Good manufacturing practices production of human placental derived mesenchymal stem cells for therapeutic applications: focus on multiple sclerosis
CONCLUSIONS: We hope that our protocol for clinical manufacturing of PLMSCs according to GMP standards will assist researchers in isolating MSCs from placental tissue for clinical and pre-clinical applications.PMID:38551770 | DOI:10.1007/s11033-024-09372-1 (Source: Mol Biol Cell)
Source: Mol Biol Cell - March 29, 2024 Category: Molecular Biology Authors: Ameneh Shokati Abdorreza Naser Moghadasi Andisheh Ghashghaei Mohammad Ali Sahraian Bahram Chahardouli Seyed Asadollah Mousavi Jafar Ai Mohsen Nikbakht Source Type: research
Minocycline induced apoptosis and suppressed expression of matrix metalloproteinases 2 and 9 in the breast cancer MCF-7 cells
CONCLUSION: These findings further confirmed the anticancer effect of minocycline and highlighted that minocycline maybe considered as potential therapeutic agent for breast cancer treatment.PMID:38551800 | DOI:10.1007/s11033-024-09380-1 (Source: Mol Biol Cell)
Source: Mol Biol Cell - March 29, 2024 Category: Molecular Biology Authors: Abedeh Rezaei Amin Moqadami Mohammad Khalaj-Kondori Mohammad Ali Hosseinpour Feizi Source Type: research
Multiomics Analyses Provide New Insight into Genetic Variation of Reproductive Adaptability in Tibetan Sheep
Mol Biol Evol. 2024 Mar 1;41(3):msae058. doi: 10.1093/molbev/msae058.ABSTRACTDomestication and artificial selection during production-oriented breeding have greatly shaped the level of genomic variability in sheep. However, the genetic variation associated with increased reproduction remains elusive. Here, two groups of samples from consecutively monotocous and polytocous sheep were collected for genome-wide association, transcriptomic, proteomic, and metabolomic analyses to explore the genetic variation in fecundity in Tibetan sheep. Genome-wide association study revealed strong associations between BMPR1B (p.Q249R) and l...
Source: Mol Biol Cell - March 29, 2024 Category: Molecular Biology Authors: Buying Han Dehong Tian Xue Li Sijia Liu Fei Tian Dehui Liu Song Wang Kai Zhao Source Type: research
Interaction of 3,9-disubstituted acridine with single stranded poly(rA), double stranded poly(rAU) and triple stranded poly(rUAU): molecular docking - A spectroscopic tandem study
Chem Biol Interact. 2024 Mar 27:110965. doi: 10.1016/j.cbi.2024.110965. Online ahead of print.ABSTRACTRNA plays an important role in many biological processes which are crucial for cell survival, and it has been suggested that it may be possible to inhibit individual processes involved in many diseases by targeting specific sequences of RNA. The aim of this work is to determine the affinity of novel 3,9-disubstited acridine derivative 1 with three different RNA molecules, namely single stranded poly(rA), double stranded homopolymer poly(rAU) and triple stranded poly(rUAU). The results of the absorption titration assays sho...
Source: Mol Biol Cell - March 29, 2024 Category: Molecular Biology Authors: Krist ína Krochtová Ladislav Janovec Vikt ória Bogárová Annam ária Halečková M ária Kožurková Source Type: research
Identification of immunity-related genes distinctly regulated by Manduca sexta Sp ӓtzle-1/2 and Escherichia coli peptidoglycan
In this study, we produced M. sexta proSpätzle-1 and proSpätzle-2 in Sf9 cells, identified their processing enzymes, and used prophenoloxidase activating protease-3 to activate the cytokine precursors. After Spätzle-1 and -2 were isolated from the reaction mixtures, we separately injected the purified cytokines into larval hemocoel to induce gene transcription in fat body through the Toll pathway solely. On the other hand, we treated a M. sexta cell line with E. coli DAP-PG to only induce the Imd pathway and target gene expression. RNA-Seq analysis of the fat body and cultured cells collected at 0, 6, and 24 h after tre...
Source: Mol Biol Cell - March 29, 2024 Category: Molecular Biology Authors: Zelong Miao Chao Xiong Yang Wang Tisheng Shan Haobo Jiang Source Type: research
