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High-voltage pulsed electric field plus photodynamic therapy kills breast cancer cells by triggering apoptosis.
This study evaluated the effects and mechanism of action of combining irreversible electroporation (IRE) and photodynamic therapy (PDT) in breast cancer cells in vitro and in vivo. Jin's formula was used to assess killing efficacy of different IRE+PDT dosing combinations in breast cancer MCF-7 cells. Flow cytometry, high-content imaging, and confocal laser scanning microscopy were used to detect apoptosis. qRT-PCR and western blotting were used to evaluate expression of apoptosis-related genes and proteins. IRE+PDT combination therapy was administered to BALB/C mice with breast cancer tumors in vivo; tumor size was used to...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The chemokine receptor CCR1 is identified in mast cell-derived exosomes.
Authors: Liang Y, Qiao L, Peng X, Cui Z, Yin Y, Liao H, Jiang M, Li L Abstract Mast cells are important effector cells of the immune system, and mast cell-derived exosomes carrying RNAs play a role in immune regulation. However, the molecular function of mast cell-derived exosomes is currently unknown, and here, we identify differentially expressed genes (DEGs) in mast cells and exosomes. We isolated mast cells derived exosomes through differential centrifugation and screened the DEGs from mast cell-derived exosomes, using the GSE25330 array dataset downloaded from the Gene Expression Omnibus database. Biochemical ...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Alantolactone plays neuroprotective roles in traumatic brain injury in rats via anti-inflammatory, anti-oxidative and anti-apoptosis pathways.
The objective of this study was to evaluate the protective effect of ATL in a rat model of controlled cortical impact (CCI). We observed the neurological scores, brain water content, oxidative stress, neuroinflammation and apoptosis by performing an enzyme-linked immunosorbent assay, western blotting, quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), immunohistochemical (IHC) staining and other methods after CCI. The neurological scores, brain water content, levels of oxidative stress and inflammatory cytokines, and apoptosis index were markedly decreased following the ATL treatment in rats ...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Vitamin D enhances resistance to aspergillus fumigatus in mice via inhibition of excessive autophagy.
In conclusion, Vitamin D deficiency can lead to impaired lung defense in mice, which may be associated with the formation of excessive autophagy-induced lysosomes and increased counts of Treg cells. PMID: 29511432 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A novel liver-targeted nitric oxide donor UDCA-Thr-NO protects against cirrhosis and portal hypertension.
Authors: Sun J, Li M, Fan S, Guo Z, Zhong B, Jin X, Shi W Abstract Portal hypertension (PHT) is a common liver disease that is closely related to cirrhosis and has a high morbidity and mortality. The present study aimed to probe the efficacy of a novel nitric oxide (NO)-releasing agent with NO linked to ursodeoxycholic acid (UDCA) through threonine (UDCA-Thr-NO) as a liver-targeted therapy for cirrhosis and PHT. After intraperitoneal treatment of dimethyl nitrosamine-induced cirrhotic rats for 3 or 4 weeks, UDCA-Thr-NO could prevent ascites formation and reduce portal pressure instead of carotid artery pressure, wh...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Augmenter of liver regeneration (ALR) regulates acute pancreatitis via inhibiting HMGB1/TLR4/NF- κB signaling pathway.
Augmenter of liver regeneration (ALR) regulates acute pancreatitis via inhibiting HMGB1/TLR4/NF-κB signaling pathway. Am J Transl Res. 2018;10(2):402-410 Authors: Pan LF, Yu L, Wang LM, He JT, Sun JL, Wang XB, Wang H, Bai ZH, Feng H, Pei HH Abstract This research aimed to explore the effect of augmenter of liver regeneration (ALR) in acute pancreatitis (AP) of mice and the underlying mechanism. Caerulein were given to mice to get AP models. AP mice were given saline, ALR plasmids or negative control plasmids. Then, pancreas tissues were fixed and stained for histological examination. The levels o...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Zhuangguguanjie formulation protects articular cartilage from degeneration in joint instability-induced murine knee osteoarthritis.
Authors: Lu K, Shi T, Li L, Zhang K, Zhu X, Shen S, Yu F, Teng H, Gao X, Ju H, Wang W, Jiang Q Abstract Zhuangguguanjie formulation (ZG) can provide noticeable relief from joint pain in patients suffering from knee osteoarthritis (OA). However, the underlying mechanism has not been fully described. Male C57BL/6 mice were administered either ZG or normal saline (NS) following surgical destabilization of the medial meniscus (DMM). At weeks 4, 6 and 8 (post-surgery), knee joints were harvested and assessed with Safranin-O staining. Blood serum was collected and tested. In vitro analysis was carried out to evaluate the...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Dihydroartemisinin inhibits indoxyl sulfate (IS)-promoted cell cycle progression in mesangial cells by targeting COX-2/mPGES-1/PGE2 cascade.
Authors: Mungun HK, Li S, Zhang Y, Huang S, Jia Z, Ding G, Zhang A Abstract Dihydroartemisinin (DHA) is a semisynthetic derivative of artemisinin and has been used as an antimalarial drug. Recently, roles of artemisinin and its derivatives in treating diseases besides antimalarial effect were documented. Thus, this study was undertaken to investigate the role of DHA in indoxyl sulfate (IS)-promoted cell cycle progression in glomerular mesangial cells, as well as the potential mechanisms. Under the basal condition, DHA significantly retarded the cell cycle progression as shown by decreased cell percentage in S phase...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Mdivi-1 pretreatment mitigates isoflurane-induced cognitive deficits in developmental rats.
Authors: Gao J, Luo A, Yan J, Fang X, Tang X, Zhao Y, Li S Abstract Accumulating evidence indicates that general anesthetics can cause acute neuroapoptosis and long-term cognitive deficit in models exposed to anesthetics during the brain growth-spurt period. Anesthetics-induced imbalance of mitochondrial fusion and fission preceded and contributed to developmental neuroapoptosis. Accordingly, the imbalance was accompanied by activation of dynamin-related protein (Drp)1 which was closely associated with synaptic degeneration in neurodegenerative diseases. Based on the neuroprotective role of mitochondrial division i...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Multi-parameter evaluation of lumbar intervertebral disc degeneration using quantitative magnetic resonance imaging techniques.
Authors: Xiong X, Zhou Z, Figini M, Shangguan J, Zhang Z, Chen W Abstract Objective: To quantitatively evaluate lumbar disc degeneration with recently-developed quantitative magnetic resonance imaging (MRI) techniques. A series of MRI parameters, including T2*, T1rho relaxation time, apparent diffusion coefficient and gagCEST, were compared and correlated with the Pfirrmann semi-quantitative classification of lumbar intervertebral disc degeneration; the most accurate and relevant MRI parameters of lumbar disc degeneration were identified. Materials and Methods: Thirty-seven subjects (age range, 18-74 years) with no...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Knockout of the placenta specific 8 gene radiosensitizes nasopharyngeal carcinoma cells by activating the PI3K/AKT/GSK3 β pathway.
Knockout of the placenta specific 8 gene radiosensitizes nasopharyngeal carcinoma cells by activating the PI3K/AKT/GSK3β pathway. Am J Transl Res. 2018;10(2):455-464 Authors: Yang R, Tao ZZ, Huang ML, Zheng YF, Dai MY, Zou Y, Chen SM Abstract The present study investigated the radiosensitizing effect of placenta specific 8 (PLAC8) in nasopharyngeal carcinoma (NPC) cells. A PLAC8-knockout CNE2 cell line was constructed using the CRISPR/Cas9 system. The CCK-8 assay demonstrated knockout of PLAC8 significantly reduced cell proliferation and cell survival after irradiation compared to both control cel...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Andrographolide presents therapeutic effect on ulcerative colitis through the inhibition of IL-23/IL-17 axis.
In conclusion, we demonstrated that andrographolide inhibited the activity of IL-23/IL-17 axis and down-stream pro-inflammatory factors so as to suppress inflammation response, resulting in the relieving of UC. PMID: 29511440 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model.
Authors: Yin B, Xue B, Wu Z, Ma J, Wang K Abstract The aim of this study was to explore an innovative method to improve the osteogenic ability of porous titanium. We used gelatin (Gel) and nano-hydroxyapatite (nHA) to construct micro-scaffolds within the pores of porous titanium alloy. We compared three groups: control, Gel:nHA = 1:0, and Gel:nHA = 1:1. We assessed cell attachment, cell proliferation, and osteogenic (alkaline phosphatase [ALP] and collagen type 1 [Col-1]) and cytoskeletal (Talin) gene and protein expression in MC3T3-E1 cells. We also evaluated osteogenic abilities in a rabbit calvarial defect model...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

SRPX2 regulates colon cancer cell metabolism by miR-192/215 via PI3K-Akt.
Authors: Zhao J, Xu J, Zhang R Abstract Colon cancer is one of common cancer in the world and glycolysis is one of the major problems in colon cancer therapy. MicroRNAs (miRNAs) involve in colon cancer progression. Sushi repeat-containing protein X-linked 2 (SRPX2) is associated with poor prognosis in some cancer patients, however, the role of SRPX2 including glycolytic metabolism regulated by miRNAs is unclear in colon cancer. So, the purpose of the present study is to elucidate the underlying mechanism in colon cancer metabolism mediated by SRPX2. Our results revealed that miR-192-5p (miR-192) and miR-215-5p (miR...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Anticancer effect of fufang yiliu yin on human hepatocellular carcinoma SMMC-7721 cells.
Authors: Yang Z, Zhu S, Liu S, Wang X, Han B, Zhang B, Hu X, Yao R, Sun C, Zhu C Abstract Chinese herbal medicine utilizes clinically effective adjuvants that can potentiate the effects of hepatectomy and molecule-targeted drugs for the treatment of hepatocellular carcinoma (HCC). The aim of this study was to investigate the possible molecular mechanisms underlying the antitumor effect of fufang yiliu yin (FYY) on HCC cells. We investigated the effects of FYY on the proliferation, migration, invasion, and apoptosis of SMMC-7721 cells in vitro and in mouse subcutaneous xenograft models in vivo. FYY significantly inh...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Functional peptides for cartilage repair and regeneration.
Authors: Liu Q, Jia Z, Duan L, Xiong J, Wang D, Ding Y Abstract Cartilage repair after degeneration or trauma continues to be a challenge both in the clinic and for scientific research due to the limited regenerative capacity of this tissue. Cartilage tissue engineering, involving a combination of cells, scaffolds, and growth factors, is increasingly used in cartilage regeneration. Due to their ease of synthesis, robustness, tunable size, availability of functional groups, and activity, peptides have emerged as the molecules with the most potential in drug development. A number of peptides have been engineered to r...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Control of macrophage autophagy by miR-384-5p in the development of diabetic encephalopathy.
Authors: Wang B, Huang J, Li J, Zhong Y Abstract The molecular development of diabetic encephalopathy remains ill-defined. Recently, we reported that elimination of inflammatory macrophages alleviated the progress and severity of diabetic encephalopathy. Here, we studied the underlying mechanism. Inflammatory macrophages were isolated from the brain of the mice that received i.p. injection of streptozotocin (STZ) to develop diabetes 6 weeks before, and showed enhanced autophagy activity, seemingly through augmentation of Beclin-1 levels. However, the increases in Beclin-1 levels did not result from enhanced gene tr...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

NudCD1 affects renal cell carcinoma through regulating LIS1/Dynein signaling pathway.
Authors: He H, Dai J, Wang X, Qian X, Zhao J, Wang H, Xu D Abstract Renal cell carcinoma (RCC) is one of the most common malignant tumors in urogenital system with an incidence accounting for about 3% of the whole body malignant tumor. NudC domain containing 1 (NudCD1), a new member of NudC family distributed in nucleus, is found to be upregulated in multiple tumors. However, its expression and role in RCC tissue has not been elucidated. NudCD1 expression in RCC tissue was measured by western blot and immunohistochemistry (IHC). NudCD1 level was elevated by overexpression vector to investigate its regulatory role o...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MiRNA-491-5p inhibits cell proliferation, invasion and migration via targeting JMJD2B and serves as a potential biomarker in gastric cancer.
In this study, we found that miR-491-5p suppressed cell proliferation, invasion and migration by directly targeting the JMJD2B 3'UTR in gastric cancer. Moreover, miR-491-5p was decreased in GC tissues compared with adjacent normal tissues, and JMJD2B had the inverse expression pattern. In contrast to healthy individuals, GC patients had lower miR-491-5p expression in serum (P
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Co-injection of human adipose stromal cells and rhBMP-2/fibrin gel enhances tendon graft osteointegration in a rabbit anterior cruciate ligament-reconstruction model.
The objective of this study was to investigate whether the co-injection of human adipose stromal cells (hASCs) and rhBMP-2/fibrin gel into the bone tunnels of anterior cruciate-ligament reconstructions can effectively enhance tendon graft osteointegration. We performed bilateral reconstructions using autologous semitendinosus tendons in 45 New Zealand rabbits, which we divided into three groups. We injected the bone tunnels with fibrin gel for group 1, rhBMP-2 (1 μg/ml)/fibrin gel for group 2, and hASCs wrapped in rhBMP-2 (1 μg/ml)/fibrin gel for group 3. We sacrificed five rabbits (two for histological assessment an...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

HDAC inhibitors suppressed small cell lung cancer cell growth and enhanced the suppressive effects of receptor-targeting cytotoxins via upregulating somatostatin receptor II.
In conclusion, we found that VPA suppressed SCLC cell growth and increased the expression of SSTR2. These may provide a novel clinical opportunity for enhanced anticancer therapy using the combination strategy of Notch signalling regulator and SSTR2-targeting cytotoxins in SCLC treatments. PMID: 29511449 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Genomic risk variants at 3q22.3 are associated with keloids in a Chinese Han population.
Authors: Lu MZ, Ang QQ, Zhang X, Zhang LF, Yao XH, Lv H, Zheng XD, Lu WS Abstract A keloid is the process of skin healing, collagen synthesis and metabolism of the loss of normal control in a sustained hyperactive state, resulting in excessive proliferation of collagen fibers. A large-scale genome-wide association study (GWAS) has identified multiple single nucleotide polymorphisms (SNPs) in the 3q22.3 loci that are associated with keloids in a Japanese population. However, the associations of SNPs in 3q22.3 with keloids were not confirmed in a selected Chinese population by a replication study. Thus, in the presen...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

lncRNA MALAT1/miR-205-5p axis regulates MPP+-induced cell apoptosis in MN9D cells by directly targeting LRRK2.
Authors: Chen Q, Huang X, Li R Abstract Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), as a long chain non-coding RNA (lncRNA), has been reported to be upregulated in Parkinson's disease (PD). However, the mechanisms underlying this process remain unknown. Hence, to investigate the role of MALAT1 in PD, N-methyl-4-phenylpyridinium (MPP+) was used to induce PD in vitro in the MN9D dopaminergic neuronal cell line and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to induce PD in vivo in C57BL/6 mice. Quantitative Real-Time PCR (qRT-PCR) and western blot assay showed that the expression...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Expression of long non-coding RNA HOXA11-AS is correlated with progression of laryngeal squamous cell carcinoma.
In this study, we firstly investigated the expression of HOXA11-AS in LSCC. Microarray and qRT-PCR showed that the level of HOXA11-AS was significantly higher in LSCC than that in the corresponding adjacent non-neoplastic tissues. ISH revealed that HOXA11-AS was strongly expressed in the nucleus and closely related to the T grade, neck nodal metastasis, and clinical stage. Patients with T3-4 grade, neck nodal metastasis, or advanced clinical stage presented a high HOXA11-AS expression. Kaplan-Meier analysis showed that high HOXA11-AS expression could predict a poor prognosis in LSCC patients. Furthermore, HOXA11-AS knockdo...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Role of Kr üppel-like factor 4 in cigarette smoke-induced pulmonary vascular remodeling.
Role of Krüppel-like factor 4 in cigarette smoke-induced pulmonary vascular remodeling. Am J Transl Res. 2018;10(2):581-591 Authors: Sun D, Li Q, Ding D, Li X, Xie M, Xu Y, Liu X Abstract Pulmonary hypertension (PH) is characterized by excessive proliferation of pulmonary artery smooth muscle cells (PASMCs), leading to dysregulated vascular remodeling. Cigarette smoke (CS) is a common risk factor causing PH, and our previous study showed that CS extract (CSE) stimulated abnormal PASMC proliferation. However, the molecular mechanism remains unclear. In systemic circulation, vascular remodeling in s...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Extensive profiling of circular RNAs and the potential regulatory role of circRNA-000284 in cell proliferation and invasion of cervical cancer via sponging miR-506.
In conclusion, circRNA-000284 promotes cell proliferation and invasion in cervical cancer, and may serve as a promising therapeutic target for cervical cancer patients. Therefore, silence of circRNA-000284 could be a future direction to develop a novel treatment strategy. PMID: 29511454 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Interplay between long noncoding RNA ZEB1-AS1 and miR-101/ZEB1 axis regulates proliferation and migration of colorectal cancer cells.
This study revealed a novel regulatory mechanism between ZEB1-AS1 and miR-101/ZEB1 axis. The interplay between ZEB1-AS1 and miR-101/ZEB1 axis contributed to the proliferation and migration of CRC cells, and targeting this interplay could be a promising strategy for CRC treatment. PMID: 29511455 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-146b-3p regulates the development and progression of cerebral infarction with diabetes through RAF1/P38MAPK/COX-2 signaling pathway.
In this study, we try to explore the relationship between microRNA-146b-3p (miR-146b-3p) and DMCI patients. The peripheral blood mononuclear cells were separated after the patients were selected from our hospital. Firstly, the content of IL-6 and COX-2 was detected by ELISA. Then, the total RNAs were extracted and analyzed by microRNA (miRNA) microarray. Moreover, the target genes of miR-146b-3p were predicted by online miRNA target prediction algorithms. Meanwhile, luciferase reporter system was used for assaying the target gene for miRNA-146b-3p. Simultaneously, RT-PCR assay was used for the miRNA expression detection. F...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Downregulation of FoxM1 inhibits cell growth and migration and invasion in bladder cancer cells.
Authors: Yang X, Shi Y, Yan J, Fan H Abstract The FoxM1 (Forkhead Box M1) transcription factor plays a key role in regulation of cell growth, cell cycle, and transformation. Higher expression of FoxM1 has been observed in various types of human cancers including bladder cancer. However, the exact function of FoxM1 in bladder cancer has not been elucidated. To investigate the cellular and molecular function of FoxM1 in bladder cancer, we measured the consequences of downregulation and upregulation of FoxM1 in bladder cancer cells using MTT assay, wound healing assay, and invasion assay. We found that downregulation ...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

SOX2 inhibits cell proliferation and metastasis, promotes apoptotic by downregulating CCND1 and PARP in gastric cancer.
Authors: Luo J, Yan R, He X, He J Abstract Inconsistent results of Sex-determining region Y-box2 (SOX2) expression have been reported in gastric cancer (GC) before. Our recent studies showed that SOX2 was significantly downregulated in GC cells compared with GES-1 at both mRNA and protein level. Transfected with pcDNA3.1-SOX2 resulted in enforced expression of SOX2 at mRNA and protein levels compared with NC group in undifferentiated cell lines including HGC27 and BGC823. MTT assay showed that exogenous expressed SOX2 suppressed cell proliferation. FC analysis revealed that SOX2-overexpressing cells exhibited cell-...
Source: American Journal of Translational Research - March 9, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Regulatory role of long non-coding RNAs during reproductive disease.
Authors: Liu K, Mao X, Chen Y, Li T, Ton H Abstract Long non-coding RNA (lncRNA) is a group of RNAs with broad biogenesis, which are longer than 200 nt and highly conserved in their secondary and tertiary structures. lncRNA that broadly participates in varied physiological processes in organisms has abundant biological function and can regulate expression of target genes at transcriptional, post-transcriptional and epigenetic levels. LncRNAs can also affect the development of diseases, and therefore be used to diagnose and treat diseases. With new sequencing and microarray techniques, hundreds of lncRNAs involved i...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Polycystic ovary syndrome in mitochondrial disorders due mtDNA or nDNA variants.
CONCLUSIONS: Currently, there is no evidence that PCOS is a phenotypic feature of MIDs. mtDNA variants reported by a single centre in association with PCOS need to be confirmed by multicentre studies. In case of hereditary PCOS, whole exome (genome) sequencing is recommended. PMID: 29422990 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1.
This study was to investigate the interactive roles between IGF-1 and the global DNA methylation/hydroxymethylation, and the status of DNA methylation/hydroxymethylation and associated enzymes such as DNMTs and TETs in peeeclamptic placentas and hypoxic trophoblasts. It was found that IGF-1 was decreased in preeclamptic placentas and hypoxic trophoblasts when compared to the control group using immunohistochemisty, western blot, qRT-PCR and ELISA. Pyrophosphate sequencing showed IGF-1 promoter was significantly hypermethylated in preeclamptic placentas, which was responsible for reduced IGF-1 expression. Preeclamptic place...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Pyrroloquinoline quinone plays an important role in rescuing Bmi-1-/- mice induced developmental disorders of teeth and mandible--anti-oxidant effect of pyrroloquinoline quinone.
This study demonstrated that PQQ played an important role in rescuing mandible osteoporosis and disorder of teeth development in BKO mice by promoting osteoblastic bone formation of mandibular alveolar bone, inhibiting osteoclastic bone resorption, promoting odontoblast cell proliferation of epithelial root sheath area, inhibiting cell apoptosis, scavenging ROS. PMID: 29422992 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Mesenteric injection of adipose-derived mesenchymal stem cells relieves experimentally-induced colitis in rats by regulating Th17/Treg cell balance.
Authors: Fu ZW, Zhang ZY, Ge HY Abstract Efficient delivery routes are critical for the effectiveness of adipose-derived mesenchymal stem cells (ADMSCs) in treating inflammatory bowel disease (IBD). Conventional ADMSC delivery routes include local, intravenous and intraperitoneal injection. Whether mesenteric injection has potential in IBD treatment remains unknown. In the present study, we investigated the therapeutic effects of mesenteric injection of ADMSCs in a trinitrobenzene sulfonic acid-induced rat IBD model and explored whether this treatment affected T helper 17 (Th17)/regulatory T (Treg) cell ratio. The ...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Effect of low-intensity pulsed ultrasound on the biological behaviors of bone marrow mesenchymal stem cells on titanium with different surface topographies.
Authors: An Y, Song Y, Wang Z, Wang J, Wu G, Zhu G, Chen L Abstract The use of low-intensity pulsed ultrasound (LIPUS) is a promising approach to promote osteogenesis. However, few studies have reported the influence of this technique on the osseointegration of endosseous implants, especially regarding different implant topographies. We focused on how the initial interaction between cells and the titanium surface is enhanced by LIPUS and the potential regulatory mechanisms. The bone marrow mesenchymal stem cells (BMSCs) of rats were cultured on two types of titanium surfaces (polished surface, Flat and large grain ...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq.
Authors: Maimaitiaili A, Li J, Aibibula A, Abudureheman M Abstract OBJECTIVE: Uighur medicine Abnormal Savda Munziq (ASMq) shows cardioprotective effect in myocardial ischemia/reperfusion injury (IRI) in animal models, but the molecular mechanism of this effect is not clear. The present study investigates the regulation of nuclear factor kappa B (NF-kappa B) pathway by ASMq in IRI rat models. METHODS: Male Sprague-Dawley rats were divided into three groups: the NF-κB gene knockout group (n = 5); the NF-κB transgenic group (n = 8); and the control group (n = 5). All rats were treated with ASMq for 21...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Melatonin regulates CRE-dependent gene transcription underlying osteoblast proliferation by activating Src and PKA in parallel.
Authors: Tao L, Zhu Y Abstract Several studies have indicated a relationship between melatonin and idiopathic scoliosis, including our previous work which demonstrated that melatonin can inhibit osteoblast proliferation; however, the mechanism remains unclear. Here, we utilized a MTT assay to show that melatonin significantly reduces osteoblast proliferation in a concentration-and time-dependent manner. Through a combination of techniques, including real-time PCR, MTT assays, immunofluorescence, and luciferase assays, we confirmed that melatonin-induced changes in phosphorylated cAMP response element-binding protei...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

HucMSC exosomes-delivered 14-3-3 ζ enhanced autophagy via modulation of ATG16L in preventing cisplatin-induced acute kidney injury.
In this study, we further explored the mechanisms of hucMSC-ex in autophagy for preventing cisplatin-induced nephrotoxicity. We discovered that 14-3-3ζ was contained in hucMSC-ex, and knockdown and overexpression 14-3-3ζ reduced and enhanced the autophagic activity respectively. Furthermore, Knockdown of 14-3-3ζ alleviated the preventive effect of hucMSC-ex. In contrast, overexpression of 14-3-3ζ enhanced the effect. Further results confirmed that hucMSC-ex increased ATG16L expression and that 14-3-3ζ interacted with ATG16L, promoting the localization of ATG16L at autophagosome precursors. In this ...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway.
This study aims to investigate the phytoestrogenic effect of BT and DC extract, and the possible mechanism. The effects on T47D (ER+ cell line) proliferation were evaluated by using MTT assay. The S phase proportion of ER+ cells was determined by using flow cytometry. The estrogen antagonist ICI 182,780 was applied to block the ER function. The activation of ER-mediated PI3K/AKT and ERK signal pathways were observed by using western blot. Expression of ERα and PGR, as well as PS2 and Cyclin D1 were detected by using western blot and real-time quantitative PCR. Firstly, our results found that BT and DC extracts promot...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon.
Authors: Ali BH, Za'abi MA, Karaca T, Suleimani YA, Balushi KAA, Manoj P, Ashique M, Nemmar A Abstract Potassium bromate (KBrO3) is used in many countries in cosmetic and food industries. In this work, we investigated in male Sprague-Dawley rats, the effect of four graded oral doses of KBrO3 (5, 15, 45 and 135 mg/kg/day for 28 days) on renal function tests, inflammation, oxidative damage, and apoptosis, as well as on histopathology, using several traditional and novel renal injury biomarkers in plasma, urine and renal tissues. We also tested the possible ameliorative action of the renoprotective prebiotic agent gum...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Silencing long noncoding RNA PVT1 inhibits tumorigenesis and cisplatin resistance of colorectal cancer.
In this study, we found that PVT1 was highly expressed in CRC tissues and cell lines compared with the corresponding non-cancerous samples and normal colon epithelial cells. Clinically, increased expression of PVT1 was positively correlated with tumor size, advanced histological stages, metastases, poor prognosis, and cisplatin resistance of CRC patients. In vitro studies showed that PVT1 silencing inhibited the proliferation, migration, invasion, and apoptosis escape of CRC cells. Knockdown of PVT1 in cisplatin-resistant CRC cells induced proliferation inhibition and apoptosis, whereas overexpression of PVT1 increased pro...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Hypoxia increases amyloid- β level in exosomes by enhancing the interaction between CD147 and Hook1.
This study was to investigate the role of CD147 in hypoxia-induced accumulation of Aβ in exosomes. Our results showed that hypoxia increased the levels of Aβ40 and Aβ42 in exosomes and enhanced the interaction between CD147 and Hook1 in SH-SY5YAPP695 cells. Moreover, hypoxia increased the interaction between amyloid precursor protein (APP) and CD147 as well as the expression of CD147 in isolated membrane. After we interfered the interaction between CD147 and Hook1 by decreasing Rab22a expression, the hypoxia induced Aβ accumulation in exosomes was significantly suppressed. In addition, the increased int...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

NDUFA4 enhances neuron growth by triggering growth factors and inhibiting neuron apoptosis through Bcl-2 and cytochrome C mediated signaling pathway.
This study aims to investigate effects of NDUFA4 on growth of neurons. LV5-NDUFA4 and LV3-NDUFA4-RNAi lentivirus were constructed and transfected to neurons. Ciclosporin A, together with the two lentivirus were applied to neurons to observe neuron growth, apoptosis, and related protein expression. MTT assay was used to observe neuron growth. Apoptosis was detected by using flow cytometry assay. Real-time PCR was utilized to examine NDUFA4 mRNA expression. Western blot and immunohistochemistry assay were used to detect nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), brain fibroblast growth factor (bFGF)...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Direct conversion of human fibroblasts into functional Leydig-like cells by SF-1, GATA4 and NGFI-B.
Authors: Hou YP, Zhang ZY, Xing XY, Zhou J, Sun J Abstract The reprogramming of fibroblasts to induced pluripotent stem cells raises the possibility that a somatic cell can be reprogrammed to an alternative, differentiated fate without first becoming a stem/progenitor cell. Recent work has shown that fibroblasts can be reprogrammed to other, terminally differentiated cells with a combination of several transcription factors. Here, we report that a combination of four developmental transcription factors; GATA4, SF-1, NGFI-B, and COUP TF2; efficiently reprogrammed human foreskin fibroblasts into functional induced Le...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Herpes zoster chronification to postherpetic neuralgia induces brain activity and grey matter volume change.
CONCLUSIONS: HZ chronification induced functional and structural change in cerebellum, occipital lobe, temporal lobe, parietal lobe and limbic lobe. These changes may be correlated with HZ-PHN chronification. In addition, these changes could be reasons of refractory chronic pain of PHN. PMID: 29423004 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Transcription factor Phf19 positively regulates germinal center reactions that underlies its role in rheumatoid arthritis.
Authors: Ning F, Wang C, Niu S, Xu H, Xia K, Wang N Abstract The Polycomb Repressive Complex 2 (PRC2) component PHD Finger Protein 19 (phf19) gene has been identified to be associated with rheumatoid arthritis (RA) risk. Here we show that Phf19 is highly expressed in murine germinal centers (GCs) and RA patients. To investigate the function of Phf19 in lymphocytes, we generated RAG1-deficient mice reconstituted with Phf19 or control-vector transduced bone marrow (BM) cells. Lymphogenesis in primary lymphoid tissues of Phf19-RM is normal, however, Phf19-RM form enlarged GCs and generate more antibody-secreting cells...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Umbilical cord mesenchymal stem cell transplantation in the treatment of multiple sclerosis.
Authors: Meng M, Liu Y, Wang W, Wei C, Liu F, Du Z, Xie Y, Tang W, Hou Z, Li Q Abstract To investigate the clinical efficacy and safety of umbilical cord mesenchymal stem cell (UCMSC) transplantation for treating multiple sclerosis (MS), the patients with MS were recruited and treated with UCMSC. The procedure of preparing UCMSC was in accordance with the standards formulated by the International Society for Cell Biology. Cell surface markers, multiple differentiation potential and safety of UCMSC for transplantation were detected. The number of cells in each infusion was 1 to 2×106 cells/kg. Patients were re...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

miR-132 suppresses the migration and invasion of lung cancer cells by blocking USP9X-induced epithelial-mesenchymal transition.
This study aimed to investigate the putative role of miR-132 in the metastasis of NSCLC. We determined the function of miR-132 in the migration and invasion of a NSCLC cell line in vitro using a miR-132 inhibitor and mimic. Our results showed overexpression of miR-132 significantly inhibited the migration and invasion of NSCLC cells in vitro. We then identified USP9X as a potential target of miR-132, and demonstrated miR-132 could regulate the expression of USP9X at both the mRNA and protein level. miR-132 could directly bind to the 3' untranslated region (3'-UTR) of USP9X. Inhibition of USP9X by its inhibitor WP1130 reduc...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Generation of an acute retinal photoreceptor degeneration model in rabbits.
Authors: Li K, Liu S, Zhong X, Ge J Abstract PURPOSE: To assess the appropriate dose of sodium nitroprusside for establishing acute retinal photoreceptor degeneration models in rabbits. METHODS: Sodium nitroprusside (SNP) was delivered intravitreously. Sixteen New Zealand White rabbits are divided into four groups randomly: 0.1 mM, 0.25 mM, 0.5 mM SNP intravitreal injection group (experimental groups), and normal saline intravitreal injection group (control group). Assessments included weight, anterior segment photography, fundus photography, Hematoxylin-eosin staining, immunofluorescence, multi-focal electrore...
Source: American Journal of Translational Research - February 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research