Aerobic physical training reduces severe asthma phenotype involving kinins pathway
CONCLUSION: These findings indicate that APT inhibits the severe asthma phenotype by targeting kinin signaling.PMID:38598121 | DOI:10.1007/s11033-024-09474-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Maysa Alves Rodrigues Brandao-Rangel Renilson Moraes-Ferreira Anamei Silva-Reis Victor Hugo Souza-Palmeira Francine Maria Almeida Fabiana Regina da Silva Olimpio Carlos Rocha Oliveira Nilsa Regina Damaceno-Rodrigues Jo ão Bosco Pesquero Leonardo Martin F Source Type: research
Kinesin Regulation in the Proximal Axon is Essential for Dendrite-selective Transport
Mol Biol Cell. 2024 Apr 10:mbcE23110457. doi: 10.1091/mbc.E23-11-0457. Online ahead of print.ABSTRACTNeurons are polarized and typically extend multiple dendrites and one axon. To maintain polarity, vesicles carrying dendritic proteins are arrested upon entering the axon. To determine if kinesin regulation is required for terminating anterograde axonal transport, we overexpressed the dendrite-selective kinesin KIF13A. This caused mistargeting of dendrite-selective vesicles to the axon and a loss of dendritic polarity. Polarity was not disrupted if the kinase MARK2/Par1b was coexpressed. MARK2/Par1b is concentrated in the p...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Christina S Mendoza Cameron R Plowinske Andrew C Montgomery Geraldine B Quinones Gary Banker Marvin Bentley Source Type: research
WHAMM functions in kidney reabsorption and polymerizes actin to promote autophagosomal membrane closure and cargo sequestration
Mol Biol Cell. 2024 Apr 10:mbcE24010025. doi: 10.1091/mbc.E24-01-0025. Online ahead of print.ABSTRACTThe actin cytoskeleton is essential for many functions of eukaryotic cells, but the factors that nucleate actin assembly are not well understood at the organismal level or in the context of disease. To explore the function of the actin nucleation factor WHAMM in mice, we examined how Whamm inactivation impacts kidney physiology and cellular proteostasis. We show that male WHAMM knockout mice excrete elevated levels of albumin, glucose, phosphate, and amino acids, and display structural abnormalities of the kidney proximal t...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Alyssa M Coulter Valerie Cort és Corey J Theodore Rachel E Cianciolo Ron Korstanje Kenneth G Campellone Source Type: research
Bacterial growth dynamics in a rhythmic symbiosis
Mol Biol Cell. 2024 Apr 10:mbcE24010044. doi: 10.1091/mbc.E24-01-0044. Online ahead of print.ABSTRACTThe symbiotic relationship between the bioluminescent bacterium Vibrio fischeri and the bobtail squid Euprymna scolopes serves as a valuable system to investigate bacterial growth and peptidoglycan (PG) synthesis within animal tissues. To better understand the growth dynamics of V. fischeri in the crypts of the light-emitting organ of its juvenile host, we showed that, after the daily dawn-triggered expulsion of most of the population, the remaining symbionts rapidly proliferate for about 6 h. At that point the population e...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Liu Yang Susannah Lawhorn Clotilde Bongrand James C Kosmopoulos Jill Kuwabara Michael VanNieuwenhze Mark J Mandel Margaret McFall-Ngai Edward Ruby Source Type: research
The bistable mitotic switch in fission yeast
Mol Biol Cell. 2024 Apr 10:mbcE24030142. doi: 10.1091/mbc.E24-03-0142. Online ahead of print.ABSTRACTIn favorable conditions, eukaryotic cells proceed irreversibly through the cell division cycle (G1-S-G2-M) in order to produce two daughter cells with the same number and identity of chromosomes of their progenitor. The integrity of this process is maintained by 'checkpoints' that hold a cell at particular transition points of the cycle until all requisite events are completed. The crucial functions of these checkpoints seem to depend on irreversible bistability of the underlying checkpoint control systems. Bistability of c...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: B éla Novák John J Tyson Source Type: research
Antiparallel microtubule bundling supports KIF15-driven mitotic spindle assembly
Mol Biol Cell. 2024 Apr 10:mbcE24010023. doi: 10.1091/mbc.E24-01-0023. Online ahead of print.ABSTRACTThe spindle is a bipolar microtubule-based machine that is crucial for accurate chromosome segregation. Spindle bipolarity is generated by Eg5 (a kinesin-5), a conserved motor that drives spindle assembly by localizing to and sliding apart antiparallel microtubules. In the presence of Eg5 inhibitors (K5Is), KIF15 (a kinesin-12) can promote spindle assembly, resulting in K5I-resistant cells (KIRCs). However, KIF15 is a less potent motor than Eg5, suggesting that other factors may contribute to spindle formation in KIRCs. Pro...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Brittany M Salazar Ryoma Ohi Source Type: research
TGF β overcomes FGF-induced transinhibition of EGFR in lens cells to enable fibrotic secondary cataract
Mol Biol Cell. 2024 Apr 10:mbcE24010040. doi: 10.1091/mbc.E24-01-0040. Online ahead of print.ABSTRACTIn order to cause vision-disrupting fibrotic secondary cataract (PCO), lens epithelial cells that survive cataract surgery must migrate to the posterior of the lens capsule and differentiate into myofibroblasts. During this process, the cells become exposed to the FGF that diffuses out of the vitreous body. In normal development, such relatively high levels of FGF induce lens epithelial cells to differentiate into lens fiber cells. It has been a mystery as to how lens cells could instead undergo a mutually exclusive cell fa...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Judy K VanSlyke Bruce A Boswell Linda S Musil Source Type: research
Neurofilament Biophysics: from Structure to Biomechanics
Mol Biol Cell. 2024 Apr 10:mbcE23110438. doi: 10.1091/mbc.E23-11-0438. Online ahead of print.ABSTRACTNeurofilaments (NFs) are multi-subunit, neuron-specific intermediate filaments consisting of a 10-nm diameter filament "core" surrounded by a layer of long intrinsically disordered protein (IDP) "tails." NFs are thought to regulate axonal caliber during development and then stabilize the mature axon, with NF subunit misregulation, mutation, and aggregation featuring prominently in multiple neurological diseases. The field's understanding of NF structure, mechanics, and function has been deeply informed by a rich variety of ...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Erika A Ding Sanjay Kumar Source Type: research
Scaling of stochastic growth and division dynamics: A comparative study of individual rod-shaped cells in the Mother Machine and SChemostat platforms
Mol Biol Cell. 2024 Apr 10:mbcE23110452. doi: 10.1091/mbc.E23-11-0452. Online ahead of print.ABSTRACTMicrofluidic platforms enable long-term quantification of stochastic behaviors of individual bacterial cells under precisely controlled growth conditions. Yet, quantitative comparisons of physiological parameters and cell behaviors of different microorganisms in different experimental and device modalities is not available due to experiment-specific details affecting cell physiology. To rigorously assess the effects of mechanical confinement, we designed, engineered, and performed side-by-side experiments under otherwise id...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Karl F Ziegler Kunaal Joshi Charles S Wright Shaswata Roy Will Caruso Rudro R Biswas Srividya Iyer-Biswas Source Type: research
N-terminal signals in the SNX-BAR paralogs Vps5 and Vin1 guide endosomal coat complex formation
Mol Biol Cell. 2024 Apr 10:mbcE24010043. doi: 10.1091/mbc.E24-01-0043. Online ahead of print.ABSTRACTEndosomal coats incorporate membrane-binding subunits such as sorting nexin (SNX) proteins. The Saccharomyces cerevisiae SNX-BAR paralogs Vin1 and Vps5 are respective subunits of the endosomal VINE and retromer complexes whose dimerizing BAR domains are required for complex assembly and membrane association. However, a degree of promiscuity is predicted for yeast BAR-BAR pairings, and recent work has implicated the unstructured N-terminal domains of Vin1 and Vps5 in coat formation. Here, we map N-terminal signals in both SN...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Shawn P Shortill Mia S Frier Michael Davey Elizabeth Conibear Source Type: research
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome
Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9. Online ahead of print.ABSTRACTDedicated assembly factors orchestrate the stepwise production of many molecular machines, including the 28-subunit proteasome core particle (CP) that mediates protein degradation. Here we report cryo-electron microscopy reconstructions of seven recombinant human subcomplexes that visualize all five chaperones and the three active site propeptides across a wide swath of the assembly pathway. Comparison of these chaperone-bound intermediates and a matching mature CP reveals molecular mechanisms determining the order of successiv...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Frank Adolf Jiale Du Ellen A Goodall Richard M Walsh Shaun Rawson Susanne von Gronau J Wade Harper John Hanna Brenda A Schulman Source Type: research
Mechanical state transitions in the regulation of tissue form and function
Nat Rev Mol Cell Biol. 2024 Apr 10. doi: 10.1038/s41580-024-00719-x. Online ahead of print.ABSTRACTFrom embryonic development, postnatal growth and adult homeostasis to reparative and disease states, cells and tissues undergo constant changes in genome activity, cell fate, proliferation, movement, metabolism and growth. Importantly, these biological state transitions are coupled to changes in the mechanical and material properties of cells and tissues, termed mechanical state transitions. These mechanical states share features with physical states of matter, liquids and solids. Tissues can switch between mechanical states ...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Yanlan Mao Sara A Wickstr öm Source Type: research
Whole genome sequencing analysis of Komagataeibacter nataicola reveals its potential in food waste valorisation for cellulose production
CONCLUSION: A more comprehensive genome study of K. nataicola may shed light into biological pathway in BC productivity as well as benefit the analysis of metabolites generated and understanding of biological and chemical interactions in BC production later.PMID:38600404 | DOI:10.1007/s11033-024-09492-8 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Muhammad Irhamni Haziqi Nasharudin Shing-Wei Siew Hajar Fauzan Ahmad Nazira Mahmud Source Type: research
Methylated lncRNAs suppress apoptosis of gastric cancer stem cells via the lncRNA-miRNA/protein axis
CONCLUSIONS: Our study revealed the requirement for site-specific methylation of lncRNAs in the tumorigenesis of GCSCs, contributing novel insights into cancer development.PMID:38600465 | PMC:PMC11005211 | DOI:10.1186/s11658-024-00568-8 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Yuan Ci Yuan Zhang Xiaobo Zhang Source Type: research
The Impact of Inflammation on Thermal Hyperpnea: Relevance for Heat Stress and Febrile Seizures
Am J Respir Cell Mol Biol. 2024 Apr 10. doi: 10.1165/rcmb.2023-0451OC. Online ahead of print.ABSTRACTExtreme heat caused by climate change is increasing transmission of infectious diseases resulting in a sharp rise in heat-related illness and mortality. Understanding mechanistic link between heat, inflammation and disease is thus important for public health. Thermal hyperpnea, and consequent respiratory alkalosis is crucial in febrile seizures and convulsions induced by heat stress in humans. Here we address what causes thermal hyperpnea in neonates and how is it affected by inflammation. TRPV1, a heat-activated channel is...
Source: Mol Biol Cell - April 10, 2024 Category: Molecular Biology Authors: Karlene T Barrett Arijit Roy Aya Ebdalla Quentin J Pittman Richard J A Wilson Morris H Scantlebury Source Type: research
