The modification landscape of P. aeruginosa tRNAs
RNA. 2024 Apr 29:rna.080004.124. doi: 10.1261/rna.080004.124. Online ahead of print.ABSTRACTRNA modifications have a substantial impact on tRNA function, with modifications in the anticodon loop contributing to translational fidelity and modifications in the tRNA core impacting structural stability. In bacteria, tRNA modifications are crucial for responding to stress and regulating the expression of virulence factors. Although tRNA modifications are well-characterized in a few model organisms, our knowledge of tRNA modifications in human pathogens, such as Pseudomonas aeruginosa, remains limited. Here we leveraged two orth...
Source: RNA - April 29, 2024 Category: Genetics & Stem Cells Authors: Mariana D Mandler Siddhardha S Maligireddy Wilfried M Guiblet Christina M Fitzsimmons Kayla S McDonald Delayna L Warrell Pedro J Batista Source Type: research
Synthesis and bioevaluation of water-soluble < em > β < /em > -cyclodextrin-diterpene acid conjugates
This study suggested that the multivalent diterpene acid derivatives may have potential application against coronaviruses as entry inhibitors.PMID:38684026 | DOI:10.1080/14786419.2024.2347449 (Source: Natural Product Research)
Source: Natural Product Research - April 29, 2024 Category: Biochemistry Authors: Hua LiWen Yi Yanliang Elena V Tretyakova Anna A Smirnova Oxana B Kazakova Sulong Xiao Source Type: research
NMR fingerprinting of depolymerised durian rind pectin and its antibacterial activity
This study investigated the effect of commercial sterilisation treatment (121 °C for 15 min) on durian rind pectin. The change of structure was observed by NMR (nuclear magnetic resonance) and the antibacterial activity was assessed by microdilution method to obtain the minimum inhibitory concentration (MIC) value. NMR spectra revealed the rhamnogalacturonan-I and homogalacturonan structure, with lower methyl-ester in sterilised pectin. Native pectin was unable to inhibit Escherichia coli and Staphylococcus aureus at the highest tested concentration (MIC > 25 mg/mL), but sterilised pectin showed inhibitory effect again...
Source: Natural Product Research - April 29, 2024 Category: Biochemistry Authors: Inas Suci Rahmawati Nancy Dewi Yuliana Azis Boing Sitanggang Harsi Dewantari Kusumaningrum Source Type: research
The emergence of carbapenem-resistance and New Delhi metallo- β-lactamase-1 ( < em > bla < /em > < sub > NDM-1 < /sub > ) among < em > Salmonella < /em > spp. in Kerman, Iran
CONCLUSION: Our study showed the emergence of carbapenem-resistant and blaNDM-1 among Salmonella spp. for the first time in Kerman, Iran. Since Salmonella spp. plays an important role in the transmission of resistance genes in livestock and humans in the food chains, so more stringent control policies are recommended to prevent the circulation of drug-resistant and potentially pathogenic strains from animals to humans.PMID:38682060 | PMC:PMC11055441 | DOI:10.18502/ijm.v16i1.14868 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 29, 2024 Category: Microbiology Authors: Sanaz Dehdashti Parvin Mohseni Reza Ghanbarpour Sajad Aslani Maryam-Sadat Moradiyan Davood Kalantar-Neyestanaki Source Type: research
The antibiogram of pus cultures in federal tertiary care hospital, Islamabad and its utility in antimicrobial stewardship
CONCLUSION: The generation of a local antibiogram specific to the hospital setting is essential to effectively manage infections empirically and preserve the efficacy of existing antibiotics. By implementing antimicrobial stewardship practices based on a better understanding of antibiotic susceptibility patterns, we can contribute to the mitigation of antibiotic resistance and improve patient outcomes.PMID:38682051 | PMC:PMC11055436 | DOI:10.18502/ijm.v16i1.14871 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - April 29, 2024 Category: Microbiology Authors: Farhan Kursheed Asraar Tabassum Umme Farwa Samia Wazir Muhammad Shafiq Ahmareen Khalid Sheikh Source Type: research
Prevalence of AmpC beta-lactamase and extended spectrum beta-lactamase co-producer in Escherichia coli and Klebsiella species in a teaching hospital
CONCLUSION: Distinguishing between AmpC β-lactamase and ESBL-producing organisms has epidemiological significance as well as therapeutic importance. Moreover, AmpC β-lactamase and ESBLs co-producing organisms can lead to false negative ESBL confirmatory test. Therefore, knowing the local prevalence can guide the clinician in navigating the treatment.PMID:38682847 (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 29, 2024 Category: Pathology Authors: S M V Ting Z Ismail A Hanafiah Source Type: research
Environmentally friendly silver nanoparticles synthesized from Verbascum nudatum var. extract and evaluation of its versatile biological properties and dye degradation activity
Environ Sci Pollut Res Int. 2024 Apr 29. doi: 10.1007/s11356-024-33424-5. Online ahead of print.ABSTRACTIn the present study, green synthesis of silver nanoparticles (VNE-AgNPs) via Verbascum nudatum extract was carried out for the first time. The synthesized AgNPs were characterized by different spectral methods such as UV-vis, FTIR, XRD, TEM, and EDAX. According to TEM analyses, the average size range of AgNPs was 17-21 nm, and the dominant peaks in the 111°, 200°, 221°, and 311° planes in the XRD pattern indicated the Ag-NPs FCC crystal structure. FTIR data showed that VNE-AgNPs interacted with many reducing, cappin...
Source: Cancer Control - April 29, 2024 Category: Cancer & Oncology Authors: Ömer Hazman Gofur Khamidov Mustafa Abdullah Yilmaz Mehmet Fatih Bozkurt Mustafa Kargio ğlu Davlat Tukhtaev Ibrahim Erol Source Type: research
Enteropathogenic Viruses associated with Acute Gastroenteritis among African Children under 5 years of age: A Systematic Review and Meta-analysis
We present data from thirty-three (33) eligible studies published between 2003 and 2023 from African countries bearing the brunt of the virus-associated diarrheal mortality. Random effects meta-analysis with proportion, subgroups, and meta-regression analyses were employed. Overall, rotavirus with estimated pooled prevalence of 31.0% (95% CI 24.0 –39.0) predominated in all primary care visits and hospitalizations, followed by norovirus, adenovirus, sapovirus, astrovirus, and aichivirus with pooled prevalence estimated at 15.0% (95% CI 12.0–20.0), 10% (95% CI 6-15), 4.0% (95% CI 2.0–6.0), 4% (95% CI 3-6), and 2.3% (95...
Source: Journal of Infection - April 29, 2024 Category: Infectious Diseases Authors: Cornelius Arome Omatola, Ropo Ebenezer Ogunsakin, Anyebe Bernard Onoja, Martin-Luther Oseni Okolo, Joseph Abraham-Oyiguh, Kehinde Charles Mofolorunso, Phoebe Queen Akoh, Omebije Patience Adejo, Joshua Idakwo, Therisa Ojomideju Okeme, Danjuma Muhammed, Dav Source Type: research
Efflux pump inhibitor chlorpromazine effectively increases the susceptibility of Escherichia coli to antimicrobial peptide Brevinin-2CE
Future Microbiology, Ahead of Print. (Source: Future Microbiology)
Source: Future Microbiology - April 29, 2024 Category: Microbiology Authors: Cao Bing An Mengjuan Ma Xinyu Zhu Chixin Tan Xinyao Sun Yan Li Zhi Source Type: research
< i > KmerAperture < /i > : Retaining < i > k < /i > -mer synteny for alignment-free extraction of core and accessory differences between bacterial genomes
by Matthew P. Moore, Mirjam Laager, Paolo Ribeca, Xavier Didelot
By decomposing genome sequences intok-mers, it is possible to estimate genome differences without alignment. Techniques such ask-mer minimisers, for example MinHash, have been developed and are often accurate approximations of distances based on fullk-mer sets. These and other alignment-free methods avoid the large temporal and computational expense of alignment. However, thesek-mer set comparisons are not entirely accurate within-species and can be completely inaccurate within-lineage. This is due, in part, to their inability to distinguish core polymorphis...
Source: PLoS Genetics - April 29, 2024 Category: Genetics & Stem Cells Authors: Matthew P. Moore Source Type: research
Immunization of turkeys with < i > Clostridium septicum < /i > alpha toxin-based recombinant subunit proteins can confer protection against experimental Clostridial dermatitis
by Feba Ann John, Valeria Criollo, Carissa Gaghan, Abigail Armwood, Jennifer Holmes, Anil J. Thachil, Rocio Crespo, Raveendra R. Kulkarni
Clostridial dermatitis (CD), caused byClostridium septicum, is an emerging disease of increasing economic importance in turkeys. Currently, there are no effective vaccines for CD control. Here, two non-toxic domains ofC.septicum alpha toxin, namely ntATX-D1 and ntATX-D2, were identified, cloned, and expressed inEscherichia coli as recombinant subunit proteins to investigate their use as potential vaccine candidates. Experimental groups consisted of a Negative control (NCx) that did not ...
Source: PLoS One - April 29, 2024 Category: Biomedical Science Authors: Feba Ann John Source Type: research
Insights into the antibacterial effectiveness of linalool against Shigella flexneri on pork surface: Changes in bacterial growth and pork quality
This study aimed to examine the effectiveness of linalool (a natural preservative) against S. flexneri and explore its potential application in contaminated pork. The results showed that linalool was capable of damaging the cell membrane and binding to the DNA of S. flexneri, and inhibiting biofilm formation and disrupting mature biofilms. The antibacterial effectiveness of linalool on the surface of pork was further demonstrated by analyzing the physicochemical properties of the pork (i.e., weight loss rate, pH value, color index, and TVB-N value) and its protein profiles. Linalool did not completely kill S. flexneri in p...
Source: International Journal of Food Microbiology - April 28, 2024 Category: Food Science Authors: Xueying Song Jiamu Kang Xingyan Wei Liu Liu Yongfeng Liu Feng Wang Source Type: research
Human health risk assessment framework for new water resource recovery-based bio-composite materials
J Water Health. 2024 Apr;22(4):652-672. doi: 10.2166/wh.2024.168. Epub 2024 Feb 22.ABSTRACTA new type of bio-composite material is being produced from water-recovered resources such as cellulose fibres from wastewater, calcite from the drinking water softening process, and grass and reed from waterboard sites. These raw materials may be contaminated with pathogens and chemicals such as Escherichia coli, heavy metals, and resin compounds. A novel risk assessment framework is proposed here, addressing human health risks during the production of new bio-composite materials. The developed framework consists of a combination of...
Source: Journal of Water and Health - April 28, 2024 Category: Environmental Health Authors: Arianna Nativio Oriana Jovanovic Zoran Kapelan Jan Peter van der Hoek Source Type: research
The heme binding protein ChuX is a regulator of heme degradation by the ChuS protein in Escherichia coli O157:H7
J Inorg Biochem. 2024 Apr 24;256:112575. doi: 10.1016/j.jinorgbio.2024.112575. Online ahead of print.ABSTRACTEscherichia coli O157:H7 possesses an 8-gene cluster (chu genes) that contains genes involved in heme transport and processing from the human host. Among the chu genes, four encode cytoplasmic proteins (ChuS, ChuX, ChuY and ChuW). ChuX was previously shown to be a heme binding protein and to assist ChuW in heme degradation under anaerobic conditions. The purpose of this work was to investigate if ChuX works in concert with ChuS, which is a protein able to degrade heme by a non-canonical mechanism and release the iro...
Source: Journal of Inorganic Biochemistry - April 28, 2024 Category: Biochemistry Authors: Danrong Ye Phuong Trang Nguyen Steve Bourgault Manon Couture Source Type: research
Structure-based drug design of DNA minor groove binders and evaluation of their antibacterial and anticancer properties
This study shed light on the mechanism of action of MGBs and revealed the important structural features for their antitumor and antibacterial activities, which are important to guide future development of MGB derivatives as novel antibacterial and anticancer agents.PMID:38678825 | DOI:10.1016/j.ejmech.2024.116440 (Source: European Journal of Medicinal Chemistry)
Source: European Journal of Medicinal Chemistry - April 28, 2024 Category: Chemistry Authors: Hasan Y Alniss Hadeel M Al-Jubeh Yousef A Msallam Ruqaiyyah Siddiqui Zinb Makhlouf Anil Ravi Rania Hamdy Sameh S M Soliman Naveed A Khan Source Type: research
