American Journal of Physiology. Cell Physiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Involvement of kinesins in skeletal dysplasia - a review
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00613.2023. Online ahead of print.ABSTRACTSkeletal dysplasias are group of rare genetic diseases resulting from mutations in genes encoding structural proteins of the cartilage extracellular matrix (ECM), signaling molecules, transcription factors, epigenetic modifiers, and several intracellular proteins. Cell division, organelle maintenance, and intracellular transport are all orchestrated by the cytoskeleton associated proteins, and intracellular processes effected through microtubule-associated movement are important for the function of skeletal cells. Amongst...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Roufaida Bouchenafa Francesca Manuela de Sousa Brito Katarzyna Anna Pirog Source Type: research

Effect of the Mitochondrial Transaminase (GOT2) on Membrane Potential Sensitive Respiration in Mitochondria of Differentiated C2C12 Muscle Cells
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00576.2023. Online ahead of print.ABSTRACTWe previously showed that the transaminase inhibitor, aminooxyacetic acid, reduced respiration energized at complex II (succinate dehydrogenase, SDH) in mitochondria isolated from mouse hindlimb muscle. The effect required a reduction in membrane potential with resultant accumulation of oxaloacetate (OAA), a potent inhibitor of SDH. To specifically assess the effect of the mitochondrial transaminase, glutamic oxaloacetic transaminase (GOT2) on complex II respiration and to determine the effect in intact cells as well as i...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Ritu Som Brian D Fink Liping Yu William I Sivitz Source Type: research

Microscopic computed tomography with AI-CNN powered image analysis: the path to phenotype the bleomycin-induced pulmonary injury
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00708.2023. Online ahead of print.ABSTRACTBleomycin (BLM)-induced lung injury in mice is a valuable model for investigating the molecular mechanisms that drive inflammation and fibrosis and for evaluating potential therapeutic approaches to treat the disease. Given high variability in the BLM model, it is critical to accurately phenotype the animals in the course of an experiment. In the current study, we aimed to demonstrate the utility of microscopic computed tomography (µCT) imaging combined with an artificial intelligence (AI) convolutional neural network (C...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Ingrid Henneke Christina Pilz Jochen Wilhelm Ioannis Alexopoulos Aysan Ezaddoustdar Regina Mukhametshina Norbert Weissmann Hossein Ardeschir Ghofrani Friedrich Grimminger Werner Seeger Ralph T Schermuly Malgorzata Wygrecka Baktybek Kojonazarov Source Type: research

Ubiquitin-driven G Protein-Coupled Receptor Inflammatory Signaling at the Endosome
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00161.2024. Online ahead of print.ABSTRACTG protein-coupled receptors (GPCRs) are ubiquitously expressed cell surface receptors that mediate numerous physiological responses and are highly druggable. Upon activation GPCRs rapidly couple to heterotrimeric G proteins and are then phosphorylated and internalized from the cell surface. Recent studies indicate that GPCRs not only localize at the plasma membrane but also exist in intracellular compartments where they are competent to signal. Intracellular signaling by GPCRs is best described to occur at endosomes. Seve...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Norton Cheng Julio M Pimentel JoAnn Trejo Source Type: research

SEMA3B inhibits TGF β-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00681.2023. Online ahead of print.ABSTRACTIdiopathic pulmonary fibrosis (IPF) is marked by the activation of fibroblasts, leading to excessive production and deposition of extracellular matrix (ECM) within the lung parenchyma. Despite the pivotal role of ECM overexpression in IPF, potential negative regulators of ECM production in fibroblasts have yet to be identified. Semaphorin class 3B (SEMA3B), a secreted protein highly expressed in lung tissues, has established roles in axonal guidance and tumor suppression. However, the role of SEMA3B in ECM production by f...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Dan Jk Yombo Sudhir Ghandikota Chanukya P Vemulapalli Priyanka Singh Anil G Jegga William D Hardie Sathish K Madala Source Type: research

Apelin-13: A Novel Approach to Suppressing Renin Production in RVHT
In this study, male Sprague-Dawley rats were divided into control, 2K1C model, and 2K1C with apelin-13 treatment groups. Renin expression was analyzed using immunohistochemistry and molecular techniques. Full-length (pro) renin receptor (fPRR) and soluble PRR (sPRR) levels were assessed via Western blotting, and cAMP levels were measured using ELISA. Plasma renin content, plasma renin activity (PRA), angiotensin II (Ang II), and sPRR levels were determined by ELISA. Human Calu-6 and mouse As4.1 cells were used to investigate renin production mechanisms. The 2K1C model exhibited increased systolic blood pressure (SBP), plas...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Ziqing Yan Teng Yang Xinxuan Li Zipeng Jiang Wankun Jia Jin Zhou Hui Fang Source Type: research

The effect of baseline O 2 conditions on the response of prostate cancer cells to hypoxia
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00155.2024. Online ahead of print.ABSTRACTThe transcriptional response to hypoxia is largely regulated by the hypoxia-inducible factors (HIFs), which induce the expression of genes involved in glycolysis, angiogenesis, proliferation, and migration. Virtually all cell culture-based hypoxia experiments have used near-atmospheric (18% O2) oxygen levels as baseline for comparison to hypoxia. However, this is hyperoxic compared to mammalian tissue microenvironments, where oxygen levels range from 2-9% O2 (physioxia). Thus, these experiments actually compare hyperoxia ...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Ricardo Alva Jacob E Wiebe Jeffrey A Stuart Source Type: research

Differential metabolic secretion between mdx mouse-derived spindle cell sarcomas and rhabdomyosarcomas drives tumor type development
In this study, we aimed to use metabolomics to better understand the rarity of SCS development in mdx mice. Gas chromatography-mass spectrometry was employed to compare the metabolic profiles of spontaneously developed SCS and RMS tumors from mdx mice, and metabolite supplementation assays and silencing experiments were used to assess the effects of metabolic differences in SCS tumor-derived cells. The levels of 75 metabolites exhibited differences between RMS and SCS, 25 of which were significantly altered. Further characterization revealed downregulation of non-essential amino acids, including alanine, in SCS tumors. Ala...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Emma Eko Tabe Niba Hiroyuki Awano Noriyuki Nishimura Hiroshi Koide Masafumi Matsuo Masakazu Shinohara Source Type: research

Long Noncoding RNA NEAT1 Enhances Phenotypic and Osteogenic Switching of Vascular Smooth Muscle Cells in Atherosclerosis via Scaffolding EZH2
This study provides new insights into the molecular mechanisms underlying the pathogenesis of AS and highlights the potential of NEAT1 as a therapeutic target of AS.PMID:38646788 | DOI:10.1152/ajpcell.00587.2023 (Source: American Journal of Physiology. Cell Physiology)
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Chengye Yin Zhuowang Ge Jiali Yuan Yuhan Chen Yong Tang Yin Xiang Yachen Zhang Source Type: research

Sodium-glucose co-transporter 1 promotes the bio-functions of perivascular preadipocytes mediated by Akt/mTOR/p70S6K signaling pathway
This study aimed to elucidate the role and mechanism of SGLT-1-mediated PVPACs bioactivity. PVPACs were cultured in vitro and applied ex vivo to the carotid arteries of mice using a lentivirus-based thermosensitive in situ gel (TISG). The groups were treated with Lv-SGLT1 (lentiviral vector, overexpression), Lv-siSGLT1 (RNA interference, knockdown), or specific signaling pathway inhibitors. Assays were conducted to assess changes in cell proliferation, apoptosis, glucose uptake, adipogenic differentiation, and vascular remodeling in the PVPACs. Protein expression was analyzed by western blotting, immunocytochemistry, and/o...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Zhiquan Liu Jiayu Wang Peiqing Tian Yixuan Liu Liyun Xing Caihua Fu Xianwei Huang Ping Liu Source Type: research

The electroneutral Na + -HCO 3 - cotransporter NBCn1 (SLC4A7) modulates colonic enterocyte pH i , proliferation and migration
Am J Physiol Cell Physiol. 2024 Apr 22. doi: 10.1152/ajpcell.00079.2024. Online ahead of print.ABSTRACTNBCn1 (SLC4A7) is one of the two major Na+-HCO3- cotransporters in the human colonic epithelium, expressed predominantly in the highly proliferating colonocytes at the cryptal base. Increased NBCn1 expression levels are reported in tumours, including colorectal cancer. The study explores its importance for maintenance of the intracellular pH (pHi),as well as the proliferative, adhesive, and migratory behavior of the self-differentiating Caco2bbe colonic tumour cell line. In the self-differentiating Caco2BBe cells, NBCn1 m...
Source: American Journal of Physiology. Cell Physiology - April 22, 2024 Category: Cytology Authors: Min Jiang Azam Salari Christian Stock Katerina Nikolovska Ebbe Boedtkjer Mahdi Amiri Ursula E Seidler Source Type: research

Decoding the Neurotoxic Effects of Propofol: Insights into the RAR α-Snhg1-Bdnf Regulatory Cascade
Am J Physiol Cell Physiol. 2024 Apr 15. doi: 10.1152/ajpcell.00547.2023. Online ahead of print.ABSTRACTThe potential neurotoxic effects of propofol, an extensively utilized anesthetic, underline the urgency to comprehend its influence on neuronal health. Insights into the role of the RARα-Snhg1-Bdnf network can offer significant advancements in minimizing these effects. The study targets the exploration of the RARα and Snhg1 regulatory network's influence on Bdnf expression in the realm of propofol-induced neurotoxicity. Harnessing the GEO database and utilizing JASPAR and RPISeq for projections, the study embarks on an ...
Source: American Journal of Physiology. Cell Physiology - April 15, 2024 Category: Cytology Authors: Yuhai Xu Xin Xin Tianzhu Tao Source Type: research

Macrophages in necrotic liver lesion repair: opportunities for therapeutical applications
Am J Physiol Cell Physiol. 2024 Apr 15. doi: 10.1152/ajpcell.00053.2024. Online ahead of print.ABSTRACTHealthy livers contain 80% of body resident macrophages known as Kupffer cells. In diseased livers, the number of Kupffer cells usually drops, but is compensated by infiltration of monocyte-derived macrophages, which can differentiate into Kupffer-like cells. Early studies suggest that Kupffer cells play important roles in both promoting liver injury as well as liver regeneration. Yet, the distinction of the functionalities from resident and infiltrating macrophages is not always made. By using more specific macrophage ma...
Source: American Journal of Physiology. Cell Physiology - April 15, 2024 Category: Cytology Authors: Yang Wang Robim M Rodrigues Cheng Chen Dechun Feng Luca Maccioni Bin Gao Source Type: research