Query: siRNA

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Total 13814 results found since Jan 2013.

Targeted-gene silencing of BRAF to interrupt BRAF/MEK/ERK pathway synergized photothermal therapeutics for melanoma using a novel FA-GNR-siBRAF nanosystem
Publication date: July 2018Source: Nanomedicine: Nanotechnology, Biology and Medicine, Volume 14, Issue 5Author(s): Yujuan Zhang, Xuelin Zhan, Shanshan Peng, Ying Cai, Yu Shrike Zhang, Yanling Liu, Zhigang Wang, Yanrong Yu, Yifan Wang, Qiaofa Shi, Xiaoping Zeng, Keng Yuan, Nanjin Zhou, Rakesh Joshi, Meng Zhang, Zhuxu Zhang, Weiping MinAbstractMelanoma is significantly associated with mutant BRAF gene, a suitable target for siRNA-based anti-melanoma therapy. However, a tumor-specific delivery system is a major hurdle for clinical applications. Here, we developed a novel nano-carrier, FA-GNR-siBRAF for safe topical applicati...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 5, 2018 Category: Nanotechnology Source Type: research

LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3
Conclusions: Taken together, the key findings of the present study suggest that LINC00311 induces proliferation and inhibits apoptosis of osteoclasts via the regulation of the Notch signaling pathway by inhibiting DLL3 expression, ultimately demonstrating that LINC00311 and its target gene DLL3 may serve as independent factors in cases of osteoporosis.Cell Physiol Biochem 2018;47:2291 –2306
Source: Cellular Physiology and Biochemistry - July 5, 2018 Category: Cytology Source Type: research

A Preliminary Investigation of PVT1 on the Effect and Mechanisms of Hepatocellular Carcinoma: Evidence from Clinical Data, a Meta-Analysis of 840 Cases, and < b > < i > In Vivo < /i > < /b > Validation
Conclusions: We hypothesized thatPVT1 could affect the biological function of HCC cells via targetingmiR-424-5p and regulatingINCENP. Focusing on the new insight of thePVT1/miR-424-5p/INCENP axis, this study provides a novel perspective for HCC therapeutic strategies.Cell Physiol Biochem 2018;47:2216 –2232
Source: Cellular Physiology and Biochemistry - July 5, 2018 Category: Cytology Source Type: research

Spinal SHP2 Contributes to Exaggerated Incisional Pain in Adult Rats Subjected to Neonatal and Adult Incisions via PI3K
Publication date: 10 August 2018Source: Neuroscience, Volume 385Author(s): Xu Ding, Wei Yang, Xiao-Dan Liu, Xi Yang, Huan-Min Wang, Jun TaiAbstractNeonatal injury-induced exaggeration of pain hypersensitivity after adult trauma is a significant clinical challenge. However, the underlying mechanisms remain poorly understood. Growing evidence shows that spinal Src homology-2 domain-containing protein tyrosine phosphatase-2 (SHP2) contributes to chronic pain in adult rodents. Here we demonstrated that the phosphorylation and expression of SHP2 in synaptosomal fraction of the spinal dorsal horn are elevated in adult rats subje...
Source: Neuroscience - July 5, 2018 Category: Neuroscience Source Type: research

Lipoxin A4 and its analog suppress hepatocarcinoma cell epithelial-mesenchymal transition, migration and metastasis via regulating integrin-linked kinase axis
Publication date: July 2018Source: Prostaglandins & Other Lipid Mediators, Volume 137Author(s): Fen Xu, Xiaoyan Zhou, Jian Hao, Hua Dai, Jian Zhang, Yuanqiao He, Hua HaoAbstractEpithelial-mesenchymal Transition (EMT) and migration play an important role in tumor progression, and lipoxin (LX), the ‘stop signal’ for inflammation, has been studied in basic research for its anti-inflammatory or inflammatory pro-resolving properties. Here, in the in vitro experiment, we showed that LXA4 could inhibit the EMT and migration in phorbol myristate acetate (PMA) or activated conditioned medium (ACM)-stimulated Hep3B cells by down...
Source: Prostaglandins and Other Lipid Mediators - July 5, 2018 Category: Lipidology Source Type: research

Expression of AGPAT2, an enzyme involved in the glycerophospholipid/triacylglycerol biosynthesis pathway, is directly regulated by HIF-1 and promotes survival and etoposide resistance of cancer cells under hypoxia
In conclusion, our findings demonstrate that AGPAT2, which is mutated in patients with congenital generalized lipodystrophy and over-expressed in different types of cancer, is a direct transcriptional target of HIF-1, suggesting that upregulation of lipid storage by HIF-1 plays an important role in adaptation and survival of cancer cells under low oxygen conditions.Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - July 5, 2018 Category: Lipidology Source Type: research

Overcoming immune tolerance in chronic hepatitis B by therapeutic vaccination
Publication date: June 2018Source: Current Opinion in Virology, Volume 30Author(s): Claudia Dembek, Ulrike Protzer, Michael RoggendorfThe currently used nucleoside analogs (i.e. entecavir and tenofovir) with high barrier-to-resistance efficiently suppress viral replication, limit inflammation and reduce the sequelae of chronic hepatitis B, but cannot cure the disease and thus have to be applied long-term. Therapeutic vaccination as an approach to cure chronic hepatitis B has shown promising pre-clinical results, nevertheless the proof of its efficacy in clinical trials is still missing. This may be partially due to subopti...
Source: Current Opinion in Virology - July 5, 2018 Category: Virology Source Type: research

gga-miR-142-5p attenuates IRF7 signaling and promotes replication of IBDV by directly targeting the chMDA5′s 3′ untranslated region
Publication date: July 2018Source: Veterinary Microbiology, Volume 221Author(s): Wei Ouyang, Jing Qian, Qun-xing Pan, Jing-yu Wang, Xing-xia Xia, Xiao-li Wang, Yu-mei Zhu, Yong-shan WangAbstractChicken melanoma differentiation-associated gene 5 (chMDA5) is a key pattern recognition receptor (PRR) that recognizes RNA viral infections and initiates an antiviral innate immune response in chickens. MicroRNAs (miRNAs) are involved in the regulation of chMDA5 to sense RNA virus infection, but how it exerts antiviral activity against infectious bursal disease virus (IBDV) infection and regulates chMDA5 in chicken cells is unclear...
Source: Veterinary Microbiology - July 5, 2018 Category: Veterinary Research Source Type: research

Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cel...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5
In this study, we focused on the roles of OGG1 in histone modification. First, to study the effects of OGG1 on histone modification, the protein levels of symmetric dimethylation of histone H4 arginine-3 (H4R3me2s) were determined by western blot analysis following the knockdown or overexpression of OGG1. Second, the molecular mechanisms by which OGG1 regulates H4R3me2s were assessed by co-immunoprecipitation (CO-IP) assays in mouse embryonic fibroblast (MEF) wild-type (WT) and Ogg−/− cells. Finally, to verify the regulation of H4R3me2s by OGG1 on specific genes, chromatin immunoprecipitation (CHIP) was performed on ME...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation
Publication date: Available online 22 June 2018Source: Journal of Molecular BiologyAuthor(s): Ptissam Bergam, Johannes M. Reisecker, Zsófia Rakvács, Nóra Kucsma, Graça Raposo, Gergely Szakacs, Guillaume van NielAbstractGenetically inheritable pigmentation defects provide a unique opportunity to reveal the function of proteins contributing to melanogenesis. Dyschromatosis universalis hereditaria (DUH) is a rare pigmentary genodermatosis associated with mutations in the ABCB6 gene. Here we use optical and electron microscopy imaging combined with biochemical tools to investigate the localization and function of ABCB6 in ...
Source: Journal of Molecular Biology - July 5, 2018 Category: Molecular Biology Source Type: research

Lethal (2) giant larvae regulates pleural mesothelial cell polarity in pleural fibrosis
In this study, we observed that PMC polarity was lost in fibrotic pleura. Next we found increased Lethal (2) giant larvae (Lgl) bound with aPKC and PAR-6B competing against PAR-3A in PAR complex, which led to cell polarity loss. Then we demonstrated that Lgl1 siRNA prevented cell polarity loss in PMCs, and Lgl1 conditional knockout (ER-Cre+/−Lgl1flox/flox) attenuated pleural fibrosis in a mouse model. Our data indicated that Lgl1 regulates cell polarity of PMCs, inhibition of Lgl1 and maintenance of cell polarity in PMCs could be a potential therapeutic treatment approach for pleural fibrosis.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 5, 2018 Category: Molecular Biology Source Type: research

c-Abl phosphorylation of Yin Yang 1's conserved tyrosine 254 in the spacer region modulates its transcriptional activity
In conclusion, we demonstrate the novel role of c-Abl kinase in regulation of YY1's transcriptional activity, linking YY1 regulation with c-Abl tyrosine kinase signaling pathways.Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 5, 2018 Category: Molecular Biology Source Type: research

Modeling endophilin-mediated Aβ disposal in glioma cells
Publication date: Available online 4 July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Bing Sun, Ping Fan, Meijian Liao, Yaou ZhangAbstractAutophagy dysregulation has emerged in age-related neurological diseases (Ulland et al.; Matheoud et al.; Ashkenazi et al. [[1], [2], [3]]). Alzheimer Disease (AD), the most common progressive neurodegenerative disorder, is characterized by the accumulation of amyloid-β (Aβ) plaques caused by aberrant Aβ metabolism (Qiang et al.; Sevigny et al.; Ittner et al. [[4], [5], [6]]). Glia constitute the brain immune system and ingest extracellular Aβ ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 5, 2018 Category: Molecular Biology Source Type: research

ERK5/KLF2 activation is involved in the reducing effects of puerarin on monocyte adhesion to endothelial cells and atherosclerotic lesion in apolipoprotein E-deficient mice
Publication date: August 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Volume 1864, Issue 8Author(s): Yan Deng, Tingwen Lei, Hongmei Li, Xiaochuan Mo, Zhuting Wang, Hailong OuAbstractPuerarin has properties of anti-oxidation and anti-inflammation, which has been demonstrated protective effects in atherosclerosis and other cardiovascular diseases. However, the detail molecular mechanism still remains unclear. Here, we determined whether the atheroprotective effect of puerarin was by reducing monocyte adhesion and explored the underlying mechanism. The results showed that puerarin dose- and ti...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - July 5, 2018 Category: Molecular Biology Source Type: research

FOXO1 inhibition potentiates endothelial angiogenic functions in diabetes via suppression of ROCK1/Drp1-mediated mitochondrial fission
Publication date: July 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Volume 1864, Issue 7Author(s): Yundi Shi, Shengjun Fan, Di Wang, Tianru Huyan, Jinwen Chen, Jiyun Chen, Jing Su, Xin Li, Zhuofei Wang, Shiyu Xie, Caihong Yun, Xuejun Li, Lu TieAbstractDiabetes-induced endothelial cell (EC) dysfunction and neovascularization impairment constitute vascular complications with limited treatment regimens. Transcription factor FOXO1 is a key angiogenic regulator and plays a pathologic role in progression of diabetes. The present study was designed to determine the involvement of FOXO1 in impaired...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - July 5, 2018 Category: Molecular Biology Source Type: research

Deficiency of parkin suppresses melanoma tumor development and metastasis through inhibition of MFN2 ubiquitination
Parkin, a critical gene of Parkinson's disease, is involved in the development of numerous cancers. However, the effect of parkin deficiency on melanoma growth and metastasis has not been reported. We showed that the tumor size and number of surface lung metastases, and expression of tumor growth and metastasis marker proteins were significantly lower in parkin-KO mice than those observed in non-transgenic controls. In an in vitro study, we also showed that parkin siRNA inhibited cell growth and migration of B16F10 and SK-Mel-28 cells.
Source: Cancer Letters - July 5, 2018 Category: Cancer & Oncology Authors: Yong Sun Lee, Yu Yeon Jung, Mi Hee Park, In Jun Yeo, Hyung Sik Im, Kyung Tak Nam, Hae Deun Kim, Shin Kook Kang, Ju Koung Song, Yu Ri Kim, Dong-Young Choi, Pil-Hoon Park, Sang Bae Han, Jae Suk Yun, Jin Tae Hong Tags: Original Articles Source Type: research

Up-regulated lncRNA AFAP1-AS1 indicates a poor prognosis and promotes carcinogenesis of breast cancer.
CONCLUSION: Up-regulated lncRNA AFAP1-AS1 indicates a poor prognosis in breast cancer patients and regulated the breast cancer cells proliferation, apoptosis and metastasis. PMID: 29974352 [PubMed - as supplied by publisher]
Source: Breast Cancer - July 5, 2018 Category: Cancer & Oncology Authors: Ma D, Chen C, Wu J, Wang H, Wu D Tags: Breast Cancer Source Type: research

Silencing GOLPH3 gene expression reverses resistance to cisplatin in HT29 colon cancer cells via multiple signaling pathways.
Abstract Golgi phosphorylated protein (GOLPH)3 is overexpressed in colorectal cancer tissues and promotes the proliferation of colon cancer cells. A previous study by the authors demonstrated that GOLPH3 was associated with poor prognosis in colorectal cancer. However, the association between GOLPH3 gene overexpression and resistance to platinum-based drugs in colon cancer remains unknown. In the present study, the association between GOLPH3 overexpression and resistance of HT29 colon cancer cells to cisplatin and the mechanism underlying the development of chemoresistance were investigated. HT29 cells were divide...
Source: International Journal of Oncology - July 5, 2018 Category: Cancer & Oncology Authors: Zhou ZP, Wang LP, Hong ZS, Qiu CZ, Wang MZ, Chen ZX, Tang LF, Yu WS, Wang CX Tags: Int J Oncol Source Type: research

The PA-interacting host protein nucleolin acts as an antiviral factor during highly pathogenic H5N1 avian influenza virus infection
In this study, we further explored the role of NCL during highly pathogenic H5N1 avian influenza virus infection. We found that depletion of endogenous NCL in mammalian cells by siRNA targeting during H5N1 infection resulted in significantly increased viral polymerase activity, elevated viral mRNA, cRNA and vRNA synthesis, accelerated viral replication, and enhanced apoptosis and necrosis. Moreover, siRNA silencing of NCL significantly exacerbated the inflammatory response, resulting in increased secretion of IL-6, TNF- α, TNF-β, CCL-4, CCL-8, IFN-α, IFN-β and IFN-γ. Conversely, overexpression of NCL significantly dec...
Source: Archives of Virology - July 5, 2018 Category: Virology Source Type: research

Upregulation of lncRNA Sox2ot indicates a poor prognosis for patients with hepatocellular carcinoma and promotes cell invasion.
Authors: Sun J, Wei X, Xu L Abstract Long non-coding RNA Sox2 overlapping transcript (lncRNA Sox2ot) expression has been demonstrated to be upregulated in a number of types of tumor, and may act as an oncogene. The present study aimed to evaluate the clinical role of lncRNA Sox2ot and its association with the epithelial-mesenchymal transition in hepatocellular carcinoma (HCC). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine the expression of lncRNA Sox2ot in 86 cases of HCC tissues and adjacent non-tumor tissues. Kaplan-Meier and log-rank test was used to analyze th...
Source: Oncology Letters - July 4, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

ILK promotes angiogenic activity of mesenchymal stem cells in multiple myeloma.
Authors: Zhao W, Zhang X, Zang L, Zhao P, Chen Y, Wang X Abstract Angiogenic activity in solid tumors has been demonstrated to promote metastasis through the activation of certain proteins involved in the epithelial-mesenchymal transition-associated process. The molecular mechanism underlying multiple myeloma-induced angiogenesis involves angiogenic cytokines by plasma cells as well as their induction within the microenvironment. Integrin-linked kinase (ILK) is a highly evolutionarily conserved intracellular protein that was originally identified as an integrin-interacting protein, and extensive genetic and biochem...
Source: Oncology Letters - July 4, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Vitamin D and DDX4 regulate the proliferation and invasion of ovarian cancer cells.
Authors: Chen Y, Sun Z, Xu J, Wang P, Tang J, Shi X, Liu J, Ren F, Xu L Abstract Ovarian cancer is one of the most commonly occurring types of cancer and one of the most common causes of cancer-associated mortality in women. Diagnosis of ovarian cancer at an early stage is difficult due to the lack of specific symptoms. In the present study, it is demonstrated that active vitamin D treatment prohibited the proliferation and invasion of ovarian cancer cells, and the expression level of a germ cell specific marker DEAD (Asp-Glu-Ala-Asp)-box helicase 4 (DDX4), which is overexpressed in ovarian cancer, was downregulate...
Source: Oncology Letters - July 4, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

MiR-205 as predictive biomarker and adjuvant therapeutic tool in combination with trastuzumab.
Authors: Cataldo A, Piovan C, Plantamura I, D'Ippolito E, Camelliti S, Casalini P, Giussani M, Déas O, Cairo S, Judde JG, Tagliabue E, Iorio MV Abstract Trastuzumab is the standard treatment for HER2+ breast cancer (BC) patients, and even though it significantly improved their clinical outcome, 50% of them do not benefit from this drug and disease recurs, underlining the need of reliable predictive biomarkers and new therapeutic strategies. Strikingly, despite all the molecular analyses performed to identify the escape mechanisms behind this resistance, it still represents a question point. MiRNAs have been correl...
Source: Oncotarget - July 4, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

HMGA2 Modulates the TGF β/Smad, TGFβ/ERK and Notch Signaling Pathways in human Lens Epithelial-Mesenchymal Transition.
HMGA2 Modulates the TGFβ/Smad, TGFβ/ERK and Notch Signaling Pathways in human Lens Epithelial-Mesenchymal Transition. Curr Mol Med. 2018 Jul 04;: Authors: Hou M, Bao X, Luo F, Chen X, Liu L, Wu M Abstract Multiple signaling pathways contribute to lens fibrosis, where transforming growth factor beta (TGFβ)-mediated signaling plays a central role. Canonical TGFβ/Smad signaling predominates, and noncanonical TGFβ/extracellular signal-regulated kinase (ERK) signaling and Notch signaling contribute to TGFβ-triggered epithelial-mesenchymal transition (EMT) in lens epithelial cells (LECs). These three ...
Source: Current Molecular Medicine - July 4, 2018 Category: Molecular Biology Authors: Hou M, Bao X, Luo F, Chen X, Liu L, Wu M Tags: Curr Mol Med Source Type: research

CRIP1 promotes cell migration, invasion and epithelial-mesenchymal transition of cervical cancer by activating the Wnt/ β‑catenin signaling pathway
In conclusion, CRIP1 promotes cell migration and invasion, mediates EMT and activates the Wnt/β‑catenin signaling pathway in CC.
Source: Life Sciences - July 4, 2018 Category: Biology Source Type: research

TWEAK/Fn14 mediates atrial-derived HL-1 myocytes hypertrophy via JAK2/STAT3 signalling pathway.
In conclusion, TWEAK/Fn14 axis mediates HL-1 atrial myocytes hypertrophy partly through activation of the JAK2/STAT3 pathway. PMID: 29971943 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - July 4, 2018 Category: Molecular Biology Authors: Hao L, Ren M, Rong B, Xie F, Lin MJ, Zhao YC, Yue X, Han WQ, Zhong JQ Tags: J Cell Mol Med Source Type: research

Aggravated Intestinal Apoptosis by ClC-3 Deletion is Lethal to Mice Endotoxemia.
Abstract Our previous study found that ClC-3 chloride channel functioned differently in the vascular and intestinal inflammation, the loss of ClC-3 reduced vascular inflammation but exacerbated intestinal inflammation. To furtherly clarify the role of ClC-3 chloride channels in systemic inflammation, we used LPS-induced endotoxemia model to investigate the response of wild-type and ClC-3 knockout mice to systemic inflammation. The results showed that in the LPS-induced endotoxemia model, the mortality of mice with ClC-3 deletion was significantly higher than that of wild-type mice. The liver and lung inflammations...
Source: Cell Biology International - July 4, 2018 Category: Cytology Authors: Huang LY, Li YJ, Li PP, Li HC, Ma P Tags: Cell Biol Int Source Type: research

Bcl6 knockdown aggravates hypoxia injury in cardiomyocytes via the P38 pathway.
This study was designed to determine whether Bcl6 affects hypoxia-induced cardiomyocyte injury and, if so, to identify the underlying mechanism. To meet this aim, cardiomyocytes were exposed to hypoxia and Bcl6 siRNA was used to silence Bcl6 in cardiomyocytes. Bcl6 knockdown under physiological conditions caused increased oxidative stress, apoptosis, and expression of pro-inflammatory cytokines. Increased inflammatory response, oxidative stress, and apoptosis were observed after cells were exposed to hypoxia for 24 h. Bcl6 knockdown aggravated cardiomyocyte injury when exposed to hypoxia. Bcl6 knockdown increased P38 act...
Source: Cell Biology International - July 4, 2018 Category: Cytology Authors: Gu Y, Luo M, Li Y, Su Z, Wang Y, Chen X, Zhang S, Sun W, Kong X Tags: Cell Biol Int Source Type: research

Inhibition of CXCR4 regulates epithelial mesenchymal transition of NSCLC via the Hippo-YAP signaling pathway.
This study aims to investigate the effect of CXCR4 inhibition on epithelial-mesenchymal transition (EMT), invasion and migration of NSCLC cells via the Hippo-YAP pathway. QRT-PCR and Western blot were employed to detect CXCR4 expression in NSCLC cell lines. A549 and H1299 cells were treated with WZ811 (0, 10, 30, and 50µM), and A549 cells were also divided into the Control, WZ811, YAP siRNA, and WZ811 + YAP groups. Wound-healing, Transwell assay, immunofluorescent staining and a luciferase reporter gene assay were performed in this experiment. Compared with human bronchial epithelial (HBE) cells, CXCR4 expression was ...
Source: Cell Biology International - July 4, 2018 Category: Cytology Authors: Zheng CH, Chen XM, Zhang FB, Zhao C, Tu SS Tags: Cell Biol Int Source Type: research

Modeling endophilin-mediated A β disposal in glioma cells
Publication date: Available online 4 July 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Bing Sun, Ping Fan, Meijian Liao, Yaou Zhang Autophagy dysregulation has emerged in age-related neurological diseases (Ulland et al.; Matheoud et al.; Ashkenazi et al. [1–3]). Alzheimer Disease (AD), the most common progressive neurodegenerative disorder, is characterized by the accumulation of amyloid-β (Aβ) plaques caused by aberrant Aβ metabolism (Qiang et al.; Sevigny et al.; Ittner et al. [4–6]). Glia constitute the brain immune system and ingest extracellular Aβ for degradation via t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 4, 2018 Category: Molecular Biology Source Type: research

Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells
Publication date: Available online 4 July 2018 Source:Redox Biology Author(s): Chiang-Wen Lee, Cheng-Hsun Wu, Yao-Chang Chiang, Yuh-Lien Chen, Kuo-Ting Chang, Chu-Chun Chuang, I-Ta Lee Pseudomonas aeruginosa (P. aeruginosa) infection in the lung is common in patients with cystic fibrosis (CF). Intercellular adhesion molecule-1 (ICAM-1) is known to play a key role in lung inflammation. Acute inflammation and its timely resolution are important to ensure bacterial clearance and limit tissue damage. Carbon monoxide (CO) has been shown to exert anti-inflammatory effects in various tissues and organ systems. Here, we explored ...
Source: Redox Biology - July 4, 2018 Category: Biology Source Type: research

How does Hsp90 function in RNAi-dependent heterochromatin assembly?
Abstract Heat-shock protein 90 (Hsp90) was recently identified as a silencing factor required for RNA interference (RNAi)-dependent heterochromatin assembly in the fission yeast Schizosaccharomyces pombe. As Hsp90 is known to contribute to the formation of small RNA-containing effector complexes, it would be expected that Hsp90 is also involved in the RNAi pathway in fission yeast. However, upon investigation, we found it very difficult to determine how Hsp90 modulates RNAi-dependent heterochromatin assembly in the cell. A lack of detectable small interfering RNAs in hsp90 mutant cells prevented us from examining ...
Source: Current Genetics - July 4, 2018 Category: Genetics & Stem Cells Authors: Kato H, Okazaki K, Urano T Tags: Curr Genet Source Type: research

Expression of the Long Intergenic Non-Coding RNA (lincRNA) of the NED25 Gene Modulates the microRNA-125b, STAT3, Nitric Oxide, and Procalcitonin Signaling Pathways in Patients with Sepsis.
CONCLUSIONS Downregulation of the lincRNA of the NED25 gene was associated with sepsis in patients by modulating the signaling pathways downstream of miR-125b/STAT3/PCT/NO signaling pathway. PMID: 29962507 [PubMed - in process]
Source: Medical Science Monitor - July 3, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
AbstractCanine respiratory coronavirus (CRCoV), identified in 2003, is a member of theCoronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.
Source: Veterinary Research - July 3, 2018 Category: Veterinary Research Source Type: research

Effect of BCLAF1 on HDAC inhibitor LMK-235-mediated apoptosis of diffuse large B cell lymphoma cells and its mechanism.
In this study, we investigated the up-regulation of BCLAF1 through NF-κB signaling pathways in LMK-235, mediating the apoptosis of two diffuse large B-cell lymphoma cell lines, OCI-LY10 and OCI-LY3. Further studies showed that BCLAF1 expression was increased in DLBCL cells after treatment with the NF-κB inhibitor Bay11-7082. The combination of Bay11-7082 and siRNA si-HDAC4 significantly increased BCLAF1 expression and further increased apoptosis. These results indicate that BCLAF1 plays an important role in LMK-235-mediated apoptosis and may be a potential target for the treatment of diffuse large B-cell lymphoma. P...
Source: Cancer Biology and Therapy - July 3, 2018 Category: Cancer & Oncology Authors: Li X, He Z, Cheng B, Fang Q, Ma D, Lu T, Wei D, Kuang X, Tang S, Xiong J, Wang J Tags: Cancer Biol Ther Source Type: research

Molecules, Vol. 23, Pages 1614: Garcinol Enhances TRAIL-Induced Apoptotic Cell Death through Up-Regulation of DR5 and Down-Regulation of c-FLIP Expression
k Taeg Kyu Kwon Garcinol is a polyisoprenylated benzophenone derived from the Garcinia indica fruit that possess potential therapeutic effects such as inhibition of inflammation and tumor expansion. Here, we investigated whether garcinol induces TRAIL sensitization in renal carcinoma cells. Single treatment with garcinol or TRAIL did not effect on apoptosis. However, combined treatment with garcinol plus TRAIL significantly induced apoptosis in renal carcinoma (Caki, ACHN and A498), lung carcinoma (A549), and hepatoma (SK-Hep1) cells. In contrast, garcinol plus TRAIL did not alter cell viability in normal cells. Garc...
Source: Molecules - July 2, 2018 Category: Chemistry Authors: Seok Kim Seung Un Seo Kyoung-Jin Min Seon Min Woo Ju-Ock Nam Peter Kubatka Shin Kim Jong-Wook Park Taeg Kyu Kwon Tags: Article Source Type: research

Parathyroid hormone 1 ‑34 inhibits senescence in rat nucleus pulposus cells by activating autophagy via the m‑TOR pathway.
Parathyroid hormone 1‑34 inhibits senescence in rat nucleus pulposus cells by activating autophagy via the m‑TOR pathway. Mol Med Rep. 2018 Jun 27;: Authors: Wang XY, Jiao LY, He JL, Fu ZA, Guo RJ Abstract Osteoporosis is closely associated with intervertebral disc degeneration. While parathyroid hormone (PTH) 1‑34, which is an established drug used to treatosteoporosis, is thought to inhibit the disc degeneration associated with osteoporosis, the precise mechanism involved remains unclear. In the present study, primary Sprague‑Dawley rat nucleus pulposus cells (NPCs) were cultured, phenotyped...
Source: Molecular Medicine Reports - July 1, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

GSE99805 The Nonstop decay and the RNA silencing systems operate cooperatively in plants
Contributors : Istv án Szádeczky-Kardoss ; Tibor Csorba ; Andor Auber ; Anita Schamberger ; Tünde Nyikó ; János Taller ; Tamás I Orbán ; József Burgyán ; Dániel SilhavySeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thaliana ; Nicotiana benthamianaTranslation-dependent mRNA quality control systems protect the protein homeostasis of eukaryotic cells by eliminating aberrant transcripts and stimulating the decay of their protein products. Although these systems are intensively studied in animals, little is known about the translation-dependent quality control systems in plants....
Source: GEO: Gene Expression Omnibus - July 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Nicotiana benthamiana Source Type: research

GSE86172 BORIS knockdown in colorectal cancer cells HCT116 and Caco-2
Contributors : Yanmei Zhang ; Mengdie Fang ; Juan Ren ; Yongfei Song ; Haining Yuan ; Jianfei Fang ; Dongmei Yan ; Xiaoju WangSeries Type : Expression profiling by arrayOrganism : Homo sapiensBORIS expresses abnormally in colorectal cancer cells. Both the expression and the copy number of BORIS are remarkably higher in colorectal cancer cells than in normal colon or rectal cells. BORIS is potential diagnosis, prognosis or therapeutic target for colorectal cancer. To expand our view of the signaling pathway related with BORIS, altered gene expression by BORIS knockdown was assessed by microarray assay.To study the gene regu...
Source: GEO: Gene Expression Omnibus - July 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Angiotensin II Suppresses Rev-erb α Expression in THP-1 Macrophages via the Ang II Type 1 Receptor/Liver X Receptor α Pathway.
CONCLUSION: Ang II downregulates Rev-erbα expression in THP-1 macrophages via the AT1R/LXRα pathway. PMID: 29590657 [PubMed - indexed for MEDLINE]
Source: Physiological Reviews - July 1, 2018 Category: Physiology Authors: Wang S, Gu X, Zhang Q, Zhang X, Li Y, Yao Y, Yu B, Zhang Y Tags: Cell Physiol Biochem Source Type: research

Fibulin-1 interacts with Sex Hormone Binding Globulin and is linked to less aggressive estrogen-dependent breast cancers
In this study we investigated the expression of fibulins, their functional relationship with SHBG and involvement in behavior of estrogen-sensitive breast cancer. Main methods Gene expression of fibulins was performed by Real time-PCR on two estrogen-sensitive breast cancer cell lines, MCF-7 and T47D. Fibulin-1 protein expression and localization were determined by Western blot and immunofluorescence. SHBG interaction with-fibulin-1 was assessed by GST-pull down assay. MCF-7 cell growth and gene expression, after fibulin-1 silencing by siRNA, were evaluated. Finally, the expression of fibulin-1 was correlated to clinical a...
Source: Life Sciences - July 1, 2018 Category: Biology Source Type: research

Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine- γ-Lyase-Produced H2S.
This study investigated the protection of TFR against cerebral ischemia-reperfusion injury via cystathionine-γ-lyase- (CSE-) produced H2S mechanism. CSE-/- mice and CSE-siRNA-transfected rat were used. Relaxation of cerebral basilar artery (CBA), H2S, and CSE mRNA were measured. TFR significantly inhibited cerebral ischemia-reperfusion-induced abnormal neurological symptom and cerebral infarct in the normal rats and the CSE+/+ mice, but not in the CSE-/- mice, and the inhibition was markedly attenuated in CSE-siRNA-transfected rat; TFR elicited a significant vasorelaxation in rat CBA, and the relaxation was markedly atten...
Source: Evidence-based Complementary and Alternative Medicine - June 30, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion.
Abstract Upregulated gene 11 (URG11), a new gene upregulated by hepatitis B virus X protein, is involved in the development and progression of several tumors, including liver, stomach, lung, and colon cancers. However, the role of URG11 in prostate cancer remains yet to be elucidated. By determined expression in human prostate cancer tissues, URG11 was found significantly upregulated and positively correlated with the severity of prostate cancer, compared with that in benign prostatic hyperplasia tissues. Further, the mRNA and protein levels of URG11 were significantly upregulated in human prostate cancer cell lin...
Source: Biomed Res - June 30, 2018 Category: Research Authors: Pan B, Ye Y, Liu H, Zhen J, Zhou H, Li Y, Qu L, Wu Y, Zeng C, Zhong W Tags: Biomed Res Int Source Type: research

Construction and identification of a model for HJURP gene defect expression in human embryo villus cells.
CONCLUSION: A lentiviral shRNA expression vector targeting the HJURP gene was successfully constructed and may effectively silence the target gene at a cellular level, which provides a experimental model for the influence of HJURP gene expressing inhibition on human embryo villus cells multiplication and chromosome segregation. PMID: 29949288 [PubMed - in process]
Source: Clinical and Experimental Obstetrics and Gynecology - June 30, 2018 Category: OBGYN Tags: Clin Exp Obstet Gynecol Source Type: research

Role of the NRP-1-mediated VEGFR2-independent pathway on radiation sensitivity of non-small cell lung cancer cells.
CONCLUSIONS: We demonstrated that when VEGFR2 was inhibited, NRP-1 appeared to regulate RAD51 expression through the VEGFR2-independent ABL-1 pathway, consequently regulating radiation sensitivity. In addition, the combined inhibition of VEGFR2 and NRP-1 appears to sensitize cancer cells to radiation. PMID: 29777301 [PubMed - indexed for MEDLINE]
Source: Clinical Lung Cancer - June 30, 2018 Category: Cancer & Oncology Authors: Hu C, Zhu P, Xia Y, Hui K, Wang M, Jiang X Tags: J Cancer Res Clin Oncol Source Type: research

Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression.
Authors: Zhang L, Wei C, Ruan Y, Zhang Y, Zhou Y, Lei D Abstract The migration and invasion of vascular smooth muscle cells (VSMCs) caused by advanced aging play an important role in diffuse intimal thickening, facilitate adverse arterial remodeling and contribute to the initiation and progression of cardiovascular diseases. The inhibitory function of Buyang Huanwu decoction (BYHWD) has been found on aortic intimal hyperplasia and VSMC proliferation, but its effect on age-associated migration and invasion remains unknown. Here, we used an in vitro angiotensin II (Ang II)-induced senescence model to study the effect...
Source: BioScience Trends - June 30, 2018 Category: Biomedical Science Tags: Biosci Trends Source Type: research

CIDEC Is Involved in LPS-Induced Inflammation and Apoptosis in Renal Tubular Epithelial Cells
In conclusion, the results demonstrated that CIDEC/AMPK signaling pa thway played an important role in LPS-induced inflammation and epithelial cells apoptosis.
Source: Inflammation - June 30, 2018 Category: Allergy & Immunology Source Type: research

Knockdown of ferroportin accelerates erastin-induced ferroptosis in neuroblastoma cells.
CONCLUSIONS: These results suggested that knockdown of Fpn accelerated erastin-induced ferroptosis by increasing iron-dependent lipid ROS accumulation, highlighting Fpn as a potential therapeutic target site for neuroblastoma. Thus, Fpn inhibitors may provide new access for chemosensitization of neuroblastoma. PMID: 29949159 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 29, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Study on the function and mechanism of long non-coding RNA DMTF1v4 in the occurrence of colon cancer.
CONCLUSIONS: The expression of lncRNA DMTF1v4 is elevated in colon cancer tissues; lncRNA DMTF1v4 promotes colon cancer cell proliferation and migration, and inhibits apoptosis by downregulating the expression of p-ERK, p-JNK, and p-p38, thus affecting the progression of colon cancer. This will provide a basis for the development of new clinical treatments for colon cancer. PMID: 29949153 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 29, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research