A new reagent for in vivo structure probing of RNA G and U residues that improves RNA structure prediction alone and combined with DMS
RNA. 2024 Apr 26:rna.079974.124. doi: 10.1261/rna.079974.124. Online ahead of print.ABSTRACTA key to understanding the roles of RNA in regulating gene expression is knowing their structures in vivo. One way to obtain this information is through probing structures of RNA with chemicals. To probe RNA structure directly in cells, membrane-permeable reagents that modify the Watson-Crick (WC) face of unpaired nucleotides can be used. While dimethyl sulfate (DMS) has led to substantial insight into RNA structure, it has limited nucleotide specificity in vivo, with WC face reactivity only at adenine (A) and cytosine (C) at neutra...
Source: RNA - April 26, 2024 Category: Genetics & Stem Cells Authors: Catherine A Douds Paul Babitzke Philip Bevilacqua Source Type: research
Antimicrobial Resistance as Risk Factor for Recurrent Bacteremia after Staphylococcus aureus, Escherichia coli, or Klebsiella spp. Community-Onset Bacteremia
Emerg Infect Dis. 2024 May;30(5):974-983. doi: 10.3201/eid3005.231555.ABSTRACTWe investigated links between antimicrobial resistance in community-onset bacteremia and 1-year bacteremia recurrence by using the clinical data warehouse of Europe's largest university hospital group in France. We included adult patients hospitalized with an incident community-onset Staphylococcus aureus, Escherichia coli, or Klebsiella spp. bacteremia during 2017-2019. We assessed risk factors of 1-year recurrence using Fine-Gray regression models. Of the 3,617 patients included, 291 (8.0%) had >1 recurrence episode. Third-generation cephalo...
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Salam Abbara Didier Guillemot David R M Smith Salma El Oualydy Maeva Kos C écile Poret St éphane Breant Christian Brun-Buisson Laurence Watier Source Type: research
Economic Burden of Acute Gastroenteritis among Members of Integrated Healthcare Delivery System, United States, 2014-2016
Emerg Infect Dis. 2024 May;30(5):968-973. doi: 10.3201/eid3005.230356.ABSTRACTWe conducted a large surveillance study among members of an integrated healthcare delivery system in Pacific Northwest of the United States to estimate medical costs attributable to medically attended acute gastroenteritis (MAAGE) on the day care was sought and during 30-day follow-up. We used multivariable regression to compare costs of MAAGE and non-MAAGE cases matched on age, gender, and index time. Differences accounted for confounders, including race, ethnicity, and history of chronic underlying conditions. Analyses included 73,140 MAAGE epi...
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: John F Dickerson Suzanne B Salas Judy Donald Holly C Groom Mi H Lee Claire P Mattison Aron J Hall Mark A Schmidt Source Type: research
Antigenic Characterization of Novel Human Norovirus GII.4 Variants San Francisco 2017 and Hong Kong 2019
Emerg Infect Dis. 2024 May;30(5):1026-1029. doi: 10.3201/eid3005.231694.ABSTRACTNorovirus is a major cause of acute gastroenteritis; GII.4 is the predominant strain in humans. Recently, 2 new GII.4 variants, Hong Kong 2019 and San Francisco 2017, were reported. Characterization using GII.4 monoclonal antibodies and serum demonstrated different antigenic profiles for the new variants compared with historical variants.PMID:38666659 | DOI:10.3201/eid3005.231694 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Kentaro Tohma Michael Landivar Lauren A Ford-Siltz Kelsey A Pilewski Joseph A Kendra Sandra Niendorf Gabriel I Parra Source Type: research
Detection of OXA-181 Carbapenemase in Shigella flexneri
We report the detection of OXA-181 carbapenemase in an azithromycin-resistant Shigella spp. bacteria in an immunocompromised patient. The emergence of OXA-181 in Shigella spp. bacteria raises concerns about the global dissemination of carbapenem resistance in Enterobacterales and its implications for the treatment of infections caused by Shigella bacteria.PMID:38666725 | DOI:10.3201/eid3005.231558 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Ghulam Dhabaan Hassan Jamal Danielle Ouellette Sarah Alexander Karen Arane Aaron Campigotto Manal Tadros Pierre-Philippe Pich é-Renaud Source Type: research
An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis
Conclusions: A high correlation of the QIAstat-Dx ME panel with reference methods was shown. QIAstat-Dx ME is a rapid-PCR technique to be applied in patients with suspected ME with a high accuracy.PMID:38667448 | PMC:PMC11048994 | DOI:10.3390/diagnostics14080802 (Source: Herpes)
Source: Herpes - April 26, 2024 Category: Infectious Diseases Authors: Genoveva Cuesta Pedro Puerta-Alcalde Andrea Vergara Enric Roses Jordi Bosch Climent Casals-Pascual Alex Soriano M ª Ángeles Marcos Sergi Sanz Jordi Vila Source Type: research
Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys
Vaccine. 2024 Apr 25:S0264-410X(24)00483-3. doi: 10.1016/j.vaccine.2024.04.056. Online ahead of print.ABSTRACTGroup A rotavirus (RVA) is the primary etiological agent of acute gastroenteritis (AGE) in children under 5 years of age. Despite the global implementation of vaccines, rotavirus infections continue to cause over 120,000 deaths annually, with a majority occurring in developing nations. Among infants, the P[8] rotavirus strain is the most prevalent and can be categorized into four distinct lineages. In this investigation, we expressed five VP4(aa26-476) proteins from different P[8] lineages of human rotavirus in E. ...
Source: Vaccine - April 26, 2024 Category: Allergy & Immunology Authors: Guoxing Luo Yuanjun Zeng Roufang Sheng Zhishan Zhang Cao Li Han Yang Yaling Chen Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia Source Type: research
Prevalence and Resistance Patterns of Urinary Tract Infection in Al-Madinah Al-Munawarah, Saudi Arabia: A Retrospective Study
CONCLUSION: This study reported UT mono-infection and poly-infection in Al-Madinah Al-Munawarah, Saudi Arabia, with a predominant representation from gram-negative bacteria, Enterobacteriaceae. Most of the UT microbial strains showed a highly resistant profile.PMID:38665033 | DOI:10.24976/Discov.Med.202436183.80 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Areej A Alhhazmi Rawan A Alhazmi Eilaf S Alahmadi Wafa M Almuallim Zainab I Aljurfi Mohammad A Alturkostani Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Mol Biol Cell - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Lipidomic approach to identify Escherichia coli and Shigella spp. by matrix-assisted laser desorption/ionization mass spectrometry
CONCLUSIONS: Identification of bacteria from lipid fingerprints obtained by the MALDI MS technique is possible and may become a useful tool in the future, especially for microorganisms that are difficult to distinguish by other methods.PMID:38670227 | DOI:10.1016/j.advms.2024.04.005 (Source: Advances in Medical Sciences)
Source: Advances in Medical Sciences - April 26, 2024 Category: Biomedical Science Authors: Adrian Arendowski Source Type: research
A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel for infected wounds
Int J Biol Macromol. 2024 Apr 24:131795. doi: 10.1016/j.ijbiomac.2024.131795. Online ahead of print.ABSTRACTBacterial infections during wound healing impede the healing process and trigger local or systemic inflammatory reactions. Consequently, there is an urgent need to develop a new material with antimicrobial and antioxidant properties to promote infected wound healing. A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel (HMn) is prepared by employing MnO2 nanosheets into 4ARM-PEG5000-SH crosslinked methacrylated hyaluronic acid (HAMA) network. The coordination between sulfhydryl groups of 4ARM-PEG5...
Source: International Journal of Biological Macromolecules - April 26, 2024 Category: Biochemistry Authors: Yongping Lu Weiqi Kang Yue Yu Haiying Lu Yuemin Wang Zhe Xu Jia Zeng Meng Qin Xinyuan Xu Source Type: research
Human serum albumin directed formation of cadmium telluride quantum dots: Applications in biosensing, anti-bacterial activities and cell cytotoxicity measurements
Int J Biol Macromol. 2024 Apr 24:131862. doi: 10.1016/j.ijbiomac.2024.131862. Online ahead of print.ABSTRACTAlthough cadmium-based quantum dots (QDs) are highly promising candidates for numerous biological applications, their intrinsic toxicity limits their pertinency in living systems. Surface functionalization of QDs with appropriate molecules could reduce the toxicity level. Herein, we have synthesized the smaller sized (1-5 nm) aqueous-compatible biogenic CdTe QDs using human serum albumin (HSA) as a surface passivating agent via a greener approach. HSA-functionalized CdTe QDs have been explored in multiple in vitro se...
Source: International Journal of Biological Macromolecules - April 26, 2024 Category: Biochemistry Authors: Mahabul Haque Mitul Kalita Dipak Chamlagai Sona Lyndem Sudipta Koley Puja Kumari Kripamoy Aguan Atanu Singha Roy Source Type: research
Identification of a homoarginine biosynthetic gene from a microcystin biosynthetic pathway
This study provides new insights into the logic underpinning the biosynthesis of microcystin chemical variants and broadens our knowledge of structural diversity of microcystins.PMID:38670499 | DOI:10.1016/j.toxicon.2024.107733 (Source: Toxicon)
Source: Toxicon - April 26, 2024 Category: Toxicology Authors: Xiaodan Ouyang Matti Wahlsten Maija Pollari Endrews Delbaje Jouni Jokela David P Fewer Source Type: research
Antigenic Characterization of Novel Human Norovirus GII.4 Variants San Francisco 2017 and Hong Kong 2019
Emerg Infect Dis. 2024 May;30(5):1026-1029. doi: 10.3201/eid3005.231694.ABSTRACTNorovirus is a major cause of acute gastroenteritis; GII.4 is the predominant strain in humans. Recently, 2 new GII.4 variants, Hong Kong 2019 and San Francisco 2017, were reported. Characterization using GII.4 monoclonal antibodies and serum demonstrated different antigenic profiles for the new variants compared with historical variants.PMID:38666659 | DOI:10.3201/eid3005.231694 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Kentaro Tohma Michael Landivar Lauren A Ford-Siltz Kelsey A Pilewski Joseph A Kendra Sandra Niendorf Gabriel I Parra Source Type: research
