Roles of hyperuricemia in metabolic syndrome and cardiac-kidney-vascular system diseases.
Authors: Wang H, Zhang H, Sun L, Guo W Abstract Uric acid is the final product of purine metabolism. Hyperuricemia is defined as a condition where the level of uric acid exceeds the normal range. The most well-known disease induced by hyperuricemia is gout. However, many studies have reported that hyperuricemia also plays important roles in cardiac-kidney-vascular system diseases and metabolic syndrome. Although hyperuricemia has been known for a long time, its pathophysiology remains poorly understood. In this review, we highlight studies on advanced pathological mechanisms for injuries induced by hyperuricemia, s...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Opioid receptor mu 1 (OPRM1) A118G polymorphism (rs1799971) and postoperative nausea and vomiting.
CONCLUSIONS: OPRM1-A118G polymorphism (A> G) is associated with a reduced risk of postoperative vomiting, but not nausea, pruritus and dizziness. The results should be interpreted with caution due to limited sample and possible heterogeneity between the included studies. Well-designed and large-scale studies are necessary to confirm our results. PMID: 30323865 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Role of double knockdown of tPA and MMP-9 on regulating the left ventricular function and remodeling followed by transverse aortic constriction-induced hypertrophic cardiomyopathy in mice.
This study tested the hypothesis that extracellular matrix accumulation in tPA-/-/MMP-9-/- [double-knockout (DKO)] may be protective against left ventricular (LV) remodeling and dysfunction following transverse aortic constriction (TAC)-induced hypertrophic cardiomyopathy in mice. Wild-type C57BL/6 mice (n = 20) were equally categorized into sham-control (SC1) and TAC1. Similarly, DKO mice (n = 20) were equally divided into two groups (i.e., SC2 and ATC2). By days 28/60 after TAC, LV ejection fraction (LVEF) was significantly higher in TAC2 than TAC1, whereas LV end-systolic/diastolic dimensions displayed an opposite patte...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Low-dose nicotine reduces the homing ability of murine BMSCs during fracture healing.
This study examined the effects of exposure to low-dose nicotine at sub-toxic concentrations on the proliferation, differentiation and migration of bone marrow stem cells (BMSCs) in vitro and their homing to fracture site in C57BL/6 mice. BMSCs were investigated in cells treated with or without nicotine (1 μM to 1 mM). Different concentrations of nicotine exhibited varied effects on BMSCs growth regulation and bone differentiation. CCK8 test significantly increased at a high nicotine concentration of 1 mM while calcium nodule staining with Alizarin red decreased at the same concentration. In vitro scratch test, Transwel...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Eplerenone attenuates myocardial infarction in diabetic rats via modulation of the PI3K-Akt pathway and phosphorylation of GSK-3 β.
Eplerenone attenuates myocardial infarction in diabetic rats via modulation of the PI3K-Akt pathway and phosphorylation of GSK-3β. Am J Transl Res. 2018;10(9):2810-2821 Authors: Mahajan UB, Chandrayan G, Patil CR, Arya DS, Suchal K, Agrawal Y, Ojha S, Goyal SN Abstract We investigated the effect of eplerenone on myocardial infarcted diabetic rats via modulation of the PI3K/Akt pathway and its downstream target GSK-3β. Diabetes was induced by administration of a single dose of streptozotocin (55 mg/kg IP). Diabetic rats received either eplerenone or PI3k/Akt antagonist (wortmannin) or in combi...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Transcribed ultraconserved noncoding RNA uc.160 acts as a negative regulator in gastric cancer.
CONCLUSION: uc.160 is significantly down-regulated in gastric carcinomas and can inhibit the tumor growth both in vitro and in vivo, suggesting that uc.160 may be used as a diagnostic marker and therapeutic target of gastric malignancies. PMID: 30323869 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-129-5p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting DNMT3A.
CONCLUSION: Taken together, our results demonstrate that miR-129-5p plays a significant role in glioma suppression through inhibition of DNMT3A, which may provide a novel therapeutic strategy for treatment of glioma and other DNMT3A-driven cancers. PMID: 30323870 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Differentially circulating exosomal microRNAs expression profiling in oral lichen planus.
In conclusion, circulating exosomal miR-34a-5p could be a potential biomarker for evaluating the severity of OLP. PMID: 30323871 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Mouse dendritic cell migration in abdominal lymph nodes by intraperitoneal administration.
This study makes a foundation for future researches of DC-based immunotherapy as a treatment modality against cancer. PMID: 30323872 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Effects of human bone morphogenetic protein 2 (hBMP2) on tertiary dentin formation.
Authors: Ni SL, Zhang J, Liu X, Li XW, Sun YJ, Zhang X, Wang L, Lu JJ, Cui Y, Zheng CY, Han B, Sun HC Abstract Formation of tertiary dentin to maintain pulp vitality is a major odontoblastic response to dental pulp injury. Human bone morphogenetic protein 2 (hBMP2) can promote proliferation and differentiation of odontoblasts. Current study is interested in evaluating if the hBMP2 can promote the regeneration of tertiary dentin and cure dental pulp injury using the adenoviral vector to deliver hBMP2 cDNA into the pulp. Primary culture of dental pulp cells of exfoliated deciduous teeth (hDPCs) was established. Human...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Microglial activation: an important process in the onset of epilepsy.
This study aimed to investigate the effects of microglial activation on the onset of epilepsy. Microglias cultured in vitro were stimulated with different concentrations of coriaria lactone (CL), and the effects on cell cycle and apoptosis were examined using flow cytometry. Then microglia were stimulated with 5×10-5 mol/L CL, and levels of cyclin D1 and interleukin-1β (IL-1β) mRNA were measured by fluorescence quantitative PCR; tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in supernatant were detected by radioimmunoassay. Finally, microglia-conditioned medium (MCM) obtain...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

FoxO4 promotes myocardial ischemia-reperfusion injury: the role of oxidative stress-induced apoptosis.
In conclusion, the FoxO4/ROS pathway represents a potentially novel mechanism underlying apoptosis during myocardial I/R injury. Therapeutic strategies targeting FoxO4 might represent new treatments for myocardial I/R injury. PMID: 30323875 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Low-intensity pulsed ultrasound inhibits RANKL-induced osteoclast formation via modulating ERK-c-Fos-NFATc1 signaling cascades.
Authors: Meng J, Hong J, Zhao C, Zhou C, Hu B, Yang Y, Jiang G, Li S, Shi Z, Cai X, Yan S Abstract Low-intensity pulsed ultrasound (LIPUS), which is a noninvasive form of mechanical energy, has been utilized as a clinical therapy for bone fracture healing. However, the mechanism how LIPUS affects osteoclast formation and osteoclast activity, has not been fully detailed. Here we found that LIPUS inhibited RANKL-induced osteoclast differentiation in vitro, characterized by decreased number and area of tartrate-resistant acid phosphatase (TRAP) positive cells. Moreover, the expression levels of osteoclast-specific gen...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Co-suppression of VEGF-A and VEGF-C inhibits development of experimental hemangioma.
Authors: Huang C, Huang J, Yu G Abstract Vascular endothelial growth factor A (VEGF-A) plays a critical role in the development and progression of Infantile hemangioma (IH), the most common vascular tumor occurring during infancy. However, a role of VEGF-C in IH remains unclear. Here, we addressed this question. The expression of VEGF family members in hemangiomas at involuting-phase and at proliferating-phase was compared, by RT-qPCR and by ELISA. VEGF-A and VEGF-C were suppressed by specific short-hairpin interfering RNA (shRNA), respectively. Cell growth was determined in an MTT assay. Cell proliferation was ass...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Autologous fibroblasts, peripheral blood mononuclear cells, and fibrin glue accelerate healing of refractory cutaneous ulcers in diabetic mice.
CONCLUSION: Fibrin-sealed cells accelerate wound healing and microvascularization by supplying growth factors, and thus are promising as treatment against refractory cutaneous ulcers. PMID: 30323878 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Live Mycobacterium leprae inhibits autophagy and apoptosis of infected macrophages and prevents engulfment of host cell by phagocytes.
In this study, we treated macrophages with primary M. leprae strains isolated from both lepromatous leprosy (L-lep) and tuberculoid leprosy (T-lep) patients. We found that infection by live M. leprae, regardless of the primary strain, resulted in M2 skewing in the infected macrophage. This skewing was associated with downregulated IRGM expression, a core organizer protein in the autophagy assembly and reduced autophagosome formation, and with lower annexin V staining and lower caspase 3 and caspase 9 activity. Moreover, live M. leprae-infected macrophages prevented efficient phagocytosis by uninfected bystander macrophages...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Peptide nucleic acids targeting mitochondria enhances sensitivity of lung cancer cells to chemotherapy.
Authors: Chen SS, Tu XY, Xie LX, Xiong LP, Song J, Ye XQ Abstract Acquired resistance to chemotherapy is a major limitation for the successful treatment of lung cancer. Previously, we and others showed that formation of tumor spheres is associated with chemotherapy resistance in lung cancer cells, but the underlying mechanisms remained largely unknown. In the current study, we show that mitochondrial activity is significantly higher in A549 tumor spheres versus monolayer cells, establishing mitochondria as a putative target for antitumor therapy. To this end, we designed a peptide nucleic acids (PNAs) coupled with ...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Gd-EOB-DTPA DCE-MRI biomarkers in a rabbit model of liver fibrosis.
Authors: Ji Y, Zhang C, Huang Z, Wang X, Yue L, Gao M, Hu H, Su Q, Han Y, Liu B, Yang D, Su Z, Zhang Z Abstract The purpose of this study was to investigate the correlations between Gd-EOB-DTPA DCE-MRI biomarkers and histopathologic biomarkers of liver fibrosis progression in a rabbit model of liver fibrosis. Thirty-Six New Zealand white rabbits were randomly divided into control (n = 6) and liver fibrosis group (n = 30). Each rabbit in the liver fibrosis group received a weekly subcutaneous injection in the back comprising 50% carbon tetrachloride (CCl4) in oily solution. Control rabbits received subcutaneous inje...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A high circulating FGF21 level as a prognostic marker in patients with acute myocardial infarction.
Authors: Chen H, Lu N, Zheng M Abstract OBJECTIVE: The purpose of this study was to examine the level of serum FGF21 in acute myocardial infarction (AMI) and to explore the association between serum FGF21 and major adverse cardiovascular events in AMI patients. METHODS: A total of 238 Chinese patients were recruited for this study between January 2016 and June 2016, including 183 AMI patients and 55 patients without AMI were enrolled in this study. The level of serum FGF21 was measured by ELISA. Patients were followed-up after admission using a standardized protocol that included Outpatient follow-up and teleph...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Ouabain ameliorates bleomycin induced pulmonary fibrosis by inhibiting proliferation and promoting apoptosis of lung fibroblasts.
In conclusion, our results indicate ouabain a novel effective drug that inhibits lung fibrosis progression. PMID: 30323883 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Intra-carotid arterial transfusion of autologous circulatory derived CD34+ cells for old ischemic stroke patients - a phase I clinical trial to evaluate safety and tolerability.
Authors: Sung PH, Lin HS, Lin WC, Chang CC, Pei SN, Ma MC, Chen KH, Chiang JY, Chang HW, Lee FY, Lee MS, Yip HK Abstract This phase I clinical trial tested the hypothesis that circulatory CD34+ cell therapy might be safe for old ischemic stroke (IS) (defined as IS>6 months) patients and also to evaluate the neurological function after the therapy. Nine old IS patients (with mean IS interval: 8.6 ± 6.4 years) were consecutively enrolled and received intra-carotid artery transfusion of circulatory-derived autologous CD34+ cells (3.0×107 cells/patient) into the ipsilateral brain infarct area at cathete...
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Photochemical crosslinking of caries-affected dentin combined with total- or self-etch systems.
CONCLUSION: Our results, for the first time, are suggesting that etching with phosphoric acid potentialized the benefits of R+UVA crosslinking on carious dentin. R+UVA dentinal collagen crosslinking improves bond strength for caries-affected dentin when using a total-etch adhesive, but did not affect it when using a self-etch adhesive. PMID: 30323885 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

P2X7 receptor regulates sympathoexcitatory response in myocardial infarction rats via NF- κB and MAPK pathways [Retraction].
P2X7 receptor regulates sympathoexcitatory response in myocardial infarction rats via NF-κB and MAPK pathways [Retraction]. Am J Transl Res. 2018;10(9):2996 Authors: Abstract [This retracts the article on p. 4954 in vol. 9, PMID: 29218093.]. PMID: 30323886 [PubMed - in process] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 17, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The critical role of histone lysine demethylase KDM2B in cancer.
Authors: Yan M, Yang X, Wang H, Shao Q Abstract The discovery of histone demethylases has revealed the dynamic nature of the regulation of histone methylation. KDM2B is an important histone lysine demethylase that removes methyl from H3K36me2 and H3K4me3. It participates in many aspects of normal cellular processes such as cell senescence, cell differentiation and stem cell self-renewal. Recent studies also showed that KDM2B was overexpressed in various types of cancers. This review focuses primarily on the current knowledge of KDM2B and its function in cancer development. PMID: 30210666 [PubMed] (Source: Ameri...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

New advances in immunotherapy for non-small cell lung cancer.
Authors: Qin H, Wang F, Liu H, Zeng Z, Wang S, Pan X, Gao H Abstract Immunotherapy is one of the methods that can change the survival rate of patients with malignant tumors, in addition to surgery therapy, radiotherapy, chemotherapy and targeted therapy. Among various immunotherapy methods, immunoprecipitation inhibitors have been the most effective medications developed in recent years. At present, more in-depth studies have been conducted for two immune checkpoint inhibitor pathways, programmed cell death protein 1 (PD-1)/Programmed death-ligand 1 (PD-L1) and cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4), ...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A comprehensive investigation using meta-analysis and bioinformatics on miR-34a-5p expression and its potential role in head and neck squamous cell carcinoma.
Authors: Li J, Liu K, Zhang T, Yang Z, Wang R, Chen G, Kang M Abstract HNSCC is the sixth most common cancer worldwide and is characterized as an aggressive, malignant tumor. MiR-34a-5p (miR-34a) expression has been strongly linked to HNSCC development. However, the exact target gene of miRNA-34a-as well as its biological and mechanistic pathways-are unclear. It is critical that the clinical value of HNSCC receive further study. We conducted a continuous variable, meta-analysis from data found in the cancer literature as well as that provided by the Cancer Genome Atlas (TCGA) to estimate miR-34a expression in HNSCC...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Identification of down-regulated microRNAs in thyroid cancer and their potential functions.
CONCLUSION: Low miRNA expression in thyroid cancer might influence tumorigenesis via critical pathways. The genes identified here may act as a starting point for further investigation of the carcinogenic mechanisms of these miRNAs. PMID: 30210669 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Diagnostic utility of fluorogenic peptide-conjugated Au nanoparticle probe corroborated by rabbit model of mild cartilage injury and panel of osteoarthritic patients.
Authors: Liu Z, Hu X, Yang P, Zhang J, Zhou C, Ao Y Abstract Using a rabbit model of early-stage osteoarthritis (OA) and a sampling patients with OA, we evaluated the diagnostic utility of a fluorogenic peptide-conjugated gold nanoparticle (AuNP) probe in detecting mild cartilage injury, based on the a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) enzyme. Synthesis of this fluorescent turn-on probe (or AU-probe) required conjugation of AuNPs with a fluorescein isothiocyanate (FITC)-modified ADAMTS-4-specific peptide (DVQEFRGVTAVIR). Synovial fluid samples were then collected from 48 adul...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Entresto therapy effectively protects heart and lung against transverse aortic constriction induced cardiopulmonary syndrome injury in rat.
This study tested the hypothesis that entresto therapy effectively protected heart and lung against cardiopulmonary syndrome (CPS) caused by transverse aortic constriction (TAC) in rat. Adult-Male SD rats (n = 36) were equally categorized into group 1 [sham-operated control (SC)], group 2 [SC + enalapril (7 mg/kg/day) since day-28 after TAC induction], group 3 [SC + entresto (30 mg/kg/day) since day-14 after TAC induction], group 4 (TAC only), group 5 (TAC + enalapril) and group 6 (TAC + entresto) and euthanized at day 60 after TAC induction. By day 60, the left-ventricular (LV) ejection fraction was significantly lower in...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Development of genistein-PEGylated silica hybrid nanomaterials with enhanced antioxidant and antiproliferative properties on HT29 human colon cancer cells.
Authors: Pool H, Campos-Vega R, Herrera-Hernández MG, García-Solis P, García-Gasca T, Sánchez IC, Luna-Bárcenas G, Vergara-Castañeda H Abstract The anticancer use of genistein (Gen) has been severely limited due to its low water solubility, low bioavailability, and instability under experimental conditions. To overcome these limitations, we propose a formulation of a hybrid nanomaterial (HNM) based upon the incorporation of Gen into PEGylated silica nanoparticles (PEG-SiNPs) (Gen-PEG-SiHNM), where their physicochemical and biological effects on HT29 cells were evaluated. Ge...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The accurate surgical margin before surgery for malignant musculoskeletal tumors: a retrospective study.
Authors: Hao Y, Yang C, He J Abstract There is no accurate volume measurement method for evaluating the response to chemotherapy in malignant musculoskelal tumors, and there is no specific preoperative evaluation method to evaluate surgical margins. Twenty-five cases of malignant musculoskeletal tumors treated from Mar 2012 to Mar 2014 were analyzed. Through the use of a connective slice-scan and augmented virtual reality technique, accurate measurement of the tumor volume and determination of surgical margins according to the standard proposed by Kawaguchi were easily reached. Specimens were sent for a pathologica...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Direct factor Xa inhibition attenuates acute lung injury progression via modulation of the PAR-2/NF- κB signaling pathway.
Direct factor Xa inhibition attenuates acute lung injury progression via modulation of the PAR-2/NF-κB signaling pathway. Am J Transl Res. 2018;10(8):2335-2349 Authors: Shi M, Wang L, Zhou J, Ji S, Wang N, Tong L, Bi J, Song Y, Hu J, Chen X Abstract The role of coagulation in acute lung injury (ALI) remains unclear. As factor Xa-dependent protease-activated receptor 2 (PAR-2) is reported to be an important target in blood coagulation and other processes, an inhibitor of factor Xa, rivaroxaban, was tested in vivo in C57BL/6 mice with ALI induced by intratracheal injections of lipopolysaccharide (L...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Serum NOX2 as a new biomarker candidate for HBV-related disorders.
In this study, we found that NOX2 values were significantly higher in patients compared to healthy control (P
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The neural regeneration effect of chitin biological absorbable tubes bridging sciatic nerve defects with sural nerve grafts.
CONCLUSIONS: The findings suggest that using a sural nerve graft to repair sciatic nerve defects with chitin biological absorbable tubes bridge promotes structural and functional recovery, and improves end plate and muscle fiber morphology as well. PMID: 30210676 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Transcriptomic profile analysis of brain microvascular pericytes in spontaneously hypertensive rats by RNA-Seq.
CONCLUSIONS: Several DEGs and signaling pathways were identified in the brain microvascular pericytes of SHR rats compared to the WKY rats. Our findings will lay the foundation to study the role of brain microvascular pericytes in the development of spontaneous hypertension. PMID: 30210677 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Angiotensin II receptor blocker reverses heart failure by attenuating local oxidative stress and preserving resident stem cells in rats with myocardial infarction.
In conclusion, ARBs aided cardiac repair post-MI through short-term preservation of stem cells and persistent anti-oxidative stress and anti-senescence effects, partially by attenuating activation of cardiac RAS and restoring the local IGF-1/IGF-1R/Akt pathway. PMID: 30210678 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Extracellular galectin-3 facilitates colon cancer cell migration and is related to the epidermal growth factor receptor.
Authors: Wu KL, Kuo CM, Huang EY, Pan HM, Huang CC, Chen YF, Hsiao CC, Yang KD Abstract We previously found that galectin-3 enhanced DLD-1 cell migration through the K-Ras-Raf-Erk1/2 pathway, but the effect of extracellular galectin-3 on cancer cell migration and its interaction with the epidermal growth factor receptor (EGFR) remained unknown. We aimed to determine the effect of extracellular galectin-3 on colon cancer cell migration and its correlation with the EGFR expression. Western blotting was performed to analyze galectin-3 secretion, shRNA was used to stably knock down galectin-3 expression and a migration...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Measurement of lumbar isthmus parameters for novel starting points for cortical bone trajectory screws using computed radiography.
Authors: Rexiti P, Abudurexiti T, Abuduwali N, Wang S, Sheng W Abstract The current study aims to measure distance parameters in lumbar isthmus to develop new references for lumbar pedicle screw insertion. Using computed radiography, the distance between the median pedicle tangent and the isthmus lateral tangent (D1) and the isometric distance between the isthmus lateral tangent and the inferior border of transverse process (D2) were measured on 120 spine X-ray images. A derived distance D3 (equal to D2 minus 1 mm) was calculated. These parameters were used to define the starting points for pedicle screws. Cortical...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Anticancer effects of plant derived Anacardic acid on human breast cancer MDA-MB-231 cells.
In this study, the anticancer effects of AA on TNBC MDA-MB-231 cells were investigated. The results of our study showed that AA inhibited cell proliferation, induced G0/G1-phase cell cycle arrest, suppressed cell invasion and migration, and induced apoptosis in the MDA-MB-231 cells. Regulation of the key Hsp90-dependent tumor-related molecules or endoplasmic reticulum stress (ERS) related molecules, such as GRP78, Hsp70, CDK-4, MMP-9, Bcl-2, and Mcl-1 by AA may be related to these effects. Taken together, our results suggest that AA shows potential as a possible new drug for therapy of TNBC. PMID: 30210681 [PubMed] (So...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Anesthesia and surgery induce delirium-like behavior in susceptible mice: the role of oxidative stress.
Authors: Zhang J, Gao J, Guo G, Li S, Zhan G, Xie Z, Yang C, Luo A Abstract Anesthesia and surgery (A + S) are risk factors for patients to develop postoperative delirium (POD). However, the pathogenesis of POD remains largely to be determined. We employed battery of behavioral tests including open-filed test (OFT), elevated plus maze test (EPMT) and buried food test (BFT) to investigate the role of oxidative stress in the development of POD and to explore the therapeutic target for POD in mice after A + S (simple laparotomy under 1.4% isoflurane anesthesia). We initially found that 6 hours after A + S, mice failed...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Islet transplantation attenuates cardiac fibrosis in diabetic rats through inhibition of TGF- β1/Smad3 pathway.
Islet transplantation attenuates cardiac fibrosis in diabetic rats through inhibition of TGF-β1/Smad3 pathway. Am J Transl Res. 2018;10(8):2445-2456 Authors: Wang HW, Chen YH, Chen YY, Huang W, Zhu XD, Ni FB, Wu GD, Xu ZQ, Huang ZQ, Chen BC, Xiao FY Abstract Although islet transplantation has been identified as a promising endocrine replacement treatment for patient with diabetes mellitus (DM), it still remains unclear whether islet transplantation can inhibit the diabetic-induced myocardial injury and subsequent adverse ventricular remodeling. Here, we sought to explore the molecular mechanism un...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-144-3p inhibits cell proliferation and induces cell apoptosis in prostate cancer by targeting CEP55.
In this study, we aimed to analyze the role of miR-144-3p in PCa. By RT-qPCR, we found that expression of miR-144-3p was markedly down-regulated in PCa tissues and cell lines compared with that in paired adjacent normal tissues and normal cell lines. Moreover, miR-144-3p overexpression in PC-3 and DU145 cells by transfection with miR-144-3p mimics significantly inhibited cell proliferation and in vitro by MTT, colony formation assays and suppressed tumor growth in vivo by nude mice model. Flow cytometry analysis further demonstrated that forced expression of miR-144-3p induced cell cycle G1/S phase arrest and apoptosis. Mo...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Collagen type V a2 (COL5A2) is decreased in steroid-induced necrosis of the femoral head.
In conclusion, the expression COL5A2 was lower in patients with steroid-induced ONFH, hence COL5A2 may be a promising therapeutic target for steroid induced ONFH treatment. PMID: 30210685 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

E7 peptide-functionalized Ti6Al4V alloy for BMSC enrichment in bone tissue engineering.
In this study, we conjugated E7 peptide to Ti6Al4V alloy to fabricate a scaffold (BTS) to improve the biocompatibility of the alloy. E7 peptide efficiently improved the adhesion of BMSCs to Ti6Al4V alloy. In addition, the BTS scaffold was more conducive to osteogenesis than the RGD-functionalized and non-functionalized control scaffolds. The functional BTS scaffold could pave the way for designing functional joint prostheses, which promote osseointegration between the host bone and implant. PMID: 30210686 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Huangqi (astragalus) decoction ameliorates diabetic nephropathy via IRS1-PI3K-GLUT signaling pathway.
Authors: Chen X, Wang H, Jiang M, Zhao J, Fan C, Wang Y, Peng W Abstract Huangqi decoction (HD) is a prescription for the treatment of diabetes in traditional Chinese medicine. The study was aimed to investigate the effect of HD on diabetic nephropathy. Male diabetic db/db mice which develop diabetic nephropathy spontanously and non-diabetic db/m control mice were used in the current study, and received the treatment of HD for 14 consecutive weeks. HD treatment dose-dependently decreased the body weight, urine volume, water intake and food intake, improved glucose tolerance and insulin resistance, and lowered blood...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Polysaccharide extracted from Portulacae Oleracea L. exerts protective effects against dextran sulfate sodium-induced colitis through inhibition of NF- κB.
Polysaccharide extracted from Portulacae Oleracea L. exerts protective effects against dextran sulfate sodium-induced colitis through inhibition of NF-κB. Am J Transl Res. 2018;10(8):2502-2510 Authors: Wang Z, Wang L, Wu X, Pan YL, Xie P, Pei G, Liang YN, Tang ZS, Liu L Abstract Portulacae Oleracea L. (POL) is a traditional Chinese medicine and also an edible vegetable used to treat diarrhea in china for thousands years. Though the therapeutic effect has been proved in clinical trials, the concrete effective component and mechanisms remained elusive. Polysaccharide from POL has been extracted pre...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Transcription factor Tbx18 induces the differentiation of c-kit+ canine mesenchymal stem cells (cMSCs) into SAN-like pacemaker cells in a co-culture model in vitro.
We examined whether the overexpression of Tbx18 can induce the differentiation of c-kit+ cMSCs into a phenotype similar to that of native pacemaker cells and whether these transfected cells can couple to adjacent atrial cells with functional consequences. The c-kit+ cMSCs were first sorted, then transfected with different lentiviral vectors. Tbx18-c-kit+ cMSCs represented the experimental group, while EYFP-c-kit+ cMSCs and canine sinoatrial node (SAN) cells were used as controls. Within days of transfection, the hyperpolarization-activated cyclic nucleotide-gated (HCN) channel HCN4 protein and gap junction protein Connexin...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Kindlin-2-mediated upregulation of ZEB2 facilitates migration and invasion of oral squamous cell carcinoma in a miR-200b-dependent manner.
Authors: Ren W, Gao L, Qiang C, Li S, Zheng J, Wang Q, Zhi Y, Cai G, Kong X, Zhou M, Qu Z, Zhi K Abstract The miR-200 family suppresses epithelial-mesenchymal transition by inhibiting ZEB1 and ZEB2 mRNA translation in several types of cancers. Kindlin-2 is a target gene of miR-200b and its expression level correlates positively to ZEB2 in oral squamous cell carcinoma (OSCC). Whether Kindlin-2 and ZEB2 share a competitive endogenous RNAs regulatory network in OSCC remains unclear. Here, we studied the expression levels of miR-200b, Kindlin-2, and ZEB2 and found direct interaction between miR-200b, ZEB2, and Kindlin-...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

LOX inhibitor HOEC interfered arachidonic acid metabolic flux in collagen-induced arthritis rats.
Authors: Yang W, Wang X, Xu L, Li H, Wang R Abstract Arachidonic acid (AA) metabolic network generates a variety of products that mediate or modulate inflammatory reactions. (+)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeate (HOEC), isolated from Incarvillea mairei var. granditlora (Wehrhahn) Grierson, was found as an inhibitor of 5-LOX and 15-LOX in vitro. When evaluated in collagen-induced arthritis (CIA) rats, however, lowdose of HOEC (1 mg/kg) showed better efficacy than that of high dose (10 mg/kg). To study how HOEC interfered the AA metabolic pathway, in this study, we dynamically observed the changes of plas...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Apoptosis of bone marrow mesenchymal stromal/stem cells via the MAPK and endoplasmic reticulum stress signaling pathways.
Authors: Chen T, Zhu H, Wang Y, Zhao P, Chen J, Sun J, Zhang X, Zhu G Abstract Therapy for myocardial regeneration using bone marrow stromal cells (BM-MSCs) has been applied to improve the cardiac function of subjects with acute myocardial infarction. However, the study of this therapy has encountered a bottleneck because BM-MSCs are prone to apoptosis in ischemic and anoxic environments. The goal of this study was to investigate the expression of mitogen activated protein kinase (MAPK) (p-38, JNK and ERK) and endoplasmic reticulum stress protein (caspase-12 and CHOP) during BM-MSC apoptosis. In a BM-MSC model of h...
Source: American Journal of Translational Research - September 14, 2018 Category: Research Tags: Am J Transl Res Source Type: research