The pathogenesis and treatment in antineutrophil cytoplasmic antibody associated vasculitis.
Authors: Li SS, Wang SL, Zhang HJ, He SH, Liang X, Li TF Abstract Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV) is a systemic autoimmune disease characterized by leukocytoclastic inflammation of small blood vessels. Commonly detected autoantibodies include anti-protease 3 (PR3) and anti-myeloperoxidase (MPO). Although cell necrosis plays an important role in the production of autoantibodies and the pathogenesis of AAV, the correlation between their titers and disease activity remains elusive. As improved detection techniques facilitate early diagnosis, a satisfactory efficacy can be achiev...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Identification and validation of novel metastasis-related signatures of clear cell renal cell carcinoma using gene expression databases.
Authors: Ma CG, Xu WH, Xu Y, Wang J, Liu WR, Cao DL, Wang HK, Shi GH, Zhu YP, Qu YY, Zhang HL, Ye DW Abstract Patients with clear cell renal cell carcinoma (ccRCC) typically face aggressive disease progression when metastasis occurs. Here, we screened and identified differentially expressed genes in three microarray datasets from the Gene Expression Omnibus database. We identified 112 differentially expressed genes with functional enrichment as candidate prognostic biomarkers. Lasso Cox regression suggested 10 significant oncogenic hub genes involved in earlier recurrence and poor prognosis of ccRCC. Receiver opera...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effects of NP-1 on proliferation, migration, and apoptosis of Schwann cell line RSC96 through the NF- κB signaling pathway.
Effects of NP-1 on proliferation, migration, and apoptosis of Schwann cell line RSC96 through the NF-κB signaling pathway. Am J Transl Res. 2020;12(8):4127-4140 Authors: Kou Y, Yu F, Yuan Y, Niu S, Han N, Zhang Y, Yin X, Xu H, Jiang B Abstract Peripheral nerve injury is a common refractory disease in the clinic that often leads to dysfunction of movement and sensation. Different from other tissue injuries, peripheral nerve injury needs a longer time for regeneration. Therefore, effective drug therapy is needed to promote nerve regeneration in the treatment of peripheral nerve injury. Our prelimin...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Zinc finger and BTB domain-containing 7C (ZBTB7C) expression as an independent prognostic factor for colorectal cancer and its relevant molecular mechanisms.
Authors: Chen X, Jiang Z, Pu Y, Jiang X, Xiang L, Jiang Z Abstract Currently, colorectal cancer (CRC) predictions are based on an early diagnosis and the tumor-node-metastasis (TNM) stage, but the outcomes of patients with the same cancer type are difficult to predict. Novel molecular tests for the early diagnosis and stratification of CRC patients must be devised. After our initial bioinformatics screen, we examined zinc finger and BTB domain-containing 7C (ZBTB7C). To date, few studies have investigated ZBTB7C in CRC, necessitating further analyses of its expression and regulatory mechanism in CRC. ZBTB7C mRNA an...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Intracerebral hemorrhage alters circular RNA expression profiles in the rat brain.
Authors: Zhong Y, Li X, Li C, Li Y, He Y, Li F, Ling L Abstract Circular RNAs (circRNAs), formed from pre-messenger RNAs by back-splicing, are a novel class of evolutionarily-conserved endogenous non-coding RNAs. While circRNAs are involved in various diseases, the role of circRNAs in intracerebral hemorrhage (ICH) remains unknown. In the present study, we performed high-throughput sequencing to profile the expression of circRNAs in the rat brain at 24 and 48 hours after ICH onset, and utilized bioinformatics methods to make predictions about the function of dysregulated circRNAs. Compared with the sham group, 346 ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Targeted p21 activation by a new double stranded RNA suppresses human prostate cancer cells growth and metastasis.
Authors: Zhang Q, Yang X, Luo L, Ma X, Jiao W, Li B, Zhang M, Zhao K, Niu H Abstract We have previously demonstrated that miR-1236-3p and its sequence homology dsRNA, dsRNA-245 (which is completely complementary to the p21 promoter) had potential ability to upregulate p21 expression by targeting specific promoter sequence and inhibited bladder cancer (BCa). However, we still know little about the effect of miR-1236-3p on prostate cancer and which dsRNA has an inhibitory effect on prostate cancer (PCa)? Here, we confirmed that miR-1236-3p was decreased in PCa cells and tissues. MiR-1236-3p inhibited PCa cells growth...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

An injectable liposome for sustained release of tanshinone IIA to the treatment of acute blunt muscle injury by augmenting autophagy and alleviating oxidative stress.
In this study, tanshinone IIA liposomes were prepared to improve the bioavailability and sustained release of tanshinone IIA. The particle size, dispersion coefficient, zeta potential, encapsulation efficiency (EE) and drug loading (DL) of tanshinone IIA liposomes were 150.67 ± 27.23 nm, 0.20 ± 0.015, -8.73 ± 2.28 mV, 70.32 ± 4.04% and 15.63%, respectively. The results of quantitative real-time polymerase chain reaction (QRT-PCR) showed that tanshinone IIA liposome significantly promoted the expression of vimentin and reduce MHCIIB expression compared with other groups (P
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Long noncoding RNA lysophospholipase-like 1-2 as ceRNA modulates glioma metastasis by regulating miR-217/YWHAG.
CONCLUSIONS: LncRNA-LYPLAL1-2 suppressed glioma metastasis via the miR-217/YWHAG axis and is expected to be a potential target for early diagnosis and treatment of gliomas. PMID: 32913498 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Cell-free fat extract accelerates diabetic wound healing in db/db mice.
Authors: Wang X, Deng M, Yu Z, Cai Y, Liu W, Zhou G, Wang X, Cao Y, Li W, Zhang W Abstract Cell-free fat extract (CEFFE), the liquid fraction derived from fat tissues, is enriched with a variety of growth factors and possesses pro-angiogenic, anti-apoptotic, and anti-oxidative properties. The aim of this study was to determine if CEFFE could accelerate chronic wound healing in mice with diabetes and investigate its underlying mechanisms. A model of circular full-thickness wound (6 mm diameter) was produced in the central dorsal region of spontaneous type 2 diabetes mellitus db/db mice. The mice were divided to thre...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Rapid and fully-automated detection of Clostridium difficile Toxin B via magnetic-particle-based chemiluminescent immunoassay.
Authors: Qi H, Wang Y, Wu P, Ma Y, Wang J Abstract Clostridium difficile colitis is caused by a cytotoxin produced by the anaerobic bacteria C. difficile on the epithelial cells of the large intestine, particularly C. difficile toxin B (Tcd B). Current C. difficile toxin assays have proven to be insensitive and have thus been ruled out from diagnostic purposes. Therefore, Tcd B detection via sandwich-type chemiluminescent immunoassay was proposed as a straightforward approach with potential diagnostic applicability. Here, two high-affinity anti-Tcd B monoclonal antibodies were successfully identified and implemente...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Proteomic screening identifies PML/p53 axis as a potential treatment target of facial nerve schwannomas.
Authors: Wang Z, Chen H, Xue L, He W, Jiang L, Wang Z, Wu H Abstract Facial nerve schwannomas (FNS) represents one of the more difficult treatment paradigms in neurotology. The aim of this study is to investigate the molecular alterations of FNS, thus providing potential targets treatable in the tumour. We for the first time suggest that the deficiency of merlin (the product of NF2 tumour suppressor) is probably one of the key mechanisms underlying FNS tumourigenesis, although no disease-causing NF2 mutations were demonstrated in tumour samples. TMT-labeled spectrometry analysis was used to identify the proteome of...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA expression profiling in an ovariectomized rat model of postmenopausal osteoporosis before and after estrogen treatment.
Authors: Xu X, Zhang P, Li X, Liang Y, Ouyang K, Xiong J, Wang D, Duan L Abstract Postmenopausal osteoporosis (PMOP) is a common disease that seriously threatens human health. Estrogen deficiency plays an essential role in the pathogenesis of PMOP. MicroRNAs (miRNAs) are involved in the development and progression of PMOP. Therefore, identification of miRNAs in PMOP due to estrogen deficiency may contribute to earlier diagnosis and better treatment of this disease. The rat model of PMOP was established by ovariectomy. After one month of treatment, the knee joints were evaluated by microcomputed tomography and histo...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

SPR064, a pro-drug of paclitaxel, has anti-tumorigenic effects in endometrial cancer cell lines and mouse models.
Authors: Zhao X, Kong W, Tucker K, Staley A, Fan Y, Sun W, Yin Y, Huang Y, Fang Z, Wang J, Sen S, Dugar S, Zhou C, Bae-Jump VL Abstract Paclitaxel is one of the most effective and widely used agents in treating a variety of cancers, including endometrial cancer. Because of its poor solubility in water, the current intravenous pharmaceutical paclitaxel is formulated in Cremophor EL and dehydrated in ethanol in equal volumes. Cremophor EL is capable of causing complement activation, which can trigger an immediate hypersensitivity reaction. SPR064 is a pro-drug of paclitaxel which has a much higher solubility as compa...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Silencing miR-454 suppresses cell proliferation, migration and invasion via directly targeting MECP2 in renal cell carcinoma.
Authors: Liu H, Liu QL, Zhai TS, Lu J, Dong YZ, Xu YF Abstract Renal cell cancer (RCC) is one of the most common malignant tumors of the urinary system. MicroRNA-454 (miR-454) has been reported to play an important role in various cancer progressions, such as hepatocellular carcinoma, breast cancer and glioblastoma. Nevertheless, its effect on RCC still remains unknown. We aimed to investigate the biological function and underlying mechanisms of miR-454 in RCC. The expressions of miR-454 and MECP2 in RCC tissues were assessed using data from TCGA database and our own clinical samples. Functional experiments Cell Co...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

TMT-based proteomic analysis reveals the effects of chloroquine on human podocytes.
Authors: Kang Y, Law HK, Zhang Y, Wu Y, Zhu GH, Saleem MA, Huang WY Abstract Chloroquine (CQ) is an antimalarial drug widely used in rheumatic, immunological and infectious diseases. CQ is also a well-known autophagy inhibitor. It slows the progression of renal injury in patients with rheumatology diseases. Long-term CQ treatment could also damage podocytes which are highly differentiated cells wrapping the glomerular capillary to maintain renal filtration. However, the related underlying mechanism remains unclear. The effects of CQ treatment on podocytes need to be elucidated. Our results showed that CQ diminished...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Long non-coding RNA LINC01116 is overexpressed in lung adenocarcinoma and promotes tumor proliferation and metastasis.
Authors: Zeng L, Lyu X, Yuan J, Wang W, Zhao N, Liu B, Sun R, Meng X, Yang S Abstract Long non-coding RNA LINC01116 is involved in the occurrence and progression of a variety of cancers. However, the specific role of LINC01116 in lung adenocarcinoma (LUAD) remains unclear. In this work, we found that LINC01116 was overexpressed in LUAD tissues and cell lines and that increased expression was significantly associated with worse prognoses in patients with LUAD. Univariate and multivariate Cox regression analyses indicated that LINC01116 was an independent risk factor for the prognosis of patients with LUAD. Downregul...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

CoQ10 suppression of oxidative stress and cell senescence increases bone mass in orchiectomized mice.
Authors: Wu X, Liang S, Zhu X, Wu X, Dong Z Abstract Numerous studies support the detrimental effects of oxidative stress and cell senescence on skeletal homeostasis. Coenzyme Q10 (CoQ10) acts as a scavenger for oxidative stress and protects mitochondrial activity from oxidative damage. However, it is unclear whether CoQ10 has a protective effect on osteoporosis caused by orchiectomy. To investigate suppression effect of antioxidant CoQ10 on osteoporosis in orchiectomized (ORX) mice, ORX mice were supplemented with/without CoQ10, and were compared with each other and with sham-operated mice. Our results showed that...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Ulinastatin attenuates monocyte-endothelial adhesion via inhibiting ROS transfer between the neighboring vascular endothelial cells mediated by Cx43.
Authors: Ding H, Jiang Y, Jiang Y, Yuan D, Xiao L Abstract Ulinastatin is widely used in the treatment of pancreatitis and sepsis, because of its excellent anti-inflammatory and antioxidant effects. However, its effects on atherosclerosis, an inflammatory vascular disease, are rarely reported. Therefore, in present study, we explored effects of ulinastatin on monocyte-endothelial adhesion, the initiator of atherosclerosis. We used U937 monocytes and angiotensin II-stimulated human umbilical vein endothelial cells (HUVECs) to build the model of monocyte-endothelial adhesion. Different methods were used to change the...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Overexpression of lncRNA Gm43050 alleviates apoptosis and inflammation response induced by sevoflurane treatment by regulating miR-640/ZFP91.
CONCLUSION: We provided comprehensive data of the function and mechanism of lncRNA Gm43050 in abnormal cognition. Our study showed that lncRNA Gm43050 exerted its important role via the regulation of miR-640 and ZFP91. PMID: 32913509 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Association between acute severe mercury poisoning and multiple organ failure.
Authors: Bai L, Peng X, Liu Y, Sun Y, Zheng L, Liu Z, Wan K, Wang J, Zhao J, Qiu Z Abstract Acute mercury poisoning, involving a number of organs, leads to severe dysfunctions, such as acute renal failure (ARF), and even threatens patients' lives. A case of acute severe mercuric chloride (HgCl2) poisoning with multiple organ failure was reported in this study. A 38-year-old woman orally took about 50 g HgCl2 powder in 2015, and showed nausea, emesis, clouding of consciousness, lip and nail cyanosis, and dark red bloody fluid from bilateral nostrils. Based on chest and abdominal CT examinations, gastroscopy, and col...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Rho-kinase inhibition by Fasudil promotes tumor maturation and apoptosis in small-cell lung cancer.
In conclusion, these results indicated a potential and novel application of Fasudil for SCLC treatment. PMID: 32913511 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The etiological role of endoplasmic reticulum stress in acute lung injury-related right ventricular dysfunction in a rat model.
This study aimed to ascertain whether endoplasmic reticulum (ER) stress participates in acute lung injury (ALI) and related right ventricular dysfunction (RVD) as well as to explore the underlying mechanisms of these conditions. A single intratracheal instillation of lipopolysaccharide (LPS) (10 mg/kg) was used to establish the RVD model. The ER stress inhibitor, 4-PBA (500 mg/kg), was administered using a gavage 2 hours before and after the LPS treatment for prevention and treatment, respectively. At 12 hours post-LPS exposure, mRNA and protein expressions of ER stress-specific biomarkers, glucose regulating protein 78 (G...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Telocytes enhanced in vitro decidualization and mesenchymal-epithelial transition in endometrial stromal cells via Wnt/ β-catenin signaling pathway.
This study further investigates the hypothesis that TCs, a source of Wnt, modulates decidualization and MET in ESCs. We had observed differential expression of Wnt ligands in primary mice ESCs and TCs by qPCR. TCM-induced decidualization and MET was assessed in ESCs. Changes in markers for decidualization (cyclin-D3, desmin, d/tPRP), stromal cells (N-cadherin), epithelial cells (E-cadherin), and the Wnt/β-catenin pathway (β-catenin, FOXO1) were quantified by western blot and RT-PCR. β-catenin knockdown in ESCs decreased the degree of TCM-induced decidualization and MET, with significantly reversed expression profiles (P
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Suppression of estrogen receptor-beta promotes gastric cancer cell apoptosis with induction of autophagy.
In conclusion, these results demonstrated that suppression of ESR2 gene expression could promote GC cell apoptosis, suggesting that it may prove to be a potential therapeutic target for GC. PMID: 32913514 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Reduced white matter integrity associated with cognitive deficits in patients with drug-naive first-episode schizophrenia revealed by diffusion tensor imaging.
In this study, we investigated the possible associations between white matter integrity and cognitive deficits in drug-naive first-episode schizophrenia (dn-FES) using diffusion tensor imaging (DTI). A total of 96 participants, including 46 dn-FES patients and 50 healthy individuals, underwent 3.0 T magnetic resonance diffusion-weighted imaging and cognitive assessments using the Chinese version of the Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery (MCCB). Group differences were tested using tract-based spatial statistics (TBSS). Compared with the control grou...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

LncRNA RP11-395G23.3 suppresses the endometrial cancer progression via regulating microRNA-205-5p/PTEN axis.
Authors: Xin W, Gao X, Zhao S, Zhao P, Yu H, Wu Q, Hua K Abstract The focal point of this research was the functional role of RP11-395G23.3 in endometrial cancer (EC). The expression of RP11-395G23.3, microRNA (miRNA)-205-5p, and their target proteins were detected by quantitative real-time polymerase chain reaction and western-blot analyses. Flow cytometry and proliferation, Transwell, and wound healing assays were used to detect the effects of RP11-395G23.3 and miRNA-205-5p on tumor cell migration and proliferation in vitro. RP11-395G23.3 expression was negatively related to miRNA-205-5p, but positively related t...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Pathogenic role of circulating CD4+CXCR5+ cell subpopulations in patients with chronic spontaneous urticarial.
Authors: Gao C, Chen WC, Liu W, Chen Q, Chen S, Xu Y, Song Z Abstract Accumulating evidence demonstrated that circulating CD4+CXCR5+, follicular helper T (Tfh) and follicular regulatory T (Tfr) cells maintain immune homeostasis and humoral immune response and were involved in the pathogenesis of certain autoimmune/inflammatory diseases. The current study was aimed to investigate the correlation between frequencies of CD4+CXCR5+, Tfh and Tfr cells, Tfh/Tfr and the disease activity in chronic spontaneous urticaria (CSU). Frequencies of CD4+CXCR5+, Tfh and Tfh/Tfr, but not Tfr, in peripheral blood mononuclear cells (P...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

KLF14 inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells by downregulating WNT3A.
In conclusion, we describe a new mechanism for KLF14 in differentiation of hMSCs into osteoblasts and suggest a new target for clinical therapeutics related to human bone development. PMID: 32913518 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

SNHG9 was upregulated in NSCLC and associated with DDP-resistance and poor prognosis of NSCLC patients.
Authors: Wang R, Chen C, Kang W, Meng G Abstract Lung cancer, a leading cause of cancer-related mortalities worldwide and non-small cell lung cancer (NSCLC) is the main subtype of lung cancer. As a first-line chemotherapeutic drug used for NSCLC, acquired resistance retarded the clinical application of cisplatin (DDP). We herein reported long non-coding RNA SNHG9 was over-expressed in NSCLC tissues and cell lines compared with normal lung tissues and cell line; Increased SNHG9 was also observed in DDP resistant NSCLC tissues and cell lines compared with their DDP sensitive counterparts. Elevated expression of SNHG9...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Elabela alleviates myocardial ischemia reperfusion-induced apoptosis, fibrosis and mitochondrial dysfunction through PI3K/AKT signaling.
In this study, the rat I/R model was established in vivo. Following treatment with Elabela, the histopathological changes of heart tissue were evaluated by the hematoxylin and eosin- or Masson's trichrome staining. Apoptosis of heart tissue was examined using TUNEL staining. The expression of type I or III collagen and apoptosis-associated proteins was measured using western blotting. Moreover, myocardial ultrastructure in myocardium was detected via electron microscopy analysis. H9c2 cells were treated with hypoxia/reoxygenation (H/R) to mimic the myocardial I/R injury in vitro. After treatment with Elabela or Elabela com...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The FOXM1/ATX signaling contributes to pancreatic cancer development.
In conclusion, the present work identified a positive feedback loop between ATX and FOXM1 which promotes PDAC cell proliferation and migration. PMID: 32913521 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effect of AAV9-hIGF-1 on inflammatory reaction in mdx mice and its mechanism.
This study aimed to the role of insulin-like growth factor 1 (IGF-1) in Duchenne muscular dystrophy (DMD), the inflammatory response and the potential mechanism of the effect hIGF1 exerted in muscle inflammation were also been explored. In this study, AAV9, a carrier of the human IGF-1 gene, was injected into mdx mice to observe the role of IGF-1 in DMD. Routine histopathological staining, immunofluorescence and western blot were used to detect the inflammatory response. In addition, we also explored the potential mechanism of the role of hIGF1 in muscle inflammation. The expression of AAV9 in myocardium and muscle tissue ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Hemangioblastoma: clinicopathologic study of 42 cases with emphasis on TFE3 expression.
Authors: Yang Y, Gao H, Zhen T, Tuo Y, Chen S, Liang J, Han A Abstract Hemangioblastomas (HBs) histologically overlap with TFE3 rearrangement-associated tumors, which present as alveolar architecture and clear or eosinophilic granular cytoplasm. However, whether TFE3 is expressed in HBs remains unexplored. Herein, we analyzed the clinicopathologic features of 42 HBs emphasizing studies of TFE3 expression. Of 42 cases, 38 were sporadic and 4 were regarded as a part of von Hippel-Lindau (VHL) syndrome according to clinical presentation. Nineteen patients were male and 23 were female. Patient age ranged from 17 to 70 ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Moringa oleifera seeds mitigate myocardial injury and prevent ventricular failure induced by myocardial infarction.
In this study, we found MOI seeds could improve the 28-day survival rate and the cardiac functions of myocardial infarction (MI) mice, with significantly increased ejection fraction and fractional shortening by day 28 post-MI. Correspondingly, the infarctional areas of heart were markedly decreased. Mechanistically, MOI seeds inhibited MI-induced apoptosis and repressed the degree of cardiac fibrosis. Further mechanistic studies indicated cardioprotective the effects of MOI seeds mainly via the suppression of oxidative and nitrosative stress. Taken together, our work suggested a beneficial role of MOI seeds in MI-induced m...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

EphB4 signaling maintains the contractile phenotype of adult venous smooth muscle cells.
CONCLUSIONS: EphB4 was present and functional in adult human venous SMCs. Stimulation of EphB4 increased expression of contractile SMC phenotypic markers and decreased SMC migration in vitro, functioning to retain the contractile phenotype of SMCs. EphB4 activation, therefore, recapitulates changes observed during vein graft adaptation to the arterial environment in vivo. EphB4 represents a new strategy to inhibit neointimal hyperplasia during vein graft adaption. PMID: 32913525 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

MRI predictors of intracranial hemorrhage in acute ischemic stroke after endovascular thrombectomy therapy.
CONCLUSIONS: DWI volume, PWI volume and>3 passes with the retriever were able to predict the ICH in patients with AIS after EVT therapy. PMID: 32913526 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

ALKBH5 promotes colon cancer progression by decreasing methylation of the lncRNA NEAT1.
Authors: Guo T, Liu DF, Peng SH, Xu AM Abstract Colon cancer is the third leading cause of cancer-related deaths all around the world. LncRNA methylation has been verified to participate in some kinds of malignancies. The aim of this study was to investigate the function of NEAT1 in colon cancer and further explore the potential mechanism between NEAT1 and ALKBH5. Differential expression of lncRNAs between colon cancer tissues and normal tissues was identified using ArrayStar lncRNA microarrays. The levels of NEAT1 and ALKBH5 expression in colon cancer tissues and cells were measured using qRT-PCR. MTT, transwell m...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The effect of traditional Tibetan guozhuang dance on vascular health in elderly individuals living at high altitudes.
Authors: Li G, Zhu A, Huang Y, Meng J, Ji L, Xue J, Li H, Wang X, Luo J, Wu Z, Wu S Abstract To evaluate the effect of dance on vascular-related factors and cerebral hemodynamics in elderly individuals in Qinghai-Tibetan plateau regions (mean altitude ≥2,300 m). Thirty elderly individuals, who practiced traditional Tibetan Guozhuang dance or did not, were enrolled, respectively. Serum PGC-1α, HCY, FSTL-1, VEGF and HIF-1α were measured by ELISA assays. Carotid artery stenosis and plaque, IMT, extracranial internal carotid artery stenosis and cerebral arteriosclerosis were evaluated using CUS and TCD. ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Fluorescence in situ hybridization for WWTR1-CAMTA1 has higher sensitivity and specificity for epithelioid hemangioendothelioma diagnosis.
Authors: Yang P, Zhang S, Yu C, Yan W, Yang N, Li N, Huang Y, Chen H, Cai Z, Zhao Y, Qin Y, Gong X, Feng Z Abstract Epithelioid hemangioendothelioma (EHE) is a rare medium-to-low-grade malignant vascular tumor characterized by vascular differentiation along with specific morphological and genetic alterations. Approximately 90% and 5% of EHE cases are associated with the WWTR1-CAMTA1 and YAP1/TFE3 fusion gene, respectively. Therefore, nuclear CAMTA1 protein expression is considered to be an effective marker for EHE diagnosis. However, the specificity and reliability of this approach have recently been put into quest...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The predictive value of serum amyloid A and C-reactive protein levels for the severity of coronavirus disease 2019.
This study was designed to assess the levels of human serum amyloid A (SAA) and C-reactive protein (CRP) in patients with coronavirus disease 2019 (COVID-19) to determine their prognostic value in predicting the severity of disease. Patients with COVID-19 who presented with acute respiratory distress syndrome (ARDS) shared distinct characteristics. For example, the patients were older, and had higher levels of inflammatory indicators [i.e., levels of CRP, SAA, procalcitonin (PCT), and interleukin-6; CRP-to-PCT ratio; SAA-to-CRP ratio; and neutrophil-to-lymphocyte ratio (NLR)], higher inflammatory cell counts (i.e., white b...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Use of whole-exome sequencing to identify a novel ADCY10 mutation in a patient with nephrolithiasis.
In this study, employing total exon sequencing technology, we investigated two patients in south-central China with primary nephrolithiasis and identified a novel ADCY10 mutation c.2186G> A (p.G729E) and a known ADCY10 mutation c.2182G> A (p.E728K). The results of our study suggest that ADCY10 plays an important role in nephrolithiasis. PMID: 32913531 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effects of aerobic and strength training on aerobic capacity, muscle strength, and gene expression of lymphomonocytes in patients with stable CAD.
This study examined the effectiveness, suitability, and safety of a mixed interval-type aerobic and strength training program (MIAST) on physical fitness in patients with stable coronary artery disease (CAD) without history of myocardial infarction (MI). Twenty-three patients with stable CAD were randomly assigned to a MIAST (n = 12; mean age 58.6 years) or control (n = 11; 63.3 years) group. The MIAST group participated in the progressive training program twice a week for 21 weeks. Peak oxygen uptake (VO2peak), workload, and exercise time were measured as were maximal muscle strength, serum lipids, glucose concentration, ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

The efficacy and safety of remimazolam tosylate versus propofol in patients undergoing colonoscopy: a multicentered, randomized, positive-controlled, phase III clinical trial.
This study aimed to evaluate the efficacy and safety of remimazolam tosylate versus propofol in patients undergoing colonoscopy. In this multicentered, blinded, randomized, active-controlled, non-inferior phase III trial, 384 eligible patients who were about to undergo colonoscopy were randomized as a ratio of 1:1 into remimazolam and propofol group. Procedure success was assessed and defined as the completion of colonoscopy without administration of rescue sedative agent or more than 5 top-ups of trial drug in any 15 minute-period after initial administration of trial drug. Sedation quality was evaluated by Modified Obser...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Comparison in efficacy and safety of forceps biopsy for peripheral lung lesions guided by endobronchial ultrasound-guided sheath (EBUS-GS) and electromagnetic navigation bronchoscopy combined with EBUS (ENB-EBUS).
This study retrospectively summarized the case data of patients who underwent EBUS-GS or ENB-EBUS, both procedures performed at the respiratory endoscopy center of Tangdu Hospital, and the study compared the diagnostic efficacy and complications of the two methods. The study included 93 patients who underwent EBUS-GS and 26 who underwent ENB-EBUS. The diagnostic rates of EBUS-GS and ENB-EBUS were 71.1% and 65.4%, respectively, with no statistical difference (P=0.581). Furthermore, 89.2% of patients in the EBUS-GS group were diagnosed with malignant disease, which was significantly higher than 23.5% diagnosed with malignant...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Early intramyocardial implantation of exogenous mitochondria effectively preserved left ventricular function in doxorubicin-induced dilated cardiomyopathy rat.
This study tested the hypothesis that early implantation of mitochondria (Mito) into left myocardium could effectively protect heart against doxorubicin/12 mg/kg-induced dilated cardiomyopathy (DCM) in rat. Adult-male SD rats (n = 18) were equally categorized into group 1 (sham control), group 2 (DCM) and group 3 [DCM + Mito (500 μg/rat intramyocardial injection by day-21 after DCM induction)] and euthanized by day 60. In vitro studies showed that exogenously-transferred Mito was abundantly identified in H9C2 cells. The q-PCR showed significant increase in relative number of mitDNA in Mito-transferred H9C2 cells than in control group (P
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Split-crest technique with inlay bone block grafts for narrow posterior mandibles: a retrospective clinical study with a 3-year follow-up.
In this study, 56 healthy patients underwent SCT to augment the alveolar ridge width. Implant placement was performed 3 months after SCT, and the implants were restored 3 months after placement. A planned follow-up was performed to analyze various clinical features, including X-ray radiographs, alveolar ridge width, and keratinized mucosal width, after SCT to evaluate the success of the procedure. The incisions healed well in all patients. The average initial alveolar ridge width was 2.78 ± 0.56 mm, which increased to 6.67 ± 0.60 mm after SCT. Three months later, this width declined slightly to 6.19 ± ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Development of pulmonary fibrosis after heart failure induced by elevated left atrial pressure.
CONCLUSIONS: LAP elevation resulted in chronic pulmonary edema and tissue fibrosis formation associated with pulmonary dysfunction as measured by decreased dynamic lung compliance. PMID: 32913537 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

TRIM21 causes abnormal expression of IL-6 in oral lichen planus via the TRIB2-MAPK signal axis.
Authors: Jiang C, Wei W, Wang Y, Song C, Pan L, Sun K, Du G, Deng Y, Tang G Abstract Oral lichen planus (OLP) is a common chronic inflammatory disease in the oral cavity, and has the risk of developing into oral squamous cell carcinoma (OSCC). It is necessary to discover the role of TRIM21 in the pathogenesis of OLP and its underlying mechanism. METHODS: Western bolt and qPCR assays were used to detect the effects of TRIM21 on cellular levels of ERK, p-ERK, AP-1, IL-6, TRIB2, IRF3, and IRF7, while co-immunoprecipitation was performed to verify the interaction between Trim21 and TRIB2 protein. The TRIM21 effect ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effect of rNA interference on Oatp3a1 gene expression on biological characteristics and immune factors of ovarian granulosa cells in rats with PCOS.
Authors: Yi W, Li X, Chen K, Li J, Chen K, Pan A Abstract Polycystic ovary syndrome (PCOS) is a common endocrinal metabolic disease, and its pathogenesis has not yet been thoroughly studied. The purpose of this experiment was to investigate the effect of RNA interference on Oatp3a1 gene expression on the biological viability and immune factors of ovarian granulosa cells in rats with PCOS. First, rats were intragastrically administered 1 mg/kg letrozole to successfully construct PCOS model. Western blot, qRT-PCR, CCK8 and flow cytometry were used to detect the gene expression, immune factor protein expression, cell ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Identification of three glioblastoma subtypes and a six-gene prognostic risk index based on the expression of growth factors and cytokines.
Authors: Hu B, Ruan Y, Wei F, Qin G, Mo X, Wang X, Zou D Abstract Glioblastoma multiforme (GBM) is the most common and invasive tumor of the central nervous system. Growth factors and cytokines (GFCKs) play a crucial role in tumor invasion. In the present study, GFCK expression profiles from GBM patients in the Chinese Glioma Genome Atlas were used to perform sample clustering with nonnegative matrix factorization. Three GBM subtypes were identified based on differences in GFCK expression, and the subtypes differed in characteristics and prognosis. A prognostic risk index (RI) comprising six GFCKs (BMP2, CCN3, GKN1...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research