Query: siRNA

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Total 15336 results found since Jan 2013.

Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer.
CONCLUSION: HuR mediated overexpression of IAP1 significantly correlates with poor outcomes and early progression of pancreatic cancer. Further studies are needed to assess the underlying mechanisms. PMID: 30670910 [PubMed - in process]
Source: World Journal of Gastroenterology : WJG - January 14, 2019 Category: Gastroenterology Authors: Lukosiute-Urboniene A, Jasukaitiene A, Silkuniene G, Barauskas V, Gulbinas A, Dambrauskas Z Tags: World J Gastroenterol Source Type: research

Insulin ameliorates pulmonary edema through the upregulation of epithelial sodium channel via the PI3K/SGK1 pathway in mice with lipopolysaccharide ‑induced lung injury.
Insulin ameliorates pulmonary edema through the upregulation of epithelial sodium channel via the PI3K/SGK1 pathway in mice with lipopolysaccharide‑induced lung injury. Mol Med Rep. 2019 Jan 02;: Authors: Deng W, Li CY, Tong J, He J, Zhao Y, Wang DX Abstract Epithelial sodium channel (ENaC) provides the driving force for the removal of edema from the alveolar spaces in acute lung injury (ALI). Our previous study reported that insulin increased the expression of α‑ENaC, possibly via the serum/glucocorticoid‑inducible kinase‑1 (SGK1) pathway in ALI; however, the upstream regulator of SGK1 activi...
Source: Molecular Medicine Reports - January 12, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Galectin-3 regulates chemotherapy sensitivity in epithelial ovarian carcinoma via regulating mitochondrial function.
In conclusion, Galectin-3 reduces the sensitivity of ovarian cancer cells to cisplatin via regulating cisplatin-induced mitochondrial dysfunction. Galectin-3 knockdown inhibits the chemo-resistance of EOC cells. It implies that Galectin-3 might be a potential target to overcome the chemo-resistance in EOC cells. PMID: 30626779 [PubMed - in process]
Source: Journal of Toxicological Sciences - January 12, 2019 Category: Toxicology Tags: J Toxicol Sci Source Type: research

GSE124934 Gene expression altered by HOTAIR knockdown in MCF-7-TNR
Contributors : Bin Shan ; Simon AlsagerSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe human HOTAIR-specific siRNA and control siRNA were transfected into MCF-7-TNR cells. RNA was collected at 72 hrs after transfection. RNA-SEQ was carried out to profile the gene expression altered by HOTAIR knockdown.
Source: GEO: Gene Expression Omnibus - January 11, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

The Hippo-YAP Pathway Regulates the Proliferation of Alveolar Epithelial Progenitors after Acute Lung Injury.
Abstract Regeneration of pulmonary epithelial cells plays an important role in the recovery of acute lung injury (ALI) which is defined by pulmonary epithelial cell death. However, the mechanism of the regenerative capacity of alveolar epithelial cells is unknown. Using a lung injury mouse model induced by hemorrhagic shock and LPS, a protein mass spectrometry-based high-throughput screening and linage tracing technology to mark alveolar epithelial type 2 cells (AEC2s), we analyzed the mechanism of alveolar epithelial cells proliferation. We demonstrated that the expression of Hippo-YAP1 (Yes-associated protein 1)...
Source: Cell Biology International - January 11, 2019 Category: Cytology Authors: Hu C, Sun J, Du J, Wen D, Lu H, Zhang H, Xue Y, Zhang A, Yang C, Zeng L, Jiang J Tags: Cell Biol Int Source Type: research

Suppression of tumor growth via IGFBP3 depletion as a potential treatment in glioma.
CONCLUSIONSOur findings suggest IGFBP3 predicts poor outcome of glioma patients and is a potential therapeutic target for which depletion of its expression suppresses tumor growth through inducing apoptosis and accumulation of DNA damage in glioma cells. PMID: 30641835 [PubMed - as supplied by publisher]
Source: Journal of Neurosurgery - January 11, 2019 Category: Neurosurgery Authors: Chen CH, Chen PY, Lin YY, Feng LY, Chen SH, Chen CY, Huang YC, Huang CY, Jung SM, Chen LY, Wei KC Tags: J Neurosurg Source Type: research

Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma
ConclusionsClptm1 and CRR9 were overexpressed in many OSCC tissues. In particular, CRR9 expression may promote tumor development and have a significant poor prognostic value in OSCC, possibly through conferring invasion ability and resistance to apoptotic stimuli possibly related to Bcl-xL expression. CRR9 could be a novel molecular target for patients with OSCC.
Source: Journal of Cancer Research and Clinical Oncology - January 11, 2019 Category: Cancer & Oncology Source Type: research

Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling
Positive-sense RNA viruses hijack intracellular membranes that provide niches for viral RNA synthesis and a platform for interactions with host proteins. However, little is known about host factors at the interface between replicase complexes and the host cytoplasm. We engineered a biotin ligase into a coronaviral replication/transcription complex (RTC) and identified>500 host proteins constituting the RTC microenvironment. siRNA-silencing of each RTC-proximal host factor demonstrated importance of vesicular trafficking pathways, ubiquitin-dependent and autophagy-related processes, and translation initiation factors. No...
Source: eLife - January 11, 2019 Category: Biomedical Science Tags: Microbiology and Infectious Disease Source Type: research

Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPAR δ-AMPK Pathway.
Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway. Evid Based Complement Alternat Med. 2018;2018:9616574 Authors: Lee YJ, Jang YN, Han YM, Kim HM, Jin C, Kim HJ, Seo HS Abstract Aster glehni (AG) has been used in cooking and as a medicine to treat various diseases for over hundreds of years in Korea. To speculate the protective effects of AG on skin barrier, we estimated the protein levels of biomarkers related to skin barrier protection in human keratinocytes, HaCaT cells treated with sodium dodecyl sulfate (SDS), or 2,4-dinitro...
Source: Evidence-based Complementary and Alternative Medicine - January 10, 2019 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

A Novel Antimicrobial Peptide-Derived Vehicle for Oligodeoxynucleotide Delivery to Inhibit TNF-α Expression
This study provides a useful strategy of peptide vehicle design, which may facilitate the delivery of not only ODN but also other oligonucleotides, including siRNA and miRNA, to promote gene silence application.Graphical abstract
Source: International Journal of Pharmaceutics - January 10, 2019 Category: Drugs & Pharmacology Source Type: research

ASAP Peptide-Based Nanoparticles to Rapidly and Efficiently “Wrap ’n Roll” siRNA into Cells
Bioconjugate ChemistryDOI: 10.1021/acs.bioconjchem.8b00776
Source: Bioconjugate Chemistry - January 10, 2019 Category: Biochemistry Authors: Karidia Konate, Marion Dussot, Gudrun Aldrian, Ana ïs Vaissière, Véronique Viguier, Isabel Ferreiro Neira, Franck Couillaud, Eric Vivès, Prisca Boisguerin, Sébastien Deshayes Source Type: research

Tumor-suppressive effects of microRNA-181d-5p on non-small-cell lung cancer through the CDKN3-mediated Akt signaling pathway in vivo and in vitro.
In conclusion, this study highlights the tumor suppressive effects of miR-181d-5p on NSCLC via Akt signaling pathway inactivation by suppressing CDKN3, providing a promising therapeutic strategy for the treatment of NSCLC. PMID: 30628487 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 10, 2019 Category: Cytology Authors: Gao LM, Zheng Y, Wang P, Zheng L, Zhang WL, Di Y, Chen LL, Yin XB, Tian Q, Shi SS, Xu SF Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Upregulation of MTA1 expression by human papillomavirus infection promotes CDDP resistance in cervical cancer cells via modulation of NF- κB/APOBEC3B cascade
ConclusionsThese findings reveal an obligatory coregulatory role of MTA1 in the indirect regulation of APOBEC3B expression via classical NF- κB pathway, and also suggest that inhibition of MTA1/NF-κB/APOBEC3B cascade may be repositioned to suppress cancer mutagenesis, dampen tumor evolution, and decrease the probability of adverse outcomes from CDDP resistance in CCa.
Source: Cancer Chemotherapy and Pharmacology - January 10, 2019 Category: Cancer & Oncology Source Type: research

Mst1 facilitates hyperglycemia-induced retinal pigmented epithelial cell apoptosis by evoking mitochondrial stress and activating the Smad2 signaling pathway.
Abstract Hyperglycemia induces retinal pigmented epithelial cell apoptosis and mitochondrial stress via poorly understood mechanisms. The goal of our current study is to explore whether mammalian sterile 20-like kinase 1 (Mst1) is involved in the pathogenesis of hyperglycemia-mediated retinal pigmented epithelial cell apoptosis by triggering mitochondrial abnormalities and activating the Smad2 signaling pathway. Retinal pigmented epithelial ARPE-19 cells were presented with a high-glucose challenge in vitro. Cell viability and apoptosis were measured via western blotting, ELISAs, and immunofluorescence assays. Mit...
Source: Cell Stress and Chaperones - January 10, 2019 Category: Cytology Authors: Wei B, Wang M, Hao W, He X Tags: Cell Stress Chaperones Source Type: research

Increased Dynamin ‐Related Protein 1‐Dependent Mitochondrial Fission Contributes to High‐Fat Diet‐Induced Cardiac Dysfunction and Insulin Resistance by Elevating Tafazzin in Mouse Hearts
ConclusionIn high ‐fat diet‐fed mouse hearts, increased tafazzin contributes to insulin resistance via mediating Drp‐1 translocation to the mitochondria, and a small non‐coding RNA, miR‐125b‐5p, at least partially regulates this signalling pathway and alleviates insulin resistance.This article is protected by copyright. All rights reserved
Source: Molecular Nutrition and Food Research - January 10, 2019 Category: Food Science Authors: Wenguang Chang, Dandan Xiao, Xiang Ao, Mengyang Li, Tao Xu, Jianxun Wang Tags: Research Article Source Type: research

Impact of dynamin 2 on adenovirus nuclear entry.
Abstract The large GTPase dynamin 2 controls both endosomal fission and microtubule acetylation. Here we report that dynamin 2 alters microtubules and regulates the trafficking of human adenovirus type 37. Dynamin 2 knockdown by siRNA in infected cells resulted in accumulation of acetylated tubulin, repositioning of microtubule organizing centers (MTOCs) closer to cell nuclei, increased virus in the cytosol (with a compensatory decrease in endosomal virus), reduced proinflammatory cytokine induction, and increased binding of virus to the nucleoporin, Nup358. These events led to increased viral DNA nuclear entry an...
Source: Virology - January 10, 2019 Category: Virology Authors: Lee JS, Ismail AM, Lee JY, Zhou X, Materne EC, Chodosh J, Rajaiya J Tags: Virology Source Type: research

Molecular cloning and preliminary functional analysis of six RING-between-ring (RBR) genes in grass carp (Ctenopharyngodon idellus)
Publication date: April 2019Source: Fish & Shellfish Immunology, Volume 87Author(s): Lifei Luo, Denghui Zhu, Rong Huang, Lv Xiong, Rumana Mehjabin, Libo He, Lanjie Liao, Yongming Li, Zuoyan Zhu, Yaping WangAbstractUbiquitination is a post-translational modification of proteins that is widely present in eukaryotic cells. There is increasing evidence that ubiquitinated proteins play crucial roles in the immune response process. In mammals, RING-between-RING (RBR) proteins play a key role in regulating immune signaling as the important E3 ubiquitin ligases during ubiquitination. However, the function of RBR in fish is still u...
Source: Fish and Shellfish Immunology - January 9, 2019 Category: Biology Source Type: research

IL-16 regulates macrophage polarization as a target gene of mir-145-3p
ConclusionIL-16 modulates macrophage polarization through regulating IL-10, IL-1a and IL-6 expression. Mir-145 is involved in M2 macrophage polarization by targeting IL-16 and enhancing IL-10 expression.
Source: Molecular Immunology - January 9, 2019 Category: Allergy & Immunology Source Type: research

Downregulated spinal IRF8 and BDNF in NAC are involved in neuropathic pain-induced depression relief via pulsed radiofrequency on dorsal root ganglion in rat SNI model.
This study was designed to explore the effect of PRF on DRG on the neuropathic pain-induced depression in rats with spared nerve injury (SNI). Here, we found that PRF on DRG or intrathecal injection of the interferon regulatory factor 8 (IRF8) siRNA prevented the increase of mechanical allodynia and depression-like behaviors of rats after receiving SNI. Meanwhile, Western blot, immunohistochemistry, and RT-PCR revealed that PRF on DRG or intrathecal injection of IRF8 siRNA inhibited IRF8 overexpression in the spinal cord and brain-derived neurotrophic factor (BDNF) in NAc. These results suggest that neuropathic pain-induce...
Source: Brain Research Bulletin - January 9, 2019 Category: Neurology Authors: Fang X, Xu X, Lin X, Liu R Tags: Brain Res Bull Source Type: research

AT1 Receptors in the Subfornical Organ Modulate Arterial Pressure and the Baroreflex In Two-kidney One-clip Hypertensive Rats.
Abstract The subfornical organ (SFO), a forebrain circumventricular organ that lies outside the blood brain barrier, has been implicated in arterial pressure and baroreflex responses to angiotensin II (Ang II). We tested whether pharmacologic inhibition or selective silencing of SFO AT1 receptors (AT1R) of two-kidney one-clip rats with elevated plasma Ang II decreases resting arterial pressure and renal sympathetic nerve activity (RSNA) and/or modulates arterial baroreflex responses of heart rate and RSNA. Male Sprague Dawley rats underwent renal artery clipping (2K-1C) or sham-clipping (Sham). Six weeks later, co...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - January 9, 2019 Category: Physiology Authors: Rossi NF, Zenner Z, Rishi AK, Levi E, Maliszewska-Scislo M Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research

Hepatic miR-181b-5p Contributes to Glycogen Synthesis Through Targeting EGR1
ConclusionWe demonstrated that miR-181b-5p contributes to glycogen synthesis by targeting EGR1, thereby regulating PTEN expression to mediate hepatic insulin resistance.
Source: Digestive Diseases and Sciences - January 9, 2019 Category: Gastroenterology Source Type: research

Molecules, Vol. 24, Pages 225: S-Acyl-2-Thioethyl: A Convenient Base-Labile Protecting Group for the Synthesis of siRNAs Containing 5 ′-Vinylphosphonate
Molecules, Vol. 24, Pages 225: S-Acyl-2-Thioethyl: A Convenient Base-Labile Protecting Group for the Synthesis of siRNAs Containing 5′-Vinylphosphonate Molecules doi: 10.3390/molecules24020225 Authors: Mehran Nikan Wenyu Li Garth A. Kinberger Punit P. Seth Eric E. Swayze Thazha P. Prakash We recently reported that (E)-5′-vinylphosphonate (5′-VP) is a metabolically-stable phosphate mimic for siRNA and demonstrated that 5′-VP improves the potency of the fully modified siRNAs in vivo. Here, we report an alternative synthesis of 5′-VP modified guide st...
Source: Molecules - January 9, 2019 Category: Chemistry Authors: Mehran Nikan Wenyu Li Garth A. Kinberger Punit P. Seth Eric E. Swayze Thazha P. Prakash Tags: Article Source Type: research

Second to None: Plant Secondary siRNAs as Defensive Agents against Phytophthora.
Abstract The eukaryotic plant pathogen Phytophthora encodes conserved effector proteins to eliminate host secondary siRNAs. In this issue of Cell Host & Microbe, Hou et al. (2019) report that reduction in secondary siRNA levels renders the host hypersusceptible to Phytophthora and plant secondary siRNAs likely serve as trans-species defensive molecules against pathogens. PMID: 30629921 [PubMed - in process]
Source: Cell Host and Microbe - January 9, 2019 Category: Microbiology Authors: Axtell MJ Tags: Cell Host Microbe Source Type: research

Identification of NFAT5 as a transcriptional regulator of the EDN1gene in collecting duct.
Abstract The inner medullary collecting duct (IMCD) produces very high levels of endothelin-1 (ET-1) that acts as an autocrine inhibitor of IMCD Na+and water reabsorption. Recent studies suggest that IMCD ET-1 production is enhanced by extracellular hypertonicity as can occur during high salt intake. While NFAT5 has been implicated in the IMCD ET-1 hypertonicity response, no studies in any cell type have identified NFAT5 as a transcriptional regulator of the EDN1gene; the currently study examined this using a mouse IMCD cell line (IMCD3). Media hypertonicity increased IMCD3 ET-1 mRNA in a dose- and time-dependent ...
Source: Am J Physiol Renal P... - January 9, 2019 Category: Urology & Nephrology Authors: Lakshmipathi J, Wheatley W, Kumar A, Mercenne G, Rodan AR, Kohan DE Tags: Am J Physiol Renal Physiol Source Type: research

POU4F2/Brn-3b transcription factor is associated with survival and drug resistance in human ovarian cancer cells.
In this study, we demonstrate that the Brn-3b transcription factor was increased in different ovarian cancer cells including SKOV3 and A2780 following treatment with cis and pax. Furthermore, sustained increases in Brn-3b were associated with survival in drug resistant cells and correlated with elevated HSP27 expression. In contrast, targeting Brn-3b for reduction using short interfering RNA (siRNA) also resulted in attenuated HSP27 expression. Importantly, blocking Brn-3b expression with siRNA in SKOV3 cells was associated with reduced cell numbers at baseline but also increased cell death after further treatment, indicat...
Source: Oncotarget - January 8, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Correlations of DKK1 with pathogenesis and prognosis of human multiple myeloma.
CONCLUSION: DKK1 is closely related to the pathogenesis and prognosis of human MM, which might be a potential biomarker for the diagnosis of MM. PMID: 30614800 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - January 8, 2019 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research

Modulation on gallbladder carcinoma by TGF- β1 via IGFBP-2.
In conclusion, inhibition of TGF-β1 expression facilitates GC cell apoptosis, inhibits GC cell proliferation, invasion and EMT occurrence. PMID: 30614799 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - January 8, 2019 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research

The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis.
This study aimed to determine Lhx1 interactions and elucidate gene regulatory and physiological roles in the pancreas. Co-immunoprecipitation using β-cell extracts demonstrated an interaction between Lhx1 and Isl1, thus we hypothesized that Lhx1 and Isl1 regulate similar target genes. To test this, we employed siRNA-mediated Lhx1 knockdown in β-cell lines and discovered reduced Glp1R mRNA. Chromatin immunoprecipitation revealed Lhx1 occupancy at a domain also known to be occupied by Isl1 and Ldb1. Through development of a pancreas-wide knockout mouse model ( Lhx1∆Panc), we demonstrate that aged Lhx1∆Panc mice have el...
Source: American Journal of Physiology. Endocrinology and Metabolism - January 8, 2019 Category: Physiology Authors: Bethea M, Liu Y, Wade AK, Mullen R, Gupta R, Gelfanov V, DiMarchi R, Bhatnagar S, Behringer R, Habegger KM, Hunter CS Tags: Am J Physiol Endocrinol Metab Source Type: research

Long non-coding RNA H19 promotes proliferation of Hodgkin's lymphoma via AKT pathway.
CONCLUSIONS: Overexpressed lncRNA H19 promotes HL development by stimulating proliferation of HL cells via AKT pathway. PMID: 30610809 [PubMed - in process]
Source: Journal of B.U.ON. - January 7, 2019 Category: Cancer & Oncology Tags: J BUON Source Type: research

The histone methyltransferase DOT1L is required for proper DNA damage response, DNA repair, and modulates chemotherapy responsiveness.
CONCLUSIONS: In this study, we conclude that DOT1L plays an important role in an early DNA damage response and repair of DNA double-strand breaks via the HR pathway. Moreover, DOT1L inhibition leads to increased sensitivity to chemotherapeutic agents and PARP inhibition, which further highlights its potential clinical utility. Our results further suggest that H3K79me3 can be useful as a predictive and or prognostic marker for rectal cancer patients. PMID: 30616689 [PubMed - in process]
Source: Clinical Colorectal Cancer - January 7, 2019 Category: Cancer & Oncology Authors: Kari V, Raul SK, Henck JM, Kitz J, Kramer F, Kosinsky RL, Übelmesser N, Mansour WY, Eggert J, Spitzner M, Najafova Z, Bastians H, Grade M, Gaedcke J, Wegwitz F, Johnsen SA Tags: Clin Epigenetics Source Type: research

Magnesium lithospermate B protects the endothelium from inflammation-induced dysfunction through activation of Nrf2 pathway.
In this study, we investigated the protective effects of MLB against inflammation-induced endothelial dysfunction in vitro and in vivo, and the underlying mechanisms. Endothelial dysfunction was induced in human dermal microvascular endothelial cells (HMEC-1) in vitro by lipopolysaccharide (LPS, 1 μg/mL). We showed that pretreatment with MLB (10-100 μM) dose-dependently inhibited LPS-induced upregulation of inflammatory cytokines ICAM1, VCAM1, and TNFα, which contributed to reduced leukocytes adhesion and attenuation of endothelial hyperpermeability in HMEC-1 cells. SD rats were injected with LPS (10 mg/kg, ip) to...
Source: Acta Pharmacologica Sinica - January 7, 2019 Category: Drugs & Pharmacology Authors: Gao F, Li JM, Xi C, Li HH, Liu YL, Wang YP, Xuan LJ Tags: Acta Pharmacol Sin Source Type: research

The Cadherin-Catenin Complex is Necessary for Cell Adhesion and Embryogenesis in Nematostella vectensis.
Abstract The cadherin-catenin complex is a conserved, calcium-dependent cell-cell adhesion module that is necessary for normal development and the maintenance of tissue integrity in bilaterian animals. Despite longstanding evidence of a deep ancestry of calcium-dependent cell adhesion in animals, the requirement of the cadherin-catenin complex to coordinate cell-cell adhesion has not been tested directly in a non-bilaterian organism. Here, we provide the first analysis of classical cadherins and catenins in the Starlet Sea Anemone, Nematostella vectensis. Gene expression, protein localization, siRNA-mediated knock...
Source: Developmental Biology - January 7, 2019 Category: Biology Authors: Nathaniel Clarke D, Lowe CJ, James Nelson W Tags: Dev Biol Source Type: research

Cell death mechanisms in a mouse model of retinal degeneration in Spinocerebellar ataxia 7
In this study, we show that in a retinal SCA7 mouse model, polyQ ataxin7 induces stress within the retina and activates Muller cells. Moreover, Unfolded Protein Response and autophagy are activated in SCA7 photoreceptors. We have also shown that the photoreceptor death does not involve a caspase-dependent apoptosis but instead involves apoptosis inducing factor (AIF) and Leukocyte Elastase Inhibitor (LEI/L-DNase II). When these two cell death effectors are downregulated by their siRNA, a significant reduction of photoreceptor death is observed. These results highlight the consequences of polyQ protein expression in the ret...
Source: Neuroscience - January 6, 2019 Category: Neuroscience Source Type: research

Expression of Tripartite Motif-Containing Proteactiin 11 (TRIM11) is Associated with the Progression of Human Prostate Cancer and is Downregulated by MicroRNA-5193.
CONCLUSIONS TRIM11 was upregulated in prostate cancer tissue and was associated with reduced prognosis. TRIM11 expression increased cell proliferation in vitro and was downregulated by miR-5193. PMID: 30608062 [PubMed - in process]
Source: Medical Science Monitor - January 6, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Neuroprotective activity of leukemia inhibitory factor is relayed through myeloid zinc finger-1 in a rat model of stroke
AbstractThe aim of this study was to determine whether leukemia inhibitory factor (LIF) exerts its neuroprotective effects through signal transduction of the transcription factor myeloid zinc finger-1 (MZF-1). According to the hypothesis of this study, MZF-1 mediates LIF-induced neuroprotective signaling during ELVO through increased expression and transcriptional activity. To determine the in vivo role of MZF-1 in LIF-induced neuroprotection, we used Genomatix software was used to MZF-1 sites in the promoter region of the rat superoxide dismutase 3 (SOD3) gene. Stroke was induced via middle cerebral artery occlusion, and ...
Source: Metabolic Brain Disease - January 5, 2019 Category: Neurology Source Type: research

MicroRNA-503-5p Inhibits the CD97-Mediated JAK2/STAT3 Pathway in Metastatic or Paclitaxel-Resistant Ovarian Cancer Cells.
In this study, we investigated whether the miR-503-5p targeting of the CD97 3'-untranslated region (3'-UTR) contributes to ovarian cancer metastasis as well as the underlying mechanism regulating cancer progression. In LPS-stimulated or paclitaxel-resistant ovarian cancer cells, stimulation with recombinant human CD55 (rhCD55) of CD97 in ovarian cancer cells activated NF-κB-dependent miR-503-5p down-regulation and the JAK2/STAT3 pathway, consequently promoting the migratory and invasive capacity. Furthermore, restoration of miR-503-5p by transfection with mimics or NF-κB inhibitor efficiently blocked CD97 expression and ...
Source: Neoplasia - January 5, 2019 Category: Cancer & Oncology Authors: Park GB, Kim D Tags: Neoplasia Source Type: research

Lycium barbarum polysaccharide reduces hyperoxic acute lung injury in mice through Nrf2 pathway
ConclusionsLBP improves hyperoxic ALI via Nrf2-dependent manner. The LBP-induced activation of Nrf2 is mediated, at least in part, by AMPK pathway.
Source: Biomedicine and Pharmacotherapy - January 4, 2019 Category: Drugs & Pharmacology Source Type: research

The protective effect of miR-377 inhibitor against renal ischemia-reperfusion injury through inhibition of inflammation and oxidative stress via a VEGF-dependent mechanism in mice
In conclusion, renal I/R induced miR-377 expression, which upregulated VEGF expression to attenuate renal I/R-induced oxidative stress and inflammation, and finally ameliorated renal dysfunction.
Source: Molecular Immunology - January 4, 2019 Category: Allergy & Immunology Source Type: research

Down-regulation of myocardial infarction associated transcript 1 improves myocardial ischemia-reperfusion injury in aged diabetic rats by inhibition of activation of NF-κB signaling pathway
ConclusionOur study highlights that down-regulation of MIRT1 improves myocardial I/R injury in aged diabetic rats by inhibition of activation of NF-κB signaling pathway.
Source: Chemico Biological Interactions - January 4, 2019 Category: Biochemistry Source Type: research

ASAP ROS-Sensitive Cross-Linked Polyethylenimine for Red-Light-Activated siRNA Therapy
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b18697
Source: ACS Applied Materials and Interfaces - January 4, 2019 Category: Materials Science Authors: Junxia Wang, Xinyu He, Song Shen, Ziyang Cao, Xianzhu Yang Source Type: research

Knockdown of LncRNA GHET1 suppresses prostate cancer cell proliferation by inhibiting HIF ‐1α/Notch‐1 signaling pathway via KLF2
This study aimed to explore the role of LncRNA GHET1 in the pathogenesis of PC. RT ‐qPCR was carried out to examine the relative expression level of GHET1 in PC patients.In vitro, GHET1 siRNA (si ‐GHET1) was used to investigate the biological role of GHET1 in PC cell lines. Cell proliferation was detected by CCK‐8 and colony formation assay, while cell cycle and cell apoptosis were analyzed using flow cytometry. Moreover, western blot was carried out to measure the protein expression lev els of KLF2 and HIF‐1α/Notch‐1 signal pathway. We found that GHET1 showed higher expression in PC tissues and had a negative c...
Source: BioFactors - January 4, 2019 Category: Biochemistry Authors: Yanfeng Zhu, Yue Tong, Jianhua Wu, Yuejuan Liu, Mingjia Zhao Tags: Research Communication Source Type: research

NFAT5 up-regulates expression of the kidney-specific ubiquitin ligase gene Rnf183 under hypertonic conditions in inner-medullary collecting duct cells Signal Transduction
In this study, we elucidated the mechanism of the transcriptional activation of murine Rnf183 in inner-medullary collecting duct cells. Experiments with anti-RNF183 antibody revealed that RNF183 is predominantly expressed in the renal medulla. Among the 37 RNF family members, Rnf183 mRNA expression was specifically increased in hypertonic conditions, a hallmark of the renal medulla. RNF183 up-regulation was consistent with the activation of nuclear factor of activated T cells 5 (NFAT5), a transcription factor essential for adaptation to hypertonic conditions. Accordingly, siRNA-mediated knockdown of NFAT5 down-regulated RN...
Source: Journal of Biological Chemistry - January 4, 2019 Category: Chemistry Authors: Yujiro Maeoka, Yan Wu, Takumi Okamoto, Soshi Kanemoto, Xiao Peng Guo, Atsushi Saito, Rie Asada, Koji Matsuhisa, Takao Masaki, Kazunori Imaizumi, Masayuki Kaneko Tags: Gene Regulation Source Type: research

Knockdown of LncRNA GHET1 suppresses prostate cancer cell proliferation by inhibiting HIF ‐1α/Notch‐1 signaling pathway via KLF2
This study aimed to explore the role of LncRNA GHET1 in the pathogenesis of PC. RT ‐qPCR was carried out to examine the relative expression level of GHET1 in PC patients.In vitro, GHET1 siRNA (si ‐GHET1) was used to investigate the biological role of GHET1 in PC cell lines. Cell proliferation was detected by CCK‐8 and colony formation assay, while cell cycle and cell apoptosis were analyzed using flow cytometry. Moreover, western blot was carried out to measure the protein expression lev els of KLF2 and HIF‐1α/Notch‐1 signal pathway. We found that GHET1 showed higher expression in PC tissues and had a negative c...
Source: BioFactors - January 4, 2019 Category: Biochemistry Authors: Yanfeng Zhu, Yue Tong, Jianhua Wu, Yuejuan Liu, Mingjia Zhao Tags: Research Communication Source Type: research

ZEB1 mediates doxorubicin (Dox) resistance and mesenchymal characteristics of hepatocarcinoma cells.
Abstract The acquired chemoresistance during long term chemotherapy is one of the most important factors to limit the application of Doxorubicin (Dox) on clinical treatment of hepatocellular carcinoma (HCC) patients. Our present study found that Dox resistant HCC (HCC/Dox) cells had greater capability of in vitro migration and invasion compared to their parental cells. HCC/Dox cells exhibited mesenchymal characteristics, which was evidenced by the up regulation of fibronectin, vimentin while down regulation of E-Cadherin. Zeb1, one powerful epithelial mesenchymal transition related transcription factor (EMT-TF), w...
Source: Experimental and Molecular Pathology - January 4, 2019 Category: Pathology Authors: Long L, Xiang H, Liu J, Zhang Z, Sun L Tags: Exp Mol Pathol Source Type: research

Pro-fibrotic effects of IL-17A and elevated IL-17RA in IPF and RA-ILD support a direct role for IL-17A/IL-17RA in human fibrotic interstitial lung disease.
Abstract Interleukin-17 (IL-17) is a T helper 17 (Th17) cytokine implicated in the pathogenesis of many autoimmune diseases, including rheumatoid arthritis (RA). Although IL-17A has a well-established role in murine pulmonary fibrosis models, it's role in the tissue remodeling and fibrosis occurring in idiopathic pulmonary fibrosis (IPF) and RA associated interstitial lung disease (RA-ILD) is not very well defined. To address this question, we utilized complimentary studies to determine responsiveness of human normal and pathogenic lung fibroblasts to IL-17A and used lung biopsies acquired from patients with idiop...
Source: Am J Physiol Lung Ce... - January 3, 2019 Category: Respiratory Medicine Authors: Zhang J, Wang D, Wang L, Wang S, Roden AC, Zhao H, Li X, Prakash YS, Matteson EL, Tschumperlin DJ, Vassallo R Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Lycium barbarum polysaccharide reduces hyperoxic acute lung injury in mice through Nrf2 pathway.
CONCLUSIONS: LBP improves hyperoxic ALI via Nrf2-dependent manner. The LBP-induced activation of Nrf2 is mediated, at least in part, by AMPK pathway. PMID: 30611998 [PubMed - as supplied by publisher]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - January 3, 2019 Category: Drugs & Pharmacology Authors: Zheng G, Ren H, Li H, Li X, Dong T, Xu S, Yan Y, Sun B, Bai J, Li Y Tags: Biomed Pharmacother Source Type: research

Silencing of type II Phosphatidylinositol 4-kinase β stabilizes Prostate apoptosis response-4 and induces apoptosis in cancer cells.
Abstract Type II Phosphatidylinositol 4-kinase β (PtdIns 4-kinase II β) is an enigma among the phosphatidylinositol 4-kinase family. The role of PtdIns 4-kinase II β in MCF-7 cells was addressed with the help of short hairpin RNA (shRNA). PtdIns 4-kinase II β shRNA transfection increased pan caspase activity and induced apoptosis in cancerous MCF-7 cells.  Non-cancerous MCF-10A cells were resistant to PtdIns 4-kinase II β shRNA induced apoptosis. Caspase 8 and 9 inhibitors rescued MCF-7 cells from apoptosis. Shotgun proteomic studies with Flag-tagged PtdIns 4-kinase II β immunoprecipitates showed tumor supp...
Source: The Biochemical Journal - January 3, 2019 Category: Biochemistry Authors: Chaudhry S, Joshi V, Bojjireddy N, Thoh M, Sandur SK, Subrahmanyam G Tags: Biochem J Source Type: research

Down-regulation of myocardial infarction associated transcript 1 improves myocardial ischemia-reperfusion injury in aged diabetic rats by inhibition of activation of NF- κB signaling pathway.
CONCLUSION: Our study highlights that down-regulation of MIRT1 improves myocardial I/R injury in aged diabetic rats by inhibition of activation of NF-κB signaling pathway. PMID: 30611788 [PubMed - as supplied by publisher]
Source: Chemico-Biological Interactions - January 3, 2019 Category: Molecular Biology Authors: Liu Y, Wang T, Zhang M, Chen P, Yu Y Tags: Chem Biol Interact Source Type: research

Potential therapeutic applications of MDA-9/Syntenin-NF- κB-RKIP loop in human liver carcinoma.
CONCLUSION: Our data confirm the key role of MDA-9/Syntenin in HCC biology. The presence of a regulation loop among MDA-9/Syntenin, NF-κB and RKIP provide new pharmacological approaches. PMID: 30608040 [PubMed - as supplied by publisher]
Source: Current Molecular Medicine - January 3, 2019 Category: Molecular Biology Authors: Notarbartolo M, Labbozzetta M, Pojero F, D'Alessandro N, Poma P Tags: Curr Mol Med Source Type: research

Editors' Choice Protein silencing to stop a "silent killer"
Delivery of a single dose of siRNA to the placenta silences proteins and may help prevent preeclampsia.
Source: Science Translational Medicine - January 2, 2019 Category: Biomedical Science Authors: Jay, S. M. Tags: Editors ' Choice Source Type: research