Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis

This study uses cell suspensions as a platform for expressing the cyt1Aa gene in the coffee genome (C. arabica L. var. Catuaí) to control CBB. The Agrobacterium tumefaciens strain GV3101::pMP90 containing the bar and cyt1Aa genes are used to genetically transform embryogenic cell suspensions. PCR amplification of the cyt1Aa gene is observed 2, 5, and 7 weeks after infection. This chapter describes a protocol that can be used for the development of resistant varieties against biotic and abiotic stresses and CRISPR/Cas9-mediated genome editing.PMID:38656516 | DOI:10.1007/978-1-0716-3782-1_12
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research