Creation of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel Derived from a Size-Sorted Diploid Clone
Methods Mol Biol. 2024;2797:323-336. doi: 10.1007/978-1-0716-3822-4_23.ABSTRACTCell line panels have proven to be an invaluable tool for investigators researching a range of topics from drug mechanism or drug sensitivity studies to disease-specific etiology. The cell lines used in these panels may range from heterogeneous tumor populations grown from primary tumor isolations to genetically engineered clonal cell lines which express specific gene isoforms. Mouse embryonic fibroblast (MEF) cells are a commonly used cell line for biological research due to their accessibility and ease of genetic manipulation. This chapter wil...
Source: Mol Biol Cell - April 3, 2024 Category: Molecular Biology Authors: William Burgan Source Type: research
Quality Control of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel
Methods Mol Biol. 2024;2797:337-350. doi: 10.1007/978-1-0716-3822-4_24.ABSTRACTIsogenic H/N/KRAS-less mouse embryonic fibroblast (MEF) cell lines have been developed to assist in cell-based assays of RAS inhibitors. The quality control assessment of a panel of these isogenic MEFs is described here, with a focus on ensuring the proper insertion of the desired mutant RAS transgene, a determination of gene copy number, and an investigation of potential off-target mutations which could lead to phenotypes which are undesired in downstream experiments. Using this suite of quality control tools, a MEF cell line can be readily val...
Source: Mol Biol Cell - April 3, 2024 Category: Molecular Biology Authors: Bingfang Xu Katie Powell Source Type: research
A Facile Method to Append a Bio-ID Tag to Endogenous Mutant Kras Alleles
Methods Mol Biol. 2024;2797:351-362. doi: 10.1007/978-1-0716-3822-4_25.ABSTRACTKRAS mutations occur in approximately ~50% of colorectal cancers (CRCs) and are associated with poor prognosis and resistance to therapy. While these most common mutations found at amino acids G12, G13, Q61, and A146 have long been considered oncogenic drivers of CRC, emerging clinical data suggest that each mutation may possess different biological functions, resulting in varying consequences in oncogenesis. Currently, the mechanistic underpinnings associated with each allelic variation remain unclear. Elucidating the unique effectors of each K...
Source: Mol Biol Cell - April 3, 2024 Category: Molecular Biology Authors: Konstantin Budagyan Alexa C Cannon Jonathan Chernoff Source Type: research
Sialylation on vesicular integrin β1 determined endocytic entry of small extracellular vesicles into recipient cells
CONCLUSIONS: Taken together, our findings indicate important functional roles of sialic acids in sEV uptake and reprogramming plasticity of surrounding normal epithelial cells.PMID:38561669 | PMC:PMC10983696 | DOI:10.1186/s11658-024-00562-0 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Meixuan Lin Xiaoqiang Xu Xiaoman Zhou Hui Feng Ruili Wang Yunyun Yang Jing Li Ning Fan Yazhuo Jiang Xiang Li Feng Guan Zengqi Tan Source Type: research
Tracing the lipidome in inborn errors of metabolism
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 31:159491. doi: 10.1016/j.bbalip.2024.159491. Online ahead of print.ABSTRACTInborn errors of metabolism (IEM) represent a heterogeneous group of more than 1800 rare disorders, many of which are causing significant childhood morbidity and mortality. More than 100 IEM are linked to dyslipidaemia, but yet our knowledge in connecting genetic information with lipidomic data is limited. Stable isotope tracing studies of the lipid metabolism (STL) provide insights on the dynamic of cellular lipid processes and could thereby facilitate the delineation of underlying metabolic (pat...
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Martina Zandl-Lang Source Type: research
Opportunities and challenges in design and optimization of protein function
Nat Rev Mol Cell Biol. 2024 Apr 2. doi: 10.1038/s41580-024-00718-y. Online ahead of print.ABSTRACTThe field of protein design has made remarkable progress over the past decade. Historically, the low reliability of purely structure-based design methods limited their application, but recent strategies that combine structure-based and sequence-based calculations, as well as machine learning tools, have dramatically improved protein engineering and design. In this Review, we discuss how these methods have enabled the design of increasingly complex structures and therapeutically relevant activities. Additionally, protein optimi...
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Dina Listov Casper A Goverde Bruno E Correia Sarel Jacob Fleishman Source Type: research
Sialylation on vesicular integrin β1 determined endocytic entry of small extracellular vesicles into recipient cells
CONCLUSIONS: Taken together, our findings indicate important functional roles of sialic acids in sEV uptake and reprogramming plasticity of surrounding normal epithelial cells.PMID:38561669 | DOI:10.1186/s11658-024-00562-0 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Meixuan Lin Xiaoqiang Xu Xiaoman Zhou Hui Feng Ruili Wang Yunyun Yang Jing Li Ning Fan Yazhuo Jiang Xiang Li Feng Guan Zengqi Tan Source Type: research
Tracing the lipidome in inborn errors of metabolism
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 31:159491. doi: 10.1016/j.bbalip.2024.159491. Online ahead of print.ABSTRACTInborn errors of metabolism (IEM) represent a heterogeneous group of more than 1800 rare disorders, many of which are causing significant childhood morbidity and mortality. More than 100 IEM are linked to dyslipidaemia, but yet our knowledge in connecting genetic information with lipidomic data is limited. Stable isotope tracing studies of the lipid metabolism (STL) provide insights on the dynamic of cellular lipid processes and could thereby facilitate the delineation of underlying metabolic (pat...
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Martina Zandl-Lang Source Type: research
Opportunities and challenges in design and optimization of protein function
Nat Rev Mol Cell Biol. 2024 Apr 2. doi: 10.1038/s41580-024-00718-y. Online ahead of print.ABSTRACTThe field of protein design has made remarkable progress over the past decade. Historically, the low reliability of purely structure-based design methods limited their application, but recent strategies that combine structure-based and sequence-based calculations, as well as machine learning tools, have dramatically improved protein engineering and design. In this Review, we discuss how these methods have enabled the design of increasingly complex structures and therapeutically relevant activities. Additionally, protein optimi...
Source: Mol Biol Cell - April 2, 2024 Category: Molecular Biology Authors: Dina Listov Casper A Goverde Bruno E Correia Sarel Jacob Fleishman Source Type: research
Airway Proteotypes of E-Cigarette Users Overlap with Those Found in Asthmatics
Am J Respir Cell Mol Biol. 2024 Apr;70(4):326-328. doi: 10.1165/rcmb.2023-0381LE.NO ABSTRACTPMID:38557396 | DOI:10.1165/rcmb.2023-0381LE (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 1, 2024 Category: Molecular Biology Authors: Elise Hickman Neil E Alexis Julia E Rager Ilona Jaspers Source Type: research
April Highlights/Papers by Junior Investigators/NIH News
Am J Respir Cell Mol Biol. 2024 Apr;70(4):iii-iv. doi: 10.1165/rcmb.70i4RedAlert.NO ABSTRACTPMID:38557397 | DOI:10.1165/rcmb.70i4RedAlert (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 1, 2024 Category: Molecular Biology Source Type: research
Airway Proteotypes of E-Cigarette Users Overlap with Those Found in Asthmatics
Am J Respir Cell Mol Biol. 2024 Apr;70(4):326-328. doi: 10.1165/rcmb.2023-0381LE.NO ABSTRACTPMID:38557396 | DOI:10.1165/rcmb.2023-0381LE (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 1, 2024 Category: Molecular Biology Authors: Elise Hickman Neil E Alexis Julia E Rager Ilona Jaspers Source Type: research
April Highlights/Papers by Junior Investigators/NIH News
Am J Respir Cell Mol Biol. 2024 Apr;70(4):iii-iv. doi: 10.1165/rcmb.70i4RedAlert.NO ABSTRACTPMID:38557397 | DOI:10.1165/rcmb.70i4RedAlert (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 1, 2024 Category: Molecular Biology Source Type: research
Ataxia telangiectasia and Rad3-related (ATR) inhibition by VE-822 potently reversed 5-flourouracil resistance in colorectal cancer cells through targeting DNA damage response
CONCLUSION: The simultaneous treatment of resistant colorectal cancer cells with 5-FU and ATR inhibitor, VE-822, was demonstrated to be effective in reversing drug resistance and potentiating 5-FU mediated anticancer effects via targeting DNA damage.PMID:38553623 | DOI:10.1007/s11033-024-09431-7 (Source: Mol Biol Cell)
Source: Mol Biol Cell - March 30, 2024 Category: Molecular Biology Authors: Ainaz Mihanfar Faezeh Asghari Maryam Majidinia Source Type: research
Cellular and molecular mechanisms of cell damage and cell death in ischemia-reperfusion injury in organ transplantation
Mol Biol Rep. 2024 Mar 29;51(1):473. doi: 10.1007/s11033-024-09261-7.ABSTRACTIschemia-reperfusion injury (IRI) is a critical pathological condition in which cell death plays a major contributory role, and negatively impacts post-transplant outcomes. At the cellular level, hypoxia due to ischemia disturbs cellular metabolism and decreases cellular bioenergetics through dysfunction of mitochondrial electron transport chain, causing a switch from cellular respiration to anaerobic metabolism, and subsequent cascades of events that lead to increased intracellular concentrations of Na+, H+ and Ca2+ and consequently cellular edem...
Source: Mol Biol Cell - March 30, 2024 Category: Molecular Biology Authors: George J Dugbartey Source Type: research
