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Post-translational modifications of high mobility group box 1 and cancer.
Authors: Richard SA, Jiang Y, Xiang LH, Zhou S, Wang J, Su Z, Xu H Abstract Post-translational modifications (PTMs) of High mobility group box 1 (HMGB1) have not been investigated as extensively as those of other HMG proteins but accumulating evidence has shown the remarkable biological significances induced by the post-translational: acetylation, methylation and phosphorylation, oxidation, glycosylation and ADP-ribosylation of the HMGB1 to modulate its interactions with DNA and other proteins. Although HMGB1 is localized in the nucleus in almost all cells at baseline, it can be rapidly mobilized to other sites wit...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Multifunctionalization of graphene and graphene oxide for controlled release and targeted delivery of anticancer drugs.
Authors: Liu CC, Zhao JJ, Zhang R, Li H, Chen B, Zhang LL, Yang H Abstract Among various nanomaterials, graphene and its derivatives have attracted considerable research interest in diverse application areas-including nanomedicine-because of their extraordinary physical, chemical, and optical properties. Intensive research is underway to investigate the biomedical application of graphene and graphene-based nanosystems as drug-delivery vehicles for cancer therapy, and this is considered as one of the novel therapeutic approaches for performing on-demand chemotherapy coupled with photothermal therapy or photodynamic ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

SS31 therapy effectively protects the heart against transverse aortic constriction-induced hypertrophic cardiomyopathy damage.
This study tested the hypothesis that SS31 therapy could effectively protect the heart against transverse aortic constriction (TAC)-induced hypertrophic cardiomyopathy (HCM) damage. Adult-male B6 mice (n=36) were equally divided into sham-operated control (group 1), TAC only (group 2) and TAC+SS31 (group) (2.0 mg/kg/day by intra-peritoneal administration from day 28 after TAC induction) and euthanized by day 60. In vitro results showed that SS31 markedly suppressed angiotensin-II induced protein expressions of BNP/β-MHC, ATM, p-P38 and P53 and ATP damage in H9C2 cells, and protein expression of pro-collagen-I/CTGF in fibroblasts (all P
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The effects of oral supplementation of spirulina platensis microalgae on hematological parameters in streptozotocin-induced diabetic rats.
Authors: Nasirian F, Mesbahzadeh B, Maleki SA, Mogharnasi M, Kor NM Abstract It is shown that diabetes can change hematological parameters and some microalgae, i.e. Spirulina platensis, could improve hematological parameters in non-diabetic rats. The purpose of this study was to investigate the effects of Spirulina platensis (SPM) microalgae on hematological parameters in diabetic rats induced by Streptozotocin. Rats, 2.5 males old, were grouped into two sections including healthy and diabetic and received orally 15 and 30 mg/kg body weight SPM for 5 weeks. Control rats received 0.3 ml of distilled water. The exper...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Dexmedetomidine mitigates CLP-stimulated acute lung injury via restraining the RAGE pathway.
CONCLUSION: Our studies demonstrated that DEX attenuates the aggravation of sepsis-stimulated ALI via down regulation of RAGE pathway, which has a potential value in the clinical therapy. PMID: 29312480 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

AMD3100 inhibits brain-specific metastasis in lung cancer via suppressing the SDF-1/CXCR4 axis and protecting blood-brain barrier.
In this study, strong expression of SDF-1 was observed in highly metastatic brain tissues, and CXCR4 overexpressed in PC-9 lung cancer cells and tumor foci. Therefore, we chose to block SDF-1/CXCR4 axis with AMD3100, which led to the increased tight junction protein level, less damage, and decreased permeability of blood-brain barrier (BBB). Consequently, the process of lung cancer metastasis to the brain was significantly slowed down. These findings were further validated by in vivo experiments, which showed that AMD3100 can effectively inhibit lung cancer brain metastasis and extend the survival of nude mice model, sugge...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Assessment of doxorubicin-induced mouse testicular damage by the novel second-harmonic generation microscopy.
This study employs the SHIM and Western-blot (WB) to assess the cellular-molecular levels of doxorubicin (Dox)-induced mouse testicular damage. The SHIM examination was able to clearly identify the integrity of normal architecture of the living mouse testis, namely, the anatomical features of SM, smooth muscle wall of SM, manchette microtubules, exoplasmic microtubules in Sertoli cells and interstitial connective tissue, as well as the destructive feature of SM in Dox-treated mice (n = 6 per group). By day 21 after Dox-treatment, the testicular weight and testicular length were significantly progressively decreased as Dox ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction.
Authors: Ge L, Cao C, Chen S, Shao B, Li Q, Li Q, Liu L, Liang Z, Huang Y Abstract Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. A common strategy for POP reconstruction is to use scaffold materials to reconstruct the prolapsed pelvic organ. However, the existing materials for pelvic reconstruction do not meet the clinical requirements for biocompatibility, mechanics and immunological rejection. To address these concerns, urinary bladder decellularized materials (UBDM) was selected due to their good strain-stress resistance. To enhance its biocompatibility, laminin/nidogen was...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

FGF21 inhibitor suppresses the proliferation and migration of human umbilical vein endothelial cells through the eNOS/PI3K/AKT pathway.
In this study, we investigated molecular mechanism underlying the regulation of endothelial nitric oxide synthase (eNOS) expression by fibroblast growth factor 21 (FGF21). We analyzed FGF21 and eNOS expression in hypertensive and healthy (control) subjects (n=30/group). To evaluate the effects of FGF21 on endothelial cells, we transfected FGF21 mimics or FGF21 inhibitor into human umbilical vein endothelial cells (HUVECs). Cell proliferation was analyzed using the methyl thiazolyl tetrazolium assay, and cell migration and invasion were assessed using Transwell assays. In addition, eNOS, PI3K, and AKT mRNA in the HUVECs wer...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Combined preconditioning with hypoxia and GYKI-52466 protects rats from cerebral ischemic injury by HIF-1 α/eNOS pathway.
Combined preconditioning with hypoxia and GYKI-52466 protects rats from cerebral ischemic injury by HIF-1α/eNOS pathway. Am J Transl Res. 2017;9(12):5308-5319 Authors: Yang Y, Lu F, Zhuang L, Yang S, Kong Y, Tan W, Gong Z, Zhan S Abstract Cerebral ischemic injury has been the leading cause of death and long term disability in the world because of the lack of successful therapies to it, leading to neurological and behavioral deficits. The present study aims to investigate the effects of combined preconditioning (PC) with hypoxia and GYKI-52466 (GYKI) on cerebral ischemic injury and to explore the ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Interleukin-25 enhances the capacity of mesenchymal stem cells to induce intestinal epithelial cell regeneration.
CONCLUSION: IL-25R is involved in Th-related mucosal inflammation and proliferation of intestinal stem cells in IBD. IL-25 enhances the capacity of MSC to induce intestinal epithelial cell regeneration, and MSC therapy with IL-25 may be a new direction for IBD treatment. PMID: 29312486 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MiR-514a-3p inhibits cell proliferation and epithelial-mesenchymal transition by targeting EGFR in clear cell renal cell carcinoma.
CONCLUSIONS: MiR-514a-3p is a novel tumor suppressor in ccRCC and potentially functions through EGFR/MAPK/ERK pathway. PMID: 29312487 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Dermal V γ4+T cells enhance the IMQ-induced psoriasis-like skin inflammatidon in re-challenged mice.
Dermal Vγ4+T cells enhance the IMQ-induced psoriasis-like skin inflammatidon in re-challenged mice. Am J Transl Res. 2017;9(12):5347-5360 Authors: Zhu R, Cai X, Zhou C, Li Y, Zhang X, Li Y, Ran L, Huang K, He W, Wang R Abstract To investigate the role of dermal Vγ4+γδ T cells in psoriasis-like skin inflammation induced by a re-challenge with imiquimod (IMQ), we compared the development of dermatitis induced by topical application of IMQ in primary challenged mice and re-challenged mice. We also compared the development of dermatitis induced by IMQ between re-challenged control m...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Melatonin represses oral squamous cell carcinoma metastasis by inhibiting tumor-associated neutrophils.
In this study, intensive infiltration of TANs was positively associated with advanced stage, lymphatic metastasis, and poor prognosis of OSCC. Moreover, Mel reduced the survival and migration of OSCC-associated neutrophils. Mechanistically, Mel suppressed the TAN release of C-X-C motif chemokine ligand 8, C-C motif chemokine ligand 2 (CCL2), CCL4, and matrix metalloproteinase-9 by blockage of p38 MAPK and Akt signaling. Mel-fostered TANs decreased the migration and invasion of OSCC cells and reduced tube formation in vitro. Additionally, Mel-hampered pro-motility and pro-angiogenesis effects of TANs were dependent on MMP-9...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Exosomes derived from cardiac telocytes exert positive effects on endothelial cells.
This study aimed to identify cardiac telocytes by their morphological and molecular features and investigate whether their exosomes affect cardiac endothelial cells. To this end, rat cardiac telocytes were cultured and stained with methylene blue, Janus Green B, and MitoTracker green, or with antibodies for established cell surface markers, and examined by microscopy. In addition, telocyte organelles and exosome release were examined by transmission electron microscopy. To investigate exosome functions, we isolated exosomes from telocytes and co-cultured them with endothelial cells in vitro, as well as transfusing them int...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-34a promotes cell cycle arrest and apoptosis and suppresses cell adhesion by targeting DUSP1 in osteosarcoma.
Authors: Gang L, Qun L, Liu WD, Li YS, Xu YZ, Yuan DT Abstract MicroRNAs are often deregulated in most cancer types and have important functions in carcinogenesis and cancer progression. Here, we studied the function of microRNA-34 (miR-34a) in osteosarcoma MG63 and U-2OS cells by expressed with pre-miR-34a, anti-miR-34a and corresponding negative controls, respectively. Cells proliferation, cell cycle and apoptosis was measured by MTT and flow cytometry assay. The effect of miR-34a on DUSP1 expression was evaluated by luciferase assays, real-time PCR and western blot assay. The data showed that miR-34a reduced the...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-346 regulates neural stem cell proliferation and differentiation by targeting KLF4.
In this study, we showed that miR-346, a less characterized microRNA, promoted NSCs proliferation, differentiation and apoptosis by targeting KLF4, a core transcriptional factor in stem cell fate determination. Our data suggested that miR-346 could directly target the 3'-untranslated region of KLF4. Overexpression of miR-346 decreased KLF4 expression at both mRNA and protein levels in NSCs. More importantly, Overexpression of miR-346 repressed NSC proliferation and induced the expression of lineage markers including GFAP and Tuj1. Additionally, overexpression of miR-346 promoted apoptosis of NSCs. In concert, suppressing i...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

1,25-dihydroxyvitamin D3 reduces mouse airway inflammation of neutrophilic asthma by transcriptional modulation of interleukin-17A.
Authors: Qiu YY, Zhou XY, Qian XF, Wu YX, Qin C, Bian T Abstract Corticosteroid resistance and severe airflow obstruction have been proved to participate in the neutrophilic inflammation of airway in uncontrollable asthmatics. IL-17 is one of the pro-inflammatory cytokines produced by Th17 cells, and it plays an important role in the neutrophilic inflammation of airway in steroid-resistant asthmatics. Recent data have proved that 1,25(OH)2D3 represses IL-17A in inflammation and Th17-mediated autoimmunity through vitamin D receptors(VDR) at the level of transcription. Our study validated that 1,25-(OH)2D3 can modula...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Depletion of astrocyte elevated gene-1 suppresses tumorigenesis through inhibition of Akt activity in bladder cancer cells.
Authors: Zhang L, Yang G, Chen H, Huang Y, Xue W, Bo J Abstract Astrocyte elevated gene-1 (AEG-1) has been reported to promote tumorigenesis, however the molecular mechanisms by which AEG-1-induced bladder cancer progression has remained elusive. Here, we identified that depletion of AEG-1 in bladder cancer cells suppressed cell growth. Moreover, we observed that down-regulation of AEG-1 induced apoptosis and inhibited cell migration and invasion. Furthermore, depletion of AEG-1 inhibited Akt activity and suppressed Bcl-2 expression, but upregulated the levels of p21 and p27. Our findings reveal that AEG-1 carries ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

High-intensity resistance training alone or combined with aerobic training improves strength, heart function and collagen in rats with heart failure.
Authors: Alves JP, Nunes RB, Ferreira DDC, Stefani GP, Jaenisch RB, Lago PD Abstract BACKGROUND: Resistance training (RT) has been associated with positive responses in patients with cardiovascular disease, and when it is combined with continuous aerobic training (CAT), favorable adaptations appear to be even more pronounced. However, the effects of high-intensity RT alone or in combined with CAT in the case of heart failure (HF) is not completely elucidated. METHODS: 28 male Wistar rats with HF (90 days old) were allocated to 4 groups: high-intensity RT (RT, n=7), CAT (CAT, n=7), RT and CAT (RT+CAT, n=7) and s...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Gap junctional intercellular communication dysfunction mediates the cognitive impairment induced by cerebral ischemia-reperfusion injury: PI3K/Akt pathway involved.
CONCLUSION: The PI3K/Akt pathway is involved in cognitive impairment caused by gap junctional communication dysfunction in the rat hippocampus after ischemia-reperfusion injury. PMID: 29312496 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Regulation of Kruppel-like factor 2 (KLF2) in the pathogenesis of intracranial aneurysm induced by hemodynamics.
In conclusion, results reveal that KLF2 is up-regulated in the vascular wall of basilar artery, and its overexpression regulates the pathogenesis of IA, which may be a self-protection mechanism of the arterial wall, providing a novel insight for therapy of IA. PMID: 29312497 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

LncRNA SUMO1P3 drives colon cancer growth, metastasis and angiogenesis.
Authors: Zhang LM, Wang P, Liu XM, Zhang YJ Abstract Long noncoding RNA (lncRNA) small ubiquitin-like modifier 1 pseudogene 3 (SUMO1P3) acts a tumor promoter in several malignancies; however, its roles in colon cancer remain unclear. Herein, we demonstrated that SUMO1P3 expression was significantly higher in colon cancer tissues and cell lines than the corresponding non-tumor samples and normal colonic epithelial cells, respectively. The upregulation of SUMO1P3 was positively correlated with the advanced histological stages, metastases, angiogenesis and poor prognosis of colon cancer patients. SUMO1P3 knockdown rep...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD.
Authors: Jing W, Vaziri ND, Nunes A, Suematsu Y, Farzaneh T, Khazaeli M, Moradi H Abstract Progressive deterioration of kidney function in chronic kidney disease (CKD) is mediated by hypertension, oxidative stress, inflammation, and fibrosis. Renin-angiotensin blockade is commonly used to retard CKD progression. In addition, vasoactive peptides have been shown to reduce blood pressure and exert antioxidant, anti-inflammatory and anti-fibrotic effects. We hypothesized that administration of LCZ696 (sacubitril/valsartan) is more effective than valsartan alone in slowing progression of CKD. Male Sprague Dawley rats un...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA).
In this study, we developed an in vitro model in order to characterize the role of endothelial matrix metalloproteinase-9 (e-MMP-9) as a potential trigger of medial disruption and in the inflammatory response bridging between ECs and vSMC. Lentiviral-mediated silencing of e-MMP-9 through RNA interference inhibited TNF-alpha-mediated activation of NF-κB in EA.hy926 human endothelial cells. In addition, EA.hy926 cells void of MMP-9 failed to migrate in a 3D matrix. Moreover, silenced EA.hy926 affected vSMC behavior in terms of matrix remodeling. In fact, also MMP-9 in vSMC resulted inhibited when endothelial MMP-9 was ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The lncRNA-HOXA-AS2/EZH2/LSD1 oncogene complex promotes cell proliferation in pancreatic cancer.
Authors: Lian Y, Li Z, Fan Y, Huang Q, Chen J, Liu W, Xiao C, Xu H Abstract Emerging evidence have indicated that long non-coding RNAs (lncRNAs) play crucial roles in cancer development and progression. Previous studies have suggested that lncRNA-HOXA cluster antisense RNA 2 (HOXA-AS2) is involved in tumorigenesis of several cancers. However, little is known about the alteration and biological functions of HOXA-AS2 in pancreatic cancer (PC). The purpose of this study is to identify the role of HOXA-AS2 in PC. Here, we provided evidence that lncRNA HOXA-AS2 was up-regulated in PC tissues. In addition, Loss-of-functi...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Shikonin induces apoptosis and necroptosis in pancreatic cancer via regulating the expression of RIP1/RIP3 and synergizes the activity of gemcitabine.
Authors: Chen C, Xiao W, Huang L, Yu G, Ni J, Yang L, Wan R, Hu G Abstract Pancreatic cancer is a lethal solid malignancy with poor prognosis. The optimal therapy for patients with advanced pancreatic cancer remains challenged. Thus, development of novel chemotherapy regimens is extremely urgent. Shikonin (SK) is a naphthoquinone derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. It has been considered as effective anti-inflammatory, anti-oxidant, and anti-cancer activity agents in various diseases. In the present study, we found that SK inhibited the growth of human pancreatic cancer ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Biomimetic peptides protect cells from oxidative stress.
Authors: Zhang C, Zhou Y, Yang GY, Li S Abstract Most degenerative diseases are caused by free radicals. Antioxidin-RL peptide is a free radical scavenger found in the skin of plateau frog Odorrana livida, which is more stable than vitamin C as it resists light-induced degradation. However, whether and how antioxidin-RL protects cells from oxidative stress was not clear. Here we addressed this issue, and in addition, we designed a series of antioxidin cognates by adding tyrosine residues to enhance free radical-binding capability. We performed free radical-clearing assays in solution to screen the mutants, and foun...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Cathepsin D protects renal tubular cells from damage induced by high glucose independent of its enzymatic activity.
Authors: Du F, Wang T, Li S, Meng X, Zhang HY, Li DT, Du ZX, Wang HQ Abstract Although glomerular and vascular damage have been considered the main characteristics of diabetic kidney disease (DKD), accumulating data now indicate that tubular atrophy also plays a major role. Cathepsin D (CatD) is the major aspartate protease within lysosomes. The current study demonstrated that CatD expression was altered in the renal tubular epithelium in patients with diabetes mellitus (DM). In contrast to its low and uniform distribution in the tubular epithelium in normal kidney tissues, CatD demonstrated flecked and increased e...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Curcumin inhibits gastric cancer-derived mesenchymal stem cells mediated angiogenesis by regulating NF- κB/VEGF signaling.
Curcumin inhibits gastric cancer-derived mesenchymal stem cells mediated angiogenesis by regulating NF-κB/VEGF signaling. Am J Transl Res. 2017;9(12):5538-5547 Authors: Huang F, Yao Y, Wu J, Liu Q, Zhang J, Pu X, Zhang Q, Xia L Abstract Cancer-derived mesenchymal stem cells (MSCs) seem to play an important role in mediating tumor angiogenesis. Recently, curcumin has been shown to display multiple therapeutic properties, including anticancer activity. In the present study, we have tried to explore the role of curcumin in regulating gastric cancer cells-derived mesenchymal stem cells (GC-MSCs) medi...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

A comparison of apoptosis levels in keloid tissue, physiological scars and normal skin.
In this study, the expression levels of key factors in the mitochondrial pathway and the cell proliferation factor (PCNA) were measured to evaluate the level of apoptosis and proliferation in keloid scars, physiological scars and normal skin tissue. Thirty samples were taken from 30 patients: 10 keloid patients, 10 physiological scar patients and 10 patients without obvious scarring. All 30 patients were selected randomly from the Department of Plastic Surgery at Peking Union Medical College Hospital from June 2016 to December 2016. Hematoxylin and eosin staining and Masson staining were used to observe the differences in ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Metformin inhibits development of diabetic retinopathy through microRNA-497a-5p.
Authors: Zhang Y, Chen F, Wang L Abstract Metformin is an AMP-activated protein kinase activator that is widely prescribed for treating type 2 diabetes. Recently, metformin was reported to slow down the development and alleviate the severity of diabetic retinopathy (DR). However, the underlying mechanisms remain unclear. Here, we used an alloxan-induced diabetes mouse model to study the effects of metformin on the development of DR as well as the mechanisms. We found that DR was induced in alloxan-treated mice 10 weeks after alloxan treatment, and treatment of metformin did not prevent the occurrence of alloxan-ind...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Curcumin combined with glycyrrhetinic acid inhibits the development of hepatocellular carcinoma cells by down-regulating the PTEN/PI3K/AKT signalling pathway.
In this study, curcumin and GA were used separately and in combination to treat human hepatocellular carcinoma (HCC) HepG2 cells. MTT assays were used to evaluate cell proliferation. Flow cytometry was carried out to measure cell apoptosis and determine cell cycle progression. Western blot analyses were applied to determine the expression levels of B-cell lymphoma-2 (Bcl-2), B-cell associated X protein (Bax), phosphatase and tensin homolog (PTEN), phosphorylated phosphoinositide 3-kinase (PI3K) and AKT serine/threonine kinase 1 (Akt). The results showed that combined treatment with curcumin and GA resulted in a significant...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-423 enhances the invasiveness of hepatocellular carcinoma via regulation of BRMS1.
This study sheds light on miR-423 as a crucial factor that enhances HCC cell invasiveness, and suggests miR-423 as a promising therapeutic target for HCC treatment. PMID: 29312509 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Qiliqiangxin attenuates isoproterenol-induced cardiac remodeling in mice.
CONCLUSIONS: Our study demonstrated that QL treatment provided cardioprotection against ISO-induced cardiac remodeling by improving PPARγ level, which could be as the potential therapeutic target in reversing cardiac remodeling and heart failure. PMID: 29312510 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

The long non-coding RNA SUMO1P3 facilitates breast cancer progression by negatively regulating miR-320a.
In this study, we showed that SUMO1P3 expression was higher in breast cancer tissues when compared to adjacent normal tissues and we found that high levels of SUMO1P3 expression associated significantly with tumor progression and poor survival of breast cancer patients. Moreover, we found that knockdown of SUMO1P3 suppressed proliferation, migration, and invasion of breast cancer cells. Bioinformatics analysis and luciferase reporter assays confirmed that SUMO1P3 binds to miR-320a, which has been identified as a tumor suppressor in various cancers, including breast cancer. We also confirmed that the tumor-promoting effects...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Rabbit model of subchondral bone bruise and the treatment potential of calcitonin.
CONCLUSION: Free fall counterpoise is a promising method to establish a subchondral bone bruise model in rabbits. Calcitonin injection is a potential treatment for subchondral bone bruise lesions. PMID: 29312512 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

NLRP3-inflammasomes are triggered by age-related hearing loss in the inner ear of mice.
In conclusion, inflammation triggered by the activation of inflammasomes in the cochleae of aging mice appears to be playing an important role in the pathological process of ARHL and may be a potential cause of presbyacusis. PMID: 29312513 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Deletion of diacylglycerol-responsive TRPC genes attenuates diabetic nephropathy by inhibiting activation of the TGF β1 signaling pathway.
In conclusion, deletion of DAG-responsive TRPCs attenuates diabetic renal injury via inhibiting the upregulation of TGFβ1 signaling in diabetic kidneys. PMID: 29312514 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Chidamide shows synergistic cytotoxicity with cytarabine via inducing G0/G1 arrest and apoptosis in myelodysplastic syndromes.
In this study, the combination of chidamide (50 nM) with cytarabine (50 nM) showed synergistic inhibition on cell growth.The mean combination index values were 0.068, 0.158, and 0.226 in SKM-1, MUTZ-1, and KG-1 MDS cell lines, respectively. The combination increased the acetylation levels of histone H3 and decreased HDAC activity in MDS cells.A low concentration (25 and 50 nM) of chidamide combined with low-dose cytarabine (50 nM) inhibited cell proliferation and arrested the cell cycle in the G0/G1 phasevia down-regulating CDK2 and up-regulating p21. Furthermore, the combined treatment induced cell apoptosis via down-regu...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Salvianolic acid A attenuates early brain injury after subarachnoid hemorrhage in rats by regulating ERK/P38/Nrf2 signaling.
Authors: Gu X, Zheng C, Zheng Q, Chen S, Li W, Shang Z, Zhang H Abstract Oxidative stress and inflammation play an important role in the pathogenesis of early brain injury (EBI) following subarachnoid hemorrhage (SAH). The present study aimed to evaluate the effect of salvianolic acid A (SalA) on EBI after SAH via its antioxidative, anti-inflammatory, and anti-apoptotic effects. The intraperitoneal administration of SalA (10 and 50 mg/kg/day) significantly alleviated EBI (including neurobehavioral deficits, brain edema, blood-brain barrier permeability, and cortical neuron apoptosis) after SAH in rats. SalA treatme...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Increased miR-155 and heme oxygenase-1 expression is involved in the protective effects of formononetin in traumatic brain injury in rats.
In this study, the neuroprotective and antioxidant effects of formononetin against TBI, as well as the related probable mechanisms, were investigated. The TBI model was produced in male Wistar rats through Feeney's weight-drop model. At 1 day after TBI, the neurological function score and brain water content were assessed. TUNEL assay was used to determine neuronal apoptosis. The expression levels of miR-155, HO-1, and BACH1 were measured by RT-PCR and western blotting. Consequently, our findings showed that formononetin pretreatment for 5 days significantly improved the neurological scores, reduced brain edema and inhib...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Morin, a plant derived flavonoid, modulates the expression of peroxisome proliferator-activated receptor- γ coactivator-1α mediated by AMPK pathway in hepatic stellate cells.
Morin, a plant derived flavonoid, modulates the expression of peroxisome proliferator-activated receptor-γ coactivator-1α mediated by AMPK pathway in hepatic stellate cells. Am J Transl Res. 2017;9(12):5662-5670 Authors: Yuan W, Ahmad S, Najar A Abstract Morin exerts inhibitory effects on hepatic stellate cell (HSC) stimulation which is considered important step for fibrogenesis in liver. These morin-induced inhibitory effects are mediated through enhancement in the expression levels of peroxisome proliferator-activated receptor-γ (PPARγ). PPARγ plays a critical role in in...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Constitutive activation of STAT3 and cyclin D1 overexpression contribute to proliferation, migration and invasion in gastric cancer cells.
Authors: Luo J, Yan R, He X, He J Abstract Signal transducer and activator of transcription-3 (STAT3) is closely associated with tumorigenesis and is activated in tumor cells of a variety of cancers. The raised expression of cyclin D1 (CCND1) by activated STAT3 has been verified in some cancers. However, the relationship between STAT3 and CCND1 has yet to be studied in gastric cancer (GC) cells. In the present study, we found that STAT3 was constitutively activated in several GC cells together with overexpressed CCND1. In addition, IL-6 treatment enhanced the expression level of p-STAT3 and CCND1, accelerating the ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Fisetin suppresses malignant proliferation in human oral squamous cell carcinoma through inhibition of Met/Src signaling pathways.
Authors: Li YS, Qin XJ, Dai W Abstract Fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary flavonoid and has been indicated as a novel anti-cancer agent in several types of cancer cells. However, the mechanisms underlying the effect of fisetin in human oral squamous cell carcinoma (OSCC) remain unclear. Here, we report that fisetin significantly inhibits tumor cell proliferation and induces apoptosis in OSCC (UM-SCC-23 and Tca-8113) cancer cell lines. Further analysis demonstrates that fisetin also inhibits Met/Src signaling pathways using the PathScan® receptor tyrosine kinases (RTK) Signaling Antibody Array ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Predictive and prognostic value of sphingosine kinase 1 expression in patients with invasive ductal carcinoma of the breast.
Authors: Do SI, Kim HS, Kim K, Lee H, Do IG, Kim DH, Chae SW, Sohn JH Abstract Sphingosine kinase 1 (SPHK1) has been found to be upregulated in many different types of human malignancy and plays a crucial role in cancer development and progression. However, the potential of SPHK1 to act as a predictive and prognostic biomarker in breast cancer remains to be clarified. In the present study, SPHK1 expression was evaluated in breast cancer cell lines and 224 breast cancer tissue samples using immunohistochemical staining. Compared to the normal mammary epithelial cell line MCF-10A, SPHK1 mRNA and protein expression le...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model.
CONCLUSION: LincRNAs were identified differently between the BPD model and the normoxia group. Many target genes were involved in the developmental process, including cell component biogenesis, biological regulation, transcription regulator, and translation regulator. The BPD might be caused by the activation of the pathways of the EMC-receptor interaction, cytokine-cytokine receptor interaction, cell cycle, and cell adhesion molecules. The present study provides new insight into the pathogenesis mechanism of BPD. PMID: 29312522 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells.
In this study, we examined the expression of C4orf7 in PDL tissue, using immunohistochemical staining. We transfected PDLCs with lentiviral vectors expressing C4orf7 and examined the effect of C4orf7 on the balance of PDLC osteogenic and osteoclastogenic differentiation. Osteogenic induction resulted in the downregulation of mRNA and protein expression levels of the osteogenic/cementoblastic markers: ALP, RUNX2, COL1, OPN, OPG, OSX, IBSP, CAP, and CEMP1. Transfected cells also exhibited an increased RANKL/OPG ratio, which is an indicator of osteoclastogenic differentiation. ALP activity assays and Alizarin red staining con...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

Research on ribosome-inactivating proteins from angiospermae to gymnospermae and cryptogamia.
Authors: Liu WY Abstract Ribosome-inactivating Proteins (RIPs) are a group of cytotoxin proteins that usually contain a RNA N-glycosidase domain, which irreversibly inactivates ribosome, thus inhibiting protein synthesis. During the past 14 years (1990-2004), the studies conducted in our laboratory had been focusing on the structure and enzymatic mechanism of several PIPs. Herein, we briefly described a summary of the studies conducted mainly in our laboratory on RIPs from angiospermae to gymnospermae and cryptogamia as follows. (1) Cinnamomin is a novel type II RIP isolated from mature seeds of camphor tree. Like ...
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MiR-30a attenuates osteoclastogenesis via targeting DC-STAMP-c-Fos-NFATc1 signaling.
In conclusion, miR-30a attenuated osteoclastogenesis via suppression of DC-STAMP-c-Fos-NFATc1 signaling pathway. On these grounds, this study may reveal a new promising target for the treatment of osteoporosis and other osteopenic disorders. PMID: 29312525 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - January 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research