ITGB3/CD61: a hub modulator and target in the tumor microenvironment.
Authors: Zhu C, Kong Z, Wang B, Cheng W, Wu A, Meng X Abstract β3 integrin (ITGB3), also known as CD61 or GP3A, is one of the most widely studied components in the integrin family. As an adhesion receptor on the cell surface, ITGB3 participates in reprogramming tumor metabolism, shaping the stromal and immune microenvironment, facilitating epithelial to mesenchymal transition (EMT) and endothelial to mesenchymal transition (End-MT) and maintaining tumor stemness, etc. Recent studies proposed various intervention strategies against ITGB3 and have achieved promising outcomes in several types of tumor. Here, we r...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Principles of digital PCR and its applications in current obstetrical and gynecological diseases.
Authors: Mao X, Liu C, Tong H, Chen Y, Liu K Abstract Digital PCR (dPCR) is a revolutionary technique to precisely quantify nucleic acids. For its high sensitivity and specificity, this technique has been widely replicated worldwide. To verify its applicability, we reviewed all the related articles in PubMed database published before May 10, 2019. Original articles and reviews on the topics were selected. Entered key words included "digital PCR/dPCR", "advantage", "combined use", "microfluidic chip", "gynecological cancer/tumor". We found that dPCR has shown great p...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Prognostic value of circulating lymphocyte B and plasma immunoglobulin M on septic shock and sepsis: a systematic review and meta-analysis.
Authors: Tian L, Zhu J, Jin J, Tong C, Zeng W, Deng S, Zou S Abstract OBJECTIVE: Normal B lymphocyte function and antibody secretion during inflammation can provide critical protection for the host. We aimed to synthesize existing evidence to explore whether circulating B cells and plasma immunoglobulin M (IgM) levels were associated with survival during sepsis. METHODS: PubMed, Embase, ISI Web of Knowledge, Cochrane Central Register of Controlled Trials were systematically searched. Studies with data on circulating B cells and plasma IgM levels within the initial 24 hours after sepsis onset were selected. ...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Integrated assessment of CDK1 upregulation in thyroid cancer.
Authors: Zheng HP, Huang ZG, He RQ, Lu HP, Dang YW, Lin P, Wen DY, Qin YY, Luo B, Li XJ, Mo WJ, Yang H, He Y, Chen G Abstract Cyclin-dependent kinase 1 (CDK1) has a unique role in cell cycle regulation, as it is crucial for cell cycle progression and cell division. The aim of the present study was to use a combination of various detection methods to examine the expression and clinical significance of CDK1 in thyroid cancer (THCA). We used in-house tissue microarrays, immunohistochemistry, public RNA-sequencing, gene microarrays, and meta-analyses to conduct a comprehensive analysis of the role of CDK1 in the occurr...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

BEZ235 enhances chemosensitivity of paclitaxel in hepatocellular carcinoma through inhibiting the PI3K/Akt/mTOR pathway.
Authors: Liu X, Xie C, Li A, Zhang Y, Liu X, Zhou S, Shen J, Huo Z, Cao W, Ma Y, Xu R, Xing Y, Xie Y, Cai S, Tang X Abstract Desensitization of hepatocellular carcinoma (HCC) to paclitaxel chemotherapy is a major deterrent to successful treatment of the cancer. Abnormal activation of the PI3K/Akt/mTOR, pathway is a common outcome of chemotherapy for HCC. Therefore, we investigated whether BEZ235, a dual PI3K and mTOR inhibitor, could increase the sensitivity of HCC to paclitaxel. In vitro results showed that paclitaxel, combined with BEZ235, inhibited HCC cell proliferation and migration, arrested the cell cycle in...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MiRNA-130a-3p inhibits cell proliferation, migration, and TMZ resistance in glioblastoma by targeting Sp1.
In this study, we observed that the expression of Sp1 was upregulated while that of microRNA (miR)-130a-3p was downregulated in glioblastoma cell lines. Sp1 was validated as a target of miR-130a-3p; increased levels of miR-130a-3p inhibited the proliferation, migration, and temozolomide (TMZ) resistance of the glioblastoma cells. However, Sp1 overexpression compromised the inhibitory effects of miR-130a-3p. Our study elucidates the functional interaction between miR-130a-3p and Sp1 in the development and progression of glioblastoma, suggesting a potential therapeutic target for the disease. PMID: 31934277 [PubMed] (Sou...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

1H-NMR metabolomics identifies significant changes in hypermetabolism after glutamine administration in burned rats.
In this study, 1H nuclear magnetic resonance spectroscopy (1H-NMR) was used to examine the effects of glutamine on plasma metabolites in burned rats and to explore the underlying mechanisms. After burn injury, the rats exhibited significant increases in resting energy expenditure (REE) and hypercatabolism, and anabolism was inhibited. The levels of metabolites that reflect the proteolysis of skeletal muscle, such as alanine, histidine, leucine, valine, 3-methylhistidine and creatine, were significantly increased. In addition, the burned rats exhibited energy synthesis dysfunction, as evidenced by a decrease in the ATP conc...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Albiflorin alleviates ovalbumin (OVA)-induced pulmonary inflammation in asthmatic mice.
Authors: Cai Z, Liu J, Bian H, Cai J Abstract In the present study, the effects of albiflorin (ALB) on the pulmonary inflammation induced by ovalbumin (OVA) in an asthmatic mouse model were investigated. Airway hyperreactivity (AHR) in asthmatic mice was detected using the acetylcholine stimulation test. Eosinophilia cells in the serum of asthmatic mice were counted. Hematoxylin and eosin (H&E) staining was used to observe pathological changes in lung tissue. Inflammatory cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were detected in bronchoalveolar lavage fluid (B...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Differentiation induction of human breast cancer cells by arsenite in combination with tetrandrine.
CONCLUSION: The Erk signaling pathway may be crucially involved in the differentiation induction of breast cancer cells in vitro and in vivo. Collectively, our results suggest that the combination can probably serve as promising candidates for the development of novel therapeutic approaches for different types of breast cancer. PMID: 31934280 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Transplanted mouse liver stem cells at different stages of differentiation ameliorate concanavalin A-induced acute liver injury by modulating Tregs and Th17 cells in mice.
Authors: Li S, Bi Y, Wang Q, Xu M, Ma Z, Yang Y, Chang Y, Chen S, Liu D, Duan Z, Hong F, Chen Y Abstract Acute liver failure (ALF) is a disease with a considerably high mortality rate that still lacks a safe and effective treatment. Transplantation of liver stem cells (LSCs) has been considered to be a promising therapeutic alternative for ALF since LSCs have been shown to be involved in immunomodulation and functional reconstruction of the liver. Our present study evaluated and compared the protective effects of the two mouse LSC lines, YE and R5, as well as those of adult mouse hepatocyte (HC), on concanavalin A ...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Regulation of cartilage damage caused by lack of Klotho with thioredoxin/peroxiredoxin (Trx/Prx) system and succedent NLRP3 activation in osteoarthritis mice.
Authors: Gu Y, Ren K, Jiang C, Wang L, Yao Q Abstract This present study aims to verify the underlying mechanism that anti-aging protein Klotho protects cartilages against the damage induced by oxidative stress. The Klotho expression level in the articular cartilages of mice with osteoarthritis (OA) was measured by using western blotting and quantitative real-time PCR. This work also investigated the effects of Klotho on chondrocyte functions, such as PI3K/Akt pathway, apoptosis and reactive oxygen species (ROS) production, through overexpressing Klotho in chondrocytes by transfecting with the plasmid encoding Klot...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-940 inhibits epithelial-mesenchymal transition of glioma cells via targeting ZEB2.
Authors: Xu R, Zhou F, Yu T, Xu G, Zhang J, Wang Y, Zhao L, Liu N Abstract MicroRNAs have been found ectopically expressed in many cancers and play essential roles in tumor EMT progress. Recent studies identified decreased miR-940 expression in glioma cells and may serve as a tumor-suppressor. However, whether miR-940 involve in glioma EMT remain poorly understood. Here we confirmed that miR-940 was significantly reduced in glioma cells and tissues. Introduction of miR-940 dramatically suppressed invasion and migration of glioma cells. Gain-of-function experiments showed ZEB2 as a direct target of miR-940, knockdow...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-205 inhibits the apoptosis of renal tubular epithelial cells via the PTEN/Akt pathway in renal ischemia-reperfusion injury.
This article aims to investigate whether microRNA-205 (miR-205) is involved in this process and to determine its role in the hypoxia-induced injury of renal tubular epithelial cells (TECs). We found that miR-205 was significantly downregulated in rats with renal IRI and in HK-2 cells with hypoxia-reoxygenation injury (HRI) in vitro. In vitro, overexpression of intracellular miR-205 by transfection of a miR-205 mimic significantly reduced apoptosis, and this antiapoptotic effect was antagonized by a miR-205 inhibitor. Moreover, we confirmed that PTEN is a target of miR-205. miR-205 exerted its protective effect by inhibitin...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Tumor-suppressor microRNA-151-5p regulates the growth, migration and invasion of human breast cancer cells by inhibiting SCOS5.
This study was designed to investigate the role and therapeutic implications of miR-151-5p in breast cancer. The results of the present study revealed that miR-151-5p is significantly downregulated in breast cancer tissues and cell lines. Overexpression of miR-151-5p in SK-BR-3 and CAMA-1 cells inhibits their proliferation and colony formation. Wound heal and transwell assays showed that miR-151-5p inhibits the migration and invasion of the SK-BR-3 and CAMA-1 breast cancer cells. TargetScan analysis followed by the dual luciferase assay confirmed SOCS5 to be the target of miR-151-5p in breast cancer. The expression of SOCS...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Decreased expression of microRNA-510 in intestinal tissue contributes to post-infectious irritable bowel syndrome via targeting PRDX1.
CONCLUSION: MiR-510 downregulation in intestinal tissue might contribute to PI-IBS via targeting PRDX1. The results of this study will not only enrich the pathogenesis of PI-IBS but also make us understand the biological activity of miR-510 and provide important experimental basis for PI-IBS clinical treatment targeting miR-510. PMID: 31934286 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

BPTF cooperates with p50 NF- κB to promote COX-2 expression and tumor cell growth in lung cancer.
In this study, the COX-2 promoter-binding protein BPTF (bromodomain PHD finger transcription factor) was detected, identified and verified by biotin-streptavidin-agarose pulldown, mass spectrum analysis and chromatin immunoprecipitation (ChIP) in lung cancer cells, respectively. The expressions of COX-2 and BPTF in lung cancer cell lines, mouse tumor tissues and human clinical samples were detected by RT-PCR, Western blot and immunohistochemistry assays. The interaction of BPTF with NF-kB was analyzed by immunoprecipitation and confocal immunofluorescence assays. We discovered and identified BPTF as a new COX-2 promoter-bi...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effects of irisin on the differentiation and browning of human visceral white adipocytes.
CONCLUSIONS: Our data suggest that (1) irisin may increase mitochondrial respiration and glycolysis in visceral adipocytes by a UCP-1 independent pathway; (2) irisin promotes anti-inflammatory activity on fat tissue. PMID: 31934288 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Anti-GPC3 single-chain scFv antibody acts as an agent for radio-immunoimaging in diagnosing hepatocellular carcinoma.
In conclusion, the established anti-GPC3 scFv antibody had the potential to become an agent for radio-immunoimaging in diagnosing HCC and act as a targeted antibody for further radio-immunotherapy of HCC. PMID: 31934289 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Streptococcus may aggravate inflammatory damage in chronic nephritis via the chemotaxis of Th22 cells.
CONCLUSION: Streptococcus may cause the chemotaxis of Th22 cells to kidney tissue, leading to or aggravating nephritis injury. PMID: 31934290 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

LncRNA ANRIL promotes angiogenesis and thrombosis by modulating microRNA-99a and microRNA-449a in the autophagy pathway.
In conclusion, ANRIL promotes angiogenesis and thrombosis by upregulating the expression of miR-99a and miR-449a during autophagy. PMID: 31934291 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Bone-strengthening supplement (BSP) promotes bone and cartilage repair, for the treatment of Osteonecrosis of Femoral Head: an MRI-based study.
Authors: Li Z, Wei J, Xiao H, Zhu L, Weng X, Jin J, Zhang J, Wang H, Ge J, Tao T, Pei L, Dong X, Wang L Abstract Currently, no effective drug treatment is available for bone and joint disease, a disorder of the bone and cartilage cells. Osteonecrosis of the femoral head (ONFH) is an example of bone and joint disease. It is progressive, with femoral head collapse resulting from the death of osteocytes and the bone marrow, leading to a poor quality of life and surgical interventions. However, the mechanism of this disease is still unknown, and the effects of current therapy are not satisfactory. In our previous study...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Comparison of treatment efficacy and safety between drug-eluting bead transarterial chemoembolization with CalliSpheres ® microspheres and conventional transarterial chemoembolization as first-line treatment in hepatocellular carcinoma patients.
Comparison of treatment efficacy and safety between drug-eluting bead transarterial chemoembolization with CalliSpheres® microspheres and conventional transarterial chemoembolization as first-line treatment in hepatocellular carcinoma patients. Am J Transl Res. 2019;11(12):7456-7470 Authors: Ma Y, Zhao C, Zhao H, Li H, Chen C, Xiang H, Zheng C, Ma C, Luo C, Qiu H, Yao Y, Hu H, Xiong B, Zhou J, Zhu H, Long Q Abstract We aimed to compare the treatment response, survivals and safety of drug-eluting bead (DEB) transarterial chemoembolization (TACE) with CalliSpheres® microspheres (CSM) and conventi...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Evaluation of PD-1 blockade using a multicellular tumor spheroid model.
Authors: Zhou S, Zhu M, Meng F, Shao J, Xu Q, Wei J, Liu B Abstract Three-dimensional multicellular tumor spheroids are invaluable due to their ability to recapitulate major physiological characteristics of solid tumors. The lack of appropriate in vitro models to evaluate the effects of PD-1 blockade has restricted use of PD-1 inhibitors. Here, we established spheroids from a human gastric cell line to evaluate the cytotoxicity of the PD-1 blockade. Mature spheroids were formed 72 h after seeding tumor cells into ultra-low attachment microplates. We induced PD-L1 expression of spheroids using IFN-γ and TNF-&a...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Combined treatment with cysteamine and leukemia inhibitory factor promotes guinea pig oocyte meiosis in vitro.
This study provides an important scientific basis for further efforts toward guinea pig in vitro fertilization, cloning, and gene editing by establishing an animal model for human reproduction and related diseases. PMID: 31934295 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Analysis of prognostic factors and clinical outcomes in uterine cervical carcinoma with isolated para-aortic lymph node recurrence.
Authors: Chen CS, Ou YC, Lin H, Wang CJ, Chen HC, Fang FM, Hsu HC, Huang YJ, Wang YM, Huang CC, Chou SY, Cheng JY, Huang EY Abstract To evaluate clinical outcomes and to identify prognostic factors in isolated para-aortic lymph node (PALN) recurrence, we retrospectively reviewed 65 patients who developed PALN recurrence as the first site of tumor progression from a total of 1521 patients who were treated with curative pelvic radiation therapy (RT) for uterine cervical carcinoma between May 1993 and January 2017. Forty-five of the 65 patients received salvage therapy. The median salvage PALN radiation dose was 54 Gy...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Identifying TF-miRNA-mRNA regulatory modules in nitidine chloride treated HCC xenograft of nude mice.
Authors: Gao L, Xiong DD, He RQ, Lai ZF, Liu LM, Huang ZG, Yang X, Wu HY, Yang LH, Ma J, Li SH, Lin P, Yang H, Luo DZ, Chen G, Dang YW Abstract Nitidine chloride (NC) has reported tumor suppressive activities for various human cancers, including hepatocellular carcinoma (HCC). Nevertheless, the pharmacological mechanism of NC on HCC has not previously been elucidated. SMMC7721 HCC cell lines, before and after the treatment of NC, were injected into nude mice for a subcutaneous tumor xenograft model. MiRNA and mRNA sequencing were performed for both control and treated xenograft tissues to further analyze differenti...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Visual deprivation modifies glutamate receptor expression in visual and auditory centers.
In this study, we showed that the expression patterns of five glutamate receptor (GluR) subunits which involved in regulating the synaptic plasticity in mouse primary visual (V1) cortex and primary auditory (A1) cortex undergone elaborate modification with layer-specificity after visual deprivation using dark-exposure (DE). The expression levels of NR1 and NR2B were increased, and those of GluR1 and NR2B in the V1 cortex were decreased after DE. In the A1 cortex, the expression levels of NR1, NR2A and NR2B were increased, and the expression levels of GluR1 and GluR2 were decreased after DE. The altered expression levels of...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Exploration and validation of downregulated microRNA-199a-3p, downstream messenger RNA targets and transcriptional regulation in osteosarcoma.
This article aimed to investigate the potential regulatory targets of microRNA-199a-3p (miR-199a-3p) in OS and to contribute to the understanding of miR-199a-3p-related OS regulatory mechanisms. MicroRNA-related Gene Expression Omnibus (GEO) chips, ArrayExpress chips and literature data were used to determine the expression of miR-199a-3p in OS and pooled to explore its potential clinical value. To investigate the target genes of miR-199a-3p further, we integrated the results from the following three-part gene study: Twelve online prediction tools were used to predict the target genes of miR-199a-3p; the GEO GSE89370 chip ...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas.
Authors: Yang M, Yang X, Wang S, Xu L, Ke S, Ding X, Sun W, Gao J Abstract The goal of this study was to assess the role of high-mobility group box 1 (HMGB1)-induced endothelial cell (EC) pyroptosis in systemic inflammatory response syndrome (SIRS) following radiofrequency (RF) ablation of hepatic hemangiomas. We enrolled 76 patients with hepatic hemangioma who underwent RF ablation. Serum concentrations of HMGB1, interleukin (IL)-1β, IL-18 and lactate dehydrogenase (LDH) were determined at different time points. Immunohistochemistry staining (IHC) was performed to evaluate the expressions of HMGB1, NLRP3, cas...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-26b-3p/ANTXR1 signaling modulates proliferation, migration, and apoptosis of glioma.
Authors: Geng F, Lu GF, Ji MH, Kong DY, Wang SY, Tian H, Xie ZM, Pan M, Gong NL Abstract Glioma is a common malignant human brain tumor. The progression of glioma is associated with dysregulation of various microRNAs. Previous studies have demonstrated that microRNA-26b-3p (miR-26b-3p) is correlated with the pathogenesis of various tumors, but the functional role of miR-26b-3p and its underlying mechanisms in glioma are not clear. Here, we found that overexpression of miR-26b-3p repressed cell migration and proliferation and promoted apoptosis. In contrast, the opposite effects were observed when miR-26b-3p was inh...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Paeoniflorin exhibits antitumor effects in nasopharyngeal carcinoma cells through downregulation of NEDD4.
Authors: Chen Y, Zhang R, Zhao W, Lv M, Chen M, Yan Y, Feng S Abstract Paeoniflorin (PF), which is isolated from the paeony root, possesses tumor suppressive function in a variety of malignancies. However, it is unknown whether PF possesses anticancer activity in nasopharyngeal carcinoma (NPC) and which molecular mechanism is involved in PF-triggered antitumor function. In the current study, we measured cell proliferation, apoptotic death, cell cycle, cell motility in NPC cells after PF exposure. We found that PF suppressed cell proliferation, stimulated apoptosis, triggered cell cycle arrest, and impeded cell migr...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation.
This study simulated the pathological process associated with AD and proposed that hypoxia of intravascular cells with normal blood oxygen saturation might be one of a pathogenic mechanisms of AD. Therefore, this work may provide a new theoretical basis for AD prevention and treatment. PMID: 31934303 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effects of HER2 on the invasion and migration of gastric cancer.
This study aimed to propose a new therapeutic target by exploring the regulating pathway of HER2. METHODS: Reverse transcription polymerase chain reaction (RT-PCR), western blot and immunohistochemistry staining were used respectively to detect the expression of HER2, Twist, E-cadherin and Fascin1 in both HER2 knockdown and overexpressed cell lines. Trans-well chamber assay and wound healing assay were used to detect the invasive ability of gastric cancer cells. The correlation between HER2 and Twist was analyzed based on specimens obtained from 118 patients with gastric cancer. RESULTS: HER2 silencing decreased th...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Probiotics alleviate cognitive dysfunction associated with neuroinflammation in cardiac surgery.
In this study, we revealed that probiotics alleviate cognitive dysfunction associated with neuroinflammation in cardiac surgery. Rats were administered a probiotic or placebo once a day by gavage for 2 weeks until the day of surgery. Cardiac surgery was induced by ischemia/reperfusion of the left coronary artery. Key factors, such as the gut microbiome, the gut barrier and the blood-brain barrier (BBB), were systematically investigated to determine whether changes in the gut microbiome lead to neuroinflammation. We used 16S rDNA sequencing to confirm that cardiac surgery induced intestinal flora dysbiosis by altering the n...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Human gingiva tissue-derived MSC ameliorates immune-mediated bone marrow failure of aplastic anemia via suppression of Th1 and Th17 cells and enhancement of CD4+Foxp3+ regulatory T cells differentiation.
Authors: Zhao J, Chen J, Huang F, Wang J, Su W, Zhou J, Qi Q, Cao F, Sun B, Liu Z, Bellanti JA, Zheng S Abstract Accumulating evidence has revealed that human gingiva-derived mesenchymal stem cells (GMSCs) are emerging as a new line of mesenchymal stem cells and may have the potential to control or even treat autoimmune diseases through maintaining the balance between Th and Treg cells. Given that GMSCs have a robust immune regulatory function and regenerative ability, we investigated the effect of GMSCs on preventing T cell-mediated bone marrow failure (BMF) in a mouse model. We observed that GMSCs markedly improv...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

A combination of low-dose decitabine and chidamide resulted in synergistic effects on the proliferation and apoptosis of human myeloid leukemia cell lines.
In this study, the combination of DAC at low doses and chidamide showed enhanced inhibition of myeloid leukemia cell (K562, THP-1) growth. As a novel HDACi, chidamide increased the level of ace-H3K18 expression. Combined use of low-dose DAC and chidamide arrested the cell cycle at the G0/G1 phase by upregulating p21 expression, and the combination also suppressed PI3K/AKT/mTOR signaling pathway. Furthermore, chidamide enhanced the apoptotic effect of DAC by downregulating expression of Bcl-2 and pro-caspase-3 and upregulating that of Bax, cleaved PARP-1, and caspase-9. Moreover, the mitochondrial transmembrane potential wa...
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Erratum: Significant association of TREM-1 with HMGB1, TLRs and RAGE in the pathogenesis of insulin resistance in obese diabetic populations.
Authors: Subramanian S, Pallati PK, Sharma P, Agrawal DK, Nandipati KC Abstract [This corrects the article on p. 3224 in vol. 9, PMID: 28804542.]. PMID: 31934308 [PubMed - in process] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 16, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The oncogenic potential of small nuclear ribonucleoprotein polypeptide G: a comprehensive and perspective view.
Authors: Mabonga L, Kappo AP Abstract Small nuclear ribonucleoprotein polypeptide G (SNRPG), often referred to as Smith protein G (SmG), is an indispensable component in the biogenesis of spliceosomal uridyl-rich small nuclear ribonucleoprotein particles (U snRNPs; U1, U2, U4 and U5), which are precursors of both the major and minor spliceosome. SNRPG has attracted significant attention because of its implicated roles in tumorigenesis and tumor development. Suggestive evidence of its varying expression levels has been reported in different types of cancers, which include breast cancer, lung cancer, prostate cancer ...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Mechanisms supporting potential use of bone marrow-derived mesenchymal stem cells in psychocardiology.
Authors: Liu J, Zhang L, Liu M Abstract Despite great efforts made in recent years, globally cardiovascular disease (CVD) remains the most common and devastating disease. Pharmacological, interventional and surgical treatments have proved to be only partly satisfactory for the majority of patients. A major underlying cause of poor prognosis is a high comorbidity rate between CVD and mental illness, which calls for the approaches of psychocardiology. As psychiatric disorders and CVD can influence each other bidirectionally, it is necessary to develop novel therapies targeting both systems simultaneously. Therefore, ...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

MiR-145: a potential biomarker of cancer migration and invasion.
Authors: Xu WX, Liu Z, Deng F, Wang DD, Li XW, Tian T, Zhang J, Tang JH Abstract MircoRNAs (miRNAs) are a diverse family of highly-conserved small non-coding RNAs, which range from approximately 18 to 25 nucleotides in size. They regulate gene expression transcriptionally or post-transcriptionally via binding to the 3'-untranslated region (3'-UTR) of target message RNAs (mRNAs). MiRNAs have emerged as molecular regulators that participate in physiological and pathological processes of diverse malignancies. Among them, miRNA-145 (miR-145) played a profound role in tumorigenesis and progression of various neoplasms. ...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Clinical roles of miR-136-5p and its target metadherin in thyroid carcinoma.
Authors: Gao RZ, Que Q, Lin P, Pang YY, Wu HY, Li XJ, Chen G, He Y, Yang H Abstract BACKGROUND: Thyroid carcinoma (TC) is a common malignancy of the endocrine system. This research aimed to examine the expression levels of miR-136-5p and metadherin (MTDH) in TC and unveil their potential targeting relationship. METHODS: TC microRNA (miRNA) microarray and miRNA-sequencing data were collected to evaluated miR-136-5p expression. We assessed the comprehensive expression of miR-136-5p by calculating the standard mean difference (SMD) and summary receiver operating characteristic curves (sROC). Subsequently, the miR-...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Intra-articular injection of etoricoxib-loaded PLGA-PEG-PLGA triblock copolymeric nanoparticles attenuates osteoarthritis progression.
In conclusion, our study confirmed the chondro-protective role of etoricoxib in OA, and proved the curative effects of etoricoxib-loaded PLGA-PEG-PLGA NPs in vivo. PMID: 31814887 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Unraveling the action mechanism of polygonum cuspidatum by a network pharmacology approach.
Authors: Pan B, Shi X, Ding T, Liu L Abstract As a popular Chinese herbal medicine (CHM), polygonum cuspidatum is widely used to treat various diseases in China. However, its biological function and action mechanism have yet to be systematically explored. In the present study, we first identified 14 potential active ingredients of polygonum cuspidatum using the TCMSP server and then conducted an in silico target prediction for these ingredients using PharmMapper. The subsequent KEGG pathway enrichment analysis of the 57 identified potential targets revealed that they were closely associated with cancer and gynecolo...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Identification of potential core genes in metastatic renal cell carcinoma using bioinformatics analysis.
Authors: Li C, Shao T, Bao G, Gao Z, Zhang Y, Ding H, Zhang W, Liu F, Guo C Abstract Most cases of mRCC without an early finding are not candidates for curative therapies, which may be one of the reasons for the poor patient prognosis. Therefore, candidate markers to diagnose the disease and treatment with high efficiency are urgently demanded. Three datasets of mRNA microarray have been assessed to discover DEGs between tissues from metastatic RCC and RCC. 111 DEGs in total were identified according to the expression profile result of genes in the database of GEO. Enrichment analyses for GO and KEGG have been cond...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

LncRNA HOXA11-AS accumulation-induced microRNA-761 downregulation regulates cell growth by targeting TRIM29 in papillary thyroid cancer.
In this study, we reported for the first that miR-761 expression was down-regulated in PTC tissues and cell lines, and its decrease was associated with tumor size and TNM stage. Gain- and loss-of function experiments revealed that miR-761 inhibited cell proliferation, colony formation and cell cycle progression in vitro and in vivo. Moreover, TRIM29 was identified as a direct downstream target of miR-761 in PTC cells and mediated the functional effects of miR-761 in PTC. Restoration of TRIM29 expression at least partially abolished the biological effects of miR-761 on PTC cells. Furthermore, overexpression of lncRNA HOXA11...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Hypoxia-induced circular RNA has_circRNA_403658 promotes bladder cancer cell growth through activation of LDHA.
In this study, our aim was to investigate the role of circular RNA on the hypoxic adaptive response in bladder cancer. Here, we identified a hypoxia-inducible circular RNA, has-circRNA-403658 that contributes to bladder cancer progression. Has-circRNA-403658 is spliced from its host gene, ZNF292, through back-splicing between the 1st and 4th exon. We demonstrated that has-circRNA-403658 was an important circRNA that upregulated in bladder cancer cells under hypoxia, and higher has-circRNA-403658 levels were associated with poorer survival outcome. Silencing has-circRNA-403658 in bladder cancer cells inhibited cell growth a...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Anatomical research on strength of screw track fixation in novel cortical bone trajectory for osteoporosis lumbar spine.
CONCLUSION: This study showed that the novel trajectory could be fixed more closely with cortical bone compared to traditional cortical bone trajectory technique, and thus it may reduce the surgical exposure to the elders and help them to recover quickly after the operation. PMID: 31814892 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

The TGF β1-FOXM1-HMGA1-TGFβ1 positive feedback loop increases the cisplatin resistance of non-small cell lung cancer by inducing G6PD expression.
In this study, we found that glucose-6-phosphate dehydrogenase (G6PD), a critical enzyme of the pentose phosphate pathway, contributed to cisplatin resistance in NSCLC. The experimental results showed that transforming growth factor beta 1 (TGFβ1) increased the expression of G6PD by activating the forkhead box protein M1-high mobility group AT-hook 1-G6PD (FOXM1-HMGA1-G6PD) transcriptional regulatory pathway, in which TGFβ1 inhibited the ubiquitination and degradation of FOXM1 protein. Additionally, HMGA1 induced TGFβ1 expression, and neutralized TGFβ1 in the culture medium downregulated HMGA1 levels, s...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Cisplatin induces calcium ion accumulation and hearing loss by causing functional alterations in calcium channels and exocytosis.
Authors: Lu J, Wang W, Liu H, Liu H, Wu H Abstract In recent years, molecular biology and biochemistry have been a focus of studies on the ototoxic side effects of cisplatin. In this paper, the application of cisplatin for 4 h and 72 h was studied from the perspective of electrophysiological function. Patch clamp experiments and immunofluorescence staining were performed on inner hair cells of the cochlea. The patch-clamp results showed that the calcium current amplitude decreased significantly at 4 h and 72 h after cisplatin treatment, the reversal potential was negatively polarized, and the activation time decrea...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Cordycepin inhibits human ovarian cancer by inducing autophagy and apoptosis through Dickkopf-related protein 1/ β-catenin signaling.
In this study, we treated human ovarian cancer cells with different doses of cordycepin and found that it dose-dependently reduced ovarian cancer cell viability, based on Cell counting kit-8 reagent. Immunoblotting showed that cordycepin increased Dickkopf-related protein 1 (Dkk1) levels and inhibited β-catenin signaling. Atg7 knockdown in ovarian cancer cells significantly inhibited cordycepin-induced apoptosis, whereas β-catenin overexpression abolished the effects of cordycepin on cell death and proliferation. Furthermore, we found that Dkk1 overexpression by transfection downregulated the expression of c-Myc ...
Source: American Journal of Translational Research - December 10, 2019 Category: Research Tags: Am J Transl Res Source Type: research