Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography
Methods Mol Biol. 2024;2798:131-139. doi: 10.1007/978-1-0716-3826-2_8.ABSTRACTThe current concepts emphasize the fundamental role of reactive oxygen species (ROS) as signaling molecules that coordinate defense mechanisms, cell death, and the growth and development processes in plants. However, due to the inherent reactivity of ROS, achieving precise control over their levels within plant cells, both spatially and temporally, becomes important to effectively harness the potential of ROS signaling while concurrently minimizing the risk of oxidative damage. Ascorbate is an exceptional antioxidant and contributes to the antiox...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Tamami Hamada Takanori Maruta Source Type: research
Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues
Methods Mol Biol. 2024;2798:205-212. doi: 10.1007/978-1-0716-3826-2_14.ABSTRACTSuperoxide and hydrogen peroxide are reactive oxygen species (ROS) involved in the oxidation of multiple biological molecules and the signaling processes during plant growth and stress response. Thus, control of ROS is fundamental for cell survival and development, with superoxide dismutase (EC 1.15.1.1, SOD) being one of the main enzymes involved. Different isoforms of SOD catalyze the dismutation of superoxide (O2.-) to hydrogen peroxide (H2O2) and oxygen (O2), such as Mn-SODs, Cu,Zn-SODs, and Fe-SODs. Using non-denaturing polyacrylamide gel e...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Ana Jim énez Sandra Correa Francisca Sevilla Source Type: research
DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling
This study aims to elucidate the role and underlying mechanism of DACH1 in airway inflammation of COPD. DACH1 expression was measured in lung tissues of patients with COPD. Airway epithelium-specific DACH1 knockdown mice and AAV-transfected DACH1 overexpressed mice were used to investigate its role and potential for therapeutic targeting in experimental COPD caused by CS. Furthermore, we discovered a potential mechanism of DACH1 in inflammation induced by cigarette smoke extract simulation (CSE) in vitro. Compared to non-smokers and smokers without COPD, COPD patients had reduced DACH1 expression, especially in the airway ...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Qian Huang Yiya Gu Jixing Wu Yuan Zhan Zhesong Deng Shanshan Chen Maocuo Peng Ruonan Yang Jinkun Chen Jungang Xie Source Type: research
Inhibition of GLUD1 mediated by LASP1 and SYVN1 contributes to hepatitis B virus X protein-induced hepatocarcinogenesis
J Mol Cell Biol. 2024 Apr 8:mjae014. doi: 10.1093/jmcb/mjae014. Online ahead of print.ABSTRACTGlutamate dehydrogenase 1 (GLUD1) is implicated in oncogenesis. However, little is known about the relationship between GLUD1 and hepatocellular carcinoma (HCC). In the present study, we demonstrated that the expression levels of GLUD1 significantly decreased in tumors, which was relevant to the poor prognosis of HCC. Functionally, GLUD1 silencing enhanced the growth and migration of HCC cells. Mechanistically, the upregulation of interleukin-32 through AKT activation contributes to GLUD1 silencing-facilitated hepatocarcinogenesis...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Hong-Juan You Qi Li Li-Hong Ma Xing Wang Huan-Yang Zhang Yu-Xin Wang En-Si Bao Yu-Jie Zhong De-Long Kong Xiang-Ye Liu Fan-Yun Kong Kui-Yang Zheng Ren-Xian Tang Source Type: research
Structure of adenylyl cyclase 5 in complex with G βγ offers insights into ADCY5-related dyskinesia
Nat Struct Mol Biol. 2024 Apr 8. doi: 10.1038/s41594-024-01263-0. Online ahead of print.ABSTRACTThe nine different membrane-anchored adenylyl cyclase isoforms (AC1-9) in mammals are stimulated by the heterotrimeric G protein, Gαs, but their response to Gβγ regulation is isoform specific. In the present study, we report cryo-electron microscope structures of ligand-free AC5 in complex with Gβγ and a dimeric form of AC5 that could be involved in its regulation. Gβγ binds to a coiled-coil domain that links the AC transmembrane region to its catalytic core as well as to a region (C1b) that is known to be a hub for isofo...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Yu-Chen Yen Yong Li Chun-Liang Chen Thomas Klose Val J Watts Carmen W Dessauer John J G Tesmer Source Type: research
A novel photosystem assembly line worker
Nat Rev Mol Cell Biol. 2024 Apr 8. doi: 10.1038/s41580-024-00732-0. Online ahead of print.NO ABSTRACTPMID:38589639 | DOI:10.1038/s41580-024-00732-0 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Lisa Heinke Source Type: research
Fibroblast and myofibroblast activation in normal tissue repair and fibrosis
Nat Rev Mol Cell Biol. 2024 Apr 8. doi: 10.1038/s41580-024-00716-0. Online ahead of print.ABSTRACTThe term 'fibroblast' often serves as a catch-all for a diverse array of mesenchymal cells, including perivascular cells, stromal progenitor cells and bona fide fibroblasts. Although phenotypically similar, these subpopulations are functionally distinct, maintaining tissue integrity and serving as local progenitor reservoirs. In response to tissue injury, these cells undergo a dynamic fibroblast-myofibroblast transition, marked by extracellular matrix secretion and contraction of actomyosin-based stress fibres. Importantly, wh...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Fereshteh Sadat Younesi Andrew E Miller Thomas H Barker Fabio M V Rossi Boris Hinz Source Type: research
Escaping from CRISPR-Cas-mediated knockout: the facts, mechanisms, and applications
Cell Mol Biol Lett. 2024 Apr 8;29(1):48. doi: 10.1186/s11658-024-00565-x.ABSTRACTClustered regularly interspaced short palindromic repeats and associated Cas protein (CRISPR-Cas), a powerful genome editing tool, has revolutionized gene function investigation and exhibits huge potential for clinical applications. CRISPR-Cas-mediated gene knockout has already become a routine method in research laboratories. However, in the last few years, accumulating evidences have demonstrated that genes knocked out by CRISPR-Cas may not be truly silenced. Functional residual proteins could be generated in such knockout organisms to compe...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Ying Wang Yujing Zhai Mingzhe Zhang Chunlin Song Yuqing Zhang Gang Zhang Source Type: research
G6PD maintains the VSMC synthetic phenotype and accelerates vascular neointimal hyperplasia by inhibiting the VDAC1-Bax-mediated mitochondrial apoptosis pathway
CONCLUSION: Our study showed that the G6PD-VDAC1-Bax axis is a vital switch in VSMC apoptosis and is essential for VSMC phenotypic switching and neointimal hyperplasia, providing mechanistic insight into early VRDs.PMID:38589823 | DOI:10.1186/s11658-024-00566-w (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Ting Zhang Rui-Jie Cao Jiang-Ling Niu Zhi-Huan Chen Shi-Qing Mu Tong Cao Jie-Xin Pang Li-Hua Dong Source Type: research
HIV-1 integrase assembles multiple species of stable synaptic complex intasomes that are active for concerted DNA integration in vitro
J Mol Biol. 2024 Apr 4:168557. doi: 10.1016/j.jmb.2024.168557. Online ahead of print.ABSTRACTRetroviral DNA integration is mediated by nucleoprotein complexes (intasomes) in which a pair of viral DNA ends are bridged by a multimer of integrase (IN). Most of the high-resolution structures of HIV-1 intasomes are based on an HIV-1 IN with an Sso7d protein domain fused to the N-terminus. Sso7d-IN aggregates much less than wild-type IN and has been critical for structural studies of HIV-1 intasomes. Unexpectedly, these structures revealed that the common core architecture that mediates catalysis could be assembled in various wa...
Source: Mol Biol Cell - April 6, 2024 Category: Molecular Biology Authors: Min Li Renbin Yang Xuemin Chen Huaibin Wang Rodolfo Ghirlando Emilios K Dimitriadis Robert Craigie Source Type: research
HIV-1 integrase assembles multiple species of stable synaptic complex intasomes that are active for concerted DNA integration in vitro
J Mol Biol. 2024 Apr 4:168557. doi: 10.1016/j.jmb.2024.168557. Online ahead of print.ABSTRACTRetroviral DNA integration is mediated by nucleoprotein complexes (intasomes) in which a pair of viral DNA ends are bridged by a multimer of integrase (IN). Most of the high-resolution structures of HIV-1 intasomes are based on an HIV-1 IN with an Sso7d protein domain fused to the N-terminus. Sso7d-IN aggregates much less than wild-type IN and has been critical for structural studies of HIV-1 intasomes. Unexpectedly, these structures revealed that the common core architecture that mediates catalysis could be assembled in various wa...
Source: Mol Biol Cell - April 6, 2024 Category: Molecular Biology Authors: Min Li Renbin Yang Xuemin Chen Huaibin Wang Rodolfo Ghirlando Emilios K Dimitriadis Robert Craigie Source Type: research
LTBP2 regulates cisplatin resistance in GC cells via activation of the NF- κB2/BCL3 pathway
In conclusion, silencing LTBP2 could effectively inhibit cell proliferation and mitigate cisplatin resistance via the NFKB noncanonical pathway NFKB2 p52/Bcl-3/cyclin D1. These findings propose LTBP2 as a potential therapeutic target for overcoming cisplatin resistance in GC patients.PMID:38577985 | PMC:PMC10995769 | DOI:10.1590/1678-4685-GMB-2023-0231 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Jun Wang Wenjia Liang Xiangwen Wang Zhao Chen Lei Jiang Source Type: research
Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells
CONCLUSIONS: Collectively, short-term regulation of TSFM level in cultured cells does not cause a significant change in proteins involved in APP processing, levels in ROS and ATP associated with mitochondrial function. Whereas our study could contribute to comprehend certain clinical features of TSFM mutations, the roles of TSFM in cardiomyopathy and cancer development might deserve further investigation.PMID:38578353 | DOI:10.1007/s11033-024-09426-4 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Xiao-Yun Li Gui-Feng Zhou Xiong-Yong Xie Ya-Lan Pu Xue -Chen Chen-Lu Li Jie Yang Lu Wang Guo-Jun Chen Source Type: research
Bovine lactoferrin inhibits inflammatory response and apoptosis in lipopolysaccharide-induced acute lung injury by targeting the PPAR- γ pathway
CONCLUSION: This study has suggested that the PPAR-γ pathway is the critical target of bLF in anti-inflammatory reactions and apoptosis of ALI, which provides a direction for further research.PMID:38578368 | DOI:10.1007/s11033-024-09436-2 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Yantao Li Junhu Li Yan Dong Can Wang Zhigang Cai Source Type: research
Anti-cancer effects of alpha lipoic acid, cisplatin and paclitaxel combination in the OVCAR-3 ovarian adenocarcinoma cell line
CONCLUSIONS: This study is the first one to investigate the combined treatment of ALA, Cisplatin, Paclitaxel on OVCAR-3. While ALA alone was not effective, combined therapy with ALA, has been found to reduce cell invasion, especially wound healing in the first 24 h, along with tumor cell adhesion.PMID:38578399 | DOI:10.1007/s11033-024-09422-8 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Hatice Şiyzen Çoban Nazl ı Çil Elif Önder G ülçin Abban Mete Source Type: research
