Measuring Connexin Hemichannel Opening in Response to an InsP3-Mediated Cytosolic Ca < sup > 2+ < /sup > Increase
Methods Mol Biol. 2024;2801:189-197. doi: 10.1007/978-1-0716-3842-2_14.ABSTRACTThe opening of connexin hemichannels (HCs) expressed at the plasma membrane of mammalian cells is regulated by a number of physiological parameters, including extracellular and intracellular Ca2+ ions. Submicromolar variations of the cytosolic Ca2+ concentration ([Ca2+]c) are per se sufficient to trigger extracellular bursts of messenger molecules through connexin HCs, thus mediating paracrine signaling. In this chapter, we present a quantitative method to measure the opening dynamics of connexin HCs expressed in a single HeLa cell upon stimulat...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Erva Bayraktar Mario Bortolozzi Source Type: research
Determination of anti-cancer effects of Nigella sativa seed oil on MCF7 breast and AGS gastric cancer cells
CONCLUSIONS: Our findings indicate that N. sativa oil has a dose-dependent effect on cytotoxicity and the expression of apoptotic, heat shock proteins, and matrix metalloproteinases genes in breast and gastric cancer.PMID:38578469 | DOI:10.1007/s11033-024-09453-1 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: İrfan Çınar Bet ül Gıdık Ebubekir Dirican Source Type: research
Harnessing potential of avian eggshell membrane derived collagen hydrolysate for bone tissue regeneration
CONCLUSIONS: The maximum ratio of Col-h/PVA that produced well defined hydrogels was 50:50. Though all the hydrogel matrices alluded towards their competitive attributes and applicability towards restorative bone repair, the hydrogel with 40:60 ratios showed better mechanical strength and cell proliferation than its counterparts. The prominent E. coli growth inhibition by the hydrogel matrices was also observed, along with excellent biocompatibility with MG-63 osteoblasts. The findings indicate strongly the promising application of avian eggshell-derived Col-h in supporting bone regeneration.PMID:38578512 | DOI:10.1007/s11...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Aakriti Aggarwal Debasish Nath Asish Pal Mahesh Kumar Sah Source Type: research
Characterization of Bitter Taste Receptor-Dependent Autophagy in Oral Epithelial Cells
Methods Mol Biol. 2024 Apr 6. doi: 10.1007/7651_2024_531. Online ahead of print.ABSTRACTMicrobial dysbiosis is an important trigger in the development of oral diseases. Oral keratinocytes or gingival epithelial cells (GECs) offer protection against various microbial insults. Recent studies suggest that GECs expressed higher level of bitter taste receptor 14 (T2R14) compared to other taste receptors and toll-like receptors and act as innate immune sentinels. Macroautophagy or autophagy is a cellular conserved process involved in the regulation of host innate immune responses against microbial infection. Here, we describe a ...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Nisha Singh Saeid Ghavami Prashen Chelikani Source Type: research
Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived Fibrin Microbeads
Methods Mol Biol. 2024 Apr 6. doi: 10.1007/7651_2024_533. Online ahead of print.ABSTRACTVolumetric muscle loss (VML) is one of the major types of soft tissue injury frequently encountered worldwide. In case of VML, the endogenous regenerative capacity of the skeletal muscle tissue is usually not sufficient for complete healing of the damaged area resulting in permanent functional musculoskeletal impairment. Therefore, the development of new tissue engineering approaches that will enable functional skeletal muscle regeneration by overcoming the limitations of current clinical treatments for VML injuries has become a critica...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Şükran Şeker Özge Lalegül-Ülker Ay şe Eser Elçin Ya şar Murat Elçin Source Type: research
Indomethacin restrains cytoplasmic nucleic acid-stimulated immune responses by inhibiting the nuclear translocation of IRF3
J Mol Cell Biol. 2024 Apr 5:mjae015. doi: 10.1093/jmcb/mjae015. Online ahead of print.ABSTRACTThe recognition of cytosolic nucleic acid triggers the DNA/RNA sensor-IRF3 axis-mediated production of type I interferons (IFNs), which are essential for antiviral immune responses. However, the inappropriate activation of these signaling pathways is implicated in autoimmune conditions. Here, we report that indomethacin, a widely used nonsteroidal anti-inflammatory drug, inhibits nucleic acid-triggered IFN production. We found that both DNA- and RNA-stimulated IFN expression can be effectively blocked by indomethacin. Interestingl...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Miao Wang Xiao-Wei Li Sen-Chao Yuan Jie Pan Zeng-Lin Guo Li-Ming Sun Shao-Zhen Jiang Ming Zhao Wen Xue Hong Cai Lin Gu Dan Luo Ling Chen Xue-Qing Zhou Qiu-Ying Han Jin Li Tao Zhou Tian Xia Tao Li Source Type: research
Cell Crosstalk in Alveolar Microenvironment: From Lung Injury to Fibrosis
Am J Respir Cell Mol Biol. 2024 Apr 5. doi: 10.1165/rcmb.2023-0426TR. Online ahead of print.ABSTRACTAlveoli are complex microenvironments composed of various cell types, including epithelial, fibroblast, endothelial, and immune cells, which work together to maintain a delicate balance in the lung environment, ensuring proper growth, development, and an effective response to lung injuries. However, prolonged inflammation or aging can disrupt normal interactions between these cells, leading to impaired repair processes and a substantial decline in lung function. Therefore, it is essential to understand the key mechanisms und...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Licheng Song Kuan Li Huaiyong Chen Lixin Xie Source Type: research
Seeing transcription in real time
Nat Rev Mol Cell Biol. 2024 Apr 5. doi: 10.1038/s41580-024-00731-1. Online ahead of print.NO ABSTRACTPMID:38580738 | DOI:10.1038/s41580-024-00731-1 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Tineke L Lenstra Source Type: research
Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability
This study offers a network perspective on p53 stability regulation, potentially guiding strategies to reinforce wild-type p53 or target mutant p53 in cancer.PMID:38580884 | DOI:10.1038/s44320-024-00032-x (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: YiQing L ü Tiffany Cho Saptaparna Mukherjee Carmen Florencia Suarez Nicolas S Gonzalez-Foutel Ahmad Malik Sebastien Martinez Dzana Dervovic Robin Hyunseo Oh Ellen Langille Khalid N Al-Zahrani Lisa Hoeg Zhen Yuan Lin Ricky Tsai Geraldine Mbamalu Varda Rot Source Type: research
LTBP2 regulates cisplatin resistance in GC cells via activation of the NF- κB2/BCL3 pathway
In conclusion, silencing LTBP2 could effectively inhibit cell proliferation and mitigate cisplatin resistance via the NFKB noncanonical pathway NFKB2 p52/Bcl-3/cyclin D1. These findings propose LTBP2 as a potential therapeutic target for overcoming cisplatin resistance in GC patients.PMID:38577985 | DOI:10.1590/1678-4685-GMB-2023-0231 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Jun Wang Wenjia Liang Xiangwen Wang Zhao Chen Lei Jiang Source Type: research
Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells
CONCLUSIONS: Collectively, short-term regulation of TSFM level in cultured cells does not cause a significant change in proteins involved in APP processing, levels in ROS and ATP associated with mitochondrial function. Whereas our study could contribute to comprehend certain clinical features of TSFM mutations, the roles of TSFM in cardiomyopathy and cancer development might deserve further investigation.PMID:38578353 | DOI:10.1007/s11033-024-09426-4 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Xiao-Yun Li Gui-Feng Zhou Xiong-Yong Xie Ya-Lan Pu Xue -Chen Chen-Lu Li Jie Yang Lu Wang Guo-Jun Chen Source Type: research
Bovine lactoferrin inhibits inflammatory response and apoptosis in lipopolysaccharide-induced acute lung injury by targeting the PPAR- γ pathway
CONCLUSION: This study has suggested that the PPAR-γ pathway is the critical target of bLF in anti-inflammatory reactions and apoptosis of ALI, which provides a direction for further research.PMID:38578368 | DOI:10.1007/s11033-024-09436-2 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Yantao Li Junhu Li Yan Dong Can Wang Zhigang Cai Source Type: research
Anti-cancer effects of alpha lipoic acid, cisplatin and paclitaxel combination in the OVCAR-3 ovarian adenocarcinoma cell line
CONCLUSIONS: This study is the first one to investigate the combined treatment of ALA, Cisplatin, Paclitaxel on OVCAR-3. While ALA alone was not effective, combined therapy with ALA, has been found to reduce cell invasion, especially wound healing in the first 24 h, along with tumor cell adhesion.PMID:38578399 | DOI:10.1007/s11033-024-09422-8 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Hatice Şiyzen Çoban Nazl ı Çil Elif Önder G ülçin Abban Mete Source Type: research
Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels
Methods Mol Biol. 2024;2801:1-16. doi: 10.1007/978-1-0716-3842-2_1.ABSTRACTConnexins are the proteins that form the gap junction channels that are essential for cell-to-cell communication. These channels are formed by head-to-head docking of hemichannels (each from one of two adjacent cells). Free "undocked" hemichannels at the plasma membrane are mostly closed, although they are still important under physiological conditions. However, abnormal and sustained increase in hemichannel activity due to connexin mutations or acquired conditions can produce or contribute to cell damage. For example, mutations of Cx26, a connexin ...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Mariana C Fiori Guillermo A Altenberg Source Type: research
Expression and Function of Connexins in Extracellular Vesicles
Methods Mol Biol. 2024;2801:17-28. doi: 10.1007/978-1-0716-3842-2_2.ABSTRACTExtracellular vesicles (EVs) are recognized as major vehicles for exchange of information across distant cells and tissues, which have been extensively explored for diagnosis and therapeutic purposes. The presence of multiple connexin (Cx) proteins has been described in EVs, where they might facilitate EV-cell communication. However, quantitative changes in Cx levels and functional assessment of Cx channels have only been established for Cx43. In present work, we provide a detailed description of the protocols we have optimized to assess the expres...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: T ânia Martins-Marques Henrique Gir ão Source Type: research
