Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917
This study utilized AlphaFold2, SERS (Surface-enhanced Raman spectroscopy), and CD (circular dichroism) techniques to analyze the structural characteristics of FliCEcN and its mutants, FliCΔ174-506 and FliCΔ274-406, and tested their immune effects by stimulating Caco-2 cells in vitro. The results indicate that the D2 and D3 domains of FliCEcN have more complex interactions compared to the D1-D2 domain pair., and these domains also play a role in molecular docking with TLR5 (Toll-like receptor 5). Furthermore, FliCΔ274-406 has more missing side chain and characteristic amino acid peaks than FliCΔ174-506. The FliCEcN gro...
Source: Archives of Microbiology - April 18, 2024 Category: Microbiology Authors: Shuang Li Ming Wen Guilan Wen Ying Yang Source Type: research
Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research
The influence of pH on the efficacy of oxidation-reduction potential (ORP) to predict chlorine disinfection of surrogate bacteria, Escherichia coli O157:H7 and Listeria monocytogenes in oxidant demand free conditions and fresh produce wash water
Food Microbiol. 2024 Aug;121:104516. doi: 10.1016/j.fm.2024.104516. Epub 2024 Mar 14.ABSTRACTOxidation-reduction potential (ORP) is commonly used as a rapid measurement of the antimicrobial potential of free chlorine during industrial fresh produce washing. The current study tested the hypothesis that ORP can act as a "single variable" measurement of bacterial (vegetative and endospores) inactivation effectiveness with free chlorine irrespective of the water pH value. This situation has on occasion been assumed but never confirmed nor disproven. Chlorine-dosed pH 6.5 and 8.5 phosphate buffer solutions were inoculated with ...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Kimberly Gongora Joris Vankerschaver Imca Sampers Sam Van Haute Source Type: research
A meta-analysis of microbial thermal inactivation in low moisture foods
In this study, we leveraged the body of previous work on this topic to model key experimental features that determine microbial thermal inactivation in low moisture foods. We identified 27 studies which contained 782 mean D-values and developed linear mixed-effect models to assess the effect of microorganism type, matrix structure and composition, water activity, temperature, and inoculation and recovery methods on cell death kinetics. Intraclass correlation statistics (I2) and conditional R2 values of the linear mixed effects models were: E. coli (R2-0.91, I2-83%), fungi (R2-0.88, I2-85%), L. monocytogenes (R2-0.84, I2-75...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Yadwinder Singh Rana Long Chen Yang Jiao Lynn M Johnson Abigail B Snyder Source Type: research
Characterization of diarrheagenic Escherichia coli from different cattle production systems in Brazil
This study aimed to characterize the distribution of DEC [Shiga toxin-producing E. coli (STEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC)] from extensive and intensive cattle production systems in Brazil. Samples (n = 919) were collected from animal feces (n = 200), carcasses (n = 600), meat cuts (n = 90), employee feces (n = 9), and slaughterhouse water (n = 20). Virulence genes were detected by PCR in 10% of animal samples (94/919), with STEC (n = 81) as the higher prevalence, followed by EIEC (n = 8), and lastly EPEC (n = 5). Anim...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Rafaela de Melo Tavares Mallu Jagnow Sereno Aryele Nunes da Cruz Encide Sampaio Juliano Gon çalves Pereira Luciano Dos Santos Bersot Ricardo Seiti Yamatogi Douglas Ruben Call Lu ís Augusto Nero Source Type: research
Essential oil of < em > Eugenia stictopetala < /em > Mart. ex DC. has antioxidant, antibacterial, and gastrointestinal protective effect
Nat Prod Res. 2024 Apr 18:1-5. doi: 10.1080/14786419.2024.2342556. Online ahead of print.ABSTRACTThis work aimed to investigate the chemical composition, antioxidant capacity, antibacterial activity, and intestinal and gastric protective effects, in addition to the acute toxicity of the essential oil of E. stictopetala (EOEs). (E)-caryophyllene (18.01%), β-pinene (8.84%), and (E)-nerolidol (8.24%), were the components found in higher content in the essential oil. The EOEs showed antioxidant capacity with IC50 values ranging from 220.28 to 283.67 μg/mL, in addition to presenting antibacterial activity against Escherichia ...
Source: Natural Product Research - April 18, 2024 Category: Biochemistry Authors: Bruno Oliveira de Veras Rianne Ferreira Felix Geovanna Maria de Medeiros Moura Daniela Maria do Amaral Ferraz Navarro J úlio César Ribeiro de Oliveira Farias de Aguiar Almir Gon çalves Wanderley M árcia Vanusa da Silva Maria Tereza Dos Santos Correia Source Type: research
Significance of colonization by antibiotic-resistant organisms prior to congenital heart disease surgery in children from low- to middle-income countries sent by non-governmental organizations to Switzerland
ConclusionPrevalence of AMROs carriage is high in children from LMIC and has a limited impact on patients ’ outcome. However, ARMOs carriage leads to higher consumption of antibiotics. Screening may help saving use of broad-spectrum antibiotic in non-carrier patients. (Source: Infection)
Source: Infection - April 18, 2024 Category: Infectious Diseases Source Type: research
Epidemiology and antimicrobial susceptibility profiles of Enterobacterales causing bloodstream infections before and during COVID-19 pandemic: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART) in Taiwan, 2018-2021
CONCLUSIONS: The COVID-19 pandemic appears to be associated with a general decrease in antimicrobial susceptibility rates among Enterobacterales causing BSIs in Taiwan. Continuous surveillance is crucial to monitor antimicrobial resistance during the pandemic and in the future.PMID:38632023 | DOI:10.1016/j.jmii.2024.04.004 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - April 17, 2024 Category: Microbiology Authors: Yu-Lin Lee Chun-Eng Liu Hung-Jen Tang Yu-Tsung Huang Yao-Shen Chen Po-Ren Hsueh SMART Taiwan Group Source Type: research
Epidemiology and antimicrobial susceptibility profiles of Enterobacterales causing bloodstream infections before and during COVID-19 pandemic: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART) in Taiwan, 2018-2021
CONCLUSIONS: The COVID-19 pandemic appears to be associated with a general decrease in antimicrobial susceptibility rates among Enterobacterales causing BSIs in Taiwan. Continuous surveillance is crucial to monitor antimicrobial resistance during the pandemic and in the future.PMID:38632023 | DOI:10.1016/j.jmii.2024.04.004 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - April 17, 2024 Category: Microbiology Authors: Yu-Lin Lee Chun-Eng Liu Hung-Jen Tang Yu-Tsung Huang Yao-Shen Chen Po-Ren Hsueh SMART Taiwan Group Source Type: research
Epidemiology and antimicrobial susceptibility profiles of Enterobacterales causing bloodstream infections before and during COVID-19 pandemic: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART) in Taiwan, 2018-2021
CONCLUSIONS: The COVID-19 pandemic appears to be associated with a general decrease in antimicrobial susceptibility rates among Enterobacterales causing BSIs in Taiwan. Continuous surveillance is crucial to monitor antimicrobial resistance during the pandemic and in the future.PMID:38632023 | DOI:10.1016/j.jmii.2024.04.004 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - April 17, 2024 Category: Microbiology Authors: Yu-Lin Lee Chun-Eng Liu Hung-Jen Tang Yu-Tsung Huang Yao-Shen Chen Po-Ren Hsueh SMART Taiwan Group Source Type: research
Correlation between the development of phage resistance and the original antibiotic resistance of host bacteria under the co-exposure of antibiotic and bacteriophage
This study explores phage resistance development in hosts with different antibiotic resistance and helps to understand the limitations and possible solutions of phage-based technologies.PMID:38631474 | DOI:10.1016/j.envres.2024.118921 (Source: Environmental Research)
Source: Environmental Research - April 17, 2024 Category: Environmental Health Authors: Lingli Li Mengya Zhou Ming Yu Xu Ren Linzhi Li Chunjun Shen Chunping Deng Yucheng Liu Bing Yang Source Type: research
Lipase and Protease Production Ability of Multi-drug Resistant Bacteria Worsens the Outcomes of Wound Infections
CONCLUSION: In conclusion, the findings suggest that lipases and proteases produced by bacteria could contribute to drug resistance and act as virulence factors in the development of surgical site infections. Understanding the role of these enzymes may inform strategies for preventing and managing post-surgical wound infections more effectively.PMID:38629357 | DOI:10.2174/0113816128302189240402043330 (Source: Current Pharmaceutical Design)
Source: Current Pharmaceutical Design - April 17, 2024 Category: Drugs & Pharmacology Authors: Attaur Rahman Saiqa Sardar Zeeshan Niaz Asif Khan None Sheheryar Abdulwahed Fahad Alrefaei Muhammad Hamayun Sajid Ali Source Type: research
Influence of the use of remediated soil and agricultural drainage water on the safety of tomato fruits
In conclusion, the employed bioremediation techniques successfully reduced the PTE content and microbial load in both soil and drainage water ecosystems and in harvested tomato fruits. Henceforth, no health risks are expected from the consumption of this product.PMID:38632200 | DOI:10.1007/s11356-024-33187-z (Source: Environmental Science and Pollution Research International)
Source: Environmental Science and Pollution Research International - April 17, 2024 Category: Environmental Health Authors: Salah H Salem Mohamed Saber Samir Gadow Hoda Kabary Alaa Zaghloul Source Type: research
Epidemiology and antimicrobial susceptibility profiles of Enterobacterales causing bloodstream infections before and during COVID-19 pandemic: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART) in Taiwan, 2018-2021
CONCLUSIONS: The COVID-19 pandemic appears to be associated with a general decrease in antimicrobial susceptibility rates among Enterobacterales causing BSIs in Taiwan. Continuous surveillance is crucial to monitor antimicrobial resistance during the pandemic and in the future.PMID:38632023 | DOI:10.1016/j.jmii.2024.04.004 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - April 17, 2024 Category: Microbiology Authors: Yu-Lin Lee Chun-Eng Liu Hung-Jen Tang Yu-Tsung Huang Yao-Shen Chen Po-Ren Hsueh SMART Taiwan Group Source Type: research
