Query: siRNA

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Activation of TGR5 promotes osteoblastic cell differentiation and mineralization
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Qingfeng Wang, Guoqiang Wang, Bin Wang, Huilin YangAbstractImpairment of normal osteoblast differentiation has been associated with bone loss-related disorders, such as osteoporosis. Takeda G-protein coupled receptor 5 (TGR5) has been identified as an important modulator of bile acid and energy homeostasis. Little information regarding the effects of TGR5 on osteoblastic bone formation and matrix mineralization has been reported before. In the current study, we found that TGR5 was expressed in osteoblast-like cell line MC3T3-E1 cells...
Source: Biomedicine and Pharmacotherapy - October 16, 2018 Category: Drugs & Pharmacology Source Type: research

CXCL12 modulates the radiosensitivity of cervical cancer by regulating CD44.
In conclusion, the present data demonstrated that CXCL12 served an important role in the radioresistance of cervical cancer, suggestinh a novel therapeutic target. PMID: 30320394 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - October 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

The renaissance of human skin organ culture: A critical reappraisal.
Abstract Human skin organ culture (hSOC) is a simple but highly instructive and clinically relevant skin research method. It has been used for decades to study the development, differentiation, and function as well as the response to wounding or test agents of intact human skin in the presence of its appendages and all resident cell populations. hSOC has also proven useful in toxicological and oncological studies and studies of skin aging (both chronological aging and photoaging), skin energy metabolism, skin immunology, pigmentation biology, and cutaneous (neuro-)endocrinology and neurobiology. The pathobiology a...
Source: Differentiation - October 16, 2018 Category: Research Authors: Zhou L, Zhang X, Paus R, Lu Z Tags: Differentiation Source Type: research

β-Integrin mediates LPS-induced coelomocyte apoptosis in sea cucumber Apostichopus japonicus via the integrin/FAK/caspase-3 signaling pathway.
In this study, a novel FAK was identified from A. japonicus (designated as AjFAK) by β-integrin (designated as AjITGB) -mediated GST-pull down assay. This interaction was further validated in the LPS-exposed coelomocytes through co-immunoprecipitation and immunofluorescence analyses. To investigate the functional role of AjFAK in AjITGB-mediated coelomocyte apoptosis, we cloned the full-length cDNA of AjFAK and characterized its relationship with AjITGB through real-time PCR. The mRNA expression levels of AjFAK exhibited consistent expression patterns with those of AjITGB in our previous work with 0.48- and 0.22-fold decr...
Source: Developmental and Comparative Immunology - October 16, 2018 Category: Allergy & Immunology Authors: Wang Z, Li C, Xing R, Shao Y, Zhao X, Zhang W, Guo M Tags: Dev Comp Immunol Source Type: research

Isoliquiritigenin-mediated p62/SQSTM1 induction regulates apoptotic potential through attenuation of caspase-8 activation in colorectal cancer cells.
Abstract Isoliquiritigenin (ISL) is a natural flavonoid that exhibits anticancer properties in various carcinoma cell types. However, the precise mechanism responsible for its anticancer activity has not been elucidated fully. In the present study, we examined ISL-mediated apoptotic mechanisms in colorectal cancer (CRC) cells. ISL induced apoptosis in human HCT-116 cells and caused marked induction of p62/SQSTM1 mRNA and protein expression. Similarly, ISL potently inhibited in vivo tumor growth and induced p62/SQSTM1 expression in xenograft tumor tissues. In a p62/SQSTM1 siRNA transfection study, ISL-induced p62/S...
Source: European Journal of Pharmacology - October 16, 2018 Category: Drugs & Pharmacology Authors: Jin H, Seo GS, Lee SH Tags: Eur J Pharmacol Source Type: research

Endometriotic inflammatory microenvironment induced by macrophages can be targeted by niclosamide.
Abstract Endometriosis causes severe chronic pelvic pain and infertility. We have recently reported that niclosamide treatment reduces growth and progression of endometriosis-like lesions and inflammatory signaling (NFĸB and STAT3) in a mouse model. In the present study, we examined further inhibitory mechanisms by which niclosamide affects endometriotic lesions using an endometriotic epithelial cell line, 12Z, and macrophages differentiated from a monocytic THP-1 cell line. Niclosamide dose dependently reduced 12Z viability, reduced STAT3 and NFĸB activity, and increased both cleaved caspase-3 and cleaved PARP....
Source: Biology of Reproduction - October 16, 2018 Category: Reproduction Medicine Authors: Sekulovski N, Whorton AE, Shi M, Ii JAM, Hayashi K Tags: Biol Reprod Source Type: research

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Abstract Low-density lipoprotein receptor-related protein 4 (LRP4) is a multi-functional protein implicated in bone, kidney and neurological diseases including Cenani-Lenz syndactyly (CLS), sclerosteosis, osteoporosis, congenital myasthenic syndrome and myasthenia gravis. Why different LRP4 mutation alleles cause distinct and even contrasting disease phenotypes remain unclear. Herein, we utilized the zebrafish model to search for pathways affected by a deficiency of LRP4. The lrp4 knockdown in zebrafish embryos exhibits cyst formations at fin structures and the caudal vein plexus, malformed pectoral fins, defectiv...
Source: Cellular and Molecular Life Sciences : CMLS - October 16, 2018 Category: Cytology Authors: Tian J, Shao J, Liu C, Hou HY, Chou CW, Shboul M, Li GQ, El-Khateeb M, Samarah OQ, Kou Y, Chen YH, Chen MJ, Lyu Z, Chen WL, Chen YF, Sun YH, Liu YW Tags: Cell Mol Life Sci Source Type: research

Transforming Growth Factor β1 (TGF-β1) Appears to Promote Coronary Artery Disease by Upregulating Sphingosine Kinase 1 (SPHK1) and Further Upregulating Its Downstream TIMP-1.
CONCLUSIONS TGF-β1 appears to promote CAD through the induction of cell apoptosis by upregulating SPHK1 expression and further upregulating its downstream TIMP-1. PMID: 30317247 [PubMed - in process]
Source: Medical Science Monitor - October 15, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Staphylococcus aureus alpha toxin activates Notch in vascular cells
AbstractStaphylococcus aureus infection is one of the leading causes of morbidity in hospitalized patients in the United States, an effect compounded by increasing antibiotic resistance. The secreted agent hemolysin alpha toxin (Hla) requires the receptor A Disintegrin And Metalloproteinase domain-containing protein 10 (ADAM10) to mediate its toxic effects. We hypothesized that these effects are in part regulated by Notch signaling, for which ADAM10 activation is essential. Notch proteins function in developmental and pathological angiogenesis via the modulation of key pathways in endothelial and perivascular cells. Thus, ...
Source: Angiogenesis - October 15, 2018 Category: Molecular Biology Source Type: research

The oncolytic virus H101 combined with GNAQ siRNA ‐mediated knockdown reduces uveal melanoma cell viability
Journal of Cellular Biochemistry, EarlyView.
Source: Journal of Cellular Biochemistry - October 15, 2018 Category: Biochemistry Authors: Yongyun Li, Jie He, Chun Qiu, Qingfeng Shang, Guanxiang Qian, Xianqun Fan, Shengfang Ge, Renbing Jia Source Type: research

Long non-coding RNA MALAT1 mediates hypoxia-induced pro-survival autophagy of endometrial stromal cells in endometriosis.
Abstract Endometriosis is a common gynecological disease characterized by diminished apoptosis, sustained ectopic survival of dysfunctional endometrial cells. Hypoxia has been implicated as a crucial microenvironmental factor that contributes to endometriosis. It has been reported that long non-coding RNA MALAT1 (lncRNA-MALAT1) highly expressed in endometriosis and up-regulated by hypoxia. Hypoxia may also induce autophagy, which might act as cell protective mechanism. However, the relationship between lncRNA-MALAT1 and autophagy under hypoxia conditions in endometriosis remains unknown. In the present study, we f...
Source: J Cell Mol Med - October 15, 2018 Category: Molecular Biology Authors: Liu H, Zhang Z, Xiong W, Zhang L, Du Y, Liu Y, Xiong X Tags: J Cell Mol Med Source Type: research

Regulation of eIF4F translation initiation complex by the peptidyl prolyl isomerase FKBP7 in taxane-resistant prostate cancer.
CONCLUSIONS: Targeting FKBP7 or the eIF4G-containing eIF4F translation initiation complex could be novel therapeutic strategies to eradicate taxane-resistant prostate cancer cells. PMID: 30322877 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - October 15, 2018 Category: Cancer & Oncology Authors: Garrido MF, Martin NJ, Bertrand M, Gaudin C, Commo F, El Kalaany N, Al Nakouzi N, Fazli L, Del Nery E, Camonis J, Perez F, Lerondel S, LE Pape A, Gleave ME, Loriot Y, Desaubry L, Vagner S, Fizazi K, Chauchereau A Tags: Clin Cancer Res Source Type: research

In-vitro inhibition of spring viremia of carp virus replication by RNA interference targeting the RNA-dependent RNA polymerase gene.
Abstract Spring viremia of carp, a fatal viral disease, is caused by the spring viremia of carp virus (SVCV) and can result in up to 70% mortalities in common carps and significant economic losses in several other cyprinid aquaculture. The present study aimed to investigate the possible control of SVCV replication in Epithelioma papulosum cyprini (EPC) cells using the RNA interference technology targeting the RNA-dependent RNA polymerase (L) gene of the SVCV that is essential for its replication. Three stealth small interfering RNA (siRNA) sequences were designed to target three different regions on the SVCV-L gen...
Source: Journal of Virological Methods - October 15, 2018 Category: Virology Authors: Marzouk Fouad A, Soliman H, Abdallah ESH, Ibrahim S, El-Matbouli M, Elkamel AA Tags: J Virol Methods Source Type: research

Effects of HMGA2 gene downregulation by siRNA on lung carcinoma cell migration in A549 cell lines
Journal of Cellular Biochemistry, EarlyView.
Source: Journal of Cellular Biochemistry - October 14, 2018 Category: Biochemistry Authors: Sanaz Naghizadeh, Behzad Mansoori, Ali Mohammadi, Hossein Samadi Kafil, Zohreh Mousavi, Ebrahim Sakhinia, Behzad Baradaran Source Type: research

Activator protein-1 contributes to the NaCl-induced expression of VEGF and PlGF in RPE cells.
Conclusions: The data indicate that AP-1 is activated in RPE cells in response to extracellular hyperosmolarity and mediates in part via the NaCl-induced expression of VEGF and PlGF, and secretion of PlGF. It is suggested that high consumption of dietary salt may exacerbate the angiogenic response of RPE cells in part via activation of AP-1. PMID: 30310263 [PubMed - in process]
Source: Molecular Vision - October 13, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research

Hypoxic stabilization of mRNA is HIF-independent but requires mtROS.
Conclusion: Overall these data suggest that hypoxic mRNA stabilization is independent of HIF transcriptional activity but requires mtROS. PMID: 30305827 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - October 13, 2018 Category: Biochemistry Authors: Fortenbery GW, Sarathy B, Carraway KR, Mansfield KD Tags: Cell Mol Biol Lett Source Type: research

Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney
ConclusionThese findings suggest that NaPi-IIc is important for the expression of NaPi-IIa (NaPi-4) protein in OK cells. Suppression of Npt2c may downregulate Npt2a function in HHRH patients.
Source: Clinical and Experimental Nephrology - October 13, 2018 Category: Urology & Nephrology Source Type: research

Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2.
Abstract Sulindac sulfone is a metabolite of sulindac, a non-steroidal anti-inflammatory drug (NSAID), without anti-inflammatory ability. However, sulindac sulfone has been reported to significantly reduce polyps in patients with colorectal adenomatous polyposis in clinical trials. Thus, sulindac sulfone is expected to be useful for the chemoprevention of neoplasia with few side effects related to anti-inflammatory ability. To date, the molecular targets of sulindac sulfone have not yet fully investigated. Therefore, in order to newly identify sulindac sulfone-binding proteins, we generated sulindac sulfone-fixed ...
Source: Biochemical and Biophysical Research communications - October 13, 2018 Category: Biochemistry Authors: Aono Y, Horinaka M, Iizumi Y, Watanabe M, Taniguchi T, Yasuda S, Sakai T Tags: Biochem Biophys Res Commun Source Type: research

NET-1 promotes invasion, adhesion and growth of hepatocellular carcinoma by regulating the expression of BAX, caspase 3, caspase 8 and BCL2.
Authors: Wang M, Sun X, Jiang Y, Tan Z Abstract Dysregulation of neuroepithelial transforming gene-1 (NET-1) has been shown in hepatocellular carcinoma (HCC) patients. We aimed to evaluate the influence of NET-1 on HCC invasion, adhesion and growth. In vitro cellular functional assays including invasion and adhesion were performed to evaluate the effects of knockdown and overexpression of NET-1. HCC cells were transplanted into nude mice, and tumor growth was assessed. BAX, caspase 3, caspase 8 and BCL2 protein levels were detected by western blot. After transfection with NET-1 siRNA, NET-1 positive ratio in HCC ce...
Source: Cellular and Molecular Biology - October 12, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Gp91 < sup > phox < /sup > (NOX < sub > 2 < /sub > ) in Activated Microglia Exacerbates Neuronal Damage Induced by Oxygen Glucose Deprivation and Hyperglycemia in an in Vitro Model
Conclusion: Knockdown of gp91phoxsiRNA significantly reduced oxidative stress and the inflammatory response, and alleviated neuronal damage after HG and OGD treatment in a rat neuronal-microglial co-culture model.Cell Physiol Biochem 2018;50:783 –797
Source: Cellular Physiology and Biochemistry - October 12, 2018 Category: Cytology Source Type: research

Participation of NADPH 4 oxidase in thyroid regulation
Publication date: Available online 11 October 2018Source: Molecular and Cellular EndocrinologyAuthor(s): Romina Oglio, Leonardo Salvarredi, Luciano Rossich, Silvia Copelli, Mario Pisarev, Guillermo Juvenal, Lisa ThomaszAbstractDifferent factors are involved in thyroid function and proliferation such as thyrotropin (TSH), insulin, growth factors, iodide, etc. TSH and IGF1/insulin increase proliferation rate and stimulate genes involved in thyroid differentiation. In the present study, we analyse the physiological regulation of NOX4 expression by TSH, insulin and iodine, and the role of NOX4 on thyroid genes expression. Diff...
Source: Molecular and Cellular Endocrinology - October 12, 2018 Category: Endocrinology Source Type: research

ASAP Precise Enzymatic Cleavage Sites for Improved Bioactivity of siRNA Lipo-Polyplexes
Bioconjugate ChemistryDOI: 10.1021/acs.bioconjchem.8b00585
Source: Bioconjugate Chemistry - October 12, 2018 Category: Biochemistry Authors: S ören Reinhard, Yanfang Wang, Sebastian Dengler, Ernst Wagner Source Type: research

microRNA-199a may be involved in the pathogenesis of lupus nephritis via modulating the activation of NF-κB by targeting Klotho
Publication date: November 2018Source: Molecular Immunology, Volume 103Author(s): Hong Ye, Bofeng Su, Haizhen Ni, Linlin Li, Xuduan Chen, Xiaohan You, Huidi ZhangAbstractKlotho is considered to have renal protective effect by prohibiting the activation of the nuclear factor (NF)-κB pathway, while the role of microRNA-199a (miR-199a)/Klotho in lupus nephritis (LN) is still unknown. A single dose of pristane (0.5 ml) was intraperitoneally injected into 8 weeks-old female mice to establish the LN model. MiR-199a mimic or miR-199a inhibitor, Klotho plasmid or Klotho siRNA, and miR-199a inhibitor plus si-Klotho were transfec...
Source: Molecular Immunology - October 12, 2018 Category: Allergy & Immunology Source Type: research

Cyclic peptide-based nanostructures as efficient siRNA carriers
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Source: Artificial Cells, Blood Substitutes, and Biotechnology - October 12, 2018 Category: Biotechnology Authors: Bijayananda Panigrahi Rohit Kumar Singh Sourav Mishra Dindyal Mandal Source Type: research

Metabolic Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Inhibition of HIF1 α and LDHA.
CONCLUSIONS: We show that under standard culture conditions, the HIF1α-lactate dehydrogenase A axis is aberrantly upregulated in hPSC-CMs, preventing their metabolic maturation. Chemical or siRNA inhibition of this pathway results in an appropriate metabolic shift from aerobic glycolysis to oxidative phosphorylation. This in turn improves metabolic and functional maturation of hPSC-CMs. These findings provide key insight into molecular control of hPSC-CMs' metabolism and may be used to generate more physiologically mature cardiomyocytes for drug screening, disease modeling, and therapeutic purposes. PMID: 30355156 [PubMed - in process]
Source: Circulation Research - October 12, 2018 Category: Cardiology Authors: Hu D, Linders A, Yamak A, Correia C, Kijlstra JD, Garakani A, Xiao L, Milan DJ, van der Meer P, Serra M, Alves PM, Domian IJ Tags: Circ Res Source Type: research

Combined blockade of activating ERBB2 mutations and ER results in synthetic lethality of ER+/HER2 mutant breast cancer.
CONCLUSIONS: ERBB2 mutations hyperactivate the HER3/PI3K/AKT/mTOR axis, leading to antiestrogen resistance in ER+ breast cancer. Dual blockade of the HER2 and ER pathways is required for the treatment of ER+/HER2 mutant breast cancers. PMID: 30314968 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - October 12, 2018 Category: Cancer & Oncology Authors: Croessmann S, Formisano L, Kinch L, Gonzalez-Ericsson PI, Sudhan DR, Nagy RJ, Mathew A, Bernicker EH, Cristofanilli M, He J, Cutler RE, Lalani AS, Miller VA, Lanman RB, Grishin N, Arteaga CL Tags: Clin Cancer Res Source Type: research

Physcion 8-O- β-glucopyranoside Exerts Anti-tumor Activity against Non-small Cell Lung Cancer by Targeting PPARγ.
This article is protected by copyright. All rights reserved. PMID: 30312015 [PubMed - as supplied by publisher]
Source: Anatomical Record - October 12, 2018 Category: Anatomy Authors: Du Y, Lv Z, Sun D, Li Y, Sun L, Zhou J Tags: Anat Rec (Hoboken) Source Type: research

HER2 decreases drug sensitivity of ovarian cancer cells via inducing stem cell-like property in a NF κB-dependent way.
In this study, we explored the effect of HER2 on cancer stem cells induction and drug sensitivity of ovarian cancer cell lines. Firstly, we found that HER2 overexpression (HER2 OE) induced, while HER2 knockdown (HER2 KD) decreased CD44+/CD24- population. Consistently, HER2 expression was closely correlated with the sphere formation efficiency (SFE) of ovarian cancer cells. Secondly, we found that NFκB inhibition by specific inhibitor JSH23 or siRNA targeting subunit p65 dramatically impaired the induction of ovarian cancer stem cells by HER2, indicating that NFκB mediated HER2-induced ovarian cancer stem cells. Thirdly, ...
Source: Bioscience Reports - October 12, 2018 Category: Biomedical Science Authors: Wang W, Gao Y, Hai J, Yang J, Duan S Tags: Biosci Rep Source Type: research

PGC-1 α-Mediated Mitochondrial Biogenesis is Involved in Cannabinoid Receptor 2 Agonist AM1241-Induced Microglial Phenotype Amelioration.
PGC-1α-Mediated Mitochondrial Biogenesis is Involved in Cannabinoid Receptor 2 Agonist AM1241-Induced Microglial Phenotype Amelioration. Cell Mol Neurobiol. 2018 Oct 12;: Authors: Ma L, Niu W, Lv J, Jia J, Zhu M, Yang S Abstract Cannabinoid type 2 receptor (CB2R) agonist AM1241 induces anti-inflammation by ameliorating microglial phenotypes, the mechanism, however, is still unknown. Peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) is a transcription protein which can regulate mitochondrial biogenesis, and the aim of this study is to investigate whether PGC-1α is involved in...
Source: Cellular and Molecular Neurobiology - October 12, 2018 Category: Cytology Authors: Ma L, Niu W, Lv J, Jia J, Zhu M, Yang S Tags: Cell Mol Neurobiol Source Type: research

Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting Nox4/NF- κB and GRP78/PERK Pathways in Human Aorta Endothelial Cells
This study aimed to determine the protecting effects of catalpol against homocysteine (HCY)-induced injuries in human aortic endothelial cells (HAECs) and uncover the underlying mechanisms: 1. HAECs were cultured with different concentrations of HCY (3  mM) and catalpol (7.5 μΜ, 15 μΜ, 30 μΜ) for 24 h. (1) The level of MDA and GSH as well as LDH release was measured with colorimetric assay. (2) Reactive oxygen species (ROS) were detected by flow cytometry analysis. (3) Western blotting analysis was performed to detect the expression of Nox4, p22phox, ICAM-1, MCP-1, VCAM-1, I κB, nucleus p65, p65 phosphorylation...
Source: Inflammation - October 12, 2018 Category: Allergy & Immunology Source Type: research

Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130 Gene Regulation
Heat shock protein 27 (HSP27) protects cells under stress. Here, we demonstrate that HSP27 also promotes cell cycle progression of MRC-5 human lung fibroblast cells. Serum starvation for 24 h induced G1 arrest in these cells, and upon serum refeeding, the cells initiated cell cycle progression accompanied by an increase in HSP27 protein levels. HSP27 levels peaked at 12 h, and transcriptional up-regulation of six G2/M-related genes (CCNA2, CCNB1, CCNB2, CDC25C, CDCA3, and CDK1) peaked at 24–48 h. siRNA-mediated HSP27 silencing in proliferating MRC-5 cells induced G2 arrest coinciding with down-regulation of these six gen...
Source: Journal of Biological Chemistry - October 12, 2018 Category: Chemistry Authors: Ah-Mee Park, Ikuo Tsunoda, Osamu Yoshie Tags: Cell Biology Source Type: research

Palmitoylation of caveolin-1 is regulated by the same DHHC acyltransferases that modify steroid hormone receptors Neurobiology
Palmitoylation is a reversible post-translational addition of a 16-carbon lipid chain involved in trafficking and compartmentalizing target proteins. It is important for many cellular functions, including signaling via membrane-localized estrogen receptors (ERs). Within the nervous system, palmitoylation of ERα is necessary for membrane surface localization and mediation of downstream signaling through the activation of metabotropic glutamate receptors (mGluRs). Substitution of the single palmitoylation site on ERα prevents its physical association with the integral membrane protein caveolin-1 (CAV1), required for the fo...
Source: Journal of Biological Chemistry - October 12, 2018 Category: Chemistry Authors: Katherine R. Tonn Eisinger, Kevin M. Woolfrey, Samuel P. Swanson, Stephen A. Schnell, John Meitzen, Mark Dell'Acqua, Paul G. Mermelstein Tags: Cell Biology Source Type: research

Inhibiting crosstalk between MET signaling and mitochondrial dynamics and morphology: a novel therapeutic approach for lung cancer and mesothelioma.
Abstract The receptor tyrosine kinase MET is frequently involved in malignant transformation and inhibiting its activity in MET-dependent cancers is associated with improved clinical outcomes. Emerging evidence also suggests that mitochondria play an essential role in tumorigenesis and Dynamin Related Protein (DRP1), a key component of the mitochondrial fission machinery, has emerged as an attractive therapeutic target. Here, we report that inhibiting MET activity with the tyrosine kinase inhibitor MGCD516 attenuates viability, migration, and invasion of non-small cell lung cancer (NSCLC) and malignant pleural mes...
Source: Cancer Biology and Therapy - October 12, 2018 Category: Cancer & Oncology Authors: Wang J, Mirzapoiazova T, Carol Tan YH, Pang KM, Pozhitkov A, Wang Y, Wang Y, Mambetsariev B, Wang E, Nasser MW, Batra SK, Raz D, Reckamp K, Kulkarni P, Zheng Y, Salgia R Tags: Cancer Biol Ther Source Type: research

Downregulation of NGAL is Required for the Inhibition of Proliferation and the Promotion of Apoptosis of Human Gastric Cancer MGC-803 Cells
Conclusion: NGAL gene silencing inhibits proliferation and promotes apoptosis of MGC-803 cells, which can provide a novel theory for treatment of gastric cancer.Cell Physiol Biochem 2018;50:694 –705
Source: Cellular Physiology and Biochemistry - October 11, 2018 Category: Cytology Source Type: research

Aldehyde dehydrogenase 2 deficiency promotes atherosclerotic plaque instability through accelerating mitochondrial ROS-mediated vascular smooth muscle cell senescence
This study was designed to examine the effect of ALDH2 deficiency on cholesterol diet-induced atherosclerotic plaque progression and plaque vulnerability in atherosclerosis-prone ApoE knockout (ApoE−/−) mice with a focus on foam cell formation in macrophages and senescence of vascular smooth muscle cells (VSMCs). Serum lipid profile, plaque progression, and plaque vulnerability were examined in ApoE−/− and ALDH2/ApoE double knockout (ALDH2−/−ApoE−/−) mice after high-cholesterol diet intake for 8 weeks. ALDH2 deficiency increased the serum levels of triglycerides while it decreased levels of total choleste...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - October 11, 2018 Category: Molecular Biology Source Type: research

YM155 sensitizes non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitors through the mechanism of autophagy induction
In this study, we showed that YM155 markedly enhanced the sensitivity of erlotinib to EGFR-TKI resistant NSCLC cell lines H1650 (EGFR exon 19 deletion and PTEN loss) and A549 (EGFR wild type and KRAS mutation) through inducing autophagy-dependent apoptosis and autophagic cell death. The effects of YM155 combined with erlotinib on apoptosis and autophagy inductions were more obvious than those of YM155 in combination with survivin knockdown by siRNA transfection, suggesting that YM155 induced autophagy and apoptosis in the NSCLC cells partially depend on survivin downregulation. Meanwhile, we found that the AKT/mTOR pathway...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - October 11, 2018 Category: Molecular Biology Source Type: research

SATB1 siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells
Source: OncoTargets and Therapy - October 10, 2018 Category: Cancer & Oncology Tags: OncoTargets and Therapy Source Type: research

Effects of Carbamazepine on the P-gp and CYP3A Expression Correlated with PXR or NF-κB Activity in the bEnd.3 Cells
Publication date: Available online 9 October 2018Source: Neuroscience LettersAuthor(s): Xian-Jin Ke, Yong-fei Cheng, Nian Yu, Qing DiAbstractDrug-resistant epilepsy (DRE) is present in 20-30% of all patients who develop epilepsy. Growing evidences demonstrated that glutamate released during seizures to increase the brain P-glycoprotein (P-gp) expression. Carbamazepine (CBZ) is known to influence the P-gp and cytochrome P450 (CYP) expression. However, the exact molecular mechanism is still unknown. We investigated that the effects of NF-κB and pregnane X receptor (PXR) activity on P-gp and CYP3A expression in mouse brain e...
Source: Neuroscience Letters - October 10, 2018 Category: Neuroscience Source Type: research

Knockdown of Fibromodulin Inhibits Proliferation and Migration of RPE Cell via the VEGFR2-AKT Pathway.
Conclusion: Depletion of FMOD selectively downregulated the expression of VEGF and VEGFR2 and inhibited the signaling pathway of AKT phosphorylation, which consequently inhibited the proliferation and migration of RPE Cell. PMID: 30298106 [PubMed]
Source: Journal of Ophthalmology - October 10, 2018 Category: Opthalmology Tags: J Ophthalmol Source Type: research

RNA-binding Protein UNR Promotes Glioma Cell Migration and Regulates the Expression of Ribosomal Protein L9
ConclusionsUNR could bind to the 3'UTR of PTEN and RPL9 in glioma cell lines, therefore promoting glioma cell migration and regulating the expression of RPL9. Here, we establish a link between UNR and RPL9 protein, which will provide new ideas for the further study of glioma.
Source: Chinese Medical Sciences Journal - October 10, 2018 Category: General Medicine Source Type: research

GSE113242 Dysfunction of circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulation of Aldh3a1 gene
In this study, we identified up-regulation of Aldehyde dehydrogenase 3a1 (Aldh3a1) gene in oncogenic transformed mouse embryo fibroblasts prepared from Per2-mutant (Per2m/m) mice, which were associated with the resistance against chemotherapeutic drugs. Co-expression of H-rasV12 and SV40 large T antigen induced malignant transformation of both wild-type and Per2m/m cells, but the cytotoxic effects of methotrexate, gemcitabine, etoposide, vincristine, and oxaliplatin were significantly alleviated in oncogenic transformed Per2m/m cells. Although introduction of oncogenes increased the expression of Aldh3a1 in both wild-type ...
Source: GEO: Gene Expression Omnibus - October 10, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference
Functional analysis of animal parasitic nematode genes is often quite challenging due to the unavailability of standardised in vitro culture conditions and lack of adequate tools to manipulate these genes. Theref...
Source: Parasites and Vectors - October 10, 2018 Category: Microbiology Authors: Magalla Bastian Chalitha Lakmal Somarathne, Yasanthi Illika Nilmini Silva Gunawardene, Naduvilath Vishvanath Chandrasekharan, Arjuna Nisantha Bandara Ellepola and Ranil Samantha Dassanayake Tags: Research Source Type: research

MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS
This study provided information for understanding the molecular mechanism of SHVV pathogenicity and a potential antiviral strategy against SHVV infection.
Source: Fish and Shellfish Immunology - October 9, 2018 Category: Biology Source Type: research

Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation
Survival of cochlear sensory epithelial cells may be regulated by inhibitor of differentiation-1 (Id1) and the N-methyl-D-aspartic acid (NMDA) receptor. However, it is unclear whether Id1 and the NMDA receptor are involved in the radiation-mediated survival of rat cochlear sensory epithelial cells. Here, we show that the percentage of apoptotic cells increased, the percentage of cells in the S phase decreased, Id1 mRNA and protein expression decreased and the NMDA receptor subtype 2B (NR2B) mRNA and protein level increased in OC1 cells after radiation. Cells infected with the Id1 gene exhibited higher Id1 mRNA and protein ...
Source: Audiology and Neurotology - October 9, 2018 Category: Audiology Source Type: research

Astroprincin (FAM171A1, C10orf38)
Our group originally found and cloned cDNA for a 98-kDa type 1 transmembrane glycoprotein of unknown function. Because of its abundant expression in astrocytes, it was called the protein astroprincin (APCN). Two thirds of the evolutionarily conserved protein is intracytoplasmic, whereas the extracellular domain carries two N-glycosidic side chains. APCN is physiologically expressed in placental trophoblasts, skeletal and hearth muscle, and kidney and pancreas. Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender projections, whereas knockdown of APCN expression by siRNA caused disappearance of actin stress fibers.
Source: American Journal of Pathology - October 9, 2018 Category: Pathology Authors: Tiina Rasila, Olga Saavalainen, Hesham Attalla, Petri Lankila, Caj Haglund, Erkki H ölttä, Leif C. Andersson Tags: Regular article Source Type: research

Serpina3n, dominantly expressed in female osteoblasts, suppresses the phenotypes of differentiated osteoblasts in mice.
In conclusion, we first found Serpina3n as the most female osteoblast-dominant gene. Serpina3n exerts a suppression of the osteoblast phenotypes such as Col1a1 expression and ALP activity in differentiated osteoblasts, which might partly explain sex differences of the osteoblast phenotypes in mice. PMID: 30304388 [PubMed - as supplied by publisher]
Source: Endocrinology - October 9, 2018 Category: Endocrinology Authors: Ishida M, Kawao N, Okada K, Tatsumi K, Sakai K, Nishio K, Kaji H Tags: Endocrinology Source Type: research

Astroprincin (FAM171A1, C10orf38): A regulator of human cell shape and invasive growth.
Abstract Our group originally found and cloned cDNA for a 98 kDa type 1 transmembrane glycoprotein of unknown function. Due to its abundant expression in astrocytes it was called the protein astroprincin (APCN). Two thirds of the evolutionarily conserved protein is intracytoplasmic whereas the extracellular domain carries two N-glycosidic side chains. APCN is physiologically expressed in placental trophoblasts, skeletal and hearth muscle, and kidney and pancreas. Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender projections whereas knockdown of APCN expression by siRNA caused disappe...
Source: The American Journal of Pathology - October 9, 2018 Category: Pathology Authors: Rasila T, Saavalainen O, Attalla H, Lankila P, Haglund C, Hölttä E, Andersson LC Tags: Am J Pathol Source Type: research

Propofol Regulates Neural Stem Cell Proliferation and Differentiation via Calmodulin-Dependent Protein Kinase II/AMPK/ATF5 Signaling Axis.
CONCLUSIONS: The results from study demonstrated that propofol inhibits the proliferation, differentiation, and migration of NSCs, and these effects are partially mediated by CaMkII/pS485/AMPK/ATF5 signaling pathway. PMID: 30303867 [PubMed - as supplied by publisher]
Source: Anesthesia and Analgesia - October 9, 2018 Category: Anesthesiology Authors: Liang C, Du F, Wang J, Cang J, Xue Z Tags: Anesth Analg Source Type: research

Astroprincin (FAM171A1, C10orf38): A regulator of human cell shape and invasive growth
Our group originally found and cloned cDNA for a 98 kDa type 1 transmembrane glycoprotein of unknown function. Due to its abundant expression in astrocytes it was called the protein astroprincin (APCN). Two thirds of the evolutionarily conserved protein is intracytoplasmic whereas the extracellular domain carries two N-glycosidic side chains. APCN is physiologically expressed in placental trophoblasts, skeletal and hearth muscle, and kidney and pancreas. Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender projections whereas knockdown of APCN expression by siRNA caused disappearance of actin stress fibres.
Source: American Journal of Pathology - October 9, 2018 Category: Pathology Authors: Tiina Rasila, Olga Saavalainen, Hesham Attalla, Petri Lankila, Caj Haglund, Erkki H ölttä, Leif C. Andersson Tags: Regular Article Source Type: research

Rscreenorm: normalization of CRISPR and siRNA screen data for more reproducible hit selection
Reproducibility of hits from independent CRISPR or siRNA screens is poor. This is partly due to data normalization primarily addressing technical variability within independent screens, and not the technical d...
Source: BMC Bioinformatics - August 20, 2018 Category: Bioinformatics Authors: Costa Bachas, Jasmina Hodzic, Johannes C. van der Mijn, Chantal Stoepker, Henk M. W. Verheul, Rob M. F. Wolthuis, Emanuela Felley-Bosco, Wessel N. van Wieringen, Victor W. van Beusechem, Ruud H. Brakenhoff and Ren ée X. de Menezes Tags: Methodology article Source Type: research