Post hoc analysis of fecal samples from responders and non-responders to Lactobacillus reuteri DSM 17938 intervention.
Abstract We compared fecal samples from responders and non-responders to administration of Lactobacillus reuteri DSM 17938. Data for this post hoc analysis were collected from an RCT assessing the efficacy of L. reuteri for the management of acute gastroenteritis. Responders were defined as subjects with diarrhea lasting no longer than 48 h. 44 children (17 responders and 27 non-responders) were analyzed. There were no differences in clinical characteristics and gut colonization between both groups. In the responder group, there were significantly lower levels of five metabolites before beginning of the interventi...
Source: Acta Biochim Pol - September 17, 2020 Category: Biochemistry Authors: Szymanski H, Mlynarz P, Qasem B, Korzeniowska-Kowal A, Szponar B, Kałwak-Baran M, Szajewska H Tags: Acta Biochim Pol Source Type: research

Evaluation of single nucleotide polymorphisms identified through the use of SNP assay in Romanian and Chinese Holstein and Simmental cattle breeds.
Abstract Simmental and Holstein cattle, being among the most widely distributed breeds worldwide, have been subjected to continuous selection for distinct purposes. In the current study, we evaluated the levels of SNPs identified through the use of SNP assay in Romanian Holstein and Romanian Simmental cattle, which then were compared to the data from Chinese Holstein and Chinese Simmental cattle. In total, 282 animals were genotyped: Romanian Holstein (n=30), Romanian Simmental (n=22), Chinese Holstein (n=96) and Chinese Simmental cattle (n=136), using 39,724 common SNPs to analyze minor allele frequency, genetic ...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Ilie DE, Gao Y, Nicolae I, Sun D, Li J, Han B, Gavojdian D Tags: Acta Biochim Pol Source Type: research

Be cautious during the interpretation of arterial blood gas analysis performed outside the intensive care unit.
Abstract INTRODUCTION: Reliable results of an arterial blood gas (ABG) analysis are crucial for the implementation of appropriate diagnostics and therapy. We aimed to investigate the differences (Δ) between ABG parameters obtained from point-of-care testing (POCT) and central laboratory (CL) measurements, taking into account the turnaround time (TAT). MATERIALS AND METHODS: A number of 208 paired samples were collected from 54 intensive care unit (ICU) patients. Analyses were performed using Siemens RAPIDPoint 500 Blood Gas System on the samples just after blood retrieval at the ICU and after delivery t...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Krzych L, Wojnarowicz O, Ignacy P, Dorniak J Tags: Acta Biochim Pol Source Type: research

General overview on the role of Peptidoglycan Recognition Proteins in insect immunity.
Abstract The insect immune system is responsible for maintaining the homeostasis of organisms. If the pathogen is able to breach the defensive barriers of the host, cellular and humoral mechanisms are triggered. Initiation of effective defence response is possible thanks to pathogen-associated molecular patterns, among which peptidoglycan recognition proteins play a prominent role. They recognize pathogen-associated molecular patterns and some of them also have enzymatic activity. The main aim of peptidoglycan recognition proteins is to activate pathways regulating the synthesis of immune peptides. Some of the pep...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Kordaczuk J, Sułek M, Wojda I Tags: Acta Biochim Pol Source Type: research

Condensed phase properties of n-pentadecane emerging from application of biomolecular force fields.
zyn K Abstract For over 20 years, the OPLS-All Atom (OPLS-AA) force field has been efficiently used in molecular modelling studies of proteins, carbohydrates and nucleic acids. OPLS-AA is successfully applied in computer modelling of many organic compounds, including decane and shorter alkanes, but it fails when employed for longer linear alkanes, whose chemical structure corresponds to hydrocarbon tails in phospholipids constituting cellular membranes. There have been several attempts to address this problem. In this work, we compare the ability to reproduce various condensed phase properties by six distinct sets...
Source: Acta Biochim Pol - September 16, 2020 Category: Biochemistry Authors: Bratek M, Wójcik-Augustyn A, Kania A, Majta J, Murzyn K Tags: Acta Biochim Pol Source Type: research

New mutations in KCNT2 gene causing early infantile epileptic encephalopathy type 57: Case study and literature review.
CONCLUSIONS: Our findings implicate the genotype-phenotype correlation of these mutations. Furthermore, the computational analysis showed their effect on protein binding site and function suggesting their role in the development of early infantile epileptic encephalopathy 57. PMID: 32931186 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 14, 2020 Category: Biochemistry Authors: Alagoz M, Kherad N, Bozkurt S, Yuksel A Tags: Acta Biochim Pol Source Type: research

Determination of amino acid profile for argininosuccinic aciduria disorder using High-Performance Liquid Chromatography with fluorescence detection.
Abstract Argininosuccinic aciduria is an autosomal, recessive amino acid disorder that is caused by a deficiency of the argininosuccinate lyase enzyme. Citrulline is the most significant marker to detect this disorder. We used the High-performance liquid chromatography with fluorescence detection with 450 nm emission and 330 nm excitation wavelengths, 15 mmol/L potassium dihydrogen phosphate and 5 mmol/L dipotassium hydrogen phosphate as Mobile Phase A, and 50 mL water, 250 mL acetonitrile, and 200 mL methanol as Mobile Phase B in gradient mode with flow rate of 1.2 mL/min. The citrulline concentration was 22 &mic...
Source: Acta Biochim Pol - September 13, 2020 Category: Biochemistry Authors: Salmanizadeh H, Sahi N Tags: Acta Biochim Pol Source Type: research

The endogenous ligand for guanylate cyclase-C activation reliefs intestinal inflammation in the DSS colitis model.
Abstract Ulcerative colitis (UC) is a major type of inflammatory bowel disease (IBD) and significantly impacts patient quality of life. Previous research revealed that the guanylate cyclase-C (GC-C) signaling pathway is associated with the severity of UC. We aimed to investigate the effect of the GC-C agonist, guanylin (Gn), on inflammatory injury in mice with colitis. An experimental UC model was established in Balb/c mice. Mesalamine served as a positive control. The Gn overexpression vector was administered once per day for 1 week. Intestinal permeability of the mice was measured using fluorescein isothiocyanat...
Source: Acta Biochim Pol - September 6, 2020 Category: Biochemistry Authors: Lan D, Wen Y, Dong X, Yang Q, Liu Y, Wang K, Li H, Miao Y Tags: Acta Biochim Pol Source Type: research

Influence of the reporter vector backbone on 2-deoxyglucose dependent promoter activation.
Abstract Reporter vectors are very often used to investigate mechanisms responsible for regulation of promoter activity. Since their first generation, many new variants were constructed to increase sensitivity and reduce background signal. However, these tools are still imperfect and can generate false results. We have found that depending on the backbone of the reporter vector, pGL3 or pGL2, different results are obtained for a eukaryotic promoter's activation by metabolic changes. These observations were done in the course of investigation of the MMP2 (matrix metalloproteinase-2) promoter regulation in response ...
Source: Acta Biochim Pol - September 3, 2020 Category: Biochemistry Authors: Solecka A, Wielento A, Jurczak J, Sowińska W, Wawro M, Kasza A Tags: Acta Biochim Pol Source Type: research

MiR-542-5p regulates the progression of diabetic retinopathy by targeting CARM1.
Abstract Diabetic retinopathy (DR), as the most frequent microvascular complication of diabetes mellitus (DM), causes vision loss and blindness in adults worldwide with increasing incidence. MicroRNAs (miRNAs) are involved in the regulation of DR. However, the role of miR-542-5p is still unknown. Here, we demonstrate that miR-542-5p is down-regulated in patients with DR and in high-glucose (HG)-treated retinal pigment epithelial cells. Moreover, miR-542-5p overexpression inhibits apoptosis in retinal pigment epithelial cells exposed to HG. The interaction between miR-542-5p and co-activator-associated arginine met...
Source: Acta Biochim Pol - August 31, 2020 Category: Biochemistry Authors: Guo N, Nulahou A, Bu Q, Liu M, Wang Y, Zhao Y, Yang L, Gao Y Tags: Acta Biochim Pol Source Type: research

A proinflammatory marker in chronic rhinosinusitis: serum calprotectin.
Abstract INTRODUCTION: Studies have shown that calprotectin has a strong pro-inflammatory effect. Elevated calprotectin levels in the serum can be used as a strong clinical marker indicating the presence of inflammation. OBJECTIVE: To investigate serum calprotectin levels in patients with chronic rhinosinusitis (CRS) and to determine the applicability of calprotectin as a potential molecular pro-inflammatory biomarker for CRS. METHODS: The study consisted of three groups: chronic rhinosinusitis with polyps (CRSwNP group), chronic rhinosinusitis without polyps (CRSwoNP), and healthy control. CRS patients ...
Source: Acta Biochim Pol - August 30, 2020 Category: Biochemistry Authors: Candar T, Baklaci D, Kuzucu I, Kayabasi S Tags: Acta Biochim Pol Source Type: research

Role of Bacterial Secretion Systems and Effector Proteins - Insight into the Pathogenicity Mechanisms in Aeromonas.
Abstract Gram-negative bacteria have developed several nanomachine channels known as type II, III, IV and VI secretion systems that enable export of effector proteins/toxins from the cytosol across the outer membrane to target host cells. Protein secretion systems are critical to bacterial virulence and interactions with other organisms. Aeromonas utilize various secretion machines e.g. two-step T2SS, a Sec-dependent system as well as one-step, Sec-independent T3SS and T6SS systems to transport effector proteins/toxins and virulence factors. Type III secretion system (T3SS) is considered the dominant virulence sys...
Source: Acta Biochim Pol - August 30, 2020 Category: Biochemistry Authors: Matys J, Turska-Szewczuk A, Sroka-Bartnicka A Tags: Acta Biochim Pol Source Type: research

Detection of a pheomelanin-like pigment by EPR spectroscopy in the mycelium of Plenodomus biglobosus.
Abstract Melanin occurrence in Plenodomus biglobosus was investigated using electron paramagnetic (spin) resonance (EPR, ESR) spectroscopy. The fungus was isolated from living and dead leaves of European ash (Fraxinus excelsior L.). Dark pigmentation of P. biglobosus mycelium in vitro, especially on the reverse, was observed. The black coloration intensified with the age of the culture and inspired us to check if the analyzed fungus species synthesizes melanin. Melanin contains unpaired electrons, thus, EPR spectroscopy was applied, as a specific technique, to verify its presence in P. biglobosus. The EPR spectrum...
Source: Acta Biochim Pol - August 27, 2020 Category: Biochemistry Authors: Pukalski J, Marcol N, Wolan N, Płonka PM, Ryszka P, Kowalski T, Latowski D Tags: Acta Biochim Pol Source Type: research

MiR-139-5p alleviates neural cell apoptosis induced by spinal cord injury through targeting TRAF3.
This study further evaluated the potential role of miR-139-5p in the neural cell apoptosis after SCI in rats. A rat SCI model was successfully established and miR-139-5p expression level in SCI rats was down-regulated compared to the sham group (sham operation group) determined by qRT-PCR. MiR-139-5p overexpression via administration with miR-139-5p agomir improved locomotor functional recovery, attenuated allodynia and hyperalgesia and alleviated neural cell apoptosis in SCI rats. In addition, TRAF3 (TNF receptor-associated factor 3 ) was identified to be a target of miR-139-5p by searching the proposed target genes in Ta...
Source: Acta Biochim Pol - August 26, 2020 Category: Biochemistry Authors: Zhang Z, Shen L, Yan Y Tags: Acta Biochim Pol Source Type: research

Recent developments of MALDI MSI application in plant tissues analysis.
Abstract Mass spectrometry imaging (MSI) combined with matrix-assisted laser desorption/ionization (MALDI) is an efficient technology applied in plant metabolomics research. This technique allows for visualization of spatial distribution of metabolites such as: lipids, proteins, peptides and DNA sequences, by determining the x, y coordinates of the compounds exactly in plant tissue. Simplicity of the tissue preparation without the need of prior exact knowledge about the analytes is a great advantage of this method. In this review, we provide an overview of experimental workflow including sample preparation, data a...
Source: Acta Biochim Pol - August 26, 2020 Category: Biochemistry Authors: Susniak K, Krysa M, Gieroba B, Komaniecka I, Sroka-Bartnicka A Tags: Acta Biochim Pol Source Type: research

Molecular characterization of a multidrug-resistant/pandrug-resistant nosocomial polymicrobial infection with Klebsiella pneumoniae, Providencia rettgeri, and Acinetobacter baumannii from Rural Maharashtra, India.
Abstract The emergence of resistance against commonly used antibiotics has become a serious global concern. The rapid development of antibiotic resistance exhibited by Enterobacteriaceae has caused an increasing concern regarding untreatable bacterial infections. Here, we isolated four pathogens from a geriatric female patient who was hospitalized for a month with ventilator-associated pneumonia (VAP) and fever. The organisms isolated from the tracheal aspirates and urine included Klebsiella pneumoniae, pandrug-resistant Providencia rettgeri, and Acinetobacter baumannii. Resistome analysis indicated that the bacte...
Source: Acta Biochim Pol - July 30, 2020 Category: Biochemistry Authors: Karad DD, Somani Y, Khande H, Yadav B, Kharat AS Tags: Acta Biochim Pol Source Type: research

A novel method for rapid and quantitative detection of bisphenol A in urine.
Abstract Bisphenol A (BPA) is classified as an endocrine disruptor (ED) and it can interact with variety of hormone receptors leading to hormonal disruption and increased risk of various adverse health effects. Reducing human exposure to BPA is one of the main challenges of public health, as it is constantly present in daily life. A low-cost and commonly applied method to enable determination of BPA in the patient's body has yet to be developed. Currently available techniques are expensive, time-consuming, and require access to highly equipped analytical chemistry laboratories. Here we describe a fast and cheap en...
Source: Acta Biochim Pol - July 29, 2020 Category: Biochemistry Authors: Rutkowska A, Olsson A, Namieśnik J, Milewicz A, Ludwicki JK, Struciński P, Graczyk S Tags: Acta Biochim Pol Source Type: research

Dendritic cells' characteristics in patients with treated systemic lupus erythematosus.
Abstract The systemic lupus erythematosus (SLE) is a chronic autoimmune disease related to a loss of immune tolerance against autoantigens that leads to tissue inflammation and organ dysfunction. Constant stimulation of dendritic cells (DC) with autoantigens is hypothesized to increase the B cells' activity which are involved in production of autoantibodies that play an essential role in the SLE development. We focused our study on detecting alterations in DCs at the cellular and molecular levels in patients with treated SLE, depending on the disease activity and treatment. In order to phenotype subpopulations of ...
Source: Acta Biochim Pol - July 29, 2020 Category: Biochemistry Authors: Wardowska A, Smoleńska Ż, Lisowska KA, Zdrojewski Z, Pikuła M Tags: Acta Biochim Pol Source Type: research

Ultrasound-assisted transdermal delivery of alendronate for the treatment of osteoporosis.
Abstract The aim of the current research work was to develop sonophoresis-assisted transdermal patches for the treatment of osteoporosis. In the present investigation, we formulated alendronate-chitosan nanoparticles by ionotropic external gelation method. The prepared nanoparticles were found to be smooth and free-flowing. The optimized formulation showed 82.7% of drug release over a period of 12 hours with 99.54% EE, the particle size of 250 nm, PDI 0.22 and zeta potential of 28 mV. The solvent casting evaporation method was used for the development of the patches using HPMC as rate-controlling polymer and dibut...
Source: Acta Biochim Pol - June 18, 2020 Category: Biochemistry Authors: Li B, Huang G, Ma Z, Qin S Tags: Acta Biochim Pol Source Type: research

Changes in daily life reduce indoor exposure to selected endocrine disruptors in the home environment: a pilot intervention study.
Abstract Increasing prevalence of lifestyle diseases raised global awareness about health consequences of human exposure to endocrine disruptors (EDs): synthetic chemicals that mimic natural hormones and affect the biochemical and endocrine balance. As home environment is one of the main sources of the exposure to xenobiotics - especially for pregnant women, infants and young children - health organizations emphasize the need of implementing lifestyle changes to protect human health and child development. The aim of this study was to evaluate the effectiveness of introducing changes in daily life in lowering the e...
Source: Acta Biochim Pol - June 18, 2020 Category: Biochemistry Authors: Rutkowska A, Olsson A, Piotrowska-Szypryt M, Namieśnik J Tags: Acta Biochim Pol Source Type: research

Long non-coding RNA LINC00662 promotes proliferation and migration of breast cancer cells via regulating the miR-497-5p/EglN2 axis.
Abstract Previous reports indicated that long noncoding RNA 662 (LINC00662) plays a crucial role in several human cancers. Here, we studied the expression pattern of LINC00662 and explored its function in human breast cancer. The expression level of LINC00662 was determined in human breast cancer cell lines and tissues by real-time quantitative polymerase chain reaction (RT-qPCR). Cytoplasmic and nuclear RNA from MDA-MB-157 cells were extracted to analyze the subcellular location of LINC00662. Moreover, the MTT assay, wound-healing assay, colony-forming assay and transwell assay were employed in MDA-MB-157 cells t...
Source: Acta Biochim Pol - June 18, 2020 Category: Biochemistry Authors: Xu W, Xing Z, Zhang P, Xu W Tags: Acta Biochim Pol Source Type: research

Neogambogic acid induces apoptosis of melanoma B16 cells via the PI3K/Akt/mTOR signaling pathway.
Abstract BACKGROUND: Neogambogic acid, as one of the main components of gamboge, exhibits high activities against various tumors. OBJECTIVE: To explore the mechanism by which melanoma B16 cell apoptosis was induced by neogambogic acid. METHODS: Melanoma B16 cells were treated with different concentrations of neogambogic acid solutions (0, 1.5, 3.0, 6.0 μM). The proliferation inhibition rate was measured by MTT assay. Cell morphology was observed by inverted microscope. Cell migration and invasion were tested by Transwell assay. Flow cytometry was performed to detect the apoptosis rate and cell cycle o...
Source: Acta Biochim Pol - June 18, 2020 Category: Biochemistry Authors: Wu C, He J, Sun Z, Tang D, Wen C Tags: Acta Biochim Pol Source Type: research

Cis-2 and trans-2-eisocenoic fatty acids are novel inhibitors for Mycobacterium tuberculosis Protein tyrosine phosphatase A.
Abstract Small protein tyrosine phosphatase (PtpA) of Mycobacterium tuberculosis is attributed to the development of latent tuberculosis infection, and hence bocomes an interesting target for drug development. In this communication, inhibition of PtpA by naturally occurring fatty acids cis-2 and trans-2-eicosenoic acid is investigated. Mtb PtpA was heterologously expressed in Escherichia coli, and the activity of PtpA was inhibited by cis-2 and trans-2 eicosenoic fatty acids. Both compunds showed strong inhibition of PtpA activity with IC50 at low micromolar concentration. As comparison, trans-11-eicosenoic acid o...
Source: Acta Biochim Pol - June 17, 2020 Category: Biochemistry Authors: Savalas LRT, Furqon BRN, Asnawati D, 'Ardhuha J, Sedijani P, Hadisaputra S, Ningsih BNS, Syahri J Tags: Acta Biochim Pol Source Type: research

SRPX2 promotes cell proliferation and invasion via activating FAK/SRC/ERK pathway in non-small cell lung cancer.
CONCLUSION: Taken together, our findings revealed a functional role of SRPX2 in NSCLC cell proliferation, migration and invasion. The underlying mechanism was, at least partially, the activation of FAK/SRC/ERK pathway. This study provides the molecular basis for targeting SRPX2 in potential clinical application for NSCLC. PMID: 32550700 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - June 17, 2020 Category: Biochemistry Authors: Li X, Liu J, Sun H, Zou Y, Chen J, Chen Y, Chen C, Wu X Tags: Acta Biochim Pol Source Type: research

Oxypaeoniflorin improves myocardial ischemia/reperfusion injury by activating the Sirt1/Foxo1 signaling pathway.
This study was conducted to explore the effect of OPA on MI/R injury and its potential mechanism. An in vivo MI/R injury model was established by transient coronary ligation in BALB/c mice, and an in vitro hypoxia/reoxygenation (H/R) injury model was established with rat cardiomyocyte H9c2 cells. Echocardiographic assessments demonstrated that OPA significantly reduced disruption of cardiac function and improved the indicators of ejection fraction (EF) and fractional shortening (FS). The enzyme-linked immunosorbent assay (ELISA) results suggested that OPA significantly reduced the release of myocardial infarction-related f...
Source: Acta Biochim Pol - June 17, 2020 Category: Biochemistry Authors: Wang K, Hu W Tags: Acta Biochim Pol Source Type: research

Vitamin D and disease activity in rheumatoid arthritis patients: a retrospective study in a Romanian cohort.
CONCLUSIONS: There is no significant association of serum 25(OH)D with disease severity in a Western Romanian cohort with RA. However, this result could have implications for the disease management, as patients with RA could be supplemented with vitamin D even in the absence of disease activity. PMID: 32550709 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - June 17, 2020 Category: Biochemistry Authors: Sirbu E, Buleu F, Tudor A, Dragan S Tags: Acta Biochim Pol Source Type: research

Excessive amount and activity of μ-calpain affects apoptotic machinery in chronic B-cell leukemia cells and influences the course of the disease.
In this study we attempted to estimate a potential clinical value of μ-calpain in relation to B-CLL clinical staging in patients with extremely high lymphocytosis and studied the molecular mechanisms associating calpain activity with clinical progress of the disease. We observed significant correlations between the amounts of intracellular μ-calpain and clinical staging of the disease, with RAI stage 1 corresponding to the highest calpain amounts in the leukemic cells. There was also a positive, statistically significant correlation between the amount of μ-calpain and phosphorylated (p)ZAP-70 in B-CLL lymphocytes....
Source: Acta Biochim Pol - June 15, 2020 Category: Biochemistry Authors: Łopatniuk P, Puchalska Z, Mital A, Mikosik A, Frąckowiak J, Hellmann A, Daca A, Bryl E, Fulop T, Witkowski JM Tags: Acta Biochim Pol Source Type: research

Preclinical study of Doxorubicine-loaded liposomal drug delivery for the treatment of head and neck cancer: Optimization by Box-Behnken statistical design.
Abstract The present investigation aimed at developing Doxorubicin (DOX)-loaded liposome-mediated drug delivery system for head and neck cancer. The liposomes were prepared by film hydration technique using egg phosphatidylcholine and cholesterol using Box-Behnken statistical design. The prepared liposomes were evaluated for the percentage encapsulation efficiency, particle size and in vitro release. The average particle size of the DOX-encapsulating liposomes formulated by thin-film hydration technique was between 150.5 nm and 200 nm with an average particle size of 165.80 nm. The PDI (Polydispersity index) was f...
Source: Acta Biochim Pol - June 7, 2020 Category: Biochemistry Authors: Yang B Tags: Acta Biochim Pol Source Type: research

Comparison and evaluation of three different molecular methods for detection of human Betapapillomaviruses in skin biopsies from patients with nonmelanoma skin cancer and precancerous lesions.
Abstract Betapapillomaviruses have been linked to the development of nonmelanoma skin cancers. A great diversity of these viruses in skin specimens requires the use of sensitive and reliable detection methods. There are currently no standardized assays for diagnostic purposes. A combination of several molecular methods has great practical significance and gives the opportunity to broaden the spectrum of detected Beta-HPV types. In the present study, different molecular methods for Beta-HPVs detection and genotyping were used: PCRs with different sets of primers, PCR followed by reverse hybridization and direct seq...
Source: Acta Biochim Pol - June 7, 2020 Category: Biochemistry Authors: Kopeć J, Szostek S, Sułowicz J, Zawilińska B Tags: Acta Biochim Pol Source Type: research

Coincidence of 3-methylglutaconic aciduria and duplication 5q - a case report and literature review.
We present the case of a 13-year-old boy with 3-methylglutaconic aciduria and duplication 5q. The main symptoms included myopathy, weakness, spastic paresis intensified mostly in the lower limbs, and intellectual disability. Additional studies showed elevated levels of 3-methylglutaconic acid in urine and ammonia in plasma. A duplication in region 5q23.3q31.1 was found in array-based comparative genomic hybridization. Next-generation sequencing did not reveal any pathological mutation. On the basis of the clinical picture and the results of biochemical and genetic tests 3-methylglutaconic aciduria type IV with duplication ...
Source: Acta Biochim Pol - June 7, 2020 Category: Biochemistry Authors: Zapolnik P, Sykut-Cegielska J, Pyrkosz A Tags: Acta Biochim Pol Source Type: research

Antioxidative action of polyamines in protection of phospholipid membranes exposed to ozone stress.
Abstract In the present work, Langmuir monolayers were used to study the interaction of putrescine (a cationic antioxidant) with anionic charged membranes (1,2-dioleoyl-sn-glycerol-3-phosphate) under oxidative stress caused by the presence of ozone in the water phase. Calcium ions and acidic environment were used to compare the electrostatic and antioxidant effects of putrescine with those of an inorganic cation. It has been shown that the main role of putrescine in protecting systems against oxidation is its rapid reaction with ROS. The initial rate of ROS neutralization rose as the concentration of putrescine in...
Source: Acta Biochim Pol - May 20, 2020 Category: Biochemistry Authors: Rudolphi-Szydło E, Filek M, Dyba B, Miszalski Z, Zembala M Tags: Acta Biochim Pol Source Type: research

Neonatal cholestasis due to citrin deficiency: diagnostic pitfalls.
Abstract Citrin deficiency can manifest in newborns or infants as neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD). The paper presents a case of Polish NICCD patient presenting with low birth weight, failure to thrive, prolonged cholestatic jaundice with coagulopathy and hypoalbuminemia with normal results of MS/MS newborn screening but with high blood citrulline level observed at 3 months of age. Unreported findings included N-hypoglycosylation and increased serum very-long-chain fatty acids (VLCFA), probably secondary to liver impairment. Final diagnosis was established based on whole-exome ...
Source: Acta Biochim Pol - May 20, 2020 Category: Biochemistry Authors: Lipiński P, Jurkiewicz D, Ciara E, Płoski R, Więcek S, Bogdańska A, Stradomska T, Socha P, Rokicki D, Tylki-Szymańska A, Jankowska I Tags: Acta Biochim Pol Source Type: research

Artemisinin is highly soluble in polyethylene Glycol 4000 and such solution has multiple biological effects.
In conclusion, the 12% PEG4000-assisted artemisinin solution has a good biological effect and it can be further applied in pharmaceutics, biomaterials and medicine. PMID: 32421285 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - May 17, 2020 Category: Biochemistry Authors: Dai F, Bao Y, Li Z, Chen SH, Gao LZ, Liu ZL, Cheng L, Peng Y, Tong XL Tags: Acta Biochim Pol Source Type: research

Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer.
Abstract The head and neck squamous cell carcinoma (HNSCC) is an aggressive human malignancy diagnosed in more than 800 000 new cases worldwide, and mostly arises in the oral cavity, oropharynx, larynx, hypopharynx, and nasopharynx. The study presented here aimed to determine a possible association of rs11515 and rs3088440 gene polymorphisms in the CDKN2A gene (cyclin dependent kinase inhibitor 2A), as well as rs769412 and rs937283 in the MDM2 gene (murine double minute 2), with oral cavity cancer in a sample of Polish population; CDKN2A is crucial in regulating the cell cycle while MDM2 is an oncogene invo...
Source: Acta Biochim Pol - May 6, 2020 Category: Biochemistry Authors: Gaździcka J, Gołąbek K, Strzelczyk JK, Kiczmer P, Miśkiewicz-Orczyk K, Krakowczyk Ł, Ostrowska Z, Misiołek M Tags: Acta Biochim Pol Source Type: research

Naringin induces endoplasmic reticulum stress-mediated apoptosis, inhibits β-catenin pathway and arrests cell cycle in cervical cancer cells.
This study evaluates the antiproliferative effect and the cell death mechanism induced by naringin on cervical cancer (CC) cells. Our results demonstrated that naringin exerts significant inhibition in cell viability and exhibits IC50 value 745, 764, 793 µM against C33A, SiHa, and HeLa cells respectively. Annexin V FITC and immunoblotting analysis reveal significant apoptosis induction in cells exposed to higher doses naringin. Mechanistically, naringin induces endoplasmic reticulum (ER) stress-associated cell killing in CC cells. Naringin increases the protein expression of ER stress sensors, phosphorylates eIF2&alp...
Source: Acta Biochim Pol - April 27, 2020 Category: Biochemistry Authors: Chen S, Lin R, Hu X, Shi Q, Chen H Tags: Acta Biochim Pol Source Type: research

Intracellular mechanisms of tumor cells' immunoresistance.
Abstract One of the main mechanisms for avoiding immune response by cancer cells is mediated by inducing an immunosuppressive environment in the tumor following activation of immune checkpoints, i.e. PD-1 or CTLA-4 receptor inhibitors on T lymphocytes. Interaction inhibition between PD-1 or CTLA-4 and their ligands (PD-L1, CD80, and CD85) leads to unblocking of the T-lymphocyte function, and thus destroys cancer cells. Certain intracellular signaling pathways are also involved in the development of tumor cell immunoresistance. Immunosuppressive pathways' activation blocking may increase the immunological anti-tumo...
Source: Acta Biochim Pol - April 21, 2020 Category: Biochemistry Authors: Hermanowicz J, Sieklucka B, Nosek K, Pawlak D Tags: Acta Biochim Pol Source Type: research

MiR-98-5p expression inhibits polarization of macrophages to an M2 phenotype by targeting Trib1 in inflammatory bowel disease.
In conclusion, knockdown of miR-98-5p changed macrophage polarization to the M2 phenotype by increasing Trib1 expression, thereby alleviating IBD symptoms. PMID: 32242402 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - April 2, 2020 Category: Biochemistry Authors: Peng Y, Wang Q, Yang W, Yang Q, Pei Y, Zhang W Tags: Acta Biochim Pol Source Type: research

A crystallization and preliminary X-ray diffraction study of the Arabidopsis thaliana proliferating cell nuclear antigen (PCNA2) alone and in a complex with a PIP-box peptide from Flap endonuclease 1.
Abstract DNA replication is an important event for all living organisms and the mechanism is essentially conserved from archaea, bacteria to eukaryotes. Proliferating cell nuclear antigen (PCNA) acts as the universal platform for many DNA transacting proteins. Flap endonuclease 1 (FEN1) is one such enzyme whose activity is largely affected by the interaction with PCNA. To elucidate the key interactions between plant PCNA and FEN1 and possible structural change of PCNA caused by binding of FEN1 at the atomic level, crystallization and preliminary studies of X-ray diffraction of crystals of Arabidopsis thaliana PCNA...
Source: Acta Biochim Pol - March 18, 2020 Category: Biochemistry Authors: Kowalska E, Strzałka W, Oyama T Tags: Acta Biochim Pol Source Type: research

Analytical ultracentrifugation as a tool in the studies of aggregation of the fluorescent marker, Enhanced Green Fluorescent Protein.
Abstract Enhanced green fluorescent protein (EGFP) is a fluorescent marker used in bio-imaging applications, including as an indicator of folding or aggregation of a fused partner. However, the limited maturation, low folding efficiency, and presence of non-fluorescent states of EGFP can influence the interpretation of experimental data. To measure aggregation associated with de novo folding of EGFP from a high GdnHCl concentration, the analytical ultracentrifugation method was used. Absorption detection at 280 nm allowed to monitor the presence of monomers and aggregated forms. Fluorescence detection enabled the ...
Source: Acta Biochim Pol - March 18, 2020 Category: Biochemistry Authors: Dawidziak-Pakula A, Krasowska J, Wielgus-Kutrowska B Tags: Acta Biochim Pol Source Type: research

Effect of maternal dietary supplementation with phytosterol esters on muscle development of broiler offspring.
Abstract Recently, embryo muscle development, which is crucial for postnatal skeletal muscle growth, has been investigated widely. Nutrients in ovo were suggested to be critical in embryo muscle development since the chick growth mostly relies on nutrients in eggs at the early developmental stage. Phytosterol esters (PE), which are derived from the reactions between phytosterols and fatty acids, were demonstrated to have important effects on lipid and cholesterol metabolism regulation. In order to reveal the effect of maternal lipid metabolism on the deposition of nutrients in eggs and the development of embryonic...
Source: Acta Biochim Pol - March 18, 2020 Category: Biochemistry Authors: Wang L, Zuo X, Zhao W, Zhou G, Luo L, Yang K, Shu G, Wang S, Gao P, Zhu X, Jiang Q, Wang L Tags: Acta Biochim Pol Source Type: research

The metabolic disorders associated with chronic consumption of soft and energy drinks in rats.
CONCLUSION: The high caffeine-sugar content in ED exerts a more significant influence on the metabolic pathways than SDs. Both increase the incidence of cardiovascular diseases and tissue inflammation due to their effect on lipid profile and blood glucose. The other ingredients in EDs may play a role in the observed metabolic disturbances. Chronic use of EDs should be especially discouraged to avoid these negative effects. PMID: 32191411 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - March 18, 2020 Category: Biochemistry Authors: Eltahir HM, Alamri G, Alamri A, Aloufi A, Nazmy M, Elbadawy H, Bahashwan S, Elbadawy HM, Alahmadi YM, Abouzied M Tags: Acta Biochim Pol Source Type: research

Prognostic value of Golgi P 73 and Beta 2-microglobulin in patients with Viral Hepatitis B.
Abstract Previous studies detected higher Golgi protein 73 levels in the serum of patients with chronic liver disease. The Beta-2 microglobulin levels were also observed to be higher in patients with chronic hepatitis B infection compared to the inactive carriers and the protein plays an important role in the response to viral infections. The aim of the present study was to assess the liver fibrosis through non-invasive methods in chronic hepatitis B patients. Three groups were included in the study. The first group comprised of the patients who were admitted to the Infectious Diseases and Clinical Microbiology cl...
Source: Acta Biochim Pol - March 18, 2020 Category: Biochemistry Authors: Deveci O, Kaplan I, Tekin R, Celen MK, Bozkurt F, Aslan E, Cerrah S, Dayan S Tags: Acta Biochim Pol Source Type: research

Dose-dependent production of linoleic acid analogues in food derived Lactobacillus plantarum K25 and in silico characterization of relevant reactions.
The objective of this study was to assess and scrutinize the competency of probiotic L. plantarum K25 to produce linoleic acid analogues in the medium supplemented with different concentrations of linoleic acid, ranging from 1% to 10%, in a dose dependent manner. The analogues produced were identified and quantitated by GC-MS and in silico studies were done to confirm enzymatic reactions involved in its conversion. The results showed that L. plantarum K25 could convert linoleic acid at different concentrations to 9 different fatty acid analogues at concentrations ranging from 0.01 to 17.24 mg/L. Among these metabolites, fo...
Source: Acta Biochim Pol - March 17, 2020 Category: Biochemistry Authors: Aziz T, Sarwar A, Fahim M, Ud Din J, Al Dalali S, Ud Din Z, Jian Z, Pacheco Fill T, Zhennai Y Tags: Acta Biochim Pol Source Type: research

Roundup-induced biochemical and histopathological changes in the liver and kidney of rats: the ameliorative effects of Linum usitatissimum oil.
Abstract The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b.w.), LuO group (0.5 g/kg b.w. of LuO) and RDP+LuO group (RDP and LuO simultaneously). LuO decreased the ferric reducing antioxidant power (FRAP) (IC50=10.36 μg/ml) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50=22.85 mg/ml) in the tested tissues. The 30-day exposure of rats to RDP caused an increase in serum hepatic and ren...
Source: Acta Biochim Pol - March 17, 2020 Category: Biochemistry Authors: Djaber N, Ounaceur LS, Moubine BN, Khaldi T, Rouag M, Berrouague S, Amara H, Taibi F, Boumendjel M, Boumendjel A, Messarah M Tags: Acta Biochim Pol Source Type: research

Hemagglutination inhibition antibody titers as a correlate of protection against influenza disease in the 2018/2019 epidemic season in Poland.
Abstract The aim of this study was to determine the level of antibodies against hemagglutinin of influenza viruses in the sera of people in the seven age groups in the epidemic season 2018/2019 in Poland. The level of anti-hemagglutinin antibodies was determined by hemagglutination inhibition test (HAI). 1050 clinical samples from all over the country were tested. The level of antibodies against influenza viruses was highest in the 10-14 age group for A/Singapore/INFIMH-16-0019/2016 (H3N2) and B/Phuket/3073/2013 Yamagata lineage antigens. These results confirm the circulation of four antigenically different influe...
Source: Acta Biochim Pol - March 16, 2020 Category: Biochemistry Authors: Hallmann-Szelińska E, Szymański K, Łuniewska K, Kondratiuk K, Brydak LB Tags: Acta Biochim Pol Source Type: research

Can VEGFR-3 be a better tumor marker for breast cancer than CA 15-3?
Abstract Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3) is a very important factor which promotes lymphangiogenesis not only in physiological but also in pathological processes in which we can include neoplasia. The aim of this study was to analyze the plasma concentrations and diagnostic utility of this parameter in comparison and in combination with CA 15-3 in breast cancer (BC) patients and in relation to the control groups. The study included 120 breast cancer and 60 control patients (28 with benign breast tumors and 32 healthy women). Plasma levels of VEGFR-3 were determined by an Enzyme-Linked Immun...
Source: Acta Biochim Pol - March 10, 2020 Category: Biochemistry Authors: Zajkowska M, Gacuta E, Lubowicka E, Szmitkowski M, Ławicki S Tags: Acta Biochim Pol Source Type: research

Downregulation of long non-coding RNA ZXF1 restricts cell survival by targeting miR-634-GRB2 in lung adenocarcinoma.
Abstract Increasing evidence demonstrates that long non-coding RNAs (lncRNAs) play important regulatory roles in mediating initiation and progression of lung adenocarcinoma (LA), which is one of the most lethal in humans. A previous study reported that lncRNAZXF1 was dysregulated in LA and enhanced expression of ZXF1 promoted the invasion and metastasis in LA. However, the effect of ZXF1 on LA progression and its underlying mechanisms were not thoroughly investigated. In our in vitro experiments, qRT-PCR revealed that the expression level of ZXF1 in LA tissues and tumor cells were significantly higher than that in...
Source: Acta Biochim Pol - March 10, 2020 Category: Biochemistry Authors: Ren X, Xu N, Zhang Y, Wang T Tags: Acta Biochim Pol Source Type: research

The effect of luteolin 7-glucoside, apigenin 7-glucoside and Succisa pratensis extracts on NF- κB activation and α-amylase activity in HepG2 cells.
The effect of luteolin 7-glucoside, apigenin 7-glucoside and Succisa pratensis extracts on NF-κB activation and α-amylase activity in HepG2 cells. Acta Biochim Pol. 2020 Mar 04;: Authors: Witkowska-Banaszczak E, Krajka-Kuźniak V, Papierska K Abstract The chemical composition of Succisa pratensis is not well known. The existing data indicate a substantial content of flavonoids, which include luteolin and apigenin 7-glucosides. The aim of this study was to elaborate the isolation protocol of these flavonoids from flowers and leaves of S. pratensis, to carry out their characterization, as we...
Source: Acta Biochim Pol - March 3, 2020 Category: Biochemistry Authors: Witkowska-Banaszczak E, Krajka-Kuźniak V, Papierska K Tags: Acta Biochim Pol Source Type: research

Genetic variability and phylogenetic analysis of Lagovirus europaeus strains GI.1 (RHDV) and GI.2 (RHDV2) based on the RNA-dependent RNA polymerase (RdRp) coding gene.
Abstract Lagovirus europaeus GI.1 (RHDV-rabbit haemorrhagic disease virus) and GI.2 (RHDV2-rabbit haemorrhagic disease virus 2), family Caliciviridae, genus Lagovirus, are etiological factors of the rabbit haemorrhagic disease (RHD). This small RNA virus is a great model for tracking the variability and evolution of RNA viruses, because it uses an RNA-dependent RNA polymerase (RdRp) to replicate its own genetic material. This polymerase determines the fidelity and the rates of replication and mutation of the virus, conditioning its adaptation to the environment and even to a new host, and thus influencing evolutio...
Source: Acta Biochim Pol - March 3, 2020 Category: Biochemistry Authors: Hukowska-Szematowicz B Tags: Acta Biochim Pol Source Type: research

The effect of D380Y pathogenic mutation in human Yin Yang 1 on the protein's structure and function.
cki A Abstract Yin Yang 1 is a human transcription factor that controls a number of genes and takes part in the regulation of cell cycle, proliferation, differentiation, and neuronal development. Yin Yang 1 is composed of an N-terminal intrinsically disordered fragment and a C-terminal domain responsible for binding to DNA, composed of four zinc fingers. Recently, various alterations in the Yin Yang 1's DNA binding domain were linked with an unexplained intellectual disability named Gabriele-de Vries syndrome. In this paper, a repetitively occurring substitution of aspartate-380 for tyrosine was analyzed to assess...
Source: Acta Biochim Pol - February 19, 2020 Category: Biochemistry Authors: Figiel M, Łakomska J, Dziedzicka-Wasylewska M, Górecki A Tags: Acta Biochim Pol Source Type: research