GSE264123 Transcriptional atlas of Daphnia magna
Contributors : Ishaan Dua ; Lev Y YampolskySeries Type : Expression profiling by high throughput sequencingOrganism : Daphnia magnaTranscriptomics studies are more likely to achieve predictive results when they rely on tissue- and cell-specific transcriptional data. Identification of cell types in novel model organisms by their transcriptional profiles is difficult without data on transcriptional differences among major tissues and anatomical features. Here we report the first dataset on tissue- and organ-specific transcriptomics in freshwater plankton crustacean Daphnia magna, reporting markers of eggs, hemocytes, gut, c...
Source: GEO: Gene Expression Omnibus - April 19, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Daphnia magna Source Type: research
GSE264138 A Novel Approach to Generate Enzyme-free Single Cell Suspensions from archived Tissues for miRNA Sequencing
Contributors : Stefan Scheuermann ; Sarah H ücker ; Annika Engel ; Nicole Ludwig ; Philipp Lebhardt ; Jens Langejürgen ; Stefan KirschSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensObtaining high-quality omics data at the single-cell level from archived human tissue samples is crucial for gaining insights into cellular heterogeneity and pushing the field of personalized medicine forward. In this technical brief we present a comprehensive methodological framework for the efficient enzyme-free preparation of tissue-derived single cell suspensions and their conversion into ...
Source: GEO: Gene Expression Omnibus - April 19, 2024 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research
GSE264267 GeoMX WGS of Mtb-infected mouse lesions
Contributors : Benjamin H Gern ; Andrew T Gustin ; Michael Gale Jr ; Michael Y Gerner ; Kevin B UrdahlSeries Type : OtherOrganism : Mus musculusTo gain holistic of the immune landscape of lesions following infection with Mycobacterium tuberculosis, we aerosol infected C3HeB/FeJ mice with 1-3 CFU of Mtb (SA161 strain). 35 days following infection, lungs were harvested and fixed, then embedded and frozed in OCT. 10um sections were obtained, classified as necrotic or non-necrotic by visual inspection and brightfield imaging, then stained with markers delineating lesions, and subsequently processed and sampled using the nanos...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Other Mus musculus Source Type: research
GSE149000 Sequencing of AsPC-1 cells with CYP3A5 expression modulation
Contributors : Ming Shao ; William C Wright ; Taosheng ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: To understand the transcriptome-wide effect of CYP3A5 knockdown or over-expression in AsPC-1 cells (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE158626 Targeting Set7 in an experimental model of diabetic nephropathy
Contributors : Jun Okabe ; Scott Maxwell ; Assam El-OstaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusDiabetic nephropathy (DN) is a leading cause of renal failure with limited therapeutic options. The Set7 methyltransferase regulates pathways involved in diabetes, however, its role in DN is poorly understood. Using a genetic model of DN we show improved urinary albumin excretion and glomerular pathology in Set7KO mice. Single cell RNA-seq (scRNA-seq) identified proximal tubule (PTC), glomerular endothelial (GEN), mesenchymal (MC) and podocytes (PDC) as the major c...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Mus musculus Source Type: research
