Chelerythrine induces apoptosis and ferroptosis through Nrf2 in ovarian cancer cells
In this study, we investigated the cytotoxic mechanism and potential targets of CHE on in vitro cultures of A2780 and SKOV3 cells derived from ovarian cancer. Additionally, in vivo experiments were conducted to confirm the suppressive impact of CHE on tumor growth in nude mice. The findings revealed that CHE impeded the growth of A2780 and SKOV3 cells in a concentration-time-dependent manner and significantly suppressed the development of tumors in nude mice. CHE elevated the level of oxidative stress in tumor cells, prompted cell cycle halt in the S phase, and increased their mitochondrial membrane potential. Western blot...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Jia Zhou Yuchen Wang Yangxin Fu Zhe Lin He Lin Guangfu Lv Ning Kong Guo Xin Fang Zhou Zhidong Qiu Xiaowei Huang Source Type: research
Lactoferrin mediates epithelial-mesenchymal transformation by regulating the PI3K/AKT/mTOR pathway to inhibit nasopharyngeal carcinoma metastasis
This study suggests that LTF is a potential biomarker of NPC and that LTF-mediated EMT progression plays a tumor-suppressive role in the progression of NPC metastasis.PMID:38650146 | DOI:10.14715/cmb/2024.70.3.19 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Min Xu Ye Fan Guoying Zou Qi Yang Fei Xu Source Type: research
Development of a novel six DNA damage response-related prognostic signature in osteosarcoma
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):110-115. doi: 10.14715/cmb/2024.70.3.16.ABSTRACTDNA damage response (DDR) plays a vital role in the development of cancer. Nevertheless, in osteosarcoma, the potential of DDR-related genes (DDRGs) remains unclear. Thus, the current research is intended to investigate the mechanisms of DDRGs in the development of osteosarcoma and to explore potential DDR-related biomarkers in forecasting the prognosis of osteosarcoma patients. The osteosarcoma genomic data from TCGA, GEO and cBioPortal databases were utilized for screening and identification of differentially expressed DDRGs...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Tianyang Li Zhenzhou Tang Sucheng Li Minhua Lu Source Type: research
TNFRSF6 induces mitochondrial dysfunction and microglia activation in the in vivo and in vitro models of sepsis-associated encephalopathy
This study aimed to explore TNFRSF6 function in SAE. The SAE mouse model was established by intraperitoneal injection of LPS in TNFRSF6-/- mice and C57BL/6J mice. Microglia were treated with LPS to establish the cell model. The learning, memory and cognitive functions in mice were tested by behavioral tests. Nissl staining was utilized for determining neuronal injury. Microglial activation was tested by immunofluorescence assay. ELISA was utilized for determining TNF-α, IL-1β, IL-6, and IL-10 contents. Mitochondrial dysfunction was measured by mitochondrial oxygen consumption, ATP content, ROS production, and JC-1 assay....
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Danfeng Yu Yanmei Ji Jing Zhang Xiaochuan Huang Source Type: research
Ginsenoside Rc alleviates osteoporosis by the TGF- β/Smad signaling pathway
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):95-101. doi: 10.14715/cmb/2024.70.3.14.ABSTRACTOsteoporosis is a common chronic bone disorder in postmenopausal women. Ginsenosides are primary active components in ginseng and the effects of various ginsenoside variants in osteoporosis treatment have been widely revealed. We planned to explore the impact of ginsenoside Rc on bone resorption in an osteoporosis rat model. We used ovariectomized rats to assess the potential impact of ginsenoside Rc on osteoporosis. μ-CT was implemented for analyzing the microstructure of the distal left femur in rats. H&E staining togeth...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Shanfu Wang Bing Xu Heng Yin Zhen Hua Yang Shao Jianwei Wang Source Type: research
Identification of osteogenic differentiation-related miRNA-mRNA regulatory network of human periodontal ligament stem cells
This study aimed to investigate the hub genes and miRNA-mRNA regulatory network around periodontal ligament stem cells (PDLSC) for osteogenic differentiation through bioinformatic analysis. The dataset with osteogenic differentiation of human PDLSC was downloaded from the GEO database. The Weighted gene coexpression network analysis (WGCNA) was performed to identify key modules and hub genes. In addition, differentially expressed genes (DEGs) analysis was conducted with limma. The functional enrichment of differentially expressed hub genes was implemented with KEGG and GSEA analysis. The targeted genes of differentially ex...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Zhenrong Chen Lulu Long Mianjia Wan Min Huang Haijuan Wu Qun Huang Source Type: research
Identification of 10 differentially expressed and cuproptosis-related genes in immune infiltration and prognosis of thyroid carcinoma
We presented the expression profiles of dysregulated genes in thyroid carcinoma. The findings of our study highlighted the potential of cuproptosis-related genes as prognostic biomarkers for thyroid carcinoma.PMID:38650151 | DOI:10.14715/cmb/2024.70.3.13 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Weixuan Yu Haiping Liu Yunda Zhang Ming Liu Weicong Li Libin Wang Dongwei Li Source Type: research
The relationship between preeclampsia risk and SENCR rs555172 gene polymorphism and expression
In conclusion, the LncRNA SENCR rs555172(G/A) seems not associated with an increased risk of Preeclampsia in pregnant women.PMID:38650152 | DOI:10.14715/cmb/2024.70.3.11 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Mohsen Saravani Elham Kazemi Hasan Dana Sepehr Kahrizi Roya Zanganeh Hamidreza Chegini Sodabe Rezaei Marzieh Ghasemi Majid Zaki-Dizaji Mostafa Saeedinia Zohreh Heidary Source Type: research
Antibacterial effect and biocompatibility of silver nanoparticle-coated bone allograft substitutes
This study aimed to coat bone allografts with silver nanoparticles and assess their antibacterial activity and biocompatibility compared to uncoated bone allografts. In this study, the bone allografts were coated with varying concentrations of silver nanoparticles (5 mg/l, 10 mg/l, and 50 mg/l) through a simple adsorption technique. Subsequently, the coated samples underwent characterization using SEM, FTIR, EDS, and XRD. The Minimal Inhibitory Concentration (MIC) of the silver nanoparticles was determined against Staphylococcus aureus and Streptococcus mutans. Bacterial growth inhibition was evaluated by measuring turbidi...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Sanna Hadi Othman Omar Source Type: research
MicroRNA-503-5p protects streptozotocin-induced erectile dysfunction in diabetic rats by downregulating SYDE2
This study aimed to figure out microRNA (miR)-503-5p/SYDE2 axis' latent mechanisms in streptozotocin-induced diabetic rat sexual dysfunction. A model of erectile dysfunction (ED) in diabetic rats was established by injecting streptozotocin. MiR-503-5p and SYDE2 in ED rats were altered by injection of miR-503-5p mimic or si/oe-SYDE2. The targeting link between miR-503-5p and SYDE2 was testified. ICP/MAP value was tested by pressure sensor; Penile capillary abundance was assessed; Penile cGMP and AGEs were detected; penile smooth muscle cell apoptosis was assessed; MiR-503-5p and SYDE2 were tested. In streptozotocin-induced ...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Gangqin Li Lin Jiang Kuan Bai Guangxing Tan Source Type: research
Identification of hub genes and key pathways in spinal cord injury via bioinformatics analysis
This study aimed to explore the hub genes and related key pathways in Spinal Cord Injury (SCI) based on the bioinformatics analysis. Two microarray datasets (GSE45006, GSE45550) were obtained from the GEO database and were merged and batch-corrected. The differentially expressed genes (DEGs) in SCI were explored with the Limma, and the weighted gene co-expression network analysis (WGCNA) was conducted to explore the module genes. Functional enrichment analysis and Gene set variation analysis (GSVA) were used to investigate the biological functions and key pathways of the key genes related to SCI. Then the protein-protein i...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Hao Cheng Yanben Wang Jun Xuan Source Type: research
MiR-4319 targets tuftelin 1 to reduce malignancy of cervical cancer cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):54-60. doi: 10.14715/cmb/2024.70.3.8.ABSTRACTCervical cancer (CC) is the most common malignant tumor of female reproductive system. MiR-4319 has been identified as an anti-oncogene in various cancers. In the present study, role of miR-4319 in CC was identified. Colony formation, flow cytometer, wound healing, and transwell assays were used to detect CC cell proliferation, apoptosis, migration, and invasion. The expression of miR-4319 was decreased in clinical CC tissues and CC cell lines. Upregulation of miR-4319 suppressed cell viability, proliferation, migration, and inva...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Lijun Zheng Qiongzhen Ren Weipei Zhu Xiaomin Tao Liangsheng Guo Source Type: research
Identification of small molecule inhibitors of penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus for the therapeutics of bacterial infection
In conclusion, the results suggest alpha-mangostin as a promising candidate for inhibiting PBP2a. Further, extensive studies are warranted to explore its clinical applications.PMID:38650157 | DOI:10.14715/cmb/2024.70.3.6 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Anwer Razique Source Type: research
Clinical value of KiSS-1 and MMP-2 expression levels in breast cancer tissue in evaluating prognosis of elderly breast cancer patients after modified radical mastectomy
In conclusion, KiSS-1 and MMP-2 levels in BC tissue possess relation to adverse prognosis of MCM. KiSS-1 and MMP-2 levels in elderly BC patients before surgery may be detected in the future to assist in prognosis evaluation of elderly BC patients after MCM.PMID:38650158 | DOI:10.14715/cmb/2024.70.3.38 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Jiyang Lv Junya Zhang Fangfang Wu Source Type: research
In vivo determination of the anti-inflammatory and antioxidant effects of the aqueous extract of Syzygium aromaticum (clove) in an asthmatic rat model
This study aimed to evaluate the anti-inflammatory and antioxidant effects of an Aqueous Extract of Clove (AEC) Syzygium aromaticum on the lungs and erythrocytes of an experimental asthma model in Wistar rats. For this purpose, four groups of male rats were examined: control, sensitized with ovalbumin (OVA), treated with AEC, and treated with a combination of OVA/AEC. After treatment, the antioxidant effect was determined by measuring the malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione (GSH), and catalase (CAT) levels. The anti-inflammatory effect was determined by measuring IL-4 levels by performing enzym...
Source: Mol Biol Cell - April 23, 2024 Category: Molecular Biology Authors: Lynda Sabrina Ounaceur Mahfoud Messarah Abdelaziz Lankar Djihane Touaibia Hanene Ghadab Zohir Garri Sonia Boudjil Khadidja Belkacem Djeffel Latifa Atoui Anis Ounaceur Nesrine Djaber Amel Boumendjel Source Type: research
