STMND1 is A Phylogenetically Ancient Stathmin which Localizes to Motile Cilia and Exhibits Nuclear Translocation that Is Inhibited When Soluble Tubulin Concentration Increases
Mol Biol Cell. 2024 Apr 17:mbcE23120514. doi: 10.1091/mbc.E23-12-0514. Online ahead of print.ABSTRACTStathmins are small, unstructured proteins that bind tubulin dimers and are implicated in several human diseases, but whose function remains unknown. We characterized a new stathmin, STMND1 (Stathmin Domain Containing 1) as the human representative of an ancient sub-family. STMND1 features a N-terminal myristoylated and palmitoylated motif which directs it to membranes and a tubulin-binding stathmin-like domain (SLD) that contains an internal nuclear localization signal. Biochemistry and proximity labeling showed that STMND...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Xiang Deng Bryan O Seguinot Gary Bradshaw Jong Suk Lee Shannon Coy Marian Kalocsay Sandro Santagata Timothy Mitchison Source Type: research
Impact of two < em > Erwinia < /em > sp. on the response of diverse < em > Pisum sativum < /em > genotypes under salt stress
Physiol Mol Biol Plants. 2024 Feb;30(2):249-267. doi: 10.1007/s12298-024-01419-8. Epub 2024 Feb 25.ABSTRACTCurrently, salinization is impacting more than 50% of arable land, posing a significant challenge to agriculture globally. Salt causes osmotic and ionic stress, determining cell dehydration, ion homeostasis, and metabolic process alteration, thus negatively influencing plant development. A promising sustainable approach to improve plant tolerance to salinity is the use of plant growth-promoting bacteria (PGPB). This work aimed to characterize two bacterial strains, that have been isolated from pea root nodules, initia...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Houda Ilahi Elisa Zampieri Cristiana Sbrana Francesca Brescia Luca Giovannini Roghayyeh Mahmoudi Gholamreza Gohari Mustapha Missbah El Idrissi Mohamed Najib Alfeddy Martino Schillaci Lahcen Ouahmane Alice Calvo Fabiano Sillo Vasileios Fotopoulos Raffaella Source Type: research
Regulation of chloroplast biogenesis, development, and signaling by endogenous and exogenous cues
Physiol Mol Biol Plants. 2024 Feb;30(2):167-183. doi: 10.1007/s12298-024-01427-8. Epub 2024 Mar 11.ABSTRACTChloroplasts are one of the defining features in most plants, primarily known for their unique property to carry out photosynthesis. Besides this, chloroplasts are also associated with hormone and metabolite productions. For this, biogenesis and development of chloroplast are required to be synchronized with the seedling growth to corroborate the maximum rate of photosynthesis following the emergence of seedlings. Chloroplast biogenesis and development are dependent on the signaling to and from the chloroplast, which ...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Kalyan Mahapatra Arpan Mukherjee Shikha Suyal Mansoor Ali Dar Lavanya Bhagavatula Sourav Datta Source Type: research
< em > ALLENE OXIDE SYNTHASE < /em > ( < em > AOS < /em > ) induces petal senescence through a novel JA-associated regulatory pathway in < em > Arabidopsis < /em >
In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in Arabidopsis. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-024-01425-w.PMID:38623171 | PMC:PMC11016053 | DOI:10.1007/s12298-024-01425-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Liuqing Wu Kaiqi Wang Mengyi Chen Wenxin Su Zheng Liu Xiaoying Guo Mengqian Ma Shuangjie Qian Yuqi Deng Haihan Wang Chanjuan Mao Zaibao Zhang Xiaofeng Xu Source Type: research
Lipoic acid attachment to proteins: stimulating new developments
Microbiol Mol Biol Rev. 2024 Apr 16:e0000524. doi: 10.1128/mmbr.00005-24. Online ahead of print.ABSTRACTSUMMARYLipoic acid-modified proteins are essential for central metabolism and pathogenesis. In recent years, the Escherichia coli and Bacillus subtilis lipoyl assembly pathways have been modified and extended to archaea and diverse eukaryotes including humans. These extensions include a new pathway to insert the key sulfur atoms of lipoate, several new pathways of lipoate salvage, and a novel use of lipoic acid in sulfur-oxidizing bacteria. Other advances are the modification of E. coli LplA for studies of protein locali...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: John E Cronan Source Type: research
Current knowledge about FLT3 gene mutations, exploring the isoforms, and protein importance in AML
Mol Biol Rep. 2024 Apr 16;51(1):521. doi: 10.1007/s11033-024-09452-2.ABSTRACTAcute myeloid leukaemia (AML) is a complex haematological malignancy characterised by diverse genetic alterations leading to abnormal proliferation of myeloid precursor cells. One of the most significant genetic alterations in AML involves mutations in the FLT3 gene, which plays a critical role in haematopoiesis and haematopoietic homeostasis. This review explores the current understanding of FLT3 gene mutations and isoforms and the importance of the FLT3 protein in AML. FLT3 mutations, including internal tandem duplications (FLT3-ITD) and point m...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Diana Mace čková Lenka Va ňková Monika Holubov á Pavel Jindra Robin Klieber Eli ška Jandová Pavel Pitule Source Type: research
IGFBP7- When Endothelial gCap-ing Goes Wrong in Acute Lung Injury
Am J Respir Cell Mol Biol. 2024 Apr 16. doi: 10.1165/rcmb.2024-0146ED. Online ahead of print.NO ABSTRACTPMID:38626308 | DOI:10.1165/rcmb.2024-0146ED (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Ranu Surolia Jaroslaw W Zmijewski Source Type: research
GJB2 c.35del variant up-regulates GJA1 gene expression and affects differentiation of human stem cells
Genet Mol Biol. 2024 Apr 15;47(2):e20230170. doi: 10.1590/1678-4685-GMB-2023-0170. eCollection 2024.ABSTRACTPathogenic DNA alterations in GJB2 are present in nearly half of non-syndromic hearing loss cases with autosomal recessive inheritance. The most frequent variant in GJB2 causing non-syndromic hearing loss is the frameshifting c.35del. GJB2 encodes Cx26, a protein of the connexin family that assembles hemichannels and gap junctions. The expression of paralogous proteins is believed to compensate for the loss of function of specific connexins. As Cx26 has been involved in cell differentiation in distinct tissues, we em...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Ana Carla Batissoco Dayane Bernardino Cruz Thiago Geronimo Pires Alegria Gerson Kobayashi Jeanne Oiticica Luis Eduardo Soares Netto Maria Rita Passos-Bueno Luciana Amaral Haddad Regina C élia Mingroni Netto Source Type: research
Investigating the shared genetic architecture between breast and ovarian cancers
Genet Mol Biol. 2024 Apr 15;47(2):e20230181. doi: 10.1590/1678-4685-GMB-2023-0181. eCollection 2024.ABSTRACTHigh heritability and strong correlation have been observed in breast and ovarian cancers. However, their shared genetic architecture remained unclear. Linkage disequilibrium score regression (LDSC) and heritability estimation from summary statistics (ρ-HESS) were applied to estimate heritability and genetic correlations. Bivariate causal mixture model (MiXeR) was used to qualify the polygenic overlap. Then, stratified-LDSC (S-LDSC) was used to identify tissue and cell type specificity. Meanwhile, the adaptive assoc...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Xuezhong Shi Anqi Bu Yongli Yang Yuping Wang Chenyu Zhao Jingwen Fan Chaojun Yang Xiaocan Jia Source Type: research
(Re)-definition of the holo- and apo-Fur direct regulons of Helicobacter pylori
J Mol Biol. 2024 Apr 14:168573. doi: 10.1016/j.jmb.2024.168573. Online ahead of print.ABSTRACTIron homeostasis is a critical process for living organisms because this metal is an essential co-factor for fundamental biochemical activities, like energy production and detoxification, albeit its excess quickly leads to cell intoxication. The protein Fur (ferric uptake regulator) controls iron homeostasis in bacteria by switching from its apo- to holo-form as a function of the cytoplasmic level of ferrous ions, thereby modulating gene expression. The Helicobacter pylori HpFur protein has the rare ability to operate as a transcr...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Andrea Vannini Eva Pinatel Paolo Emidio Costantini Simone Pelliciari Davide Roncarati Simone Puccio Gianluca De Bellis Vincenzo Scarlato Clelia Peano Alberto Danielli Source Type: research
Human umbilical cord mesenchymal stem cell-derived exosomes alleviate the severity of experimental autoimmune encephalomyelitis and enhance lag-3 expression on foxp3 + CD4 + T cells
CONCLUSIONS: These results suggest that hUMSCs-Exs could be a promising anti-inflammatory agent to regulate T-cell responses in EAE and other autoimmune diseases. However, further research is necessary to fully understand the underlying mechanisms and Lag-3's precise role in these conditions.PMID:38627337 | DOI:10.1007/s11033-024-09433-5 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Adel Mohammadzadeh Masoud Lahouty Hamed Charkhian Arash Adamnejad Ghafour Sahand Moazzendizaji Jafar Rezaei Shahriar Alipour Vahid Shafiei Irannejad Mohammad Hassan Khadem Ansari Source Type: research
Golgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b
CONCLUSION: Our results support that Rab4b, by controlling the sorting of the cation-independent mannose-6-phosphate receptor towards VPS52 microdomains, confers a directional specificity for cargo carriers en route to the trans-Golgi network. Given the importance of the endocytic recycling in cell homeostasis, disruption of the Rab4b/Golgi-associated retrograde protein complex-dependent step could have serious consequences in pathologies.PMID:38627612 | DOI:10.1186/s11658-024-00574-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: J érôme Gilleron Abderrahman Chafik Sandra Lacas-Gervais Jean-Fran çois Tanti Mireille Cormont Source Type: research
Evaluation of the Genetic Background of Patients with Niemann-Pick Disease
CONCLUSIONS: In this study, disease-causing variants were identified in three probands suspected of Niemann-Pick disease. Such results show the relatively high power of molecular techniques to assist clinicians with disease management, therapeutic strategies, and preventive options such as preimplantation genetic diagnosis and prenatal diagnosis.PMID:38618260 | PMC:PMC11015928 | DOI:10.61186/rbmb.12.3.386 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 15, 2024 Category: Molecular Biology Authors: Fatemeh Alipouran Ehsan Ghayoor Karimiani Jina Khayatzadeh Source Type: research
Resistance Improvement and Sensitivity Enhancement of Cancer Therapy by a Novel Antitumor Candidate onto A2780 CP and A2780 S Cell Lines
CONCLUSIONS: As a result, PBPD is a promising anticancer agent for CDDP-resistant ovarian cancer.PMID:38618266 | PMC:PMC11015932 | DOI:10.61186/rbmb.12.3.374 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 15, 2024 Category: Molecular Biology Authors: Sariyeh Mohammadi Hadloo Homa Mohseni Kouchesfahani Ali Khanlarkhani Maryam Saeidifar Source Type: research
The Role of Vitamin D Binding Protein and Vitamin D Level in Mortality of Sepsis Patients
CONCLUSIONS: Diminished levels of vitamin D binding protein and vitamin D contribute to an increased risk of mortality in septic patients.PMID:38618268 | PMC:PMC11015934 | DOI:10.61186/rbmb.12.3.366 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 15, 2024 Category: Molecular Biology Authors: Liliriawati Ananta Kahar Yusrawati Yusrawati Jamsari Jamsari Tinni Maskoen Kornelis Aribowo Wiwi Monika Sari Source Type: research
