Vitamin D in autoimmune bullous disease.
Abstract Numerous epidemiological studies have suggested a link between vitamin D deficiency and the development of various autoimmune diseases, including diabetes mellitus type 1, rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis or systemic lupus erythematosus. More recently, such a link has been also proposed for autoimmune bullous diseases (AIBD). This is a relatively rare and potentially life-threatening, organ-specific group of inflammatory skin diseases characterized by the presence of tissue-bound and circulating autoantibodies against various molecules present in desmosomes (in pemphigu...
Source: Acta Biochim Pol - February 12, 2020 Category: Biochemistry Authors: Tukaj S Tags: Acta Biochim Pol Source Type: research

In silico characterization of linoleic acid biotransformation to rumenic acid in food derived Lactobacillus plantarum YW11.
Abstract Lactobacillus plantarum YW11 capability to convert linoleic acid into conjugated linoleic acid and other metabolites was studied in a dose-dependent manner by supplementing LA at different concentrations. L. plantarum YW11 displayed a uniform distinctive growth curve of CLA and other metabolites at concentrations of LA ranging from 1% (w/v) to 10% (w/v), with slightly increased growth at higher LA concentrations. The biotransformation capability of L. plantarum YW11 evaluated by GC-MS revealed a total of one CLA isomer, i.e. 9-cis,11-trans-octadecadienoic acid, also known as the rumenic acid (RA), one lin...
Source: Acta Biochim Pol - February 7, 2020 Category: Biochemistry Authors: Aziz T, Sarwar A, Fahim M, Al Dalali S, Ud Din Z, Ud Din J, Xin Z, Jian Z, Pacheco Fill T, Zhennai Y Tags: Acta Biochim Pol Source Type: research

The effects of clofarabine in ALL inhibition through DNA methylation regulation.
Abstract Clofarabine (2-chloro-2'-fluoro-2'-deoxyarabinosyladenine, ClF), a second-generation 2'-deoxyadenosine analog, possesses manifold anti-cancer activities. Our previous reports and some of others demonstrate the potential capacity of ClF to regulate the epigenetic machinery. The study presented here is the first to investigate the influence of ClF on modulators of the DNA methylation machinery, including DNMT1 and CDKN1A, in acute lymphoblastic leukemia (ALL) cells. ClF effects on promoter methylation and transcriptional activity of hypermethylated and silenced tumor suppressor genes (TSGs), including APC, ...
Source: Acta Biochim Pol - January 30, 2020 Category: Biochemistry Authors: Kaufman-Szymczyk A, Lubecka K Tags: Acta Biochim Pol Source Type: research

Olanzapine-mediated cardiotoxicity is associated with altered energy metabolism in isolated rat hearts.
Abstract Olanzapine is an antipsychotic drug routinely used for the treatment of schizophrenia. Although the olanzapine treatment is associated with disturbed electrical heart activity, the exact mechanism underlying this severe adverse effect remains unclear. Recently, olanzapine administration was demonstrated to be associated with elevation of blood glucose and lower levels of free fatty acids. Therefore, we investigated the effect of acute olanzapine administration on pathways regulating the cardiac energy metabolism in an isolated heart. Electrical activity and contractile parameters were recorded in isolated...
Source: Acta Biochim Pol - January 30, 2020 Category: Biochemistry Authors: Gulac P, Arnold M, Grman M, Carrel T, Longnus S, Stankovicova T, Tomasova L Tags: Acta Biochim Pol Source Type: research

Impact of developmental origin, niche mechanics and oxygen availability on osteogenic differentiation capacity of mesenchymal stem/stromal cells.
Abstract Mesenchymal Stem/Stromal Cells (MSCs) have been widely considered as a promising source of cells for tissue regeneration. Among other stem cells, they are characterized by a high osteogenic potential. Intensive studies in this field had shown that even if basic osteogenic differentiation is relatively simple, its clinical application requires more sophisticated approaches to prepare effective and safe cell therapy products. The aim of this review is to underline biological, physical and chemical factors which play a crucial role in osteogenic differentiation of MSCs. Existence of two distinct mechanisms o...
Source: Acta Biochim Pol - December 29, 2019 Category: Biochemistry Authors: Bryniarska N, Kubiak A, Łabędź-Masłowska A, Zuba-Surma E Tags: Acta Biochim Pol Source Type: research

Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase activity? A contribution to the ongoing discussion.
iek M Abstract The aim of the study presented here was an attempt to answer the question posed in the title: Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase (ALDH) activity? Here, we investigated the effect of administration (separately or jointly) of lipoic acid (LA), nitroglycerin (GTN), and disulfiram (DSF; an irreversible in vivo inhibitor of all ALDH isozymes (including ALDH2)), on the development of tolerance to GTN. We also assessed the total activity of ALDH in the rat liver homogenates. Our data revealed that not only DSF and GTN inhibited the total ALDH activity in the ...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Bilska-Wilkosz A, Kotańska M, Górny M, Filipek B, Iciek M Tags: Acta Biochim Pol Source Type: research

Sulfane sulfur - new findings on an old topic.
rny M Abstract Sulfane sulfur is a divalent sulfur atom bonded to another sulfur which is very reactive and labile. Compounds containing this reactive sulfur include persulfides, polysulfides, thiosulfate, thiosulfinates, polythionates, and elemental sulfur. Sulfane sulfur appears in a number of biologically important compounds, including thiocysteine, thiocystine and thiotaurine, products of the cysteine metabolism, as well as glutathione persulfide. Sulfane sulfur compounds can modify cysteine residues in proteins via an S-sulfhydration reaction to produce protein persulfides. It has been also postulated that cy...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Iciek M, Bilska-Wilkosz A, Górny M Tags: Acta Biochim Pol Source Type: research

An outline of the use of supramolecular compounds in biology and medicine.
Abstract Supramolecular chemistry opened up opportunities and prospects in biological studies. Numerous supramolecular systems are known which assume different shapes depending on the structure of the component molecules. Some of them are mixed systems. Ribbon-like supramolecular structures which are the main focus of this paper form complexes with proteins in a way which is a new type of interaction. Despite preserving the ribbon-like structure, it may also interact with proteins as a mixed system. In this way, it may be used as a carrier of drugs. In addition, hybrid systems created by a combination of ribbon-li...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Jagusiak A Tags: Acta Biochim Pol Source Type: research

Molecular mechanisms of epithelial to mesenchymal transition in tumor metastasis.
Abstract Epithelial to mesenchymal transition (EMT) is a process during which cancer cells lose epithelial features, cytoskeletal architecture is re-organized, cell shape changes and cells activate genes that help to define mesenchymal phenotype, what leads to an increased cell motility and dissemination of tumor to distant metastatic sites. This review describes different signaling networks between microRNAs and proteins that regulate EMT in tumor growth. Activation of EMT is mediated via series of paracrine signaling molecules. WNT, TGF-b, NOTCH and Shh signaling pathways play crucial roles in activation of EMT-...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Nieszporek A, Skrzypek K, Adamek G, Majka M Tags: Acta Biochim Pol Source Type: research

Secretion, migration and adhesion as key processes in the therapeutic activity of mesenchymal stem cells.
Abstract The MSCs are immature cells that can be found in numerous different tissue types. In recent years, they have gained considerable attention, particularly with regard to their regenerative properties. Due to their paracrine activity, ability to migrate, adhesion and homing, MSCs currently appear to be the most relevant for therapeutic use. Numerous bioactive molecules secreted by MSCs exert paracrine effects and modulate many physiological processes, such as angiogenesis, immunomodulation and neuroprotection. Cell-cell communication may be also mediated by extracellular vesicles released from the cells. Due...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Kot M, Musiał-Wysocka A, Lasota M, Ulman A, Majka M Tags: Acta Biochim Pol Source Type: research

Nanoparticles as energy donors and acceptors in bionanohybrid systems.
Abstract The bionanohybrids are the junctions of at least two objects of different origin: abiotic and biotic. The abiotic part is a nanoparticle (often a fluorescent quantum dot), the biotical one may be a protein (especially fluorescent one or redox-active one), nucleic acid, carbohydrate as well as a simple organic molecule. When such a junction undergoes illumination, the energy transfer between the partners is possible. The nanoparticles, depending on their characteristics, may be donors, acceptors or mediators of the energy transfer. In most cases, the mechanism of the transfer is the Förster resonance ...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Sławski J, Grzyb J Tags: Acta Biochim Pol Source Type: research

UV radiation in HCT 116 cells influences intracellular H2O2 and glutathione levels, antioxidant expression, and protein glutathionylation.
In this study, cells were irradiated with UV light of different wavelength (A, B, or C). A surge in H2O2 concentration and total glutathione (level occurred 6 hours later. Consequently, protein glutathionylation increased above control levels. Expression of the antioxidant enzymes: glutathione peroxidase (GPX) and glutathione reductase (GSR), assessed by real-time quantitative PCR, increased by 1.5-2 times after 24 hours post-irradiation, in comparison to the untreated controls. Glutathionylation of proteins was enhanced after UV radiation and the set of biotinylated glutathione ethyl ester (BioGEE) tagged proteins was det...
Source: Acta Biochim Pol - December 28, 2019 Category: Biochemistry Authors: Adamiec M, Skonieczna M Tags: Acta Biochim Pol Source Type: research

Conformational flexibility and ligand binding properties of ovine β-lactoglobulin.
Conformational flexibility and ligand binding properties of ovine β-lactoglobulin. Acta Biochim Pol. 2019 Dec 27;: Authors: Loch J, Bonarek P, Lewiński K Abstract Ovine β‑lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. The structure of ovine β‑lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an asymmetric dimer. The loops surrounding β-barrel in ovine lactoglobulin possessed the same conformation...
Source: Acta Biochim Pol - December 27, 2019 Category: Biochemistry Authors: Loch J, Bonarek P, Lewiński K Tags: Acta Biochim Pol Source Type: research

Novel combinatorial transdermal drug delivery of alendronate with risedronate for the treatment of osteoporosis.
Abstract The presented investigation explores the efficiency of novel transdermal drug delivery system of combination of two drugs i.e risedronate and alendronate in the treatment of osteoporosis. The nanoparticulate transdermal patch was prepared using PLGA as principle polymer which has been found to be suitable for drug delivery. The novel formulation system was found to be more efficient in lowering and maintaining the plasma calcium at a normal level when compared to a pure drug in a study carried out on an excised rat skin. PMID: 31846272 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 17, 2019 Category: Biochemistry Authors: Kong F, Jia B, Cui P, Wang D Tags: Acta Biochim Pol Source Type: research

Cladonia uncialis as a valuable raw material of biosynthetic compounds against clinical strains of bacteria and fungi.
Abstract Cladonia uncialis is a lichen species with confirmed antibacterial activity and whose genome has been recently sequenced, enabling first attempts in its functional characterization. In this work, we investigated activity of the C. uncialis acetone extract (CUE) and usnic acid (UA) enantiomers against ten clinical microbial strains causing skin infections. The results showed that CUE, containing (-)-UA and squamatic acid, assayed at the same concentrations as UA, was noticeably more active than (-)-UA alone, in its pure form. The studied CUE displayed an activity that was comparable to that of (+)-UA obser...
Source: Acta Biochim Pol - December 15, 2019 Category: Biochemistry Authors: Studzińska-Sroka E, Hanna Tomczak, Natalia Malińska, Marta Wrońska, Robert Kleszcz, Agnieszka Galanty, Judyta Cielecka-Piontek, Dorota Latek, Jarosław Paluszczak Tags: Acta Biochim Pol Source Type: research

Mesenchymal stem cells' homing and cardiac tissue repair.
Abstract Nowadays, mesenchymal stem cells (MSCs) are essential players in cellular therapy and regenerative medicine. MSCs are used to treat cardiac disorders by intramyocardial injection or injection into the bloodstream. Therefore, a premise of successful MSC-based therapy is that the cells reach the site of injury and home the damaged tissue. In response to inflammatory conditions, MSCs can potentially move into the place of injury and colonize damaged tissues, where they participate in their regeneration. This review presents the current knowledge of the mechanisms of MSCs migration and target tissue homing in...
Source: Acta Biochim Pol - December 13, 2019 Category: Biochemistry Authors: Szydlak R Tags: Acta Biochim Pol Source Type: research

Nitroxoline: repurposing its antimicrobial to antitumor application.
Abstract Cancer is a disease receiving an outstanding input of funds for basic and clinical research but is, nevertheless, still the second leading cause of death in the developed world and a great burden for health systems. New drugs are therefore needed to improve therapy, prolong survival of cancer patients and improve their quality of life. The high cost of development and clinical evaluation of new drugs limits the number that actually enter clinical use. To overcome this problem, repurposing of established drugs for new indications has gained a lot of interest, especially in the field of oncology. The well-e...
Source: Acta Biochim Pol - December 13, 2019 Category: Biochemistry Authors: Mitrović A, Kos J Tags: Acta Biochim Pol Source Type: research

Aberrant promoter methylation may be responsible for the control of CD146 (MCAM) gene expression during breast cancer progression.
In conclusion, our study revealed, for the first time, that aberrant methylation maybe involved in expression control of CD146, a very potent EMT inducer in breast cancer cells. Altogether, the data obtained may provide the basis for novel therapies as well as diagnostic approaches enabling sensitive and very accurate detection of breast cancer cells. PMID: 31826047 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 11, 2019 Category: Biochemistry Authors: Dudzik P, Trojan SE, Ostrowska B, Lasota M, Dulińska-Litewka J, Laidler P, Kocemba-Pilarczyk KA Tags: Acta Biochim Pol Source Type: research

Interaction of iron ions with melanin.
Abstract One of the antioxidant roles of melanin is binding redox-active transition metal ions. The aim of this study was to examine the redox reactions accompanying iron binding by melanin. Two kinds of synthetic eumelanin were mixed with iron (II) and iron (III) in the presence and absence of citrate and ADP in the aerobic and anaerobic system. The iron binding was examined by electron paramagnetic resonance (EPR) spectroscopy and thiocyanate assay. Obtained results indicate that although melanin reduces iron (III) that is unbound to this polymer, binding of iron (II) is accompanied by its oxidation by melanin. ...
Source: Acta Biochim Pol - December 11, 2019 Category: Biochemistry Authors: Żądło AC, Sarna T Tags: Acta Biochim Pol Source Type: research

The influence of a single and double biotinylation of xanthohumol on its anticancer activity.
Abstract Two biotinylated derivatives of the main hop chalcone xanthohumol (1) were prepared by a one-step synthesis via esterification using biotin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC×HCl) and 4-dimethylaminopyridine (DMAP) as coupling reagents. The products were characterized spectroscopically and their antiproliferative activity toward MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1 and Balb/3T3 cell lines was investigated using the SRB assay. For all three tested compounds the best activity was noted in the case of human (MCF-7) and mice (4T1) breast cancer cell lines (IC50 values
Source: Acta Biochim Pol - December 10, 2019 Category: Biochemistry Authors: Stompor M, Świtalska M, Wietrzyk J Tags: Acta Biochim Pol Source Type: research

Fluorescence of the tri-cyclic adenine and isoguanine derivatives and their ribosides: possible analytical applications.
Abstract Fluorescent tri-cyclic purine analogs, derivatives of isoguanine and adenine, were examined as potential substrates of purine-nucleoside phosphorylase. It was found previously that etheno- derivatives of both compounds are ribosylated in phosphate-free media, but ribosylation places in some instances differ from purine N9. New ribosides are examined as potential substrates of human blood PNP and indicators of this enzyme. Of these, N6-riboside of 1,N6-etheno-adenine was found the most promising. PMID: 31804790 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 5, 2019 Category: Biochemistry Authors: Stachelska-Wierzchowska A, Wierzchowski J Tags: Acta Biochim Pol Source Type: research

Effect of glycosaminoglycans accumulation on the non-oxidative sulfur metabolism in mouse model of Sanfilippo syndrome, type B.
man A Abstract Lack of the N-alpha-acetylglucosaminidase gene is responsible for the occurrence of a rare disease - the Sanfilippo syndrome, type B. The result of this gene knock-out is accumulation of glycosaminoglycans (GAGs) - more specifically heparan sulfate - a sulfate rich macromolecule. The sulfur oxidative pathway is involved in the sulfate groups' turnover in the cells. In contrast, the non-oxidative sulfur pathway leads mostly to formation of sulfane sulfur-containing compounds. The aim of our research was to observe an interaction between MPS IIIB and non-oxidative sulfur metabolism. In this work, we e...
Source: Acta Biochim Pol - December 5, 2019 Category: Biochemistry Authors: Kaczor-Kamińska M, Kamiński K, Stalińska K, Wróbel M, Feldman A Tags: Acta Biochim Pol Source Type: research

Saliva as a non-invasive material for early diagnosis.
This article describes the non-invasive and easily accessible saliva as a source of numerous molecular biomarkers. The salivary fluid can provide information about the pathological changes not only in the oral area but also in other parts of the body, therefore salivary tests may be promising tools for risk prediction and treatment monitoring of many disorders. PMID: 31799813 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - December 4, 2019 Category: Biochemistry Authors: Woźniak M, Paluszkiewicz C, Kwiatek WM Tags: Acta Biochim Pol Source Type: research

Clinical value of peripheral blood M2/M1 like monocyte ratio in the diagnosis of breast cancer and the differentiation between benign and malignant breast tumors.
Abstract BACKGROUND: During tumor progression, circulating monocytes and macrophages are actively recruited into tumors where they alter the tumor microenvironment to accelerate tumor progression. In response to multiple microenvironmental signals from the tumor and stromal cells, macrophages change their functional phenotypes. Based on their function, macrophages are commonly classified into both, classical M1 and alternative M2 macrophages. M2-like tumor-associated macrophages promote breast tumor growth and survival, and may migrate into the peripheral blood. However, the level of circulating M2/M1-like monocyt...
Source: Acta Biochim Pol - December 3, 2019 Category: Biochemistry Authors: Fadhil M, Abdul-Rasheed O, Al-Naqqash M Tags: Acta Biochim Pol Source Type: research

Role of orf73 in the development of lambdoid bacteriophages during infection of the Escherichia coli host.
Abstract Shiga toxin-producing Escherichia coli (STEC) is a group of pathogenic strains responsible for human infections that result in bloody diarrhea and hemorrhagic colitis, often with severe complications. The main virulence factors of STEC are Shiga toxins encoded by stx genes located in genomes of Shiga toxin-converting bacteriophages (Stx phages). These bacterial viruses are clustered in the lambdoid bacteriophages family represented by phage λ. Here, we report that expression of orf73 from the exo-xis region of the phage genome promotes the lysogenic pathway of development of λ and Φ24B p...
Source: Acta Biochim Pol - November 26, 2019 Category: Biochemistry Authors: Karolina Zdrojewska, Aleksandra Dydecka, Bożena Nejman-Faleńczyk, Gracja Topka, Agnieszka Necel, Węgrzyn A, Grzegorz Węgrzyn, Bloch S Tags: Acta Biochim Pol Source Type: research

SFRP1 inhibited the epithelial ovarian cancer through inhibiting Wnt/ β-catenin signaling.
CONCLUSION: SFRP1 inhibited epithelial ovarian cancer through inhibiting Wnt/β-catenin pathway, suggesting that SFRP1 could be considered as a potential therapeutic target in epithelial ovarian cancer. PMID: 31770484 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - November 26, 2019 Category: Biochemistry Authors: Zhang H, Sun D, Qiu J, Yao L Tags: Acta Biochim Pol Source Type: research

Versatility of USP18 in physiology and pathophysiology.
e;r T Abstract Ubiquitin-specific peptidase 18 (USP18) is a multifunctional protein and its roles are still being investigated. This enzyme removes ubiquitin-like molecules from their substrates and the only known interferon-stimulated gene 15 (ISG15) specific protease. Apart from its enzymatic function, it also inhibits interferon type I and III signalling pathways. USP18 is known to regulate multiple processes, such as: cell cycle, cell signalling and response to viral and bacterial infections. Moreover, it contributes to the development of several autoimmune diseases and carcinogenesis, and recently was describ...
Source: Acta Biochim Pol - November 20, 2019 Category: Biochemistry Authors: Dziamałek-Macioszczyk P, Haraźna J, Stompór T Tags: Acta Biochim Pol Source Type: research

Internal force field in selected proteins.
Abstract The fuzzy oil drop model suggests that the tertiary conformation of a protein - particularly a globular one - can be likened to a spherical micelle. During the folding process, hydrophilic residues are exposed on the surface, while hydrophobic residues are retained inside the protein. The resulting hydrophobicity distribution can be mathematically modeled as a 3D Gaussian. The fuzzy oil drop model is strikingly effective in explaining the properties of type II antifreeze proteins and fast-folding proteins, as well as a vast majority of autonomous protein domains. This work aims to determine whether simila...
Source: Acta Biochim Pol - November 20, 2019 Category: Biochemistry Authors: Ptak-Kaczor M, Banach M, Konieczny L, Roterman I Tags: Acta Biochim Pol Source Type: research

Serum chemerin level, cytokine profile and nutritional status in children with cystic fibrosis.
CONCLUSIONS: Our results show that chemerin is not associated with the nutritional status in children with cystic fibrosis. Chemerin has no impact on the levels of IL-1b, IL-6, TNFα in CF patients. IL-1b, IL6, TNFα and also IL10 are upregulated in cystic fibrosis. PMID: 31742967 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - November 14, 2019 Category: Biochemistry Authors: Sznurkowska K, Kaźmierska K, Śledziński T, Zagierski M, Liberek A, Szlagatys-Sidorkiewicz A Tags: Acta Biochim Pol Source Type: research

The The TOB/SAM complex composition in mitochondria of Dictyostelium discoideum during progression from unicellularity to multicellularity.
Abstract Despite its complex life cycle including unicellular and multicellular stages, the slime mold Dictyostelium discoideum, a well-known model in biomedical research, has not been used as a model organism in studies on mitochondrial import, including its significance in cellular processes. Moreover, data concerning mitochondrial protein import machinery in D. discoideum mitochondria is limited and nothing is known about the impact of that machinery on slime mold life cycle. Here, we focused on the TOB/SAM (topogenesis of the mitochondrial outer membrane β-barrel proteins/sorting and assembly machinery) c...
Source: Acta Biochim Pol - November 12, 2019 Category: Biochemistry Authors: Mazur M, Wojtkowska M, Skalski M, Słocińska M, Kmita H Tags: Acta Biochim Pol Source Type: research

The use of cytochrome b and ryanodine polymorphism to identify DNA of animal and human origin.
Abstract The aim of this study was to determine a match between DNA recovered from evidence material, such as knocked down red deer, and from comparative material in form of two brown traces on the bonnet of a car driven by a person suspected of knocking down the animal. The spots coming from the car provided no DNA profile, which questioned that they originated from a red deer and ruled out performance of a comparative DNA analysis. For this reason, the material obtained from the blood smear was analyzed for species identification. The method applied can discriminate between cattle, red deer and roe deer based on...
Source: Acta Biochim Pol - November 8, 2019 Category: Biochemistry Authors: Natonek-Wiśniewska M, Radko A Tags: Acta Biochim Pol Source Type: research

Light-regulated synthesis of extra- and intracellular enzymes related to wood degradation by the white rot fungus Cerrena unicolor during solid-state fermentation on ash sawdust-based medium.
Abstract The light-dependent metabolism of the white rot basidiomycete Cerrena unicolor FCL139 has already been demonstrated using transcriptomic and Biolog-based approaches. To further analyze the influence of light on C. unicolor wood degradation, we measured the activity of an array of CAZymes (carbohydrate-active enzymes) and enzymes involved in the redox system of fungal cells associated with lignolysis. Extra- and intracellular enzymatic extracts were obtained from solid-state ash sawdust C. unicolor cultures cultivated for 14 days under red, blue, green, or white light conditions, or in the dark. Light grea...
Source: Acta Biochim Pol - November 7, 2019 Category: Biochemistry Authors: Pawlik A, Jaszek M, Sulej J, Janusz G Tags: Acta Biochim Pol Source Type: research

Defective apoptosis of U937 cells induced by benzyl isothiocyanate (BITC).
Abstract Isothiocyanates precursors (ITCs), including benzyl isothiocyanate (BITC), are considered as cancer chemopreventive agents. ITC derivatives were tested in clinical trials (NCT00005883, NCT01265953, NCT01790204) and preclinical studies aimed to inhibit tumor growth and modulation of their microenvironment. Although efficacy of ITCs was demonstrated with several leukemic cell lines, the final steps of BITC-induced apoptosis were not completely elucidated in the literature. Therefore, we focused on morphological and biochemical events occurring upon treatment of U937 leukemia cells with BITC. Micromolar conc...
Source: Acta Biochim Pol - October 24, 2019 Category: Biochemistry Authors: Stasiłojć G, Nagel A, Koszałka P, Bigda JJ Tags: Acta Biochim Pol Source Type: research

Analysis of BNIP3 and BNIP3L/Nix expression in cybrid cell lines harboring two LHON-associated mutations.
Abstract Mitochondria are key players in cell death through the activation of the intrinsic apoptosis pathway. BNIP3 and BNIP3L/Nix are outer mitochondrial membrane bifunctional proteins which because of containing both BH3 and LIR domains play a role in cellular response to stress by regulation of apoptosis and selective autophagy. Leber's Hereditary Optic Neuropathy (LHON) is the most common mitochondrial disease in adults, characterized by painless loss of vision caused by atrophy of the optic nerve. The disease in over 90% of cases is caused by one of three mutations in the mitochondrial genome: 11778G>A, 3...
Source: Acta Biochim Pol - October 4, 2019 Category: Biochemistry Authors: Kodroń A, Hajieva P, Kulicka A, Paterczyk B, Jankauskaite E, Bartnik E Tags: Acta Biochim Pol Source Type: research

Genetically determined metabolism of nicotine and its clinical significance.
Abstract Enzymes of the cytochrome P-450 (CYP 450) which belong to the family of oxidase enzymes, are present in cells of all organisms and play a major role in the first phase of xenobiotic metabolism. There are several isoenzymes of CYP 450 that show differences in the speed of metabolism: poor-, extensive- and ultra-rapid. Nicotine undergoes biotransformation in the liver mainly by the CYP2A6 isoform of CYP 450. There are many polymorphic isoforms of CYP2A6 affecting the metabolism of nicotine. There are also several CYP2A6 activity inhibitors and inducers among commonly used drugs. The ability of CYP2A6 isozym...
Source: Acta Biochim Pol - October 4, 2019 Category: Biochemistry Authors: Delijewski M, Bartoń A, Delijewska P, Balwierz R, Jakubiak G, Kośmider L, Pawlas N Tags: Acta Biochim Pol Source Type: research

Effect of ZnO, TiO2, Al2O3 and ZrO2 nanoparticles on wheat callus cells.
Abstract Effect of metal oxide nanoparticles on calli of two wheat varieties: Parabola (stress tolerant) and Raweta (sensitive) was studied. ZnO induced 10% larger membrane damage in Raweta calli. TiO2, Al2O3, and ZrO2 caused nearly 30% greater lactate dehydrogenase leakage for Raweta compared to Parabola. UV-irradiation of samples containing ZnO particles intensified this effect. Membrane lipid peroxidation in ZnO treated Raweta calli was twice as high as in Parabola and further increased after UV-irradiation. TiO2, Al2O3, and ZrO2 nanoparticles caused a 4-fold increase in malondialdehyde concentration in Raweta ...
Source: Acta Biochim Pol - September 17, 2019 Category: Biochemistry Authors: Czyżowska A, Barbasz A Tags: Acta Biochim Pol Source Type: research

Functional analyses of a low-penetrance risk variant rs6702619/1p21.2 associating with colorectal cancer in Polish population.
Abstract Several studies employed the genome-wide association (GWA) analysis of single-nucleotide polymorphisms (SNPs) to identify susceptibility regions in colorectal cancer (CRC). However, the functional studies exploring the role of associating SNPs with cancer biology are limited. Herein, using chromatin immunoprecipitation assay (ChIP), reporter assay and chromosome conformation capture sequencing (3C-Seq) augmented with publically available genomic and epigenomic databases we aimed to define the function of rs6702619/1p21.2 region associated with CRC in the Polish population. Using ChIP we confirmed that rs6...
Source: Acta Biochim Pol - September 17, 2019 Category: Biochemistry Authors: Statkiewicz M, Maryan N, Kulecka M, Kuklinska U, Ostrowski J, Mikula M Tags: Acta Biochim Pol Source Type: research

The EGFR rs2233947 polymorphism is associated with lung cancer risk: a study from Jordan.
In this study, we investigated the association between EGFR rs2233947 and rs884225 SNPs and the risk of lung cancer. A total of 258 participants (129 lung cancer patients and 129 healthy controls) took part in the study. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used to genotype EGFR SNPs. A strong association was detected between rs2233947 and lung cancer (P
Source: Acta Biochim Pol - September 13, 2019 Category: Biochemistry Authors: Bashir NA, Morad FR, Khabour OF, AlFaqih MA, Khassawneh BY Tags: Acta Biochim Pol Source Type: research

In vivo studies of resveratrol contained in cosmetic emulsions.
In this study, we assessed the level of skin hydration in a group of probands who used cream with or without resveratrol. The compound has been shown to have moisturizing and tightening properties, improving the overall condition of the skin. PMID: 31518087 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 12, 2019 Category: Biochemistry Authors: Igielska-Kalwat J, Firlej M, Lewandowska A, Biedziak B Tags: Acta Biochim Pol Source Type: research

First report on echinocandin resistant Polish Candida isolates.
CONCLUSION: Our research proves that in Poland there is a problem of echinocandin resistance. Moreover, we identified two species of Candida which are rare causative agents of human infections, and there was no reported incidence of such infections in Poland until now. PMID: 31518088 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - September 12, 2019 Category: Biochemistry Authors: Mroczyńska M, Brillowska-Dabrowska A Tags: Acta Biochim Pol Source Type: research

The "exposome" concept - how environmental risk factors influence cardiovascular health.
The "exposome" concept - how environmental risk factors influence cardiovascular health. Acta Biochim Pol. 2019 Sep 10;: Authors: Daiber A, Lelieveld J, Steven S, Oelze M, Kröller-Schön S, Sørensen M, Münzel T Abstract There is general consensus that environmental pollution and non-chemical stressors contribute to the incidence and prevalence of chronic noncommunicable disease (e.g. cardiovascular, metabolic and mental). Clinical and epidemiological studies support that air pollution and traffic noise are associated with a higher risk for cardiovascular disease and sign...
Source: Acta Biochim Pol - September 10, 2019 Category: Biochemistry Authors: Daiber A, Lelieveld J, Steven S, Oelze M, Kröller-Schön S, Sørensen M, Münzel T Tags: Acta Biochim Pol Source Type: research

Cold-modulated small proteins abundance in winter triticale (x Triticosecale, Wittm.) seedlings tolerant to the pink snow mould (Microdochium nivale, Samuels and Hallett) infection.
Abstract Two winter triticale (x Triticosecale Wittmack) model cultivars: Hewo (tolerant to pink snow mould) and Magnat (sensitive) were used to test the effect of cold-hardening (4 weeks at 4°C) on soluble ≤50 kDa protein profiles of the seedling leaves. The presence and abundance of individual proteins were analysed via two-dimensional gel electrophoresis (2-DE) and Surface-Enhanced Laser Desorption/Ionization Time-of-Flight (SELDI-TOF). Up to now, no proteomics analysis of triticale response to hardening has been performed. Thus, the present paper is the first in the series describing the obtained result...
Source: Acta Biochim Pol - September 10, 2019 Category: Biochemistry Authors: Golebiowska GJ, Bonar E, Emami K, Wędzony M Tags: Acta Biochim Pol Source Type: research

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples.
Abstract An electrochemical genosensor based on an epoxy-phenanthroline-Fe(III)-NH2-ssDNA layer for the detection of RNA derived from Avian Influenza is presented. The biosensor preparation consists of: (I) modification of gold electrodes with aminoethanethiol, (II) modification of the self-assembled monolayer of aminoethanethiol with 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline using "click" chemistry, (III) a first step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (IV) a second step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (V) immobilizatio...
Source: Acta Biochim Pol - August 22, 2019 Category: Biochemistry Authors: Malecka K, Świętoń E, Verwilst P, Stachyra A, Sirko A, Dehaen W, Radecki J, Radecka H Tags: Acta Biochim Pol Source Type: research

The Oxidized proteins and activity of  the Cl-/HCО3- exchanger in erythrocytes of patients with acute alcohol intoxication.
CONCLUSIONS: These findings indicate that ethanol toxicity is manifested by alteration of oxidized protein concentration and Cl-/HCО3- exchanger activity in erythrocytes. It is hypothesized that oxidized proteins are implicated in modulation of the erythrocyte cell volume regulation. PMID: 31424711 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - August 19, 2019 Category: Biochemistry Authors: Demidchik L, Kolesnikova Y, Muravlyova L, Molotov-Luchanskiy V, Kluyev D, Bakirova R, Omarova I, Ponamareva O, Tankibaeva N, Nukhuly A Tags: Acta Biochim Pol Source Type: research

Expression of metalloproteinase 2 (MMP-2) and metalloproteinase 9 (MMP-9) in THP-1 macrophages cultured with three-dimensional titanium mini-plate systems used for surgical treatment of condylar fractures.
This study showed that the use of 3D titanium mini-plates did not alter the expression of these enzymes in THP-1 macrophages. PMID: 31368291 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - July 31, 2019 Category: Biochemistry Authors: Sikora M, Baranowska-Bosiacka I, Łukomska A, Goschorska M, Chlubek D Tags: Acta Biochim Pol Source Type: research

Two dimensional gel electrophoresis (2-DE) for high-throughput proteome analyses of Mycoplasma bovis.
Abstract Expression proteomics approaches do not only directly confirm protein coding genes of sequenced genomes but also facilitate resolution of minute qualitative protein differences and improve the quality of genome annotation. Despite development of many tools, use of 2-DE coupled with MS in proteomics is not uncommon. With an accelerated trend of genome sequencing of microorganisms, proteome analysis of animal pathogens with 2-DE has gained more attention in the last decade. Therefore, in this study primarily the protein extraction, sample preparation and loading, IPG strip rehydration, IEF, and SDS-PAGE con...
Source: Acta Biochim Pol - July 22, 2019 Category: Biochemistry Authors: Anwar Khan F, Chen X, Shoaib M, Shah M, Ahmad F, Khan H, Ur Rehman F, Saeed M, Ahmad I, Ahmad I, Guo A Tags: Acta Biochim Pol Source Type: research

Potential limitations of the Sleeping Beauty transposon use in gene expression studies.
In this study, we analysed how the Sleeping Beauty system itself influences expression of chosen genes, namely IL-6, Regnase-1 and VEGF. We found that the system alone may influence expression of IL-6. Our results indicate that Sleeping Beauty transposons should be used with caution in studies that are focused on changes in the transcript level. PMID: 31299065 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - July 12, 2019 Category: Biochemistry Authors: Sowińska W, Wawro M, Solecka A, Kasza A Tags: Acta Biochim Pol Source Type: research

Epigenetic modifiers 5-aza-2'-deoxycytidine and valproic acid differentially change viability, DNA damage and gene expression in metastatic and non-metastatic colon cancer cell lines.
In conclusion, 5-aza-dC and VPA can induce different effects in metastatic and non-metastatic cancer cell lines and this may be important in determination of epigenetic profile responsible for metastatic properties of cancer cells. PMID: 31284710 [PubMed - as supplied by publisher] (Source: Acta Biochim Pol)
Source: Acta Biochim Pol - July 9, 2019 Category: Biochemistry Authors: Ghecham A, Senator A, Pawlowska E, Bouafia W, Błasiak J Tags: Acta Biochim Pol Source Type: research

Violaxanthin conversion by recombinant diatom and plant de-epoxidases, expressed in Escherichia coli - comparative analysis.
Abstract The purpose of this research was to obtain recombinant violaxanthin de-epoxidases (VDEs) from two species. The first one was VDE of Arabidopsis thaliana (L.) Heynh. (WT Columbia strain) (AtVDE) which in vivo catalyzes conversion of violaxanthin (Vx) to zeaxanthin (Zx) via anteraxanthin (Ax). The second one was VDE of Phaeodactylum tricornutum Bohlin, 1897 (CCAP 1055/1 strain) (PtVDE) which is responsible for de-epoxidation of diadinoxanthin (Ddx) to diatoxanthin (Dtx). As the first step of our experiments, open reading frames coding for studied enzymes were amplified and subsequently cloned into pET-15b p...
Source: Acta Biochim Pol - July 7, 2019 Category: Biochemistry Authors: Olchawa-Pajor M, Bojko M, Strzałka W, Strzałka K, Latowski D Tags: Acta Biochim Pol Source Type: research

THE A water-soluble [60]fullerene-derivative stimulates chlorophyll accumulation and has no toxic effect on Chlamydomonas reinhardtii.
Abstract Chlamydomonas reinhardtii (WT 2137) P. A. Dang. (Volvocales, Chlorophyceae) is a green microalgae serving as a suitable model in scientific research and a promising industrial biotechnology platform for production of biofuel, hydrogen and recombinant proteins. Fullerenes (C60) are allotropic carbon nanoparticles discovered in 1985 and used in biomedical studies since the early 1990s, when water solubilization methodologies were developed. Recently, surface-modified hydroxylated derivatives of fullerenes were proven to enhance algal growth and drought tolerance in plants. Here, a novel type of water-solubl...
Source: Acta Biochim Pol - July 7, 2019 Category: Biochemistry Authors: Lang J, Melnykova M, Catania M, Inglot A, Zyss A, Mikruta K, Firgolska D, Wieremiejczuk A, Książek I, Serda M, Nalepa P, Pluciński B, Giza A, Jedynak P Tags: Acta Biochim Pol Source Type: research