Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea
CONCLUSIONS: Childhood diarrhea can lead to increasingly severe nutrient deficiencies. Steatorrhea is the hallmark of malabsorption, and a stool test, such as the acid steatocrit, can be routinely used as a laboratory tool for the semi-quantitative evaluation of fat malabsorption in diarrheic children.PMID:38648349 | DOI:10.7705/biomedica.6913 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - April 22, 2024 Category: International Medicine & Public Health Authors: Adson Santos Martins Samara Alves Santos Cl áudia Alves da Silva Lisboa T ânia Fraga Barros Tereza Cristina Medrado Ribeiro Hugo Da Costa-Ribeiro Ângela Peixoto de Mattos Patr ícia Silva de Almeida Mendes Carlos Maur ício Cardeal Mendes Edna L úcia Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea
CONCLUSIONS: Childhood diarrhea can lead to increasingly severe nutrient deficiencies. Steatorrhea is the hallmark of malabsorption, and a stool test, such as the acid steatocrit, can be routinely used as a laboratory tool for the semi-quantitative evaluation of fat malabsorption in diarrheic children.PMID:38648349 | DOI:10.7705/biomedica.6913 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - April 22, 2024 Category: International Medicine & Public Health Authors: Adson Santos Martins Samara Alves Santos Cl áudia Alves da Silva Lisboa T ânia Fraga Barros Tereza Cristina Medrado Ribeiro Hugo Da Costa-Ribeiro Ângela Peixoto de Mattos Patr ícia Silva de Almeida Mendes Carlos Maur ício Cardeal Mendes Edna L úcia Source Type: research
Melittin-The principal toxin of honeybee venom-Is also produced in the honeybee fat body
Comp Biochem Physiol C Toxicol Pharmacol. 2024 Apr 20;281:109928. doi: 10.1016/j.cbpc.2024.109928. Online ahead of print.ABSTRACTMelittin is a powerful toxin present in honeybee venom that is active in a wide range of animals, from insects to humans. Melittin exerts numerous biological, toxicological, and pharmacological effects, the most important of which is destruction of the cell membrane. The phospholipase activity of melittin and its ability to activate phospholipases in the venom contribute to these actions. Using analytical methods, we discovered that the honeybee Apis mellifera produces melittin not only in the ve...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - April 22, 2024 Category: Biochemistry Authors: Mark éta Hejníková Ale š Tomčala Jan Černý Dalibor Kodr ík Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Expression and Purification of His-Tagged Variants of Human Hepatitis A Virus 3C Protease
CONCLUSION: We demonstrated the applicability of our approach for producing recombinant, enzymatically active 3Cpro.PMID:38644721 | DOI:10.2174/0109298665293548240327082821 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - April 22, 2024 Category: Biochemistry Authors: Maria A Karaseva Vladislav A Gramma Dina R Safina Natalia A Lunina Alexey A Komissarov Sergey V Kostrov Ilya V Demidyuk Source Type: research
Drying-wetting cycle enhances stress resistance of Escherichia coli O157:H7 in a model soil
This study provides new insights into bacteria behavior under changing soil moisture and this may explain O157:H7 outbreaks following rainfall and flooding, as the dry-wet cycle promotes stress cross-resistance development.PMID:38648967 | DOI:10.1016/j.envpol.2024.123988 (Source: Environmental Pollution)
Source: Environmental Pollution - April 22, 2024 Category: Environmental Health Authors: Jing Se Yinan Xie Qingxu Ma Lin Zhu Yulong Fu Xin Xu Chaofeng Shen Paolo Nannipieri Source Type: research
Melittin-The principal toxin of honeybee venom-Is also produced in the honeybee fat body
Comp Biochem Physiol C Toxicol Pharmacol. 2024 Apr 20:109928. doi: 10.1016/j.cbpc.2024.109928. Online ahead of print.ABSTRACTMelittin is a powerful toxin present in honeybee venom that is active in a wide range of animals, from insects to humans. Melittin exerts numerous biological, toxicological, and pharmacological effects, the most important of which is destruction of the cell membrane. The phospholipase activity of melittin and its ability to activate phospholipases in the venom contribute to these actions. Using analytical methods, we discovered that the honeybee Apis mellifera produces melittin not only in the venom ...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - April 22, 2024 Category: Biochemistry Authors: Mark éta Hejníková Ale š Tomčala Jan Černý Dalibor Kodr ík Source Type: research
Oligomerization states of the Mycobacterium tuberculosis RNA polymerase core and holoenzymes
Arch Microbiol. 2024 Apr 22;206(5):230. doi: 10.1007/s00203-024-03955-z.ABSTRACTDuring the past few decades, a wealth of knowledge has been made available for the transcription machinery in bacteria from the structural, functional and mechanistic point of view. However, comparatively little is known about the homooligomerization of the multisubunit M. tuberculosis RNA polymerase (RNAP) enzyme and its functional relevance. While E. coli RNAP has been extensively studied, many aspects of RNAP of the deadly pathogenic M. tuberculosis are still unclear. We used biophysical and biochemical methods to study the oligomerization s...
Source: Archives of Microbiology - April 22, 2024 Category: Microbiology Authors: Sandrea Maureen Francis Shehna Pattar Kadavan Ramanathan Natesh Source Type: research
Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea
CONCLUSIONS: Childhood diarrhea can lead to increasingly severe nutrient deficiencies. Steatorrhea is the hallmark of malabsorption, and a stool test, such as the acid steatocrit, can be routinely used as a laboratory tool for the semi-quantitative evaluation of fat malabsorption in diarrheic children.PMID:38648349 | DOI:10.7705/biomedica.6913 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - April 22, 2024 Category: International Medicine & Public Health Authors: Adson Santos Martins Samara Alves Santos Cl áudia Alves da Silva Lisboa T ânia Fraga Barros Tereza Cristina Medrado Ribeiro Hugo Da Costa-Ribeiro Ângela Peixoto de Mattos Patr ícia Silva de Almeida Mendes Carlos Maur ício Cardeal Mendes Edna L úcia Source Type: research
Drying-wetting cycle enhances stress resistance of Escherichia coli O157:H7 in a model soil
This study provides new insights into bacteria behavior under changing soil moisture and this may explain O157:H7 outbreaks following rainfall and flooding, as the dry-wet cycle promotes stress cross-resistance development.PMID:38648967 | DOI:10.1016/j.envpol.2024.123988 (Source: Environmental Pollution)
Source: Environmental Pollution - April 22, 2024 Category: Environmental Health Authors: Jing Se Yinan Xie Qingxu Ma Lin Zhu Yulong Fu Xin Xu Chaofeng Shen Paolo Nannipieri Source Type: research
Expression and Purification of His-Tagged Variants of Human Hepatitis A Virus 3C Protease
CONCLUSION: We demonstrated the applicability of our approach for producing recombinant, enzymatically active 3Cpro.PMID:38644721 | DOI:10.2174/0109298665293548240327082821 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - April 22, 2024 Category: Biochemistry Authors: Maria A Karaseva Vladislav A Gramma Dina R Safina Natalia A Lunina Alexey A Komissarov Sergey V Kostrov Ilya V Demidyuk Source Type: research
