Aberrant activation of Wnt/catenin signaling and overexpression of ABCG2 contributes to apoptosis down regulation and tumor progression of high grade ovarian cancer
Acta Biochim Pol. 2021 Feb 26. doi: 10.18388/abp.2020_5488. Online ahead of print.ABSTRACTSide Population (SP) cells are the small pool of CSC like progenitor cells, which are drug resistant and recapitulate tumor generation. The occurrence of SP cells is the major inference for attaining a better treatment and improved patient survival. In this work, we have isolated 6% SP cells from a high grade ovarian carcinoma. Our functional characterization of SP cells revealed that elevated ABCG2 and anti-apoptotic factors contribute to chemoresistance and increased life span of SP cells. Further, the overexpression of surface anti...
Source: Acta Biochim Pol - February 26, 2021 Category: Biochemistry Authors: Zhaohua Gui Hong Yan Jinfeng Wu Mingxun Zhang Airan Wu Jie He Source Type: research

Aberrant activation of Wnt/catenin signaling and overexpression of ABCG2 contributes to apoptosis down regulation and tumor progression of high grade ovarian cancer
Acta Biochim Pol. 2021 Feb 26. doi: 10.18388/abp.2020_5488. Online ahead of print.ABSTRACTSide Population (SP) cells are the small pool of CSC like progenitor cells, which are drug resistant and recapitulate tumor generation. The occurrence of SP cells is the major inference for attaining a better treatment and improved patient survival. In this work, we have isolated 6% SP cells from a high grade ovarian carcinoma. Our functional characterization of SP cells revealed that elevated ABCG2 and anti-apoptotic factors contribute to chemoresistance and increased life span of SP cells. Further, the overexpression of surface anti...
Source: Acta Biochim Pol - February 26, 2021 Category: Biochemistry Authors: Zhaohua Gui Hong Yan Jinfeng Wu Mingxun Zhang Airan Wu Jie He Source Type: research

MiRNA-621 exerts tumor suppressor function in gastric adenocarcinoma by targeting AURKA/GSK-3 β pathway
Acta Biochim Pol. 2021 Feb 25. doi: 10.18388/abp.2020_5452. Online ahead of print.ABSTRACTGastric adenocarcinoma is a major challenge to human health worldwide. Abnormal expression of miR-621 was found in many types of cancer. This research aimed to investigate the effects and detailed molecular mechanisms of miR-621 on gastric adenocarcinoma progression. The present study first showed that miR-621 was downregulated in gastric cancer patients, and its expression level was correlated with tumor size. MiR-621 overexpression inhibited viability, colony formation and proliferation of gastric cancer cells. AURKA was identified ...
Source: Acta Biochim Pol - February 25, 2021 Category: Biochemistry Authors: Xiao Han Hongxue Liu Xiaojun Tang Yao Zhao Source Type: research

Involvement of GPR30 in protection effect of Dexmedetomidine against myocardial ischemia/reperfusion injury in rat via AKT pathway
In conclusion, our study provides a potential target for the clinical treatment of AMI.PMID:33629579 | DOI:10.18388/abp.2020_5473 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 25, 2021 Category: Biochemistry Authors: Zheming Shao Qihong Shen Min Kong Huadong Ni Xiaomin Hou Source Type: research

MiRNA-621 exerts tumor suppressor function in gastric adenocarcinoma by targeting AURKA/GSK-3 β pathway
Acta Biochim Pol. 2021 Feb 25. doi: 10.18388/abp.2020_5452. Online ahead of print.ABSTRACTGastric adenocarcinoma is a major challenge to human health worldwide. Abnormal expression of miR-621 was found in many types of cancer. This research aimed to investigate the effects and detailed molecular mechanisms of miR-621 on gastric adenocarcinoma progression. The present study first showed that miR-621 was downregulated in gastric cancer patients, and its expression level was correlated with tumor size. MiR-621 overexpression inhibited viability, colony formation and proliferation of gastric cancer cells. AURKA was identified ...
Source: Acta Biochim Pol - February 25, 2021 Category: Biochemistry Authors: Xiao Han Hongxue Liu Xiaojun Tang Yao Zhao Source Type: research

Involvement of GPR30 in protection effect of Dexmedetomidine against myocardial ischemia/reperfusion injury in rat via AKT pathway
In conclusion, our study provides a potential target for the clinical treatment of AMI.PMID:33629579 | DOI:10.18388/abp.2020_5473 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 25, 2021 Category: Biochemistry Authors: Zheming Shao Qihong Shen Min Kong Huadong Ni Xiaomin Hou Source Type: research

miR-520h inhibits cell survival by targeting mTOR in gestational diabetes mellitus
In conclusion, miR-520h could inhibit cell viability and promote cell apoptosis by regulating mTOR expression in the GDM cell model. Hence, miR-520h might be a potential and important marker for the diagnosis and treatment of GDM.PMID:33620957 | DOI:10.18388/abp.2020_5389 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 23, 2021 Category: Biochemistry Authors: Jie Wen Xiaoxia Bai Source Type: research

miR-520h inhibits cell survival by targeting mTOR in gestational diabetes mellitus
In conclusion, miR-520h could inhibit cell viability and promote cell apoptosis by regulating mTOR expression in the GDM cell model. Hence, miR-520h might be a potential and important marker for the diagnosis and treatment of GDM.PMID:33620957 | DOI:10.18388/abp.2020_5389 (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 23, 2021 Category: Biochemistry Authors: Jie Wen Xiaoxia Bai Source Type: research

Abnormal level of paxillin in cervical cancer cells is involved in tumor progression and invasion
Acta Biochim Pol. 2021 Feb 20. doi: 10.18388/abp.2020_5379. Online ahead of print.ABSTRACTHuman papillomavirus (HPV) is the primary causative agent for the uterine cervical cancer. The expression of oncoproteins E6/E7 promotes apoptosis inhibition and increases the risk of cervical cancer progression. Some research reported that elevated expression of paxillin (PXN) stimulated cancer growth and invasion. However, the clinical significance of PXN in cervical cancer has not been well characterized so far. We found that PXN mRNA expression and protein level are significantly upregulated in cervical cancer cells compared to ad...
Source: Acta Biochim Pol - February 20, 2021 Category: Biochemistry Authors: Hongqing Gu Jing Wen Source Type: research

Abnormal level of paxillin in cervical cancer cells is involved in tumor progression and invasion.
Abstract Human papillomavirus (HPV) is the primary causative agent for the uterine cervical cancer. The expression of oncoproteins E6/E7 promotes apoptosis inhibition and increases the risk of cervical cancer progression. Some research reported that elevated expression of paxillin (PXN) stimulated cancer growth and invasion. However, the clinical significance of PXN in cervical cancer has not been well characterized so far. We found that PXN mRNA expression and protein level are significantly upregulated in cervical cancer cells compared to adjacent normal cells. Furthermore, the paxillin over-expression was corre...
Source: Acta Biochim Pol - February 20, 2021 Category: Biochemistry Authors: Gu H, Wen J Tags: Acta Biochim Pol Source Type: research

Abundance, fatty acid composition and saturation index of neutral lipids in colorectal cancer cell lines.
Abstract Lipid droplets, the dynamic organelles that store triglycerides (TG) and cholesterol esters (CE), are highly accumulated in colon cancer cells. This work studies the TG and CE subspecies profile in colon carcinoma cell lines, SW480 derived from primary tumor, and SW620 derived from a metastasis of the same tumor. It was previously reported that the total TG and CE content is dramatically higher in SW620 cells; however, TG and CE subspecies profile has not been investigated in detail. The work presented here confirms that the total TG and CE content is significantly higher in the SW620 cells. Moreover, the...
Source: Acta Biochim Pol - February 17, 2021 Category: Biochemistry Authors: Munir R, Lisec J, Jaeger C, Zaidi N Tags: Acta Biochim Pol Source Type: research

Spectroscopic characterization of the interactions of bovine serum albumin with medicinally important metal ions: platinum (IV), iridium (III) and iron (II).
Abstract Serum albumin protein plays a key role in the transportation and distribution of bioactive species including metal ions and metal-based drugs and, therefore, the nature of their binding could provide important insight for the development of new drugs. In the present investigation, binding interactions of bovine serum albumin (BSA) with three biologically important metal ions: Pt4+, Ir3+ and Fe2+ were screened using easy-to-use and cost-effective Fourier-Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. Prior to the screening, the protein and metal ions were allowed to i...
Source: Acta Biochim Pol - February 17, 2021 Category: Biochemistry Authors: Alhazmi HA, Al Bratty M, Meraya AM, Najmi A, Alam MS, Javed SA, Ahsan W Tags: Acta Biochim Pol Source Type: research

Lack of evidence of the correlation between plasma Asymmetrical Dimethylarginine correlation and IMT in type 2 diabetic patients with chronic vascular complication.
CONCLUSIONS: There was no correlation between ADMA and the maximal or mean intima-media thickness (IMT) of the common carotid artery (CCA) and internal carotid artery (ICA). We demonstrated a correlation between symmetric dimethylarginine (SDMA) concentration and CCA IMT. The results suggest that ICA IMT may serve as a marker of vascular complication among patients with diabetes. PMID: 33543609 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 5, 2021 Category: Biochemistry Authors: Fiodorenko-Dumas Z, Dumas I, Rabczynski M, Małecki R, Adamiec R, Paprocka-Borowicz M Tags: Acta Biochim Pol Source Type: research

Mediterranean, vegetarian and vegan diets as practical outtakes of EAS and ACC/AHA recommendations for lowering lipid profile.
Abstract Reduction of total cholesterol (TC) and LDL fraction (LDL-C) may be beneficial towards decreasing the risk of development of cardiovascular diseases (CVD). First and foremost, before implementing or simultaneously with pharmacological treatment, patients should be informed about lifestyle changes that may be critical to achieving a better lipid profile. Recommendations from ACC/AHA (American College of Cardiology and American Heart Association) and EAS (European Atherosclerosis Society) mainly focus on limitation of saturated fatty acids (SFA) and trans fatty acids (TFA) consumption, but additional s...
Source: Acta Biochim Pol - February 5, 2021 Category: Biochemistry Authors: Wojda A, Janczy A, Małgorzewicz S Tags: Acta Biochim Pol Source Type: research

miR-3188 Regulates proliferation and apoptosis of granulosa cells by targeting KCNA5 in the polycystic ovary syndrome.
In conclusion, miR-3188, a key miRNA enhanced in PCOS, promoted granulosa cell proliferation through down-regulation of KCNA5, providing a new therapeutic target for PCOS. PMID: 33533580 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - February 3, 2021 Category: Biochemistry Authors: Zhou S, Xia L, Chen Y, Guo W, Hu J Tags: Acta Biochim Pol Source Type: research

Structural studies of human muscle FBPase.
Abstract Muscle fructose-1,6-bisphosphatase (FBPase), which catalyzes the hydrolysis of fructose-1,6-bisphosphate (F1,6BP) to fructose-6-phosphate (F6P) and inorganic phosphate, regulates glucose homeostasis by controlling the glyconeogenic pathway. FBPase requires divalent cations, such as Mg2+, Mn2+, or Zn2+, for its catalytic activity; however, calcium ions inhibit the muscle isoform of FBPase by interrupting the movement of the catalytic loop. It has been shown that residue E69 in this loop plays a key role in the sensitivity of muscle FBPase towards calcium ions. The study presented here is based on five crys...
Source: Acta Biochim Pol - January 27, 2021 Category: Biochemistry Authors: Barciszewski J, Szpotkowski K, Wisniewski J, Kolodziejczyk R, Rakus D, Jaskolski M, Dzugaj A Tags: Acta Biochim Pol Source Type: research

Serendipitous crystallization of E. coli HPII catalase, a sequel to "the tale usually not told".
We report a novel crystal form of another such culprit, E. coli HPII catalase, which is a tetrameric protein of ~340 kDa molecular weight. HPII is likely to contaminate recombinant protein samples, co-purify, and then co-crystallize with the target proteins, especially if their masses in size exclusion chromatography are ~300-400 kDa. What makes this case more interesting but also parlous, is the fact that HPII can crystallize from very low concentrations, even well below 1 mg/mL. PMID: 33485289 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - January 24, 2021 Category: Biochemistry Authors: Grzechowiak M, Sekula B, Jaskolski M, Ruszkowski M Tags: Acta Biochim Pol Source Type: research

Development of a novel intraarticular injection of diclofenac for the treatment of arthritis: a preclinical study in the rabbit model.
CONCLUSION: The developed intraarticular injection was found to be within the physically and chemically accepted limits. Animals treated with the intra articular injection of diclofenac showed a significant reduction in swelling as compares to the other groups. PMID: 33480230 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - January 22, 2021 Category: Biochemistry Authors: Wen X, Huang X, Wu H Tags: Acta Biochim Pol Source Type: research

Celebrating the 75th birthday of Professor Wladek Minor, one of the most accomplished Polish-American structural biologists.
Abstract This paper describes the scientific career and accomplishments of Professor Wladek Minor, who holds an endowed chair at the University of Virginia in Charlottesville, USA. Prof. Minor is a coauthor of data processing software used by macromolecular crystallographers world-wide, as well as of structural biology servers and of a repository for raw diffraction data. He made major contributions to the validation of biostructural data, with special focus on drug design targets and reproducibility in biomedical research. He is among the most highly cited molecular biologists ever. PMID: 33476109 [PubMed - ...
Source: Acta Biochim Pol - January 21, 2021 Category: Biochemistry Authors: Jaskolski M, Wlodawer A, Dauter Z, Shabalin I, Chruszcz M Tags: Acta Biochim Pol Source Type: research

The role of glucosylceramide and glucosylceramide synthase in liver disease: from bench to bedside - Review.
Abstract The cell membrane, which is lipid-rich, is not only a simple mechanical barrier but also an important and complex component of the cell. It also communicates with the external environment. Sphingomyelin is an important class of phospholipids in the membrane that performs many functions. Interest in sphingomyelin-based liposomes, which are a critical component of cell membranes, have become the focus of intense study in recent years. Through additional research, the function of sphingomyelin and its derivatives in diseases can be gradually elucidated. Sphingomyelin consists of ceramide and its derivatives ...
Source: Acta Biochim Pol - December 27, 2020 Category: Biochemistry Authors: Gan J, Zheng SJ, Mao XR, Li JF Tags: Acta Biochim Pol Source Type: research

In-vitro activity of lipoic acid against Ureaplasma urealyticum and Ureaplasma parvum isolated from women with infections of the urogenital tract. A pilot study.
Abstract Several species of Ureaplasma bacteria are known to be present in the urogenital tract of humans, in both healthy individuals and symptomatic patients. These pathogens are associated with urogenital tract infections, infertility problems and spontaneous abortion in humans. The present study involved 77 strains of Ureaplasma species (Ureaplasma spp.), including 21 Ureaplasma urealyticum (U. urealyticum) strains and 56 Ureaplasma parvum (U. parvum) strains. Lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) are synthesized in all prokaryotic and eukaryotic cells. Research of recent years increa...
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Biernat-Sudolska M, Rojek-Zakrzewska D, Bilska-Wilkosz A Tags: Acta Biochim Pol Source Type: research

Over expression of PTEN induces apoptosis and prevents cell proliferation in breast cancer cells.
Abstract The phosphatase and tensin homolog (PTEN) is a tumor suppressor lipid phosphatase frequently mutated or deleted in breast cancer cells. Loss of PTEN is associated with aberrant activation of P13K/AKT signaling pathways, which are responsible for uncontrolled cell cycle, migration and prolonged survival. Therefore, stability and functional PTEN is essential for prevention of cancer growth and migration. In the present study, we have determined the effect of PTEN over expression in apoptosis induction and cell proliferation in breast cancer cells. We showed that PTEN over expression significantly declined t...
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Wu J, Gao H, Ge W, He J Tags: Acta Biochim Pol Source Type: research

Exopolysaccharides from Lactobacillus plantarum YW11 improve immune response and ameliorate inflammatory bowel disease symptoms.
This study showed that EPS might help in modulation of gut microbiota and improve the immunity of the host to reduce the risk of IBD symptoms. PMID: 33332076 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Min Z, Xiaona H, Aziz T, Jian Z, Zhennai Y Tags: Acta Biochim Pol Source Type: research

Dexmedetomidine alleviates pulmonary ischemia-reperfusion injury through modulating the miR-21-5p/Nr4a1 signaling pathway.
This study aims to investigate the protection of dexmedetomidine (Dex) against pulmonary ischemia-reperfusion injury (PIRI) in the mouse model and reveal the mechanism in hypoxia reoxygenation (H/R)-induced mouse pulmonary vascular endothelial cells (MPVECs). The lung wet-to-dry weight ratio, histopathological features, and malondialdehyde (MDA) concentrations were measured. The H/R-induced MPVECs were exposed to Dex, and the cell viability, cell apoptosis and protein expressions were assessed by the Cell Counting Kit-8 (CCK8) assay, flow cytometry and western blot, respectively. In addition, the regulatory relationship be...
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Dong W, Yang H, Cheng M, Zhang X, Yin J, Zeng Z, Huang G Tags: Acta Biochim Pol Source Type: research

The The renin-angiotensin-aldosterone system blockade and arterial stiffness in renal transplant recipients - a cross-sectional prospective observational clinical study.
Abstract INTRODUCTION: Arterial stiffness parameters can be used as a predictor of cardiovascular events in the general population and renal transplant recipients (RTRs). Additionally, the renin-angiotensin-aldosterone-system (RAAS) blockade mitigates arterial stiffness in the general population. There are no sufficient data concerning the role of the RAAS blockade in reducing arterial stiffness among patients after kidney transplantation. The aim of this study is to assess the influence of the above blockade on arterial stiffness in RTRs. METHODS: 344 stable RTRs were enrolled in the study. 204 (59.3%) of th...
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Heleniak Z, Illersperger S, Brakemeier S, Bach P, Dębska-Ślizień A, Budde K, Halleck F Tags: Acta Biochim Pol Source Type: research

miR-590-3p Alleviates diabetic peripheral neuropathic pain by targeting RAP1A and suppressing infiltration by the T cells.
CONCLUSION: miR-590-3p ameliorates DPNP via targeting RAP1A and inhibiting T cells infiltration, indicating that exogenous miR-590-3p may be a potential candidate for clinical treatment of DPNP. PMID: 33332780 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Wu Y, Gu Y, Shi B Tags: Acta Biochim Pol Source Type: research

Resveratrol administration prevents radiation-related changes in metabolic profiles of hearts 20 weeks after irradiation of mice with a single 2 Gy dose.
;ł M Abstract We aimed to evaluate whether resveratrol affects radiation-induced changes in metabolite profiles of the mouse heart. Hearts were irradiated in vivo with a single 2 Gy dose during the resveratrol administration and metabolite profiles of heart tissue were analyzed by the untargeted HR-MAS NMR approach twenty weeks after irradiation. The administration of resveratrol mitigated the radiation-induced decline in the content of choline-containing compounds and unsaturated lipids, which might reflect the stabilization of cell membrane structure against radiation-related damage. Results obtained with this ...
Source: Acta Biochim Pol - December 17, 2020 Category: Biochemistry Authors: Gramatyka M, Widłak P, Gabryś D, Kulik R, Sokół M Tags: Acta Biochim Pol Source Type: research

RAB11A mediates the proliferation and motility of esophageal cancer cells via WNT signaling pathway.
In this study, RAB11A was upregulated in human EC tissues and cells and predicted poor diagnosis. RAB11A expression was correlated with clinical-pathological features including pTNM stage (P=0.001*) and recurrence (P=0.000**) in patients with EC. Furthermore, RAB11A knockdown decreased the proliferation, migration, and invasion of EC cells via WNT pathway in vitro. Subsequently, the in vivo experiments confirmed that RAB11A contributed to EC tumor growth via WNT pathway. Therefore, these results provided evidence showing that RAB11A could promote the progression of EC via WNT pathway and might serve as a promising therapeu...
Source: Acta Biochim Pol - December 16, 2020 Category: Biochemistry Authors: Zhao D, Wang B, Chen H Tags: Acta Biochim Pol Source Type: research

Impact of diet and synbiotics on selected gut bacteria and intestinal permeability in individuals with excess body weight - A Prospective, Randomized Study.
Abstract Overweight and obese individuals may have leaky intestinal barrier and microbiome dysbiosis. The aim of this study was to determine whether body mass reduction with diet and synbiotics in an adult person with excess body mass has an influence on the gut microbiota and zonulin concentration. The study was a single blinded trial. 60 persons with excess body mass were examined. Based on randomization, patients were qualified either to the intervention group (Synbiotic group) or to the control group (Placebo group). Anthropometric measurements, microbiological assessment of faecal samples and zonulin concentr...
Source: Acta Biochim Pol - December 16, 2020 Category: Biochemistry Authors: Janczy A, Aleksandrowicz-Wrona E, Kochan Z, Małgorzewicz S Tags: Acta Biochim Pol Source Type: research

Downregulated NORAD in neuroblastoma promotes cell proliferation via chromosomal instability and predicts poor prognosis.
Abstract Increasing evidence suggests that long non-coding RNAs (lncRNAs) are involved in neuroblastoma (NB) pathogenesis. The aim of this study was to elucidate the roles and underlying mechanism of non-coding RNA activated by DNA damage (NORAD) in childhood NB. Both public data and clinical specimens were used to determine NORAD expression. Colony formation, cell proliferation and wound healing assays were performed to evaluate NORAD effects on proliferation and migration of SH-SY5Y and SK-N-BE(2) cells. Flow cytometry was used to examine the cell cycle changes. The expression of genes and proteins involved in c...
Source: Acta Biochim Pol - December 16, 2020 Category: Biochemistry Authors: Yu Y, Chen F, Jin Y, Yang Y, Wang S, Zhang J, Chen C, Zeng Q, Han W, Wang H, Guo Y, Ni X Tags: Acta Biochim Pol Source Type: research

Heat shock protein 90 α inhibitor, PU-H71 in combination with DHEA promoting apoptosis in triple-negative breast cancer cell line MDA-MB-231.
Heat shock protein 90α inhibitor, PU-H71 in combination with DHEA promoting apoptosis in triple-negative breast cancer cell line MDA-MB-231. Acta Biochim Pol. 2020 Dec 15;: Authors: Soudan H, Saeed H, Eldemellawy M, Shalaby M, Hassan M, Elkewedi M, El-Nikhely N Abstract Due to the lack of markers (ER, PR, and HER-2/Neu) for the molecular-targeted therapies triple-negative breast cancer (TNBC) is more challenging than other subtypes of breast cancer. Moreover, the conventional chemotherapeutic agents are still the mainstay of most therapeutic protocols and eventually turn into a refractory drug-r...
Source: Acta Biochim Pol - December 15, 2020 Category: Biochemistry Authors: Soudan H, Saeed H, Eldemellawy M, Shalaby M, Hassan M, Elkewedi M, El-Nikhely N Tags: Acta Biochim Pol Source Type: research

Interaction network of proteins associated with unfavorable prognosis in acute myeloid leukemia.
Abstract Acute myeloid leukemia (AML) is a malignant disorder of hematopoietic stem and progenitor cells, characterized by accumulation of immature blasts in the bone marrow and peripheral blood of affected patients. Standard induction therapy leads to complete remission in approximately 50% to 75% of patients. In spite of favorable primary response rates, only 20% to 30% of patients enjoy long-term disease free survival. Identifying proteins involved in prognosis is important for proposing biomarkers that can aid in the clinical management of the disease. The aim of this study was to construct a protein-protein i...
Source: Acta Biochim Pol - December 7, 2020 Category: Biochemistry Authors: Rendon-Rodriguez JJ, Restrepo-Rodriguez LF, Rothlisberger S Tags: Acta Biochim Pol Source Type: research

MiR-149 attenuates the proliferation and migration of TGF- β1-induced airway smooth muscle cells by targeting TRPM7 and affecting downstream MAPK signal pathway.
In this study, the expression level of miR-149 in human airway smooth muscle cells (ASMCs) was decreased after TGF-β1 treatment in vitro. Additionally, the over-expression of miR-149 obviously suppressed proliferation and migration in human ASMCs. Besides, we found that overexpression of miR-149 could inhibit the expression of transient receptor potential melastatin 7 (TRPM7) both in protein and gene levels. Furthermore, we demonstrated that miR-149 could inhibit the cell proliferation and migration in human ASMCs by targeting TRPM7 through modulating mitogen-activated protein kinases (MAPKs) signaling pathway. Taken ...
Source: Acta Biochim Pol - December 7, 2020 Category: Biochemistry Authors: Zhu Z, Zhang L, Jiang T, Qian Y, Sun Y, Zhang Q Tags: Acta Biochim Pol Source Type: research

Development of a 12-biomarkers-based prognostic model for pancreatic cancer using multi-omics integrated analysis.
Abstract Pancreatic cancer is one of the most malignant tumors of the digestive system, with insidious, rapid onset and high mortality. The 5-year survival rate is only 10%. Therefore, in-depth exploration of the potential mechanism affecting the prognosis of pancreatic cancer, and search for biomarkers that can effectively predict the prognosis of pancreatic cancer are of practical clinical importance. The mRNA sequencing data, miRNA sequencing data, methylation data and SNP data of pancreatic cancer patients available in The Cancer Genome Atlas (TCGA) were used for analysis to identify biomarkers that significan...
Source: Acta Biochim Pol - December 1, 2020 Category: Biochemistry Authors: Jia Y, Shen M, Zhou Y, Liu H Tags: Acta Biochim Pol Source Type: research

The evolving role of ribosomes in the regulation of protein synthesis.
Abstract Maintenance of the cellular homeostasis is firmly linked with protein synthesis. Therefore, it is tightly controlled at multiple levels. An advancement in quantitative techniques, mainly over the last decade, shed new light on the regulation of protein production, which pointed the ribosome as a new player. Ribosomes are macromolecular machines that synthesize polypeptide chains using mRNA as a template. The enormous complexity of ribosomes provides many possibilities of changes in their composition and consecutively in their target specificity. However, it is not clear how this specialization is enforced...
Source: Acta Biochim Pol - November 27, 2020 Category: Biochemistry Authors: Gościńska K, Topf U Tags: Acta Biochim Pol Source Type: research

C-C motif chemokine ligand 5 and C-C chemokine receptor type  5: possible diagnostic application in breast cancer patients.
In conclusion, our research indicates that examination of plasma CCL5 and CCR5 may be useful in BC diagnosis at the early stage of the disorder, especially when combined with CA 15-3. PMID: 33245226 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - November 27, 2020 Category: Biochemistry Authors: Dąbrowska E, Przylipiak A, Zajkowska M, Piskór BM, Borowik-Zaręba A, Ławicki S Tags: Acta Biochim Pol Source Type: research

Asthma-derived fibroblast to myofibroblast transition is enhanced in comparison to fibroblasts derived from non-asthmatic patients in 3D in vitro culture due to Smad2/3 signalling.
Abstract The basic hallmarks of bronchial asthma, one of the most common chronic diseases occurring in the world, are chronic inflammation, remodelling of the bronchial wall and its hyperresponsiveness to environmental stimuli. It was found out that the fibroblast to myofibroblast transition (FMT), a key phenomenon in subepithelial fibrosis of the bronchial wall, was crucial for the development of asthma. Our previous studies showed that HBFs derived from asthmatic patients cultured in vitro display some inherent features which facilitate their TGF-b-induced FMT. Although usefulness of standard '2D' cultures is in...
Source: Acta Biochim Pol - November 27, 2020 Category: Biochemistry Authors: Wnuk D, Lasota S, Paw M, Madeja Z, Michalik M Tags: Acta Biochim Pol Source Type: research

Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum.
Abstract Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid - IAA; 2,4-Dichlorophenoxyacetic acid - 2,4-D; kinetin - KIN; 6-Benzylaminopurine - BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basi...
Source: Acta Biochim Pol - November 27, 2020 Category: Biochemistry Authors: Lang J, Pankowski J, Grabarz P, Pluciński B, Jedynak P Tags: Acta Biochim Pol Source Type: research

Growth of mixed cancer cell population - in silico the size matters.
ka PM Abstract Cancer heterogeneity is still underexplored and difficult to investigate. The whole network of factors engaged in tumor growth makes clinical cases, as well as the in vivo and in vitro experiments, of limited use in terms of understanding cancer heterogeneity. Our idea was to start from scratch and focus on the simplest distinctive feature in a heterogeneous tumor, namely the cell size. To exclude any other factors, we created a rudimentary cellular automata model of mixed cancer culture with two lines of different cell sizes. We tested the model with various sets of parameters to exp...
Source: Acta Biochim Pol - November 26, 2020 Category: Biochemistry Authors: Kłóś A, Płonka PM Tags: Acta Biochim Pol Source Type: research

The levels of sICAM-1, sELAM-1, TNF α and sTNFR1 proteins in patients with colorectal adenocarcinoma in tumor and corresponding normal mucosa.
The levels of sICAM-1, sELAM-1, TNFα and sTNFR1 proteins in patients with colorectal adenocarcinoma in tumor and corresponding normal mucosa. Acta Biochim Pol. 2020 Nov 26;: Authors: Strzelczyk JK, Cuber P, Bochon B, Gajdzik K, Strzelczyk J, Krakowczyk Ł, Wiczkowski A, Owczarek AJ, Ostrowska Z Abstract Colorectal cancer is a common malign disease of the gastrointestinal tract. The cancer survival rate depends on the stage of the disease at detection time. It is well known that several molecular mechanisms are involved in cancer and some molecules might affect or modulate cancerogenesis. The aim...
Source: Acta Biochim Pol - November 26, 2020 Category: Biochemistry Authors: Strzelczyk JK, Cuber P, Bochon B, Gajdzik K, Strzelczyk J, Krakowczyk Ł, Wiczkowski A, Owczarek AJ, Ostrowska Z Tags: Acta Biochim Pol Source Type: research

Growth response and metabolic effects of growth hormone therapy in appropriate-for-gestational-age growth hormonedeficient children in relation to birth size and gestational age: A preliminary study.
In conclusion, our results suggest that AGA GH-deficient children born with higher birth size parameters and higher GA had better first-year growth response to rhGH therapy and better baseline metabolic profile, especially parameters of carbohydrate metabolism. In order to optimize the effects of rhGH therapy, higher rhGH doses should be considered in those GH-deficient children who were born with lower birth size and GA. PMID: 33242242 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - November 26, 2020 Category: Biochemistry Authors: Witkowska-Sędek E, Stelmaszczyk-Emmel A, Kucharska A, Rumińska M, Majcher A, Pyrżak B Tags: Acta Biochim Pol Source Type: research

Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors.
caron;tofilová J Abstract The aim of this study was to investigate the possibilities of modification of chronic disease risk factors with probiotic strain Lactobacillus plantarum LS/07 and prebiotic inulin in rats with western high fat diet. The Sprague-Dawley rats were divided into four groups: control group (CG group), group with high fat diet (HFD group), group receiving high fat diet in combination with Lactobacillus plantarum LS/07 (HFD+PRO group), and group receiving high fat diet in combination with oligofructose enriched inulin (HFD+PRE group). The activity of β-glucuronidase, lipid parameters,...
Source: Acta Biochim Pol - October 27, 2020 Category: Biochemistry Authors: Hijova E, Strojný L, Bertková I, Bomba A, Štofilová J Tags: Acta Biochim Pol Source Type: research

Resveratrol enhances apoptosis induced by the heterocyclic aromatic amines in p53-wt LoVo cells, but not in p53-deficient HaCaT cells.
Abstract In the present study, we investigated the influence of resveratrol on PhIP treated human colon cancer cells and compared the effect to HaCaT cells considered as normal, human keratinocytes. Our results show that resveratrol decreases DNA damage in both cell types, it increases the sensitivity of LoVo cells to apoptosis and has no effect on PhIP-treated HaCaT cells. We confirm that PhIP-induced apoptosis is p53 and caspase 3/7 dependent. Interestingly, normal cells such as HaCaT, which lack functional p53 are more resistant to PhIP treatment. PMID: 33108147 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - October 27, 2020 Category: Biochemistry Authors: Kuryłowicz K, Cierniak A, Madej E, Skalniak Ł, Wolnicka-Głubisz A Tags: Acta Biochim Pol Source Type: research

Biological activity of Nod factors.
Abstract Chemically, the Nod factors (NFs) are lipochitooligosaccharides, produced mainly by bacteria of the Rhizobium genus. They are the main signaling molecules involved in the initiation of symbiosis between rhizobia and legume plants. Nod factors affect plant tissues at very low concentrations, even as low as 10-12 mol/L. They induce root hair deformation, cortical cell division, and root nodules' formation in the host plant. At the molecular level, the cytoskeleton is reorganized and expression of genes encoding proteins called nodulins is induced in response to Nod factors in the cell. Action of Nod factors...
Source: Acta Biochim Pol - October 22, 2020 Category: Biochemistry Authors: Kidaj D, Krysa M, Susniak K, Matys J, Komaniecka I, Sroka-Bartnicka A Tags: Acta Biochim Pol Source Type: research

Cholesterol (Blood lipid) lowering potential of Rosuvastatin chitosan nanoparticles for atherosclerosis: Preclinical study in rabbit model.
Abstract Atherosclerosis is the condition of narrowing of arteries due to plaque buildup on the artery walls. Aortic valve calcification (AVC) is one of the reasons of atherosclerosis which leads to narrowing at the opening of the aortic valve which is commonly referred as Aortic valve stenosis (AS). The Rosuvastatin-chitosan (ROS-chitosan) nanoparticles were prepared using ionotropic gelation method. Nanoparticulate formulation was optimized by 3 factor, 2 level full factorial design to find the effect of independent variables on particle size and percentage encapsulation efficiency. Particle size, encapsulation ...
Source: Acta Biochim Pol - October 22, 2020 Category: Biochemistry Authors: Chen L, Wang C, Wu Y Tags: Acta Biochim Pol Source Type: research

Post hoc analysis of fecal samples from responders and non-responders to Lactobacillus reuteri DSM 17938 intervention.
Abstract We compared fecal samples from responders and non-responders to administration of Lactobacillus reuteri DSM 17938. Data for this post hoc analysis were collected from an RCT assessing the efficacy of L. reuteri for the management of acute gastroenteritis. Responders were defined as subjects with diarrhea lasting no longer than 48 h. 44 children (17 responders and 27 non-responders) were analyzed. There were no differences in clinical characteristics and gut colonization between both groups. In the responder group, there were significantly lower levels of five metabolites before beginning of the interventi...
Source: Acta Biochim Pol - September 17, 2020 Category: Biochemistry Authors: Szymanski H, Mlynarz P, Qasem B, Korzeniowska-Kowal A, Szponar B, Kałwak-Baran M, Szajewska H Tags: Acta Biochim Pol Source Type: research

Evaluation of single nucleotide polymorphisms identified through the use of SNP assay in Romanian and Chinese Holstein and Simmental cattle breeds.
Abstract Simmental and Holstein cattle, being among the most widely distributed breeds worldwide, have been subjected to continuous selection for distinct purposes. In the current study, we evaluated the levels of SNPs identified through the use of SNP assay in Romanian Holstein and Romanian Simmental cattle, which then were compared to the data from Chinese Holstein and Chinese Simmental cattle. In total, 282 animals were genotyped: Romanian Holstein (n=30), Romanian Simmental (n=22), Chinese Holstein (n=96) and Chinese Simmental cattle (n=136), using 39,724 common SNPs to analyze minor allele frequency, genetic ...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Ilie DE, Gao Y, Nicolae I, Sun D, Li J, Han B, Gavojdian D Tags: Acta Biochim Pol Source Type: research

Be cautious during the interpretation of arterial blood gas analysis performed outside the intensive care unit.
Abstract INTRODUCTION: Reliable results of an arterial blood gas (ABG) analysis are crucial for the implementation of appropriate diagnostics and therapy. We aimed to investigate the differences (Δ) between ABG parameters obtained from point-of-care testing (POCT) and central laboratory (CL) measurements, taking into account the turnaround time (TAT). MATERIALS AND METHODS: A number of 208 paired samples were collected from 54 intensive care unit (ICU) patients. Analyses were performed using Siemens RAPIDPoint 500 Blood Gas System on the samples just after blood retrieval at the ICU and after delivery t...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Krzych L, Wojnarowicz O, Ignacy P, Dorniak J Tags: Acta Biochim Pol Source Type: research

General overview on the role of Peptidoglycan Recognition Proteins in insect immunity.
Abstract The insect immune system is responsible for maintaining the homeostasis of organisms. If the pathogen is able to breach the defensive barriers of the host, cellular and humoral mechanisms are triggered. Initiation of effective defence response is possible thanks to pathogen-associated molecular patterns, among which peptidoglycan recognition proteins play a prominent role. They recognize pathogen-associated molecular patterns and some of them also have enzymatic activity. The main aim of peptidoglycan recognition proteins is to activate pathways regulating the synthesis of immune peptides. Some of the pep...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Kordaczuk J, Sułek M, Wojda I Tags: Acta Biochim Pol Source Type: research

Condensed phase properties of n-pentadecane emerging from application of biomolecular force fields.
zyn K Abstract For over 20 years, the OPLS-All Atom (OPLS-AA) force field has been efficiently used in molecular modelling studies of proteins, carbohydrates and nucleic acids. OPLS-AA is successfully applied in computer modelling of many organic compounds, including decane and shorter alkanes, but it fails when employed for longer linear alkanes, whose chemical structure corresponds to hydrocarbon tails in phospholipids constituting cellular membranes. There have been several attempts to address this problem. In this work, we compare the ability to reproduce various condensed phase properties by six distinct sets...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Bratek M, Wójcik-Augustyn A, Kania A, Majta J, Murzyn K Tags: Acta Biochim Pol Source Type: research