Electronic classification of barcoded particles for multiplexed detection using supervised machine learning analysis.
Abstract Wearable biosensors are of great interest in recent years due to their potential in health related applications. Multiplex biomarker analysis is needed in wearable devices to improve the sensitivity and reliability. Electronic barcoding of micro-particles has the possibility to enable multiplexed biomarker analysis. Compared with traditional optical and plasmonic methods for barcoding, electronically barcoded particles can be classified using ultra-compact electronic readout platforms. Nano-electronic barcoding works by depositing a thin layer of oxide on the top half of a micro-particle. The thickness an...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Sui J, Xie P, Lin Z, Javanmard M Tags: Talanta Source Type: research

Nanozyme-based sensing platforms for detection of toxic mercury ions: An alternative approach to conventional methods.
Abstract Mercury (Hg) is known as a poisonous heavy metal which stimulates a wide range of adverse effects on the human health. Therefore, development of some feasible, practical and highly sensitive platforms would be desirable in determination of Hg2+ level as low as nmol L-1 or pmol L-1. Different approaches such as ICP-MS, AAS/AES, and nanomaterial-based nanobiosensors have been manipulated for determination of Hg2+ level. However, these approaches suffer from expensive instruments and complicated sample preparation. Recently, nanozymes have been assembled to address some disadvantages of conventional methods ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Hasan A, Nanakali NMQ, Salihi A, Rasti B, Sharifi M, Attar F, Derakhshankhah H, Mustafa IA, Abdulqadir SZ, Falahati M Tags: Talanta Source Type: research

Deep eutectic solvents (DES) as green extraction media for antioxidants electrochemical quantification in extra-virgin olive oils.
Abstract A new electroanalytical method has been developed for the determination of polar antioxidant compounds in extra virgin olive oils. This method is based on the extraction of polar antioxidant compounds from extra-virgin olive oils by means of a deep eutectic solvent and their determination by a modified screen-printed electrode platform. The platform sensitivity was increased by modifying the working electrode with MWCNT and TiO2 nanoparticles as modifiers and Nafion as a binder. The platform showed very good sensitivity in detecting polar antioxidant compounds in extra-virgin olive oils in a fairly wide r...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Shabani E, Zappi D, Berisha L, Dini D, Antonelli ML, Sadun C Tags: Talanta Source Type: research

An easily transferable protocol for in-situ quasi-non-invasive analysis of protein binders in works of art.
Abstract Proteomic approaches based on mass spectrometry have become increasingly popular for protein binder's identification in works of art. The identification of the binder employed may offer key information on paintings and other polychrome objects and contribute to assess their historical and technical context, also providing useful hints for a proper restoration and/or conservation treatment. Usually, the protocols employed to this purpose are invasive and at least micro sampling is required. Here, we present a simple transferable method for a quasi-non-invasive analysis of binders in artworks based on the u...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Calvano CD, Rigante E, Picca RA, Cataldi TRI, Sabbatini L Tags: Talanta Source Type: research

Development and validation of an advanced electrochemical sensor for the fast and cheap determination of hydrochlorothiazide in urine samples using the Monte-Carlo method for uncertainty evaluation.
Abstract This work describes the development, optimization, and validation of an electrochemical method for the determination of hydrochlorothiazide (HCTZ) in urine. The method allows fast, cheap and reliable determinations of recent administrations of this diuretic that can be used in doping control in sport. The response of the sensor was determined by differential pulse voltammetry (DPV). The glassy carbon electrode was modified with multiwall carbon nanotubes (MWCNT) and gold nanoparticles. The sensor is calibrated in the analysed sample matrix by the cumulative standard addition method. The method validation ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Galhardo KS, Dadamos TRL, Bettencourt da Silva RJN, Machado SAS Tags: Talanta Source Type: research

Characterization of interaction between Bcl-2 oncogene promoter I-Motif DNA and flavonoids using electrospray ionization mass spectrometry and pressure-assisted capillary electrophoresis frontal analysis.
Abstract B-cell lymphoma 2 (Bcl-2) is an antiapoptotic protein which is believed to be a triggering factor in developing human tumors. The Bcl-2 C-rich promoter element has been shown to form the i-motif (IM) via cytosine-cytosine (C-C+) base pair building blocks, which can be targeted through the binding of ligands associated with Bcl-2 expression modulation. In this work, we monitored IM development and thermodynamic stability within the Bcl-2 promoter via circular dichroism (CD) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The results demonstrated that at an acidic pH, as well as in a cr...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Yang Y, Fu H, Qian C, Li H, Chen DDY Tags: Talanta Source Type: research

A microfabricated thickness shear mode electroacoustic resonator for the label-free detection of cardiac troponin in serum.
Abstract Portable, and automated biosensors for the detection of cardiac biomarkers are important for the early diagnosis of acute myocardial infarction. We demonstrate a label-free microfabricated thickness shear mode electroacoustic device based on a ZnO piezoelectric film for the assay of cardiac biomarkers. This device combines an immunologic sensitive element with the resonator working in biological liquids. Based on the gravimetric mechanism, the immunoreaction between the target antigens and antibodies was detected by monitoring the frequency change in real-time to determine the concentration of cardiac tro...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Liu J, Chen D, Wang P, Song G, Zhang X, Li Z, Wang Y, Wang J, Yang J Tags: Talanta Source Type: research

Intramolecular trigger remodeling-induced HCR for amplified detection of protein-specific glycosylation.
Abstract Dynamic changes of protein-glycosylation on cell surface act as an important indicator that reflects cellular physiological states and disease developments. The enhanced visualization of protein-specific glycosylation is of great value to interpret its functions and mechanisms. Hence, we present an intramolecular trigger remodeling-induced hybridization chain reaction (HCR) for imaging protein-specific glycosylation. This strategy relies on designing two DNA probes, protein and glycan probes, labeled respectively on protein by aptamer recognition and glycan through metabolic oligosaccharide engineering (M...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Li Z, Yuan B, Lin X, Meng X, Wen X, Guo Q, Li L, Jiang H, Wang K Tags: Talanta Source Type: research

NiO@Ni-MOF nanoarrays modified Ti mesh as ultrasensitive electrochemical sensing platform for luteolin detection.
Abstract A novel electrochemical sensor was constructed based on three-dimensional NiO@Ni-MOF nanoarrays modified Ti mesh (NiO@Ni-MOF/TM). NiO nanoarrays were firstly produced on conductive TM using hydrothermal and carbonization method, and then Ni-MOFs were directly grown on the surface of NiO nanoarrays through self-template strategy. The morphology and structure of the prepared materials were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The as-prepared NiO@Ni-MOF/TM was used as elect...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Gao F, Tu X, Ma X, Xie Y, Zou J, Huang X, Qu F, Yu Y, Lu L Tags: Talanta Source Type: research

A novel highly sensitive and near-infrared fluorescent probe for detecting hypochlorite and its application in actual water sample and bioimaging.
Abstract Highly sensitive specific and ultrasensitive fluorescent probes are employed for tracking ClO⁻ for revealing its various cellular functions in living cells. In this work, a near-infrared fluorescence probe, XS-1, has been developed for tracking ClO⁻. The experimental results demonstrated that the probe, XS-1, could determine ClO⁻ at the linear range of 0-2 μM with a low detection limit of 72 nM (λex/em = 580/626 nm). XS-1 can specifically detect ClO⁻, which can be applied for determining the basal ClO⁻ and the fluctuation of exogenous ClO⁻ in living cells. F...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Xu L, Wu M, Zhao L, Han H, Zhang S, Ma P, Sun Y, Wang X, Song D Tags: Talanta Source Type: research

A hollow-structured nanohybrid: Intelligent and visible drug delivery and photothermal therapy for cancer.
Abstract It is of great valuable in drug delivery to fabricate multifunctional nanovehicle for cancer therapy. Herein, hollow-structured hCu2-xS@Au nanoshell/satellite composite with doxorubicin-carrying was designed and synthesized for intelligent and visible drug delivery and satisfactory photothermal therapy. By modification of disulfide linkage bridged Au nanoshell and multi-carboxylic graphene quantum dots (MC-GODs) on the surface of hCu2-xS@Au nanoparticles, both the high concentration of glutathione and low pH in cancer cell/tissue can induce responsive drug release. The satisfactory photothermal conversion...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Yang L, Hu B, Liu AH, Zhang Y Tags: Talanta Source Type: research

Application of 3D printed tools for customized open port probe-electrospray mass spectrometry.
Abstract Three dimensional printed open port probe (3DP-OPP) and air displacement based liquid handler, were designed and optimized using fused deposition modeling (FDM) and stereolitography (SLA) 3D printing. The performance of the devices were investigated for the analysis of solid and liquid samples with electrospray ionization mass spectrometry (ESI-MS). Direct analysis in less than 1 min and without any sample preparation, enabled detection of pesticides (azoxtystrobin/imazalil) on fruits peel surface and illegal substances (MDMA/MDEA) in home-made pills. Conjunction of OPP in the overspill mode with a c...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Sosnowski P, Hopfgartner G Tags: Talanta Source Type: research

Chemiluminescent microplate-based assay of DNA based on isothermal circular strand-displacement polymerization reaction (ICSDPR).
Abstract Sensitive microplate-based chemiluminescent assay coupled with isothermal circular strand-displacement polymerization reaction (ICSDPR) for DNA detection was developed. The assay sensitivity was improved using a triple amplification strategy based on employment of ICSDPR, streptavidin-polyperoxidase conjugate and an enhanced chemiluminescent reaction. To increase the accuracy all stages of the assay (one-pot format) were carried out in the same microplate well. The proposed assay detected the target DNA in fM-pM ranges and distinguish target DNA from related mismatched DNAs, demonstrating high sensitivity...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Solovjev AM, Kurzeev SA, Sakharov IY Tags: Talanta Source Type: research

Single-particle-frit-based packed columns for microchip chromatographic analysis of neurotransmitters.
Abstract The fabrication of effective microchip liquid chromatography (LC) systems tends to be limited by the availability of suitable chromatographic columns. Herein, we developed a glass microchip LC system in which porous single-particle silica was adopted as frits and a freeze-thaw valve was utilized to achieve sample injection without interfering with sampling. The fabrication of single-particle-frit-based packed columns did not require an additional packing channel, thus avoiding dead volumes within the channel interface that can influence chromatographic separation. Further, the length of the packed column ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Li K, Hu W, Zhou Y, Dou X, Wang X, Zhang B, Guo G Tags: Talanta Source Type: research

Label-free detection of cancer related gene based on target recycling and palindrome-mediated strand displacement amplification.
In this study, by combining the target recycling amplification (TRA) with strand displacement amplification (SDA), we have developed a label-free and highly sensitive method for the dual-amplified detection of STAT3. The assay system consists of polymerization primer and label-free hairpin probe (HP) containing palindromic fragment and nicking site. In the presence of STAT3, the stem of the HP is opened, followed by the primer binding to initiate TRA and SDA with the help of Klenow Fragment (KF) and nickase. After multiple replication, nicking, and strand displacement, STAT3 was released and initiated the next round of rea...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Xu H, Wu B, Wang J, Cao H, Yang J, Hao K, Chen S, Ye S, Shen Z Tags: Talanta Source Type: research

A telomerase-responsive nanoprobe with theranostic properties in tumor cells.
In this study, we report a novel telomerase-responsive nanoprobe with theranostic properties for inhibiting P-gp expression and reversing MDR by gene silencing. This nanoprobe is composed of an AuNP assembled with telomerase primer, antisense oligonucleotide (ASO), and doxorubicin (Dox). When the designed nanoprobe is uptaken by the MDR cancer cells, the Dox and ASO are specifically released due to the extension of telomerase primer triggered by telomerase. The released ASO specifically hybridizes with multidrug resistance 1 (MDR1) mRNA sequence, which encodes the P-gp. As a result, the expression of P-gp is inhibited and ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Zhang HH, Zhao LD, Zuo P, Yin BC, Ye BC Tags: Talanta Source Type: research

Label-free assay of protein kinase A activity and inhibition in cancer cell using electrochemically-prepared AuNP/rGO nanohybrid electrode modified with C-Kemptide.
Abstract A simple, label-free and sensitive electrochemical assay is described for the detection of protein kinase A (PKA) activity and inhibition in cancer cell by measuring the change in electrochemical impedance upon phosphorylation. The assay utilized gold nanoparticle (AuNP) and reduced graphene oxide (rGO) nanohybrid which was synthesized and deposited on the electrode from GO and Au3+ precursors by one-pot electrochemical synthesis. As-prepared AuNP/rGO electrode was employed to immobilize C-Kemptide peptide substrates which was phosphorylated in the presence of PKA and ATP. The resulting assay allowed effe...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Lee CS, Oh DE, Kim TH Tags: Talanta Source Type: research

A dual-stimulation strategy in a micro-chip for the investigation of mechanical associative learning behavior of C. elegans.
Abstract During the past decades, few micro-devices for analysis of associative learning behavior have been reported. In this work, an agarose-PDMS hybridized micro-chip was developed to establish a new associative learning model between mechanosensation and food reward in C. elegans. The micro-chip consisted of column arrays which mimicked mechanical stimulation to C. elegans. After trained by pairing bacterial food and mechanical stimuli in the chip, the worms exhibited associative learning behavior and gathered in the regions where there was food during training. The key research findings include: (1) Associati...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Wang Y, Wang X, Ge A, Hu L, Du W, Liu BF Tags: Talanta Source Type: research

A novel reaction-based fluorescence probe for rapid imaging of HClO in live cells, animals, and injured liver tissues.
Abstract Hypochlorous acid (HClO) is an important bioactive molecule, playing vital roles in a large number of physiological and pathological processes. Abnormal concentration of HClO in vivo has close contact with many diseases including inflammatory diseases and cancer. For bioimaging HClO, a new colorimetric and turn-on near-infrared (NIR) fluorescence probe (DDAO-ClO) was designed and synthesized in this work through a specific reaction of HClO with dimethylthiocarbamate. DDAO-ClO proved to show distinct and highly selective colorimetric and NIR fluorescence responses for HClO with fast response time (
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Deng Y, Feng S, Xia Q, Gong S, Feng G Tags: Talanta Source Type: research

Combining graphite with hollow-fiber liquid-phase microextraction for improving the extraction efficiency of relatively polar organic compounds.
In this study, we have developed a simple and effective hybrid extraction method based on the incorporation of raw carbon nanosorbents and octanol in the pores of a hollow-fiber membrane for improving the extraction efficiency of relatively polar organic compounds. Trihalomethanes (THMs) were used as model analytes. Three types of carbon nanosorbents (graphite, graphene, and multi-walled carbon nanotubes) were studied. The carbon sorbent incorporating membrane was used in a two-phase mode liquid-phase microextraction, with 1-octanol as the acceptor solution. Using a graphite-reinforced hollow-fiber membrane and an extracti...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Worawit C, Alahmad W, Miró M, Varanusupakul P Tags: Talanta Source Type: research

Alcohol-DES based vortex assisted homogenous liquid-liquid microextraction approach for the determination of total selenium in food samples by hydride generation AAS: Insights from theoretical and experimental studies.
Abstract This research article proposes a simple, quick, green and cheap approach for the determination of total selenium in food samples using alcohol-DES based vortex-assisted homogenous liquid-liquid microextraction (alcohol-DES-VA-HLLME) combination with hydride generation atomic absorption spectrometry (HG AAS). Analyte, complexing agent and working pH were Se(IV), Sudan-II and pH 4.0, respectively. In order to analyze the nature of the chemical interaction between Se(IV) ion and Sudan-II ligand, experimental results were supported with computational chemistry tools calculating quantum chemical descriptors li...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Altunay N, Elik A, Kaya S Tags: Talanta Source Type: research

Legionella pneumophila sg1-sensing signal enhancement using a novel electrochemical immunosensor in dynamic detection mode.
Abstract This work presents a comparison between static and dynamic modes of biosensing using a novel microfluidic assay for continuous and quantitative detection of Legionella pneumophila sg1 in artificial water samples. A self-assembled monolayer of 16-amino-1-hexadecanethiol (16-AHT) was covalently linked to a gold substrate, and the resulting modified surface was used to immobilize an anti-Legionella pneumophila monoclonal antibody (mAb). The modified surfaces formed during the biosensor functionalization steps were characterized using electrochemical measurements and microscopic imaging techniques. Under stat...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Laribi A, Allegra S, Souiri M, Mzoughi R, Othmane A, Girardot F Tags: Talanta Source Type: research

Cyanide chemosensors based on 3-dicyanovinylpyrazolo[1,5-a]pyrimidines: Effects of peripheral 4-anisyl group substitution on the photophysical properties.
Abstract Novel dual-mode colorimetric/fluorometric probes based on 3-dicyanovinylpyrazolo[1,5-a]pyrimidines for cyanide (CN-) sensing have been developed (DPPa-c). These probes displayed high selectivity and sensitivity toward CN- over other interfering anions, with a detection limit (LOD) as low as 610/170 nmol L-1 (absorption/emission) for some of the prepared probes. After a reaction with CN-, low-fluorescent DPPa-c showed a significant decrease of the intramolecular charge transfer (ICT) bands at approximately 390 nm (color changes from yellow to colorless) and exhibited up to an 82-fold fluores...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Tigreros A, Castillo JC, Portilla J Tags: Talanta Source Type: research

Humidity sensing performance of hybrid nanorods of polyaniline-Yttrium oxide composite prepared by mechanical mixing method.
Abstract In this work, polyaniline (PANI) synthesized by in-situ polymerization was mechanically mixed with different feeding mass ratios of Y2O3 to form four Polyaniline/Yttrium oxide (PYO) composites. Among them, the PYO-4 composite acquiring nanorod structure with high aspect ratio confirmed from its TEM image and consequent maximum enhancement in its humidity sensing response discussed in comparison with those of PANI and other composites. The interaction of PANI with Y2O3 in the composite confirmed from FTIR studies. The increase in defects and vacancies in all the composites, being maximum in PYO-4 composite...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Chethan B, Raj Prakash HG, Ravikiran YT, Vijaya Kumari SC, Manjunatha S, Thomas S Tags: Talanta Source Type: research

A simple and effective dansyl acid based "turn-on" fluorescent probe for detecting labile ferrous iron in physiological saline and live cells.
A simple and effective dansyl acid based "turn-on" fluorescent probe for detecting labile ferrous iron in physiological saline and live cells. Talanta. 2020 Aug 01;215:120908 Authors: Gao G, Wang X, Wang Z, Jin X, Ou L, Zhou J, Xie P Abstract Labile ferrous iron (Fe2+) plays important biochemical functions in many physiologically essential processes. It is very important to find an effective method to detect Fe2+. Herein, a simple and effective Fe2+ fluorescent probe (FeP1) has been constructed via a unique strategy of Fe2+-induced reducing reaction. As expected, FeP1 exhibited a 'turn-on' f...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Gao G, Wang X, Wang Z, Jin X, Ou L, Zhou J, Xie P Tags: Talanta Source Type: research

Highly sensitive impedimetric immunosensor for determination of interleukin 6 as a cancer biomarker by using conjugated polymer containing epoxy side groups modified disposable ITO electrode.
In conclusion, the proposed impedimetric immunosensor could be clinically useful in the early diagnosis of the prostate cancer by detection of the serum samples after only simple dilution with phosphate buffer. PMID: 32312453 [PubMed - in process] (Source: Talanta)
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Aydın EB Tags: Talanta Source Type: research

Solid-phase microextraction coatings based on the metal-organic framework ZIF-8: Ensuring stable and reusable fibers.
;guez-Hermida S, Pino V Abstract Chemical vapor deposition of MOFs (MOF-CVD) has been used to coat solid-phase microextraction (SPME) fibers with ZIF-8, by exposing ZnO layers to the linker vapor (2-methylimidazole). This ZIF-8 coating has been used as a seed layer in a following solvothermal MOF growth step in order to increase the ZIF-8 thickness. The combined MOF-CVD and solvothermal growth of ZIF-8 on the fibers result in a thickness of ~3 μm, with adequate thermal stability, and mechanical integrity when tested with methanol and acetonitrile ultrasonic treatments. The fibers have been evaluated in dir...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Rocío-Bautista P, Gutiérrez-Serpa A, Cruz AJ, Ameloot R, Ayala JH, Afonso AM, Pasán J, Rodríguez-Hermida S, Pino V Tags: Talanta Source Type: research

Identification of invisible biological traces in forensic evidences by hyperspectral NIR imaging combined with chemometrics.
Abstract The importance of detecting minute biological traces in forensic evidences feeds the continuous interest towards the development of new dedicated technologies both sensitive and reliable. The present study describes the opportunity to combine chemical properties derived from NIR signals with spatial features typical of RGB images by means of hyperspectral imaging (HSI). An analytical procedure based on HSI data collection and their multivariate processing followed by normalized difference images (NDI) is proposed as a screening method to highlight otherwise invisible traces of biological fluids on differe...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Malegori C, Alladio E, Oliveri P, Manis C, Vincenti M, Garofano P, Barni F, Berti A Tags: Talanta Source Type: research

Different strategies for class model optimization. A comparative study.
Abstract The Class Modelling (CM) approaches like Soft Independent Modelling of Class Analogy (SIMCA) aim at developing a mathematical model for determination of belongingness of new samples to the studied classes. The main feature of CM is that for each target class an individual model is constructed. CM is widely exploited, e.g., in the food and drug quality testing and authenticity or origin verification. It is well known that the most critical stage in construction of a class model is optimization of its parameters. There exist two basic strategies for optimization of class model, i.e., the "compliant&quo...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Małyjurek Z, Vitale R, Walczak B Tags: Talanta Source Type: research

A novel surface-enhanced Raman scattering-based ratiometric approach for detection of hyaluronidase in urine.
Abstract A ratiometric surface-enhanced Raman scattering (SERS) based method is described for the determination of the activity of hyaluronidase (HAase). Gold nanorods (AuNRs) were functionalized with 4-thiobenzonitrile (TBN) to act as the Raman reporter (TBN-AuNRs), and 4-thiophenylacetylene-functionalized gold-silver alloy nanoparticles (TPA-AuAgNPs) were used as the reference. Hyaluronic acid (HA) acts as the HAase recognition element. The TBN-modified AuNRs aggregate in the presence of HA due to the strong electrostatic interaction between the positively charged TBN-AuNRs and negatively charged HA. This strong...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Si Y, Li L, He B, Li J Tags: Talanta Source Type: research

NIR fluorescent probe based on a modified rhodol-dye with good water solubility and large Stokes shift for monitoring CO in living systems.
Abstract Because of the biological importance of CO, the development of effective probes for the detection of CO in living systems is of great significance. In particular, near-infrared (NIR) fluorescence probes with good water solubility and large Stokes shift are indispensable tools for CO detection in vivo. However, such tools are extremely scarce. Herein, a modified rhodol dye was used to develop a novel NIR fluorescent probe (Rh-NIR-CO) for selective and sensitive detection of CO using the Pd0-mediated Tsuji-Trost reaction. This probe shows good water solubility and rapid CO detecting ability in aqueous buffe...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Hong J, Xia Q, Zhou E, Feng G Tags: Talanta Source Type: research

Sandwich-like, potassium(I) doped g-C3N4 with tunable interlayer distance as a high selective extractant for the determination of Ba(II).
Abstract Element doping is considered as an effective strategy to adjust the interlayer distance of graphitic carbon nitride (g-C3N4) and the element doped g-C3N4 can be applied in metal extraction. Here, a series of potassium(I) doped graphitic carbon nitride (g-C3N4-K) materials with modulated interlayer distance ranging from 3.257 Å to 3.198 Å were successfully synthesized by calcining the different doped ratios mixture of melamine and KOH. Interestingly, g-C3N4-K (Q(melamine):Q(KOH) = 7:1) composite achieves the optimal properties and displays excellent extraction capacity and s...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Kang JY, Ha W, Zhang HX, Shi YP Tags: Talanta Source Type: research

Getting inside on virgin olive oil (VOO) photooxidation kinetics through combined generalized 2D correlation analysis and moving window 2D correlation analysis of ATR-FTIR spectra.
Abstract The generalized two-dimensional correlation spectroscopy (g2D-COR spectroscopy) analysis combined with its extended version moving-window 2D-COR (MW-2D-COR) was performed on a set of kinetic ATR-FTIR spectral data. The former is a mathematical method that is applied to a set of spectra collected for a sample exposed to an external perturbation (e.g. storage time). Virgin olive oils (VOOs) of similar fatty acid composition, chlorophyll, and a-tocopherol content were photooxidized (10,912 Lx, no headspace, 24 h). The proposed approach revealed hidden information under specific ATR-FTIR bands and the or...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Matsakidou A, Papadopoulou D, Nenadis N, Tsimidou MZ Tags: Talanta Source Type: research

An amplification strategy for detecting HER2 with a quasi-targeted proteomics approach coupled with aptamer-triggered hybridization chain reaction.
Abstract Human epidermal growth factor receptor 2 (HER2)-positive is a particularly aggressive type of the breast cancer. Because of the evidence has revealed that accurate HER2 status detection is crucial for prognosis and treatment strategy selection, great effort has been taken to develop assays for sensitive and accurate quantification of HER2. However, nonspecific amplification effect of most current assays limits the quantification accuracy of low abundance HER2. In the present work, we developed an LC-MS/MS-based quasi-targeted proteomics strategy coupled with hybridization chain reaction (HCR) for amplific...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Bao J, Zhang W, Zhou W, Lv M, Lu C, Yu H Tags: Talanta Source Type: research

Corrigendum to "Rational design of MnO nanoparticles assemblies for sensitive magnetic relaxation detection of melamine" [Talanta 208 (2020) 120359].
Corrigendum to "Rational design of MnO nanoparticles assemblies for sensitive magnetic relaxation detection of melamine" [Talanta 208 (2020) 120359]. Talanta. 2020 Aug 01;215:120919 Authors: Wang M, Zhao W, Lei S, Zou W, Hu W, Liu H, Guo Z, Ren L, Zhou X, Chen Z PMID: 32312462 [PubMed - in process] (Source: Talanta)
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Wang M, Zhao W, Lei S, Zou W, Hu W, Liu H, Guo Z, Ren L, Zhou X, Chen Z Tags: Talanta Source Type: research

Accurate quantification of TiO2 nanoparticles in commercial sunscreens using standard materials and orthogonal particle sizing methods for verification.
In this study, we evaluated three sample preparation methods to identify and quantify TiO2 nanoparticles in sunscreens. Specifically, we used a combination of ultracentrifugation and hexane washing, thermal destruction of the matrix, and surfactant assisted particle extraction. The method accuracy was assessed by two internal reference samples: pristine TiO2 nanoparticles (NM104) and similar TiO2 nanoparticles dispersed in a sunscreen matrix. The PSDs were determined using an asymmetrical flow field-flow fractionation hyphenated with multi-angle light scattering and inductively coupled plasma-mass spectroscopy. Particle si...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Velimirovic M, Wagner S, Monikh FA, Uusimäki T, Kaegi R, Hofmann T, Kammer FV Tags: Talanta Source Type: research

Wearable non-invasive monitors of diabetes and hypoxia through continuous analysis of sweat.
We present here wearable devices for continuous monitoring of diabetes and hypoxia based on continuous analysis of sweat. To induce sweating the clinically relevant procedure (pilocarpine electrophoresis) is used. Being a sufficient requirement for diagnostics, positive correlations in variation rates between glucose and lactate concentrations in sweat and the corresponding values in blood are shown. Continuous monitoring of human condition is possible only with the use of flow-through wearable devices providing a delivery of sweat to the biosensor almost immediately after secretion. Evaluating blood glucose through contin...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Karpova EV, Karyakina EE, Karyakin AA Tags: Talanta Source Type: research

Cobalt oxyhydroxide nanoflakes with oxidase-mimicking activity induced chemiluminescence of luminol for glutathione detection.
Abstract Cobalt oxyhydroxide nanoflakes (CoOOH NFs), a typical two-dimensional (2D) nanomaterials, were found to induce chemiluminescence (CL) of luminol since the oxidase-like activity of CoOOH NFs enables the dissolved oxygen to generate various radicals (OH, O2-and 1O2) even if without the addition of oxidants such as hydrogen peroxide, and these radicals could be scavenged by glutathione (GSH), making GSH could be detected sensitively in the range of 10 nM to 1 μM with the detection limit of 6.4 nM. PMID: 32312465 [PubMed] (Source: Talanta)
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Yang XJ, Li RS, Li CM, Li YF, Huang CZ Tags: Talanta Source Type: research

Selective and sensitive analysis by reactive easy ambient sonic-spray ionization: Synergistic combination of non-polar spray solvent and dicationic ionic liquid.
Abstract The present study reports the first conception to incorporate a non-polar solvent, dichloromethane, as the spray solution in easy ambient sonic-spray ionization (EASI) for mass spectrometry (MS) analysis of hydrophobic compounds. An imidazolium-based dicationic ionic liquid (DIL) at a low concentration of 20 μM was used in combination with dichloromethane. A reactive EASI strategy was implemented, by which the overall positively charged associated complexes of anionized perfluorinated compounds (PFCs) were formed in the presence of the DIL. In positive ionization mode, 19- and 6-fold enhancements ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Lv Y, Bai H, He Y, Yang J, Ma Q Tags: Talanta Source Type: research

Ampholine immobilized polymer microspheres for increasing coverage of human urinary proteome.
This study reports a convenient strategy for urine proteome analysis through ampholine immobilized polymer microsphere (ampholine@PM) fractionation strategy. After ampholine@PM treatment, 16,543 unique peptides corresponding to 2173 non-redundant urinary proteins were identified, while only 856 proteins, corresponding to 3524 peptides were identified in the crude urine sample. The number of proteins and peptides was increased by 1.54 and 3.69 times, respectively. 31 urinary candidate biomarkers have also been identified (17 candidate biomarkers of glomerular injury and 14 candidate biomarkers of tubular injury), showing th...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Deng N, Chen Y, Liang Z, Bian Y, Wang B, Sui Z, Zhang X, Yang K, Zhang L, Zhang Y Tags: Talanta Source Type: research

Magnetic N-doped 3D graphene-like framework carbon for extraction of cephalexin monohydrate and ceftiofur hydrochloride.
Abstract Magnetic N-doped 3D graphene-like framework carbon (Fe3O4@N-3DFC) was prepared via direct pyrolysis of sodium citrate and further hydrothermal reaction, and employed in the extraction of two cephalosporin antibiotics including cephalexin monohydrate and ceftiofur hydrochloride. The scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy and X-ray diffraction were employed to confirm the successful synthesis of 3DFC-based adsorbents (3DFCs) including 3DFC, Fe3O4@3DFC and Fe3O4@N-3DFC. Fe3O4@N-3DFC sorbent with 3D graphene-lik...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Niu P, Nie X, Li Y, Liang X, Wang L, Guo Y Tags: Talanta Source Type: research

Hydrophilic molecularly imprinted nanospheres for the extraction of rhodamine B followed by HPLC analysis: A green approach and hazardous waste elimination.
Abstract In this work, hydrophilic molecularly imprinted nanospheres (MINs) were synthesized via surface imprinting technology and subsequently utilized as dispersant sorbent in matrix solid phase dispersion (MSPD) for the extraction of rhodamine B (RhB) as the illogical food additive dye from different foodstuffs, followed by HPLC analysis. By considering hydrophilicity of target analyte, the sol-gel route was chosen for the preparation of MINs in aqueous media at mild conditions. Most importantly, both synthesis and extraction steps were designed and performed in the line of green chemistry and hazardous waste w...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Arabi M, Ostovan A, Bagheri AR, Guo X, Li J, Ma J, Chen L Tags: Talanta Source Type: research

A novel highly selective fluorescent probe with new chalcone fluorophore for monitoring and imaging endogenous peroxynitrite in living cells and drug-damaged liver tissue.
In this study, a novel turn-on fluorescent probe IC-ONOO with new chalcone fluorophore was designed and synthesized for the detection of in vitro and in vivo. The probe responded rapidly towards ONOO- (only within 15 min did the fluorescent intensity maximize), and was endowed with high sensitivity and excellent selectivity. Given the fact that the linear correlation between the fluorescent intensity at 560 nm and the concentrations of the probe ranged from 0 to 9 μM, the limit of detection (LOD) was calculated to be 3.1 × 10-8 M. With all the merits, probe IC-ONOO was qualified as a ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Ling C, Cui M, Chen J, Xia L, Deng D, Gu Y, Wang P Tags: Talanta Source Type: research

Colorimetric sensor array for accurate detection and identification of antioxidants based on metal ions as sensor receptors.
Abstract There is an ongoing need to develop high-performance sensing strategy for detecting and discriminating antioxidants, primarily because of their role in medical diagnosis and food. In this regard, visual sensor arrays have been a subject of intensive research for such applications. To this end, we propose a colorimetric sensor array for accurate detection and identification of antioxidants, which is based on the reactions between 3,3',5,5'-tetramethylbenzidine (TMB) and metal ions as sensing receptors and the interactions between antioxidants and oxidized TMB (oxTMB). Different target antioxidants displaye...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Zhang X, Liu Q, Chen Z, Zuo X Tags: Talanta Source Type: research

Indirect voltammetric determination of thiomersal in influenza vaccine using photo-degradation and graphene quantum dots modified glassy carbon electrode.
Aucelio RQ Abstract Thiomersal is an organomercury derivative that degrades producing thiosalicylic acid, dithiobenzoic acid and ethylmercury. It is widely used in topical pharmaceutical preparations and as preservative in vaccines and cosmetics. In this work, an electro-analytical method for thiomersal was developed using graphene quantum dots (GQDs) as a surface modifier of a glassy carbon electrode. The method rely on using square-wave voltammetry and exploring the synergistic effect between GQDs, visible radiation and the applied potential in producing very intense Hg oxidation peak during the anodic scan. A ...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Pedrozo-Penafiel MJ, Miranda-Andrades JR, Gutierrez-Beleño LM, Larrudé DG, Aucelio RQ Tags: Talanta Source Type: research

Effective validation of chromatographic analytical methods: The illustrative case of androgenic steroids.
Abstract The increasing need to develop quantitative chromatographic methods with upgradable multi-targeted approach, allowing flexible and reliable application on large daily workload makes the implementation of an efficient strategy of method's validation and maintenance crucial for the quality assurance policy. The expounding case of a gas chromatographic-mass spectrometric method for the urinary endogenous steroid profiling is presented to illustrate a validation strategy that combines rigorous estimation of validation parameters with highly efficient use of the collected data. The analysis of blank urine samp...
Source: Talanta - April 23, 2020 Category: Chemistry Authors: Alladio E, Amante E, Bozzolino C, Seganti F, Salomone A, Vincenti M, Desharnais B Tags: Talanta Source Type: research

Aptamer-based biosensor for detecting carcinoembryonic antigen.
Abstract Carcinoembryonic antigen (CEA), as one of the common tumor markers, is a human glycoprotein involved in cell adhesion and is expressed during human fetal development. Since the birth of human, CEA expression is largely inhibited, with only low levels in the plasma of healthy adults. Generally, CEA will overexpressed in many cancers, including gastric, breast, ovarian, lung, and pancreatic cancers, especially colorectal cancer. As one of the important tumor markers, the detection of CEA has great significance in differential diagnosis, condition monitoring and therapeutic evaluation of diseases. Convention...
Source: Talanta - April 13, 2020 Category: Chemistry Authors: Xiang W, Lv Q, Shi H, Xie B, Gao L Tags: Talanta Source Type: research

Biosensing strategies based on organic-scaffolded metal nanoclusters for ultrasensitive detection of tumor markers.
Abstract The recent rapid advances in the synthesis, functionalization and application of nanomaterials have enabled scientists to develop metal nanoclusters (MNCs) stabilized with a variety of scaffolds/protecting ligands including thiols, polymers, proteins, dendrimers and nucleic acids. Considering the unique optical, electronic and physical properties of MNCs, they have been successfully used for the tumor marker biosensing assays. In recent years, the ultrasensitive and accurate detection of tumor markers has been of critical importance for the screening or diagnosis of cancers at their early stages. Nanoclus...
Source: Talanta - April 13, 2020 Category: Chemistry Authors: Farzin L, Shamsipur M, Samandari L, Sadjadi S, Sheibani S Tags: Talanta Source Type: research

Covalent organic framework derived Fe3O4 / N co-doped hollow carbon nanospheres modified electrode for simultaneous determination of biomolecules in human serum.
Abstract In this work, Fe3O4/N co-doped hollow carbon spheres (Fe3O4@NHCS) as a promising electrocatalysis material had been prepared through carbonizing covalent organic frameworks and ferric irons. The morphology, structure, composition and electrocatalytic performance of Fe3O4@NHCS were characterized by various techniques. The electrode modified with Fe3O4@NHCS (Fe3O4@NHCS/GCE) exhibited excellent electrocatalytic activity for the oxidation of dopamine, uric acid, guanine and adenine. Simultaneous determination of these biomolecules was successfully achieved with Fe3O4@NHCS/GCE. Under the optimum conditions, th...
Source: Talanta - April 13, 2020 Category: Chemistry Authors: Lu Z, Shi Z, Huang S, Zhang R, Li G, Hu Y Tags: Talanta Source Type: research

Performance evaluation of a new three-in one diffusive sampler for monitoring NO2, SO2 and O3.
ml;, Döğeroğlu T Abstract A tailor-made diffusive sampler has been developed for the determination of nitrogen dioxide (NO2), sulfur dioxide (SO2) and ozone (O3) concentrations with a performance evaluation of the sampler being carried out under field and laboratory conditions. The most important characteristics of the sampler design is that simultaneous sampling of the three pollutants can be performed in one sampler. All the parts of the diffusive sampler are reusable after cleaning. These properties provide important advantages in terms of cost and practicality. Two alternative samplers, having long and ...
Source: Talanta - April 13, 2020 Category: Chemistry Authors: Üzmez ÖÖ, Gaga EO, Ercan Ö, Ceylan Ö, Döğeroğlu T Tags: Talanta Source Type: research