Query: siRNA

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Total 13814 results found since Jan 2013.

MicroRNA-137 dysregulation predisposes to osteoporotic fracture by impeding ALP activity and expression via suppression of leucine-rich repeat-containing G-protein-coupled receptor 4 expression.
In conclusion, these data prove that the overexpression of miR-137 was associated with an altered risk of fracture in patients with osteoporosis, and can be used as a biomarker for the prediction of risk of fracture in osteoporosis. PMID: 29786747 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - May 17, 2018 Category: Molecular Biology Authors: Liu X, Xu X Tags: Int J Mol Med Source Type: research

Design of PEI-conjugated bio-reducible polymer for efficient gene delivery
Publication date: 10 July 2018 Source:International Journal of Pharmaceutics, Volume 545, Issues 1–2 Author(s): Joung-Pyo Nam, Soyoung Kim, Sung Wan Kim The poly(cystaminebis(acrylamide)-diaminohexane) (poly(CBA-DAH)) was designed previously as a bio-reducible efficient gene delivery carrier. However, the high weight ratio required to form the polyplexes between poly(CBA-DAH) with pDNA is still a problem that needs to be addressed. To solve this problem and increase the transfection efficiency, poly(ethylenimine) (PEI, 1.8 kDa) was conjugated to poly(CBA-DAH) via disulfide bond. The PEI conjugated poly(CBA-DAH) (PCDP)...
Source: International Journal of Pharmaceutics - May 16, 2018 Category: Drugs & Pharmacology Source Type: research

G-protein Pathway Suppressor 2 (GPS2) enhanced the renal large conductance Ca2+ activated potassium channel expression via inhibiting ERK 1/2 signaling pathway.
In this study, we investigated the effects of GPS2 on BK channel activity and protein expression. In HEK BK stably-expressing cells transfected with either GPS2 or its pCMV vector control, a single cell recording showed that GPS2 significantly increased BK channel activity (NPo), increasing BK open probability (Po) and channel number (N) compared to the control. In Cos-7 cells and HEK293T cells, GPS2 overexpression significantly enhanced the total protein expression of BK in a dose-dependent manner. Knockdown of GPS2 expression significantly decreased BK protein expression while increasing ERK1/2 phosphorylation. Knockdown...
Source: Am J Physiol Renal P... - May 16, 2018 Category: Urology & Nephrology Authors: Zhuang Z, Xiao J, Chen X, Hu X, Li R, Chen S, Feng X, Shen S, Ma HP, Zhuang J, Cai H Tags: Am J Physiol Renal Physiol Source Type: research

Inhibition of Nogo-B promotes cardiac hypertrophy via endoplasmic reticulum stress
Publication date: August 2018 Source:Biomedicine & Pharmacotherapy, Volume 104 Author(s): Junli Li, Wenchao Wu, Yanguo Xin, Mingyue Zhao, Xiaojing Liu Aims Nogo-B is a key endoplasmic reticulum (ER) protein that regulates ER stress signaling. However, its role in cardiac hypertrophy remains poorly understood. ER stress is interrelated with autophagy in the process of cardiac hypertrophy. Therefore, we aimed to test the hypothesis that both ER stress and autophagy signaling mediate the function of Nogo-B in cardiac hypertrophy. Main methods Rat models of transverse aortic constriction (TAC), neonatal rat cardiomyoc...
Source: Biomedicine and Pharmacotherapy - May 16, 2018 Category: Drugs & Pharmacology Source Type: research

Pulmonary delivery of polyplexes for combined PAI-1 gene silencing and CXCR4 inhibition to treat lung fibrosis
This report describes the development of polyplexes based on CXCR4-inhibiting poly(ethylenimine) derivative (PEI-C) for pulmonary delivery of siRNA to silence plasminogen activator inhibitor-1 (siPAI-1) as a new combination treatment of pulmonary fibrosis (PF). Safety and delivery efficacy of the PEI-C/siPAI-1 polyplexes was investigated in vitro in primary lung fibroblasts isolated from mice with bleomycin-induced PF. Biodistribution analysis following intratracheal administration of fluorescently labeled polyplexes showed prolonged retention in the lungs. Treatment of mice with bleomycin-induced PF using the PEI-C/siPAI-...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 16, 2018 Category: Nanotechnology Source Type: research

Amelioration of myocardial ischemia-reperfusion injury by SIRT4 involves mitochondrial protection and reduced apoptosis.
Abstract Apoptosis and mitochondria dysfunction are key contributors to myocardial ischemia-reperfusion (MI-R) injury. SIRT4, a mitochondrial-localized sirtuin, controls cellular energy metabolism and stress response, and is abundantly present in the heart, however, its role in MI-R injury is not clear. In the current study, we demonstrate that SIRT4 is downregulated in cardiomyocytes both in vitro and in vivo models after MI-R. Functionally, SIRT4 overexpression decreases myocardial infarct size and serum creatine phosphokinase (CPK) level, and vice versa, SIRT4 depletion by siRNA increases myocardial infarct siz...
Source: Biochemical and Biophysical Research communications - May 16, 2018 Category: Biochemistry Authors: Zeng G, Liu H, Wang H Tags: Biochem Biophys Res Commun Source Type: research

Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1 ‑tP proteins.
Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. Int J Mol Med. 2018 May 16;: Authors: Zeng Y, Li Z, Shang J, Kang Y Abstract Hepatitis B virus (HBV) infection and related liver complications remain severe public health problems worldwide. Previous investigations have shown that small interfering (si)RNAs can offer an effective strategy for the treatment of chronic hepatitis B. The present study aimed to develop a novel siRNA‑delivering system of therapeutic HBV nuclear localization sequence (NLS) siRNAs using the recombinan...
Source: International Journal of Molecular Medicine - May 16, 2018 Category: Molecular Biology Authors: Zeng Y, Li Z, Shang J, Kang Y Tags: Int J Mol Med Source Type: research

Asymmetric dimethylarginine contributes to retinal neovascularization of diabetic retinopathy through EphrinB2 pathway.
In conclusion, ADMA contributes to the neovascularization of retina in diabetic mellitus, which is regulated by ephrinB2. PMID: 29777874 [PubMed - as supplied by publisher]
Source: Vascular Pharmacology - May 16, 2018 Category: Drugs & Pharmacology Authors: Du MR, Yan L, Li NS, Wang YJ, Zhou T, Jiang JL Tags: Vascul Pharmacol Source Type: research

COP9 signalosome subunit 5 regulates cancer metastasis by deubiquitinating SNAIL.
In this study, we identified COP9 signalosome subunit 5 (COPS5) as a deubiquitinating enzyme of SNAIL by using siRNA library screening. COPS5 downregulation significantly reduced the expression of SNAIL and impaired the metastatic potential of lung cancer cells both in vitro and in vivo. Importantly, we demonstrated that COPS5 binds to SNAIL and stabilizes its expression by deubiquitination. Furthermore, we observed the positive correlation between COPS5 and SNAIL expression in the clinical tissue samples of lung adenocarcinomas by using tissue microarray analysis. These findings provide strong evidence that COPS5 can be a...
Source: Oncotarget - May 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Inhibition of mTOR complex 2 restrains tumor angiogenesis in multiple myeloma.
Authors: Lamanuzzi A, Saltarella I, Desantis V, Frassanito MA, Leone P, Racanelli V, Nico B, Ribatti D, Ditonno P, Prete M, Solimando AG, Dammacco F, Vacca A, Ria R Abstract The mammalian Target of Rapamycin (mTOR) is an intracellular serine/threonine kinase that mediates intracellular metabolism, cell survival and actin rearrangement. mTOR is made of two independent complexes, mTORC1 and mTORC2, activated by the scaffold proteins RAPTOR and RICTOR, respectively. The activation of mTORC1 triggers protein synthesis and autophagy inhibition, while mTORC2 activation promotes progression, survival, actin reorganization...
Source: Oncotarget - May 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The clinicopathological significance of monocarboxylate transporters in testicular germ cell tumors.
Conclusion: The proteins herein studied, with the exception of MCT2, were associated with characteristics of worse prognosis, lower global and event free survival of patients with GCTs. Also, in vitro MCT4 silencing stimulated cell migration and invasion. Materials and Methods: Immunohistochemical expression was evaluated on samples from 149 adult patients with testicular GCT, arranged in Tissue Microarrays (TMAs), and associated with the clinicopathological data. Also, MCT4 silencing studies using siRNA were performed in JEG-3 cells. PMID: 29755659 [PubMed]
Source: Oncotarget - May 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

A Succinate Ether Derivative of Tocotrienol Enhances Dickkopf-1 Gene Expression through Epigenetic Alterations in Malignant Mesothelioma Cells
Conclusions: Targeting the epigenetic induction of DKK1 may lead to effective treatment of MM, and T3E has great potential to induce anti-MM activity.Pharmacology 2018;102:26 –36
Source: Pharmacology - May 15, 2018 Category: Drugs & Pharmacology Source Type: research

Kruppel-like factor 4 (KLF-4) plays a crucial role in simvastatin (SVT)-induced differentiation of rabbit articular chondrocytes.
This study indicates that KLF-4 plays critical role in SVT-caused chondrocytes differentiation. PMID: 29775609 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - May 15, 2018 Category: Biochemistry Authors: Yu SM, Kim SJ Tags: Biochem Biophys Res Commun Source Type: research

Long noncoding RNAs exchange during zygotic genome activation in goat.
Abstract In mammals, their proper development during the early cleavage stages strongly relies on the gene products newly transcribed by zygotic genome activation (ZGA). Long noncoding RNAs (lncRNAs) have been characterized as key regulators of the ZGA process in mice and human. However, the ZGA stage has not yet been identified and epigenetic regulations of the ZGA process remain largely unknown in goats. Here, we show that ZGA occurred at the 8-cell stage in goats. During ZGA, tri-methylation of H3K9 was dynamically changed but maintained strong staining in development arrested embryos. Using single-cell RNA seq...
Source: Biology of Reproduction - May 15, 2018 Category: Reproduction Medicine Authors: Deng M, Wan Y, Liu Z, Ren C, Zhang G, Pang J, Zhang Y, Wang F Tags: Biol Reprod Source Type: research

TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.
In conclusion, loss of TRIM22 suppresses the progression and invasion of CML through regulation of PI3K/Akt/mTOR pathway, suggesting that TRIM22 might be as a potential target for the treatment strategy of CML. PMID: 29762880 [PubMed - as supplied by publisher]
Source: Cell Biology International - May 15, 2018 Category: Cytology Authors: Li L, Qi Y, Ma X, Xiong G, Wang L, Bao C Tags: Cell Biol Int Source Type: research

Overexpression of Pyruvate Kinase Type M2 (PKM2) Promotes Ovarian Cancer Cell Growth and Survival Via Regulation of Cell Cycle Progression Related with Upregulated CCND1 and Downregulated CDKN1A Expression.
CONCLUSIONS PKM2 may play important roles in ovarian cancer development and may be a treatment target for this cancer. PMID: 29752805 [PubMed - in process]
Source: Medical Science Monitor - May 14, 2018 Category: Research Tags: Med Sci Monit Source Type: research

TGF- β1 induced fascin1 expression facilitates the migration and invasion of kidney carcinoma cells through ERK and JNK signaling pathways.
In conclusion, these results reveal that fascin1 is an important mediator of TGF-β1-induced invasion and migration of KCC through ERK and JNK signal pathways. PMID: 29772238 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - May 14, 2018 Category: Biochemistry Authors: Yang J, Zhang N, Gao R, Zhu Y, Zhang Z, Xu X, Wang J, Li Z, Liu X, Li Z, Li J, Bi J, Kong C Tags: Biochem Biophys Res Commun Source Type: research

Autophagy mediates enhancement of proangiogenic activity by hypoxia in mesenchymal stromal/stem cells.
Abstract Mesenchymal stromal/stem cells (MSCs) have been promising source for regenerative cell therapy in ischemic diseases. To improve efficacy of MSC therapy, various priming methods have been developed, and hypoxic priming has been reported to enhance therapeutic efficacy of MSCs by increasing secretion level of growth factors and cytokines. Recently, it has been reported that bone marrow MSCs primed with hypoxic condition show an increase of autophagy. Here, we addressed whether proangiogenic activity increased by hypoxic condition is associated with autophagy. Wharton's jelly derived MSCs primed with hypoxia...
Source: Biochemical and Biophysical Research communications - May 14, 2018 Category: Biochemistry Authors: Lee SG, Joe YA Tags: Biochem Biophys Res Commun Source Type: research

Reticulon 3 Regulates VLDL Secretion by Controlling VTV Biogenesis.
Abstract Secretion of very low-density lipoprotein (VLDL) by the liver is an important physiological process; however, the rate of VLDL secretion is determined by its transport from the ER to the Golgi. This transport event is facilitated by a specialized ER-derived vesicle, the VLDL transport vesicle (VTV). We have reported earlier a detailed VTV proteome which revealed that reticulon 3 (RTN3) is uniquely present in the VTV. Our immunoblotting and electron microscopic data demonstrate that RTN3 is enriched in the VTV; however, other ER-derived vesicles do not contain RTN3. Co-immunoprecipitation data coupled with...
Source: Canadian Journal of Physiology and Pharmacology - May 14, 2018 Category: Drugs & Pharmacology Authors: Siddiqi S, Zhelyabovska O, Siddiqi SA Tags: Can J Physiol Pharmacol Source Type: research

NGF and PI3K/Akt signaling participate in the ventral motor neuronal protection of curcumin in sciatic nerve injury rat models
Conclusion Our findings revealed that curcumin exerts its protective effect against injured neurons through stimulating NGF release which further activates TrkA and PI3K/Akt cell survival signaling.
Source: Biomedicine and Pharmacotherapy - May 14, 2018 Category: Drugs & Pharmacology Source Type: research

CDK16 overexpressed in non-small cell lung cancer and regulates cancer cell growth and apoptosis via a p27-dependent mechanism
Publication date: July 2018 Source:Biomedicine & Pharmacotherapy, Volume 103 Author(s): Hongtao Wang, Hongli Liu, Shengping Min, Yuanbing Shen, Wei Li, Yuqing Chen, Xiaojing Wang Cyclin-dependent kinase 16 (CDK16, PCTAIRE1) expression is upregulated in a wide variety of human malignancies. However, the function(s) of CDK16 in non-small cell lung cancer (NSCLC) remain unknown. Therefore, here we investigated the role of CDK16 in NSCLC. From 43 NSCLC tumors and matching healthy control lung tissues, immunohistochemistry revealed significantly greater CDK16 and phospho-p27Ser10 staining levels in NSCLC samples relati...
Source: Biomedicine and Pharmacotherapy - May 14, 2018 Category: Drugs & Pharmacology Source Type: research

Targeted-gene silencing of BRAF to interrupt BRAF/MEK/ERK pathway synergized photothermal therapeutics for melanoma using a novel FA-GNR-siBRAF nanosystem
Publication date: Available online 21 April 2018 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Yujuan Zhang, Xuelin Zhan, Shanshan Peng, Ying Cai, Yu Shrike Zhang, Yanling Liu, Zhigang Wang, Yanrong Yu, Yifan Wang, Qiaofa Shi, Xiaoping Zeng, Keng Yuan, Nanjin Zhou, Rakesh Joshi, Meng Zhang, Zhuxu Zhang, Weiping Min Melanoma is significantly associated with mutant BRAF gene, a suitable target for siRNA-based anti-melanoma therapy. However, a tumor-specific delivery system is a major hurdle for clinical applications. Here, we developed a novel nano-carrier, FA-GNR-siBRAF for safe topical application, ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 13, 2018 Category: Nanotechnology Source Type: research

ClC-3 chloride channel protein induces G1  arrest in hepatocellular carcinoma Hep3B cells.
In conclusion, we demonstrated that ClC-3 can arrest the cell cycle at the G1 phase to inhibit cell proliferation, suggesting that ClC-3 has the potential to be a novel target for HCC therapy and potentially improve the prognosis of HCC patients. PMID: 29749557 [PubMed - as supplied by publisher]
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Suppression of RRM2 inhibits cell proliferation, causes cell cycle arrest and promotes the apoptosis of human neuroblastoma cells and in human neuroblastoma RRM2 is suppressed following chemotherapy.
Authors: Li J, Pang J, Liu Y, Zhang J, Zhang C, Shen G, Song L Abstract Ribonucleotide reductase regulatory subunit M2 (RRM2) is a rate‑limiting enzyme for DNA synthesis and repair. RRM2 has vital roles in controlling the progression of cancer. In the present study, we investigated the RRM2 level in neuroblastoma tissues, analyzed its relationship with clinicopathological characteristics of neuroblastoma patients, and explored the effect of RRM2 on the biological functions of neuroblastoma cells. RRM2 levels in 67 pairs of neuroblastoma and matched adjacent non‑cancerous tissues were detected by qRT‑PCR, and...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma.
Authors: Bi C, Jiang B Abstract The morbidity of colorectal cancer (CRC) increases annualy, which accounts to higher mortality worldwide. Therefore, it is important to study the pathogenesis of colon cancer. Ribophorin II (RPN2), part of the N-oligosaccharyltransferase complex, is highly expressed in CRC. In the present study, we investigated whether RPN2 can regulate apoptosis, migration and invasion by RNA interference in CRC and sought to clarify the molecular mechanism involved. Based on previous research, an abnormal high expression of RPN2 was observed in CRC tissues and cell lines by real-time (RT)-PCR, im...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Protein arginine methyltransferase 5 is implicated in the aggressiveness of human hepatocellular carcinoma and controls the invasive activity of cancer cells.
Authors: Jeon JY, Lee JS, Park ER, Shen YN, Kim MY, Shin HJ, Joo HY, Cho EH, Moon SM, Shin US, Park SH, Han CJ, Choi DW, Gu MB, Kim SB, Lee KH Abstract Protein arginine methyltransferase 5 (PRMT5) is a protein that catalyzes transfer of methyl groups to the arginine residues of proteins and is involved in diverse cellular and biological responses. While the participation of PRMT5 in cancer progression has been increasingly documented, its association with the invasive phenotype currently remains poorly understood. In the present study, we revealed that PRMT5 is overexpressed in human hepatocellular carcinoma (HCC...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells
Publication date: 5 August 2018 Source:Journal of Hazardous Materials, Volume 355 Author(s): Qianqian Deng, Guanmin Jiang, Yingmin Wu, Jiexin Li, Weiting Liang, Likun Chen, Qiao Su, Wuguo Li, Jun Du, Chris K.C. Wong, Zhuojia Chen, Hongsheng Wang Nowadays, risk factors of triple-negative breast cancer (TNBC) metastasis are not well identified. Our present study reveals that an industrial chemical, bisphenol S (BPS), can promote the migration, but not the proliferation, of TNBC cells in vitro. BPS activates YAP, a key effector of Hippo pathway, by inhibiting its phosphorylation, which promotes YAP nuclear accumulation and u...
Source: Journal of Hazardous Materials - May 12, 2018 Category: Environmental Health Source Type: research

Sulforaphane Promotes Mitochondrial Protection in SH-SY5Y Cells Exposed to Hydrogen Peroxide by an Nrf2-Dependent Mechanism
AbstractSulforaphane (SFN; C6H11NOS2) is an isothiocyanate found in cruciferous vegetables, such as broccoli, kale, and radish. SFN exhibits antioxidant, anti-apoptotic, anti-tumor, and anti-inflammatory activities in different cell types. However, it was not previously demonstrated whether and how this natural compound would exert mitochondrial protection experimentally. Therefore, we investigated here the effects of a pretreatment (for 30  min) with SFN at 5 μM on mitochondria obtained from human neuroblastoma SH-SY5Y cells exposed to hydrogen peroxide (H2O2) at 300  μM for 24 h. We found that SFN prevented loss of...
Source: Molecular Neurobiology - May 11, 2018 Category: Neurology Source Type: research

Effects of dexamethasone on C6 cell proliferation, migration and invasion through the upregulation of AQP1.
Authors: Guan Y, Chen J, Zhan Y, Lu H Abstract Dexamethasone (Dex) is commonly used to treat glioma; however, the mechanism underlying the action of Dex remains unclear. In the present study, the hypothesis that aquaporin-1 (AQP1) may participate in tumor cell proliferation, apoptosis, migration and invasion was tested using small interfering RNA (siRNA). The results of the current study indicated that Dex could inhibit the proliferation, in addition to promoting the migration, of C6 cells. Dex was indicated to promote the expression of AQP1. Downregulation of AQP1, achieved using siRNAs, demonstrated the inhibitio...
Source: Oncology Letters - May 11, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Cancer treatment developed at Ben-Gurion U. changes cancer cells into normal ones
(American Associates, Ben-Gurion University of the Negev) " Using the siRNA treatment for several types of cancer in mouse models reprogramed cancer cell metabolism, reduced tumor growth and angiogenesis, reduced tumor invasiveness, and induced cancer stem cell disappearance and cell differentiation, " says BGU Prof. Shoshan-Barmatz.
Source: EurekAlert! - Medicine and Health - May 11, 2018 Category: International Medicine & Public Health Source Type: news

ASAP New Formulation for the Delivery of Oligonucleotides Using “Clickable” siRNA-Polyisoprenoid-Conjugated Nanoparticles: Application to Cancers Harboring Fusion Oncogenes
Bioconjugate ChemistryDOI: 10.1021/acs.bioconjchem.8b00205
Source: Bioconjugate Chemistry - May 11, 2018 Category: Biochemistry Authors: Liliane Massaad-Massade, Suzan Boutary, Marie Caillaud, Celine Gracia, Beatrice Parola, Soukaina Bel Gnaouiya, Barbara Stella, Silvia Arpicco, Eric Buchy, Didier Desma ële, Patrick Couvreur, Giorgia Urbinati Source Type: research

Expression of GRIM-19 in unexplained recurrent spontaneous abortion and possible pathogenesis
AbstractSTUDY QUESTIONIs aberrant expression of gene associated with retinoid-interferon-induced mortality-19 (GRIM-19) associated with unexplained recurrent spontaneous abortion (URSA)?SUMMARY ANSWERGRIM-19 deficiency may regulate regulatory T cell/T helper 17 cell (Treg/Th17) balance partly through reactive oxygen species (ROS) –mammalian target of rapamycin (mTOR) signaling axis in URSA.WHAT IS KNOWN ALREADYImmunological disorders may cause impaired maternal immune tolerance to the fetus and result in fetal rejection. The differentiation of Treg and Th17 cells is controlled by phosphoinositide 3-kinase (PI3K)/Akt/mTOR...
Source: Molecular Human Reproduction - May 11, 2018 Category: Molecular Biology Source Type: research

Effect of Bmi-1-mediated NF- κB signaling pathway on the stem-like properties of CD133+ human liver cancer cells.
CONCLUSION: Down-regulating Bmi-1 may inhibit the biological properties of CD133+ LCSC by blocking NF-κB signaling pathway, which lays a scientific foundation for the clinical treatment of liver cancer. PMID: 29843222 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers : Section A of Disease Markers - May 11, 2018 Category: Cancer & Oncology Authors: Ma DQ, Zhang YH, Ding DP, Li J, Chen LL, Tian YY, Ao KJ Tags: Cancer Biomark Source Type: research

Inhibiting EZH2 rescued bupivacaine-induced neuronal apoptosis in spinal cord dorsal root ganglia in mice
ConclusionEZH2 is upregulated, whereas inhibiting EZH2 exerted rescuing effect in anesthetics (bupivacaine)-induced spinal cord DRG. The possible downstream target of EZH2 inhibition may interact with caspase and TrkC signaling pathways.
Source: Journal of Anesthesia - May 11, 2018 Category: Anesthesiology Source Type: research

GDNF family receptor α-1 in the catfish: Possible implication to brain dopaminergic activity.
Abstract Glial cell line-derived neurotrophic factor (GDNF) is a potent trophic factor that preferentially binds to GDNF family receptor α-1 (GFRα-1) by regulating dopaminergic (DA-ergic) neurons in brain. Present study aimed to evaluate the significance of GFRα-1 expression during early brain development in catfish. Initially, the full-length cDNA of GFRα-1 was cloned from adult brain which showed high homology with other vertebrate counterparts. Quantitative PCR analysis of tissue distribution revealed ubiquitous expression of GFRα-1 in the tissues analyzed with high levels in female brain and ovary. Signif...
Source: Brain Research Bulletin - May 11, 2018 Category: Neurology Authors: Mamta SK, Senthilkumaran B Tags: Brain Res Bull Source Type: research

Anti-inflammatory effect of the extracts from the branch of Taxillus yadoriki being parasitic in Neolitsea sericea in LPS-stimulated RAW264.7 cells
In this study, we evaluated the anti-inflammatory effect of extracts of branch from Taxillus yadoriki being parasitic in Neolitsea sericea (TY-NS-B) using in vitro model. TY-NS-B significantly inhibited LPS-induced secretion of NO and PGE2 in RAW264.7 cells. TY-NS-B was also observed to inhibit LPS-mediated iNOS COX-2 expression. In addition, TY-NS-B attenuated production of inflammatory cytokines such as TNF-α and IL-1β induced by LPS. TY-NS-B blocked LPS-mediated inhibitor of IκB-α, and inhibited p65 translocation to the nucleus and NF-κB activation. Furthermore, TY-NS-B reduced the phosphorylation of MAPKs such as ...
Source: Biomedicine and Pharmacotherapy - May 11, 2018 Category: Drugs & Pharmacology Source Type: research

Effect of Wnt/ β-catenin signal pathway on of matrix metalloproteinase-7 and vascular endothelial growth factor gene expressions in endometriosis.
CONCLUSION: Blocking of Wnt/β-catenin signal pathway caused the decrease of MMP-7 and VEGF expressions, in- dicating that Wnt/β-catenin signal pathway plays an important role in the adhesion, invasion, and metastasis of endometriosis tissues. PMID: 29734552 [PubMed]
Source: Clinical and Experimental Obstetrics and Gynecology - May 10, 2018 Category: OBGYN Tags: Clin Exp Obstet Gynecol Source Type: research

Bromodomain protein BRD4 is increased in human placentas from women with early-onset preeclampsia
In conclusion, these findings suggest BRD4 may play a central role in propagating inflammation and endothelial dysfunction associated with the pathophysiology of early-onset preeclampsia.
Source: Reproduction - May 10, 2018 Category: Reproduction Medicine Authors: Liong, S., Barker, G., Lappas, M. Tags: Research Source Type: research

IGF2BP1 over-expression in skin squamous cell carcinoma cells is essential for cell growth.
Abstract The present study examined expression and potential functions of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in human skin squamous cell carcinoma (SCC). We show that IGF2BP1 mRNA and protein expression levels were upregulated in established (A431 line) and primary human skin SCC cells. Its expression was also increased in human skin SCC tissues, as compared to the normal skin tissues. In skin SCC cells, IGF2BP1 silencing or CRISPR/Cas9 knockout decreased levels of IGF2BP1-stablized mRNAs, including IGF2, CD44, Gli1 and Myc. Furthermore, skin SCC cell survival and proliferation were in...
Source: Biochemical and Biophysical Research communications - May 10, 2018 Category: Biochemistry Authors: Liu Z, Wu G, Lin C, Guo H, Xu J, Zhao T Tags: Biochem Biophys Res Commun Source Type: research

Pulmonary artery smooth muscle cell HIF-1 α regulates endothelin expression via microRNA-543.
Pulmonary artery smooth muscle cell HIF-1α regulates endothelin expression via microRNA-543. Am J Physiol Lung Cell Mol Physiol. 2018 May 10;: Authors: Wang CC, Ying L, Barnes EA, Adams ES, Kim FY, Engel KW, Alvira CM, Cornfield DN Abstract Pulmonary artery smooth muscle cells (PASMC) express endothelin (ET-1) which modulates the pulmonary vascular response to hypoxia. Although cross-talk between hypoxia-inducible factor-1α (HIF-1α), an O2-sensitive transcription factor, and ET-1 is established, the cell-specific relationship between HIF-1α and ET-1 expression remains incompletely understood. We t...
Source: Am J Physiol Lung Ce... - May 10, 2018 Category: Respiratory Medicine Authors: Wang CC, Ying L, Barnes EA, Adams ES, Kim FY, Engel KW, Alvira CM, Cornfield DN Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Filaggrin and tight junction proteins are crucial for IL-13-mediated esophageal barrier dysfunction.
Abstract Eosinophilic esophagitis (EoE) is an allergy-mediated disease that is accompanied by IL-13 overexpression and an impaired esophageal barrier. Filaggrin (FLG) and tight junction (TJ) proteins are considered to contribute to epithelial barrier function. However, their functional involvement in EoE has not been elucidated. Here, we aimed to determine the IL-13-mediated barrier dysfunction and expression of TJ-related proteins in EoE, and to characterize interactions among TJ-related proteins involved in the barrier function of the esophageal epithelium. Biopsy specimens from EoE patients were analyzed. Prima...
Source: Am J Physiol Gastroi... - May 10, 2018 Category: Gastroenterology Authors: Wu L, Oshima T, Li M, Tomita T, Fukui H, Watari J, Miwa H Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research

Androgen receptor: a potential therapeutic target for glioblastoma.
Authors: Zalcman N, Canello T, Ovadia H, Charbit H, Zelikovitch B, Mordechai A, Fellig Y, Rabani S, Shahar T, Lossos A, Lavon I Abstract The median survival time of patients with glioblastoma is still poor (14.6 month), partly due to a lack of effective treatment. We have observed that androgen receptor (AR) is amplified in glioblastomas at the DNA, RNA and protein levels. The AR gene was amplified in 27% of glioblastoma specimens from men (n=22) and of 38.2% from women (n=21). AR-RNA was overexpressed (>2.5 fold) in 93% (n=30), and AR-protein was induced (>two fold) in 56% of the glioblastomas samples (n=16)...
Source: Oncotarget - May 9, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Gamma-Klotho exhibits multiple roles in tumor growth of human bladder cancer.
Authors: Hori S, Miyake M, Tatsumi Y, Morizawa Y, Nakai Y, Onishi S, Onishi K, Iida K, Gotoh D, Tanaka N, Fujimoto K Abstract Alpha-Klotho (KLα) and beta-Klotho (KLβ) have recently been reported to correlate with cancer prognosis in some malignancies and we previously reported the association between KLα, KLβ, and urothelial carcinoma of the bladder (UCB), indicating that KLβ acts as a tumor promoter. However, the association between gamma-Klotho (KLγ) and cancer prognosis remains unclear. In the present study, we evaluated the association between KLγ and UCB. To evaluate the effect of KLγ on human bladder ...
Source: Oncotarget - May 9, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

FG-4592 Accelerates Cutaneous Wound Healing by Epidermal Stem Cell Activation via HIF-1 α Stabilization
Conclusion: This study demonstrated that both hypoxia and exogenous FG-4592 improve EpSC proliferation and motility by stabilizing HIF-1 α, and its results suggest that HIF-1α is an important target through which wound healing can be accelerated and that FG-4592 is a promising new drug for wound repair.Cell Physiol Biochem 2018;46:2460 –2470
Source: Cellular Physiology and Biochemistry - May 9, 2018 Category: Cytology Source Type: research

Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/ β-catenin signaling pathway and the epithelial-mesenchymal transition process.
This study aimed to explore the effect of MED27 on the expression of epithelial-mesenchymal transition (EMT)-related proteins and β-catenin in adrenal cortical carcinoma (ACC). The functional mechanism of MED27 on ACC processes was also explored. The expression of MED27 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR). siRNA was utilized to knockdown the expression of MED27. CCK8 assays were performed to evaluate SW-13 cell proliferation. Transwell assays were performed to assess the invasion ability, and wound healing assays were utilized to detect migration. A tumor xenograft mouse model was es...
Source: Biological Chemistry - May 9, 2018 Category: Chemistry Tags: Biol Chem Source Type: research

The role of microRNA-1 targeting MAPK3 in myocardial ischemia/reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway.
Abstract Recent studies have uncovered the vital roles played by microRNAs (miRNAs) in regulating cardiac injury. Amongst them, the cardiac enriched microRNA-1 (miR-1) has been extensively studied and proven to be detrimental to cardiac myocytes. Hence, the current study aims to explore whether miR-1 affects myocardial ischemia/reperfusion injury (MIRI) in rats undergoing sevoflurane preconditioning and the underlying mechanism. After successful model establishment, rats with MIRI were transfected with mimics or inhibitors of miR-1, or siRNA against MAPK3, and then were injected with sevoflurane. A luciferase repo...
Source: Am J Physiol Cell Ph... - May 9, 2018 Category: Cytology Authors: Hao YL, Fang HC, Zhao HL, Li XL, Luo Y, Wu BQ, Fu MJ, Liu W, Liang JJ, Chen XH Tags: Am J Physiol Cell Physiol Source Type: research

MicroRNA-150 restores endothelial cell function and attenuates vascular remodeling by targeting PTX3 through the NF- κB signaling pathway in mice with acute coronary syndrome.
MicroRNA-150 restores endothelial cell function and attenuates vascular remodeling by targeting PTX3 through the NF-κB signaling pathway in mice with acute coronary syndrome. Cell Biol Int. 2018 May 09;: Authors: Luo XY, Zhu XQ, Li Y, Wang XB, Yin W, Ge YS, Ji WM Abstract MicroRNAs have been known to function as important regulators in the vascular system, with various physiopathological effects such as vascular remodeling and hypertension modulation. We aimed to explore whether microRNA-150 (miR-150) regulates endothelial cell function and vascular remodeling in acute coronary syndrome (ACS), and th...
Source: Cell Biology International - May 9, 2018 Category: Cytology Authors: Luo XY, Zhu XQ, Li Y, Wang XB, Yin W, Ge YS, Ji WM Tags: Cell Biol Int Source Type: research

LncRNA uc.48+ is involved in the diabetic immune and inflammatory responses mediated by P2X7 receptor in RAW264.7 macrophages.
Abstract High glucose combined with high FFAs can contribute to the unfavorable development of type 2 diabetes mellitus (T2DM) and monocytes/macrophages are important in the occurrence and development of T2DM, which is regarded as a type of low‑grade inflammation. Although our previous study demonstrated that increased expression of P2X7 receptor (P2X7R) in peripheral blood monocytes may alter the innate immune system and that long non‑coding (lnc)RNA uc.48+ was involved in diabetic neuropathic pain, the involvement of uc.48+ mediated by the P2X7R in monocyte/macrophages during T2DM has not been reported. In t...
Source: International Journal of Molecular Medicine - May 9, 2018 Category: Molecular Biology Authors: Wu H, Wen F, Jiang M, Liu Q, Nie Y Tags: Int J Mol Med Source Type: research

Anti ‐angiogenic effects of CD73‐specific siRNA‐loaded nanoparticles in breast cancer‐bearing mice
Journal of Cellular Physiology, EarlyView.
Source: Journal of Cellular Physiology - May 9, 2018 Category: Cytology Authors: Ghasem Ghalamfarsa , Ali Rastegari , Fatemeh Atyabi , Hadi Hassannia , Mohammad Hojjat ‐Farsangi , Amir Ghanbari , Enayat Anvari , Jamshid Mohammadi , Gholamreza Azizi , Ali Masjedi , Mehdi Yousefi , Bahman Yousefi , Jamshid H Source Type: research

Role of Hypoxia-Inducible Factors 1 α (HIF1α) in SH-SY5Y Cell Autophagy Induced by Oxygen-Glucose Deprivation.
CONCLUSIONS Enhanced expression of HIF-1α may be related to autophagy activation in SH-SY5Y cells, thus contributing to ischemic/hypoxic brain damage. PMID: 29724989 [PubMed - in process]
Source: Medical Science Monitor - May 8, 2018 Category: Research Tags: Med Sci Monit Source Type: research