Recent advances on the roles of epidermal growth factor receptor in psoriasis.
Authors: Wang S, Zhang Z, Peng H, Zeng K Abstract Epidermal growth factor receptor (EGFR) is a well-characterized receptor tyrosine kinase that involved in many vital activities in cell development, such as cellular homeostasis, proliferation, division, differentiation and apoptosis. Natural activation of EGFR and the concomitant downstream signaling pathways regulation are substantial to maintain normal cellular functions. In recent studies, EGFR was demonstrated to be a fundamental modulator in the control of skin inflammatory responses. Several dermatologic diseases including psoriasis are related to the anomalo...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Therapeutic targeting of immune checkpoints with small molecule inhibitors.
Authors: Smith WM, Purvis IJ, Bomstad CN, Labak CM, Velpula KK, Tsung AJ, Regan JN, Venkataraman S, Vibhakar R, Asuthkar S Abstract Immune checkpoints are known to contribute to tumor progression by enhancing cancer's ability to evade the immune system and metastasize. Immunotherapies, including monoclonal antibodies, have been developed to target specific immunosuppressive molecules on the membranes of cancer cells and have proven revolutionary in the field of oncology. Recently, small molecule inhibitors (SMIs) have gained increased attention in cancer research with potential applications in immunotherapy. SMIs h...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Biomechanical analysis of the effect of medial meniscus degenerative and traumatic lesions on the knee joint.
Authors: Li L, Yang X, Yang L, Zhang K, Shi J, Zhu L, Liang H, Wang X, Jiang Q Abstract The purpose of this study was to determine the effect of the degenerative medial meniscus and traumatic lesions on the biomechanical behavior of the knee. An elaborate three-dimensional (3D) finite element model of the total knee joint containing bones, articular cartilages, main ligaments, and menisci was developed from a combination of magnetic resonance images and computed tomography. Three types of meniscus tears were employed to represent the degenerative and traumatic lesions. The stress and meniscus extrusion of healthy a...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

CLIC1 promotes the progression of oral squamous cell carcinoma via integrins/ERK pathways.
Authors: Feng J, Xu J, Xu Y, Xiong J, Xiao T, Jiang C, Li X, Wang Q, Li J, Li Y Abstract Chloride intracellular channel 1 (CLIC1), a member of the chloride channel protein family, acts as a promoter in many malignancies, but its role in oral cancer remains unclear. Hence, this research aimed to explore the effects of CLIC1 on the progression of oral cancer cells in vitro, and we assessed its role in cell proliferation, apoptosis, migration, invasion, angiogenesis, and chemosensitivity to cisplatin and possible signaling pathways. The results demonstrated that CLIC1 depletion inhibited the proliferation, invasion, m...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Telocytes enhanced the proliferation, adhesion and motility of endometrial stromal cells as mediated by the ERK pathway in vitro.
Authors: Tang XL, Zhang FL, Jiang XJ, Yang XJ Abstract Telocytes (TCs) is special interstitial cell that have recently been identified in the female reproductive system. Endometriosis (EMs) is a benign gynecological disease whose etiology is still not fully clear. Implantation and proliferation of endometrial stromal cells (ESCs) out of the uterus are essential processes in the development of EMs. Herein, we investigate the in vitro changes of ESCs when cocultured with TCs, and the potential mechanisms involved. The current results demonstrated that, vimentin-positive/pan cytokeratin-negative ESCs, and TCs with a c...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Comparison efficacy of ESWT and Wharton's jelly mesenchymal stem cell in early osteoarthritis of rat knee.
This study compared ESWT, Wharton's jelly mesenchymal stem cells (WJMSCs) and combination of ESWT and WJMSCs therapies for early OA of the rat knee. The results showed ESWT, WJMSCs and combination of therapies significantly improved early OA knee based on analysis of pathological findings, micro-CT and immunohistochemistry (IHC) stain. The combined therapy group increased the bone volume (61.755 ± 1.537), and trabecular thickness (0.215 ± 0.014; P
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Induction of airway progenitor cells via p63 and KLF11 by Rho-kinase inhibitor Y27632 in hTERT-human nasal epithelial cells.
Authors: Kaneko Y, Konno T, Kohno T, Kakuki T, Miyata R, Ohkuni T, Kakiuchi A, Yajima R, Ohwada K, Kurose M, Himi T, Takano K, Kojima T Abstract Rho-kinase inhibitor Y27632, which is a factor in conditional reprogramming culture, induces airway progenitor clone formation. To investigate whether Y27632 enhances airway progenitor cells in nasal epithelium, primary cultures of HNECs transfected with human telomerase reverse transcriptase (hTERT-HNECs) were treated with Y27632. In TERT-HNECs treated with Y27632 for 5 days, upregulation of p63, gap junction molecules Cx26, Cx30, Cx43, cytochrome P450 enzymes CYP2C9, CYP...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Eukaryotic translation initiation factor 3 subunit G promotes human colorectal cancer.
In this study, we investigated the role of eukaryotic translation initiation factor 3 subunit G (EIF3G) in colorectal cancer. Immunohistochemical analysis showed higher EIF3G expression in stage IV human colorectal cancer tissues than in adjacent normal tissues (P
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Loss of Jak2 protects cardiac allografts from chronic rejection by attenuating Th1 response along with increased regulatory T cells.
Authors: Higazi HMKI, He L, Fang J, Sun F, Zhou Q, Huang T, He X, Wang Y, Xiong F, Yang P, Yu Q, Li J, Wagner KU, Adam BL, Zhang S, Wang CY Abstract Chronic rejection acts as the most formidable obstacle for organ transplantation in clinical settings. Herein we demonstrated in a cardiac transplantation model that blockade of Janus kinase 2 (Jak2) provides protection for cardiac allografts against chronic rejection. Specifically, loss of Jak2 almost completely abolished the production of IFN-γ+ Th1 cells, while the percentage of Foxp3+ regulatory T cells (Tregs) was significantly increased. As a result, loss o...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

The IRE1 α-XBP1 pathway function in hypoxia-induced pulmonary vascular remodeling, is upregulated by quercetin, inhibits apoptosis and partially reverses the effect of quercetin in PASMCs.
In conclusion, the study demonstrated that the IRE1α-XBP1 pathway is involved in the process of hypoxia-induced pulmonary vascular remodeling; 4u8c could restrain hypoxia-induced cell proliferation and migration and reverse the hypoxia-induced apoptosis arrest, while quercetin excited excessive ERS and the IRE1α pathway in hypoxic PASMCs and promoted apoptosis. Our data suggest that intervening the IRE1α-XBP1 pathway may be useful for hypoxia-induced pulmonary arterial hypertension therapy. PMID: 30899368 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Metformin induces the M2 macrophage polarization to accelerate the wound healing via regulating AMPK/mTOR/NLRP3 inflammasome singling pathway.
CONCLUSION: Metformin regulated AMPK/mTOR singling pathway to inhibit NLRP3 inflammasome activation, which boosted M2 macrophage polarization to accelerate the wound healing. These findings provided new insights into the molecular mechanism of metformin therapy and its therapeutic potential in wound healing. PMID: 30899369 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Protective effect of berberine on high glucose and hypoxia-induced apoptosis via the modulation of HIF-1 α in renal tubular epithelial cells.
Protective effect of berberine on high glucose and hypoxia-induced apoptosis via the modulation of HIF-1α in renal tubular epithelial cells. Am J Transl Res. 2019;11(2):669-682 Authors: Zhang X, Guan T, Yang B, Chi Z, Wan Q, Gu HF Abstract Berberine (BBR), derived from Huanglian (Coptis chinensis), is a traditional Chinese herbal medicine. In the current study, we investigated the effects of BBR in high glucose (HG) and hypoxia-induced apoptosis with normal rat renal tubular epithelial (NRK-52E) and human kidney proximal tubular cells (HK-2) and further explored the underlying molecular mechanism...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

STOML2 as a novel prognostic biomarker modulates cell proliferation, motility and chemo-sensitivity via IL6-Stat3 pathway in head and neck squamous cell carcinoma.
Authors: Qu H, Jiang W, Wang Y, Chen P Abstract STOML2 (Stomatin-like protein 2) is up-regulated and acts as an oncogenic protein in multiple cancers. However, the role and regulatory mechanism of STOML2 in head and neck squamous cell carcinoma remain unclear. Here, we found that STOML2 is overexpressed and indicates poor outcomes in HNSCC. In addition, the expression of STOML2 correlates positively with T stage, lymph node metastasis and recurrence. Reduced STOML2 dramatically inhibits cell proliferation, colony formation and motility of HNSCC cells in vitro. Furthermore, the sensitivity of HNSCC cells towards cis...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Sequential treatment of phenethyl isothiocyanate increases sensitivity of Temozolomide resistant glioblastoma cells by decreasing expression of MGMT via NF- κB pathway.
CONCLUSION: Altogether, the present research established that combination of PEITC with TMD could enhance its clinical efficacy in resistant GBM by suppressing MGMT via inhibiting NF-κB activity. PMID: 30899372 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Nitidine Chloride inhibits cell proliferation and invasion via downregulation of YAP expression in prostate cancer cells.
Authors: Shi Y, Cao T, Sun Y, Xia J, Wang P, Ma J Abstract Nitidine chloride (NC) exhibits tumor suppressive function in a variety of human cancers. However, the molecular mechanism of NC-triggered anti-cancer activity has not been fully elucidated. In the present study, we aim to investigate the anti-tumor molecular basis of NC in prostate cancer cells. Multiple approaches including MTT, FACS, wound healing assay, Transwell invasion assay, Transfection, and Western blotting were performed. We found that NC inhibited cell growth and induced apoptosis in prostate cancer cells. Moreover, NC suppressed cell migration ...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

The critical role of Dectin-1 in host controlling systemic Candida krusei infection.
Authors: Chen SM, Zou Z, Qiu XR, Hou WT, Zhang Y, Fang W, Chen YL, Wang YD, Jiang YY, Shen H, An MM Abstract There are increasing invasive fungal infections associated with non-albicans, which causes mortal infections in immune deficiency population. Candida krusei is a major non-albicans that exhibits intrinsic resistance to fluconazole and makes clinical treatment difficult. Previous studies revealed that C-type lectin receptors (CLRs) Dectin-1 plays critical roles in host defense against C. albicans infections. C. krusei and C. albicans are phylogenetically different although in the same genus. Whether Dectin-1 ...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

CCL2-CCL5/CCR4 contributed to radiation-induced epithelial-mesenchymal transition of HPAEpiC cells via the ERK signaling pathways.
Authors: Zhong Y, Lin Z, Lu J, Lin X, Xu W, Wang N, Huang S, Wang Y, Zhu Y, Chen Z, Lin S Abstract Radiation-induced lung toxicity, including radiation pneumonitis and pulmonary fibrosis, often occurs in patients receiving radiation therapy. Epithelial-mesenchymal transition (EMT) of alveolar epithelial cells (AECs) plays critical roles in radiation-induced lung toxicity. In the present study, RNA sequencing was applied to examine the whole transcriptomes of human pulmonary AEC cells (HPAEpiC) with or without radiation treatment. We found that cytokine, chemokine and cell adhesion signaling pathways were enriched i...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

HDAC I inhibitor regulates RUNX2 transactivation through canonical and non-canonical Wnt signaling in aortic valvular interstitial cells.
CONCLUSIONS: MS-275 critically regulates RUNX2 transactivation in VICs through both canonical and non-canonical Wnt signaling pathways. PMID: 30899376 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Decreased expression of miR-184 restrains the growth and invasion of endometrial carcinoma cells through CDC25A-dependent Notch signaling pathway.
Authors: Chen Z, Zhu Y, Fan X, Liu Y, Feng Q Abstract Endometrial carcinoma (EC) is one of the most common malignancies of female reproductive tract in developed countries. MicroRNA is frequently dysregulated in human cancers and acts a key regulator role in tumor cell growth and metastasis. The aims of this study were to investigate the roles of microRNA-184 (miR-184) in EC cells and to identify its potential molecular mechanism. Here, the data revealed that miR-184 was significantly downregulated in human EC tissue compared with normal endometrial tissue, and the level of miR-184 expression was associated with ly...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Gemcitabine induces Epithelial-to-Mesenchymal Transition in patient-derived pancreatic ductal adenocarcinoma xenografts.
Authors: Bulle A, Dekervel J, Libbrecht L, Nittner D, Deschuttere L, Lambrecht D, Van Cutsem E, Verslype C, van Pelt J Abstract There is a lack of well-characterized models for pancreatic ductal adenocarcinoma (PDAC). PDAC itself is unique because of its pronounced tumor microenvironment that influences tumor progression, behavior and therapeutic resistance. Here we investigated, in patient-derived tumor xenograft (PDTX) models developed from fine needle biopsies, the cancer cells behavior, Epithelial-to-Mesenchymal Transition (EMT) and drug response. For this, we studied two behaviorally distinct PDTX models. Tumo...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Exosomes from miRNA-126-modified ADSCs promotes functional recovery after stroke in rats by improving neurogenesis and suppressing microglia activation.
Authors: Geng W, Tang H, Luo S, Lv Y, Liang D, Kang X, Hong W Abstract Although adipose derived stem cells (ADSCs) exert their therapeutic potential in ischemic stroke, the migration of ADSCs in injured area is not apparently observed after intravenous administration. ADSCs are an important source of exosomes which hold great promise as an endogenous drug delivery system for the treatment of cerebral ischemia given their ability to cross the blood-brain barrier. Here we investigated whether ADSCs-derived exosomes mediated miRNAs transfer and thus promoted neurological recovery after stroke. We first proved that miR...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Long non-coding RNA FLVCR1-AS1 sponges miR-155 to promote the tumorigenesis of gastric cancer by targeting c-Myc.
CONCLUSION: FLVCR1-AS1 acted as an oncogene in GC via FLVCR1-AS1-miR-155-c-Myc signaling and may serve as a novel therapeutic target for treatment of patients with GC. PMID: 30899380 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

SXBX pill suppresses homocysteine-induced vascular smooth muscle cells dedifferentiation by inhibiting NLRP3 inflammasomes activation via ERK/p38 MAPK pathways.
In conclusion, SXBX pill inhibited Hcy-induced proliferation, migration and dedifferentiation of VSMCs by suppressing NLRP3 inflammasomes activation via of ERK/p38 MAPK pathway. PMID: 30899381 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Exploring potential biomarkers of early stage esophageal squamous cell carcinoma in pre- and post-operative serum metabolomic fingerprint spectrum using 1H-NMR method.
Authors: Yang Z, Liu Y, Ma L, Wen X, Ji H, Li K Abstract Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent types of upper gastrointestinal malignancy. Here, we used 1H nuclear magnetic resonance spectroscopy (1H-NMR) to identify potential pre- and post-operative serum biomarkers in patients with early stage ESCC using metabolomic fingerprint spectrum. Serum samples from preoperative patients with ESCC (ESCC, n = 25), postoperative patients with ESCC (PO, n = 24), and controls (n = 40) were analysed using 1H-NMR spectroscopy. Using orthogonal partial least squares-discriminant analysis, 31 alter...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Dental pulp stem cells expressing SIRT1 improve new bone formation during distraction osteogenesis.
In conclusion, SIRT1-modified DPSCs in DO was more effective to promote new bone formation during DO, which provides evidence for further investigation about the role of of SIRT1 in the DO. PMID: 30899383 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Dual-specificity phosphatase 5 suppresses ovarian cancer progression by inhibiting IL-33 signaling.
Authors: Wang L, Hu J, Qiu D, Gao H, Zhao W, Huang Y, Jiang T, Zhou J, Chen Y Abstract Ovarian cancer (OC) is the leading cause of death from gynecological malignancy. Dual-specificity phosphatases (DUSPs) are proteins that are reported involved in carcinogenesis, but their roles in OC have not be extensively studied. Here, we found that DUSP5 is markedly down-regulated in OC tissues. We reanalyzed DUSP5 expression in OC using published microarray data from the Gene Expression Omnibus (GEO) database and found that patients with low DUSP5 expression have significantly shorter overall survival than those with high expression (P
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Niclosamide ethanolamine protects kidney in adriamycin nephropathy by regulating mitochondrial redox balance.
Authors: Han P, Yuan C, Wang Y, Wang M, Weng W, Zhan H, Yu X, Wang T, Li Y, Yi W, Shao M, Li S, Yi T, Sun H Abstract Chronic kidney disease (CKD) is commonly characterized by proteinuria and leads to progressive glomerulosclerosis and tubulointerstitial fibrosis. Accumulating evidence implicates mitochondrial dysfunction including reactive oxygen species (ROS) overproduction in the pathogenesis of CKD. Mitochondrial function and ROS production are regulated by mitochondrial uncoupling. Niclosamide ethanolamine salt (NEN) is a mild mitochondrial uncoupler, which reduces urinary albumin excretion in mice with diabeti...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Herbacetin treatment remitted LPS induced inhibition of osteoblast differentiation through blocking AKT/NF- κB signaling pathway.
Herbacetin treatment remitted LPS induced inhibition of osteoblast differentiation through blocking AKT/NF-κB signaling pathway. Am J Transl Res. 2019;11(2):865-874 Authors: Cai P, Cai T, Li X, Fan L, Chen G, Yu B, Liu T Abstract Inflammation, a common situation during the process of bone healing, is reported to play a negative role in bone regeneration. Up to date, therapeutic strategies for inflammation triggered inhibition of osteoblast differentiation are still limited. The aim of this study was to explore the potential roles and molecular mechanisms of Herbacetin in the process of osteoblast...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Inhibition of ferroptosis processes ameliorates cognitive impairment in kainic acid-induced temporal lobe epilepsy in rats.
In this study, it was demonstrated that ferroptosis occurs in the hippocampus following kainic acid (KA)-induced TLE in rats. Treatment with ferrostatin-1, a specific inhibitor of ferroptosis, prevented the initiation and progression of ferroptosis in the hippocampus of KA-treated rats. This was through decreased expression of glutathione peroxidase 4, glutathione (GSH) depletion as well as lipid peroxides and iron accumulation. It was also found that ferrostatin-1 prevented hippocampal neuronal loss and rescued cognitive function in KA-induced TLE in rats. These results suggest that ferroptosis is involved in the cognitiv...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-613 induces the sensitivity of gastric cancer cells to cisplatin through targeting SOX9 expression.
Authors: Xue M, Li G, Sun P, Zhang D, Fang X, Li W Abstract Increasing evidences have suggested that deregulated miRNAs may involve in drug chemoresistance in a lot of human cancers. However, the role of miR-613 in drug chemoresistance of GC cell is still unknown. The expression of miR-613 and Sex-determining region Y (SRY)-box 9 (SOX9) in GC tissues and cell lines was detected by using qRT-PCR. Cell migration and viability were measured by the wound healing assay and CCK-8 assays. Western blot and dual-luciferase reporter were done to identify the target gene of miR-613. We showed that miR-613 expression was downr...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Long noncoding RNA LINC01296 induces non-small cell lung cancer growth and progression through sponging miR-5095.
Authors: Hu X, Duan L, Liu H, Zhang L Abstract Long noncoding RNAs (lncRNAs) played in authentic biological cell roles such as cell apoptosis, cycle, differentiation, development, migration and invasion. However, the expression pattern and function of a new lncRNA LINC01296 in non-small cell lung cancer (NSCLC) are unknown and need to be studied. In our study, we indicate that the expression of LINC01296 was overexpressed in NSCLC samples compared to adjacent non-tumor tissues. Ectopic expression of LINC01296 promoted NSCLC cell proliferation and migration. Moreover, we demonstrated that LINC01296 has a potential b...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Inhibition of KiSS-1 on metastasis of nasopharyngeal carcinoma implant tumor in nude mice.
CONCLUSION: This study may throw light on the treatment of NPC and may help improve the prognosis of patients with NPC. PMID: 30899390 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Phasic change and apoptosis regulation of JAK2/STAT3 pathway in a type 2 diabetic rat model.
We examined the interactions of metformin and sitagliptin treatment with the JAK2/STAT3 pathway and cardiac remodeling. A T2DM rat model was induced by high fat diet/streptozotocin (HFD/STZ) and treated with metformin, sitagliptin (10 mg/d or 20 mg/d) or a placebo. Cell inflammation markers, cardiac remodeling and cardiomyocyte apoptosis were evaluated. We observed an activated inflammation reaction as well as activation of the JAK2/STAT3 thought-out the experiment in the simple HFD group only in the early stage of the disease (until week 9). JAK2/STAT3 activity showed a phasic peculiarity as increased inflammation was obs...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

The anti-tumour effect of Mel and its role in autophagy in human hepatocellular carcinoma cells.
In this study, we aimed to further discuss the role of Mel in HCC and investigate the correlation of autophagy with the effect of Mel in HCC cells. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were used to detect the viability and apoptosis of HCC cells, respectively. To examine the changes of autophagy in HCC cells treated with Mel, transmission electronmicroscope (TEM) and immunofluorescence detection were adopted. Finally, we used western blot method to detect the changes of pivotal proteins in autophagy and mitochondrial apoptotic pathways. The results of MTT assay and flow cytometry revealed that Mel co...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Gut microbiota specific signatures are related to the successful rate of bariatric surgery.
Authors: Gutiérrez-Repiso C, Moreno-Indias I, de Hollanda A, Martín-Núñez GM, Vidal J, Tinahones FJ Abstract Bariatric surgery (BS) success rates vary in the long-time. A better understanding of weight-loss response may help improve the outcomes of BS. Gut microbiome could be implicated in the successful rate of BS. The aim of the study is to analyze the role of gut microbiome in the successful rate of BS. This is a cross-sectional study of a prospective cohort of 24 patients who underwent gastric bypass. Patients were classified based on excess weight loss (EWL) as: Success (EWL50% at n...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Long noncoding RNA HCP5 contributes to epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation and interacting with miR-139-5p.
Authors: Yang C, Sun J, Liu W, Yang Y, Chu Z, Yang T, Gui Y, Wang D Abstract Long noncoding RNAs (lncRNAs) play key roles in various malignancy pathogenesis. However, the mechanisms remain poorly understood in the development and progression of colorectal cancer (CRC). Here, we focused on the specific role of human leukocyte antigen (HLA) Complex P5 (HCP5) in CRC. Quantitative real-time PCR (qRT-PCR) analysis and western blot were used to assess the expression of HCP5 in CRC tissues. The association between the expressions of HCP5 and miR-139-5p was assessed by Pearson's correlation analysis. The prognosis of CRC p...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Brain natriuretic peptide mitigates TIMP2 induction and reinstates extracellular matrix catabolic activity via GSK3 β inhibition in glomerular podocytes exposed to a profibrogenic milieu.
Brain natriuretic peptide mitigates TIMP2 induction and reinstates extracellular matrix catabolic activity via GSK3β inhibition in glomerular podocytes exposed to a profibrogenic milieu. Am J Transl Res. 2019;11(2):964-973 Authors: Guo Q, Wang J, Ge Y, Malhotra DK, Dworkin LD, Wang P, Gong R Abstract Brain natriuretic peptide (BNP) has a demonstrable anti-fibrotic effect on diverse organ systems, including the kidney. To understand the molecular mechanism underlying this renoprotective effect, the efficacy of BNP was examined in an in vitro model of glomerular sclerosis by exposing glomerular podo...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9.
Authors: Chen Z, Liu Z, Shen L, Jiang H Abstract Fracture is the most common disease in the orthopedics. Long non-coding small nucleolar RNA host gene 7 (SNHG7) has been confirmed to enhance cell proliferation and decrease cell apoptosis in many cancers. However, the role of SNHG7 in skeletal fracture remains largely to be elucidated. In the current study, we observed SNHG7 was down-regulated in femoral neck fracture tissues. In addition, SNHG7 knockdown inhibited proliferation and migration, induced apoptosis, reduced activity in osteoblast cells in vitro. Bioinformatics analysis revealed SNHG7 acts as a molecular...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Inhibition of histone deacetylase 11 promotes human liver cancer cell apoptosis.
In conclusion, HDAC11 plays a critical role in the compromising the expression p53 in LCC, which can be reversed by the inhibition of HDAC11. To regulate HDAC11 may have therapeutic potential for LC treatment. PMID: 30899397 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Arsenic trioxide inhibits Skp2 expression to increase chemosensitivity to gemcitabine in pancreatic cancer cells.
Authors: Gao J, Wang G, Wu J, Zuo Y, Zhang J, Chen J Abstract The S-phase kinase associated protein 2 (Skp2), a member of the F-box protein family, regulates cell cycle progression and is highly expressed in pancreatic cancer (PC). Recently, we reported that arsenic trioxide (ATO) inhibited cell growth and invasion via downregulation of Skp2 in PC cells. Emerging evidence has revealed that Skp2 plays a crucial role in drug resistance in several kinds of cancers. Here, we determined whether ATO enhanced the sensitivity of PC cell lines to gemcitabine (GEM). We found that the combined treatment of ATO and GEM demonst...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

IL-22/IL-22R1 axis is involved in myocardial injury of a mouse cecal ligation and puncture model.
In this study, we aimed to explore if IL-22/IL-22R1 axis plays a role in the myocardial injury during sepsis. A cecal ligation and puncture (CLP) mouse model was established to explore the histopathological changes and to analyze the role of IL-22/IL-22R1 axis in myocardial injury during the process of sepsis. Histopathologically, myocardial injury was apparently observed with the progress of sepsis but it was improved at 72 h after surgery. On the contrary, the heart tissue in the sham group revealed injury at a limited degree at the first 8 h after surgery and then restored to normal. Results from immunohistochemical stu...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

RAB18 promotes proliferation and metastasis in hepatocellular carcinoma.
Authors: Gong T, Zhou B, Liu M, Chen X, Huang S, Xu Y, Luo R, Chen Z Abstract RAB18, a member of the Ras family, has been suggested to play a critical role in multiple biological process. However, its functions in the development of hepatocellular carcinoma (HCC) remain unknown. In the present study, the expression and biological role of RAB18 in HCC were investigated. Results showed that the expression level of RAB18 was significantly increased in HCC tissue specimens and HCC cell lines. Kaplan-Meier survival analysis showed that high RAB18 expression was correlated with poor overall survival compared to those wit...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

XPG Asp1104His polymorphism increases colorectal cancer risk especially in Asians.
In this study, we further investigated the role of Asp1104His (rs17655 G> C) in XPG on CRC risk. We genotyped the rs17655 G> C polymorphism in Chinese population comprising 1019 CRC cases and 1036 cancer-free controls. We also performed a meta-analysis to further assess the association. Overall, no significant association was detected between the rs17655 G> C and the risk of CRC. Stratified analysis also revealed no significant association. To further elucidate the association of the rs17655 with CRC susceptibility, we conducted a meta-analysis by including qualified publications and the current study. The meta-an...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

JQ1 effectively inhibits vasculogenic mimicry of pancreatic ductal adenocarcinoma cells via the ERK1/2-MMP-2/9 signaling pathway both in vitro and in vivo.
Authors: Zhuo M, Yuan C, Han T, Hu H, Cui J, Jiao F, Wang L Abstract Vasculogenic mimicry (VM) is an alternative type of blood and nutrition supply that is associated with more aggressive tumor biology and increased cancer-related mortality. However, the clinical implications of VM remain unclear in patients with pancreatic ductal adenocarcinoma (PDAC). The aim of this study was to investigate the clinical significance of VM in PDAC patients and to seek a novel and more efficient treatment strategy by targeting this unique process. Here, cluster of differentiation 34 (CD34)/periodic acid-Schiff (PAS) double-stainin...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Development and validation of a multi-color model using 3-dimensional printing technology for endoscopic endonasal surgical training.
In this study, we have designed a 3D printed multi-color model for training of endoscopic endonasal surgery, and obtained preliminary application results. METHODS: The 3D printed model contained facial skin, bony skeleton, internal carotid artery, turbinate, optic chiasm, and a special sellar base with appropriate colors. After it was printed, six otolaryngologists and neurosurgeons assessed the model. Twenty graduate students and residents from otolaryngology or neurosurgery, without prior experience in endoscopic endonasal surgery were recruited and consented for the training. The training results were recorded. The ...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Application of transvaginal sonographic elastography to distinguish endometrial cancer from benign masses.
In this study, we aim to evaluate the diagnostic value of transvaginal sonographic elastography to distinguish endometrial cancer from benign masses. A prospective study was conducted on patients with suspected intracavitary uterine focal lesions, abnormal uterine bleeding for routine ultrasound examination of the pelvis. A total of 217 patients were included and underwent both conventional B-mode sonography and elastography. Endometrial lesions were categorized into 3 types for qualitative analysis and the strain ratio (SR) was measured for quantitative analysis. Pathological examinations were also conducted to establish ...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Outcomes of arteriovenous fistula reconstruction in vascular access dysfunction.
CONCLUSION: Leaving patients with vascular access dysfunction to fate (no intervention) or AVF ligation is always simpler and easier. However, it should not be forgotten that paternity for vascular access are limited in these patients. We think that the primary target is to demonstrate AVF by physical examination and surgeon-performed detailed US and to make it again available for hemodialysis by reconstructing dysfunctional AVF using the most appropriate surgical strategy. LIMITATIONS: Retrospective, small sample size. PMID: 30899405 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Direction-changeable cage reduces X-ray exposure in treating isthmic lumbar spondylolisthesis: a retrospective study.
In this study, we retrospectively evaluated the X-ray exposure and clinical outcomes of the direction-changeable cage in transforaminal lumbar interbody fusion (TLIF). The patients were divided into the direction-changeable cage group (group A, n=79) and non-direction-changeable cage group (group B, n=84). Intraoperative implantation duration, cage position adjustment times, implantation fluoroscopy times, fluoroscopy exposure time of cage implantation, Oswestry Disability Index (ODI) and visual analogue scale (VAS) scores were recorded before and after operation at the last follow-up. CT scanning was performed to evaluate...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Specific knockdown of hippocampal astroglial EphB2 improves synaptic function via inhibition of D-serine secretion in APP/PS1 mice.
Authors: Qi L, Cui EH, Ji CM, Zhang XB, Wang ZA, Sun YZ, Xu JC, Zhai XF, Chen ZJ, Li J, Zheng JY, Yu RT Abstract Increasing evidence emphasizes the protective role of Eph receptors in synaptic function in the pathological development of Alzheimer's disease (AD); however, their roles in the regulation of hippocampal astrocytes remain largely unknown. Here, we directly investigated the function of astroglial EphB2 on synaptic plasticity in APP/PS1 mice. Using cell isolation and transgene technologies, we first isolated hippocampal astrocytes and evaluated the expression levels of ephrinB ligands and EphB receptors. T...
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Upregulation of long non-coding RNA SNHG6 promote esophageal squamous cell carcinoma cell malignancy and its diagnostic value.
CONCLUSION: SNHG6 may exert oncogenic function in ESCC and may be a potential diagnostic marker for this cancer. PMID: 30899408 [PubMed] (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - March 24, 2019 Category: Research Tags: Am J Transl Res Source Type: research