwide. DEPs comprise approximately 90% particle mass existing in the fine size range (≤2.5 μm) and are mainly absorbed in the respiratory tract. However, limited information...
Source: Biochemical and Biophysical Research communications - March 8, 2019 Category: Biochemistry Authors: Na HG, Kim YD, Choi YS, Bae CH, Song SY Tags: Biochem Biophys Res Commun Source Type: research

IQGAP1 mediates podocyte injury in diabetic kidney disease by regulating nephrin endocytosis.
Abstract Diabetic kidney disease (DKD) is a complication associated with diabetes and is a major public health problem in modern society. Podocyte injury is the central target of the development of DKD, and the loss or dysregulation of nephrin, a key structural and signalling molecule located in the podocyte slit diaphragm (SD), initiates potentially catastrophic downstream events within podocytes. IQGAP1, a scaffold protein containing multiple protein-binding domains that regulates endocytosis, can interact with nephrin in podocytes. It is hypothesized that IQGAP1 contributes to nephrin endocytosis and may partic...
Source: Cellular Signalling - March 8, 2019 Category: Cytology Authors: Liu Y, Su H, Ma C, Ji D, Zheng X, Wang P, Zheng S, Wang L, Wang Z, Xu D Tags: Cell Signal Source Type: research

Hypoxia-inducible factor (HIF)-prolyl hydroxylase 3 (PHD3) maintains high HIF2A mRNA levels in clear cell renal cell carcinoma Cell Biology
Most clear cell renal cell carcinomas (ccRCCs) have inactivation of the von Hippel–Lindau tumor suppressor protein (pVHL), resulting in the accumulation of hypoxia-inducible factor α-subunits (HIF-α) and their downstream targets. HIF-2α expression is particularly high in ccRCC and is associated with increased ccRCC growth and aggressiveness. In the canonical HIF signaling pathway, HIF-prolyl hydroxylase 3 (PHD3) suppresses HIF-2α protein by post-translational hydroxylation under sufficient oxygen availability. Here, using immunoblotting and immunofluorescence staining, qRT-PCR, and siRNA-mediated gene silencing, we s...
Source: Journal of Biological Chemistry - March 8, 2019 Category: Chemistry Authors: Petra Miikkulainen, Heidi Hogel, Fatemeh Seyednasrollah, Krista Rantanen, Laura L. Elo, Panu M. Jaakkola Tags: Cell Biology Source Type: research

miR-203 inhibits the invasion and EMT of gastric cancer cells by directly targeting Annexin A4.
Authors: Li J, Zhang B, Cui J, Liang Z, Liu K Abstract Many studies have shown that down-regulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is down-regulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of Annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells was suppressed by overexpre...
Source: Oncology Research - March 7, 2019 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

FGF2-FGFR1 pathway activation together with thymidylate synthase upregulation is induced in pemetrexed-resistant lung cancer cells.
Authors: Miura K, Oba T, Hamanaka K, Ito KI Abstract Pemetrexed (MTA) is a folate antimetabolite used for treating non-small cell lung cancer. To elucidate the mechanisms of pemetrexed resistance in lung cancer, we established pemetrexed-resistant sublines in PC9 (mutant EGFR) and H1993 (wild-type EGFR) lung adenocarcinoma cell lines (PC9-MTA, H1993-MTA). Gene expression profile comparison by microarray analyses revealed enhanced fibroblast growth factor 2 (FGF2) and FGF receptor 1 (FGFR1) expression, confirmed by Western blotting, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reacti...
Source: Oncotarget - March 7, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Tripterygium glycoside protects diabetic kidney disease mouse serum-induced podocyte injury by upregulating autophagy and downregulating β-arrestin-1.
CONCLUSIONS: This study demonstrated that tripterygium glycoside provided protection against podocyte injury induced by high-glucose serum, and that this effect was mediated by the concomitant activation of autophagy and downregulation of β-arrestin-1. PMID: 30839094 [PubMed - as supplied by publisher]
Source: Histology and Histopathology - March 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

ASAP AIE Featured Inorganic –Organic Core@Shell Nanoparticles for High-Efficiency siRNA Delivery and Real-Time Monitoring
Nano LettersDOI: 10.1021/acs.nanolett.8b04677
Source: Nano Letters - March 7, 2019 Category: Nanotechnology Authors: Xuewen He, Feng Yin, Dongyuan Wang, Ling-Hong Xiong, Ryan T. K. Kwok, Peng Fei Gao, Zheng Zhao, Jacky W. Y. Lam, Ken-Tye Yong, Zigang Li, Ben Zhong Tang Source Type: research

Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.
Abstract Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize pathogen-associated molecular patterns to initiate downstream signal cascades to active immune responses. Our previous studies reported that, in vitro human cornea epithelium cells represented a novel target of TSLP and that TSLP/TSLPR/STAT5 signaling played an important role in the response t...
Source: Experimental Eye Research - March 7, 2019 Category: Opthalmology Authors: Dai C, Wu J, Chen C, Wu X Tags: Exp Eye Res Source Type: research

Identification of rigosertib for the treatment of recessive dystrophic epidermolysis bullosa-associated squamous cell carcinoma.
CONCLUSIONS: These data support a "first in RDEB" phase II clinical trial of rigosertib to assess tumor targeting in patients with late stage, metastatic and/ or unresectable SCC. PMID: 30846478 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - March 7, 2019 Category: Cancer & Oncology Authors: Atanasova VS, Pourreyron C, Farshchian M, Lawler ME, Brown CA, Watt S, Wright S, Warkala M, Guttmann-Gruber C, Pinon-Hofbauer J, Fuentes I, Prisco M, Rashidghamat E, Has C, Salas-Alanis JC, Palisson F, Hovnanian A, McGrath JA, Mellerio J, Bauer JW, South Tags: Clin Cancer Res Source Type: research

Regulation of Proliferation and Epithelial-to-Mesenchymal Transition (EMT) of Gastric Cancer by ZEB1 via Modulating Wnt5a and Related Mechanisms.
CONCLUSIONS ZEB1 expression is increased in GC tumor tissues and is associated with pathological features. The downregulation of ZEB1 can facilitate cell apoptosis via mediating Wnt5a, further suppressing GC cell proliferation and migration, and reducing EMT occurrence. PMID: 30829316 [PubMed - in process]
Source: Medical Science Monitor - March 6, 2019 Category: Research Tags: Med Sci Monit Source Type: research

SOCS3 ablation enhances DC-derived Th17 immune response against Candida albicans by activating IL-6/STAT3 in vitro
In this study, a si-RNA method is used to repress expression of the cytokine signaling-3 suppressor (SOCS3) in murine bone marrow-DCs, and then the maturation of DCs and the subsequent T-cell response after exposure to C. albicans are monitored in vitro.Key findingsAlong with a higher expression of the DC maturation markers CD40, CD86 and MHC-II, IL-6/STAT3 is markedly upregulated in the SOCS3 siRNA-treated DCs after exposure to C. albicans as compared with control DCs. In response to DCs maturation, CD4+ T cells have an increased expression of Th17 cell markers –– including the retinoic acid-related orphan nuclear hor...
Source: Life Sciences - March 6, 2019 Category: Biology Source Type: research

β-Hydroxybutyrate, a ketone body, reduces the cytotoxic effect of cisplatin via activation of HDAC5 in human renal cortical epithelial cells
In this study, we used human renal cortical epithelial (HRCE) cells. The anti-apoptotic effect of βOHB was evaluated using flow cytometry analysis. The expression of apoptosis-related proteins and HDACs was evaluated by western immunoblot.Key findingsThe results showed that βOHB significantly reduced cisplatin-induced apoptosis in HRCE cells. Furthermore, βOHB significantly reduced cisplatin-induced cleavage of caspase-3, acetylation of histone H3, and phosphorylation of AMP-activated kinase. This anti-apoptotic effect of βOHB was markedly attenuated by an inhibitor of HDAC4/5, and βOHB-mediated suppression of cleavag...
Source: Life Sciences - March 6, 2019 Category: Biology Source Type: research

EZH2 promotes gastric cancer cells proliferation by repressing p21 expression
In this study, immunohistochemical analysis was conducted to investigate the EZH2 expression in gastric cancer. We found that EZH2 levels were increased in gastric cancer tissues compared with adjacent normal tissues. Moreover, patients with high levels of EZH2 expression had a relatively poor prognosis. Furthermore, knockdown of EZH2 expression by siRNA could impair cell proliferation and invasion both in vitro and vivo. Finally, we found that EZH2 influences gastric cancer cells proliferation partly through regulating p21 expression. Our findings present that EZH2 over-expression can be identified as a poor prognostic bi...
Source: Pathology Research and Practice - March 6, 2019 Category: Pathology Source Type: research

The mechanism of miR-192 in regulating high glucose-induced MCP-1 expression in rat glomerular mesangial cells.
CONCLUSIONS: MiR-192 expression was upregulated to increase the expression of inflammatory factor MCP-1 by inhibiting the expression of Zeb1, which was mediated by breaking the regulatory loop of Zeb1 and miR-200b/c in rat mesangial cells cultured in high glucose. PMID: 30827272 [PubMed - as supplied by publisher]
Source: Endocrine, Metabolic and Immune Disorders Drug Targets - March 6, 2019 Category: Drugs & Pharmacology Tags: Endocr Metab Immune Disord Drug Targets Source Type: research

Cyclic Dinucleotides Inhibit Osteoclast Differentiation Through STING ‐Mediated Interferon‐β Signaling
In this study, we investigated the effect of CDNs on the differentiation and function of osteoclasts and osteoblasts. When bone marrow‐derived macrophages (BMMs) were differentiated into osteoclasts with macrophage colony‐stimulating factor (M‐CSF) and receptor activator of NF‐κB ligand (RANKL) in the presence of CDNs, the differentiation was inhibited by CDNs in a dose‐d ependent manner. In contrast, CDNs did not influence the differentiation of committed osteoclasts or osteoblast precursors. STING signaling pathway appeared to be critical for CDNs‐mediated inhibition of osteoclast differentiation since CDNs ...
Source: Journal of Bone and Mineral Research - March 6, 2019 Category: Orthopaedics Authors: Yeongkag Kwon, Ok ‐Jin Park, Jiseon Kim, Jae‐Ho Cho, Cheol‐Heui Yun, Seung Hyun Han Tags: Original Article Source Type: research

Activation of HO-1 protects placental cells function in oxidative stress via regulating ZO-1/occludin.
Abstract We previously confirmed that Nuclear factor erythroid 2-related factor-2 (Nrf2) and heme oxygenase (HO-1) play synergistic roles in the pathogenesis of preeclampsia. To further explore the function of HO-1 in the pathogenesis of preeclampsia, we established oxidative stress models respectively with human first-trimester trophoblast/simian virus (HTR8/SVneo) and human umbilical vein endothelial cells (HUVECs) and then assessed the effect of HO-1 on the two cell lines in oxidative stress conditions. The cell oxidative stress models were incubated with Hemin (an inducer of HO-1), then, the HTR8/SVneo cells w...
Source: Biochemical and Biophysical Research communications - March 6, 2019 Category: Biochemistry Authors: Li J, Zhou J, Tian B, Chu Y, Zhang N, Hu X, Wan X, Ye Y Tags: Biochem Biophys Res Commun Source Type: research

Modeling Pancreatic Cancer Dynamics with Immunotherapy.
This study concludes that mono-immunotherapy is unlikely to control the pancreatic cancer and combined immunotherapies between anti-TGF-[Formula: see text] and adoptive transfers of immune cells can prolong patient survival. We show through numerical explorations that how these two types of immunotherapies are scheduled is important to survival. Applying TGF-[Formula: see text] inhibition first followed by adoptive immune cell transfers can yield better survival outcomes. PMID: 30843136 [PubMed - as supplied by publisher]
Source: Bulletin of Mathematical Biology - March 6, 2019 Category: Bioinformatics Authors: Hu X, Ke G, Jang SR Tags: Bull Math Biol Source Type: research

Characterisation of deubiquitylating enzymes in the cellular response to high-LET ionising radiation and complex DNA damage
Complex DNA damage (CDD) formation, which increases with increasing linear energy transfer (LET), is a major contributor to the therapeutic effect of radiotherapy. However little is known of the enzymes and mechanisms that control the cellular response to CDD and co-ordinate its repair. Using siRNA screening of deubiquitylating enzymes, we identify major roles for USP6 and ultimately PARP-1 protein in regulating CDD repair and in promoting cell survival in response to high-LET radiation.
Source: International Journal of Radiation Oncology * Biology * Physics - March 6, 2019 Category: Radiology Authors: Rachel J. Carter, Catherine M. Nickson, James M. Thompson, Andrzej Kacperek, Mark A. Hill, Jason L. Parsons Tags: Biology Contribution Source Type: research

Camptothecin induces c-Myc- and Sp1-mediated hTERT expression in LNCaP cells: Involvement of reactive oxygen species and PI3K/Akt.
Abstract Camptothecin (CPT), a quinoline alkaloid isolated from Camptotheca acuminate, targets topoisomerase I, which is continuously expressed in cancer cells. However, the molecular mechanisms responsible for CPT-induced telomerase inhibition remain unclear. Unexpectedly, we found that CPT upregulates hTERT expression and concomitantly increases telomerase activity. However, transfection of hTERT-targeting siRNA had no effect on CPT-induced G2/M phase arrest, suggesting that CPT-induced telomerase activation was not related to G2/M phase arrest. CPT simultaneously increased Nrf2 expression and the level of intra...
Source: Food and Chemical Toxicology - March 6, 2019 Category: Food Science Authors: Dilshara MG, Jayasooriya RGPT, Choi YH, Kim GY Tags: Food Chem Toxicol Source Type: research

Metformin reduced NLRP3 inflammasome activity in Ox-LDL stimulated macrophages through adenosine monophosphate activated protein kinase and protein phosphatase 2A.
Abstract Metformin has been suggested to have cardiovascular protective effects. Previous researches showed that metformin activates Adenosine Monophosphate Activated Protein Kinase (AMPK) and Protein Phosphatase 2A (PP2A). This research aimed to elucidate whether and how metformin affects NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome activity in oxidized low-density lipoprotein (ox-LDL) stimulated macrophages. Macrophages were treated with ox-LDL and metformin in a continuous manner, and NLRP3 inflammasome activation was evaluated by detecting IL-1β and caspase-1 p10 release by ELISA and western blot...
Source: European Journal of Pharmacology - March 6, 2019 Category: Drugs & Pharmacology Authors: Zhang L, Lu L, Zhong X, Yue Y, Hong Y, Li Y, Li Y Tags: Eur J Pharmacol Source Type: research

lnc TINCR induced by NOD1 mediates inflammatory response in 3T3-L1 adipocytes.
CONCLUSIONS: lnc TINCR is a positive regulator of inflammation-induced insulin resistance presumably via activation of NOD1 signaling pathways. PMID: 30851423 [PubMed - as supplied by publisher]
Source: Gene - March 6, 2019 Category: Genetics & Stem Cells Authors: Fang YX, Zou Y, Wang GT, Huang SH, Zhou YJ, Zhou YJ Tags: Gene Source Type: research

Chronic administration of nano-sized PAMAM dendrimers in vivo inhibits EGFR-ERK1/2-ROCK signaling pathway and attenuate diabetes-induced vascular remodeling and dysfunction
This study showed chronic in vivo administration of PAMAM dendrimers in an animal model of diabetes inhibited EGFR-ERK1/2-ROCK signaling and improved diabetes-induced vascular remodeling and dysfunction in a dose, generation (G6 > G5) and surface chemistry-dependent manner (cationic> anionic> neutral). Thus, naked PAMAM dendrimers have the propensity to modulate key (e.g. EGFR) cell signaling cascades with associated pharmacological consequences in vivo that are dependent on their physicochemical properties. PAMAMs, alone or in combination with vasculoprotective agents, might therefore have a beneficial role in t...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 5, 2019 Category: Nanotechnology Source Type: research

GSE127828 Dynamic imbalance between cancer cell subpopulations induced by Transforming Growth Factor Beta (TGF- β) is associated with a DNA methylome switch methylation
Contributor : Hector Hernandez-VargasSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDistinct subpopulations of neoplastic cells within tumors, including hepatocellular carcinoma (HCC), display pronounced ability to initiate new tumors and induce metastasis. Recent evidence suggests that signals from transforming growth factor beta (TGF- β) may increase the survival of these so called tumor initiating cells leading to poor HCC prognosis. However, how TGF-β establishes and modifies the key features of these cell subpopulations is not fully understood. In the present report we describe the ...
Source: GEO: Gene Expression Omnibus - March 5, 2019 Category: Genetics & Stem Cells Tags: Methylation profiling by genome tiling array Homo sapiens Source Type: research

GSE127763 Dynamic imbalance between cancer cell subpopulations induced by Transforming Growth Factor Beta (TGF- β) is associated with a DNA methylome switch expression array
Contributor : Hector Hernandez-VargasSeries Type : Expression profiling by arrayOrganism : Homo sapiensDistinct subpopulations of neoplastic cells within tumors, including hepatocellular carcinoma (HCC), display pronounced ability to initiate new tumors and induce metastasis. Recent evidence suggests that signals from transforming growth factor beta (TGF- β) may increase the survival of these so called tumor initiating cells leading to poor HCC prognosis. However, how TGF-β establishes and modifies the key features of these cell subpopulations is not fully understood. In the present report we describe the differential DN...
Source: GEO: Gene Expression Omnibus - March 5, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE114235 Exosome release is regulated by mTORC1 via a Rab27A-dependent mechanism
Contributors : Wenchong Zou ; Xiaochun BaiSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensExosomes are small membrane-bound vesicles released into extracellular spaces by many types of cells. These nanovesicles carry proteins, mRNA and miRNA and are involved in cell waste management and intercellular communication. In the present study we show that exosome release, which leads to net loss of cellular membrane and protein content, is negatively regulated by mechanistic target of rapamycin complex 1 (mTORC1). We find that in cells and animal models exosome release is inhibited by su...
Source: GEO: Gene Expression Omnibus - March 5, 2019 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research

Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer.
In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and p...
Source: Bosnian Journal of Basic Medical Sciences - March 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

PCV2 replication promoted by oxidative stress is dependent on the regulation of autophagy on apoptosis
AbstractPorcine circovirus type 2 (PCV2) is an economically important swine pathogen but some extra trigger factors are required for the development of PCV2-associated diseases. By evaluating cap protein expression, viral DNA copies and the number of infected cells, the present study further confirmed that oxidative stress can promote PCV2 replication. The results showed that oxidative stress induced autophagy in PCV2-infected PK15 cells. Blocking autophagy with inhibitor 3-methyladenine or ATG5-specific siRNA significantly inhibited oxidative stress-promoted PCV2 replication. Importantly, autophagy inhibition significantl...
Source: Veterinary Research - March 5, 2019 Category: Veterinary Research Source Type: research

MicroRNA-183 inhibition exerts suppressive effects on diabetic retinopathy by inactivating BTG1-mediated PI3K/Akt/VEGF signaling pathway.
In this study, we demonstrated that miR-183 silencing inhibiting cell growth and tube formation in vascular endothelial cells of DR rats via the PI3K/Akt/VEGF signaling pathway by upregulating BTG1. PMID: 30835506 [PubMed - as supplied by publisher]
Source: Am J Physiol Endocri... - March 5, 2019 Category: Endocrinology Authors: Zhang ZZ, Qin XH, Zhang J Tags: Am J Physiol Endocrinol Metab Source Type: research

Non-invasive Use of Positron Emission Tomography to Monitor Diethyl maleate and Radiation-Induced Changes in System x C − Activity in Breast Cancer
Conclusion[18F]FASu is a specific system xC− PET radiotracer and as such it can be used to monitor system xC− activity due to OS. As such, [18F]FASu has the potential to be used in therapy response monitoring by PET. Further optimization is required forin vivo application.
Source: Molecular Imaging and Biology - March 5, 2019 Category: Molecular Biology Source Type: research

LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation.
Abstract Mice deficient for SIRT6 exhibit a severely shortened lifespan, growth retardation, and highly elevated LINE1 (L1) activity. Here we report that SIRT6-deficient cells and tissues accumulate abundant cytoplasmic L1 cDNA, which triggers strong type I interferon response via activation of cGAS. Remarkably, nucleoside reverse-transcriptase inhibitors (NRTIs), which inhibit L1 retrotransposition, significantly improved health and lifespan of SIRT6 knockout mice and completely rescued type I interferon response. In tissue culture, inhibition of L1 with siRNA or NRTIs abrogated type I interferon response, in add...
Source: Cell Metabolism - March 5, 2019 Category: Cytology Authors: Simon M, Van Meter M, Ablaeva J, Ke Z, Gonzalez RS, Taguchi T, De Cecco M, Leonova KI, Kogan V, Helfand SL, Neretti N, Roichman A, Cohen HY, Meer MV, Gladyshev VN, Antoch MP, Gudkov AV, Sedivy JM, Seluanov A, Gorbunova V Tags: Cell Metab Source Type: research

The Long Non-Coding RNA CPR Regulates Cardiomyocyte Proliferation and Cardiac Repair.
CONCLUSIONS: Together, our findings identified that CPR is a suppressor of cardiomyocyte proliferation, and it indicates that lncRNA(s) take parts in the regulation of cardiomyocyte proliferation and cardiac repair. Our study provides an lncRNA-based therapeutic strategy for effective cardiac repair and regeneration. PMID: 30832495 [PubMed - as supplied by publisher]
Source: Circulation - March 5, 2019 Category: Cardiology Authors: Ponnusamy M, Liu F, Zhang YH, Li RB, Zhai M, Liu F, Zhou LY, Liu CY, Yan KW, Dong YH, Wang M, Qian LL, Shan C, Xu S, Wang Q, Zhang YH, Li PF, Zhang J, Wang K Tags: Circulation Source Type: research

MAP kinase and autophagy pathways cooperate to maintain RAS mutant cancer cell survival Medical Sciences
Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...
Source: Proceedings of the National Academy of Sciences - March 5, 2019 Category: Science Authors: Chih-Shia Lee, Liam C. Lee, Tina L. Yuan, Sirisha Chakka, Christof Fellmann, Scott W. Lowe, Natasha J. Caplen, Frank McCormick, Ji Luo Tags: PNAS Plus Source Type: research

MicroRNA ‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells.
MicroRNA‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells. Oncol Rep. 2019 Feb 19;: Authors: Alcantara KMM, Garcia RL Abstract Inactivation of the tumor suppressor protein Merlin leads to the development of benign nervous system tumors in neurofibromatosis type2 (NF2). Documented causes of Merlin inactivation include deleterious mutations in the encoding neurofibromin2 gene (NF2) and aberrant Merlin phosphorylation leading to proteasomal degradation. Rare somatic NF2 mutations have also been detected in com...
Source: Oncology Reports - March 4, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

circRNA ‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.
circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis. Mol Med Rep. 2019 Feb 18;: Authors: Qu Y, Dou P, Hu M, Xu J, Xia W, Sun H Abstract Chondrocyte extracellular matrix‑related circular RNAs (circRNA‑CER) have been demonstrated to be involved in various diseases. However, its role in the development of human breast cancer is not clearly understood. The aims of the present study were to assess circRNA‑CER expression in paired cancer tissue and adjacent non‑tumor tissue from 24 patients with breast cancer, and to explore the roles and mechani...
Source: Molecular Medicine Reports - March 4, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid.
Authors: Wang J, Wang Y, Wang Z, Wang F, He J, Yang X, Xie W, Liu Y, Zhang Y Abstract The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. The w1/o/w2 MMEs were prepared by two-step emulsifications: the first step was to prepare primary emulsions (w1/o) by low energy emulsification (LEE); the second step was to obtain stable w1/o/w2 MMEs by self-emulsifying. An extensive formulation optimization process was undertaken. The final w1/o/w2 MMEs could be formed in ddH2O, phosphate buffer solution (PBS, pH 7.4) and 1640 culture ...
Source: Drug Delivery - March 4, 2019 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

A new Th-17 cytokine in hidradenitis suppurativa: antimicrobial and pro-inflammatory role of IL-26.
CONCLUSIONS: Our findings suggest that infection susceptibility in HS might be related to IL-26. Despite the role of bacteria remains controversial in HS, this paper supports that there is a defect of antimicrobial response in these patients. This article is protected by copyright. All rights reserved. PMID: 30829398 [PubMed - as supplied by publisher]
Source: The British Journal of Dermatology - March 4, 2019 Category: Dermatology Authors: Scala E, Di Caprio R, Cacciapuoti S, Caiazzo G, Fusco A, Tortorella E, Fabbrocini G, Balato A Tags: Br J Dermatol Source Type: research

miR-194 Inhibits Liver Cancer Stem Cell Expansion by Regulating RAC1 pathway.
Abstract Liver cancer stem cells (CSCs) contribute to tumorigenesis, progression, drug resistance and recurrence of hepatocellular carcinoma (HCC). However, the underlying mechanism for the propagation of liver CSCs remains unclear. Herein, we observed low expression of miR-194 in chemoresistant HCC cells. A remarkable decrease of miR-194 was detected in EpCAM or CD133-positive liver CSCs and CSC-enriched hepatoma spheres. Interference miR-194 facilitated liver CSCs expansion by enhancing the self-renewal of liver CSCs. While up-regulating miR-194 inhibited liver CSCs expansion by suppressing the self-renewal of l...
Source: Experimental Cell Research - March 4, 2019 Category: Cytology Authors: Ran RZ, Chen J, Cui LJ, Lin XL, Fan MM, Cong ZZ, Zhang H, Tan WF, Zhang QG, Zhang YJ Tags: Exp Cell Res Source Type: research

LncRNA MRAK048635_P1 is critical for vascular smooth muscle cell function and phenotypic switching in essential hypertension.
In conclusion, our findings reveal that decreased MRAK048635_P1 is probably an important factor for vascular remodeling by affecting VSMC cell function and phenotypic switching in essential hypertension. PMID: 30833363 [PubMed - as supplied by publisher]
Source: Bioscience Reports - March 4, 2019 Category: Biomedical Science Authors: Fang G, Qi J, Huang L, Zhao X Tags: Biosci Rep Source Type: research

VEGF antagonism attenuates cerebral ischemia/reperfusion-induced injury via inhibiting endoplasmic reticulum stress-mediated apoptosis.
Abstract Endoplasmic reticulum (ER) stress-mediated apoptosis pathway is considered to play a vital role in mediating stroke and other cerebrovascular diseases. Previous studies have showed that vascular endothelial growth factor (VEGF) antagonism reduced cerebral ischemic/reperfusion (CI/R) damage, but whether attenuation of ER stress-induced apoptosis is contributing to its mechanisms remains elusive. Our study aimed to investigate the protective effect of VEGF antagonism on CI/R-induced injury. First, oxygen-glucose deprivation and re-oxygenation (OGD/R) BEND3 cell model was constructed to estimate siRNA-VEGF o...
Source: Biological and Pharmaceutical Bulletin - March 2, 2019 Category: Drugs & Pharmacology Authors: Feng SQ, Zong SY, Liu JX, Chen Y, Xu R, Yin X, Zhao R, Li Y, Luo TT Tags: Biol Pharm Bull Source Type: research

Effect of modification of MTDH gene expression on colorectal cancer aggressiveness.
CONCLUSIONS: MTDH has an important role in promoting CRC aggravation. Also, inhibition of MTDH expression may attenuate the carcinogenic behavior of CRC cells. Furthermore, MTDH-associated NF-κB p65 signaling pathways may be involved in mediating the biological behavior of CRC. PMID: 30836117 [PubMed - as supplied by publisher]
Source: Gene - March 2, 2019 Category: Genetics & Stem Cells Authors: El-Ashmawy NE, El-Zamarany EA, Khedr EG, Abo-Saif MA Tags: Gene Source Type: research

eIF4E is a critical regulator of human papillomavirus (HPV)-immortalized cervical epithelial (H8) cell growth induced by nicotine.
Abstract Tobacco smoke is known as a cofactor in the development of cervical precancer and cancer caused by human papillomavirus (HPV). The main component in cigarette smoke, nicotine, can be concentrated more strongly in cervical mucus than in blood and it has been implicated as a cocarcinogen that promotes a serial of cancers development through multiple prosurvival pathways. Although the mechanisms of nicotine-induced cell proliferation have been well studied in some epithelial cells, the molecular mechanism of its action in cervical epithelial cells is still unclear. The aims of this study were to investigate ...
Source: Toxicology - March 2, 2019 Category: Toxicology Authors: Chen L, Wang H Tags: Toxicology Source Type: research

SNHG6 is upregulated in primary breast cancers and promotes cell cycle progression in breast cancer-derived cell lines
ConclusionsOur results indicate that lncRNA SNHG6 is involved in breast cancer development and may be considered as a potential biomarker for the diagnosis, prognosis and treatment of breast cancer.
Source: Cellular Oncology - March 2, 2019 Category: Cancer & Oncology Source Type: research