Research progress on the role of CaMKII in heart disease.
Authors: Jiang SJ, Wang W Abstract In the heart, Ca2+ participates in electrical activity and myocardial contraction, which is closely related to the generation of action potential and excitation contraction coupling (ECC) and plays an important role in various signal cascades and regulates different physiological processes. In the Ca2+ related physiological activities, CaMKII is a key downstream regulator, involving autophosphorylation and post-translational modification, and plays an important role in the excitation contraction coupling and relaxation events of cardiomyocytes. This paper reviews the relationship ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

COVID-19 associated thromboinflammation of renal capillary: potential mechanisms and treatment.
Authors: Chen X, Yu C, Jing H, Wang C, Zhao X, Zhang J, Zhang S, Liu H, Xie R, Shi J Abstract Coronavirus disease 2019 (COVID-19) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic disease with high morbidity and mortality. Inflammatory and thrombosis are its main manifestations. As an important organ of hemofiltration metabolism, the kidney is prone to blockage and destruction when filter high inflammatory and high viscous blood of COVID-19, resulting in the loss of a large amount of protein, aggravating blood concentration, and then worsening COVID-19 hypercoagul...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Neural mechanisms underlying visual and auditory processing impairments in schizophrenia: insight into the etiology and implications for tailoring preventive and therapeutic interventions.
Authors: Zhuo C, Tian H, Fang T, Li R, Li Y, Kong L, Cai Z, Zheng L, Lin X, Chen C Abstract Schizophrenia is a complex and devastating neuropsychiatric disorder with an unknown etiology. Patients with schizophrenia have a high prevalence of visual disturbances, commonly accompanied by auditory impairments. In recent review articles, the perceptual deficits of visual and auditory sensory processing have been downplayed. However, visual and auditory impairments are associated with hallucinations, which is characteristic of schizophrenia across all cultures. Despite decades of research, the common neural mechanisms un...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

AMPK: implications in osteoarthritis and therapeutic targets.
Authors: Wang J, Li J, Song D, Ni J, Ding M, Huang J, Yan M Abstract Osteoarthritis (OA) is the most common skeletal disease and the leading cause of pain and disability in the aged population (>65 years). However, the underlying factors involved in OA pathogenesis remain elusive which has resulted in failure to identify disease-modifying OA drugs. Altered metabolism has been shown to be a prominent pathological change in OA. As a critical bioenergy sensor, AMP-activated protein kinase (AMPK) mediates not only energy homeostasis but also redox balance in chondrocytes to counter various cell stress. Dysfunction o...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Promising long noncoding RNA DLX6-AS1 in malignant tumors.
Authors: Xue C, Lv L, Jiang J, Li L Abstract Although its diagnosis and treatment have greatly improved in recent decades, cancer remains the major cause of death worldwide. Thus, there is an urgent need to find novel biomarkers and therapeutic targets to improve efficiency of diagnosis and treatment of patients with cancer. Long noncoding RNAs (lncRNAs), a new class of noncoding RNAs (ncRNAs), have been found to play a salient role in human tumorigenesis and progression. Distal-less homeobox 6 antisense RNA 1 (DLX6-AS1) is a novel lncRNA with aberrant expression in various cancers tissues and cell lines compared w...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Immunotherapeutic strategies for sarcoma: current perspectives.
Authors: Li X, Wang G, Cai Z, Sun W Abstract There are more than 100 sarcoma subtypes, each of which is uncommon and challenging to diagnose. Most patients with locally advanced and unresectable sarcomas are still treated with cytotoxic chemotherapy and have low long-term survival. Therefore, novel therapeutic methods are needed to improve the prognosis of patients with sarcomas. Immunotherapy is increasingly recognized as have an essential role in the treatment of malignant tumors. Emerging strategies, such as immune checkpoint inhibitors, vaccines, and adoptive cell therapies have been investigated for the treatm...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Role of azithromycin in antiviral treatment: enhancement of interferon-dependent antiviral pathways and mitigation of inflammation may rely on inhibition of the MAPK cascade?
Authors: Mohanta TK, Arina P, Sharma N, Defilippi P Abstract Azithromycin is a macrolide-type antibiotic used against a broad range of bacterial infection, such as respiratory tract, skin, ear, eye infections, and sexually transmitted diseases. The ongoing severe acute respiratory syndrome (SARS) mediated by Corona Virus 2 (CoVid19) is a global health concern and various countries witnessed the loss of precious human life. In fall 2020, the absence of specific suitable medication or vaccine is still a major cause of concern to fight the pandemic while different countries have already started using their own medicat...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

LncRNA LOC100912373 modulates PDK1 expression by sponging miR-17-5p to promote the proliferation of fibroblast-like synoviocytes in rheumatoid arthritis.
In conclusion, lncRNA LOC100912373 may upregulate the expression of PDK1 by sponging miR-17-5p, accelerating the phosphorylation of AKT and inducing the proliferation of FLSs, thus promoting the occurrence and development of RA. PMID: 33437356 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

GABA-induced motor improvement following acute cerebral infarction.
Authors: Chen Q, Ke J, Cai X, Sun H, Chen Z, Li L, Su M, Fang Q Abstract γ-Aminobutyric acid (GABA) plays a key role in motor learning. In the aftermath of stroke, we monitored GABA+ content of primary motor cortex by magnetic resonance spectroscopy (MRS), assessing its relation to functional motor recovery following a standardized 4-week program of rehabilitation. The cohort included 20 patients, each experiencing stroke within 2 weeks of symptom onset. Twenty age-matched healthy subjects were also recruited as controls. GABA+ levels were determined at baseline and following rehabilitation, performed only on...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Endothelial cell CD36 deficiency prevents normal angiogenesis and vascular repair.
Authors: Bou Khzam L, Son NH, Mullick AE, Abumrad NA, Goldberg IJ Abstract Endothelial cells (ECs) maintain vascular integrity and mediate vascular repair and angiogenesis, by which new blood vessels are formed from pre-existing blood vessels. Hyperglycemia has been shown to increase EC angiogenic potential. However, few studies have investigated effects of fatty acids (FAs) on EC angiogenesis. Cluster of differentiation 36 (CD36) is a FA transporter expressed by ECs, but its role in EC proliferation, migration, and angiogenesis is unknown. We sought to determine if circulating FAs regulate angiogenic function in a...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Ahypoxia-related signature enhances the prediction of the prognosis in hepatocellular carcinoma patients and correlates with sorafenib treatment response.
In conclusion, a risk signature developed with the expression of 10 hypoxia associated genes improved the prognosis prediction of HCC and correlated with sorafenib treatment response. PMID: 33437359 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Expression profile of ACTL8, CTCFL, OIP5 and XAGE3 in glioma and their prognostic significance: a retrospective clinical study.
Conclusions: The results suggest that the cohort of glioma does express ACTL8, CTCFL, OIP5 and XAGE3 at both mRNA and protein levels indicating glioma is CTAs-rich tumors. CTCFL protein and the combined ACTL8 and/or CTCFL protein might act as poor prognostic markers for glioma and as potential ideal combined antigens for glioma immunotherapy. PMID: 33437360 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Engulfment and cell motility 1 promotes tumor progression via the modulation of tumor cell survival in gastric cancer.
CONCLUSIONS: Our results indicate that ELMO1 promotes tumor progression by modulating tumor cell survival in human GC. PMID: 33437361 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Polydopamine coating with static magnetic field promotes the osteogenic differentiation of human bone-derived mesenchymal stem cells on three-dimensional printed porous titanium scaffolds by upregulation of the BMP-Smads signaling pathway.
Authors: Kong L, Han Y, Lu Q, Zhou D, Wang B, Wang D, Zhang W, Xiang H, Li M, Wang F Abstract Bone regeneration has always been a hot topic for orthopedic surgeons. The role of polydopamine coating in promoting bone regeneration has attracted much attention. Static magnetic field (SMF) is considered an effective and noninvasive treatment for enhancing bone regeneration. However, the effect of polydopamine combined with SMF on bone regeneration on scaffolds is not clear. The aim of this study was to investigate the effects and potential mechanism of polydopamine coating combined with SMF on bone regeneration in thre...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Correlation of Hypoxia-inducible facto-1 α and C-reactive protein with disease evaluation in patients with ulcerative colitis.
Correlation of Hypoxia-inducible facto-1α and C-reactive protein with disease evaluation in patients with ulcerative colitis. Am J Transl Res. 2020;12(12):7826-7835 Authors: Yu S, Li B, Hao J, Shi X, Ge J, Xu H Abstract OBJECTIVE: To investigate the expression levels of hypoxia-inducible factor-1α (HIF-1α) and C-reactive protein (CRP) in patients with ulcerative colitis and correlations of HIF-1α and CRP levels with disease severity. METHODS: A total of 82 patients with confirmed ulcerative colitis were enrolled in this study and according to the disease severity grading, th...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Enzastaurin enhances ATRA-induced differentiation of acute myeloid leukemia cells.
Authors: Li ZY, Liang C, Ding M, Weng XQ, Sheng Y, Wu J, Lu H, Cai X Abstract All-trans retinoic acid (ATRA) is considered to be the sole clinically-useful differentiating agent in the treatment of acute myeloid leukemia (AML). However, ATRA has been effective only in acute promyelocytic leukemia (APL) but not other subtypes of AML. Therefore, discovering strategies to sensitize cells to ATRA may lead to the development of ATRA-based treatments in non-APL AML patients. In the present study, a clinically-achievable concentration of enzastaurin enhanced ATRA-induced differentiation in AML cell lines, HL-60 and U937 a...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Circ_USP36/miR-182-5p/KLF5 axis regulates the ox-LDL-induced injury in human umbilical vein smooth muscle cells.
Authors: Zhao Q, Lu YH, Wang X, Zhang XJ Abstract Atherogenesis is a chronic inflammatory process, closely related to high morbidity and mortality. Circular RNAs (circRNAs) were reported to function in atherosclerosis. However, the functional impact of circRNA ubiquitin-specific Protease 36 (circ_USP36) on atherosclerosis and the possible mechanism are still unclear. Serum specimens were collected from atherosclerosis patients and healthy volunteers. Human umbilical vein smooth muscle cells (HUVSMCs) exposed with 25 μg/mL oxidized low-density lipoprotein (ox-LDL) were utilized to simulate atherosclerosis. Expres...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

SCAMP3 is regulated by miR-128-3p and promotes the metastasis of hepatocellular carcinoma cells through EGFR-MAPK p38 signaling pathway.
CONCLUSION: SCAMP3 can be controlled by miR-128-3p and can mediate the EGFR-MAPK p38 signaling pathway to inhibit HCC cell metastasis, which is expected to become a promising therapeutic target for HCC. PMID: 33437366 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

CLOCK disruption aggravates carotid artery stenosis through endoplasmic reticulum stress-induced endothelial-mesenchymal transition.
In conclusion, the disruption of CLOCK function aggravates EndMT via the IRE1α-XBP1 axis, contributing to carotid artery stenosis. PMID: 33437367 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-335 targets the MEK/ERK pathway to regulate the proliferation and metastasis of colon cancer.
Authors: Yang C, Wang M, Zhou J, Chi Q Abstract Several studies have proved the tumor-suppressive effects of miR-335 but its role in colon cancer via regulation of the Raf/MEK/ERK signalling pathway is yet unknown. As such the main motive of conducting the present study was to elucidate the role of miR-335 in colon cancer via regulation of Raf/MEK/ERK signalling pathway and to explore its therapeutic potential. The results revealed significant (P
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

iTRAQ quantitative proteomic analysis differentially expressed proteins and signal pathways in henoch-sch önlein purpura nephritis.
In this study, iTRAQ quantitative proteomic analysis was performed on renal tissues collected from patients with HSPN and compared with those of patients after nephrectomy (controls). A total of 149 differentially expressed proteins (DEPs) were detected, of which, 97 being upregulated and 52 down-regulated. Protein functions and classifications were analyzed using Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, protein domains. expressive hierarchical clustering analysis and protein-protein interaction (PPI) analysis were also conducted for DEPs. The results of bioinformatics analysis in...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

CYP2J2 promotes the development of hepatocellular carcinoma by increasing the EETs production to improve HIF-1 α stability.
CONCLUSION: The up-regulated levels of CYP2J2 and 14, 15-EET in HCC cells improved the stability of HIF-1α thourgh inhibiting PHD expression, which further promoted the malignant development of HCC. PMID: 33437370 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Reactivation of oncogenes involved in G1/S transcription and apoptosis pathways by low dose decitabine promotes HT29 human colon cancer cell growth in vitro.
CONCLUSIONS: We concluded that low-dose DAC treatment resulted in a cancer-promoting effect in HT29 cell lines. Mechanistically, high methylation levels at the promoter region of oncogenes with dominant effects in CRC, such as BCL2 in HT29, might play a role in suppressing CRC by inhibiting oncogene expression. Low-dose DAC treatment triggered BCL2 expression by decreasing its promoter methylation level, thereby resulting in cancer promotion. PMID: 33437371 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

INSR mediated by transcription factor KLF4 and DNA methylation ameliorates osteoarthritis progression via inactivation of JAK2/STAT3 signaling pathway.
CONCLUSION: Our findings suggested that INSR, as a key regulator for OA, was regulated by transcription factor KLF4 and DNA methylation, thereby mediating the activation of JAK2/STAT3 signaling, which was considered as an underlying therapeutic target for OA. PMID: 33437372 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Long-noncoding RNA Peg13 alleviates epilepsy progression in mice via the miR-490-3p/Psmd11 axis to inactivate the Wnt/ β-catenin pathway.
In conclusion, this paper illuminated the function and upstream mechanism of Psmd11 in epilepsy. Psmd11 was upregulated by Peg13 at a miR-490-3p dependent way, thus inactivating the Wnt/β-catenin pathway and alleviating epilepsy course in mice, which may be a promising approach for epilepsy treatment. PMID: 33437373 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

In vitro investigation of protective mechanisms of triptolide against coronary heart disease by regulating miR-24-3p-BCL2L11 axis and PPARs-PGC1 α pathway.
This study intended to explore its molecular regulation mechanism in CHD. It is shown in this study that TTL contributed to the proliferation and migration of in vitro cell models of CHD (endothelial cells) and the inhibition of apoptosis, and had an improvement effect on apoptosis factors and endoplasmic reticulum stress (ERS). From its mechanisms, TTL evidently downregulates miR-24-3p which is elevated in CHD, and evidently upregulates BCL2-like 11 (BCL2L11) which is suppressed in CHD, as well as affects the activation of peroxisome proliferator-activated receptors (PPARs)-Peroxisome proliferator activated receptor-&gamm...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Decreased expression of adenosine receptor 2B confers cardiac protection against ischemia via restoring autophagic flux.
Authors: Zhan E, Cao W, Fan X, Zhang R, Du H, Xu Y, Li L, Dong N, Li S Abstract Adora2B (adenosine receptor 2B) has been reported as one of the key modulators during cardiac remodeling after acute myocardial infarction (AMI). However, the molecular mechanism involved has not been well investigated. Thus, our study aims to investigate whether Adora2B contributes to cardiac remodeling after AMI and its underlying mechanisms. Adenovirus harboring Adora2B or shAdora2B was injected in the border zone in a mouse model of AMI experimentally produced by permanent ligation of left anterior descending (LAD) coronary artery. ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Kaempferol attenuated cisplatin-induced cardiac injury via inhibiting STING/NF- κB-mediated inflammation.
In conclusion, these results showed that KPF had great potential in attenuating cisplatin-induced cardiac injury. Besides, greater emphasis should be placed in the future on natural active compounds containing KPF with anti-inflammatory effects for the treatment of these diseases. PMID: 33437376 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Pediculoplasty combined with vertebroplasty for the treatment of Kummell's disease without neurological impairment: robot-assisted and fluoroscopy-guided.
Authors: Wang B, Wang Y, Zhao Q, Zhao H, Zhang Y, Zhan Y, Yan L, Hao D Abstract With the development of radiology and minimally invasive technology, vertebroplasty has become the mainstream treatment for Kummell's disease. However, the catastrophic complication of bone cement displacement appears occasionally. We use robot-assisted pediculoplasty combined with vertebroplasty to avoid such complications. From January 2015 to January 2018, 87 patients suffering from thoracolumbar Kummell's disease without neurological symptoms were treated by robot-assisted pediculoplasty combined with vertebroplasty. Pediculoplasty ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Preservation of level IIb lymph nodes during supraomohyoid neck dissection for clinically node-negative oral squamous cell carcinoma.
CONCLUSION: Patients with clinically N0 OSCC have a low rate of level IIb lymph node metastasis. Level IIb lymph nodes resection are not necessary during SOHND, which thereby protects the accessory nerve and its branches from damage and improves patient quality of life. PMID: 33437378 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

L51P, a novel mutation in the PAS domain of hERG channel, confers long QT syndrome by impairing channel activation.
Authors: Wang M, Gao M, Fang S, Zheng R, Peng D, Luo Q, Yu B Abstract The human ether-à-go-go-related gene (hERG) potassium channel mediates the repolarization of ventricular action potentials. Mutations in the KCNH2 cause long QT syndrome (LQTS) and are associated with cardiac arrhythmias and sudden death. Here, we functionally analyzed a mutation of hERG potassium channel (p.L51P), gaining novel insights into clinical genotype-phenotype relationships. Potassium currents were recorded by whole-cell patch clamping in HEK293 cells transiently transfected with wild-type and/or mutant hERG potassium channel. Im...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

A novel transverse talar tunnel achieved less vessel damage and better drilling safety for ATFL reconstruction: a cadaveric study with three-dimensional microCT.
CONCLUSION: The TT achieves a superior graft bending angle and intraosseous blood supply protection than the AMMT, DMMT, and PMMT, and is less likely to result in cartilage damage than the TNT. The 20-mm blind-ended tunnels achieve less vessel damage and better drilling safety than transosseous tunnels. PMID: 33437380 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

A digital method of measuring cartilage defects under an arthroscope.
This study aimed to validate the accuracy and utility of this method. In this controlled laboratory study, the ACG method was validated by measuring the sizes of three cartilage defects in a knee joint of a pig, using the following techniques: traditional arthroscopic measurement by ruler (TAR), ACG, incised measurement by computer graphics (ICG), and incised measurement by ruler (IR, control, gold standard). Measurements were conducted by two blinded trained observers. Intra- and inter-observer variabilities were determined by calculating the intra-class correlation coefficient (ICC). Consistency among TAR, ACG, ICG and I...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Reduced hydration-induced decreased caveolin-1 expression causes epithelial-to-mesenchymal transition.
Authors: Yang S, Zhao J, Huang S, Shu B, Yang R, Chen L, Xu Y, Xie J, Liu X, Jia J, Qi S Abstract The reduced hydration environment induced by disruption of epithelial barrier function after injury results in excessive scarring, but the underlying mechanisms are poorly understood. We demonstrated that exposing keratinocytes to a reduced hydration environment causes epithelial-to-mesenchymal transition (EMT) and induces caveolin-1-dependent downregulation of E-cadherin. Reduced caveolin-1 expression and increased Snail expression are associated with low expression levels of E-cadherin. Caveolin-1 downregulation incr...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

The inhibition of BRAF activity sensitizes chemoresistant human ovarian cancer cells to paclitaxel-induced cytotoxicity and tumor growth inhibition.
Authors: Zhao L, Huang L, Zhang J, Fan J, He F, Zhao X, Wang H, Liu Q, Shi D, Ni N, Wagstaff W, Pakvasa M, Fu K, Tucker AB, Chen C, Reid RR, Haydon RC, Luu HH, Shen L, Qi H, He TC Abstract Ovarian cancer is one of the most common cancers in women and the second most common cause of gynecologic cancer death in women worldwide. While ovarian cancer is highly heterogeneous in histological subtypes and molecular genetic makeup, epithelial ovarian cancer is the most common subtype. The clinical outcomes of ovarian cancer largely depend on early detection and access to appropriate surgery and systemic therapy. While comb...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Shikonin promotes osteogenesis and suppresses osteoclastogenesis in vitro.
Authors: Zhou L, Wang J, Zhao J, Kuai F, Yang H Abstract Shikonin, as a traditional Chinese herbal medicine with a role of anti-cancer, anti-inflammatory, anti-bacterial and other effects. However, there are few studies on the effect of shikonin on osteoporosis. Therefore, the purpose of this study aims to investigate the role and mechanism of shikonin on differentiation of BMSCs and BMMs into osteoblasts and osteoclasts formation. In our study, we treated the cells with different concentrations of shikonin, and then illuminated its effect on oteogenesis and osteoclast differentiation by ALP/alizarin red staining, ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Surgery-induced downregulation of hippocampal sirtuin-1 contributes to cognitive dysfunction by inhibiting autophagy and activating apoptosis in aged mice.
CONCLUSION: These findings suggest that surgery-induced downregulation of hippocampal SIRT1 participates in cognitive impairment after surgery by inhibiting the autophagy process and activating apoptosis. PMID: 33437385 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Analysis of mutations in six Chinese families with autosomal dominant polycystic kidney disease.
Authors: Wang H, Dai S, Zhang J, Li Y, Gan Y, Lu T, Zhu Y, Wu J, Lin N, Tang F, Luo J Abstract Autosomal dominant polycystic kidney disease (ADPKD) is the common hereditary kidney disease, resulting from mutations in polycystic kidney disease 1 (PKD1) and polycystic kidney disease 2 (PKD2). Clinical data and genetic features of six Chinese families including ADPKD patients were analyzed via Next generation sequencing (NGS), Sanger sequencing, and multiplex ligation-dependent probe amplification. In family A, the proband (II5) with polycystic kidney (PK), hypertension, left ventricular hypertrophy, and valvular hear...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

m6A RNA methylation regulators contribute to malignant development and have a clinical prognostic effect on cervical cancer.
In conclusion, m6A RNA methylation regulator is a key player in the malignant progression of cervical cancer and has potential role in the stratification of prognosis and the formulation of treatment strategies. PMID: 33437387 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.
In this study naïve-state mES were derived and maintained in the presence of a selective protein kinase C pathway inhibitor (PKCi; Gӧ6983) to study the function of Mbd3 during mES apoptosis. Mbd3 overexpression in mES decreased the total cell number and viability, and it also dramatically increased the rate of apoptosis. Further investigation of Mbd3 overexpression revealed a 3-fold increase in the proapoptotic/prosurvival protein ratio (Bax/Bcl-2) and elevated RNA expression levels of apoptosis-related genes, including Bim, Trail, Fasl, and caspase 3, with reduced Bcl-2 RNA expression levels. Removal of PKCi from th...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

SOX12 expression is associated with progression and poor prognosis in human breast cancer.
Authors: Xu J, Zhang J, Li L, Mao J, You T, Li Y Abstract The sex-determining region Y-box 12 (SOX12) is implicated in several oncogenic signaling pathways of multiple types of cancer; however, the biological effects of SOX12 on breast cancer has yet to be elucidated. Here, we assessed SOX12 expression using real-time quantitative PCR in 142 pairs of breast cancer and adjacent normal tissues (ANTs) and immunohistochemistry in 524 breast cancer and 147 ANTs. The effects of SOX12 on breast cancer progression, clinicopathological variables, and prognostic value were then investigated. SOX12 expression was markedly ele...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Sevoflurane-induced neuronal apoptosis in neonatal mice is prevented with intranasal administration of insulin.
This study provides experimental evidence for a potential effective, yet simple, method to prevent anesthesia-induced neurotoxicity in children, especially in infants. PMID: 33437390 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

A novel homozygous mutation in the meiotic gene MSH4 leading to male infertility due to non-obstructive azoospermia.
Authors: Tang D, Xu C, Geng H, Gao Y, Cheng H, Ni X, He X, Cao Y Abstract Non-obstructive azoospermia (NOA) is the most severe form of male infertility. Although some causes have been established, including genetic causes, the etiology in most cases remains idiopathic. Mutations in MSH4 (OMIM: 602105), an important gene involved in meiosis, may be related to female infertility due to primary ovarian insufficiency (POI) and male NOA. Here, we report a novel homozygous stop-gain mutation of MSH4 associated with NOA. Whole exome sequencing (WES) and bioinformatic analysis were performed in a patient with NOA from a co...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

A disease progression prediction model and nervous system symptoms in coronavirus disease 2019 patients.
This study aimed to investigate factors affecting coronavirus disease 2019 (COVID-19) progression, also to explore the clinical features and prognosis of nervous system symptom (NSS) involved COVID-19 patients. 417 COVID-19 patients were analyzed in this retrospective study, and they were clinically classified as severe patients and non-severe patients. According to NSS involved status, COVID-19 patients were further divided into NSS patients and non-NSS patients. Elderly cases, males, common comorbidities, NSS, respiratory/cardiovascular/gastrointestinal symptoms, bilateral lesion, multifocal lesion, bacterial infection, ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

The vitamin D status and serum eosinophilic cationic protein levels in infants with cow's milk protein allergy.
Authors: Dogan E, Sevinc E Abstract Eosinophil cationic protein (ECP) is a cytotoxic protein released from eosinophils. The level of ECP increases in some allergic diseases. Recently, vitamin D deficiency has been suggested to be a risk factor for childhood allergic disease. The first aim of the study is to measure the serum vitamin D levels and ECP in infants with cow's milk protein allergy (CMPA) and compare them with controls. The second aim of this study is to investigate whether vitamin D levels are correlated with ECP or not. Sixty-two infants with CMPA were compared to 58 healthy, similar to distribution of ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Influence of variants of the drosha, mir499a, and mir938 genes on susceptibility to acute lymphoblastic leukemia in an admixed population from the brazilian amazon.
Authors: de Souza TP, de Carvalho DC, Wanderley AV, Fernandes SM, Rodrigues JCG, Cohen-Paes A, Fernandes MR, Mello Junior FAR, Pastana LF, Vinagre LWMS, Silva ALDC, de Assumpção PP, Santos S, Khayat AS, Dos Santos NPC Abstract Acute Lymphoblastic Leukemia (ALL) is the most common type of cancer in children. Polymorphisms that alter the normal function of the microRNAs involved in the development of ALL have been widely investigated, although published data on these polymorphisms in admixed populations are scarce. We investigated the role of 10 polymorphisms in the microRNA and protein-coding genes of ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Long non-coding RNA AFAP1-AS1 promotes cell growth and inhibits apoptosis by binding to specific proteins in germinal center B-cell-like diffuse large B-cell lymphoma.
In this study, we explored its biological functions and molecular mechanisms in the progression of GCB-DLBCL. We discovered, via bioinformatics, that patients with a high expression of AFAP1-AS1 had significantly poor disease-free survival (DFS) and overall survival (OS). Subsequent assays demonstrated that AFAP1-AS1 knockdown inhibited cell proliferation and prompted arrest of the G0/G1 cell cycle and apoptosis in GCB-DLBCL cell lines. Proteomics analysis indicated that hundreds of proteins were deregulated after AFAP1-AS1 knockdown and KEGG pathway analysis revealed that the deregulated proteins belonged to multiple sign...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Brequinar inhibits enterovirus replication by targeting biosynthesis pathway of pyrimidines.
Authors: Fu H, Zhang Z, Dai Y, Liu S, Fu E Abstract Infection of human enteroviruses could cause diverse diseases ranging from mild respiratory symptoms to neurological complications, and even death. Currently, no-FDA approved antiviral drug is available for clinical treatment of human enteroviruses infection. Brequinar is an immunosuppressive drug currently being used for the prevention of organ graft rejection. The drug repurposing studies show that Brequinar exhibits potent antiviral activity against diverse viruses, including flaviviruses, alphavirus, rhabdovirus, and influenza viruses. The antiviral effect of ...
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Erratum: Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.
Authors: Jiang W, Zheng B, Zhang XH, Yue LY, Liu C, Ma D, Yang Z, Wen JK Abstract [This corrects the article on p. 4778 in vol. 8, PMID: 27904679.]. PMID: 33437397 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research

Erratum: Insulin treatment enhances pseudomonas aeruginosa biofilm formation by increasing intracellular cyclic di-GMP levels, leading to chronic wound infection and delayed wound healing.
Authors: Wei Q, Zhang Z, Luo J, Kong J, Ding Y, Chen Y, Wang K Abstract [This corrects the article on p. 3261 in vol. 11, PMID: 31312343.]. PMID: 33437398 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 14, 2021 Category: Research Tags: Am J Transl Res Source Type: research