Simultaneous determination of short-chain fatty alcohols in aged oil and biodiesels by stable isotope labeling assisted liquid chromatography-mass spectrometry
Talanta. 2021 Jul 1;229:122223. doi: 10.1016/j.talanta.2021.122223. Epub 2021 Mar 9.ABSTRACTShort-chain fatty alcohols (SCFAs) are one of the reactants for the production of biodiesels. The SCFA residues at the trace level have a significant impact on biodiesel quality. However, the analysis of SCFAs in aged biodiesels has not been reported so far, which is probably due to the unavailability of an appropriate analytical method for the simultaneous determination of SCFAs. Herein, we developed a novel analytical approach with high sensitivity and selectivity for the simultaneous identification and determination of SCFAs in s...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Hua-Ming Xiao Shuai Zhao Ru-Ting Fan Dilshad Hussain Xian Wang Source Type: research

Chemometric pre-processing can negatively affect the performance of near-infrared spectroscopy models for fruit quality prediction
The objectives of this study are to test two hypotheses to explore the effect of pre-processing on NIR spectra of fresh fruit. The first hypothesis is that the pre-processing of NIR spectra with scatter correction techniques can reduce the predictive performance of models as the scatter correction can reduce the useful scattering information correlated to the property of interest. The second hypothesis is that the Deep Learning (DL) can model the raw absorbance data (mix of scattering and absorption) much more efficiently than the Partial Least Squares (PLS) regression analysis. To test the hypotheses, a real NIR data set ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Puneet Mishra Douglas N Rutledge Jean-Michel Roger Khan Wali Haris Ahmad Khan Source Type: research

A review on analytical methods with special reference to electroanalytical methods for the determination of some anticancer drugs in pharmaceutical and biological samples
Talanta. 2021 Jul 1;229:122247. doi: 10.1016/j.talanta.2021.122247. Epub 2021 Mar 7.ABSTRACTIt is widely accepted that cancer, the second leading cause of death, is a morbidity with big impacts on the global health. In the last few years, chemo-therapeutic treatment continually induces alone most lengthy consequents, which is extremely harmful for the physiological and psychological health of the patients. In the present research, we discuss the recent techniques for employed for extraction, and quantitative determination of such compounds in pharmaceutical, and biological specimens. In the frame of this information, this ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mohadeseh Safaei Masoud Reza Shishehbore Source Type: research

Off-flavor in soy drink: Development, optimization, and validation of an easy and fast method to quantify the key odorants
Talanta. 2021 Jul 1;229:122251. doi: 10.1016/j.talanta.2021.122251. Epub 2021 Mar 13.ABSTRACTA detailed molecular flavor profile was necessary to understand the low acceptance of soy drink by Western consumers. Accordingly, key odor-active compounds were detected by means of aroma dilution analyses coupled with gas chromatography-mass spectrometry-olfactometry after application of suitable solvent-free volatile extraction techniques. Four quantification methods (standard addition, external calibration, internal standard, and stable isotope dilution assay) were developed and validated to measure the concentrations after dir...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ann-Kathrin Nedele Nicole Mayer Natalie Feller J örg Hinrichs Yanyan Zhang Source Type: research

A simple, sensitive and efficient electrochemical platform based on carbon paste electrode modified with Fe < sub > 3 < /sub > O < sub > 4 < /sub > @MIP and graphene oxide for folic acid determination in different matrices
Talanta. 2021 Jul 1;229:122258. doi: 10.1016/j.talanta.2021.122258. Epub 2021 Mar 15.ABSTRACTA new biomimetic sensor selective to folic acid based on a carbon paste modified with graphene oxide and Fe3O4 nanoparticles coated with molecularly imprinted polymer in the core@shell format (Fe3O4@MIPs) was obtained using the polyol method. The sensing phase was synthesized in a simple way and characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Brunauer-Emmett-Teller (BET) surface area. In the binding experiments the selective material showed a high adsorption capacity (Q) of 30...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Sara Manente Garcia Ademar Wong Sabir Khan Maria D P T Sotomayor Source Type: research

Characterization of stem cell-like property in cancer cells based on single-cell impedance measurement in a microfluidic platform
Talanta. 2021 Jul 1;229:122259. doi: 10.1016/j.talanta.2021.122259. Epub 2021 Mar 9.ABSTRACTInvestigation of stem cell-like property in cancer cells is important for the development of new therapeutic drugs targeting at malignant tumors. Currently, the standard approach for identifying cancer stem cell-like cells relies on the recognition of stem cell surface markers. However, the reliability remains controversial among biologists. In the current work, a dielectrophoretic and impedimetric hybrid microfluidic platform was developed for capturing single cells and characterizing their stem cell-like property. Single cells wer...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Kin Fong Lei Yu-Chen Ho Chia-Hao Huang Chun-Hao Huang Ping Ching Pai Source Type: research

Voltammetry of 7-dehydrocholesterol as a new and useful tool for Smith-Lemli-Opitz syndrome diagnosis
This study shows for the first time that electrochemical oxidation of 7-dehydrocholesterol can be used for its voltammetric determination. Two classes of supporting electrolytes in acetonitrile and a mixture of acetonitrile-water were used: inorganic acids known to promote structural changes of steroids and indifferent electrolytes. Oxidation of 7-dehydrocholesterol at ca +0.8 V (vs. Ag/AgNO3 in acetonitrile) in 0.1 mol L-1 NaClO4 in acetonitrile is useful for its voltammetric detection using common bare electrode materials. Detection limits for 7-dehydrocholesterol lie in the low micromolar range for all the working elect...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Jan Klouda Lenka Bene šová Pavel Ko čovský Karolina Schwarzov á-Pecková Source Type: research

An improved strategy for analysis of lipid molecules utilising a reversed phase C < sub > 30 < /sub > UHPLC column and scheduled MS/MS acquisition
Talanta. 2021 Jul 1;229:122262. doi: 10.1016/j.talanta.2021.122262. Epub 2021 Mar 17.ABSTRACTMeasuring physiochemically diverse molecules (including lipids) which vary significantly in their concentrations poses a great analytical challenge. In untargeted lipidomics studies, reversed phase chromatography coupled with data-dependent MS/MS acquisition (DDA) is frequently applied. The optimal assay should deliver a high number of detected compounds with associated fragmentation data. In this work, we introduce novel 30 and 50 min UHPLC assays utilising lipid separation on a C30 stationary phase with a modified DDA strategy us...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Andris Jankevics Amelia Jenkins Warwick B Dunn Luk áš Najdekr Source Type: research

A novel fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH < sub > 2 < /sub > for low background and high sensitivity determination of H < sub > 2 < /sub > S
Talanta. 2021 Jul 1;229:122271. doi: 10.1016/j.talanta.2021.122271. Epub 2021 Mar 6.ABSTRACTHydrogen sulfide is typical metabolic marker and environmental pollutant which is worthwhile to determine. Herein, a low background and high sensitivity fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH2 is proposed for the determination of hydrogen sulfide. Firstly, a functional monomer 3,5-diaminobenzoic acid is employed to modify on the CAU-10-NH2, the product CAU-10-NH-dAba has strong fluorescent performance at 412 nm under an excitation wavelength of 320 nm. Subsequently, it is fur...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Wenpeng Gong Li Zhang Ying Yu Bixia Lin Yumin Wang Manli Guo Yujuan Cao Source Type: research

Sensitive detection of transcription factor by coupled fluorescence-encoded microsphere with exonuclease protection
Talanta. 2021 Jul 1;229:122272. doi: 10.1016/j.talanta.2021.122272. Epub 2021 Mar 3.ABSTRACTAberrant transcription factors (TFs) activities are closely related to the occurrence and development of various diseases. Herein, we presented a fluorescence-encoded microsphere-based approach for TFs detection coupling with common DNA footprinting assay. Target TFs specifically bound the binding sites of double-stranded DNA (dsDNA) probes which were conjugated to microspheres. Thus, the probes were protected from being hydrolyzed by exonuclease III (Exo III). Afterwards, biotins labeled on the probes reacted with streptavidin-phyc...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Yue Sun Liu Zang Choiwan Lau Xueji Zhang Jianzhong Lu Source Type: research

Application of magnetic nanomaterials in magnetic-chromatography: A review
Talanta. 2021 Jul 1;229:122273. doi: 10.1016/j.talanta.2021.122273. Epub 2021 Mar 9.ABSTRACTWith the advent of nanotechnology and its development, there have been dramatic advances in various aspects of diverse sciences. Nanotechnology encompasses the manipulating matter to create nanometre-scale materials with prodigious features and their implementation in a vast range of applications. The topic that is the current debate in today's scientific community and the transformation origin in modern technologies. Magnetic nanomaterials belong to the group of materials mainly consisting of a magnetic component, such as iron, and...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mahshid Manouchehri Shahram Seidi Fuad O Abdullah Source Type: research

Capture-SELEX for aptamer selection: A short review
Talanta. 2021 Jul 1;229:122274. doi: 10.1016/j.talanta.2021.122274. Epub 2021 Mar 5.ABSTRACTThe emerging aptamer, developed through the systematic evolution of ligands by exponential enrichment (SELEX) process, has revolutionized and facilitated the discoveries in basic research. Among all SELEX technology, Capture-SELEX is a variant of the in vitro selection process, which is suitable for isolating aptamers against small molecules. Capture-SELEX library was developed to enable the immobilization of the oligonucleotides instead of the target molecules during the aptamer selection process. The review provides an update on t...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Chen Lyu Imran Mahmood Khan Zhouping Wang Source Type: research

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites
Talanta. 2021 Jul 1;229:122275. doi: 10.1016/j.talanta.2021.122275. Epub 2021 Mar 15.ABSTRACTThere is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the d...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ayman Chmayssem Nicolas Verplanck Constantin Edi Tanase Guillaume Costa Karen Monsalve-Grijalba Simon Amigues M élanie Alias Maxime Gougis V éronique Mourier S éverine Vignoud Amir M Ghaemmaghami Pascal Mailley Source Type: research

A functional DNA-modified dual-response gold nanoprobe for simultaneously imaging the acidic microenvironment and membrane proteins of tumor cells
Talanta. 2021 Jul 1;229:122284. doi: 10.1016/j.talanta.2021.122284. Epub 2021 Mar 16.ABSTRACTTumor progression is a complicated process influenced by multiple factors, in which the acidic tumor microenvironment (TME) and altered tumor-associated membrane proteins (TA-MPs) are closely involved. Monitoring the status of these factors is of significance for tumor progression research. Here, we develop a novel probe for simultaneously imaging the acidic TME and TA-MPs in situ. In this probe, i-motif-forming sequences (strand I) are conjugated to a gold nanoparticle (AuNP) via gold-sulfur bonds for acid-response. Extended aptam...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ailing Kan Shuai Wang Liyan Zhang Xiaowen Xu Nan Zhang Wei Jiang Source Type: research

Application of magnetic nanomaterials in bioanalysis
Talanta. 2021 Jul 1;229:122285. doi: 10.1016/j.talanta.2021.122285. Epub 2021 Mar 12.ABSTRACTThe importance of magnetic nanomaterials and magnetic hybrid materials, which are classified as new generation materials, in analytical applications is increasingly understood, and research on the adaptation of these materials to analytical methods has gained momentum. Development of sample preparation techniques and sensor systems using magnetic nanomaterials for the analysis of inorganic, organic and biomolecules in biological samples, which are among the samples that analytical chemists work on most, are among the priority issue...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Erkan Yilmaz Gokhan Sarp Furkan Uzcan Ozgur Ozalp Mustafa Soylak Source Type: research

Toward a deeper and simpler understanding of serum protein-mediated transformations of magnetic nanoparticles by ICP-MS
Talanta. 2021 Jul 1;229:122287. doi: 10.1016/j.talanta.2021.122287. Epub 2021 Mar 11.ABSTRACTA great variety of magnetic nanomaterials are entering preclinical investigations with the objective to select the most promising candidates for diagnostic and therapeutic applications. For an analytical approach to be used as a high-throughput screening tool, simple and cost-efficient sample preparation protocol is a basiс prerequisite. Here, we demonstrate how the application of continuous magnetic field allows for quantitatively separating iron oxide magnetic nanoparticles from a mixture with human serum to facilitate monitorin...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Olga V Kuznetsova Maciej Jarosz Bernhard K Keppler Andrei R Timerbaev Source Type: research

Development of < sup > 148 < /sup > Gd analysis method using stable Gd
Talanta. 2021 Jul 1;229:122295. doi: 10.1016/j.talanta.2021.122295. Epub 2021 Mar 11.ABSTRACTThe analytical method of Gd determination was developed with the aim to analyse 148Gd in environmental and bioassay samples. It involves the use of anion exchange resin, extraction chromatography, and cation exchange resin. Alkaline fusion and calcium oxalate co-precipitation are used for solid samples dissolution and liquid samples preconcentration, respectively. Total method recovery was tested with natural Gd (157Gd) using ICP-QQQ-MS. A maximum total recovery of 75 % was obtained.PMID:33838781 | DOI:10.1016/j.talanta.2021.122295 (Source: Talanta)
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Grzegorz Olszewski Patric Lindahl Peter Frisk Mats Eriksson H åkan B L Pettersson Source Type: research

Recent advances and applications of cyclodextrins in magnetic solid phase extraction
Talanta. 2021 Jul 1;229:122296. doi: 10.1016/j.talanta.2021.122296. Epub 2021 Mar 17.ABSTRACTCyclodextrins (CDs) as a family of cyclic oligosaccharides are toroidal with a hydrophobic interior and a hydrophilic exterior. They are well-known for their ability to form host-guest inclusion complexes with different compounds. They are used as chiral stationary phases in high performance liquid chromatography (HPLC) and gas chromatography (GC) or as chiral reagents in the background electrolyte of capillary electrophoresis (CE). In recent years, they have been used for modification of sorbents or as sorbents in solid phase extr...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mahshid Majd Mina Yazdanpanah Mohammad Reza Bayatloo Saeed Nojavan Source Type: research

Simple-to-use and portable device for free chlorine determination based on microwave-assisted synthesized carbon dots and smartphone images
Talanta. 2021 Jul 1;229:122298. doi: 10.1016/j.talanta.2021.122298. Epub 2021 Mar 12.ABSTRACTA new portable and simple 3D printed device was designed for free chlorine determination in water samples. The analytical method was based on the quenching caused by free chlorine on the fluorescence emission of the carbon dots (CD) synthesized from citric acid and urea. The fluorescence was captured through the camera of a smartphone, which was coupled to the 3D printed device, and the images were processed using the RGB system by the ImageJ 1.51q software. The proposed method was selective and precise (RSD% 4.6, for n = 6), and t...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: D Uriarte E Vidal A Canals C E Domini M Garrido Source Type: research

Exploring bufferless iron speciation in seawater by Competitive Ligand Equilibration-Cathodic Stripping Voltammetry: Does pH control really matter?
Talanta. 2021 Jul 1;229:122300. doi: 10.1016/j.talanta.2021.122300. Epub 2021 Mar 11.ABSTRACTIron speciation in seawater is of the utmost importance as this element plays a central role in the regulation of primary productivity. Here we present the development of a CLE-CSV (Competitive Ligand Equilibration-Cathodic Stripping Voltammetry) procedure for iron speciation in seawater avoiding for the first time the use of the pH buffer (2,3-dihydroxynaphthalene is used as the added ligand, atmospheric oxygen as the catalytic enhancer and a 1 mL volume per sample aliquot). The unbuffered method was setup, validated by using know...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: F Sanvito D Monticelli Source Type: research

One-pot label-free dual-aptasensor as a chemiluminescent tool kit simultaneously detect adenosine triphosphate and chloramphenicol in foods
Talanta. 2021 Jul 1;229:122226. doi: 10.1016/j.talanta.2021.122226. Epub 2021 Mar 6.ABSTRACTThe chemiluminescence (CL) analysis based on label-free dual-aptasensor was developed for simultaneous detection of adenosine triphosphate (ATP) and chloramphenicol (CAP) in food. Magnetic microspheres and polystyrene microspheres used as separating and immobilizing carriers which immobilized the two different captured DNA, respectively. Then these carriers were put in the mixture of ATPs, CAPs, ATP-binding aptamers and CAP-binding aptamers to make one-pot label-free recognized interaction. The more ATP or CAP molecules binding thei...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Xiluan Yan Yuanlin Ji Yipi Xiao Xinxin Xue Jie Liu Su Li Fanrong Ai Xiangjuan Zheng Source Type: research

Simultaneous determination of short-chain fatty alcohols in aged oil and biodiesels by stable isotope labeling assisted liquid chromatography-mass spectrometry
Talanta. 2021 Jul 1;229:122223. doi: 10.1016/j.talanta.2021.122223. Epub 2021 Mar 9.ABSTRACTShort-chain fatty alcohols (SCFAs) are one of the reactants for the production of biodiesels. The SCFA residues at the trace level have a significant impact on biodiesel quality. However, the analysis of SCFAs in aged biodiesels has not been reported so far, which is probably due to the unavailability of an appropriate analytical method for the simultaneous determination of SCFAs. Herein, we developed a novel analytical approach with high sensitivity and selectivity for the simultaneous identification and determination of SCFAs in s...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Hua-Ming Xiao Shuai Zhao Ru-Ting Fan Dilshad Hussain Xian Wang Source Type: research

Chemometric pre-processing can negatively affect the performance of near-infrared spectroscopy models for fruit quality prediction
The objectives of this study are to test two hypotheses to explore the effect of pre-processing on NIR spectra of fresh fruit. The first hypothesis is that the pre-processing of NIR spectra with scatter correction techniques can reduce the predictive performance of models as the scatter correction can reduce the useful scattering information correlated to the property of interest. The second hypothesis is that the Deep Learning (DL) can model the raw absorbance data (mix of scattering and absorption) much more efficiently than the Partial Least Squares (PLS) regression analysis. To test the hypotheses, a real NIR data set ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Puneet Mishra Douglas N Rutledge Jean-Michel Roger Khan Wali Haris Ahmad Khan Source Type: research

A review on analytical methods with special reference to electroanalytical methods for the determination of some anticancer drugs in pharmaceutical and biological samples
Talanta. 2021 Jul 1;229:122247. doi: 10.1016/j.talanta.2021.122247. Epub 2021 Mar 7.ABSTRACTIt is widely accepted that cancer, the second leading cause of death, is a morbidity with big impacts on the global health. In the last few years, chemo-therapeutic treatment continually induces alone most lengthy consequents, which is extremely harmful for the physiological and psychological health of the patients. In the present research, we discuss the recent techniques for employed for extraction, and quantitative determination of such compounds in pharmaceutical, and biological specimens. In the frame of this information, this ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mohadeseh Safaei Masoud Reza Shishehbore Source Type: research

Off-flavor in soy drink: Development, optimization, and validation of an easy and fast method to quantify the key odorants
Talanta. 2021 Jul 1;229:122251. doi: 10.1016/j.talanta.2021.122251. Epub 2021 Mar 13.ABSTRACTA detailed molecular flavor profile was necessary to understand the low acceptance of soy drink by Western consumers. Accordingly, key odor-active compounds were detected by means of aroma dilution analyses coupled with gas chromatography-mass spectrometry-olfactometry after application of suitable solvent-free volatile extraction techniques. Four quantification methods (standard addition, external calibration, internal standard, and stable isotope dilution assay) were developed and validated to measure the concentrations after dir...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ann-Kathrin Nedele Nicole Mayer Natalie Feller J örg Hinrichs Yanyan Zhang Source Type: research

A simple, sensitive and efficient electrochemical platform based on carbon paste electrode modified with Fe < sub > 3 < /sub > O < sub > 4 < /sub > @MIP and graphene oxide for folic acid determination in different matrices
Talanta. 2021 Jul 1;229:122258. doi: 10.1016/j.talanta.2021.122258. Epub 2021 Mar 15.ABSTRACTA new biomimetic sensor selective to folic acid based on a carbon paste modified with graphene oxide and Fe3O4 nanoparticles coated with molecularly imprinted polymer in the core@shell format (Fe3O4@MIPs) was obtained using the polyol method. The sensing phase was synthesized in a simple way and characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Brunauer-Emmett-Teller (BET) surface area. In the binding experiments the selective material showed a high adsorption capacity (Q) of 30...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Sara Manente Garcia Ademar Wong Sabir Khan Maria D P T Sotomayor Source Type: research

Characterization of stem cell-like property in cancer cells based on single-cell impedance measurement in a microfluidic platform
Talanta. 2021 Jul 1;229:122259. doi: 10.1016/j.talanta.2021.122259. Epub 2021 Mar 9.ABSTRACTInvestigation of stem cell-like property in cancer cells is important for the development of new therapeutic drugs targeting at malignant tumors. Currently, the standard approach for identifying cancer stem cell-like cells relies on the recognition of stem cell surface markers. However, the reliability remains controversial among biologists. In the current work, a dielectrophoretic and impedimetric hybrid microfluidic platform was developed for capturing single cells and characterizing their stem cell-like property. Single cells wer...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Kin Fong Lei Yu-Chen Ho Chia-Hao Huang Chun-Hao Huang Ping Ching Pai Source Type: research

Voltammetry of 7-dehydrocholesterol as a new and useful tool for Smith-Lemli-Opitz syndrome diagnosis
This study shows for the first time that electrochemical oxidation of 7-dehydrocholesterol can be used for its voltammetric determination. Two classes of supporting electrolytes in acetonitrile and a mixture of acetonitrile-water were used: inorganic acids known to promote structural changes of steroids and indifferent electrolytes. Oxidation of 7-dehydrocholesterol at ca +0.8 V (vs. Ag/AgNO3 in acetonitrile) in 0.1 mol L-1 NaClO4 in acetonitrile is useful for its voltammetric detection using common bare electrode materials. Detection limits for 7-dehydrocholesterol lie in the low micromolar range for all the working elect...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Jan Klouda Lenka Bene šová Pavel Ko čovský Karolina Schwarzov á-Pecková Source Type: research

An improved strategy for analysis of lipid molecules utilising a reversed phase C < sub > 30 < /sub > UHPLC column and scheduled MS/MS acquisition
Talanta. 2021 Jul 1;229:122262. doi: 10.1016/j.talanta.2021.122262. Epub 2021 Mar 17.ABSTRACTMeasuring physiochemically diverse molecules (including lipids) which vary significantly in their concentrations poses a great analytical challenge. In untargeted lipidomics studies, reversed phase chromatography coupled with data-dependent MS/MS acquisition (DDA) is frequently applied. The optimal assay should deliver a high number of detected compounds with associated fragmentation data. In this work, we introduce novel 30 and 50 min UHPLC assays utilising lipid separation on a C30 stationary phase with a modified DDA strategy us...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Andris Jankevics Amelia Jenkins Warwick B Dunn Luk áš Najdekr Source Type: research

A novel fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH < sub > 2 < /sub > for low background and high sensitivity determination of H < sub > 2 < /sub > S
Talanta. 2021 Jul 1;229:122271. doi: 10.1016/j.talanta.2021.122271. Epub 2021 Mar 6.ABSTRACTHydrogen sulfide is typical metabolic marker and environmental pollutant which is worthwhile to determine. Herein, a low background and high sensitivity fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH2 is proposed for the determination of hydrogen sulfide. Firstly, a functional monomer 3,5-diaminobenzoic acid is employed to modify on the CAU-10-NH2, the product CAU-10-NH-dAba has strong fluorescent performance at 412 nm under an excitation wavelength of 320 nm. Subsequently, it is fur...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Wenpeng Gong Li Zhang Ying Yu Bixia Lin Yumin Wang Manli Guo Yujuan Cao Source Type: research

Sensitive detection of transcription factor by coupled fluorescence-encoded microsphere with exonuclease protection
Talanta. 2021 Jul 1;229:122272. doi: 10.1016/j.talanta.2021.122272. Epub 2021 Mar 3.ABSTRACTAberrant transcription factors (TFs) activities are closely related to the occurrence and development of various diseases. Herein, we presented a fluorescence-encoded microsphere-based approach for TFs detection coupling with common DNA footprinting assay. Target TFs specifically bound the binding sites of double-stranded DNA (dsDNA) probes which were conjugated to microspheres. Thus, the probes were protected from being hydrolyzed by exonuclease III (Exo III). Afterwards, biotins labeled on the probes reacted with streptavidin-phyc...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Yue Sun Liu Zang Choiwan Lau Xueji Zhang Jianzhong Lu Source Type: research

Application of magnetic nanomaterials in magnetic-chromatography: A review
Talanta. 2021 Jul 1;229:122273. doi: 10.1016/j.talanta.2021.122273. Epub 2021 Mar 9.ABSTRACTWith the advent of nanotechnology and its development, there have been dramatic advances in various aspects of diverse sciences. Nanotechnology encompasses the manipulating matter to create nanometre-scale materials with prodigious features and their implementation in a vast range of applications. The topic that is the current debate in today's scientific community and the transformation origin in modern technologies. Magnetic nanomaterials belong to the group of materials mainly consisting of a magnetic component, such as iron, and...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mahshid Manouchehri Shahram Seidi Fuad O Abdullah Source Type: research

Capture-SELEX for aptamer selection: A short review
Talanta. 2021 Jul 1;229:122274. doi: 10.1016/j.talanta.2021.122274. Epub 2021 Mar 5.ABSTRACTThe emerging aptamer, developed through the systematic evolution of ligands by exponential enrichment (SELEX) process, has revolutionized and facilitated the discoveries in basic research. Among all SELEX technology, Capture-SELEX is a variant of the in vitro selection process, which is suitable for isolating aptamers against small molecules. Capture-SELEX library was developed to enable the immobilization of the oligonucleotides instead of the target molecules during the aptamer selection process. The review provides an update on t...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Chen Lyu Imran Mahmood Khan Zhouping Wang Source Type: research

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites
Talanta. 2021 Jul 1;229:122275. doi: 10.1016/j.talanta.2021.122275. Epub 2021 Mar 15.ABSTRACTThere is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the d...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ayman Chmayssem Nicolas Verplanck Constantin Edi Tanase Guillaume Costa Karen Monsalve-Grijalba Simon Amigues M élanie Alias Maxime Gougis V éronique Mourier S éverine Vignoud Amir M Ghaemmaghami Pascal Mailley Source Type: research

A functional DNA-modified dual-response gold nanoprobe for simultaneously imaging the acidic microenvironment and membrane proteins of tumor cells
Talanta. 2021 Jul 1;229:122284. doi: 10.1016/j.talanta.2021.122284. Epub 2021 Mar 16.ABSTRACTTumor progression is a complicated process influenced by multiple factors, in which the acidic tumor microenvironment (TME) and altered tumor-associated membrane proteins (TA-MPs) are closely involved. Monitoring the status of these factors is of significance for tumor progression research. Here, we develop a novel probe for simultaneously imaging the acidic TME and TA-MPs in situ. In this probe, i-motif-forming sequences (strand I) are conjugated to a gold nanoparticle (AuNP) via gold-sulfur bonds for acid-response. Extended aptam...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ailing Kan Shuai Wang Liyan Zhang Xiaowen Xu Nan Zhang Wei Jiang Source Type: research

Application of magnetic nanomaterials in bioanalysis
Talanta. 2021 Jul 1;229:122285. doi: 10.1016/j.talanta.2021.122285. Epub 2021 Mar 12.ABSTRACTThe importance of magnetic nanomaterials and magnetic hybrid materials, which are classified as new generation materials, in analytical applications is increasingly understood, and research on the adaptation of these materials to analytical methods has gained momentum. Development of sample preparation techniques and sensor systems using magnetic nanomaterials for the analysis of inorganic, organic and biomolecules in biological samples, which are among the samples that analytical chemists work on most, are among the priority issue...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Erkan Yilmaz Gokhan Sarp Furkan Uzcan Ozgur Ozalp Mustafa Soylak Source Type: research

Toward a deeper and simpler understanding of serum protein-mediated transformations of magnetic nanoparticles by ICP-MS
Talanta. 2021 Jul 1;229:122287. doi: 10.1016/j.talanta.2021.122287. Epub 2021 Mar 11.ABSTRACTA great variety of magnetic nanomaterials are entering preclinical investigations with the objective to select the most promising candidates for diagnostic and therapeutic applications. For an analytical approach to be used as a high-throughput screening tool, simple and cost-efficient sample preparation protocol is a basiс prerequisite. Here, we demonstrate how the application of continuous magnetic field allows for quantitatively separating iron oxide magnetic nanoparticles from a mixture with human serum to facilitate monitorin...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Olga V Kuznetsova Maciej Jarosz Bernhard K Keppler Andrei R Timerbaev Source Type: research

Development of < sup > 148 < /sup > Gd analysis method using stable Gd
Talanta. 2021 Jul 1;229:122295. doi: 10.1016/j.talanta.2021.122295. Epub 2021 Mar 11.ABSTRACTThe analytical method of Gd determination was developed with the aim to analyse 148Gd in environmental and bioassay samples. It involves the use of anion exchange resin, extraction chromatography, and cation exchange resin. Alkaline fusion and calcium oxalate co-precipitation are used for solid samples dissolution and liquid samples preconcentration, respectively. Total method recovery was tested with natural Gd (157Gd) using ICP-QQQ-MS. A maximum total recovery of 75 % was obtained.PMID:33838781 | DOI:10.1016/j.talanta.2021.122295 (Source: Talanta)
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Grzegorz Olszewski Patric Lindahl Peter Frisk Mats Eriksson H åkan B L Pettersson Source Type: research

Recent advances and applications of cyclodextrins in magnetic solid phase extraction
Talanta. 2021 Jul 1;229:122296. doi: 10.1016/j.talanta.2021.122296. Epub 2021 Mar 17.ABSTRACTCyclodextrins (CDs) as a family of cyclic oligosaccharides are toroidal with a hydrophobic interior and a hydrophilic exterior. They are well-known for their ability to form host-guest inclusion complexes with different compounds. They are used as chiral stationary phases in high performance liquid chromatography (HPLC) and gas chromatography (GC) or as chiral reagents in the background electrolyte of capillary electrophoresis (CE). In recent years, they have been used for modification of sorbents or as sorbents in solid phase extr...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mahshid Majd Mina Yazdanpanah Mohammad Reza Bayatloo Saeed Nojavan Source Type: research

Simple-to-use and portable device for free chlorine determination based on microwave-assisted synthesized carbon dots and smartphone images
Talanta. 2021 Jul 1;229:122298. doi: 10.1016/j.talanta.2021.122298. Epub 2021 Mar 12.ABSTRACTA new portable and simple 3D printed device was designed for free chlorine determination in water samples. The analytical method was based on the quenching caused by free chlorine on the fluorescence emission of the carbon dots (CD) synthesized from citric acid and urea. The fluorescence was captured through the camera of a smartphone, which was coupled to the 3D printed device, and the images were processed using the RGB system by the ImageJ 1.51q software. The proposed method was selective and precise (RSD% 4.6, for n = 6), and t...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: D Uriarte E Vidal A Canals C E Domini M Garrido Source Type: research

Exploring bufferless iron speciation in seawater by Competitive Ligand Equilibration-Cathodic Stripping Voltammetry: Does pH control really matter?
Talanta. 2021 Jul 1;229:122300. doi: 10.1016/j.talanta.2021.122300. Epub 2021 Mar 11.ABSTRACTIron speciation in seawater is of the utmost importance as this element plays a central role in the regulation of primary productivity. Here we present the development of a CLE-CSV (Competitive Ligand Equilibration-Cathodic Stripping Voltammetry) procedure for iron speciation in seawater avoiding for the first time the use of the pH buffer (2,3-dihydroxynaphthalene is used as the added ligand, atmospheric oxygen as the catalytic enhancer and a 1 mL volume per sample aliquot). The unbuffered method was setup, validated by using know...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: F Sanvito D Monticelli Source Type: research

One-pot label-free dual-aptasensor as a chemiluminescent tool kit simultaneously detect adenosine triphosphate and chloramphenicol in foods
Talanta. 2021 Jul 1;229:122226. doi: 10.1016/j.talanta.2021.122226. Epub 2021 Mar 6.ABSTRACTThe chemiluminescence (CL) analysis based on label-free dual-aptasensor was developed for simultaneous detection of adenosine triphosphate (ATP) and chloramphenicol (CAP) in food. Magnetic microspheres and polystyrene microspheres used as separating and immobilizing carriers which immobilized the two different captured DNA, respectively. Then these carriers were put in the mixture of ATPs, CAPs, ATP-binding aptamers and CAP-binding aptamers to make one-pot label-free recognized interaction. The more ATP or CAP molecules binding thei...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Xiluan Yan Yuanlin Ji Yipi Xiao Xinxin Xue Jie Liu Su Li Fanrong Ai Xiangjuan Zheng Source Type: research

Simultaneous determination of short-chain fatty alcohols in aged oil and biodiesels by stable isotope labeling assisted liquid chromatography-mass spectrometry
Talanta. 2021 Jul 1;229:122223. doi: 10.1016/j.talanta.2021.122223. Epub 2021 Mar 9.ABSTRACTShort-chain fatty alcohols (SCFAs) are one of the reactants for the production of biodiesels. The SCFA residues at the trace level have a significant impact on biodiesel quality. However, the analysis of SCFAs in aged biodiesels has not been reported so far, which is probably due to the unavailability of an appropriate analytical method for the simultaneous determination of SCFAs. Herein, we developed a novel analytical approach with high sensitivity and selectivity for the simultaneous identification and determination of SCFAs in s...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Hua-Ming Xiao Shuai Zhao Ru-Ting Fan Dilshad Hussain Xian Wang Source Type: research

Chemometric pre-processing can negatively affect the performance of near-infrared spectroscopy models for fruit quality prediction
The objectives of this study are to test two hypotheses to explore the effect of pre-processing on NIR spectra of fresh fruit. The first hypothesis is that the pre-processing of NIR spectra with scatter correction techniques can reduce the predictive performance of models as the scatter correction can reduce the useful scattering information correlated to the property of interest. The second hypothesis is that the Deep Learning (DL) can model the raw absorbance data (mix of scattering and absorption) much more efficiently than the Partial Least Squares (PLS) regression analysis. To test the hypotheses, a real NIR data set ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Puneet Mishra Douglas N Rutledge Jean-Michel Roger Khan Wali Haris Ahmad Khan Source Type: research

A review on analytical methods with special reference to electroanalytical methods for the determination of some anticancer drugs in pharmaceutical and biological samples
Talanta. 2021 Jul 1;229:122247. doi: 10.1016/j.talanta.2021.122247. Epub 2021 Mar 7.ABSTRACTIt is widely accepted that cancer, the second leading cause of death, is a morbidity with big impacts on the global health. In the last few years, chemo-therapeutic treatment continually induces alone most lengthy consequents, which is extremely harmful for the physiological and psychological health of the patients. In the present research, we discuss the recent techniques for employed for extraction, and quantitative determination of such compounds in pharmaceutical, and biological specimens. In the frame of this information, this ...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Mohadeseh Safaei Masoud Reza Shishehbore Source Type: research

Off-flavor in soy drink: Development, optimization, and validation of an easy and fast method to quantify the key odorants
Talanta. 2021 Jul 1;229:122251. doi: 10.1016/j.talanta.2021.122251. Epub 2021 Mar 13.ABSTRACTA detailed molecular flavor profile was necessary to understand the low acceptance of soy drink by Western consumers. Accordingly, key odor-active compounds were detected by means of aroma dilution analyses coupled with gas chromatography-mass spectrometry-olfactometry after application of suitable solvent-free volatile extraction techniques. Four quantification methods (standard addition, external calibration, internal standard, and stable isotope dilution assay) were developed and validated to measure the concentrations after dir...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Ann-Kathrin Nedele Nicole Mayer Natalie Feller J örg Hinrichs Yanyan Zhang Source Type: research

A simple, sensitive and efficient electrochemical platform based on carbon paste electrode modified with Fe < sub > 3 < /sub > O < sub > 4 < /sub > @MIP and graphene oxide for folic acid determination in different matrices
Talanta. 2021 Jul 1;229:122258. doi: 10.1016/j.talanta.2021.122258. Epub 2021 Mar 15.ABSTRACTA new biomimetic sensor selective to folic acid based on a carbon paste modified with graphene oxide and Fe3O4 nanoparticles coated with molecularly imprinted polymer in the core@shell format (Fe3O4@MIPs) was obtained using the polyol method. The sensing phase was synthesized in a simple way and characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Brunauer-Emmett-Teller (BET) surface area. In the binding experiments the selective material showed a high adsorption capacity (Q) of 30...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Sara Manente Garcia Ademar Wong Sabir Khan Maria D P T Sotomayor Source Type: research

Characterization of stem cell-like property in cancer cells based on single-cell impedance measurement in a microfluidic platform
Talanta. 2021 Jul 1;229:122259. doi: 10.1016/j.talanta.2021.122259. Epub 2021 Mar 9.ABSTRACTInvestigation of stem cell-like property in cancer cells is important for the development of new therapeutic drugs targeting at malignant tumors. Currently, the standard approach for identifying cancer stem cell-like cells relies on the recognition of stem cell surface markers. However, the reliability remains controversial among biologists. In the current work, a dielectrophoretic and impedimetric hybrid microfluidic platform was developed for capturing single cells and characterizing their stem cell-like property. Single cells wer...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Kin Fong Lei Yu-Chen Ho Chia-Hao Huang Chun-Hao Huang Ping Ching Pai Source Type: research

Voltammetry of 7-dehydrocholesterol as a new and useful tool for Smith-Lemli-Opitz syndrome diagnosis
This study shows for the first time that electrochemical oxidation of 7-dehydrocholesterol can be used for its voltammetric determination. Two classes of supporting electrolytes in acetonitrile and a mixture of acetonitrile-water were used: inorganic acids known to promote structural changes of steroids and indifferent electrolytes. Oxidation of 7-dehydrocholesterol at ca +0.8 V (vs. Ag/AgNO3 in acetonitrile) in 0.1 mol L-1 NaClO4 in acetonitrile is useful for its voltammetric detection using common bare electrode materials. Detection limits for 7-dehydrocholesterol lie in the low micromolar range for all the working elect...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Jan Klouda Lenka Bene šová Pavel Ko čovský Karolina Schwarzov á-Pecková Source Type: research

An improved strategy for analysis of lipid molecules utilising a reversed phase C < sub > 30 < /sub > UHPLC column and scheduled MS/MS acquisition
Talanta. 2021 Jul 1;229:122262. doi: 10.1016/j.talanta.2021.122262. Epub 2021 Mar 17.ABSTRACTMeasuring physiochemically diverse molecules (including lipids) which vary significantly in their concentrations poses a great analytical challenge. In untargeted lipidomics studies, reversed phase chromatography coupled with data-dependent MS/MS acquisition (DDA) is frequently applied. The optimal assay should deliver a high number of detected compounds with associated fragmentation data. In this work, we introduce novel 30 and 50 min UHPLC assays utilising lipid separation on a C30 stationary phase with a modified DDA strategy us...
Source: Talanta - April 11, 2021 Category: Chemistry Authors: Andris Jankevics Amelia Jenkins Warwick B Dunn Luk áš Najdekr Source Type: research