Clathriketal, a new tricyclic spiroketal compound from marine sponge < em > Clathria prolifera < /em > attenuates serine exopeptidase dipeptidyl peptidase-IV
Nat Prod Res. 2021 Jul 27:1-9. doi: 10.1080/14786419.2021.1956491. Online ahead of print.ABSTRACTAn undescribed tricyclic spiroketal compound clathriketal was purified from the solvent extract of the Microcionidae sponge Clathria prolifera, and was characterised as 7-(hydroxymethyl)-13-methoxy-3,11-dimethyl-4-oxo-octahydrospiro[chromene-9,13-pyran]-11-yl propionate by spectroscopic experiments. Clathriketal exhibited significant anti-hyperglycemic property by attenuating serine protease dipeptidyl peptidase-IV (IC50 0.37 mM), and its activity was comparable with the standard diprotin A (IC50 0.31 mM). The spiroketal also e...
Source: Natural Product Research - July 28, 2021 Category: Biochemistry Authors: Prima Francis Kajal Chakraborty Source Type: research

New cytochalasans from an endophytic < em > Xylaria < /em > species associated with Costa Rican < em > Palicourea elata < /em > (Rubiaceae)
Nat Prod Res. 2021 Jul 27:1-8. doi: 10.1080/14786419.2021.1956490. Online ahead of print.ABSTRACTFour new leucine-derived cytochalasans, possessing a 5,6,5,8-ring (1) and a 5,6,11-ring core (2-4), were isolated from a cultivated endophytic fungus Xylaria sp. strain WH2D4 (Xylariaceae). This fungus was isolated from leaves of the neotropical tree species Palicourea elata (Sw.) Borhidi (Rubiaceae) collected in Costa Rica. The chemical structures were determined by employing IR, MS as well as 1D- and 2D-NMR experiments. The stereochemistry at C-15 of compound 4 was determined by quantum calculations. The isolated compounds di...
Source: Natural Product Research - July 27, 2021 Category: Biochemistry Authors: Wolfgang Hinterdobler Markus Bacher Bao-Bao Shi Dieter Baurecht Irmgard Krisai-Greilhuber Monika Schmoll Lothar Brecker Karin Valant-Vetschera Johann Schinnerl Source Type: research

Natural multi-target modulators of pathological pathways in Alzheimer's disease isolated from the < em > Rhinella arenarum < /em > skin
Nat Prod Res. 2021 Jul 27:1-5. doi: 10.1080/14786419.2021.1953022. Online ahead of print.ABSTRACTThe skin of anuran amphibians is a rich source of compounds with great medicinal potential. Alzheimer's disease (AD) is a complex disease associated with numerous pathological pathways, making their simultaneous modulation necessary. Nowadays the development of anti-AD drugs is focused on a Multi-Target Directed Ligands strategy. Herein we report the bioactivity of the skin extracts of the toad Rhinella arenarum obtained by an invasive and non-invasive methods, against five AD pathological targets (AChE, BChE, MAO-B, antioxidan...
Source: Natural Product Research - July 27, 2021 Category: Biochemistry Authors: Roque Spinelli Álvaro Rietmann Ivan Sanchis Álvaro Sebastián Siano Source Type: research

Sulfation of chitosan from < em > Sepia kobiensis < /em > as potential anticoagulant and antibacterial molecule
Nat Prod Res. 2021 Jul 24:1-7. doi: 10.1080/14786419.2021.1956492. Online ahead of print.ABSTRACTThe present work aimed to synthesis of chitin, chitosan and sulfation of chitosan from cuttlebone of cuttlefish Sepia kobiensis. Principally chitin was extracted through sequential processes of demineralisation and deproteinzation. Then chitosan was synthesized by a deacetylation and finally sulfated at semi-heterogeneous condition using chlorosulfonic acid in N,N-dimethylformamide. The synthesized macromolecules were characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM an...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Pasiyappazham Ramasamy Sivasankari Sekar Sivagurunathan Paramasivam Priyatharsini Suri Uma Chinnaiyan Rajesh Singh Bharath Prasad Tanguturi Raghavaiah Vidya Devanathadesikan Seshadri Source Type: research

A comprehensive insight on the biological potential of embelin and its derivatives
Nat Prod Res. 2021 Jul 26:1-15. doi: 10.1080/14786419.2021.1955361. Online ahead of print.ABSTRACTNaturally occurring bioactive molecules are known for their diverse biological applications such as antimicrobial, anticancer, anti-inflammatory, and analgesic activities. Also, some of the natural products act as medicinal drugs. Further, bioactive cell-permeable molecule embelin has been reported for its diverse biological activities such as antimalarial, anticancer, and anti-inflammatory in the literature. With the continuation of our research work on biologically active molecules, based on structural activity relationship ...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: N Jeelan Basha S M Basavarajaiah Swathi Baskaran Prasanna Kumar Source Type: research

Antibacterial and antioxidant potential of the phenolic extract and its fractions isolated from < em > Allium ascalonicum < /em > (onion) peel
Nat Prod Res. 2021 Jul 24:1-5. doi: 10.1080/14786419.2021.1948040. Online ahead of print.ABSTRACTAqueous methanolic (20:80) crude extract of the outer peel of Allium ascalonicum (onions) was fractionated into anthocyanin (II), acidic (i.e., phenolic acids, Ia), neutral A (i.e., flavanols, Ib) and neutral B (i.e., flavonols, Ic) phenols using C-18 and hydrophilic-lipophilic balanced (HLB) Co-polymer cartridges. The fractions were analysed for total phenolic, total flavonoids, antibacterial activity, antioxidant potential and lipoxygenase (LOX) inhibition. The recovery of total phenols and bio functional activity in all frac...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Lubna Mobin Mohammad Abdul Haq Rashida Ali Shahina Naz Syed Ghufran Saeed Source Type: research

Cadinane-type sesquiterpenes with potential anti-inflammatory and anti-HIV activities from the stems and leaves of < em > Mappianthus iodoides < /em >
Nat Prod Res. 2021 Jul 24:1-7. doi: 10.1080/14786419.2021.1956921. Online ahead of print.ABSTRACTEight cadinane-type sesquiterpenes, including a new cadinane-type sesquiterpene, named as mappianiodene (1), and seven known analogues (2-8), were isolated and identified from the stems and leaves of Mappianthus iodoides. The chemical structure and absolute configurations of 1 was elucidated by extensive spectral methods and the known compounds were identified by comparing their experimental spectral data with the reported spectral data in the literature. The anti-inflammatory and anti-HIV activities of those isolated cadinane-...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Yan-Ping Liu Xiao-Mei Yu Ze-Hua Qiao Bo Jiang Lan Xie Hao-Xuan Tang Xiao Liang Yan-Hui Fu Source Type: research

Sulfation of chitosan from < em > Sepia kobiensis < /em > as potential anticoagulant and antibacterial molecule
Nat Prod Res. 2021 Jul 24:1-7. doi: 10.1080/14786419.2021.1956492. Online ahead of print.ABSTRACTThe present work aimed to synthesis of chitin, chitosan and sulfation of chitosan from cuttlebone of cuttlefish Sepia kobiensis. Principally chitin was extracted through sequential processes of demineralisation and deproteinzation. Then chitosan was synthesized by a deacetylation and finally sulfated at semi-heterogeneous condition using chlorosulfonic acid in N,N-dimethylformamide. The synthesized macromolecules were characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM an...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Pasiyappazham Ramasamy Sivasankari Sekar Sivagurunathan Paramasivam Priyatharsini Suri Uma Chinnaiyan Rajesh Singh Bharath Prasad Tanguturi Raghavaiah Vidya Devanathadesikan Seshadri Source Type: research

A comprehensive insight on the biological potential of embelin and its derivatives
Nat Prod Res. 2021 Jul 26:1-15. doi: 10.1080/14786419.2021.1955361. Online ahead of print.ABSTRACTNaturally occurring bioactive molecules are known for their diverse biological applications such as antimicrobial, anticancer, anti-inflammatory, and analgesic activities. Also, some of the natural products act as medicinal drugs. Further, bioactive cell-permeable molecule embelin has been reported for its diverse biological activities such as antimalarial, anticancer, and anti-inflammatory in the literature. With the continuation of our research work on biologically active molecules, based on structural activity relationship ...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: N Jeelan Basha S M Basavarajaiah Swathi Baskaran Prasanna Kumar Source Type: research

Antibacterial and antioxidant potential of the phenolic extract and its fractions isolated from < em > Allium ascalonicum < /em > (onion) peel
Nat Prod Res. 2021 Jul 24:1-5. doi: 10.1080/14786419.2021.1948040. Online ahead of print.ABSTRACTAqueous methanolic (20:80) crude extract of the outer peel of Allium ascalonicum (onions) was fractionated into anthocyanin (II), acidic (i.e., phenolic acids, Ia), neutral A (i.e., flavanols, Ib) and neutral B (i.e., flavonols, Ic) phenols using C-18 and hydrophilic-lipophilic balanced (HLB) Co-polymer cartridges. The fractions were analysed for total phenolic, total flavonoids, antibacterial activity, antioxidant potential and lipoxygenase (LOX) inhibition. The recovery of total phenols and bio functional activity in all frac...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Lubna Mobin Mohammad Abdul Haq Rashida Ali Shahina Naz Syed Ghufran Saeed Source Type: research

Cadinane-type sesquiterpenes with potential anti-inflammatory and anti-HIV activities from the stems and leaves of < em > Mappianthus iodoides < /em >
Nat Prod Res. 2021 Jul 24:1-7. doi: 10.1080/14786419.2021.1956921. Online ahead of print.ABSTRACTEight cadinane-type sesquiterpenes, including a new cadinane-type sesquiterpene, named as mappianiodene (1), and seven known analogues (2-8), were isolated and identified from the stems and leaves of Mappianthus iodoides. The chemical structure and absolute configurations of 1 was elucidated by extensive spectral methods and the known compounds were identified by comparing their experimental spectral data with the reported spectral data in the literature. The anti-inflammatory and anti-HIV activities of those isolated cadinane-...
Source: Natural Product Research - July 26, 2021 Category: Biochemistry Authors: Yan-Ping Liu Xiao-Mei Yu Ze-Hua Qiao Bo Jiang Lan Xie Hao-Xuan Tang Xiao Liang Yan-Hui Fu Source Type: research

Antibacterial secondary metabolites from < em > Vernonia auriculifera < /em > Hiern (Asteraceae) against MDR phenotypes
Nat Prod Res. 2021 Jul 23:1-4. doi: 10.1080/14786419.2021.1953024. Online ahead of print.ABSTRACTPurification of the aerial parts of Vernonia auriculifera Hiern afforded steroids (1-2), flavonoids (3-5), and polyalcohol (6). Their structures were determined using spectral evidences as well as by comparison with reported data. Iodonitrotetrazolium chloride (INT) colorimetric assay was used to assess the antibacterial activity of the extract and isolates against 13 pathogenic strains. The crude extract showed strong antibacterial activity (MIC
Source: Natural Product Research - July 23, 2021 Category: Biochemistry Authors: Clara Jepkoech Leonidah Kerubo Omosa Vaderament-A Nchiozem-Ngnitedem Evans O Kenanda Michel-Gael F Guefack Armelle T Mbaveng Victor Kuete Matthias Heydenreich Source Type: research

Antibacterial secondary metabolites from < em > Vernonia auriculifera < /em > Hiern (Asteraceae) against MDR phenotypes
Nat Prod Res. 2021 Jul 23:1-4. doi: 10.1080/14786419.2021.1953024. Online ahead of print.ABSTRACTPurification of the aerial parts of Vernonia auriculifera Hiern afforded steroids (1-2), flavonoids (3-5), and polyalcohol (6). Their structures were determined using spectral evidences as well as by comparison with reported data. Iodonitrotetrazolium chloride (INT) colorimetric assay was used to assess the antibacterial activity of the extract and isolates against 13 pathogenic strains. The crude extract showed strong antibacterial activity (MIC
Source: Natural Product Research - July 23, 2021 Category: Biochemistry Authors: Clara Jepkoech Leonidah Kerubo Omosa Vaderament-A Nchiozem-Ngnitedem Evans O Kenanda Michel-Gael F Guefack Armelle T Mbaveng Victor Kuete Matthias Heydenreich Source Type: research

Antibacterial secondary metabolites from < em > Vernonia auriculifera < /em > Hiern (Asteraceae) against MDR phenotypes
Nat Prod Res. 2021 Jul 23:1-4. doi: 10.1080/14786419.2021.1953024. Online ahead of print.ABSTRACTPurification of the aerial parts of Vernonia auriculifera Hiern afforded steroids (1-2), flavonoids (3-5), and polyalcohol (6). Their structures were determined using spectral evidences as well as by comparison with reported data. Iodonitrotetrazolium chloride (INT) colorimetric assay was used to assess the antibacterial activity of the extract and isolates against 13 pathogenic strains. The crude extract showed strong antibacterial activity (MIC
Source: Natural Product Research - July 23, 2021 Category: Biochemistry Authors: Clara Jepkoech Leonidah Kerubo Omosa Vaderament-A Nchiozem-Ngnitedem Evans O Kenanda Michel-Gael F Guefack Armelle T Mbaveng Victor Kuete Matthias Heydenreich Source Type: research

Cytotoxic abietane-type diterpenoids from roots of < em > Salvia spinosa < /em > and their < em > in Silico < /em > pharmacophore modeling
Nat Prod Res. 2021 Jul 22:1-6. doi: 10.1080/14786419.2021.1952202. Online ahead of print.ABSTRACTThe roots of Salvia spinosa L. (Lamiaceae) were extracted with hexane, dichloromethane (DCM) and ethyl acetate. The DCM extract exhibited cytotoxic activity (IC50 32.7 µg/mL) against MFC-7 breast cancer cell line in MTT colorimetric bioassay. Ferruginol (1), taxodione (2), 12-deoxy-6-hydroxy-6,7-dehydroroyleanone (3), 14-deoxycoleon U (4), 15-deoxyfuerstione (5) and taxodone (6) were isolated from the DCM roots extract. Their structures were elucidated by a combination of spectroscopic analyses including EIMS and 1H- and ...
Source: Natural Product Research - July 22, 2021 Category: Biochemistry Authors: Amir Reza Jassbi Hossein Hadavand Mirzaei Omidreza Firuzi Somayeh Pirhadi Mojtaba Asadollahi Jima N Chandran Bernd Schneider Source Type: research

Cytotoxic abietane-type diterpenoids from roots of < em > Salvia spinosa < /em > and their < em > in Silico < /em > pharmacophore modeling
Nat Prod Res. 2021 Jul 22:1-6. doi: 10.1080/14786419.2021.1952202. Online ahead of print.ABSTRACTThe roots of Salvia spinosa L. (Lamiaceae) were extracted with hexane, dichloromethane (DCM) and ethyl acetate. The DCM extract exhibited cytotoxic activity (IC50 32.7 µg/mL) against MFC-7 breast cancer cell line in MTT colorimetric bioassay. Ferruginol (1), taxodione (2), 12-deoxy-6-hydroxy-6,7-dehydroroyleanone (3), 14-deoxycoleon U (4), 15-deoxyfuerstione (5) and taxodone (6) were isolated from the DCM roots extract. Their structures were elucidated by a combination of spectroscopic analyses including EIMS and 1H- and ...
Source: Natural Product Research - July 22, 2021 Category: Biochemistry Authors: Amir Reza Jassbi Hossein Hadavand Mirzaei Omidreza Firuzi Somayeh Pirhadi Mojtaba Asadollahi Jima N Chandran Bernd Schneider Source Type: research

Characterization of chemical constituents with their antioxidant and anti-melanogenesis activities from the roots of < em > Ampelopsis japonica < /em >
Nat Prod Res. 2021 Jul 18:1-5. doi: 10.1080/14786419.2021.1939335. Online ahead of print.ABSTRACTThree glycosylated stilbenes (1-3), two anthraquinones (4, 5), one lignan (6), five tannins (7-11), two amino acids (12, 13), and one auronol (14) were isolated from the root of Ampelopsis japonica. All compounds, except for 4, 6, and 11 were obtained from this species for the first time. Compounds 6-9 could notably inhibit ROS generations in HaCaT keratinocyte cells with IC50 values of 5.28, 4.83, 0.87, and 1.66 μM, respectively. Compounds 8-10 showed potent DPPH free radical scavenging effects with IC50 values of 14.37, 16...
Source: Natural Product Research - July 19, 2021 Category: Biochemistry Authors: Chia-Lin Lee Yun-Lian Jhan Hsiu-Mei Chiang Yi-Hung Chen Chao-Jung Chen Yuan-Shiun Chang Source Type: research

Characterization of chemical constituents with their antioxidant and anti-melanogenesis activities from the roots of < em > Ampelopsis japonica < /em >
Nat Prod Res. 2021 Jul 18:1-5. doi: 10.1080/14786419.2021.1939335. Online ahead of print.ABSTRACTThree glycosylated stilbenes (1-3), two anthraquinones (4, 5), one lignan (6), five tannins (7-11), two amino acids (12, 13), and one auronol (14) were isolated from the root of Ampelopsis japonica. All compounds, except for 4, 6, and 11 were obtained from this species for the first time. Compounds 6-9 could notably inhibit ROS generations in HaCaT keratinocyte cells with IC50 values of 5.28, 4.83, 0.87, and 1.66 μM, respectively. Compounds 8-10 showed potent DPPH free radical scavenging effects with IC50 values of 14.37, 16...
Source: Natural Product Research - July 19, 2021 Category: Biochemistry Authors: Chia-Lin Lee Yun-Lian Jhan Hsiu-Mei Chiang Yi-Hung Chen Chao-Jung Chen Yuan-Shiun Chang Source Type: research

Characterization of chemical constituents with their antioxidant and anti-melanogenesis activities from the roots of < em > Ampelopsis japonica < /em >
Nat Prod Res. 2021 Jul 18:1-5. doi: 10.1080/14786419.2021.1939335. Online ahead of print.ABSTRACTThree glycosylated stilbenes (1-3), two anthraquinones (4, 5), one lignan (6), five tannins (7-11), two amino acids (12, 13), and one auronol (14) were isolated from the root of Ampelopsis japonica. All compounds, except for 4, 6, and 11 were obtained from this species for the first time. Compounds 6-9 could notably inhibit ROS generations in HaCaT keratinocyte cells with IC50 values of 5.28, 4.83, 0.87, and 1.66 μM, respectively. Compounds 8-10 showed potent DPPH free radical scavenging effects with IC50 values of 14.37, 16...
Source: Natural Product Research - July 19, 2021 Category: Biochemistry Authors: Chia-Lin Lee Yun-Lian Jhan Hsiu-Mei Chiang Yi-Hung Chen Chao-Jung Chen Yuan-Shiun Chang Source Type: research

A blue enzyme from marine bacterium for green technological applications
Nat Prod Res. 2021 Jul 13:1-2. doi: 10.1080/14786419.2021.1948044. Online ahead of print.ABSTRACTLaccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular laccases as compared to fungal laccases. Therefore, there are many advantages of bacterial laccases over fungal laccases.PMID:34254841 | DOI:10.1080/14786419.2021.1948044 (Source: Natu...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Abraham P Karlapudi Vidya P Kodali Source Type: research

Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems
This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203 (Source: Natural Product Research)
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research

Ergosterol peroxide: an effect-directed detecting method from < em > Anoectochilus elwesii < /em > and evaluation of anticancer activity
Nat Prod Res. 2021 Jul 13:1-6. doi: 10.1080/14786419.2021.1949593. Online ahead of print.ABSTRACTErgosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecti...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Yanduan Lin Bingqin Wen Ziwei Zhang Jinyan Cai Source Type: research

A blue enzyme from marine bacterium for green technological applications
Nat Prod Res. 2021 Jul 13:1-2. doi: 10.1080/14786419.2021.1948044. Online ahead of print.ABSTRACTLaccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular laccases as compared to fungal laccases. Therefore, there are many advantages of bacterial laccases over fungal laccases.PMID:34254841 | DOI:10.1080/14786419.2021.1948044 (Source: Natu...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Abraham P Karlapudi Vidya P Kodali Source Type: research

Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems
This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203 (Source: Natural Product Research)
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research

Ergosterol peroxide: an effect-directed detecting method from < em > Anoectochilus elwesii < /em > and evaluation of anticancer activity
Nat Prod Res. 2021 Jul 13:1-6. doi: 10.1080/14786419.2021.1949593. Online ahead of print.ABSTRACTErgosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecti...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Yanduan Lin Bingqin Wen Ziwei Zhang Jinyan Cai Source Type: research

A blue enzyme from marine bacterium for green technological applications
Nat Prod Res. 2021 Jul 13:1-2. doi: 10.1080/14786419.2021.1948044. Online ahead of print.ABSTRACTLaccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular laccases as compared to fungal laccases. Therefore, there are many advantages of bacterial laccases over fungal laccases.PMID:34254841 | DOI:10.1080/14786419.2021.1948044 (Source: Natu...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Abraham P Karlapudi Vidya P Kodali Source Type: research

Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems
This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203 (Source: Natural Product Research)
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research

Ergosterol peroxide: an effect-directed detecting method from < em > Anoectochilus elwesii < /em > and evaluation of anticancer activity
Nat Prod Res. 2021 Jul 13:1-6. doi: 10.1080/14786419.2021.1949593. Online ahead of print.ABSTRACTErgosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecti...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Yanduan Lin Bingqin Wen Ziwei Zhang Jinyan Cai Source Type: research

A blue enzyme from marine bacterium for green technological applications
Nat Prod Res. 2021 Jul 13:1-2. doi: 10.1080/14786419.2021.1948044. Online ahead of print.ABSTRACTLaccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular laccases as compared to fungal laccases. Therefore, there are many advantages of bacterial laccases over fungal laccases.PMID:34254841 | DOI:10.1080/14786419.2021.1948044 (Source: Natu...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Abraham P Karlapudi Vidya P Kodali Source Type: research

Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems
This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203 (Source: Natural Product Research)
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research

Ergosterol peroxide: an effect-directed detecting method from < em > Anoectochilus elwesii < /em > and evaluation of anticancer activity
Nat Prod Res. 2021 Jul 13:1-6. doi: 10.1080/14786419.2021.1949593. Online ahead of print.ABSTRACTErgosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecti...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Yanduan Lin Bingqin Wen Ziwei Zhang Jinyan Cai Source Type: research

A blue enzyme from marine bacterium for green technological applications
Nat Prod Res. 2021 Jul 13:1-2. doi: 10.1080/14786419.2021.1948044. Online ahead of print.ABSTRACTLaccases are the green tools that can find potential applications in various industries. There are many reports available on laccases from plants and fungal sources but very few reports are available on bacterial laccases. Bacterial laccases show broad range of substrate specificity and it is easy to isolate and purify the bacterial extracellular laccases as compared to fungal laccases. Therefore, there are many advantages of bacterial laccases over fungal laccases.PMID:34254841 | DOI:10.1080/14786419.2021.1948044 (Source: Natu...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Abraham P Karlapudi Vidya P Kodali Source Type: research

Extraction and purification of anthocyanins from < em > Peristrophe bivalvis < /em > (L.) < em > Merr. < /em > leaf (Acanthaceae) using aqueous two-phase systems
This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.PMID:34254860 | DOI:10.1080/14786419.2021.1952203 (Source: Natural Product Research)
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Phuong H Le Duy N Dao Tin Q Huynh Tra T T Tran Viet Nguyen Source Type: research

Ergosterol peroxide: an effect-directed detecting method from < em > Anoectochilus elwesii < /em > and evaluation of anticancer activity
Nat Prod Res. 2021 Jul 13:1-6. doi: 10.1080/14786419.2021.1949593. Online ahead of print.ABSTRACTErgosterol peroxide (EP) in Anoectochilus elwesii possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecti...
Source: Natural Product Research - July 13, 2021 Category: Biochemistry Authors: Yanduan Lin Bingqin Wen Ziwei Zhang Jinyan Cai Source Type: research

A new diterpenoid alkaloid from < em > Delphinium gyalanum < /em > C. Marquand & amp; Airy Shaw
Nat Prod Res. 2021 Jul 9:1-6. doi: 10.1080/14786419.2021.1948043. Online ahead of print.ABSTRACTA new C19-diterpenoid alkaloid named gyalanutine A (1) and fourteen known compounds 2-15 were isolated from the plant of Delphinium gyalanum C. Marquand & Airy Shaw. Compound 1 displayed an unusual lycoctonine-type C19-diterpenoid alkaloid skeleton with the cleavage of N-C19 and C7-C17 bonds, and the construction of the N-C7 bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.PMID...
Source: Natural Product Research - July 9, 2021 Category: Biochemistry Authors: Xinyu Li Meizhen Ye Feng Gao Xianli Zhou Lin Chen Shuai Huang Source Type: research

A new diterpenoid alkaloid from < em > Delphinium gyalanum < /em > C. Marquand & amp; Airy Shaw
Nat Prod Res. 2021 Jul 9:1-6. doi: 10.1080/14786419.2021.1948043. Online ahead of print.ABSTRACTA new C19-diterpenoid alkaloid named gyalanutine A (1) and fourteen known compounds 2-15 were isolated from the plant of Delphinium gyalanum C. Marquand & Airy Shaw. Compound 1 displayed an unusual lycoctonine-type C19-diterpenoid alkaloid skeleton with the cleavage of N-C19 and C7-C17 bonds, and the construction of the N-C7 bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.PMID...
Source: Natural Product Research - July 9, 2021 Category: Biochemistry Authors: Xinyu Li Meizhen Ye Feng Gao Xianli Zhou Lin Chen Shuai Huang Source Type: research

A new diterpenoid alkaloid from < em > Delphinium gyalanum < /em > C. Marquand & amp; Airy Shaw
Nat Prod Res. 2021 Jul 9:1-6. doi: 10.1080/14786419.2021.1948043. Online ahead of print.ABSTRACTA new C19-diterpenoid alkaloid named gyalanutine A (1) and fourteen known compounds 2-15 were isolated from the plant of Delphinium gyalanum C. Marquand & Airy Shaw. Compound 1 displayed an unusual lycoctonine-type C19-diterpenoid alkaloid skeleton with the cleavage of N-C19 and C7-C17 bonds, and the construction of the N-C7 bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.PMID...
Source: Natural Product Research - July 9, 2021 Category: Biochemistry Authors: Xinyu Li Meizhen Ye Feng Gao Xianli Zhou Lin Chen Shuai Huang Source Type: research

A new diterpenoid alkaloid from < em > Delphinium gyalanum < /em > C. Marquand & amp; Airy Shaw
Nat Prod Res. 2021 Jul 9:1-6. doi: 10.1080/14786419.2021.1948043. Online ahead of print.ABSTRACTA new C19-diterpenoid alkaloid named gyalanutine A (1) and fourteen known compounds 2-15 were isolated from the plant of Delphinium gyalanum C. Marquand & Airy Shaw. Compound 1 displayed an unusual lycoctonine-type C19-diterpenoid alkaloid skeleton with the cleavage of N-C19 and C7-C17 bonds, and the construction of the N-C7 bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.PMID...
Source: Natural Product Research - July 9, 2021 Category: Biochemistry Authors: Xinyu Li Meizhen Ye Feng Gao Xianli Zhou Lin Chen Shuai Huang Source Type: research

Antimicrobial and antioxidant activities of secondary metabolites from endophytic fungus < em > Botryosphaeria fabicerciana < /em > (MGN23-3) associated to < em > Morus nigra < /em > L
This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analysed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed toward...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Angela Aparecida da Silva Julio Cesar Polonio Aline Maria Bulla Andressa Domingos Polli Juliana Cristina Castro Lilian Capelari Soares Verci Alves de Oliveira-Junior Veronica Elisa Pimenta Vicentini Arildo Jos é Braz de Oliveira Jos é Eduardo Gonçalves Source Type: research

Essential oil variability in < em > Eugenia dysenterica < /em > fruits
Nat Prod Res. 2021 Jul 6:1-4. doi: 10.1080/14786419.2021.1947273. Online ahead of print.ABSTRACTEssential oils (EOs) of Eugenia dysenterica fruits from seven populations were assessed using GC/MS and chemometric analysis. Variations in EOs between populations and three operational chemical units (OCUs), combined with foliar Mn2+ as an environmental variable, indicate that 86.8% of variation in oils was explained by these predictors. Variance partitioning shows that the largest pure contribution was attributed to foliar Mn2+ (13.1%), followed by OCU (11.4%). Populational origin contributed with lowest variance (6.6%).PMID:3...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Eud écio Bonfim Dos Santos Dias Yanuzi Mara Vargas Camilo Eli Regina Barboza de Souza Pedro Henrique Ferri Source Type: research

Chemical characterisation and < em > in vitr < /em > o anthelmintic activity of phenolic-rich extracts from the leaves and branches of < em > Maytenus ilicifolia < /em > , a native plant from South America
Nat Prod Res. 2021 Jul 6:1-5. doi: 10.1080/14786419.2021.1948844. Online ahead of print.ABSTRACTFree (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Alth...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Cristina Olivaro Micaela Escobal Guillermo de Souza Am érica Mederos Source Type: research

The efficient synthesis and biological evaluation of justicidin B
Nat Prod Res. 2021 Jul 6:1-7. doi: 10.1080/14786419.2021.1948843. Online ahead of print.ABSTRACTA facile new synthetic method for the preparation of a Type-A 1-arylnaphthalene lactone skeleton was developed and used to synthesise justicidin B and several derivatives. Key synthesis steps included Hauser-Kraus annulation of a phthalide intermediate and Suzuki-Miyaura cross coupling between a triflated naphthalene lactone intermediate and various potassium organotrifluoroborates. With two exceptions, the derivatives showed significant inhibitory effect on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse ...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Taejung Kim Young-Joo Kim Kyu-Hyuk Jeong Young-Tae Park Hyukjoon Kwon Pilju Choi Ha-Neul Ju Cheol Hee Yoon Ji-Yool Kim Jungyeob Ham Source Type: research

Prebiotic effects of citrus pectic oligosaccharides
This study demonstrated that POS2 (product from the enzymatic hydrolysis by PEC2) showed greater prebiotic activity than POS1 (product from the enzymatic hydrolysis by PEC1). The results confirmed that citrus pectic oligosaccharides could be used as emerging prebiotics with improved properties due to their antimicrobial and modulating-microbiota ability.PMID:34227455 | DOI:10.1080/14786419.2021.1948845 (Source: Natural Product Research)
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Paola Foti Gabriele Ballistreri Nicolina Timpanaro Paolo Rapisarda Flora V Romeo Source Type: research

Antimicrobial and antioxidant activities of secondary metabolites from endophytic fungus < em > Botryosphaeria fabicerciana < /em > (MGN23-3) associated to < em > Morus nigra < /em > L
This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analysed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed toward...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Angela Aparecida da Silva Julio Cesar Polonio Aline Maria Bulla Andressa Domingos Polli Juliana Cristina Castro Lilian Capelari Soares Verci Alves de Oliveira-Junior Veronica Elisa Pimenta Vicentini Arildo Jos é Braz de Oliveira Jos é Eduardo Gonçalves Source Type: research

Essential oil variability in < em > Eugenia dysenterica < /em > fruits
Nat Prod Res. 2021 Jul 6:1-4. doi: 10.1080/14786419.2021.1947273. Online ahead of print.ABSTRACTEssential oils (EOs) of Eugenia dysenterica fruits from seven populations were assessed using GC/MS and chemometric analysis. Variations in EOs between populations and three operational chemical units (OCUs), combined with foliar Mn2+ as an environmental variable, indicate that 86.8% of variation in oils was explained by these predictors. Variance partitioning shows that the largest pure contribution was attributed to foliar Mn2+ (13.1%), followed by OCU (11.4%). Populational origin contributed with lowest variance (6.6%).PMID:3...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Eud écio Bonfim Dos Santos Dias Yanuzi Mara Vargas Camilo Eli Regina Barboza de Souza Pedro Henrique Ferri Source Type: research

Chemical characterisation and < em > in vitr < /em > o anthelmintic activity of phenolic-rich extracts from the leaves and branches of < em > Maytenus ilicifolia < /em > , a native plant from South America
Nat Prod Res. 2021 Jul 6:1-5. doi: 10.1080/14786419.2021.1948844. Online ahead of print.ABSTRACTFree (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Alth...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Cristina Olivaro Micaela Escobal Guillermo de Souza Am érica Mederos Source Type: research

The efficient synthesis and biological evaluation of justicidin B
Nat Prod Res. 2021 Jul 6:1-7. doi: 10.1080/14786419.2021.1948843. Online ahead of print.ABSTRACTA facile new synthetic method for the preparation of a Type-A 1-arylnaphthalene lactone skeleton was developed and used to synthesise justicidin B and several derivatives. Key synthesis steps included Hauser-Kraus annulation of a phthalide intermediate and Suzuki-Miyaura cross coupling between a triflated naphthalene lactone intermediate and various potassium organotrifluoroborates. With two exceptions, the derivatives showed significant inhibitory effect on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse ...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Taejung Kim Young-Joo Kim Kyu-Hyuk Jeong Young-Tae Park Hyukjoon Kwon Pilju Choi Ha-Neul Ju Cheol Hee Yoon Ji-Yool Kim Jungyeob Ham Source Type: research

Prebiotic effects of citrus pectic oligosaccharides
This study demonstrated that POS2 (product from the enzymatic hydrolysis by PEC2) showed greater prebiotic activity than POS1 (product from the enzymatic hydrolysis by PEC1). The results confirmed that citrus pectic oligosaccharides could be used as emerging prebiotics with improved properties due to their antimicrobial and modulating-microbiota ability.PMID:34227455 | DOI:10.1080/14786419.2021.1948845 (Source: Natural Product Research)
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Paola Foti Gabriele Ballistreri Nicolina Timpanaro Paolo Rapisarda Flora V Romeo Source Type: research

Antimicrobial and antioxidant activities of secondary metabolites from endophytic fungus < em > Botryosphaeria fabicerciana < /em > (MGN23-3) associated to < em > Morus nigra < /em > L
This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analysed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed toward...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Angela Aparecida da Silva Julio Cesar Polonio Aline Maria Bulla Andressa Domingos Polli Juliana Cristina Castro Lilian Capelari Soares Verci Alves de Oliveira-Junior Veronica Elisa Pimenta Vicentini Arildo Jos é Braz de Oliveira Jos é Eduardo Gonçalves Source Type: research

Essential oil variability in < em > Eugenia dysenterica < /em > fruits
Nat Prod Res. 2021 Jul 6:1-4. doi: 10.1080/14786419.2021.1947273. Online ahead of print.ABSTRACTEssential oils (EOs) of Eugenia dysenterica fruits from seven populations were assessed using GC/MS and chemometric analysis. Variations in EOs between populations and three operational chemical units (OCUs), combined with foliar Mn2+ as an environmental variable, indicate that 86.8% of variation in oils was explained by these predictors. Variance partitioning shows that the largest pure contribution was attributed to foliar Mn2+ (13.1%), followed by OCU (11.4%). Populational origin contributed with lowest variance (6.6%).PMID:3...
Source: Natural Product Research - July 6, 2021 Category: Biochemistry Authors: Eud écio Bonfim Dos Santos Dias Yanuzi Mara Vargas Camilo Eli Regina Barboza de Souza Pedro Henrique Ferri Source Type: research