A structure optimized fluorescent probe for highly sensitive monitoring drug induced lysosomal pH value changes.
Abstract Lysosomes generally maintain the weak acidic microenvironment, to ensure highly efficient activity and functions of hydrolytic enzymes and proteins. Aberrations of the lysosomal pH may result in cellular functional changes and influence human physiology, possibly causing serious diseases. Small-molecular fluorescent probes based imaging techniques capable of providing information on target locations are considerably appreciated. Herein, by reducing the size of the typical lysosome targetable group 4-(2-aminoethyl) morpholine, we rationally designed a rhodamine analogue probe Ly-HN2AM with N-Aminomorpholin...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Gong YJ, Kong ZZ, Zhang ML, Lv MK, Zhang G Tags: Talanta Source Type: research

Graphite sheet as a novel material for the collection and electrochemical sensing of explosive residues.
Abstract This work presents the use of a graphite sheet (graphite papers) as a new platform for the collection and sensing of explosive residues. This material offers a lightweight, highly conductive, flexible platform that can be cut in several ways, enabling for the collection of explosive residues at the place of interest, without any further sample preparation steps. As a proof-of-concept, the device was utilised for the collection and electrochemical sensing of 2,4,6-trinitrotoluene (TNT) residues. The GS has a remarkable performance for the sensing of TNT within the supporting electrolyte (0.1 mol dm-3 H...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Castro SVF, Cardoso RM, Santana MHP, Richter EM, Munoz RAA Tags: Talanta Source Type: research

Co3O4 nanoparticles supported mesoporous carbon framework interface for glucose biosensing.
Abstract The present work reports the preparation of advanced functional nanostructures based on cobalt oxide supported mesoporous carbon framework (Co3O4@MCF) for electrochemical biosensing. Co3O4@MCF was synthesized by simple hythrothermal & pyrolysis method and further characterized by various microscopic and spectroscopic techniques. The transmission electron microscopic (TEM) images show the lattice fringes of crystalline Co3O4 with interlayer spacing of 0.24 nm. The characteristic 311 plane in X-ray diffraction (XRD) studies further confirmed the presence of crystalline Co3O4 on carbon frameworks. Refl...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Dhanjai, Balla P, Sinha A, Wu L, Lu X, Tan D, Chen J Tags: Talanta Source Type: research

Attribution of fentanyl analogue synthesis routes by multivariate data analysis of orthogonal mass spectral data.
C, Larsson A, Östin A Abstract Chemical attribution signatures (CAS) can be used to obtain useful forensic information and evidence from illicit drug seizures. A CAS is typically generated using hyphenated chemical analysis techniques and consists of a fingerprint of the by-products and additives present in a sample. Among other things, it can provide information on the sample's origin, its method of production, and the sources of its precursors. This work investigates the possibility of using multivariate CAS analysis to identify the synthetic methods used to prepare seized fentanyl analogues, independently...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Mörén L, Qvarnström J, Engqvist M, Afshin-Sander R, Wu X, Dahlén J, Löfberg C, Larsson A, Östin A Tags: Talanta Source Type: research

Sensitive and cost effective disposable composite electrode based on graphite, nano-smectite and multiwall carbon nanotubes for the simultaneous trace level detection of ascorbic acid and acetylsalicylic acid in pharmaceuticals.
In this study, we used nano-smectite (SMT) and multiwall carbon nanotube (MWCNT) as a recognition surface over the disposable pencil graphite electrode for the low-level detection of ascorbic acid (AA) and acetyl salicylic acid (ACA). Firstly, nano-semectit was obtained by the purification of natural bentonite clay. XRD patterns and Scanning electron microscopy (SEM) were employed to ensure the purity of the bentonite samples. Pencil graphite rodes were immersed into suspensions of SMT, MWCNT or the both.The surface morphology of the sensor was investigated by cyclic voltammery (CV), electrochemical impedance spectroscopy ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Bayraktepe DE, Yazan Z, Önal M Tags: Talanta Source Type: research

Comprehensive comparison of ambient mass spectrometry with desorption electrospray ionization and direct analysis in real time for direct sample analysis.
Abstract Ambient ionization mass spectrometry is a powerful analytical method for direct analysis of raw samples. Desorption electrospray ionization (DESI) and direct analysis in real time (DART) are two pioneer ambient ionization techniques and have been widely used in various fields. Despite it is a fact that the application and development of DESI and DART are gaining significant attention in analytical science, few comprehensive comparisons of DESI and DART have been made for in-depth understanding their characteristics for direct sample analysis. In this work, substituent effect on DESI and DART were systemat...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Li L, Wang Q, Li W, Yao YN, Wu L, Hu B Tags: Talanta Source Type: research

Application of the "inverted chirality columns approach" for the monitoring of asymmetric synthesis protocols.
Application of the "inverted chirality columns approach" for the monitoring of asymmetric synthesis protocols. Talanta. 2019 Oct 01;203:147-152 Authors: Ianni F, Tiecco M, Carotti A, Pucciarini L, Saluti G, Galarini R, Sardella R, Natalini B Abstract In the present study, the "Inverted Chirality Columns Approach (ICCA)" was applied to follow an asymmetric synthetic reaction, namely, the addition of butan-2-one to trans-β-nitrostyrene, catalysed by (S)-proline, leading to the formation of 3-methyl-4-phenyl-5-nitro-2-pentan-2-one. The ICCA method was applied to overcome the lack...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Ianni F, Tiecco M, Carotti A, Pucciarini L, Saluti G, Galarini R, Sardella R, Natalini B Tags: Talanta Source Type: research

Early diagnosis of Zika infection using a ZnO nanostructures-based rapid electrochemical biosensor.
Abstract In this work, we report a develop of electrochemical immunosensor based on ZnO nanostructures immobilized with ZIKV-NS1 antibody on Printed Circuit Board (PCB). ZnO nanostructures were grown on PCB by chemical bath deposition (CDB) and characterized by SEM. ZIKV-NS1 antibody was immobilized on the ZnO nanostructures surface via cystamine and glutaraldehyde. The samples were characterized by Immunofluorescence Confocal Microscopy and FTIR to identify the immobilization of the antibody to the sensor board. The analytical responses of the immunosensor were evaluated by Cyclic Voltammetry (CV). The biosensor ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Faria AM, Mazon T Tags: Talanta Source Type: research

Effective extraction of flavonoids from Lycium barbarum L. fruits by deep eutectic solvents-based ultrasound-assisted extraction.
In this study, we addressed, for the first time, to give a fruitful illustration to extract bioactive compounds from favourite Lycium barbarum L. fruits using DESs as green designer solvents, and displaying the tuneability of DESs to extract bioactive compounds from biomass efficiently. Among the studied DESs, the 1 : 2 M mixture of choline chloride and p-toluene sulfonic acid (DESs-6) with an ultra-sound assisted extraction (UAE) method was more efficient to get high extraction yields of flavonoids including myricetin (57.2 mg/g), morin (12.7 mg/g), and rutin (9.1 mg/g) in net DESs. The resulting extraction method...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Ali MC, Chen J, Zhang H, Li Z, Zhao L, Qiu H Tags: Talanta Source Type: research

Simple impedimetric sensor for rapid lipase activity quantification.
Abstract A simple and rapid impedimetric sensor applicable for industrial lipase activity quantification was developed and characterized. It is based on the lipase catalyzed degradation by hydrolysis of a thin nanocomposite substrate sensitive layer deposited on a PCB stick electrode usable as disposable or regenerable. The sensitive layer degradation rate was evaluated by the impedance changes registration along time resulting from its thickness diminution applying a small amplitude AC voltage with a constant frequency. The AC current phase shift variations along the time caused by the impedance changes were regi...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Zlatev R, Stoytcheva M, Valdez B, Montero G, Toscano L Tags: Talanta Source Type: research

Magnetic nanostructures for preconcentration, speciation and determination of chromium ions: A review.
Abstract Magnetic nanoparticles based solid-phase extraction is a new analytical technique based on the use of magnetic sorbents for the preconcentration and quantification of different inorganic and organic species. The present review concentrates on recent developments that have been built in magnetic nanostructures-based solid phase extraction, speciation and quantification of chromium ions. Besides, a description of the preparation, characterization as well as applications of various types of magnetic nanostructures, either with an inorganic or organic coating of the magnetic core, is presented. In addition, t...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Filik H, Avan AA Tags: Talanta Source Type: research

A pyrazole-containing hydrazone for fluorescent imaging of Al3+ in lysosomes and its resultant Al3+ complex as a sensor for F.
Abstract A fluorescence-enhanced and lysosome-targeted Al3+ probe (1) based on a pyrazole-containing hydrazone was developed in this work. The fluorescent probe 1 displays an OFF-ON fluorescence response to Al3+ in the lysosome pH environment. In addition, the resultant 1-Al3+ complex could act as an ON-OFF fluorescence sensor for F-. Fluorescence imaging shows that 1 is suitable for visualization of Al3+ in lysosomes of HeLa cells. PMID: 31202324 [PubMed - in process] (Source: Talanta)
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Wang Y, Song YF, Zhang L, Dai GG, Kang RF, Wu WN, Xu ZH, Fan YC, Bian LY Tags: Talanta Source Type: research

Improved sensitivity and reproducibility in electrochemical detection of trace mercury (II) by bromide ion & electrochemical oxidation.
Improved sensitivity and reproducibility in electrochemical detection of trace mercury (II) by bromide ion & electrochemical oxidation. Talanta. 2019 Oct 01;203:186-193 Authors: Zou Y, Zhang Y, Xie Z, Luo S, Zeng Y, Chen Q, Liu G, Tian Z Abstract Nanostructured gold electrodes have been widely used for electrochemical trace analysis of Hg2+ because Hg2+ has superior specificity for the formation of gold amalgam. However, it is still very challenging to achieve both high sensitivity and reproducibility, especially for real environmental water samples due to surface poisoning by organic residues. In...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Zou Y, Zhang Y, Xie Z, Luo S, Zeng Y, Chen Q, Liu G, Tian Z Tags: Talanta Source Type: research

Authentication of the geographical origin of extra-virgin olive oil of the Arbequina cultivar by chromatographic fingerprinting and chemometrics.
chez I, Callao MP Abstract This paper proposes to use chromatographic fingerprints coupled to multivariate techniques to authenticate the geographical origin of extra-virgin olive oils (EVOO) of the Arbequina botanical variety. This methodology uses the whole or part of the chromatogram as input data for the classification models but does not identify or quantify the chemicals constituents. Arbequina monovarietal EVOOs from three geographical origins were studied: two from adjacent European Protected Designation of Origin areas, Siurana and Les Garrigues, in Catalonia in the northeast of Spain; and the third from ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Vera DN, Jiménez-Carvelo AM, Cuadros-Rodríguez L, Ruisánchez I, Callao MP Tags: Talanta Source Type: research

Label-free liquid crystal immunosensor for detection of HBD-2.
This study offers a simple, highly sensitive and specific, label -free method for HBD-2 detection. PMID: 31202327 [PubMed - in process] (Source: Talanta)
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Su X, Huo W, Yang D, Luan C, Xu J Tags: Talanta Source Type: research

Functionalized magnetic composites based on the aptamer serve as novel bio-adsorbent for the separation and preconcentration of trace lead.
Abstract A magnetic functionalized bio-sorbent based on aptamer was designed for the selective separation of ultra-trace Pb2+, shortly termed as Fe3O4@Au@DNA. Pb(II) specific aptamer attached to the magnetic solid substrate served as affinity probe to capture and separate trace lead. Oligonucleotides with a polyA block were employed for the immobilization on the surface of AuNPs, with adenine sequences (polyA) as the part of effective anchoring block. The prepared Fe3O4@Au@DNA composites were characterized by FT-IR, SEM and XPS. The binding of lead on Fe3O4@Au@DNA composites surface was pH-dependent, the adsorptio...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Li YK, Li WT, Liu X, Yang T, Chen ML, Wang JH Tags: Talanta Source Type: research

Label-free gold nanorods sensor array for colorimetric detection and discrimination of biothiols in human urine samples.
Abstract Biothiols play important roles in regulating redox balance in biological systems, but their discrimination is challengeable. In this work, a colorimetric nanosensing array for biothiols was established, which was composed of gold nanorods (AuNRs) and metal ions (Hg2+, Pb2+, Cu2+, Ag+). By employing label-free AuNRs as the colorimetric probe, and the color and spectral changes of AuNRs as the output signal, principal component analysis (PCA) was applied to processing the signal and generating a clustering map. Due to the different binding affinity between biothiols and metal ions, AuNRs exhibited a unique ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Yuan D, Liu JJ, Yan HH, Li CM, Huang CZ, Wang J Tags: Talanta Source Type: research

Facile synthesis of IrO2/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols.
Abstract In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxid...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Liu X, Wang X, Han Q, Qi C, Wang C, Yang R Tags: Talanta Source Type: research

Pattern recognition in voltammetric signals by ASD trilinear decomposition.
Abstract This work proposes a modern approach in separation of voltammetric current components which applies a pattern recognition strategy. Application of the trilinear decomposition algorithm ASD was first time demonstrated for detection and modelling of faradaic and capacitive components, recorded as total signal in DPV experiments. Using calibration set of the model substance i.e. K4Fe(CN)6, sampled with the frequency of 1 kHz, the signal components were extracted, which shape was consistent with the theoretic relations. Fitting in the area of peak expressed by r was 0.9957 for faradaic current and 0.9916 fo...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Ciepiela F, Jakubowska M Tags: Talanta Source Type: research

A screening method based on Visible-NIR spectroscopy for the identification and quantification of different adulterants in high-quality honey.
lma M, Barbero GF Abstract According to European Union regulations, honey is a pure product and adding to or removing from it any kind of substance is illegal. Nevertheless, its adulteration by adding inexpensive and artificial adulterants is a common practice. This paper deals with the use of visible and near-infrared spectroscopy (Vis-NIRS) combined with chemometric tools as a screening technique for the identification and quantification of different types of adulterants (inverted sugar, rice syrup, brown cane sugar and fructose syrup) added to high-quality honey (Granada Protected Designation of Origin, Spain) ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Aliaño-González MJ, Ferreiro-González M, Espada-Bellido E, Palma M, Barbero GF Tags: Talanta Source Type: research

Portable and low-cost biosensor towards on-site detection of diclofenac in wastewater.
ertig M Abstract Wastewater treatment plants are the main release sources of pharmaceutical compounds present in surface waters. Even at low concentrations, many of these substances have long-term adverse effects on the environment. For an efficient control of pharmaceutical removal, a real-time recognition is a prerequisite. Currently, quantification of such compounds is done in special equipped laboratories and is rather time-consuming and expensive. Here, we introduce a novel biosensor for the detection of the pharmaceutical compound diclofenac, which can be produced with low costs, is easy in handling and can ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Schirmer C, Posseckardt J, Schröder M, Gläser M, Howitz S, Scharff W, Mertig M Tags: Talanta Source Type: research

Metal-organic frameworks based fluorescent sensor array for discrimination of flavonoids.
Abstract Metal-organic frameworks (MOFs) are hotly investigated as a novel kind of fluorescent sensing materials in recent years. However, the application of MOF sensing to drug analysis is still very difficult yet, because the structural similarity among drug homolog-ues exceeds the discrimination ability of MOFs. Array sensing technique, which relies on the combined responses of a group of sensing materials, is a viable way to solve this problem. In this work, we chose five luminescent MOFs with different fluorophores to construct a fluorescent sensor array for the analysis of flavonoids. With the response patte...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Liu Q, Gao J, Zheng Z, Ning D, Wang Q, Du XM, Zhao B, Ruan WJ, Li Y Tags: Talanta Source Type: research

Exonuclease III-assisted strand displacement reaction-driven cyclic generation of G-quadruplex strategy for homogeneous fluorescent detection of melamine.
Abstract Development of flexible, sensitive, selective and simple melamine detection is in high demand and of great significance. Here, we proposed a homogeneous turn-off mode fluorescent strategy to detect melamine by coupling the process of exonuclease III-assisted (Exo III) amplification with the strand displacement reaction (SDR)-driven assembly of DNA G-quadruplex structures. Melamine (M) could bind to the thymine (T) base through hydrogen bonding to form a T-M-T structure, which inhibits the subsequent nucleic acid amplification reaction and formation of the G-quadruplex structure. DNA strand (G1) served as ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Chen P, Huang K, Zhang P, Sawyer E, Wu Z, Wei X, Ying B, Geng J Tags: Talanta Source Type: research

A nanocomposite fluorescent probe of polyaniline, graphene oxide and quantum dots incorporated into highly selective polymer for lomefloxacin detection.
Abstract A nanocomposite fluorescent probe based on fluorescence quenching was fabricated and utilized for the detection of lomefloxacin. The fabricated probe integrated the high sensitivity of quantum dots, the excellent selectivity of molecularly imprinted polymer and the high adsorption affinity of graphene oxide and polyaniline. The probe exhibited good sensitivity, high specificity, and rapidity for lomefloxacin monitoring. Fluorescence emission was reduced linearly by lomefloxacin from 0.10 to 50.0 μg L-1 and the probe exhibited a low limit of detection of 0.07 μg L-1. The nanooptosensor succes...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Orachorn N, Bunkoed O Tags: Talanta Source Type: research

Rapid and reversible adsorption of BTX on mesoporous silica thin films for their real time spectrophotometric detection in air at ppm levels.
ant M Abstract Benzene, toluene and xylenes (BTX) are among the most toxic volatile organic compounds that can be emitted into the environment of different workplaces. Regulations to protect workers continue to strengthen permissible exposure limit (PEL), and short-term exposure limit (STEL) for any 15-min period are defined for BTX that should be controlled with rapid, selective and sensitive on-site sensors. For that purpose, a real-time analysis of BTX is described here, using the UV spectrometric determination of instantaneously concentrated pollutants in a thin film of mesoporous and transparent silica deposi...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Hamdi K, Martin P, Etienne M, Hébrant M Tags: Talanta Source Type: research

Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device.
Abstract The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor. Optimization of adsorption time of mIgG revealed that both degrees of adso...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Toma K, Oishi K, Yoshimura N, Arakawa T, Yatsuda H, Mitsubayashi K Tags: Talanta Source Type: research

Electrochemical paper-based microfluidic device for high throughput multiplexed analysis.
Abstract A disposable microfluidic electrochemical paper-based device for multiplexed analysis based on sixteen independent microfluidic channels with electrochemical detection is proposed. A major advantage of this work was the non-necessary use of a wax printer for devices manufacturing which has a high cost of operation. In addition, a commercial multiplexing module was used that has the multiplexing capability of 8-16 channels and, for the first time using this module, the strategy of multiplexing both the working and reference electrodes were used. These sixteen channels with the respective sensors can be ope...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Fava EL, Silva TA, Prado TMD, Moraes FC, Faria RC, Fatibello-Filho O Tags: Talanta Source Type: research

Experiences with the uniqueness of Talanta.
Abstract Talanta has been known as one of the top ranking journals in (general) analytical chemistry. Applying a search term "Talanta" in Scopus, using the tool for analysis in there, some characteristics on Talanta were investigated. Tracing back for every 10 year period, by considering the citations relevant to the key words of the published papers, it can be seen that Talanta serves to report the works in the trends of that period. At the beginning period of Talanta, major contributions were from Europe and the USA and less contributions from Asia. After 30 years of Talanta, contributions from Asia, e...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Grudpan K, Paengnakorn P, Kiwfo K Tags: Talanta Source Type: research

In vivo bioluminescence imaging of labile iron pools in a murine model of sepsis with a highly selective probe.
Abstract Iron plays an essential role in biological system. An approach for in vivo imaging of this metal ion is needed to investigate its complex contributions to physiological and pathological processes. Herein, we present a bioluminescent probe FP-1 as a powerful tool for targeting Fe2+ detection in vitro and in vivo. The turn-on sensing scheme is based on the caged strategy of luciferin-luciferase system. FP-1 not only can detect accumulations of exogenous Fe2+ in living animal, but also has the capability of monitoring labile endogenous Fe2+ levels in animal model of sepsis. Implementation of this technique p...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Feng P, Ma L, Xu F, Gou X, Du L, Ke B, Li M Tags: Talanta Source Type: research

Combination of LEDs and cognitive modeling to quantify sheep cheese whey in watercourses.
Abstract The concentration of sheep cheese whey (CW) in water obtained from two Spanish reservoirs, two Spanish rivers, and distilled water has been estimated by combining spectroscopic measurements, obtained with light-emitting diodes (LEDs), and linear or non-linear algorithms. The concentration range of CW that has been studied covers from 0 to 25% in weight. Every sample was measured by six different types of LEDs possessing different emission wavelengths (blue, orange, green, pink, white, and UV). 1,800 fluorescence measurements were carried out and used to design different types of models to estimate the con...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Lastra-Mejias M, Villa-Martinez A, Izquierdo M, Aroca-Santos R, Cancilla JC, Torrecilla JS Tags: Talanta Source Type: research

Measurement of nutrients in saline and hypersaline waters by discrete analyzer colorimetry without matrix matched calibration standards.
Abstract Automated, colorimetric analysis of nutrients in samples with high and variable salinity can be time consuming due to the need to matrix match calibration and reference solution matrices with those of samples-particularly when using flow-based analyzers that are prone to detector artifacts caused by optical inhomogeneities, "schlieren," that form at interfaces between samples and deionized water carrier or wash solutions. Such detector artifacts do not occur in discrete analyzers. Here we report spike recoveries when nitrite plus nitrate, nitrite, ammonia, orthophosphate, and silica were determi...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Stetson SJ, Patton CJ, Guaglione N, Chestnut Z Tags: Talanta Source Type: research

Ionic liquid-based miniaturized aqueous biphasic system to develop an environmental-friendly analytical preconcentration method.
Afonso AM, Pino V Abstract Two ILs containing guanidinium cations (butylguanidinium chloride -C4Gu-Cl- and hexylguanidinium chloride -C6Gu-Cl-) were synthesized and characterized. Their cytotoxicity was also assessed, obtaining adequate CC50 values of 680 ± 99 mg·L-1 for C4Gu-Cl and 135 ± 8 mg·L-1 for C6Gu-Cl. Miniaturized aqueous biphasic systems (μ-ABSs) were developed using amounts lower than 1% (w/w) of these synthesized guanidinium-based ILs, K3PO4 as salting-out agent, and ultrapure water. The phases diagrams of both systems were determined, and the C4Gu-Cl-based &m...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: González-Martín R, Pacheco-Fernández I, Ayala JH, Afonso AM, Pino V Tags: Talanta Source Type: research

Sensitive monitoring of RNA transcription by optical amplification of cationic conjugated polymers.
We reported a new strategy for sensitive monitoring in vitro RNA synthesis in real time based on fluorescence resonance energy transfer (FRET) from water-soluble conjugated polymer poly (9, 9-bis (6'-N, N, N,-trimethylammonium) hexyl) fluorene-co-alt-1,4-phenylene) bromide (PFP) to fluorogenic RNA aptamer/fluorophore (Spanich2/DFHBI and Broccoli/DFHBI) system. In this strategy, RNA of interest was transcribed accompanied by the Spanich2 or Broccoli. Then the 3, 5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) bound to the RNA aptamer sequence and thereby induced a fluorescence signal. PFP was used as the fluorescence ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Li Z, Guo H, Xu F, Tang W, Duan X Tags: Talanta Source Type: research

Antimicrobial peptide arrays for wide spectrum sensing of pathogenic bacteria.
In this study, we describe the use of a biosensor for single-step bacteria detection. The whole analysis is performed in less than 20 h, during the growth phase of the micro-organisms, using an array of antimicrobial peptides (AMPs) coupled with a surface plasmon resonance imager (SPRI). A wide range of bacterial strains are assayed, showing differentiated affinity patterns with the immobilized peptides, which are confirmed by multivariate analysis. This work establishes the evidence that antimicrobial peptides, mostly used so far in the antibiotic drug industry, are suited for the wide-spectrum detection of unknown bact...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Pardoux É, Roux A, Mathey R, Boturyn D, Roupioz Y Tags: Talanta Source Type: research

Trends in the analysis of falsified and illegal medicines.
PMID: 31202347 [PubMed - in process] (Source: Talanta)
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Deconinck E Tags: Talanta Source Type: research

Development of the diffusive gradients in thin films technique (DGT) for platinum (Pt), palladium (Pd), and rhodium (Rh) in natural waters.
Abstract The Diffusive Gradients in Thin Films (DGT) technique was developed for the first time for the platinum group elements (PGEs) platinum (Pt), palladium (Pd), and rhodium (Rh). Different chelating resins, specific designed for the accumulation of PGEs, namely Purolite S914, S920, S985, Italmatch Chemicals IONQUEST® MPX-317 and MP-102, were compared. The method development involved several different steps: 1) selection of an appropriate diffusive gel, 2) comparison of resins for the PGEs in terms of kinetics of uptake, 3) development of an efficient elution method for the PGEs from the resin gel, 4) Dete...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Abdulbur-Alfakhoury E, Van Zutphen S, Leermakers M Tags: Talanta Source Type: research

Electrochemical enzyme-linked oligonucleotide array for aflatoxin B1 detection.
Abstract In this work, an electrochemical enzyme-linked oligonucleotide array to achieve simple and rapid multidetection of aflatoxin B1 (AFB1) is presented. The assay is based on a competitive format and disposable screen-printed cells (SPCs). Firstly, the electrodeposition of poly(aniline-anthranilic acid) copolymer (PANI-PAA) on graphite screen-printed working electrodes was performed by means of cyclic voltammetry (CV). Aflatoxin B1 conjugated with bovine serum albumin (AFB1-BSA) was then immobilized by covalent binding on PANI-PAA copolymer. After performing the affinity reaction between AFB1 and the biotinyl...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Selvolini G, Lettieri M, Tassoni L, Gastaldello S, Grillo M, Maran C, Marrazza G Tags: Talanta Source Type: research

Preparation of cotton wool modified with boric acid functionalized titania for selective enrichment of glycopeptides.
Abstract An effective enrichment technique is a prerequisite to enable comprehensive analysis of protein glycosylation. In this work, a method was developed for modifying cotton wool with boric acid-functionized titania nanomaterial. The modified cotton wool was used for the preparation of solid phase extraction pipette tips for glycopeptide enrichment. Owing to the unique properties of this novel solid phase material, an enrichment protocol was proposed by using a mixed-mode of hydrophilic interaction liquid chromatography, titania affinity chromatography and boronate affinity chromatography. The performance of t...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Liu L, Jin S, Mei P, Zhou P Tags: Talanta Source Type: research

Improved determination of femtogram-level organic explosives in multiple matrices using dual-sorbent solid phase extraction and liquid chromatography-high resolution accurate mass spectrometry.
Abstract Identification and trace quantification of multiple explosives residues, their precursors and transformation products in complex samples remains very challenging. For solid phase extraction (SPE) and liquid chromatography-high resolution accurate mass spectrometry-based methods (LC-HRMS), interferences from co-extracted matrix components can significantly affect recovery during extraction and/or detector signal. The aim of this work was to develop a new, improved and more generalisable extraction approach to trace explosives analysis in a range of matrices using dual-sorbent SPE with LC-HRMS. Recoveries o...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Irlam RC, Parkin MC, Brabazon DP, Beardah MS, O'Donnell M, Barron LP Tags: Talanta Source Type: research

Assembly of a UV-LED induced fluorescence system for rapid determination of amiloride in pharmaceutical tablet and human serum.
Abstract A simple and sensitive fluorescence method has been developed for the determination of amiloride (AMI) in pharmaceutical tablet and human serum with a portable, cost-effective, and easy-to-operate fluorescence system. The fluorescence system was assembled with some optical and electronic devices mainly including a 370 nm light-emitting-diode (LED) as light source, a fiber spectrometer and a Raspberry Pi computer. With the system, AMI produced a strong fluorescence emission at 413 nm, and the emitted intensity can maintain good stability with in a wide pH range from 2 to 8. The proposed method was succ...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Chen W, Xiong Y, Wang W, Wu T, Li L, Kang Q, Du Y Tags: Talanta Source Type: research

Smartphone assisted immunodetection of HIV p24 antigen using reusable, centrifugal microchannel array chip.
Abstract An integrated immunodetection platform employing a simple, reusable, centrifugal microchannel array chip and a smartphone as detection unit was developed. The applicability of the platform to the detection of HIV p24 antigen was demonstrated. The microchip was made of polycarbonate and contained 4 × 8 zigzag microchannels. After the monoclonal antibody of HIV p24 was adsorbed onto the channel surfaces, HIV p24 was introduced into the microchannel to react with the antibody. A biotin linked polyclonal antibody was then brought in to react with HIV p24, and SP80 (containing streptavidin and horser...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Li F, Zheng Y, Wu J, Zhao L, Shui L, Pu Q, Liu S Tags: Talanta Source Type: research

A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. colil-asparaginase.
We present here a SAW biosensor based approach for the functional quantification of Escherichia colil-asparaginase (E. colil-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. coli...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Yao H, Fernández CS, Xu X, Wynendaele E, De Spiegeleer B Tags: Talanta Source Type: research

Naphthalene-benzoindole derived two novel fluorometric pH-Responsive probes for environmental systems and bioimaging.
Abstract In this work, we designed a kind of novel fluorescent probes based on naphthalene-benzoindole conjugate derived for sensitively monitoring the pH fluctuation in environment and biological systems. In the spectral method, these two probes both show significant red shift, color change and fluorescence quenching due to the effect of intramolecular charge transfer (ICT) mechanism. Moreover, we found that the probe bearing with carboxyl group displayed a more hydrophilicity than the one bearing with hydrogen atom instead, and the former exhibits a faster response and higher fluorescence intensity. Besides, the...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Cheng S, Pan X, Shi M, Zuo G, Su T, Zhang C, Li J, Dong W Tags: Talanta Source Type: research

Characterization of white wines from north-eastern Italy with surface-enhanced Raman spectroscopy.
Abstract In the present study, label-free SERS spectroscopy is applied as a useful analytical technique for white wine characterization. 180 samples of three white wines varieties from northeastern Italy, Sauvignon Blanc, Ribolla Gialla and Friulano, collected from three different Italian producers from 2016 vintage, have been analyzed using Ag citrate-reduced colloids and a portable Raman instrument with a 785 nm laser. A PCA of SERS spectra showed that discrimination between wines and wineries is possible. Main spectral differences are due to adenine, carboxylic acids and glutathione, with their ratio changing...
Source: Talanta - June 18, 2019 Category: Chemistry Authors: Zanuttin F, Gurian E, Ignat I, Fornasaro S, Calabretti A, Bigot G, Bonifacio A Tags: Talanta Source Type: research

Development of deep eutectic solvent-based aqueous biphasic system for the extraction of lysozyme.
Abstract Aqueous Biphasic Systems (ABSs) based on deep eutectic solvents (DESs) were determined and applied in the extraction of lysozyme from chicken egg white. Tetrabutylammonium bromide (TBAB) and benzyltributylammonium bromide were utilized as hydrogen-bond acceptors to synthesize six kinds of DESs with different carboxylic acids (such as glycolic acid, Gly). The phase-formation ability of these DESs was evaluated by combining several salts. The results revealed that the content of hydrophilic group and the alkyl side chain length of the carboxylic acids played a crucial role in phase separation process, and t...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Xu P, Wang Y, Chen J, Wei X, Xu W, Ni R, Meng J, Zhou Y Tags: Talanta Source Type: research

Automated continuous-flow in-syringe dispersive liquid-liquid microextraction of mono-nitrophenols from large sample volumes using a novel approach to multivariate spectral analysis.
Švec F, Solich P Abstract Continuous magnetic stirring-assisted dispersive liquid-liquid extraction followed by dispersive backextraction as a novel pre-treatment technique for adaptable and milliliter volumes of environmental samples has been developed. The procedure was automated using the technique "Lab-In-Syringe". The void of the automated syringe pump was used as size-adaptable extraction chamber. By a flow channel in the syringe piston, continuous flow through the syringe void was enabled. 1-Octanol was used as an extractant and dispersed by the action of a magnetic stirring bar, which was ...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Fikarová K, Horstkotte B, Sklenářová H, Švec F, Solich P Tags: Talanta Source Type: research

Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer.
Abstract A new dual-modality immunosensor based on molecularly imprinted polymer (MIP) and a nanostructured biosensing layer has fabricated for the simultaneous detection of two important markers including prostate-specific antigen (PSA) and myoglobin (Myo) in human serum and urine samples. In the first step, 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) was self-assembled on a gold screen printed electrode (SPE). Then, the target proteins were attached covalently to the DSP-SPE. The imprinted cocktail polymer ((MIP(PSA, Myo)-SPE)) was synthesized at the SPE surface using acrylamide as monomer, ...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Karami P, Bagheri H, Johari-Ahar M, Khoshsafar H, Arduini F, Afkhami A Tags: Talanta Source Type: research

Recent progress in the development of recognition bioelements for polychlorinated biphenyls detection: Antibodies and aptamers.
Abstract Polychlorinated biphenyls (PCBs) are persistent pollutants, which have expanded in foods and the environment. Detection of PCBs is considered essential due to recognized side-effects of PCBs on health and the public concerns in this regard. On the other hand, due to the trace levels of these organic chlorine compounds, reliable and sensitive assays must be developed. Recognition elements are essential parts of analytical detection assays and sensors of PCBs since these elements are involved in the selective identification of the analytes of interest. Understanding the fundamentals of the recognition eleme...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Verdian A, Fooladi E, Rouhbakhsh Z Tags: Talanta Source Type: research

Novel sample-substrates for the determination of new psychoactive substances in oral fluid by desorption electrospray ionization-high resolution mass spectrometry.
Abstract A reliable screening and non invasive method based on the use of microextraction by packed sorbent coupled with desorption electrospray ionization-high resolution mass spectrometry was developed and validated for the detection of new psychoactive substances in oral fluid. The role of different sample substrates in enhancing signal intensity and stability was evaluated by testing the performances of two polylactide-based materials, i.e. non-functionalized and functionalized with carbon nanoparticles, and a silica-based material compared to commercially available polytetrafluorethylene supports. The best re...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Bianchi F, Agazzi S, Riboni N, Erdal N, Hakkarainen M, Ilag LL, Anzillotti L, Andreoli R, Marezza F, Moroni F, Cecchi R, Careri M Tags: Talanta Source Type: research

In situ self-transformation metal into metal-organic framework membrane for solid-phase microextraction of polycyclic aromatic hydrocarbons.
Abstract In this work, a metal-organic framework (MOF) membrane coated copper wire (CW) was prepared as solid-phase microextraction (SPME) materials for extraction of polycyclic aromatic hydrocarbons (PAHs) from water samples. The MOF based SPME fiber was fabricated by a facile and environment-friendly strategy, where the CW acted as both substrate and the copper ion source for MOF membrane growth. Characterization results confirmed that a uniform and dense HKUST-1 membrane was grown on the smooth surface of pristine CW. The effect of extraction parameters (i.e. extraction temperature, extraction time, desorption ...
Source: Talanta - June 8, 2019 Category: Chemistry Authors: Sun S, Huang L, Xiao H, Shuai Q, Hu S Tags: Talanta Source Type: research