Label-free differentiation and quantification of ricin, abrin from their agglutinin biotoxins by surface plasmon resonance
Talanta. 2022 Feb 1;238(Pt 1):122860. doi: 10.1016/j.talanta.2021.122860. Epub 2021 Sep 9.ABSTRACTHere we describe an affinity molecule-directed surface plasmon resonance (SPR) immunosensor for a label-free, differentiation and quantification of ricin and abrin from their structural highly like agglutinin biotoxins. By an introduction of protein G as the affinity capturing molecule, we fulfilled a complete strategy contains (i) screening monoclonal antibodies to be paired in a sandwiched format, (ii) differentiate quantification from the agglutinin, (iii) ascertain of active from inactive biotoxin, and (iv) structural iden...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Li Luo Jiewei Yang Zhi Li Hua Xu Lei Guo Lili Wang Yuxia Wang Longlong Luo Jing Wang Pingping Zhang Ruifu Yang Weijun Kang Jianwei Xie Source Type: research

Structure and function encoding of a bidirectional activatable synergetic DNA machine for speeded and ultrasensitive determination of microRNAs
Talanta. 2022 Feb 1;238(Pt 1):123037. doi: 10.1016/j.talanta.2021.123037. Epub 2021 Nov 5.ABSTRACTThis work describes the unique design of a bidirectional activatable synergetic DNA machine (BAS-DNA machine) for speeded and ultrasensitive determination of microRNA-21 (miR-21), a well-known biomarker for biomedical research and early diagnosis of lung cancer. The BAS-DNA machine is composed by a pair of track strands (Track 1 and Track 2) encoding with two regions in the opposite direction for miR-21 recognition. Introduction of miR-21 can hybridize either with Track 1 or with Track 2 to activate the BAS-DNA machine with a ...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Lan Lin Yingying Zhang Qiongying Wei Hongguang Lin Xiaoping Li Mei-E Yu Jie Wang Zhenghui Huang Dan Xue Source Type: research

Assays to Estimate the Binding Affinity of Aptamers
Talanta. 2022 Feb 1;238(Pt 1):122971. doi: 10.1016/j.talanta.2021.122971. Epub 2021 Oct 14.ABSTRACTAptamers have become coming-of-age molecular recognition elements in both diagnostic and therapeutic applications. Generated by SELEX, the 'quality control' of aptamers, which involves the validation of their binding affinity against their respective targets is pivotal to ascertain their potency prior to use in any downstream assays or applications. Several aptamers have been isolated thus far, however, the usage of inappropriate validation assays renders some of these aptamers dubitable in terms of their binding capabilities...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ramesh Thevendran Marimuthu Citartan Source Type: research

Development of an analytical method to detect simultaneously 219 new psychoactive substances and 65 other substances in urine specimens using LC-QqQ MS/MS with CriticalPairFinder and TransitionFinder
Talanta. 2022 Feb 1;238(Pt 1):122979. doi: 10.1016/j.talanta.2021.122979. Epub 2021 Oct 21.ABSTRACTEmerging new psychoactive substances (NPS) poses a great risk to public health. Analyzing these large numbers of NPS and other associated substances often relies on liquid chromatography coupled to triple quadrupole mass spectrometry (LC-QqQ-MS) with multiple-reaction monitoring (MRM) mode. However, the differentiation of critical pairs, coeluted isobaric and/or isomeric species, is one of the challenges for this analytical platform. MRM transitions with poor selectivity can jeopardize accurate quantification and lead to bias...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ju-Yu Chen Guan-Yuan Chen San-Yuan Wang Cheng-Chung Fang Lian-Yu Chen Te-I Weng Source Type: research

A gas chromatography-mass spectrometry method for quantifying squalane and squalene in aerosol emissions of electronic cigarette, or vaping, products
Talanta. 2022 Feb 1;238(Pt 1):122985. doi: 10.1016/j.talanta.2021.122985. Epub 2021 Oct 21.ABSTRACTNumerous chemicals of unknown inhalational toxicity have been measured in electronic cigarette, or vaping, products (EVPs). In addition, little is known about the liquid-to-aerosol transmission and deliveries of these chemicals, including oil-like terpenes such as squalene (SQE) and squalane (SQA). To provide information on the aerosol deliveries of these compounds from EVPs, we developed and validated a quantitative method to measure squalene and squalane in EVP aerosol emissions. Validation parameters include measurement re...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Elizabeth A Cowan Hang Tran Naudia Gray Jose J Perez Clifford Watson Benjamin C Blount Liza Valent ín-Blasini Source Type: research

Sensitive electrochemical immunosensor for CYFRA21-1 detection based on AuNPs@MoS < sub > 2 < /sub > @Ti < sub > 3 < /sub > C < sub > 2 < /sub > T < sub > x < /sub > composites
Talanta. 2022 Feb 1;238(Pt 1):122987. doi: 10.1016/j.talanta.2021.122987. Epub 2021 Oct 21.ABSTRACTCytokeratin fragment antigen 21-1 (CYFRA21-1) is a sensitive marker for detecting non-small cell lung cancer (NSCLC). Ti3C2Tx modified by gold nanoparticles (AuNPs) and molybdenum disulfide (MoS2) were synthesized for the first time to obtain the AuNPs@MoS2@Ti3C2Tx composites, which have large specific surface area and good electrocatalytic properties. A novel electrochemical immunoassay for sensitive detection of CYFRA21-1 was developed by loading a large quantity of secondary antibodies (Ab2) and toluidine blue (TB) on the ...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Kai Hu Jiamin Cheng Kangbin Wang Yuanqing Zhao Yanju Liu Huaixia Yang Zhenqiang Zhang Source Type: research

Si@Ag@PEI substrate-based SERS sensor for rapid detection of illegally adulterated sulfur dioxide in traditional Chinese medicine
Talanta. 2022 Feb 1;238(Pt 1):122988. doi: 10.1016/j.talanta.2021.122988. Epub 2021 Oct 22.ABSTRACTThe illegal adulteration of sulfur dioxide in natural healthcare products may lead to serious health problems, which raise an urgent demand of straightforward approach for detecting sulfur dioxide. In this paper, surface-enhanced Raman scattering (SERS) sensor with sample preparation apparatus for headspace adsorption of SO2 has been developed, which was successfully applied to detect illegal adulteration of sulfur dioxide in traditional Chinese medicine (TCM). Functional membrane substrate of Si@Ag@PEI composite was synthesi...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Bo Fan Yingchao Wang Zhenhao Li Dejin Xun Jian Dong Xiangwei Zhao Xiaohui Fan Yi Wang Source Type: research

Electrochemical determination of nitroaromatic explosives using glassy carbon/multi walled carbon nanotube/polyethyleneimine electrode coated with gold nanoparticles
Talanta. 2022 Feb 1;238(Pt 1):122990. doi: 10.1016/j.talanta.2021.122990. Epub 2021 Oct 22.ABSTRACTThe on site/in field detection of explosives has become a rising priority for homeland security and counter-terrorism measures. This work presents the sensitive detection of nitroaromatic explosives using glassy carbon/multi-walled carbon nanotubes/polyethyleneimine (GC/MWCNTs/PEI) electrode coated with gold nanoparticles (AuNPs). MWCNTs and PEI could be well dispersed in ethanol/water solution, giving rise to a thin and homogeneous film on GCE. The GC/MWCNTs/PEI electrode was electrochemically modified with AuNPs and used fo...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Aysu Arman Şener Sağlam Ay şem Üzer Re şat Apak Source Type: research

3D carbonized wood-based integrated electrochemical immunosensor for ultrasensitive detection of procalcitonin antigen
Talanta. 2022 Feb 1;238(Pt 1):122991. doi: 10.1016/j.talanta.2021.122991. Epub 2021 Oct 22.ABSTRACTThis work presents a novel 3D carbonized wood-based integrated electrochemical immunosensor for ultrasensitive detection of procalcitonin (PCT) antigen at picogram level, achieving a wide linear detection range for PCT concentrations range from 0.05 to 90 pg mL-1 with a low detection limit of 0.014 pg mL-1 (S/N = 3), outperforming the previous reports. 3D carbonized wood as a new immunosensor matrix is used for electrochemical PCT biosensing, improving the stability of electrode and overcoming the disadvantages of traditional...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Jianzhi Huang Wenxue Cheng Yanliang Li Source Type: research

PEGylation of boronate-affinity-oriented surface imprinting magnetic nanoparticles with improved performance
Talanta. 2022 Feb 1;238(Pt 1):122992. doi: 10.1016/j.talanta.2021.122992. Epub 2021 Oct 22.ABSTRACTHigh specific selectivity is the continuous goal of exploit glycoprotein-imprinted materials. Boronate-affinity-oriented surface imprinting can limit the heterogeneity of imprinted cavities, and PEGylation can reduce the nonspecific adsorption of imprinted materials towards non-target molecules. However, there are no reports on the integration of the above two advantages. Herein, we first integrated the boronate-affinity-oriented surface imprinting and PEGylation, and fabricated PEGylated boronate-affinity-oriented surface im...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Yi Luo Chen-Chen Bai Ming-Xia Liu Di Wang Meng-Ying Chen Shi-Song Yu Xin-Ying Bu Xian-Hua Wang Source Type: research

Detection of nitric oxide radical and determination of its scavenging activity by antioxidants using spectrophotometric and spectrofluorometric methods
Talanta. 2022 Feb 1;238(Pt 1):122993. doi: 10.1016/j.talanta.2021.122993. Epub 2021 Oct 22.ABSTRACTAlthough reactive nitrogen species (RNS) may attack biomacromolecules and cause tissue damage when unbalanced by natural antioxidant defenses of the organism, they can also take part in cell signaling under different physiological states and defend against certain pathogens. Since there is a scarcity of analytical methods to detect radicalic NO and its scavengers, a functionalized gold nanoparticle-based spectrophotometric method and a spectrofluorometric method have been separately developed to test antioxidant activity towa...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ziya Can B üşra Keskin Ay şem Üzer Re şat Apak Source Type: research

Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area
Talanta. 2022 Feb 1;238(Pt 1):122994. doi: 10.1016/j.talanta.2021.122994. Epub 2021 Nov 3.ABSTRACTWe developed a small fluorescence microplate reader with an organic photodiode (OPD) array. The OPD array has nine OPDs that have a large light receiving area (9.62 mm2 per one OPD). Since the OPD array is fabricated on a flat glass plate, it can be placed just below microwells and can detect fluorescence emitted through the entire surface of the microwell bottom. The analytical performance of the developed plate reader was evaluated by measuring an aqueous solution of resorufin. The limit of detection (LOD) for resorufin (0.0...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Kazuhiro Morioka Moeko Osashima Nao Azuma Kuizhi Qu Akihide Hemmi Atsushi Shoji Hiroya Murakami Norio Teshima Tomonari Umemura Katsumi Uchiyama Hizuru Nakajima Source Type: research

Homing peptide combined with DNAzyme-based ELISA-like assay for highly specific and sensitive detection of fibrin
Talanta. 2022 Feb 1;238(Pt 1):122995. doi: 10.1016/j.talanta.2021.122995. Epub 2021 Oct 28.ABSTRACTA highly sensitive and specific ELISA-like chemiluminescence method for detection of fibrin has been developed. In the sensing platform, the homing peptide (CREKA), as recognition molecule, which can specially recognize the fibrin on microtiter plate, combined with G-quadruplex-based DNAzyme to form the probe of G-quadruplex-hemin DNAzyme-CREKA. After the sample solution was coated on the plates, the probe was crosslinked with fibrin through the interaction of CREKA and fibrin. Finally, luminol-H2O2 chemiluminesecence (CL) re...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Xifang Zhang Jinrong Zhang Yuanfu Zhang Yinghong Zhang Tingting Hou Shuhao Wang Source Type: research

Amperometric biosensors for L-arginine and creatinine assay based on recombinant deiminases and ammonium-sensitive Cu/Zn(Hg)S nanoparticles
Talanta. 2022 Feb 1;238(Pt 1):122996. doi: 10.1016/j.talanta.2021.122996. Epub 2021 Oct 22.ABSTRACTThere are limited data on amperometric biosensors (ABSs) based on deiminases that produce ammonium as a byproduct of enzymatic reaction. The most frequently proposed biosensors utilizing such a mode are based on potentiometric transducers, which contain at least two enzymes in the bioselective layer; this complicates the procedure and increases the cost of analysis. Thus, the construction of a one-enzyme ABS is a practical problem. In our manuscript ABSs for the direct measurement of creatinine (Crn) and l-arginine (Arg), bas...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: N Ye Stasyuk G Z Gayda A E Zakalskiy L R Fayura O M Zakalska А А Sibirny M Nisnevitch M V Gonchar Source Type: research

The critical evaluation of the effects of imidazolium-based ionic liquids on the separation efficiency of selected biogenic amines and their metabolites during MEKC analysis
This study uses micellar electrokinetic chromatography (MEKC) to evaluate how ILs in the background electrolyte (BGE) affect the separation efficiency of biogenic amines (BAs). Specifically, this research focuses on 12 ILs built from cations containing an imidazole ring with different alkyl substituents and anions, as well as one IL containing a pyridinium cation with tetrafluoroborate anion. All analyzed ILs, which were added to the BGE in concentrations ranging from 1 to 20 mM, were tested for their ability to improve the electrophoretic separation of selected BAs, namely: homovanillic acid (HVA), vanililmandelic acid (V...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Natalia Kaczmarczyk Julita Ci żewska Natalia Treder Natalia Mi ękus Alina Plenis Piotr Kowalski Anna Roszkowska Tomasz B ączek Ilona Ol ędzka Source Type: research

Evaluation of dried matrix spot sampling for total sulphur determination in automotive gasoline by high-resolution continuum source molecular absorption spectrometry and direct solid sample analysis
Talanta. 2022 Feb 1;238(Pt 1):122998. doi: 10.1016/j.talanta.2021.122998. Epub 2021 Oct 29.ABSTRACTIn this work, a method for total sulphur determination in automotive gasoline using dried matrix spot sampling is proposed. The method is based on the deposition of the sample on a cellulose-based filter paper and subsequent sulphur quantification via CS diatomic molecule using high-resolution continuum source graphite furnace molecular absorption spectrometry (HR-CS GF MAS). The sample deposition was carried out, along with the chemical modifier, on a 20-mm filter paper disc previously adapted into a polytetrafluoroethylene ...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Leandro Kolling Morgana Bazzan Dessuy Maria Goreti Rodrigues Vale M árcia Messias da Silva Source Type: research

Tyndall-Effect-inspired assay with gold nanoparticles for the colorimetric discrimination and quantification of mercury ions and glutathione
Talanta. 2022 Feb 1;238(Pt 1):122999. doi: 10.1016/j.talanta.2021.122999. Epub 2021 Oct 29.ABSTRACTThis work initially reports a new nanosening method for simple, sensitive, specific, visual detection of mercury (II) (Hg2+) and glutathione (GSH) using the Tyndall Effect (TE) of the same colloidal gold nanoparticle (GNP) probes for efficient colorimetric signaling amplification. For the TE-inspired assay (TEA) method, arginine (Arg) molecules are pre-modified on the GNPs' surfaces (Arg-GNPs). Upon the Hg2+ introduction, it can be specifically coordinated with the terminal -NH2 and -COOH groups of the Arg molecules to make t...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Yao Sun Kaijing Yuan Xiaomei Mo Xuejiang Chen Yanan Deng Chang Liu Yali Yuan Jinfang Nie Yun Zhang Source Type: research

Ultrasensitive Pd nano catalyst as peroxidase mimetics for colorimetric sensing and evaluation of antioxidants and total polyphenols in beverages and fruit juices
Talanta. 2022 Feb 1;238(Pt 1):123000. doi: 10.1016/j.talanta.2021.123000. Epub 2021 Oct 27.ABSTRACTHerein, we developed a new Pd NP from the aq extract of Elsholtzia blanda Benth. flower that showed efficient peroxidase mimetic activity. The catalytic mechanism was confirmed through colorimetric analysis. The optimizations of temperature, concentration, PH and time were done to find out the best procedure to implement the intrinsic catalytic activity in practical applications. Michaelis-Menten constants were evaluated for both TMB and H2O2 substrate to investigate the affinity of Pd NP towards them. Km was observed to be 4...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Nirangkush Borah Chandan Tamuly Source Type: research

Biomimetic-mineralized bifunctional nanoflowers for enzyme-free and colorimetric immunological detection of protein biomarker
Talanta. 2022 Feb 1;238(Pt 1):123001. doi: 10.1016/j.talanta.2021.123001. Epub 2021 Oct 28.ABSTRACTDetection of protein biomarkers relies largely on the development of modern immunological methods. Herein, a new enzyme-free immunological method is proposed to detect protein biomarkers. Employment of antibody-Cu3(PO4)2 hybrid nanoflowers, which are prepared through a facile and mild biomimetic-mineralizing process, is the core concept of the method. These nanoflowers can perform functions: one is to bind to target protein biomarkers with high specificity; the other is to release large amounts of Cu2+ upon acid treatment, wh...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Wenming Pan Tinwang Jiang Tong Lu Quanshan Jin Yanhai Xi Wenju Zhang Source Type: research

In-syringe dispersive liquid-liquid microextraction
Talanta. 2022 Feb 1;238(Pt 1):123002. doi: 10.1016/j.talanta.2021.123002. Epub 2021 Oct 28.ABSTRACTDispersive liquid-liquid microextraction (DLLME) has recently been widely used in the separation and preconcentration of various chemical species. Among the various approaches using DLLME are systems that use a syringe as an extraction environment. In this review, details of some methods that use this approach are presented. The ways to promote dispersion, analytical characteristics, and the advantages and disadvantages of the methods, among other aspects, are discussed critically. Finally, some trends in the use of in-syring...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Valfredo Azevedo Lemos Jeferson Alves Barreto Luana Bastos Santos Rosivan Dos Santos de Assis Cleber Galv ão Novaes Ricardo J Cassella Source Type: research

Rapid and multiplexed quantification of Salmonella, Escherichia coli O157:H7, and Shigella flexneri in ground beef using flow cytometry
In this study, a sample pretreatment protocol and a flow cytometry method were developed for rapid and multiplexed quantification of the three pathogens without cultural enrichment in ground beef. The whole process of sample pretreatment, staining, and instrument analysis can be accomplished within 1 h. The three bacteria upon sample pretreatment were demonstrated good recoveries (93.8%-101.2%). The quantitative detection range of the mothed was 103 to 108 cells/g for all three pathogens, and the detection limit for Salmonella, E. coli O157:H7 and S. flexneri in ground beef were 3.1 × 103 cells/g, 2.1 × 103 cel...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ziquan Wang Qian Xu Siyuan Liu Yingying Liu Ying Gao Meng Wang Ling Zhang Haiyan Chang Qiang Wei Zhiwei Sui Source Type: research

Nanozyme catalysis-assisted ratiometric multicolor sensing of heparin based on target-specific electrostatic-induced aggregation
Talanta. 2022 Feb 1;238(Pt 1):123003. doi: 10.1016/j.talanta.2021.123003. Epub 2021 Oct 28.ABSTRACTMonitoring the level of heparin in clinical matrices is significant because of its pivotal role in preventing thrombosis. Compared to traditional single-signal sensors, multi-signal ratiometric detection can provide anti-interference results especially in complicated environments. However, fabricating an easy-to-operation, low-cost and robust sensor for the ratiometric detection of heparin still remains challenging. Here we propose a novel nanosensor for the ratiometric multicolor sensing of heparin with high performance. The...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Xiangheng Niu Mengzhu Wang Hengjia Zhu Peng Liu Jianming Pan Bangxiang Liu Source Type: research

Liquid chromatography coupled to Venturi easy ambient sonic spray ionization mass spectrometry
Talanta. 2022 Feb 1;238(Pt 1):123004. doi: 10.1016/j.talanta.2021.123004. Epub 2021 Oct 30.ABSTRACTVenturi easy ambient sonic spray ionization (V-EASI) is a soft ambient ionization (AI) source that has the advantages of being suitable to the analysis of samples in solution (differently from the majority of AI sources), performing self-pumping, voltage- and heat-free ionization, and requiring minimum or no sample preparation. Since this ionization technique has not been fully explored, the present study provides a proof of principle of the coupling of liquid chromatography to mass spectrometry (LC-MS) using V-EASI as the in...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Luan Felipe Campos Oliveira David Ulisses Tega Marcos Nogueira Eberlin Alessandra Sussulini Source Type: research

Identification of glycerophospholipids using self-built recognition software based on positive and negative ion high-resolution mass spectrometric fragmentation experiments
Talanta. 2022 Feb 1;238(Pt 1):123006. doi: 10.1016/j.talanta.2021.123006. Epub 2021 Oct 29.ABSTRACTGlycerophospholipids (GPs) have a wide variety and complex structure, which makes their identification challenging. Our software affords a novel tool for the automated identification of non-target GPs in biological mixtures. Here, we explored the multi-stage fragmentation processes of GPs in positive and negative ion modes, and then constructed multi-stage fragment ion databases. This database includes 8214 simulated GP molecules from a random combination of fatty acids corresponding to 42,439 self-built predicted multi-stage...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ya Zhao Xinnan Zhao Tao Li Xiao Wang Cheng Zhong Xiupin Wang Peiwu Li Source Type: research

Visualization of peroxynitrite in cyclophosphamide-induced oxidative stress by an activatable probe
Talanta. 2022 Feb 1;238(Pt 1):123007. doi: 10.1016/j.talanta.2021.123007. Epub 2021 Oct 29.ABSTRACTOxidative stress is considered to be one of the main contributors of cyclophosphamide (CP)-induced toxicity, and the generation of free radicals will cause the interruption of multiple signal transduction pathways. Peroxynitrite (ONOO-) has strong oxidation and nitrification ability and is considered as an indirect indicator of oxidative stress. Therefore, it is necessary to design a fluorescent probe that can detect ONOO- and monitor CP-induced oxidative stress during chemotherapy. Herein, we synthesized a lipid droplet targ...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Jinsa Li Shuxin Peng Zipeng Li Fangfang Zhao Xiaojing Han Jianfei Liu Wenbo Cao Yong Ye Source Type: research

Analysis of drugs including illicit and new psychoactive substances in oral fluids by gas chromatography-drift tube ion mobility spectrometry
In this study, a gas chromatograph (GC) has been coupled to a drift tube ion mobility spectrometer (IMS) in order to develop an analytical procedure for the determination of psychoactive substances in oral fluids. Working parameters, including the GC-IMS interface ones, were adjusted in order to obtain sensitive and robust signals. A volume of 500 μL of oral fluid was extracted with 250 μL chloroform and, after centrifugation, were injected into the GC-IMS system. Amphetamine, methylone, α-PVP, ketamine, lidocaine, MPHP, cocaine, THJ-2201, and 5F-ADB were employed as model compounds, providing limits of detecti...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Ariadna Denia Francesc A Esteve-Turrillas Sergio Armenta Source Type: research

Simultaneous quantification of selected glycosaminoglycans by butanolysis-based derivatization and LC-SRM/MS analysis for assessing glycocalyx disruption in vitro and in vivo
Talanta. 2022 Feb 1;238(Pt 1):123008. doi: 10.1016/j.talanta.2021.123008. Epub 2021 Oct 28.ABSTRACTGlycosaminoglycans (GAGs) constitute the main building blocks of the endothelial glycocalyx (GLX), and disruption of GLX initiates and promotes endothelial dysfunction. Here, we aimed to develop a novel, specific and accurate LC-SRM/MS-based method for glycosaminoglycans (GAGs) profiling. The method involved butanolysis derivatization to facilitate GAG-specific disaccharide generation and its subsequent retention in LC-reversed-phase mode followed by mass spectrometric detection performed in positive ion-selected reaction mon...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Karolina Matyjaszczyk-Gwarda Agnieszka Kij Mariola Olkowicz Benedikt Fels Kristina Kusche-Vihrog Maria Walczak Stefan Chlopicki Source Type: research

Selective sensing of the nucleoside analogue, trifluridine and tipiracil in dosage form and biological matrices
Talanta. 2022 Feb 1;238(Pt 1):123009. doi: 10.1016/j.talanta.2021.123009. Epub 2021 Oct 28.ABSTRACTA new fluorescent sensor is introduced to analyze nucleoside analogue, trifluridine and tipiracil in tablets and biological fluids. The synthesized fluorophore exhibits good fluorescence at 446 nm after excitation at 257 nm. The interaction between the studied drugs and the reagent was a quenching effect. Different experimental parameters and the mechanism of quenching were discussed. The present method was utilized to analyze trifluridine and tipiracil raw materials and tablets over the concentration range of 20-1000 ng/mL a...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Wael Talaat Mohamed M Y Kaddah Reda Mohammed Keshk Source Type: research

An experimental design approach for the optimization of scopoletin extraction from Morinda citrifolia L. using accelerated solvent extraction
Talanta. 2022 Feb 1;238(Pt 1):123010. doi: 10.1016/j.talanta.2021.123010. Epub 2021 Oct 29.ABSTRACTScopoletin is regarded as a major constituent of noni (Morinda citrifolia L), which contributes to the antioxidative, anti-inflammatory, immunomodulatory, and hepatoprotective properties in noni. It is also suggested as a marker for identification and quality control of noni and its derivative products. With the increasing interest in noni due to its health benefits and therapeutic effects, it is important to establish a reliable extraction and analysis method to determine scopoletin content in noni samples. The present study...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Aprilia Nur Tasfiyati Lucia Dwi Antika Rizna Triana Dewi Abdi Wira Septama Akhmad Sabarudin Teni Ernawati Source Type: research

High sensitive electrochemical methamphetamine detection in serum and urine via atom transfer radical polymerization signal amplification
Talanta. 2022 Feb 1;238(Pt 1):123026. doi: 10.1016/j.talanta.2021.123026. Epub 2021 Nov 3.ABSTRACTHerein we designed a highly sensitive and selective biosensor for methamphetamine (METH) detection based on aptamer recognition probe and atom transfer radical polymerization (ATRP) signal amplification strategy. In this experiment, METH aptamer and its complementary DNA strand were first attached to the electrode surface. In the presence of METH, the prioritized conjugation between METH and the aptamer will take one strand of DNA from the double-stranded DNA, so that the third segment of azide-modified DNA could be successful...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Haobo Sun Jingliang Liu Yunliang Qiu Jinming Kong Xueji Zhang Source Type: research

Three-dimensional macroscopic graphene supported vertically-ordered mesoporous silica-nanochannel film for direct and ultrasensitive detection of uric acid in serum
Talanta. 2022 Feb 1;238(Pt 1):123027. doi: 10.1016/j.talanta.2021.123027. Epub 2021 Nov 1.ABSTRACTDirect, rapid and sensitive detection of physiologically-relevant active small molecules (ASMs) in complex biological samples is highly desirable. Herein, we present an electrochemical sensing platform by combining three-dimensional macroscopic graphene (3DG) and vertically-ordered mesoporous silica-nanochannel film (VMSF), which is able to directly detect ASMs in complex samples with high sensitivity and no need of tedious pretreatment. Free-standing and macroscopic 3DG serves as the supporting electrode and O2-plasma treatme...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Xiaoqi Zhu Lingli Xuan Jiawei Gong Junjie Liu Xiaobo Wang Fengna Xi Jie Chen Source Type: research

Impact of gadolinium oxide with functionalized carbon nanosphere: A portable advanced electrocatalyst for pesticide detection in aqueous environmental samples
In this study, we developed a portable electrochemical sensor for realizing the pesticide residue in biological, environmental, and vegetable samples. A lower concentration of carbendazim pesticide (CBZ) was electrochemically exposed by newly developed gadolinium oxide/functionalized carbon nanosphere modified glassy carbon electrode (Gd2O3/f-CNS/GCE). The Gd2O3/f-CNS composite was prepared by two-pot ultrasonic-assisted co-precipitation method and characterized by various physicochemical analytical techniques. In addition, the electrocatalytic activity of the composite was investigated by cyclic voltammetry (CV) towards t...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Krishnapandi Alagumalai Ragurethinam Shanmugam Shen-Ming Chen Balamurugan Thirumalraj Ahmed S Haidyrah Chelladurai Karuppiah Source Type: research

A ratiometric fluorescence sensor for 5-hydroxymethylfurfural detection based on strand displacement reaction
Talanta. 2022 Feb 1;238(Pt 1):123029. doi: 10.1016/j.talanta.2021.123029. Epub 2021 Nov 3.ABSTRACTSince 5-hydroxymethylfurfural (HMF) becomes a neo-forming contaminant with latent harm to human health, development of new method for highly sensitive detection of HMF is extremely desirable. Herein, a novel ratiometric fluorescence sensor based on strand displacement reaction and magnetic separation was designed for sensitive and selective detection of HMF with the help of MnO2 nanosheets. The aldehyde-functionalized DNA (S0-CHO) and HMF competed for binding to amino-functionalized magnetic beads (NH2-MBs). Then, S0-CHO was c...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Nan Jiang Peng Li Shihao Sun Wei Wei Source Type: research

Evaluation of complexing agents in the gel electro-membrane extraction: An efficient approach for the quantification of zinc (II) ions in water samples
Talanta. 2022 Feb 1;238(Pt 1):123031. doi: 10.1016/j.talanta.2021.123031. Epub 2021 Nov 2.ABSTRACTIn this work, gel electro-membrane extraction (G-EME) combined with flame atomic absorption spectrometry (FAAS) was used for the determination of zinc ions (Zn2+) in water samples. For the first time, the effect of the presence of three types of complexing agents such as phenanthroline (Phen), crown ethers (12C4, 15C5, 18C6), and ethylene-diamine-tetra-acetic acid (EDTA) on the extraction efficiency of zinc ions was studied. In addition, the electroendosmosis (EEO) flow as an unwanted actuator was monitored in the presence and...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Somayeh Yousefi Somayeh Makarem Waleed Alahmad Farzaneh Dorabadi Zare Hadi Tabani Source Type: research

A label-free colorimetric aptasensor based on split aptamers-chitosan oligosaccharide-AuNPs nanocomposites for sensitive and selective detection of kanamycin
Talanta. 2022 Feb 1;238(Pt 1):123032. doi: 10.1016/j.talanta.2021.123032. Epub 2021 Nov 3.ABSTRACTHerein, the split aptamers, chitosan oligosaccharide, and AuNPs were combined as nanocomposites that present different formations to develop a label-free colorimetric aptasensor for rapid detection of small molecules. Kanamycin was chosen as a model target. Computational studies were performed to assist in the design of orientated immobilization of the split aptamers onto the AuNPs surface. Chitosan oligosaccharide was initially applied as an aggregation inducer of AuNPs, and chitopentaose was screened as the optimal. Under op...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Xiaoyan Qi Yinglin Zhao Haipeng Su Lele Wang Ling Li Rui Ma Xiaochen Yan Jianan Sun Sai Wang Xiangzhao Mao Source Type: research

Characterization of AgNPs and AuNPs in sewage sludge by single particle inductively coupled plasma-mass spectrometry
This study develops for the first time an analytical method for the characterization of silver and gold nanoparticles in sewage sludge. The evaluation of the effect of temperature, extracting agent and centrifugation speed and time on the extraction yield was carried out through a multifactorial analysis of variance which allows us to select 289 g, 5 min and 20 mM sodium pyrophosphate tetrabasic as optimal extraction conditions. Under these conditions, the analysis of the extract by single particle inductively coupled plasma-mass spectrometry provided recovery percentages of 70 ± 2% and 56 ± 1% for silver and...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Gustavo Moreno-Mart ín Beatriz G ómez-Gómez Maria Eugenia Le ón-González Yolanda Madrid Source Type: research

Ultrasensitive early detection of insulin antibody employing novel electrochemical nano-biosensor based on controllable electro-fabrication process
Talanta. 2022 Feb 1;238(Pt 1):122947. doi: 10.1016/j.talanta.2021.122947. Epub 2021 Oct 14.ABSTRACTAn ultrasensitive novel electrochemical nano-biosensor for rapid detection of insulin antibodies against diabetes antigens was developed in this research. The presence of insulin antibodies has been demonstrated to be a strong predictor for the development of type 1 diabetes in individuals who do not have diabetes but are genetically predisposed. The proposed nano-biosensor fabrication process was based on the optimized sequential electropolymerization of polyaniline and electrodeposition of gold nanoparticles on the surface ...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Mohammadreza Farrokhnia Ghassem Amoabediny Mohammad Ebrahimi Mohammadreza Ganjali Mohammad Arjmand Source Type: research

Development of QDs-based nanosensors for heavy metal detection: A review on transducer principles and in-situ detection
Talanta. 2021 Sep 23:122903. doi: 10.1016/j.talanta.2021.122903. Online ahead of print.ABSTRACTHeavy metal pollution has severe threats to the ecological environment and human health. Thus, it is urgent to achieve the rapid, selective, sensitive and portable detection of heavy metal ions. To overcome the defects of traditional methods such as time-consuming, low sensitivity, high cost and complicated operation, QDs (Quantum dots)-based nanomaterials have been used in sensors to significantly improve the sensing performance. Due to their excellent physicochemical properties, high specific surface area, high adsorption and r...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Xinyi Wang Liubing Kong Shuqi Zhou Chiyu Ma Wencheng Lin Xianyou Sun Dmitry Kirsanov Andrey Legin Hao Wan Ping Wang Source Type: research

An aggregation-induced emission fluorescence probe for evaluating the effect of CYP450 changes under tumor chemotherapy
This study presented that CYP450 level in HepG2/DDP cells (cisplatin-resistant cells) was higher than that in HepG2 cells, and the inhibition of CYP450 by 1-ABT effectively improved the tumor resistance. Thus, CYP450 plays a key role in the development of tumor resistance. The synergistic effect of 1-ABT and the chemotherapeutic agent cisplatin was superior to that of cisplatin alone in tumor-bearing mice. The TPE-CYP probe will provide an idea for the clinical implementation of individualized tumor treatment strategies, through the accurate monitoring of CYP450.PMID:34861484 | DOI:10.1016/j.talanta.2021.123111 (Source: Talanta)
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Pengpeng Lu Yan Huang Caiyun Zhang Lili Fu Xiaoyan Wang Lingxin Chen Source Type: research

Disposable biosensor based on novel ternary Ru-PEI@PCN-333(Al) self-enhanced electrochemiluminescence system for on-site determination of caspase-3 activity
This study lays a foundation for the development of more advanced, ingenious, portable and reliable ECL devices for biosensing not only caspase-3, but also other bioanalytes.PMID:34861485 | DOI:10.1016/j.talanta.2021.123083 (Source: Talanta)
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Weiwei Luo Hongyu Chu Xinzhao Wu Pinyi Ma Qiong Wu Daqian Song Source Type: research

A novel turn-on type AIE fluorescent probe for highly selective detection of cysteine/homocysteine and its application in living cells
Talanta. 2021 Nov 23;239:123091. doi: 10.1016/j.talanta.2021.123091. Online ahead of print.ABSTRACTBiothiols, associated with multiple physiological and pathological processes, have structural similarities. Monitoring Biothiols selectively in organisms is of great significance. Burdened by the aggregation-caused quenching (ACQ) effect, the applications of conventional biothiols fluorescent probes are extremely limited. Herein, we developed a "turn-on" type aggregation-induced emission (AIE) fluorescent probe BQM-NBD, which was composed of a BQM-OH fluorophore molecule with AIE effect and the recognition group 7-n...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Wei Wang Min Ji Junqing Chen Peng Wang Source Type: research

Protein biosensor based on Schottky barrier nanowire field effect transistor
Talanta. 2021 Nov 24;239:123092. doi: 10.1016/j.talanta.2021.123092. Online ahead of print.ABSTRACTA top-down nanofabrication approach involving molecular beam epitaxy and electron beam lithography was used to obtain silicon nanowire-based back gate field-effect transistors with Schottky contacts on silicon-on-insulator (SOI) wafers. The resulting device is applied in biomolecular detection based on the changes in the drain-source current (IDS). In this context, we have explained the physical mechanisms of charge carrier transport in the nanowire using energy band diagrams and numerical 2D simulations in TCAD. The results ...
Source: Talanta - December 2, 2021 Category: Chemistry Authors: Tatyana E Smolyarova Lev V Shanidze Anna V Lukyanenko Filipp A Baron Vasilisa V Krasitskaya Anna S Kichkailo Anton S Tarasov Nikita Volkov Source Type: research

Protein biosensor based on Schottky barrier nanowire field effect transistor
Talanta. 2021 Nov 24;239:123092. doi: 10.1016/j.talanta.2021.123092. Online ahead of print.ABSTRACTA top-down nanofabrication approach involving molecular beam epitaxy and electron beam lithography was used to obtain silicon nanowire-based back gate field-effect transistors with Schottky contacts on silicon-on-insulator (SOI) wafers. The resulting device is applied in biomolecular detection based on the changes in the drain-source current (IDS). In this context, we have explained the physical mechanisms of charge carrier transport in the nanowire using energy band diagrams and numerical 2D simulations in TCAD. The results ...
Source: Talanta - December 2, 2021 Category: Chemistry Authors: Tatyana E Smolyarova Lev V Shanidze Anna V Lukyanenko Filipp A Baron Vasilisa V Krasitskaya Anna S Kichkailo Anton S Tarasov Nikita Volkov Source Type: research

Single-particle analysis of tear fluid reveals abundant presence of tissue factor-exposing extracellular vesicles with strong coagulation activity
Talanta. 2021 Nov 25;239:123089. doi: 10.1016/j.talanta.2021.123089. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) in easily accessible body fluids have emerged as a promising source for liquid biopsy. Although tear collection is fast, safe, and noninvasive, EVs of tear fluid are less studied and their involvement in physiological and pathological processes is largely unknown. The aim of present study was to analyze and characterize EVs in tear fluid at the single-particle level to reveal the population heterogeneity. A laboratory-built nano-flow cytometer (nFCM) was used to analyze the purity, size distributi...
Source: Talanta - November 30, 2021 Category: Chemistry Authors: Haisheng Liu Wenli Yuan Qisheng Pang Chengfeng Xue Xiaomei Yan Source Type: research

Single-particle analysis of tear fluid reveals abundant presence of tissue factor-exposing extracellular vesicles with strong coagulation activity
Talanta. 2021 Nov 25;239:123089. doi: 10.1016/j.talanta.2021.123089. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) in easily accessible body fluids have emerged as a promising source for liquid biopsy. Although tear collection is fast, safe, and noninvasive, EVs of tear fluid are less studied and their involvement in physiological and pathological processes is largely unknown. The aim of present study was to analyze and characterize EVs in tear fluid at the single-particle level to reveal the population heterogeneity. A laboratory-built nano-flow cytometer (nFCM) was used to analyze the purity, size distributi...
Source: Talanta - November 30, 2021 Category: Chemistry Authors: Haisheng Liu Wenli Yuan Qisheng Pang Chengfeng Xue Xiaomei Yan Source Type: research

Measurement methods of single cell drug response
Talanta. 2021 Nov 3:123035. doi: 10.1016/j.talanta.2021.123035. Online ahead of print.ABSTRACTIn the last decades, a wide multitude of research activity has been focused on the development of new drugs, and devoted to overcome the challenges of high cost and low efficiency in drug evaluation. The measurement of drug response at the single cell level is a quicker, more direct and more accurate way to reflect drug efficacy, which can shorten the drug development period and reduce research costs. Therefore, the single cell drug response (SCDR) measurement technology has aroused extensive attention from researchers, and has be...
Source: Talanta - November 29, 2021 Category: Chemistry Authors: Yuliang Zhao Jiazhi Lou Hongyu Zhang Hui Sun Menglin Zhang Shuyu Wang Xiaopeng Sha Zhikun Zhan Ying Wang Cuihua Ma Wen Jung Li Source Type: research

N-acetyl- β-d-glucosaminidase activity assay for monitoring insulin-dependent diabetes using Ag-porous Si SERS platform
Talanta. 2021 Nov 22:123087. doi: 10.1016/j.talanta.2021.123087. Online ahead of print.ABSTRACTDetermination of urinary or serum N-acetyl-β-d-glucosaminidase (NAG) activity as a tissue damage indicator is widely used in diagnosis of various pathologies, including diabetic nephropathy. Early and rapid biomarker detection is an important element of medical diagnosis, facilitating prompt therapeutic decisions and prognosis evaluation. Herein, we present a modified sensing approach for a rapid and reliable NAG activity determination in complex media using surface-enhanced Raman spectroscopy (SERS). Porous silicon (PSi) Fa...
Source: Talanta - November 29, 2021 Category: Chemistry Authors: Narsingh R Nirala Jimmy Asiku Hay Dvir Giorgi Shtenberg Source Type: research

Measurement methods of single cell drug response
Talanta. 2021 Nov 3:123035. doi: 10.1016/j.talanta.2021.123035. Online ahead of print.ABSTRACTIn the last decades, a wide multitude of research activity has been focused on the development of new drugs, and devoted to overcome the challenges of high cost and low efficiency in drug evaluation. The measurement of drug response at the single cell level is a quicker, more direct and more accurate way to reflect drug efficacy, which can shorten the drug development period and reduce research costs. Therefore, the single cell drug response (SCDR) measurement technology has aroused extensive attention from researchers, and has be...
Source: Talanta - November 29, 2021 Category: Chemistry Authors: Yuliang Zhao Jiazhi Lou Hongyu Zhang Hui Sun Menglin Zhang Shuyu Wang Xiaopeng Sha Zhikun Zhan Ying Wang Cuihua Ma Wen Jung Li Source Type: research

N-acetyl- β-d-glucosaminidase activity assay for monitoring insulin-dependent diabetes using Ag-porous Si SERS platform
Talanta. 2021 Nov 22:123087. doi: 10.1016/j.talanta.2021.123087. Online ahead of print.ABSTRACTDetermination of urinary or serum N-acetyl-β-d-glucosaminidase (NAG) activity as a tissue damage indicator is widely used in diagnosis of various pathologies, including diabetic nephropathy. Early and rapid biomarker detection is an important element of medical diagnosis, facilitating prompt therapeutic decisions and prognosis evaluation. Herein, we present a modified sensing approach for a rapid and reliable NAG activity determination in complex media using surface-enhanced Raman spectroscopy (SERS). Porous silicon (PSi) Fa...
Source: Talanta - November 29, 2021 Category: Chemistry Authors: Narsingh R Nirala Jimmy Asiku Hay Dvir Giorgi Shtenberg Source Type: research

One-pot synthesis of a peroxidase-like nanozyme and its application in visual assay for tyrosinase activity
Talanta. 2021 Nov 23:123088. doi: 10.1016/j.talanta.2021.123088. Online ahead of print.ABSTRACTBoth single-atom nanozymes (SAzymes) and protein-template metal nanoparticles have attracted comprehensive attention in several respects owing to their excellent catalytic performance, green facile synthesis process, and robustness. Herein, the peroxidase-like activity of single-atom copper anchored on bovine hemoglobin-template gadolinium nanoparticles (Cu,Gd@BHbFITC NPs) were successfully synthesized and two sensitive turn-on fluorescence strategies for tyrosinase (TYR) activity sensing were proposed for the first time. For str...
Source: Talanta - November 28, 2021 Category: Chemistry Authors: Shuangqin Li Di Liu Bingyan Wu Huipeng Sun Xiaoyan Liu Haixia Zhang Nana Ding Lan Wu Source Type: research