GSE263844 Targeting metabolism to improve immunotherapy in GSNOR-deficient colorectal cancer
Contributors : Ana M Soldado ; Antonio R ArizaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensColorectal cancer (CRC) is the third most diagnosed type of cancer and the second leading cause of cancer death worldwide. Despite the increasing knowledge of CRC molecular biology and the development of new targeted therapies, its high heterogeneity hampers the efficacy of current treatments. Thus, there is a pressing need to identify new effective therapeutic targets and improve immune therapies for these patients. In this regard, S-nitrosoglutathione reductase (GSNOR) is a denitrosylase en...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE166507 CRISPR-based gene disruption coupled to targeted integration of novel, high-avidity, natural T cell receptors to WT1 for acute leukemia
Contributors : Eliana Ruggiero ; Ivan Merelli ; Chiara BoniniSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo better characterize and identify the candidate TCR among the receptors that we have isolated, we dissected the gene expression profiles and biological pathways of HD1- and HD3-TCR edited T cells by single cell sequencing. When challenged with target cells (either WT137-45- pulsed T2 cell line or primary AML blasts), results highlighted an immune response gene signature in each stimulated T cell culture, compared to resting counterparts. Interestingly, upregulation of gene...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE171982 Transcriptome sequencing of liver tissue in hepatocyte-specific IRF8-deficient mice (IRF8-cKO) mice and wild type (WT) mice that were subjected to warm ischemia/reperfusion(I/R) injury
Contributors : Wu Hongxi ; Shi GuangjiangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusInterferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is an essential lineage-specific transcriptional regulator in the IRF family, controls diverse cellular processes and is has been implicated in innate immunity, immune cell differentiation. IRF8 has been shown to have both oncogenic and tumor suppressor activities, in myeloid cells and nonhematopoietic cells. As a transcription factor, other functions which may contribute to its oncogenic and anti...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE203568 m6a methylation drives IMP1 regulation during human neuronal differentiation [RNA-seq]
Contributors : Marija Petri ć Howe ; Jasmine Harley ; Harry Crook ; Sofia E Serra ; Anob M Chakrabati ; Raphaëlle Luisier ; Rickie Patani ; Andres Ramos ; Pierre KleinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHuman neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet how these proteins precisely relate to neuronal development and coordinate the expression of functionall...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE203569 m6a methylation drives IMP1 regulation during human neuronal differentiation [iCLiP]
Contributors : Marija Petri ć Howe ; Jasmine Harley ; Harry Crook ; Sofia E Serra ; Anob M Chakrabati ; Raphaëlle Luisier ; Rickie Patani ; Andres Ramos ; Pierre KleinSeries Type : OtherOrganism : Homo sapiensHuman neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet how these proteins precisely relate to neuronal development and coordinate the expression of functionally coherent genes in a cell type specific mann...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research

GSE203570 m6a methylation drives IMP1 regulation during human neuronal differentiation [miCLIP]
Contributors : Marija Petri ć Howe ; Jasmine Harley ; Harry Crook ; Sofia E Serra ; Anob M Chakrabati ; Raphaëlle Luisier ; Rickie Patani ; Andres Ramos ; Pierre KleinSeries Type : Methylation profiling by high throughput sequencingOrganism : Homo sapiensHuman neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet how these proteins precisely relate to neuronal development and coordinate the expression of functional...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Homo sapiens Source Type: research

GSE203571 m6a methylation drives IMP1 regulation during human neuronal differentiation.
Series Type : Expression profiling by high throughput sequencing ; Other ; Methylation profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Methylation profiling by high throughput sequencing Homo sapiens Source Type: research

GSE228306 Wnt signaling regulates chemokine production and cell migration of circulating human monocytes
Contributors : Natalie Zelikson ; Shaina Ben ; Michal Caspi ; Raneen Tarabe ; Yonatan Shaleve ; Yael Peri-Paz Basson ; Oshrat Tayer-Shifman ; Elad Goldberg ; Shaye Kivity ; Rina Rosin-ArbesfeldSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe β-catenin dependent canonical Wnt signaling pathway plays a crucial role in maintaining normal homeostasis. However, when dysregulated, Wnt signaling is closely associated with various pathological conditions, including inflammation and different types of cancer.Here, we show a new connection between the leukocyte inflammatory respon...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE229982 Gene expression profiles at single cell level of lung epithelial cells from the Cracd WT and Cracd KO mice
This study reveals an unexpected role of CRACD tumor suppressor in restricting cell plasticity inducing cell de-differentiation, which provides new insights into NE cell plasticity of LUAD. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE243667 Increased AID Results in Mutations at the CRLF2 Locus Implicated in Latin American ALL Health Disparities
Contributors : Richard L Frock ; Valeria Rangel ; Jason N Sterrenberg ; Nicholas R PannunzioSeries Type : OtherOrganism : Homo sapiensActivation-induced cytidine deaminase (AID) is a B cell-specific base editor required during class switch recombination and somatic hypermutation for B cell maturation and antibody diversification. However, it has also been implicated as a factor in the etiology of several B cell malignancies. Evaluating the AID-induced mutation load in patients at-risk for certain types of blood cancers is critical in assessing disease severity and treatment options. Here, we have developed a digital PCR (...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research

GSE246304 A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli
This study provides compelling evidence that circRNAs can regulate biologically relevant functions in neurons, with modulatory effects at multiple levels of the phenotype, establishing a proof-of-principle for the regulatory role of circRNAs in neural processes. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE263462 Transcriptome comparison of monocytes, activated CD4 T cells, and activated CD8 T cells in healthy human peripheral blood
Contributors : Yafeng Wang ; Guiquan ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMolecular tether-mediated extracellular targeted protein degradation (ePTD) emerges as a promising drug modality. The existing strategies for ePTD have exploited several membrane degrader proteins. However, the current panel of ePTD degraders clearly needs to be expanded, as a given degrader may present tissue-dependent expression and activity. Based on screening of>50 receptors on endocytic rates and on their tissue distribution, we established a resource of potential endocytic carriers that m...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE263565 Single-Cell Transcriptomics Links Loss of Human Pancreatic β-Cell Identity to ER Stress
Contributors : Amadeo Mu ñoz Garcia ; Nathalie Groen ; Françoise Carlotti ; Eelco de KoningSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe maintenance of pancreatic islet architecture is crucial for proper β-cell function. We previously reported that disruption of human islet integrity could result in altered β-cell identity. Here we combine β-cell lineage tracing and single-cell transcriptomics to investigate the mechanisms underlying this process in primary human islet cells. Using drug-induced E R stress and cytoskeleton modification models, we demonstrate that alterin...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE263645 Whole transcriptome sequencing for wild type and IRAIN over-expression K562 cells
Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe development of tyrosine kinase inhibitors (TKIs) has revolutionarily increased the overall survival of patients with chronic myeloid leukemia (CML). However, drug resistance remains a major obstacle. Here, we demonstrated that a BCR-ABL1-independent long non-coding RNA, IRAIN, is constitutively expressed at low levels in CML, resulting in imatinib resistance. IRAIN knockdown decreased the sensitivity of CD34+ CML blasts and cell lines to imatinib, whereas IRAIN overexpression significantly increased sensitivity. Mechanistically, IRAI...
Source: GEO: Gene Expression Omnibus - April 13, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE263615 Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii
Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Toxoplasma gondiiWe tested the relevance of an established stem-cell-derived model of trophoblast development to Toxoplasma gondii infection. Human trophoblast stem cells were cultured under conditions suitable for cytotrophoblast cells or for the differentiation into syncytiotrophoblasts, and subsequently infected with T. gondii. RNA-seq data from both mock-treated and infected cells revealed differences between cell types and their respective responses to T. gondii infection. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 12, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Toxoplasma gondii Source Type: research