Query: siRNA

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Total 15539 results found since Jan 2013.

Semaphorin 3F Promotes Transendothelial Migration of Leukocytes in the Inflammatory Response After Survived Cardiac Arrest
AbstractLeukocyte transmigration through the blood vessel wall is a fundamental step of the inflammatory response and requires expression of adhesion molecule PECAM-1. Accumulating evidence implicates that semaphorin (Sema) 3F and its receptor neuropilin (NRP) 2 are central regulators in vascular biology. Herein, we assess the role of Sema3F in leukocyte migrationin vitro andin vivo. To determine the impact of Sema3F on leukocyte recruitmentin vivo, we used the thioglycollate-induced peritonitis model. After the induction of peritonitis, C57BL/6 mice were intraperitoneally (i.p.) injected daily with recombinant Sema3F or s...
Source: Inflammation - March 15, 2019 Category: Allergy & Immunology Source Type: research

Herpes Virus Entry Mediator (HVEM) Expression Promotes Inflammation/ Organ Injury in Response to Experimental Indirect-Acute Lung Injury
Therapeutic interventions to treat acute lung injury (ALI) remain largely limited to lung-protective strategies, as a real molecular pathophysiologically driven therapeutic intervention has yet to become available. While we have previously documented the expression of herpes virus entry mediator (HVEM) on leukocytes of septic mice and critically ill patients, its functional role in shock/sepsis-induced ALI has not yet been studied. Inasmuch, a murine model of indirect ALI (iALI) was induced by hemorrhagic shock (HEM) followed by cecal ligation and puncture (CLP), septic challenge and HVEM-siRNA or phosphate buffered saline...
Source: Shock - March 14, 2019 Category: Emergency Medicine Tags: Basic Science Aspects Source Type: research

Endoplasmic Reticulum Stress-Induced NLRP1 Inflammasome Activation Contributes to Myocardial Ischemia/Reperfusion Injury
In conclusion, NLRP1 inflammasome activation promotes myocardial I/R injury. ER stress can activate NLRP1 inflammasome via activating the NF-κB signaling pathway.
Source: Shock - March 14, 2019 Category: Emergency Medicine Tags: Basic Science Aspects Source Type: research

Safflower Yellow B Protects Brain against Cerebral Ischemia Reperfusion Injury through AMPK/NF-kB Pathway.
In conclusion, SYB protected brain I/R injury through reducing expression of inflammatory cytokines and this effect might be partly due to the inhibition of NF-κB mediated by AMPK. PMID: 30854014 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - March 14, 2019 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Parabrachial Interleukin-6 Reduces Body Weight and Food Intake and Increases Thermogenesis to Regulate Energy Metabolism
Publication date: 12 March 2019Source: Cell Reports, Volume 26, Issue 11Author(s): Devesh Mishra, Jennifer E. Richard, Ivana Maric, Begona Porteiro, Martin Häring, Sander Kooijman, Saliha Musovic, Kim Eerola, Lorena López-Ferreras, Eduard Peris, Katarzyna Grycel, Olesya T. Shevchouk, Peter Micallef, Charlotta S. Olofsson, Ingrid Wernstedt Asterholm, Harvey J. Grill, Ruben Nogueiras, Karolina P. SkibickaSummaryChronic low-grade inflammation and increased serum levels of the cytokine IL-6 accompany obesity. For brain-produced IL-6, the mechanisms by which it controls energy balance and its role in obesity remain unclear. H...
Source: Cell Reports - March 14, 2019 Category: Cytology Source Type: research

Cisplatin resistance involves a metabolic reprogramming through ROS and PGC-1 α in NSCLC which can be overcome by OXPHOS inhibition.
CONCLUSION: These results describe a new cisplatin resistance mechanism in NSCLC based on a metabolic reprogramming that is therapeutically exploitable through PGC-1α downregulation or OXPHOS inhibitors. PMID: 30880247 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - March 14, 2019 Category: Biology Authors: Cruz-Bermúdez A, Laza-Briviesca R, Vicente-Blanco RJ, García-Grande A, Coronado MJ, Laine-Menéndez S, Palacios-Zambrano S, Moreno-Villa MR, Ruiz-Valdepeñas AM, Lendinez C, Romero A, Franco F, Calvo V, Alfaro C, Acosta PM, Salas C, Garcia JM, Provencio Tags: Free Radic Biol Med Source Type: research

Cafestol Activates Nuclear Factor Erythroid-2 Related Factor 2 and Inhibits Urotensin II-Induced Cardiomyocyte Hypertrophy.
Abstract Through population-based studies, associations have been found between coffee drinking and numerous health benefits, including a reduced risk of cardiovascular disease. Active ingredients in coffee have therefore received considerable attention from researchers. A wide variety of effects have been attributed to cafestol, one of the major compounds in coffee beans. Because cardiac hypertrophy is an independent risk factor for cardiovascular events, this study examined whether cafestol inhibits urotensin II (U-II)-induced cardiomyocyte hypertrophy. Neonatal rat cardiomyocytes were exposed only to U-II (1 nM...
Source: The American Journal of Chinese Medicine - March 14, 2019 Category: Complementary Medicine Authors: Hao WR, Sung LC, Chen CC, Hong HJ, Liu JC, Chen JJ Tags: Am J Chin Med Source Type: research

Schlafen 12 mediates the effects of butyrate and repetitive mechanical deformation on intestinal epithelial differentiation in human Caco-2 intestinal epithelial cells
AbstractIntestinal epithelial differentiation may be stimulated by diverse pathways including luminal short-chain fatty acids and repetitive mechanical deformation engendered by villous motility and peristalsis. Schlafen 12 (SLFN12) is a cytosolic protein that stimulates sucrase-isomaltase (SI) expression. We hypothesized that two disparate differentiating stimuli, butyrate and repetitive deformation, would each stimulate SLFN12 expression in human Caco-2 intestinal epithelial cells and that increased SLFN12 expression would contribute to the differentiating activity of the human Caco-2 intestinal epithelial cells. We stim...
Source: Human Cell - March 14, 2019 Category: Cytology Source Type: research

Effect of PTEN loss on metabolic reprogramming in prostate cancer cells.
Authors: Zhou X, Yang X, Sun X, Xu X, Li X, Guo Y, Wang J, Li X, Yao L, Wang H, Shen L Abstract The tumor suppressor gene PTEN is one of the most often deleted genes in human prostate cancer. Loss of PTEN is an important event in prostate carcinogenesis. Metabolic reprogramming induced by PTEN loss fuels malignant growth and proliferation of prostate cancer cells. Targeted metabolomics analysis was used to investigate the effects of PTEN loss on intracellular metabolic pathways in prostate cancer cells. DU-145 cells were transfected with PTEN siRNAs (siRNA-1 and siRNA-2) for 48 h, and endogenous PTEN expression was...
Source: Oncology Letters - March 13, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Yap-Hippo promotes A549 lung cancer cell death via modulating MIEF1-related mitochondrial stress and activating JNK pathway
Publication date: May 2019Source: Biomedicine & Pharmacotherapy, Volume 113Author(s): Jiayu Zhou, Shizhen Zhang, Zhijun Li, Zhoumiao Chen, Yong Xu, Weiwen Ye, Zhengfu HeAbstractAlthough the role of Yes-associated protein (Yap) has been described in the progression of lung cancer, the downstream effector of the Yap-Hippo pathway has not been identified. Accordingly, the aim of our study is to explore whether Yap modulates the activity of lung cancer by controlling mitochondrial elongation factor 1 (MIEF1)-related mitochondrial stress in a manner dependent on the JNK pathway. Cell viability was determined via MTT, LDH releas...
Source: Biomedicine and Pharmacotherapy - March 13, 2019 Category: Drugs & Pharmacology Source Type: research

Reversine induces cell cycle arrest and apoptosis via upregulation of the Fas and DR5 signaling pathways in human colorectal cancer cells.
In conclusion, Reversine treatment suppressed tumor progression by the inhibition of cell proliferation, induction of cell cycle arrest and induction of apoptosis via upregulation of the Fas and DR5 signaling pathways in human colorectal cancer cells. The present study indicated that reversine may be used as a novel anticancer agent in human colorectal cancer. PMID: 30864676 [PubMed - as supplied by publisher]
Source: International Journal of Oncology - March 13, 2019 Category: Cancer & Oncology Authors: Park YL, Ha SY, Park SY, Choi JH, Jung MW, Myung DS, Kim HS, Joo YE Tags: Int J Oncol Source Type: research

MiR-451 antagonist protects against cardiac fibrosis in streptozotocin-induced diabetic mouse heart
Publication date: Available online 11 March 2019Source: Life SciencesAuthor(s): Cui Liang, Lu Gao, Yuan Liu, Yuzhou Liu, Rui Yao, Yapeng Li, Lili Xiao, Leiming Wu, Binbin Du, Zhen Huang, Yanzhou ZhangAbstractAimsMicroRNAs (miRNAs or miRs) are a large class of small noncoding RNAs. The present study aims to evaluate the effect of miR-451 on cardiac remodeling in diabetic cardiomyopathy.Main methodsMice were injected with streptozotocin (STZ) to induce diabetes. Twelve weeks after final STZ injection, mice were subjected to myocardial injection of adenovirus (Ad)-shmiR-451 to knock down miR-451. Mouse heart endothelial cells...
Source: Life Sciences - March 12, 2019 Category: Biology Source Type: research

PAK kinase inhibition has therapeutic activity in novel preclinical models of Adult T-cell Leukemia/Lymphoma.
CONCLUSIONS: PAK2, a gene frequently amplified in ATLL, facilitates CADM1-mediated stromal interaction and promotes survival of ATLL cells. Taken together, PAK inhibition may hold significant promise as a targeted therapy for aggressive ATLLs. PMID: 30862694 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - March 12, 2019 Category: Cancer & Oncology Authors: Chung EYL, Mai Y, Shah UA, Wei Y, Ishida E, Kataoka K, Ren X, Pradhan K, Bartholdy B, Wei X, Zou Y, Zhang J, Ogawa S, Steidl U, Zang X, Verma A, Janakiram M, Ye BH Tags: Clin Cancer Res Source Type: research

Yap-Hippo promotes A549 lung cancer cell death via modulating MIEF1-related mitochondrial stress and activating JNK pathway.
Abstract Although the role of Yes-associated protein (Yap) has been described in the progression of lung cancer, the downstream effector of the Yap-Hippo pathway has not been identified. Accordingly, the aim of our study is to explore whether Yap modulates the activity of lung cancer by controlling mitochondrial elongation factor 1 (MIEF1)-related mitochondrial stress in a manner dependent on the JNK pathway. Cell viability was determined via MTT, LDH release and immunofluorescence assays. ATP production, the mitochondrial membrane potential, and caspase-9 activity were investigated to assess mitochondrial functio...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 12, 2019 Category: Drugs & Pharmacology Authors: Zhou J, Zhang S, Li Z, Chen Z, Xu Y, Ye W, He Z Tags: Biomed Pharmacother Source Type: research

Involvement of cutaneous SR-B1 in skin lipid homeostasis.
CONCLUSION: We conclude that one of the main functions of SR-B1 in the skin is to regulate ceramide levels and thereby maintain the barrier function of the skin, resulting in the protection of cutaneous tissues from outdoor insults. PMID: 30876920 [PubMed - as supplied by publisher]
Source: Archives of Biochemistry and Biophysics - March 12, 2019 Category: Biochemistry Authors: Maria MX, Marie-Sophie N, Woodby B, Ferrara F, Florian G, Giuseppe V Tags: Arch Biochem Biophys Source Type: research

Vitexin protects against ischemia/reperfusion-induced brain endothelial permeability.
In this study, the effects of Vitexin on endothelial permeability and the underlying mechanisms in human brain microvascular endothelial cells (HBMEc) I/R injury model were investigated. Cell viability, lactate dehydrogenase (LDH), inflammation and apoptosis were detected. The effects of Vitexin on BBB integrity tight junction, matrix metalloproteinases (MMP) were also investigated. The mechanism was confirmed by PI3K inhibitor and NOS inhibitor in normal or eNOS siRNA transfection HBMEc. Vitexin significantly reduced LDH, Caspase 3 level, alleviated inflammation, also could maintain BBB integrity, increased tight junction...
Source: European Journal of Pharmacology - March 12, 2019 Category: Drugs & Pharmacology Authors: Cui YH, Zhang XQ, Wang ND, Zheng MD, Yan J Tags: Eur J Pharmacol Source Type: research

c ‑Jun/Ap‑1 is upregulated in an Ang II‑induced abdominal aortic aneurysm formation model and mediates Chop expression in mouse aortic smooth muscle cells.
c‑Jun/Ap‑1 is upregulated in an Ang II‑induced abdominal aortic aneurysm formation model and mediates Chop expression in mouse aortic smooth muscle cells. Mol Med Rep. 2019 Mar 12;: Authors: Sun D, Zhang M, Li Y, Mei S, Qin J, Yan J Abstract Abdominal aortic aneurysm (AAA) is an asymptomatic, potentially lethal disease whose ruptures have a high mortality rate. An effective pharmacological approach to decrease expansion or prevent the rupture of AAAs in humans remains lacking. Previous studies have suggested that activator protein 1 (c‑Jun/AP‑1) and C/EBP homologous protein (Chop) are invol...
Source: Molecular Medicine - March 12, 2019 Category: Molecular Biology Authors: Sun D, Zhang M, Li Y, Mei S, Qin J, Yan J Tags: Mol Med Rep Source Type: research

GSE106774 Risk SNPs mediated promoter-enhancer switching promotes prostate cancer progression through lncRNA PCAT19 (RNA-seq data sets)
Contributors : Junjie Hua ; Housheng H HeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo determine the functional mechanisms of PCAT19-long isoform and HNRNPAB, we conducted siRNA knockdown RNA-sequencing in prostate cancer cell line V16A.
Source: GEO: Gene Expression Omnibus - March 11, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

YAP1 Is Involved in Tumorigenic Properties of Prostate Cancer Cells.
In this study, YAP1 expression was upregulated in the whole cell lysates and cytoplasmic/nuclear extracts of AR negative (PC3) compared to AR positive (LNCaP) prostate cancer cell lines and primary prostate epithelial cells (PrePEC). pYAP1 expression elevated in LNCaP compared to PC3 and PrePEC in whole cell lysates and cytoplasmic extracts. The treatment of LNCaP and PC3 with YAP1-targeting siRNA oligonucleotides (YAP1 siRNA) significantly reduced their proliferation in vitro. Furthermore, treatment with YAP1 siRNA diminished the clonogenicity, anchorage-independent growth on soft agar, migration and invasion of PC3 cells...
Source: Pathology Oncology Research - March 11, 2019 Category: Pathology Authors: Collak FK, Demir U, Sagir F Tags: Pathol Oncol Res Source Type: research

Mammalian Hippo kinase pathway is downregulated by BCL-2 via protein degradation.
Abstract Mammalian ste20-like kinase (MST) signaling pathway plays a significant part in control of cell death and cell cycle. It was originally found as Hippo pathway in Drosophila and composed of MST kinase and Salvador-1 (SAV1), a scaffold protein. In mammalian cells, MST pathway induces cell-cycle exit and apoptosis in response to various signals. BCL-2, an anti-apoptotic protein, inhibits cell death and plays an important part in tumorigenesis. In the present report, we present evidence showing that BCL-2 is a new regulator of MST pathway. First, protein levels of MST2 and SAV1 were reduced significantly by c...
Source: Biochemical and Biophysical Research communications - March 11, 2019 Category: Biochemistry Authors: Won GW, Park SH, Park J, Lee Y, Lee YH Tags: Biochem Biophys Res Commun Source Type: research

Dimethylaminomicheliolide ameliorates peritoneal fibrosis through the activation of autophagy
This study aimed to investigate the protective effect of DMAMCL against PD-related PF and the mechanisms involved. In this study, we found that DMAMCL significantly decreased PD-induced extracellular matrix (ECM) deposition in a mouse model of PD, and that delayed DMAMCL administration halted the progression of PF in an established PD model. In addition, rapamycin administration induced autophagy and significantly ameliorated PF. The protective effect of DMAMCL against PF was weakened when co-administered with DMAMCL and 3-methyladenine. Inducing autophagy by rapamycin decreased transforming growth factor- β1–induced EC...
Source: Journal of Molecular Medicine - March 11, 2019 Category: Molecular Biology Source Type: research

Interleukin-1 Receptor Associated Kinase 1 Mediates the Maintenance of Neuropathic Pain after Chronic Constriction Injury in Rats.
Abstract Neuropathic pain (NP) has complicated pathogenesis as it mainly involves a lesion or dysfunction of the somatosensory nervous system and its clinical treatment remains challenging. Chronic constriction injury (CCI) model is a widely used neuropathic pain model and involved in mechanisms including both nerve inflammatory and injury. Cytokines and their receptors play essential roles in the occurrence and persistence of neuropathic pain, but the underlying mechanisms have not well been understood. Therefore, Interleukin-1 receptor-associated kinase 1 (IRAK1) is chosen to explore the possible mechanisms of N...
Source: Neurochemical Research - March 11, 2019 Category: Neuroscience Authors: Yin D, Chen Y, Li Y, Lu R, Wang B, Zhu S, Fan B, Xu Z Tags: Neurochem Res Source Type: research

Adeno-associated virus 9-mediated small RNA interference of TLR4 alleviates myocardial ischemia and reperfusion injury by inhibition of the NF- κB and MAPK signaling pathways in rats.
Conclusion AAV9-TLR4 siRNA has a positive effect on myocardial I/R injury by inhibiting the MAPK and NF-κB signaling pathways and can be used as a potential therapeutic method for myocardial I/R injury. PMID: 30854968 [PubMed - as supplied by publisher]
Source: Current Molecular Medicine - March 11, 2019 Category: Molecular Biology Authors: Xu T, Zhang K, Kan F, Li F, Yu B, Du W, Nie H Tags: Curr Mol Med Source Type: research

Dimethylaminomicheliolide ameliorates peritoneal fibrosis through the activation of autophagy.
This study aimed to investigate the protective effect of DMAMCL against PD-related PF and the mechanisms involved. In this study, we found that DMAMCL significantly decreased PD-induced extracellular matrix (ECM) deposition in a mouse model of PD, and that delayed DMAMCL administration halted the progression of PF in an established PD model. In addition, rapamycin administration induced autophagy and significantly ameliorated PF. The protective effect of DMAMCL against PF was weakened when co-administered with DMAMCL and 3-methyladenine. Inducing autophagy by rapamycin decreased transforming growth factor-β1-induced ECM a...
Source: Molecular Medicine - March 11, 2019 Category: Molecular Biology Authors: Li S, Peng F, Gong W, Wu J, Wang Y, Xu Z, Liu W, Li H, Yin B, Zhang Y, Chen S, Luo C, Li P, Chen Y, Huang Q, Zhou W, Long H Tags: J Mol Med (Berl) Source Type: research

Circular RNA ciRS-7 promotes the proliferation and metastasis of pancreatic cancer by regulating miR-7-mediated EGFR/STAT3 signaling pathway
ConclusionsCircular RNA ciRS-7 plays an oncogene role in PDAC, partly by targeting miR-7 and regulating the EGFR/STAT3 signaling pathway.
Source: Hepatobiliary and Pancreatic Diseases International - March 10, 2019 Category: Gastroenterology Source Type: research

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.
Authors: Mao H, Tang Z, Li H, Sun B, Tan M, Fan S, Zhu Y, Sun Y Abstract The primary cilium is a microtubule-based sensory organelle. The molecular mechanism that regulates ciliary dynamics remains elusive. Here, we report an unexpected finding that MLN4924, a small molecule inhibitor of NEDD8-activating enzyme (NAE), blocks primary ciliary formation by inhibiting synthesis/assembly and promoting disassembly. This is mainly mediated by MLN4924-induced phosphorylation of AKT1 at Ser473 under serum-starved, ciliary-promoting conditions. Indeed, pharmaceutical inhibition (by MK2206) or genetic depletion (via siRNA) of...
Source: Protein and Cell - March 10, 2019 Category: Cytology Tags: Protein Cell Source Type: research

MicroRNA-370 protects against myocardial ischemia/reperfusion injury in mice following sevoflurane anesthetic preconditioning through PLIN5-dependent PPAR signaling pathway
This study aims to investigate the effects of microRNA (miR-370) targeting perilipin-5 (PLIN5) in mice following sevoflurane anesthetic preconditioning (SAP). A mouse model of left ventricular myocardial IRI was established, followed by the evaluation of myocardial infarction size and cardiac function to determine the effects of SAP. The underlying regulatory mechanisms of miR-370 were analyzed in concert with the treatment of miR-370 mimic, miR-370 inhibitor, or siRNA against PLIN5 in cardiomyocytes isolated from mice with IRI. Also, cardiomyocyte proliferation, cell cycle distribution and apoptosis were evaluated followi...
Source: Biomedicine and Pharmacotherapy - March 9, 2019 Category: Drugs & Pharmacology Source Type: research

PAR2 inhibition enhanced the sensitivity of colorectal cancer cells to 5-FU and reduced EMT signaling.
In this study, we found that protease-activated receptor-2 (PAR2) induction in 5-Fu therapy is correlated with TGF-β-mediated EMT and apoptosis resistance. PAR2 and TGF-β were both activated in response to 5-Fu treatment in vivo and in vitro, and whereas TGF-β inhibition sensitized CRC cells to 5-Fu and suppressed cell migration, PAR2 activation eliminated the effect TGF-β inhibition. Conversely, siRNA-mediated PAR2 depletion or PAR2 inhibition with a specific inhibitor produced a similar phenotype as TGF-β signal inhibition: 5-Fu sensitization and cell-migration suppression. Moreover, the results of xenograft experim...
Source: Oncology Research - March 9, 2019 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Ghrelin exhibited antidepressant and anxiolytic effect via the p38-MAPK signaling pathway in hippocampus
Publication date: Available online 8 March 2019Source: Progress in Neuro-Psychopharmacology and Biological PsychiatryAuthor(s): Qiu-Qin Han, Hui-Jie Huang, Ya-Lin Wang, Liu Yang, Adam Pilot, Xiao-Cang Zhu, Rui Yu, Jing Wang, Xiao-Rong Chen, Qiong Liu, Bing Li, Gen-Cheng Wu, Jin YuAbstractGhrelin, a peptide derived from stomach, is an endogenous ligand for growth hormone secretagogue receptor (GHSR). So far, the exact role of ghrelin in depression and anxiety is still being debated. The p38 mitogen-activated protein kinase (p38-MAPK) is known to be activated in response to various stress stimuli. Thus, we hypothesize that g...
Source: Progress in Neuro Psychopharmacology and Biological Psychiatry - March 8, 2019 Category: Psychiatry Source Type: research

Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles.
Abstract Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanoparticles are then delivered to the media surrounding cells seeded onto tissue culture plastic; this technique is termed bolus delivery. Although bolus delivery is widely utilized to screen for efficient delivery vehicles and to study gene function in vitro, this delivery strategy may not result in efficient gene transfer for all cell types or may not iden...
Source: Mol Biol Cell - March 8, 2019 Category: Molecular Biology Authors: Pannier AK, Kozisek T, Segura T Tags: Methods Mol Biol Source Type: research

The protective effect of glutaredoxin 1/DJ-1/HSP70 signaling in renal tubular epithelial cells injury induced by ischemia
In this study, we aimed to gain insight into the role of GRX1 in HK-2 cells with oxygen glucose deprivation (OGD) injury, which served as an in vitro cell model of renal epithelial cell ischemic injury. We investigated the underlying regulation of GRX1, DJ-1, and HSP70 as well as the role of the GRX1/DJ-1/HSP70 signaling pathway in this model.Materials and methodsThe protein and mRNA expressions were measured by Western blot and qRT-PCR assays, respectively. GRX1 was overexpressed by transfection of pcDNA.3.1-GRX1 and DJ-1 was inhibited by transfection with DJ-1 siRNA. Cell apoptosis, caspase-3 activity, lactate dehydrogen...
Source: Life Sciences - March 8, 2019 Category: Biology Source Type: research

The JAK2/STAT3 signaling pathway is required for inflammation and cell death induced by cerulein in AR42J cells.
CONCLUSIONS: These findings point to a novel role for the JAK2/STAT3 signaling pathway in the progression of cerulein-induced AP. PMID: 30840302 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Autophagy in pulmonary macrophages mediates lung inflammatory injury via c-Src tyrosine kinase pathway activation during mechanical ventilation.
CONCLUSIONS: Our results suggested that autophagy caused VILI via regulating c-Src activation, which implies that c-Src may serve as a promising therapeutic target in VILI. PMID: 30840292 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

ZEB1 causes the production of hsa-microRNA-99b/let-7e/microRNA-125a cluster and promotes invasion of liver cancer cells.
CONCLUSIONS: Hsa-microRNA-99b/let-7e/microRNA-125a cluster is highly-expressed in hepatocarcinoma, and its expression can be regulated by ZEB1. In addition, the overexpression of this cluster can promote the invasion of liver cancer cells and advance liver cancer progression. PMID: 30840268 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MEG3 promotes liver cancer by activating PI3K/AKT pathway through regulating AP1G1.
CONCLUSIONS: Low expression of MEG3 could promote the proliferative and invasive abilities of hepatoma cells and accelerate cell cycle. The mechanism may be related to the inhibition of AP1G1 expression and activation of PI3K/AKT pathway. PMID: 30840267 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - March 8, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation
This report suggests asprosin as a novel therapeutic target for the treatment of type 2 diabetes through preservation of β-cell function.Graphical abstract
Source: Molecular and Cellular Endocrinology - March 8, 2019 Category: Endocrinology Source Type: research

GSE124757 Phosphatase inhibitor PPP1R11 modulates resistance of human T cells towards Treg-mediated suppression of TCR signaling
Contributors : Sunjay J Fernandes ; Rubin N Joshi ; Angelika SchmidtSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe have performed RNAseq analysis of human T cells upon PPP1R11 siRNA silencing. We have explored the role of PPP1R11 for the first time in primary human T cells and discovered that PPP1R11 is a novel negative mediator of T cell activation.
Source: GEO: Gene Expression Omnibus - March 8, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

MicroRNA-370 protects against myocardial ischemia/reperfusion injury in mice following sevoflurane anesthetic preconditioning through PLIN5-dependent PPAR signaling pathway.
This study aims to investigate the effects of microRNA (miR-370) targeting perilipin-5 (PLIN5) in mice following sevoflurane anesthetic preconditioning (SAP). A mouse model of left ventricular myocardial IRI was established, followed by the evaluation of myocardial infarction size and cardiac function to determine the effects of SAP. The underlying regulatory mechanisms of miR-370 were analyzed in concert with the treatment of miR-370 mimic, miR-370 inhibitor, or siRNA against PLIN5 in cardiomyocytes isolated from mice with IRI. Also, cardiomyocyte proliferation, cell cycle distribution and apoptosis were evaluated followi...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 8, 2019 Category: Drugs & Pharmacology Authors: Zhao YB, Zhao J, Zhang LJ, Shan RG, Sun ZZ, Wang K, Chen JQ, Mu JX Tags: Biomed Pharmacother Source Type: research

Desmocollin 3 has a tumor suppressive activity through inhibition of AKT pathway in colorectal cancer.
In this study, we performed cell-based functional analysis after DSC3 overexpression by stable transfection and knockdown by siRNA in CRC cells. It turned out that overexpression of DSC3 reduced cell proliferation, colony forming ability, induced G0/G1 cell cycle arrest and promoted apoptosis. Further pathway analysis showed that overexpression of DSC3 significantly inhibited the activity of AKT pathway and increased the expression of E-cadherin as well as p53 and p21. In contrast, siRNA-mediated knockdown of DSC3 increased cell proliferation and colony formation, activated the AKT pathway and decreased the expression of E...
Source: Experimental Cell Research - March 8, 2019 Category: Cytology Authors: Cui T, Yang L, Ma Y, Petersen I, Chen Y Tags: Exp Cell Res Source Type: research

Diesel exhaust particles elevate MUC5AC and MUC5B expression via the TLR4-mediated activation of ERK1/2, p38 MAPK, and NF- κB signaling pathways in human airway epithelial cells.
Diesel exhaust particles elevate MUC5AC and MUC5B expression via the TLR4-mediated activation of ERK1/2, p38 MAPK, and NF-κB signaling pathways in human airway epithelial cells. Biochem Biophys Res Commun. 2019 Mar 08;: Authors: Na HG, Kim YD, Choi YS, Bae CH, Song SY Abstract Exposure to diesel exhaust particles (DEPs) is known to cause serious health problems, owing to a steady increase in the number of diesel vehicles worldwide. DEPs comprise approximately 90% particle mass existing in the fine size range (≤2.5 μm) and are mainly absorbed in the respiratory tract. However, limited information...
Source: Biochemical and Biophysical Research communications - March 8, 2019 Category: Biochemistry Authors: Na HG, Kim YD, Choi YS, Bae CH, Song SY Tags: Biochem Biophys Res Commun Source Type: research

IQGAP1 mediates podocyte injury in diabetic kidney disease by regulating nephrin endocytosis.
Abstract Diabetic kidney disease (DKD) is a complication associated with diabetes and is a major public health problem in modern society. Podocyte injury is the central target of the development of DKD, and the loss or dysregulation of nephrin, a key structural and signalling molecule located in the podocyte slit diaphragm (SD), initiates potentially catastrophic downstream events within podocytes. IQGAP1, a scaffold protein containing multiple protein-binding domains that regulates endocytosis, can interact with nephrin in podocytes. It is hypothesized that IQGAP1 contributes to nephrin endocytosis and may partic...
Source: Cellular Signalling - March 8, 2019 Category: Cytology Authors: Liu Y, Su H, Ma C, Ji D, Zheng X, Wang P, Zheng S, Wang L, Wang Z, Xu D Tags: Cell Signal Source Type: research

Hypoxia-inducible factor (HIF)-prolyl hydroxylase 3 (PHD3) maintains high HIF2A mRNA levels in clear cell renal cell carcinoma Cell Biology
Most clear cell renal cell carcinomas (ccRCCs) have inactivation of the von Hippel–Lindau tumor suppressor protein (pVHL), resulting in the accumulation of hypoxia-inducible factor α-subunits (HIF-α) and their downstream targets. HIF-2α expression is particularly high in ccRCC and is associated with increased ccRCC growth and aggressiveness. In the canonical HIF signaling pathway, HIF-prolyl hydroxylase 3 (PHD3) suppresses HIF-2α protein by post-translational hydroxylation under sufficient oxygen availability. Here, using immunoblotting and immunofluorescence staining, qRT-PCR, and siRNA-mediated gene silencing, we s...
Source: Journal of Biological Chemistry - March 8, 2019 Category: Chemistry Authors: Petra Miikkulainen, Heidi Hogel, Fatemeh Seyednasrollah, Krista Rantanen, Laura L. Elo, Panu M. Jaakkola Tags: Cell Biology Source Type: research

miR-203 inhibits the invasion and EMT of gastric cancer cells by directly targeting Annexin A4.
Authors: Li J, Zhang B, Cui J, Liang Z, Liu K Abstract Many studies have shown that down-regulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is down-regulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of Annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells was suppressed by overexpre...
Source: Oncology Research - March 7, 2019 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

FGF2-FGFR1 pathway activation together with thymidylate synthase upregulation is induced in pemetrexed-resistant lung cancer cells.
Authors: Miura K, Oba T, Hamanaka K, Ito KI Abstract Pemetrexed (MTA) is a folate antimetabolite used for treating non-small cell lung cancer. To elucidate the mechanisms of pemetrexed resistance in lung cancer, we established pemetrexed-resistant sublines in PC9 (mutant EGFR) and H1993 (wild-type EGFR) lung adenocarcinoma cell lines (PC9-MTA, H1993-MTA). Gene expression profile comparison by microarray analyses revealed enhanced fibroblast growth factor 2 (FGF2) and FGF receptor 1 (FGFR1) expression, confirmed by Western blotting, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reacti...
Source: Oncotarget - March 7, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Tripterygium glycoside protects diabetic kidney disease mouse serum-induced podocyte injury by upregulating autophagy and downregulating β-arrestin-1.
CONCLUSIONS: This study demonstrated that tripterygium glycoside provided protection against podocyte injury induced by high-glucose serum, and that this effect was mediated by the concomitant activation of autophagy and downregulation of β-arrestin-1. PMID: 30839094 [PubMed - as supplied by publisher]
Source: Histology and Histopathology - March 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

ASAP AIE Featured Inorganic –Organic Core@Shell Nanoparticles for High-Efficiency siRNA Delivery and Real-Time Monitoring
Nano LettersDOI: 10.1021/acs.nanolett.8b04677
Source: Nano Letters - March 7, 2019 Category: Nanotechnology Authors: Xuewen He, Feng Yin, Dongyuan Wang, Ling-Hong Xiong, Ryan T. K. Kwok, Peng Fei Gao, Zheng Zhao, Jacky W. Y. Lam, Ken-Tye Yong, Zigang Li, Ben Zhong Tang Source Type: research

Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.
Abstract Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize pathogen-associated molecular patterns to initiate downstream signal cascades to active immune responses. Our previous studies reported that, in vitro human cornea epithelium cells represented a novel target of TSLP and that TSLP/TSLPR/STAT5 signaling played an important role in the response t...
Source: Experimental Eye Research - March 7, 2019 Category: Opthalmology Authors: Dai C, Wu J, Chen C, Wu X Tags: Exp Eye Res Source Type: research

Identification of rigosertib for the treatment of recessive dystrophic epidermolysis bullosa-associated squamous cell carcinoma.
CONCLUSIONS: These data support a "first in RDEB" phase II clinical trial of rigosertib to assess tumor targeting in patients with late stage, metastatic and/ or unresectable SCC. PMID: 30846478 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - March 7, 2019 Category: Cancer & Oncology Authors: Atanasova VS, Pourreyron C, Farshchian M, Lawler ME, Brown CA, Watt S, Wright S, Warkala M, Guttmann-Gruber C, Pinon-Hofbauer J, Fuentes I, Prisco M, Rashidghamat E, Has C, Salas-Alanis JC, Palisson F, Hovnanian A, McGrath JA, Mellerio J, Bauer JW, South Tags: Clin Cancer Res Source Type: research

Regulation of Proliferation and Epithelial-to-Mesenchymal Transition (EMT) of Gastric Cancer by ZEB1 via Modulating Wnt5a and Related Mechanisms.
CONCLUSIONS ZEB1 expression is increased in GC tumor tissues and is associated with pathological features. The downregulation of ZEB1 can facilitate cell apoptosis via mediating Wnt5a, further suppressing GC cell proliferation and migration, and reducing EMT occurrence. PMID: 30829316 [PubMed - in process]
Source: Medical Science Monitor - March 6, 2019 Category: Research Tags: Med Sci Monit Source Type: research

SOCS3 ablation enhances DC-derived Th17 immune response against Candida albicans by activating IL-6/STAT3 in vitro
In this study, a si-RNA method is used to repress expression of the cytokine signaling-3 suppressor (SOCS3) in murine bone marrow-DCs, and then the maturation of DCs and the subsequent T-cell response after exposure to C. albicans are monitored in vitro.Key findingsAlong with a higher expression of the DC maturation markers CD40, CD86 and MHC-II, IL-6/STAT3 is markedly upregulated in the SOCS3 siRNA-treated DCs after exposure to C. albicans as compared with control DCs. In response to DCs maturation, CD4+ T cells have an increased expression of Th17 cell markers –– including the retinoic acid-related orphan nuclear hor...
Source: Life Sciences - March 6, 2019 Category: Biology Source Type: research