Beta hydroxybutyrate induces lung cancer cell death, mitochondrial impairment and oxidative stress in a long term glucose-restricted condition
CONCLUSION: The induced cell death, oxidative stress and mitochondria impairment beside attenuated levels of cancer stem cell markers following BHB administration emphasize on the distinctive role of metabolic plasticity of cancer cells and propose possible therapeutic approaches to control cancer cell growth through metabolic fuels.PMID:38656394 | DOI:10.1007/s11033-024-09501-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Farzad Izak Shirian Milad Karimi Maryam Alipour Siamak Salami Mitra Nourbakhsh Samira Nekufar Nahid Safari-Alighiarloo Masoumeh Tavakoli-Yaraki Source Type: research
Root Hair Imaging Using Confocal Microscopy
Methods Mol Biol. 2024;2787:81-94. doi: 10.1007/978-1-0716-3778-4_5.ABSTRACTPlant genetics plays a key role in determining root hair initiation and development. A complex network of genetic interactions therefore closely monitors and influences root hair phenotype and morphology. The significance of these genes can be studied by employing, for instance, loss-of-function mutants, overexpression plant lines, and fluorescently labeled constructs. Confocal laser scanning microscopy is a great tool to visually observe and document these morphological features. This chapter elaborates the techniques involved in handling of micro...
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Krishna Vasant Mutanwad Monika Debreczeny Doris Lucyshyn Source Type: research
Sample Preparation and Phosphopeptide Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry
Methods Mol Biol. 2024;2787:293-303. doi: 10.1007/978-1-0716-3778-4_20.ABSTRACTPhosphopeptide enrichment is the main bottleneck of every phosphorylation study. Therefore, in this chapter, a general workflow tries to overbridge the hurdles of plant sample handling from sample collection to protein extraction, protein solubilization, enzymatic digestion, and enrichment step prior to mass spectrometry. The workflow provides information to perform global proteomics as well as phosphoproteomics enabling the researcher to use the protocol in both fields.PMID:38656498 | DOI:10.1007/978-1-0716-3778-4_20 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Gorji Marzban Eldi Sulaj Source Type: research
Atomic Force Microscopy in the Characterization of the Structure of Cell Wall Components
Methods Mol Biol. 2024;2788:81-95. doi: 10.1007/978-1-0716-3782-1_6.ABSTRACTAtomic force microscopy (AFM) has broken boundaries in the characterization of the supramolecular architecture of cell wall assemblies and single cell wall polysaccharides at the nanoscale level. Moreover, AFM provides an opportunity to evaluate the mechanical properties of cell wall material which is not possible with any other method. However, in the case of plant tissue, the critical step is a smart sample preparation that should not affect the polysaccharide structure or assembly and on the other hand should consider device limitations, especia...
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Justyna Cybulska Source Type: research
Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis
This study uses cell suspensions as a platform for expressing the cyt1Aa gene in the coffee genome (C. arabica L. var. Catuaí) to control CBB. The Agrobacterium tumefaciens strain GV3101::pMP90 containing the bar and cyt1Aa genes are used to genetically transform embryogenic cell suspensions. PCR amplification of the cyt1Aa gene is observed 2, 5, and 7 weeks after infection. This chapter describes a protocol that can be used for the development of resistant varieties against biotic and abiotic stresses and CRISPR/Cas9-mediated genome editing.PMID:38656516 | DOI:10.1007/978-1-0716-3782-1_12 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Andr és Gatica-Arias Jimmy Villalta-Villalobos Luiz Filipe Pereira Source Type: research
In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL)
Methods Mol Biol. 2024;2788:243-255. doi: 10.1007/978-1-0716-3782-1_14.ABSTRACTGamma radiation (60Co)-induced mutagenesis offers an alternative to develop rice lines by accelerating the spontaneous mutation process and increasing the pool of allelic variants available for breeding. Ionizing radiation works by direct or indirect damage to DNA and subsequent mutations. The technique can take advantage of in vitro protocols to optimize resources and accelerate the development of traits. This is achieved by exposing mutants to a selection agent of interest in controlled conditions and evaluating large numbers of plants in redu...
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Jason P érez Alejandro Hern ández-Soto Ana Abdelnour-Esquivel Walter Vargas-Segura William Watson-Guido Andr és Gatica-Arias Source Type: research
Dual-Luciferase Reporter Assay for Prescreening CRISPR (d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Human Cells
In this study, we evaluate the application of EpiEdit to plant promoters, specifically the MLO (mildew locus o) gene promoter. We use a modified CRISPR-(d)Cas9 system, in which the nuclease-deficient Cas9 (dCas9) is fused to an epigenetic modifier, to experimentally demonstrate the utility of this tool for optimizing epigenetic engineering of a plant promoter prior to in vivo plant epigenome editing. Guide RNAs are used to deliver the dCas9-epigenetic modifier fusion protein to the target gene sequence, where it induces modification of MLO gene expression. We perform preliminary experiments using a plant promoter cloned in...
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Ann-Kathrin Hinrichs Aline Koch Antje M Richter Source Type: research
Precision Genome Editing with CRISPR-Cas9
Methods Mol Biol. 2024;2788:355-372. doi: 10.1007/978-1-0716-3782-1_21.ABSTRACTThe CRISPR/Cas9 system is a revolutionary technology for genome editing that allows for precise and efficient modifications of DNA sequences. The system is composed of two main components, the Cas9 enzyme and a guide RNA (gRNA). The gRNA is designed to specifically target a desired DNA sequence, while the Cas9 enzyme acts as molecular scissors to cut the DNA at that specific location. The cell then repairs the digested DNA, either through nonhomologous end joining (NHEJ) or homology-directed repair (HDR), resulting in either indels or precise mo...
Source: Mol Biol Cell - April 24, 2024 Category: Molecular Biology Authors: Shahroz Rahman Abdul Rehman Ikram Farrukh Azeem Muhammad Tahir Ul Qamar Tayyaba Shaheen None Mehboob-Ur-Rahman Source Type: research
MiR-34a ameliorates arterial blood flow in rats with lower limb arteriosclerosis obliterans via Sirt1 signaling pathway
In this study, we investigated the impact of microRNA-34a (miR-34a) on lower limb arteriosclerosis obliterans in rats through the Sirtuin 1 (Sirt1) signaling pathway. Thirty-six Sprague-Dawley rats were divided into normal, model, and miR-34a mimics groups. Rats in the normal group were raised normally, while the model group underwent lower limb arteriosclerosis obliterans induction and received saline injections. The miR-34a mimics group also underwent arteriosclerosis obliterans modeling but received miR-34a mimics injections. Immunohistochemistry revealed significantly increased vascular endothelial growth factor (VEGF)...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Tuo Xu Changwei Zheng Yongkang Wu Zhengde Chen Huilai Miao Source Type: research
Importin 7 enhances protective autophagy induced by nuclear translocation of homeobox A10 in oral squamous cell carcinoma
This study aimed to explore the function and mechanism of IPO7 in OSCC. IPO7 expression in tissues and cells was determined by RT-qPCR. Cell proliferative, migratory, and invasive capabilities were detected through transwell assay and colony formation assay. Mice xenograft models were established for evaluating tumor growth. Autophagy was estimated by the LC3 levels in cells through western blot and immunofluorescence (IF). Western blot was utilized to detect the key proteins in PERK/EIF2AK3/ATF4 pathway for assessing the endoplasmic reticulum stress (ERS). The interaction of IPO7 and homeobox A10 (HOXA10) was tested by GS...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Shanshan Chen Jianbo Pan Source Type: research
The therapeutic effects of ADSCs on photoaging of skin induced by UVB irradiation
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):233-240. doi: 10.14715/cmb/2024.70.3.35.ABSTRACTSkin photoaging affects appearance and is associated with a variety of skin diseases, even skin cancer. Therefore, the prevention and treatment of skin photoaging is very important. However, there is a lack of effective evaluation methods, so it is an urgent problem to explore a comprehensive, non-invasive and in vivo evaluation method. Adipose-derived mesenchymal stem cells (ADSCs) are widely used to improve skin conditions as easier to obtain and positive effects. Recently, as the development of ultrasound technology, skin u...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Jing Wang Xiaochen Zhou Di Cao Ying Meng Yingchun Wang Debin Yang Source Type: research
MiR-4454 in Combined Allergic Rhinitis and Asthma Syndrome (CARAS): Clinical significance and mechanistic insights into airway inflammation
This study found that inhibiting the expression of miR-4454 can improve HDM-induced cell injury, which may be related to miR-4454 regulating the activation of IL-17/NF-кB inflammatory axis.PMID:38650129 | DOI:10.14715/cmb/2024.70.3.34 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Zhiguang Liu Chuang Sun Yujia Shi Zhipeng Wang Lianzheng Zhou Qian Zhang Zhengdao Mao Source Type: research
Molecular mechanism of high glucose-induced mitochondrial DNA damage in retinal ganglion cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):219-224. doi: 10.14715/cmb/2024.70.3.33.ABSTRACTMitochondrial DNA damage in retinal ganglion cells (RGCs) may be closely related to lesions of glaucoma. RGCs were cultured with different concentrations of glucose and grouped into 3 groups, namely normal control (NC) group, Low-Glu group, and High-Glu group. Cell viability was measured with cell counting kit-8, and cell apoptosis was measured using flow cytometry. The DNA damage was measured with comet assay, and the morphological changes of damaged mitochondria in RGCs were observed using TEM. Western blot analyzed the expr...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Jinzi Zhou Fenghua Chen Aimin Yan Xiaobo Xia Source Type: research
Research of the unrecognised functions of miR-375 in prostate cancer cells
In conclusion, miR-375 has anti-proliferative and cell migration inhibitory effects in prostate cancer. However, studies demonstrate that miR-375 may have tumor suppressor and oncogenic effects when considering cell molecular differences.PMID:38650131 | DOI:10.14715/cmb/2024.70.3.32 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Merve Goztepe Onur Eroglu Source Type: research
DNMT1 methylation of LncRNA-ANRIL causes myocardial fibrosis pyroptosis by interfering with the NLRP3/Caspase-1 pathway
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):197-203. doi: 10.14715/cmb/2024.70.3.30.ABSTRACTMyocardial fibrosis is a common pathological manifestation that occurs in various cardiac diseases. The present investigation aims to reveal how DNMT1/lncRNA-ANRIL/NLRP3 influences fibrosis and cardiac fibroblast pyroptosis. Here, we used ISO to induce myocardial fibrosis in mice, and LPS and ATP to induce myocardial fibroblast pyroptosis. The results showed that DNMT1, Caspase-1, and NLRP3 expression were significantly increased in fibrotic murine myocardium and pyroptotic cardiac fibroblasts, whereas LncRNA-ANRIL expression ...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Zuntao Liu Lei Gao Chenjing Kan Xin Chen Kaihu Shi Wei Wang Source Type: research
