Query: siRNA

This page shows you your search results in order of date. This is page number 17.

Order by Relevance | Date

Total 13771 results found since Jan 2013.

Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference Engineering
Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we...
Source: Proceedings of the National Academy of Sciences - March 20, 2018 Category: Science Authors: Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Leemor Joshua-Tor, Paula T. Hammond Tags: PNAS Plus Source Type: research

GSE83634 Inhibition of NF- κB signaling by a novel siRNA sequence induces apoptosis and suppresses tumor growth in KRAS mutant colorectal cancer.
In this study, we aimed to discover an effective therapeutic microRNA (miRNA) that could target KRAS mutant CRC. For this purpose, we investigated the functional relevance of dysregulated miRNAs in KRAS mutant cancers. We transfected exogenous KRASG12V into human embryonic kidney 293 (HEK293) cells and human lung fibroblasts (MRC5) cells, and performed comprehensive microRNA expression profiling by microarray analysis. The results showed that 6 miRNAs are significantly upregulated in KRAS-transfected HEK293 and MRC5 cells. Among 6 miRNAs, we identified mature miRNA-29b-1-5p as potent growth inhibitor in CRC cell proliferat...
Source: GEO: Gene Expression Omnibus - March 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Antitumor activity of kinetochore-associated protein 2 siRNA against lung cancer patient-derived tumor xenografts.
Authors: Makita Y, Teratani M, Murata S, Hoashi Y, Matsumoto S, Kawamata Y Abstract It has been widely reported that patient-derived tumor xenografts (PDXs) are more similar to tumor tissues than conventional cancer cell lines. Kinetochore-associated protein 2 (KNTC2) is known to be upregulated specifically in tumor tissues of cancer patients and is recognized as a potential target for cancer therapy. Previously, in vivo antitumor activities of KNTC2 short interfering RNA encapsulated into a lipid nanoparticle (KNTC2-LNP) were reported in orthotopic hepatocellular carcinoma mouse models. However, it remains unclear...
Source: Oncology Letters - March 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Structural basis of siRNA recognition by TRBP double-stranded RNA binding domains
The accurate cleavage of pre-micro(mi)RNAs by Dicer and mi/siRNA guide strand selection are important steps in forming the RNA-induced silencing complex (RISC). The role of Dicer binding partner TRBP in these processes remains poorly understood. Here, we solved the solution structure of the two N-terminal dsRNA binding domains (dsRBDs) of TRBP in complex with a functionally asymmetric siRNA using NMR, EPR, and single-molecule spectroscopy. We find that siRNA recognition by the dsRBDs is not sequence-specific but rather depends on the RNA shape. The two dsRBDs can swap their binding sites, giving rise to two equally populat...
Source: EMBO Journal - March 15, 2018 Category: Molecular Biology Authors: Masliah, G., Maris, C., König, S. L., Yulikov, M., Aeschimann, F., Malinowska, A. L., Mabille, J., Weiler, J., Holla, A., Hunziker, J., Meisner-Kober, N., Schuler, B., Jeschke, G., Allain, F. H.-T. Tags: RNA Biology, Structural Biology Articles Source Type: research

DNA nanoclew templated spherical nucleic acid for siRNA delivery
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C7CC09257A, CommunicationWeimin Ruan, Meng Zheng, Yang An, Yuanyuan Liu, David Lovejoy, Mingcong Hao, Yan Zou, Albert Lee, Shu Yang, Yiqing Lu, Marco Morsch, Roger Chung, Bingyang Shi A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 14, 2018 Category: Chemistry Authors: Weimin Ruan Source Type: research

Near-infrared light-activatable siRNA delivery by microcapsules for combined tumour therapy.
Authors: Rui Y, Pang B, Zhang J, Liu Y, Hu H, Liu Z, Ama Baidoo S, Liu C, Zhao Y, Li S Abstract A polyelectrolyte microcapsule-based layer-by-layer (LbL) technique has been widely used as a multifunctional vehicle for combined tumor therapy. Meanwhile, with the rapid development of combined tumour therapy, the challenge for designing multifunctional drug delivery system has attracted much more attention. Herein, we developed a new type of microcapsule (MC) system called MPA@siRNA@DOX@MC, which conjugated with siRNA and DOX as well as ICG-Der-02 (MPA) by electrostatic absorption. MPA as indocyanine green (ICG) fluor...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 14, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

"siRNA traffic lights": Arabino-configured 2'-anchors for fluorescent dyes are key for dual color readout in cell imaging
Org. Biomol. Chem., 2018, Accepted Manuscript DOI: 10.1039/C8OB00417J, PaperJeannine Steinmeyer Steinmeyer, Heidi-Kristin Walter, Mathilde Bichelberger, Violetta Schneider, Tomas Kubar, Franziska Ronicke, Bettina Olshausen, Karin Nienhaus, Gerd Ulrich Nienhaus, Ute Schepers, Marcus Elstner, Hans-Achim Wagenknecht Two fluorescent dyes are covalently attached in diagonal interstrand orientation to siRNA undergo an energy transfer and thereby enable a dual color fluorescent readout (red/green) for hybridization. Three different structural... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - March 13, 2018 Category: Molecular Biology Authors: Jeannine Steinmeyer Steinmeyer Source Type: research

A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy
Chem. Commun., 2018, Advance Article DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shu-Meng Bai, Chun-Hua Lu, Huang-Hao Yang A new siRNA delivery platform based on black phosphorus nanosheets is constructed which shows excellent therapy efficiency for tumors. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 12, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research

A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy.
Abstract Gene therapy with small interfering RNA (siRNA) has been proved to be a promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery system that has high efficiency in transporting siRNA without obvious side effects remains a challenge. Herein, we designed a new survivin siRNA delivery system based on polyethyleneimine functionalized black phosphorus (BP) nanosheets which could suppress tumor growth by silencing survivin expression. Combined with the photothermal properties of the BP nanosheets, the presented delivery system shows excellent ...
Source: Chemical Communications - March 12, 2018 Category: Chemistry Authors: Wang H, Zhong L, Liu Y, Xu X, Xing C, Wang M, Bai SM, Lu CH, Yang HH Tags: Chem Commun (Camb) Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specifi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - March 6, 2018 Category: Biochemistry Source Type: research

101PDelivery of E-cadherin (CDH1) gene and epidermal growth factor receptor (EGFR) siRNA, using carbonate apatite as a promising vehicle in treatment of breast cancer
Background: E-cadherin (CDH1), a cell adhesion glycoprotein is widely associated with breast cancer where it is involved in tumor growth, invasion and metastasis. Down regulation of its gene is hall mark for epithelial-mesenchymal transition, which is an essential process for tumor metastasis. While various pathways are associated with E-cadherin, one of the prominent signaling pathways is via epidermal growth factor reception (EGFR), which is found to be over expressed. Silencing the over expressed EGFR gene by using a specific short-interfering RNA (siRNA), or inducing the expression of CDH1 gene with the help of a plasm...
Source: Annals of Oncology - March 5, 2018 Category: Cancer & Oncology Source Type: research

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.
Authors: Capel V, Vllasaliu D, Watts P, Clarke PA, Luxton D, Grabowska AM, Mantovani G, Stolnik S Abstract Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform for pulmonary delivery. The system is based on piperazine-substituted chitosans, a material designed to integrate established, safe application of chitosan for mucosal administration with novel properties: the piperazine-substituted chitosans are freely water-solub...
Source: Drug Delivery - March 3, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

Black Phosphorus Nanosheets-Based siRNA Delivery System for Synergistic Photothermal and Gene Therapy
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shumeng Bai, Chun-Hua Lu, Huanghao Yang Gene therapy with small interfering RNA (siRNA) has been proved to be the promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 2, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research

siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells
Publication date: May 2018 Source:Micron, Volume 108 Author(s): Janine Berkholz, Regina Eberle, Klaus Boller, Barbara Munz skNAC (skeletal and heart muscle-specific variant of nascent polypeptide-associated complex) and Smyd1 (SET and MYND domain-containing 1) form a protein dimer which is specific for striated muscle cells. Its function is largely unknown. On the one hand, skNAC-Smyd1 appears to control transcriptional processes in the nucleus, on the other hand, specifically at later stages of myogenic differentiation, both proteins translocate to the sarcoplasm and at least Smyd1 specifically associates with sarcomeric...
Source: Micron - March 1, 2018 Category: Biology Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research

Preparation of PEGylated cationic nanoliposome-siRNA complexes for cancer therapy.
In conclusion, our novel PEGylated liposomes have high potential for systemic delivery of siRNA and can improve in vivo stability of free siRNA and also siRNA lipoplexes. PMID: 29475393 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - February 26, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A dual targeting drug delivery system for penetrating blood brain barrier and selectively delivering siRNA to neurons for Alzheimer's disease treatment.
CONCLUSION: In conclusion, this study preliminarily demonstrated the ApoA-I and NL4 dual modified dendrimer nanoparticles were efficient carrier for siRNA delivery to AD bearing brain. PMID: 29484985 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - February 26, 2018 Category: Biotechnology Authors: Zhang C, Gu Z, Shen L, Liu X, Lin H Tags: Curr Pharm Biotechnol Source Type: research

i-Fect Delivers Again and Again
This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.A mixture of ouri-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita.Extracellular Lactate Dehydrogenase A Release ...
Source: Neuromics - February 19, 2018 Category: Neuroscience Tags: angiogenesis Delivering siRNA to the CNS Lactate Hydrogenase A LDHA Neurodegenerative Disease siRNA delivery in-vivo Source Type: news

Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research

Exosomes serve as nanoparticles to suppress tumor growth and angiogenesis in gastric cancer by delivering hepatocyte growth factor siRNA
Exosomes derived from cells have been found to mediate signal transduction between cells and to act as efficient carriers to deliver drugs and small RNA. Hepatocyte growth factor (HGF) is known to promote the growth of both cancer cells and vascular cells, and the HGF‐cMET pathway is a potential clinical target. Here, we characterized the inhibitory effect of HGF siRNA on tumor growth and angiogenesis in gastric cancer. In addition, we showed that HGF siRNA packed in exosomes can be transported into cancer cells, where it dramatically downregulates HGF expression. A cell co‐culture model was used to show that exosomes ...
Source: Cancer Science - February 11, 2018 Category: Cancer & Oncology Authors: Haiyang Zhang, Yi Wang, Ming Bai, Junyi Wang, Kegan Zhu, Rui Liu, Shaohua Ge, JiaLu Li, Tao Ning, Ting Deng, Qian Fan, Hongli Li, Wu Sun, Guoguang Ying, Yi Ba Tags: ORIGINAL ARTICLE Source Type: research

Effects of Grb2-associated binding protein 2-specific siRNA on the migration and invasion of MG-63 osteosarcoma cells.
Authors: Wang H, He H, Meng H, Cui Y, Wang W Abstract To investigate the association between the expression of growth factor receptor binding protein 2-associated binding protein 2 (Gab2) in human osteosarcoma as well as the effects of Gab2 on invasion and metastasis, human MG-63 osteosarcoma cells were transfected with small interfering (si)RNA plasmid. Gab2 protein and mRNA expression levels were detected using western blotting and reverse transcription-polymerase chain reaction, respectively. The cell migration and invasion abilities were detected using in vitro chemotaxis and invasion assays, respectively, foll...
Source: Oncology Letters - February 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Engineering functional inorganic-organic hybrid systems: advances in siRNA therapeutics
Chem. Soc. Rev., 2018, Advance Article DOI: 10.1039/C7CS00479F, Review ArticleJianliang Shen, Wei Zhang, Ruogu Qi, Zong-Wan Mao, Haifa Shen Engineering functional inorganic-organic hybrid vectors for siRNA delivery systems as a promising approach to overcome many physiological and biological obstacles, which greatly paves the way for a successful, safe and efficient platform in clinical applications. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chemical Society Reviews - February 8, 2018 Category: Chemistry Authors: Jianliang Shen Source Type: research

Genes, Vol. 9, Pages 74: Covalent Strategies for Targeting Messenger and Non-Coding RNAs: An Updated Review on siRNA, miRNA and antimiR Conjugates
Eritja Oligonucleotide-based therapy has become an alternative to classical approaches in the search of novel therapeutics involving gene-related diseases. Several mechanisms have been described in which demonstrate the pivotal role of oligonucleotide for modulating gene expression. Antisense oligonucleotides (ASOs) and more recently siRNAs and miRNAs have made important contributions either in reducing aberrant protein levels by sequence-specific targeting messenger RNAs (mRNAs) or restoring the anomalous levels of non-coding RNAs (ncRNAs) that are involved in a good number of diseases including cancer. In addition t...
Source: Genes - February 6, 2018 Category: Genetics & Stem Cells Authors: Santiago Grijalvo Adele Alagia Andreia Jorge Ramon Eritja Tags: Review Source Type: research

Nanotechnology-Based Strategies for siRNA Brain Delivery for Disease Therapy.
Abstract Small interfering RNA (siRNA)-based gene silencing technology has demonstrated significant potential for treating brain-associated diseases. However, effective and safe systemic delivery of siRNA into the brain remains challenging because of biological barriers such as enzymatic degradation, short circulation lifetime, the blood-brain barrier (BBB), insufficient tissue penetration, cell endocytosis, and cytosolic transport. Nanotechnology offers intriguing potential for addressing these challenges in siRNA brain delivery in conjunction with chemical and biological modification strategies. In this review, ...
Source: Trends in Biotechnology - February 5, 2018 Category: Biotechnology Authors: Zheng M, Tao W, Zou Y, Farokhzad OC, Shi B Tags: Trends Biotechnol Source Type: research

PARP1-siRNA suppresses human prostate cancer cell growth and progression.
Authors: Lai Y, Kong Z, Zeng T, Xu S, Duan X, Li S, Cai C, Zhao Z, Wu W Abstract Poly (ADP-ribose) polymerase (PARP) inhibitors, such as olaparib or rucaparib, have shown treatment efficacy in BRCA1/2-deficient tumors. However, since PARP inhibitors (PARPi) mainly modulate the activation of PARP but not its expression, whether small interfering RNA (siRNA) specific to PARP has the same function as PARPi has not been well defined. In the present study it was demonstrated that PARP1-siRNA could reduce prostate cancer (PCa) cell progression regardless of the BRCA1/2 mutation. PARP1 silencing could significantly inhibi...
Source: Oncology Reports - February 4, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Noncovalent tagging of siRNA with steroids for transmembrane delivery.
Abstract Short interfering RNA (siRNA) has broad applications in biology and medicine, and holds tremendous potential to become a new class of therapeutics for many diseases. As a highly anionic macrobiomolecule, its cytosolic delivery, however, has been a major roadblock in translation. Here, we report the development of small, bifunctional chemical tags capable of transporting siRNA directly into the cytosol. The bifunctional tags consist of a siRNA-binding moiety that interacts with siRNA non-covalently, and a steroid domain that readily fuses with the mammalian cell membrane. In contrast to the conventional co...
Source: Biomaterials - February 3, 2018 Category: Materials Science Authors: Tai W, Gao X Tags: Biomaterials Source Type: research

Dual-responsive polyplexes with enhanced disassembly and endosomal escape for efficient delivery of siRNA.
Abstract Despite the extracellular barriers for siRNA delivery have been overcome by utilizing advanced nanoparticle delivery systems, the key intracellular barriers after internalization including efficient disassembly of siRNA and endosomal escape still remains challenging. To address the issues, we developed a unique pH- and redox potential-responsive polyplex delivery system based on the copolymer of mPEG-b-PLA-PHis-ssPEI1.8 k, which is composed of a pH-responsive copolymer of PEG-b-PLA-PHis (Mw 5 k) and a branched PEI (Mw1.8 k) linked with redox cleavable disulfide bond. The copolymer showed excellent s...
Source: Biomaterials - February 3, 2018 Category: Materials Science Authors: Zhu J, Qiao M, Wang Q, Ye Y, Ba S, Ma J, Hu H, Zhao X, Chen D Tags: Biomaterials Source Type: research

Recent advances in siRNA delivery for cancer therapy using smart nanocarriers.
Abstract Small interfering RNAs (siRNAs) can selectively target and downregulate disease-causing genes, holding great promise in treating human diseases, especially malignant cancers. However, how to efficiently deliver siRNAs into target cell cytosol is a problem that has hindered their clinical application. Here, we review the recent strategies for siRNA delivery on the basis of smart nanocarriers by using stimuli-responsive materials. We highlight the rationales of how to design smart nanocarriers responsive to physiological and external stimuli to improve the delivery efficiency, targeting precision and gene s...
Source: Drug Discovery Today - January 23, 2018 Category: Drugs & Pharmacology Authors: Zhang P, An K, Duan X, Xu H, Li F, Xu F Tags: Drug Discov Today Source Type: research

GSE93608 Comparison of HBO1 siRNA-treated Human Umbilical Vein Endothelial Cells (HUVECs) and control siRNA-treated HUVECs
Contributors : Philip A Marsden ; Matthew S YanSeries Type : Expression profiling by array ; Non-coding RNA profiling by arrayOrganism : Homo sapiensTo determine the role of HBO1 in EC physiology, gene expression analysis was conducted on control and HBO1 siRNA-treated HUVECs. A total of 263 differentially regulated protein-coding transcripts were detected, many of which are key for growth and angiogenesis. Additionally, many genes involved in cell cycle, cell division, and DNA replication were dysregulated. HBO1-regulated genes were verified by qRT-PCR, including those with roles in vessel tone regulation (e.g. ACE1), ves...
Source: GEO: Gene Expression Omnibus - January 19, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Non-coding RNA profiling by array Homo sapiens Source Type: research

Cyclam-Modified PEI for Combined VEGF siRNA Silencing and CXCR4 Inhibition To Treat Metastatic Breast Cancer.
Abstract Chemokine receptor CXCR4 plays an important role in cancer cell invasion and metastasis. Recent findings suggest that anti-VEGF therapies upregulate CXCR4 expression, which contributes to resistance to antiangiogenic therapies. Here, we report the development of novel derivatives of polyethylenimine (PEI) that effectively inhibit CXCR4 while delivering anti-VEGF siRNA. PEI was alkylated with different amounts of a CXCR4-binding cyclam derivative to prepare PEI-C. Modification with the cyclam derivatives resulted in a considerable decrease in cytotoxicity when compared with unmodified PEI. All the PEI-C sh...
Source: Biomacromolecules - January 19, 2018 Category: Biochemistry Authors: Zhou Y, Yu F, Zhang F, Chen G, Wang K, Sun M, Li J, Oupický D Tags: Biomacromolecules Source Type: research

Potential Molecular Targets in the Treatment of Lung Cancer Using siRNA Technology.
This article dedicated to an update review of molecular targets could potentially be used for target therapy of lung cancer using SiRNA technology. PMID: 29336624 [PubMed - as supplied by publisher]
Source: Cancer Investigation - January 18, 2018 Category: Cancer & Oncology Tags: Cancer Invest Source Type: research

Highly Stable RNA Capture by Dense Cationic Polymer Brushes for the Design of Cytocompatible, Serum-Stable SiRNA Delivery Vectors.
Abstract The high density of polymer brushes confers to these coatings unique physico-chemical properties, in particular for the regulation of biomolecular interaction and the design of highly selective coatings for biosensors and protein patterning. Here we show that high density poly(dimethylaminoethyl methacrylate) cationic polymer brushes enable the stable uptake of high levels of oligonucleotides. This is proposed to result from the high degree of crowding and associated increase in entropic driving force for the binding of polyelectrolytes such as nucleic acid molecules. We further demonstrate the ease with ...
Source: Biomacromolecules - January 16, 2018 Category: Biochemistry Authors: Li D, Sharili AS, Connelly J, Gautrot JE Tags: Biomacromolecules Source Type: research

Ruthenium dendrimers as carriers for anticancer siRNA.
This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Michlewska S, Ionov M, Maroto-Díaz M, Szwed A, Ihnatsyeu-Kachan A, Loznikova S, Shcharbin D, Maly M, Ramirez RG, de la Mata FJ, Bryszewska M Tags: J Inorg Biochem Source Type: research

The proteoglycan biglycan mediates inflammatory response by activating TLR-4 in human chondrocytes: Inhibition by specific siRNA and high polymerized Hyaluronan.
Abstract Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. It has been reported that released biglycan is an endogenous ligand for TLR-2/4 in some cell type. We studied the role of biglycan in an experimental model of biglycan-induced inf...
Source: Archives of Biochemistry and Biophysics - January 12, 2018 Category: Biochemistry Authors: Avenoso A, D'Ascola A, Scuruchi M, Mandraffino G, Calatroni A, Saitta A, Campo S, Campo GM Tags: Arch Biochem Biophys Source Type: research

Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer.
In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFo...
Source: Drug Delivery - January 11, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

VEGF siRNA delivery by a cancer-specific cell-penetrating peptide.
In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stability and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated intracellular VEG...
Source: Journal of Microbiology and Biotechnology - January 11, 2018 Category: Biotechnology Authors: Lee YW, Hwang YE, Lee JY, Sohn JH, Sung BH, Kim SC Tags: J Microbiol Biotechnol Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - January 10, 2018 Category: Lipidology Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells.
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta - January 8, 2018 Category: Biochemistry Authors: Ohlig T, Le DV, Gardemann A, Wolke C, Gürtler S, Peter D, Schild L, Lendeckel U Tags: Biochim Biophys Acta Source Type: research

siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice.
Abstract Previous studies have shown that inflammatory mediators involved in the development of rheumatoid arthritis (RA) are regulated by the Rel/nuclear factor-κB (Rel/NF-κB) transcription factor family. c-Rel, a member of the Rel/NF-κB family that is preferentially expressed by immune cells, is a risk factor for several inflammatory diseases including RA. In the current study, we investigated whether targeting c-Rel can be used to treat collagen-induced arthritis, an animal model for RA. c-Rel specific siRNA (siRel) delivered by nanoparticles was used to knockdown the expression of c-Rel. Our results showed ...
Source: International Immunopharmacology - January 8, 2018 Category: Allergy & Immunology Authors: Fan T, Zhong F, Liu R, Chen YH, Wang T, Ruan Q Tags: Int Immunopharmacol Source Type: research

GSE100813 Transcriptome analysis of FKBP25 siRNA-knockdown in cultured HEK 293 cells
Contributors : David Dilworth ; Christopher J NelsonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPeptidyl-prolyl isomerases (PPIs) are a superfamily of ubiquitous enzymes that catalyze the cis-trans interconversion of Xaa-Pro peptide bonds. The functions of most PPIs remain uncharacterized. FKBP25, a mammalian PPI, localizes to the nucleus, binds nucleic acids, and associates with chromatin modifying enzymes. However, the role of this protein in transcriptional regulation remains unclear. To help address this question we present RNA-seq gene expression profiling in HEK293 cells tra...
Source: GEO: Gene Expression Omnibus - January 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

4 co-delivery of tnf- α sirna and il-22 via a nanoparticle-in-hydrogel system exerts synergistic therapeutic effects against ulcerative colitis
Combination therapy is an emerging approach for treating ulcerative colitis (UC). During the development of UC, TNF- α acts as the major pro-inflammatory cytokine while interleukin-22 (IL-22) plays an important role in preventing mucosal damage. In the present study, we loaded TNF-α siRNA (siTNF) into galactosylated polymeric nanoparticles (NPs). The resultant Gal-siTNF-NPs had a desirable diameter (~261 nm), narrow size distribution and a slightly negative zeta potential (~‒12 mV). These NPs successful mediated the targeted delivery of siTNF to macrophages and efficiently inhibited the expression of TNF-α.
Source: Gastroenterology - January 1, 2018 Category: Gastroenterology Authors: Bo Xiao, Emilie Viennois, Tim L. Denning, Didier Merlin Tags: Clinical and Research Challenges Source Type: research

Exosomes Serve as Nano ‐particles to Suppress Tumor Growth and Angiogenesis in Gastric Cancer by Delivering HGF siRNA
This article is protected by copyright. All rights reserved.
Source: Cancer Science - December 29, 2017 Category: Cancer & Oncology Authors: Haiyang Zhang, Yi Wang, Ming Bai, Junyi Wang, Kegan Zhu, Rui Liu, Shaohua Ge, JiaLu Li, Tao Ning, Ting Deng, Qian Fan, Hongli Li, Wu Sun, Guoguang Ying, Yi Ba Tags: Original Article Source Type: research

Improved method for synthesis of low molecular weight protamine –siRNA conjugate
Publication date: Available online 19 December 2017 Source:Acta Pharmaceutica Sinica B Author(s): Zhili Yu, Junxiao Ye, Xing Pei, Lu Sun, Ergang Liu, Jianxin Wang, Yongzhuo Huang, Seung Jin Lee, Huining He RNAi technology has aroused wide public interest due to its high efficiency and specificity to treat multiple types of diseases. However, the effective delivery of siRNA remains a challenge due to its large molecular weight and strong anionic charge. Considering their remarkable functions in vivo and features that are often desired in drug delivery carriers, biomimetic systems for siRNA delivery become an effective and ...
Source: Acta Pharmaceutica Sinica B - December 20, 2017 Category: Cancer & Oncology Source Type: research

Effects of LncRNA BC168687 siRNA on Diabetic Neuropathic Pain Mediated by P2X7 Receptor on SGCs in DRG of Rats.
In conclusion, lncRNA BC168687 may participate in the pathogenesis of DNP mediated by P2X7 receptor, which will provide a novel way for the study of the pathogenesis of diabetes mellitus complicated with neuropathic pain and its prevention and treatment. PMID: 29204447 [PubMed - in process]
Source: Biomed Res - December 7, 2017 Category: Research Authors: Liu C, Tao J, Wu H, Yang Y, Chen Q, Deng Z, Liu J, Xu C Tags: Biomed Res Int Source Type: research

siRNA delivery with chitosan: influence of chitosan molecular weight, degree of deacetylation and amine to phosphate ratio on in vitro silencing efficiency, hemocompatibility, biodistribution and in vivo efficacy.
Abstract Chitosan (CS) shows in vitro and in vivo efficacy for siRNA delivery, but with contradictory findings for incompletely characterized systems. To understand which parameters produce effective delivery, a library of precisely characterized chitosans was produced at different DDAs and Mn. Encapsulation and transfection efficiencies were characterized in vitro. Formulations were selected to examine the influence of Mn and N:P ratio on nanoparticle uptake, metabolic activity, genotoxicity, and in vitro transfection. Hemocompatibility and in vivo biodistribution were then investigated for different Mn, N:P rati...
Source: Biomacromolecules - December 6, 2017 Category: Biochemistry Authors: Alameh MG, Lavertu M, Tran-Khanh N, Chang CY, Lesage F, Bail M, Darras V, Chevrier A, Buschmann MD Tags: Biomacromolecules Source Type: research

07 The approach to generate oncolytic adenovirus by sirna mediated e1b silencing
ConclusionIn addition to knockout of the E1B coding cassette, siRNA mediated E1B also serves as an approach to prepare oncolytic adenovirus.AcknowledgementsSupported by grants from UICC (ICRETT Fellowship), Guangdong Commission of Health and Family Plan (Grant No. 2014A276).
Source: Journal of Investigative Medicine - December 5, 2017 Category: Research Authors: Zhang, X., Matskova, L., Zheng, B., Ernberg, I., He, Z., Huang, Z. Tags: Abstracts Source Type: research

Inhibition of HBV replication by delivering the dual-gene expression vector pHsa-miR16-siRNA in HepG2.2.15 cells
This study aimed to construct the dual-gene expression vector pHsa-miR16-siRNA which can express human miR-16 and HBV X siRNA, and examine its regulatory effect on HBV gene expression in the HepG2.2.15 cell line. The expression vectors siR-1583 and pHsa-miR16-siRNA were designed and constructed. HepG2.2.15 cells were transfected with the empty vector, siR-1583, pmiR-16 and pHsa-miR16-siRNA, respectively. ELISA was performed to measure the expression of HBsAg and HBeAg in the culture supernatant 48 and72 h post transfection. Fluorescence quantitative PCR was used to measure the HBV mRNA degradation efficiency and HBV DNA co...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2017 Category: Research Source Type: research

Well-defined cationic N-3-(dimethylamino)propylmethacrylamide hydrochloride based (co)polymers for siRNA delivery.
Abstract Cationic glycopolymers have shown to be excellent candidates for the fabrication of gene delivery devices due to their ability to electrostatically interact with negatively charged nucleic acids and the carbohydrate residues ensure enhanced stability and low toxicity of the polyplexes. The ability to engineer the polymers for optimized compositions, molecular weights and architectures is critical in the design of effective gene delivery vehicles. Therefore, in this study, the aqueous reversible addition-fragmentation chain transfer polymerization (RAFT) was used to synthesize well-defined cationic glycopo...
Source: Biomacromolecules - December 1, 2017 Category: Biochemistry Authors: Singhsa P, Diaz-Dussan D, Manuspiya H, Narain R Tags: Biomacromolecules Source Type: research

RNAi mechanisms in Huntington ’s disease therapy: siRNA versus shRNA
AbstractHuntington ’s Disease (HD) is a genetically dominant trinucleotide repeat disorder resulting from CAG repeats within the Huntingtin (HTT) gene exceeding a normal range (>  36 CAGs). Symptoms of the disease manifest in middle age and include chorea, dystonia, and cognitive decline. Typical latency from diagnosis to death is 20 years. There are currently no disease-modifying therapies available to HD patients. RNAi is a potentially curative therapy for HD. A popular line of research employs siRNA or antisense oligonucleotides (ASO) to knock down mutant Huntingtin mRNA (mHTT). Unfortunately, this modality requ...
Source: Translational Neurodegeneration - November 27, 2017 Category: Neurology Source Type: research

Cyclodextrin-siRNA conjugates as versatile gene silencing agents.
Abstract Functional siRNAs (luciferase and PLK1) have been conjugated to β-cyclodextrin and the ability of the conjugates to retain gene knockdown activity has been assessed by delivery to cancer cell lines using various formulations. Initially two formulations used complexation with polycations, namely Lipofectamine 2000 and an amphiphilic polycationic cyclodextrin. Gene knockdown results for human glioblastoma cells (U87) and prostate cancer cells (PC3, DU145) showed that conjugation to the cyclodextrin did not reduce gene silencing by the RNA. A third mode of delivery involved formation of targeted nanoparticl...
Source: European Journal of Pharmaceutical Sciences - November 27, 2017 Category: Drugs & Pharmacology Authors: Malhotra M, Gooding M, Evans JC, O'Driscoll D, Darcy R, O'Driscoll C Tags: Eur J Pharm Sci Source Type: research