GSE266025 The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision
Contributor : jiayin gaoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe first cell fate decision is the process by which cells of an embryo take on distinct lineage identities for the first time, thus representing the beginning of developmental patterning. Here, we demonstrate that the molecular chaperone heat shock protein A2 (HSPA2), a member of the 70 kDa heat shock protein (HSP70) family, is asymmetrically expressed in the late 2-cell stage of mouse embryos. The knockdown of Hspa2 in one of the two-cell blastomeres prevented its progeny predominantly toward the inner cell mas...
Source: GEO: Gene Expression Omnibus - May 14, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE267128 Next Generation Sequencing of the transcriptomes of prostate cancer cells with or without ectopic expression of miR-5100
Contributor : An ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGene expression profilings for prostate cancer cells after miR-5100 overexpression (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - May 14, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE267162 A multi-adjuvant nanovaccine platform based on targeted delivery of specific antigens for cancer immunotherapy
Contributors : Wen Li ; Qiu-Ling ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCance vaccines have become a milestone in immunotherapy, but inadequate activation rate of antigen presenting cells (APCs) and low delivery efficiency of specific antigen have widely limited their clinical application. Here we design an engineered vaccine platform based on targeted delivery of specific antigens to activated APCs. This vaccine platform is implemented by loading stimulator of interferon genes agonist and tumor lysate protein with calcium phosphate as adjuvants, and coating the surfac...
Source: GEO: Gene Expression Omnibus - May 14, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE267207 FBXO5 degrades DOK6 to enhance cell mechanics and sensitivity to Temozolomide
Contributors : Chao Wang ; Mengqi WangSeries Type : OtherOrganism : Homo sapiensstable CRISPR/Cas9 sgRNA-expression U251 cells were treated with 200nM or 1500nM temozolomide for 72 hours, and then, we screened temozolomide-resistance genes by negative selection and temozolomide-sensitive genes by positive selection (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - May 14, 2024 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research

GSE267303 Developmentally regulated alternate 3 ’ end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage
Contributors : Berry Cameron ; Fuller MargaretSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogasterTo determine the extent of alternative 3' end cleavage during Drosophila spermatogenesis, we performed 3' end sequencing of Drosophila testes from flies at different time points post heat shock (pHS) that were mutant for bam and also had a heat shock inducible transgene expressing Bam.We then performed Polysome profiling of testes at 24, 48 and 72 hours PHS and created 3' end sequencing libraries from the free, 40S, 60s, 80S, 2-3 ribosomes, and 4+ ribosomes fractions for each dat...
Source: GEO: Gene Expression Omnibus - May 14, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila melanogaster Source Type: research