Chemical and activity investigation on metabolites from the endophytic fungus < em > Penicillium macrosclerotiorum < /em > isolated from < em > Ilex pubescens Hook. et Arn < /em >
Nat Prod Res. 2024 Apr 22:1-5. doi: 10.1080/14786419.2024.2337111. Online ahead of print.ABSTRACTPenicillium macrosclerotiorum was isolated for the first time from the leaves of Ilex pubescens Hook. et Arn (VSN-2022-03-18-001) and found to produce bioactive compounds. Seven compounds were obtained from the fermentation broth and mycelia of P. macrosclerotiorum and identified as palmitic acid (1), methyl 6-acetyl-5,7,8-trihydroxy-4-methoxy-2-naphthoate (2), ergosterol (3), daidzein (4), oleuropein (5), pedunculoside (6) and acteoside (7). Compounds 3, 4 and 7 exhibited antioxidant activity against diphenyl picryl hydrazinyl...
Source: Natural Product Research - April 22, 2024 Category: Biochemistry Authors: Jianshu Chen Zhaoyang Tao Shengli Yang Source Type: research
A Dual-mode platform for the rapid detection of Escherichia coli O157:H7 based on CRISPR/Cas12a and RPA
In conclusion, the established platform showed excellent specificity and strong universality. Modifying the target gene and its primers can broaden the platform's applicability to detect various other foodborne pathogens.PMID:38647692 | DOI:10.1007/s00216-024-05301-0 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 22, 2024 Category: Chemistry Authors: Jiawei Luo Danhong Xu Jinbin Wang Hua Liu You Li Yan Zhang Haijuan Zeng Bo Deng Xiaofeng Liu Source Type: research
A Dual-mode platform for the rapid detection of Escherichia coli O157:H7 based on CRISPR/Cas12a and RPA
In conclusion, the established platform showed excellent specificity and strong universality. Modifying the target gene and its primers can broaden the platform's applicability to detect various other foodborne pathogens.PMID:38647692 | DOI:10.1007/s00216-024-05301-0 (Source: Anal Sci)
Source: Anal Sci - April 22, 2024 Category: Chemistry Authors: Jiawei Luo Danhong Xu Jinbin Wang Hua Liu You Li Yan Zhang Haijuan Zeng Bo Deng Xiaofeng Liu Source Type: research
Evaluating smartphone-based optical readouts for immunoassays in human and veterinary healthcare: A comparative study
This study uses high-resolution cameras in mobile devices to capture and process bioassay images, enabling the quantification of diverse biomarkers across a range of diagnostic tests conducted on 96-well microplates. The study evaluates the effectiveness of this technology through protein quantification techniques and immunoassays that generate colorimetric responses at specific wavelengths. It includes the assessment of bicinchoninic acid and Bradford protein quantification methods, alongside a conventional immunoassay for detecting mare antibodies in colostrum to monitor foal immunodeficiencies. Further application invol...
Source: Talanta - April 22, 2024 Category: Chemistry Authors: Melania Mesas G ómez Esther Juli án Lara Armengou Maria Isabel Pividori Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Mitigation of biofouling in membrane bioreactors by quorum-quenching bacteria during the treatment of metal-containing wastewater
Environ Sci Pollut Res Int. 2024 Apr 22. doi: 10.1007/s11356-024-33336-4. Online ahead of print.ABSTRACTQuorum quenching (QQ) is an efficient way to mitigate membrane biofouling in a membrane bioreactor (MBR) during wastewater treatment. A QQ bacterium, Lysinibacillus sp. A4, was isolated and used to mitigate biofouling in an MBR during the treatment of wastewater containing metals. A QQ enzyme (named AilY) was cloned from A4 and identified as a metallo-β-lactamase-like lactonase. The QQ activity of A4 and that of Escherichia coli BL21 (DE3) overexpressing AilY could be promoted by Fe2+, Mn2+, and Zn2+ while remaining una...
Source: Environmental Science and Pollution Research International - April 22, 2024 Category: Environmental Health Authors: Shimei Ge Huiting Yang Yaru Li Xiaohui Chen Ruixue Yang Xinjiao Dong Source Type: research
Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a
Nucleic Acids Res. 2024 Apr 22:gkae283. doi: 10.1093/nar/gkae283. Online ahead of print.ABSTRACTCRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use RNA guides to find and cleave target DNA sites, the two enzymes differ in terms of protospacer-adjacent motif (PAM) requirements, guide architecture and cleavage mechanism. In the last years, rational engineering led to the creation of PAM-relaxed variants SpRYCas9 and impLbCas12a to broaden the targetable DNA space. By employing their catalytically inactive varia...
Source: Cell Research - April 22, 2024 Category: Cytology Authors: Lorenzo Olivi Cleo Bagchus Victor Pool Ezra Bekkering Konstantin Speckner Hidde Offerhaus Wen Y Wu Martin Depken Koen J A Martens Raymond H J Staals Johannes Hohlbein Source Type: research
Proteomic profiling of antibiotic ‐resistant Escherichia coli GW‐AmxH19 isolated from hospital wastewater treated with physical plasma
AbstractMicroorganisms which are resistant to antibiotics are a global threat to the health of humans and animals. Wastewater treatment plants are known hotspots for the dissemination of antibiotic resistances. Therefore, novel methods for the inactivation of pathogens, and in particular antibiotic-resistant microorganisms (ARM), are of increasing interest. An especially promising method could be a water treatment by physical plasma which provides charged particles, electric fields, UV-radiation, and reactive species. The latter are foremost responsible for the antimicrobial properties of plasma. Thus, with plasma it might...
Source: Proteomics - April 22, 2024 Category: Biochemistry Authors: Veronika Hahn,
Daniela Z ühlke,
Hauke Winter,
Annchristin Landskron,
Jörg Bernhardt,
Susanne Sievers,
Michael Schmidt,
Thomas von Woedtke,
Katharina Riedel,
Juergen F. Kolb Tags: RESEARCH ARTICLE Source Type: research
GSE245749 TnpB homologs exapted from transposons are RNA-guided transcription factors
Contributors : Tanner Wiegand ; Florian T Hoffmann ; Matthew W Walker ; Stephen Tang ; Egill Richard ; Hoang C Le ; Chance Meers ; Samuel H SternbergSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencing ; OtherOrganism : Enterobacter cloacae ; Enterobacter sp. BIDMC93 ; Escherichia coliTransposon-encoded tnpB and iscB genes encode RNA-guided DNA nucleases that promote their own selfish spread through targeted DNA cleavage and homologous recombination. These widespread gene families were repeatedly domesticated over evolutionary timescales, le...
Source: GEO: Gene Expression Omnibus - April 22, 2024 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencing Other Enterobacter cloacae Enterobacter sp. BIDMC93 Escherichia coli Source Type: research
Mitigating antimicrobial resistance, an approach to stewardship in canine urinary tract infection
AbstractUrinary tract infection (UTI) caused by antimicrobial resistant bacteria is common in dogs leading to serious health impact in pet animal as well as on human health. Understanding the prevalent uropathogens and their drug susceptibility is essential for limiting the antimicrobial resistance through implementation of stewardship policies. In view of this, present study was envisaged to determine the prevalent bacterial uropathogens and their antibiogram from clinical cases of canine UTI. Urine samples were collected from 35 dogs presented with clinical signs of UTI and a total of 27 bacterial isolates were recovered...
Source: Veterinary Research Communications - April 22, 2024 Category: Veterinary Research Source Type: research
CX-6258 hydrochloride hydrate: A potential non-nucleoside inhibitor targeting the RNA-dependent RNA polymerase of norovirus
Virology. 2024 Apr 17;595:110088. doi: 10.1016/j.virol.2024.110088. Online ahead of print.ABSTRACTHuman norovirus (HuNoV), a primary cause of non-bacterial gastroenteritis, currently lacks approved treatment. RdRp is vital for virus replication, making it an attractive target for therapeutic intervention. By application of structure-based virtual screening procedure, we present CX-6258 hydrochloride hydrate as a potent RdRp non-nucleoside inhibitor, effectively inhibiting HuNoV RdRp activity with an IC50 of 3.61 μM. Importantly, this compound inhibits viral replication in cell culture, with an EC50 of 0.88 μM. In vitro b...
Source: Virology - April 21, 2024 Category: Virology Authors: Yang Liu Quanjie Li Huihan Shao Yang Mao Lufei Liu Dongrong Yi Zhaojun Duan Huiqing Lv Shan Cen Source Type: research
Poly (vinyl alcohol)/sodium alginate/carboxymethyl chitosan multifunctional hydrogel loading HKUST-1 nanoenzymes for diabetic wound healing
In this study, a copper-based MOF (TAX@HKUST-1) highly loaded with taxifolin (TAX) with a drug loading of 41.94 ± 2.60 % was prepared. In addition, it has excellent catalase activity, and by constructing an oxygen-releasing hydrogel (PTH) system with calcium peroxide (CaO2), it can be used as a nano-enzyme to promote the generation of oxygen from hydrogen peroxide (H2O2) to provide sufficient oxygen to the wound, and at the same time, solve the problem of the oxidative stress damage caused by excess H2O2 to the cells during the oxygen-releasing process. On the other hand, TAX and HKUST-1 in PTH synergistically promoted an...
Source: International Journal of Biological Macromolecules - April 21, 2024 Category: Biochemistry Authors: Guodong Chai Ning Wang Meiling Xu Lina Ma Xinglong Liu Qiteng Ding Shuai Zhang Anning Li Guofeng Xia Yingchun Zhao Wencong Liu Dadong Liang Chuanbo Ding Source Type: research
Molecules, Vol. 29, Pages 1888: Dragon & rsquo;s Blood-Loaded Mesoporous Silica Nanoparticles for Rapid Hemostasis and Antibacterial Activity
In this study, different amounts of DB were loaded on mesoporous silica nanoparticles (MSNs) to prepare a series of DB-MSN composites (5DB-MSN, 10DB-MSN, and 20DB-MSN). DB-MSN could quickly release DB and activate the intrinsic blood coagulation cascade simultaneously by DB and MSN. Hemostasis tests demonstrated that DB-MSN showed superior hemostatic effects than either DB or MSNs alone, and 10DB-MSN exhibited the best hemostatic effect. In addition, the antibacterial activities of DB-MSN against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) improved with the increase in DB. Furthermore, the hemolysis as...
Source: Molecules - April 21, 2024 Category: Chemistry Authors: Cuiyun Yin Yihang Li Jing Yu Zhaoyou Deng Shifang Liu Xuanchao Shi Deying Tang Xi Chen Lixia Zhang Tags: Article Source Type: research
Viruses, Vol. 16, Pages 645: Lysis Physiology of Pseudomonas aeruginosa Infected with ssRNA Phage PRR1
ord
The phage PRR1 belongs to the Leviviridae family, a group of ssRNA bacteriophages that infect Gram-negative bacteria. The variety of host cells is determined by the specificity of PRR1 to a pilus encoded by a broad host range of IncP-type plasmids that confer multiple types of antibiotic resistance to the host. Using P. aeruginosa strain PAO1 as a host, we analyzed the PRR1 infection cycle, focusing on cell lysis. PRR1 infection renders P. aeruginosa cells sensitive to lysozyme approximately 20 min before the start of a drop in suspension turbidity. At the same time, infected cells start to accumulate lipophilic an...
Source: Viruses - April 21, 2024 Category: Virology Authors: Rimantas Daugelavi čius Greta Daujotait ė Dennis H. Bamford Tags: Article Source Type: research
