Cell-free DNA in blood and urine as a diagnostic tool for bladder cancer: a meta-analysis.
CONCLUSIONS: Cell-free DNA has a high diagnostic value in bladder cancer. PMID: 30093933 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Gankyrin as a potential target for tumor therapy: evidence and perspectives.
Authors: Li H, Zhang J, Zhen C, Yang B, Feng L Abstract Gankyrin (also known as PSMD10 or p28GANK), engages in diverse biological processes, including cellular growth, proliferation and invasion. Several studies have demonstrated that Gankyrin is a candidate oncogene. In parallel, the dysregulation of Gankyrin has been observered in a variety of human cancer. Overexpression of Gankyrin is involved in tumor initiation and progression by regulating several signaling pathways that control cell-cycle process, cell growth, apoptosis, et al. On the contrary, downregulation of Gankyrin significantly inhibits cell growth, ...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Resveratrol alleviates sepsis-induced acute lung injury by suppressing inflammation and apoptosis of alveolar macrophage cells.
Authors: Yang L, Zhang Z, Zhuo Y, Cui L, Li C, Li D, Zhang S, Cui N, Wang X, Gao H Abstract Sepsis is a major cause of death in intensive care units. The purpose of this study was to investigate the effect of resveratrol (RSV) on sepsis-induced acute lung injury (ALI). The underlying molecular mechanisms were deciphered by both in vitro and in vivo experiments. Polymicrobial sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). RSV pretreatment significantly attenuated CLP-induced acute lung injury, which was associated with enhanced expression of VEGF-B. The protective properties of RSV were ass...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Valproic acid regulates Ang II-induced pericyte-myofibroblast trans-differentiation via MAPK/ERK pathway.
This study focused on the role of HDACs in pericyte-myofibroblast trans-differentiation and how HDACs inhibitor VPA influenced proliferation, migration, viability and myofibroblast trans-differentiation of pericytes for the first time. Rat cardiac fibrosis model was induced by Ang II. Immunohistochemistry was employed to examine cardiac fibrosis and flow cytometry was used to analyze whether inflammatory cells involve VPA-induced trans-differentiation. Pericytes proliferation, migration and differentiation to myofibroblasts were performed to examine the role of VPA on pericyte trans-differentiation. Immunoblot and qPCR wer...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells.
Authors: Nicolau Y, Bany-Mohammed F, Cai CL, Aranda JV, Beharry KD Abstract Vascular endothelial growth factor (VEGF) is a potent mitogen that regulates proliferation, migration, and tube formation of endothelial cells (EC). VEGF has recently become a target for severe retinopathy of prematurity (ROP) therapy. We tested the hypothesis that a specific VEGF isoform and/or receptor acts synergistically with insulin-like growth factor (IGF)-I to alter normal retinal microvascular EC angiogenesis and RNA interference can be used to reverse VEGF effects. We used small interfering RNA (SiRNA) transfection to target VEGF i...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Prognosis of patients with TX stage differentiated thyroid cancer: propensity scored matching analysis of the SEER database 2004-2013.
Authors: Xiong Y, Zhao Q, Liu C, Wang S, Liu Z, Huang T Abstract In differentiated thyroid cancer (DTC), TX stage is defined as 'primary tumour cannot be assessed'. The prognosis of patients with TX stage remains unclear. The aim of this study was to investigate the prognosis of TX stage and provide a perspective on treatment guidelines. We investigated a large cohort of DTC patients from the Surveillance, Epidemiology, and End Results database between 2004 and 2013. Patient mortality was examined by Kaplan-Meier analyses with log-rank tests and Cox proportional hazards regression analyses. The rate of cancer-speci...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Diabetes aggravates acute pancreatitis possibly via activation of NLRP3 inflammasome in db/db mice.
Authors: Gao L, Lu GT, Lu YY, Xiao WM, Mao WJ, Tong ZH, Yang N, Li BQ, Yang Q, Ding YB, Li WQ Abstract Clinical studies have confirmed that patients with diabetes had an elevated risk of acute pancreatitis (AP) and diabetes was associated with increased severity and mortality in patients with AP. However, these studies failed to prove a cause-and-effect relationship between diabetes and AP. In the present study, we for the first time have evaluated the effects of diabetes on AP by adopting a type 2 diabetes animal model db/db mice and investigated the possible underlying mechanisms. The results showed that in compa...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Exosomes derived from human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease in mice through ubiquitination.
In this study, we aimed to investigate the therapeutic effect and probe the mechanism relating to ubiquitination underlying the hucMSC-ex treatment in DSS-induced IBD of mice. HucMSC-ex significantly improved the symptoms of IBD in mice. In the IBD group, the gene expression levels of TNF-α, IL-1β, IL-6, NAe1, E2M and Uba3 dramatically increased while those of IL-10 and IP-10 decreased. The gene expression level in the group of hucMSC-ex treatment was adversed to that in the group of IBD. In the hucMSC-ex treated group, western blot results showed that the protein expressions of K48, K63 and FK2 have significant...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Edaravone ameliorates experimental autoimmune thyroiditis in rats through HO-1-dependent STAT3/PI3K/Akt pathway.
Authors: Li H, Min J, Mao X, Wang X, Yang Y, Chen Y Abstract Autoimmune thyroiditis is among the most prevalent of all the autoimmunities in population. It is characterized as both cellular immune responses with T, B cells infiltrating to the thyroid gland followed by hypothyroidism as a result of destruction of the thyroid follicles and fibrous replacement of the parenchymal tissue, as well as immune response for TPO and Tg-antibody production. Experimental autoimmune thyroiditis (EAT) has been proven to be an ideal model to study autoimmune thyroiditis. In the present study, we induced an EAT model in rats and ex...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

pre-B cell colony enhancing factor negatively regulates Na+ and fluid transport in lung epithelial cells.
This study was undertaken to investigate the effect of pre-B cell colony enhancing factor (PBEF) on Na+ and fluid transport in lung epithelial cells. METHODS: Type 1 and 2 cells were isolated from lung epithelium. After hypoxia reoxygenation treatment, the primary cell cultures were transfected with a plasmid over-expressing PBEF. Sodium-potassium ATPase (NKA), epithelial sodium channel (ENaC), type I cell marker rT140, surfactant protein (SP) and PBEF protein were analyzed at mRNA and protein levels using PCR and Western blot analysis. Immunofluorescence assays showed type 1 and 2 cells were successfully isolated. Aft...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The molecular mechanism and clinical significance of LDHA in HER2-mediated progression of gastric cancer.
CONCLUSIONS: The HER2-HIF-1α-LDHA axis may serve as the basis for new methods and strategies for the treatment of GC. PMID: 30093943 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Comparative study of nanostructured carriers of calcium phosphate and magnesium phosphate loaded with SRT1720 for the protection of H2O2-induced senescent endothelium.
Authors: Su ZX, Shi YQ, Lu ZY, Li RL, Wang XL, Ning BB, Duan JL, Hao LS, Duan JH, Li Y, Zhu YJ, Hao CN, Wang R Abstract Nanostructured calcium phosphate (CaP) and magnesium phosphate (MgP) are promising for the application as the nanocarriers in drug delivery. However, the difference between CaP and MgP nanocarriers in drug delivery is rarely investigated. In this work, we comparatively investigated nanostructured CaP, MgP and calcium magnesium phosphate (CMP) for the delivery of SRT1720, which is a silent information regulator (SIRT1) specific activator with pro-angiogenic and anti-aging properties in response to ...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Celastrol aggravates LPS-induced inflammation and injuries of liver and kidney in mice.
In this study, we investigated the effect of celastrol on lipopolysaccharides (LPS)-induced inflammation and organ injuries in mice. Following celastrol pretreatment, mice showed increased mortality rate and aggravated inflammation evidenced by further enhanced inflammatory markers of IL-6, IL-1β, TNF-α, IL-18, MCP-1, and ICAM-1 in circulation, liver, and kidney after LPS treatment. The serum levels of ALT, AST, and LDH were further increased in parallel with the deteriorated liver morphological damage (H&E) and oxidative stress in celastrol-treated mice, indicating an aggravated liver injury. In kidney, the...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The change of synovial fluid proteome in rabbit surgery-induced model of knee osteoarthritis.
Authors: Luo Q, Qin X, Qiu Y, Hou L, Yang N Abstract The aims of this study were to explore the change of synovial fluid (SF) proteome in a knee osteoarthritis (KOA) rabbit model, and to provide a new target for the treatment of knee osteoarthritis at the proteomic level. Sixteen New Zealand rabbits were randomly and equally divided into two groups. Group A rabbits were subjected to right anterior cruciate ligament transection (ACLT), while group B rabbits were subjected to sham ACLT. Six weeks later, the proteomes of knee joint SF from group A and B rabbits were analyzed using a label-free quantitative proteomic a...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis.
In conclusion, hAMSC and CM administration ameliorated biliary hyperplasia, peribiliary fibrosis, and inflammation in a rat model of sclerosing cholangitis. hAMSCs and CM may represent new modalities for treating sclerosing cholangitis. PMID: 30093947 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Urinary proteomics analysis based on mass spectrometry and identification of therapeutic targets of Shenkangling interventions in rats with adriamycin nephropathy using iTRAQ.
CONCLUSION: The feasibility of urinary proteomics analysis in rats using a large number of proteins with finite molecular weights is controversial. The markers screened in this study may be of clinical value for the diagnosis and treatment of nephropathy. However, these findings should be confirmed in future cohort studies. PMID: 30093948 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Bioinformatics-based analysis of the involvement of AC005550.3, RP11-415D17.3, and RP1-140K8.5 in homocysteine-induced vascular endothelial injury.
In conclusion, downregulated AC005550.3 and RP11-415D17.3 targeting CD34 and FGF2 and upregulated RP1-140K8.5 targeting SERPINE1 may play an important role in HCY-induced vascular endothelial injury by regulating blood vessel development and fibrinolysis, respectively. PMID: 30093949 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Uremia induces upregulation of cerebral tissue oxidative/inflammatory cascade, down-regulation of Nrf2 pathway and disruption of blood brain barrier.
In conclusion CKD results in the cerebral tissue activation of inflammatory and oxidative pathways, inhibition of antioxidant and cytoprotective system and erosion of cerebral capillary junctional complex, events that contribute to CNS dysfunction and impaired blood brain barrier. PMID: 30093950 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Emerging role of C5a/C5aR IL-17A axis in cGVHD.
Authors: Chen X, Lai P, Wang Y, He C, Wu S, Huang X, Geng S, Luo C, Ling W, Zeng L, Li P, Jiang Z, Weng J, Du X Abstract Chronic graft-versus-host disease (cGVHD) manifests with features characteristic of autoimmune disease with organs attacked by pathogenic Th17 cells. However, the mechanism of Th17 cells generation in the setting of cGVHD is still unclear. Here we defined C5a/C5aR-IL-17Aaxis as a novel signaling that required in the pathologies of cGVHD. We firstly found a positive link between complement activation and the Th17 cells in patients with cGVHD. C5a, a critical component of complements, promoted the ...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Use of expanded deltopectoral skin flaps for facial reconstruction after sizeable benign tumor resections.
Authors: Ding J, Li Y, Li W, Liu C, Liu H, Cui J, Su Y, Ma X Abstract The overall unsightliness of expansive benign facial tumors imposes both physical and mental suffering. Although excision is generally the optimal recourse in such instances, reconstructing the subsequent surgical defects is always a critical issue. Herein, we have described our experiences using expanded deltopectoral skin flaps to manage large facial wounds after excising benign tumors. Our endeavor called for retrospective review of 22 patients presenting between July 2007 and March 2017 with various facial growths, including hemangiomas, nevi...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Assessing the impact of cigarette smoking on β-cell function and risk for type 2 diabetes in a non-diabetic Chinese cohort.
Assessing the impact of cigarette smoking on β-cell function and risk for type 2 diabetes in a non-diabetic Chinese cohort. Am J Transl Res. 2018;10(7):2164-2174 Authors: Chen C, Tu YQ, Yang P, Yu QL, Zhang S, Xiong F, Wang CY Abstract Although the impact of cigarette smoking on glucose homeostasis has been extensively studied, the results, however, are still not conclusive. We, therefore, conducted a cross-sectional analysis of a non-diabetic Chinese cohort collected by the China Health and Nutrition Survey (CHNS 2009) to comprehensively assess the relationship between smoking, Hemoglobin A1c, &b...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Clinical efficacy of ticagrelor in patients undergoing emergency intervention for acute myocardial infarction and its impact on platelet aggregation rate.
This study aims to investigate the clinical efficacy of ticagrelor in patients who underwent emergency percutaneous coronary intervention (PCI) for acute myocardial infarction (AMI), and its impact on platelet aggregation rate. METHODS: A total of 257 AMI patients who underwent emergency PCI in our hospital were included in the present study. These patients were randomly divided into two groups: ticagrelor group (n = 129), patients took 180 mg of ticagrelor (qd) before the intervention, and subsequently took 90 mg of ticagrelor (bid) for maintenance; clopidogrel group (n = 128), patients took 300 mg of clopidogrel (qd)...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Evaluation of the safety and tolerability of tamoxifen for ischemia-incited renal injury in mice.
Authors: Wang Y, Zhao Z, Yang W, Li L, Zhu F, Pei G, Yang J, Zhu H, Xu H, Wang M, Yang Q, Hu Z, Wang P, Xu G, Zeng R, Yao Y Abstract Tamoxifen is used to activate tamoxifen-dependent Cre recombinase (CreER) to generate time- and tissue-specific genetically mutant mice. However, tamoxifen is also an active estrogen analogue that binds with higher affinity to estrogen receptors and exhibits anti-apoptosis, anti-inflammation, and antifibrotic properties. Renal ischemia reperfusion (I/R) injury is characterized by increased apoptosis and inflammation, so optimal utility of tamoxifen-inducible CreER genetic systems in I...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Vincamine prevents lipopolysaccharide induced inflammation and oxidative stress via thioredoxin reductase activation in human corneal epithelial cells.
In this study, the potential protective effects of vincamine, a plant alkaloid used clinically as a peripheral vasodilator, against LPS induced inflammation and oxidative stress were investigated on human corneal epithelial cells (HCECs). HCECs were treated with LPS and vincamine at various concentrations. Cell viability, reactive oxygen species (ROS) levels, and the gene expression levels of interleukin-6 (IL-6), IL-8, IL-1β, TNF-α, transforming growth factor-β (TGF-β) in HCECs, were assessed. The antioxidant potential of vincamine was evaluated by measuring the levels of malondialdehyde (MDA), total ...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A novel probe for measuring tissue bioelectrical impedance to enhance pedicle screw placement in spinal surgery.
Authors: Li Z, Chen C, Lin Y, Li X, Tan H, Chan MT, Wu WK, Zhan S, Cao Q, Shen J Abstract Posterior spinal reconstruction with rods and pedicle screws has been widely used to corrects coliosis and other forms of degenerative spinal deformities. However, insertion of pedicle screwsis often clinically challenging, particularlyin patients with severe deformity. Bioelectrical impedance analysis is a technique that exploits the electrical properties of biological organs and tissues to indicate their compositions. Bioelectrical impedance measurement is non-invasive, simple, with adequate repeatability, and at a relativel...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Research progress of the relationship between pyroptosis and disease.
Authors: Ma Y, Jiang J, Gao Y, Shi T, Zhu X, Zhang K, Lu K, Xue B Abstract Pyroptosis, characterized by proinflammation, has been defined as a new type of programmed cell death in recent years. Inflammasomes are activated by the corresponding pathogen-associated molecular patterns (PAMPS) or damage-associated molecular patterns (DAMPS), followed up by the cleavage of pro-interleukin-1β (pro-IL-1β), pro-interleukin-18 (pro-IL-18) and gasdermin D. The N-terminal fragment of gasdermin D gives rise to the destruction of cell membrane, leading to cell rupture as well as the efflux of proinflammatory cytokines....
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Erratum: Protease activated receptor 2 in diabetic nephropathy: a double edged sword.
Authors: Waasdorp M, Duitman JW, Florquin S, Spek CA Abstract [This corrects the article on p. 4512 in vol. 9, PMID: 29118913.]. PMID: 30093959 [PubMed - in process] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Erratum: Exogenous glutathione contributes to cisplatin resistance in lung cancer A549 cells.
Authors: Lan D, Wang L, He R, Ma J, Bin Y, Chi X, Chen G, Cai Z Abstract [This corrects the article on p. 1295 in vol. 10, PMID: 29887946.]. PMID: 30093960 [PubMed - in process] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The progress of circular RNAs in various tumors.
Authors: Wang D, Yang S, Wang H, Wang J, Zhang Q, Zhou S, He Y, Zhang H, Deng F, Xu H, Zhong S, Fu L, Tang J Abstract Circular RNAs (circRNAs), a novel type of non-coding RNAs, presented as covalently closed continuous loops. Recent researches had found that circRNAs could function as microRNA sponges, regulators of gene transcription and encoding proteins. They were relatively stable and expressed widely in cytoplasm, which played important roles in carcinogenesis of cancers, such as esophageal cancer, gastric cancer, colorectal cancer, hepatocarcinoma, bladder cancer, glioma, breast cancer, osteosarcoma and so on...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The lentiviral-mediated Nurr1 genetic engineering mesenchymal stem cells protect dopaminergic neurons in a rat model of Parkinson's disease.
This study examines the therapeutic effects of transplantation of Nurr1 gene-modified bone marrow mesenchymal stem cells (MSCs) on 6-hydroxydopamine (6-OHDA)-induced PD rat models. MSCs were transduced with lentivirus expressing Nurr1 gene and then intrastriatally transplanted into PD rats. Our results showed that Nurr1 gene-modified MSCs overexpress and secrete Nurr1 protein in vitro and also survive and migrate in the brain. Four weeks after transplantation Nurr1 gene-modified MSCs dramatically ameliorated the abnormal behavior of PD rats and increased the numbers of tyrosine hydroxylase (TH)-positive cells in the substa...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

An immune-competent rat split thickness skin graft model: useful tools to develop new therapies to improve skin graft survival.
Authors: Zhai Q, Zhou F, Ibrahim MM, Zhao J, Liu X, Wu J, Chen L, Qi S Abstract Skin grafting is the routine standard of care to manage third degree burns and problematic skin defects. Several commercially available dermal substitutes and biologic skin equivalents are placed in the wound bed to facilitate the healing process of the skin grafts, as well as to provide mechanical support for the cells to grow and to delay the contracture. To study pathology and develop new therapies, an immune-competent rat model is required. We have created two different skin graft animal models to mimic the clinical skin grafting op...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Endovascular treatment for hemorrhagic cerebral venous sinus thrombosis: experience with 9 cases for 3 years.
CONCLUSION: EVT is an effective and safe procedure for potentially catastrophic hemorrhagic cerebral venous sinus thrombosis. PMID: 30018704 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Egr1 deficiency disrupts dynamic equilibrium of chondrocyte extracellular matrix through PPAR γ/RUNX2 signaling pathways.
CONCLUSION: Egr1 has an important regulatory effect on the dynamic equilibrium of the chondrocyte extracellular matrix, which may be achieved through the PPARγ/RUNX2 signaling pathways. PMID: 30018705 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Identification of proteins interacting with pORF5 in the pathogenesis of C. trachomatis.
CONCLUSION: Identification of key proteins interacting with pORF5 could contribute to the understanding and further exploration of the function of pORF5 in the pathogenic mechanisms of C. trachomatis. PMID: 30018706 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Exosome-related lncRNAs as predictors of HCC patient survival: a prognostic model.
Authors: Hou Y, Yu Z, Tam NL, Huang S, Sun C, Wang R, Zhang X, Wang Z, Ma Y, He X, Wu L Abstract OBJECTIVES: Accumulating evidence suggests that long non-coding RNA (lncRNA) may affect hepatocellular carcinoma (HCC) progression. However, the mechanism remains unclear. Previous studies have shown that exosomes may promote tumor progression by transporting proteins. Our study aimed to determine the prognostic value of lncRNAs in HCC and the underlying mechanism. METHODS: A dataset comprising a HCC cohort of 364 patients from The Cancer Genome Atlas (TCGA) was analyzed to identify lncRNAs with prognostic value. Co...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Topiramate exhibits anti-tumorigenic and metastatic effects in ovarian cancer cells.
Authors: Xu G, Fang Z, Clark LH, Sun W, Yin Y, Zhang R, Sullivan SA, Tran AQ, Kong W, Wang J, Zhou C, Bae-Jump VL Abstract Ovarian cancer is one of the leading causes of cancer related deaths among women worldwide, with an overall 5-year survival of only 30-40%. Carbonic anhydrases are up-regulated in many types of cancer and play an important role in tumor progression and metastasis. Carbonic anhydrase 9 has been implicated as a potential anti-tumorigenic target. Topiramate (TPM) is a potent inhibitor of carbonic anhydrase isozymes, including carbonic anhydrase 9, and has been shown to have anti-tumorigenic activi...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Interleukin enhancer binding factor 2 is a prognostic biomarker for breast cancer that also predicts neoadjuvant chemotherapy responses.
Authors: Jin Z, Xu L, Zhang L, Zhao M, Li D, Ye L, Ma Y, Ren S, Yu H, Wang D, Liang C, Chen B Abstract Interleukin enhancer binding factor 2 (ILF2) participates in several aspects of DNA and RNA metabolism and regulates gene expression at multiple levels; however, its role in breast cancer remains undefined. The variant statuses of ILF2 in human breast cancer were evaluated using the COSMIC database. Altered ILF2 expression in normal breast tissue relative to cancer tissue and in breast cancer patients with different clinicopathological characteristics, molecular subtypes, clinical outcomes and chemotherapy respons...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Hsa_circ_0103809 promotes cell proliferation and inhibits apoptosis in hepatocellular carcinoma by targeting miR-490-5p/SOX2 signaling pathway.
CONCLUSION: Our findings suggested that hsa_circ_0103809 might facilitate HCC malignant progression, at least partially, by regulating miR-490-5p/SOX2 signaling pathway. PMID: 30018710 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

HOXB5 promotes retinoblastoma cell migration and invasion via ERK1/2 pathway-mediated MMPs production.
In this study, we found that HOXB5 expression is upregulated in retinoblastoma cell lines and tissues. Overexpression of HOXB5 promoted retinoblastoma cell migration and invasion, but knockdown of HOXB5 suppressed the migration and invasion. Moreover, HOXB5 induced the activation of ERK1/2 and upregulated the production of MMP-3 and MMP-13. In addition, ERK1/2 pathway was required for HOXB5-mediated retinoblastoma cell migration and invasion. Taken together, our study suggests that HOXB5 promotes the migration and invasion of retinoblastoma cells by inducing the activation of ERK1/2 and increasing the production of MMP-3 a...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

NADH protect against radiation enteritis by enhancing autophagy and inhibiting inflammation through PI3K/AKT pathway.
Authors: Wang JT, Xie WQ, Liu FQ, Bi Y, Zhu XJ, Wang QE, Zheng YF Abstract Radiotherapy is an important method for cancer treatment but it has serious side-effects at high doses. One of the greatest challenges in radiotherapy is that radiation affects both healthy tissue and cancer tissues. For abdominal or pelvic lesions, the bowel is the most easily injured by irradiation. Radiation may cause radiation enteritis, intestinal inflammatory infiltration, or intestinal perforation. Coenzyme NADH involves in energy metabolism and transportation of nucleic acid, proteins and carbohydrates. In our study, NADH was used to...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Repair mechanism of mesenchymal stem cells derived from nasal mucosa in orbital fracture.
CONCLUSION: The present study suggested that the repair effect of nasal mucosa-derived MSCs in orbital fracture is achieved by facilitating the expressions of osteogenic markers Runx2, OCN, OPN and BSP. However, the pathways of actions are unknown and further studies are required to elucidate the concrete mechanism. PMID: 30018713 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Application of three-dimensional visualization technique in preoperative planning of progressive hilar cholangiocarcinoma.
CONCLUSION: For patients with progressive hilar cholangiocarcinoma, the optimized three-dimensional visualization technique can accurately demonstrate the dilated biliary tract system, provide a new standard to determine the presence of tumor and peripheral vascular invasion, help in establishing a reasonable individualized operation plan, reduce the incidence of bile leakage and liver failure after the operation, and improve the success rate of operation. PMID: 30018714 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Immunization with glypican-3 nanovaccine containing TLR7 agonist prevents the development of carcinogen-induced precancerous hepatic lesions to cancer in a murine model.
Authors: Chen K, Wu Z, Zang M, Wang C, Wang Y, Wang D, Ma Y, Qu C Abstract BACKGROUND: Glypican-3 (GPC3) is one of the key tissue markers that could discriminate malignant precancerous lesions from benign hepatic lesions in cirrhotic patients. We aimed to develop a GPC3 cancer vaccine to induce specific T cells to intervene in hepatocellular carcinoma (HCC) development. METHODS: Synthesizing mannosylated liposomes (LPMan) as vaccine delivery system, incorporating one Toll-like receptor (TLR)-7/8 agonist CL097 as adjuvant, we prepared a GPC3 nanovaccine, LPMan-GPC3/CL097. We injected 25 mg/kg diethylnitrosamine ...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Catalpol alleviates renal damage by improving lipid metabolism in diabetic db/db mice.
CONCLUSION: Catalpol has a renal protective effect in diabetic db/db mice. PMID: 30018716 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

GAG and collagen II attenuate glucocorticoid-induced osteoporosis by regulating NF- κB and MAPK signaling.
In this study, we aimed to determine the concrete effect and the mechanism of GAG and collagen II on glucocorticoid-induced osteoporosis. We implanted prednisolone pellets subcutaneously in mice to mimic glucocorticoid-induced osteoporosis. GAG was administered intragastrically every day for 60 days. The results demonstrated a protective effect of GAG and collagen II on glucocorticoid-induced osteoporosis. Trabecular number and connection density increased after treatment with GAG and collagen II. We generated bone marrow-derived macrophages to explore the effect of GAG and collagen II on osteoclast differentiation. We col...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Ginkgo biloba extract 761 enhances 5-fluorouracil chemosensitivity in colorectal cancer cells through regulation of high mobility group-box 3 expression.
Authors: Chen X, Zeng L Abstract Although the standard ginkgo biloba extract EGb 761 exhibits antioxidative, anti-apoptotic, and anticancer properties, there is no research focusing on the chemopreventive effects of EGb 761 in colorectal cancer (CRC). The present study investigated whether EGb 761 could increase 5-fluorouracil (5FU) sensitivity in CRC and its potential mechanism. We found that combined EGb 761 and 5FU treatment significantly elevated the chemosensitivity of CRC cells to 5FU in 5FU-resistant (5FUR) CRC cells, whereas no obvious cytotoxicity of EGb 761 was observed in parental cells. Then, real-time ...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

FOXK1 promotes cell growth through activating wnt/ β-catenin pathway and emerges as a novel target of miR-137 in glioma.
In conclusion, the present study demonstrated that FOXK1 promoted cell growth through activating wnt/β-catenin pathway and is negatively regulated by miR-137 in glioma. PMID: 30018719 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

PKM2 functions as a potential oncogene and is a crucial target of miR-148a and miR-326 in thyroid tumorigenesis.
In this study, we investigated the biological function of pyruvate kinase M2 (PKM2) and its regulation by deregulated microRNAs (miRNAs) in thyroid cancer (TC). The mRNA and protein expression of PKM2 was examined by quantitative reverse transcription PCR and western blot. The biological role of PKM2 was demonstrated through small interfering RNA-mediated knockdown experiments. The regulation of PKM2 by miR-148a and miR-326 was confirmed by western blot, dual luciferase activity assays, and rescue experiments. PKM2 was overexpressed in TC tissues and cell lines. The knockdown of PKM2 in TC cells suppressed cell proliferati...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Activation of the CXCL16/CXCR6 pathway promotes lipid deposition in fatty livers of apolipoprotein E knockout mice and HepG2 cells.
This study aimed to investigate the role of CXC chemokine ligand 16 (CXCL16) and its receptor CXC chemokine receptor 6 (CXCR6) in NAFLD under inflammation. We used IL-1β stimulation in human hepatoblastoma cell line (HepG2) for in vitro studies and casein injection in apolipoprotein E knockout mice in vivo to induce inflammatory stress. The effects of inflammation on cholesterol accumulation were examined by histochemical staining and a quantitative intracellular cholesterol assay. The gene and protein expression of molecules involved in CXCL16/CXCR6 pathway and extracellular matrix (ECM) were examined by real-time po...
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Apolipoprotein B-100 peptide 210 antibody inhibits atherosclerosis by regulation of macrophages that phagocytize oxidized lipid.
In conclusion, the anti-atherosclerotic effect of P210-Ab is related to its preferential inhibition of inflammation and reversion of cholesterol transportation by altering the pathway by which macrophages phagocytize ox-LDL. PMID: 30018722 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - July 19, 2018 Category: Research Tags: Am J Transl Res Source Type: research