Effects of chitosan-loaded hydroxyapatite on osteoblasts and osteosarcoma for chemopreventative applications.
In this study, chitosan was directly loaded onto HA disks to evaluate its in vitro release and effects on human fetal osteoblast (hFOB) and human osteosarcoma (MG-63) cell lines. It is hypothesized that the sustained release of chitosan will decrease osteosarcoma cell proliferation and enhance proliferation of osteoblast cells. Through morphological characterization and MTT assay analysis, chitosan showed no toxicity to human fetal osteoblast (hFOB) cells. Chitosan was also shown to decrease human osteosarcoma cell viability by up to 96% compared to control samples. This suggests a pro-apoptotic mechanism against osteosarc...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Koski C, Vu AA, Bose S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fabrication of a sensitive label free electrochemical immunosensor for detection of prostate specific antigen using functionalized multi-walled carbon nanotubes/polyaniline/AuNPs.
Abstract The aim of this research is to introduce a novel label free electrochemical immunosensor based on glassy carbon electrode (GCE) modified with carboxylated carbon nanotubes (COOH-MWCNTs)/polyaniline (PANI)/gold nanoparticles (AuNPs) for the detection of prostate specific antigen (PSA). The AuNPs were utilized as a connector for PSA antibody immobilization through NH2 groups on antibody. Investigations on modified electrode surface were performed by FT-IR spectrum, scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) to evaluate the synthesized nanocomposite and modified electro...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Assari P, Rafati AA, Feizollahi A, Joghani RA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A new way to prepare gold nanoparticles by sputtering - Sterilization, stability and other properties.
te;ková L, Kolská Z, Švorčík V Abstract We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine. The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG). The PEG was used as a basic biocompatible stabilizer of the AuNP colloid. In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stab...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Pišlová M, Kolářová K, Vokatá B, Brož A, Ulbrich P, Bačáková L, Kolská Z, Švorčík V Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Valorization of Pichia spent medium via one-pot synthesis of biocompatible silver nanoparticles with potent antioxidant, antimicrobial, tyrosinase inhibitory and reusable catalytic activities.
Abstract The purpose of this study was to determine the feasibility of a novel biogenic synthesis of silver nanoparticles using Pichia pastoris spent medium - a major waste product from heterologous protein expression as the sole reducing and capping agent, with potential biological, antimicrobial and wastewater remediation applications. Using UV-vis spectroscopy, TEM, XRD, EDX, FTIR, SEM we demonstrated the successful bio-fabrication of Pichia spent medium encapsulated silver nanoparticles (PSM-AgNPs). PSM-AgNPs displayed substantial antiradical activity against DPPH and ABTS. The antiradical activity against ABT...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Eze FN, Nwabor OF Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Attenuation of cadmium-induced vascular toxicity by pro-angiogenic nanorods.
Abstract Cadmium (Cd) is a common heavy metal that causes major environmental pollution with adverse effects on human health and well-being. Exposure to Cd is known to exhibit detrimental consequences on all the vital organ systems of the body, especially the vascular system. Certain approaches using anti-oxidants and chelating agents have been demonstrated previously to mitigate Cd-induced toxicity. However, these approaches are associated with their own limitations. In this context, there is a critical need for the development of alternative treatment strategies to address the conditions associated with Cd-poiso...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Roy A, Nethi SK, Suganya N, Raval M, Chatterjee S, Patra CR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Prolonged-release of menthol through a superhydrophilic multilayered structure of balangu (Lallemantia royleana)-gelatin nanofibers.
This study aimed to develop a sandwich structure based on electrospun mats derived from gelatin (central layer) and Balangu seed gum (outer layers) and to compare its capability for prolonging the menthol release in the oral phase compared to the gelatin monolayer mat. The mesh-like structure and the smooth and uniform surface of the electrospun mats designed in this study were authenticated by Atomic Force Microscopy (AFM). By designing the sandwich structure, the dissolution time and contact angle of the mats were increased and their bioadhesive strength decreased. The swelling degree of the gelatin mat (453.25 &plu...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Rezaeinia H, Ghorani B, Emadzadeh B, Mohebbi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hemocompatibility of gallium-68 labeled iron oxide nanoparticles coated with 2,3-dicarboxypropane-1,1-diphosphonic acid.
los D Abstract Dual-modality contrast agents (DMCA), such as radiolabeled magnetic nanoparticles, have attracted significant attention in diagnostic applications due to their potency for the timely and accurate diagnosis of diseases. The hemocompatibility of a candidate DMCA with human blood is essential for the investigation of its application in vivo. In this respect, here we focused on the evaluation of the hemocompatibility of a new DMCA, that is based on iron oxide nanoparticles (i.e. Fe3O4 magnetite), with human red blood cells (RBCs). The specific iron oxide nanoparticles are surface functionalized with 2,3...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Karageorgou MA, Bouziotis P, Vranješ-Djurić S, Stamopoulos D Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair. Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase. The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a d...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Lin X, Guan X, Wu Y, Zhuang S, Wu Y, Du L, Zhao J, Rong J, Zhao J, Tu M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

The effects of severe plastic deformation on the mechanical and corrosion characteristics of a bioresorbable Mg-ZKQX6000 alloy.
Abstract In this work, a bioresorbable Mg-ZKQX6000 (Mg-6Zn-0.6Zr-0.4Ag-0.2Ca (wt%)) alloy was severely plastically deformed via equal channel angular pressing (ECAP) according to three unique hybrid routes at low temperatures (200 °C to 125 °C). The roles of ECAP processing on microstructure, and ensuing mechanical properties and corrosion rates, are assessed. Microstructurally, ECAP induces a complex plethora of features, especially variations in grain sizes and precipitates' sizes, distributions, and morphologies for individual cases. Mechanically, ECAP generally refined grain size, resulting i...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Vaughan MW, Karayan AI, Srivastava A, Mansoor B, Seitz JM, Eifler R, Karaman I, Castaneda H, Maier HJ Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Rapid regeneration of enamel-like-oriented inorganic crystals by using rotary evaporation.
Abstract Enamel, the hardest tissue in the human body, has excellent mechanical properties, mainly due to its highly ordered spatial structure. Fabricating enamel-like structure is still a challenge today. In this work, a simple and highly efficient method was introduced, using the silk fibroin as a template to regulate calcium- and phosphate- supersaturated solution to regenerate enamel-like hydroxyapatite crystals on various substrates (enamel, dentin, titanium, and polyethylene) under rotary evaporation. The enamel-like zinc oxide nanorod array structure was also successfully synthesized using the aforementione...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Wang S, Zhang L, Chen W, Jin H, Zhang Y, Wu L, Shao H, Fang Z, He X, Zheng S, Cao CY, Wong HM, Li Q Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Citrate zinc hydroxyapatite nanorods with enhanced cytocompatibility and osteogenesis for bone regeneration.
Abstract The development of biomaterials that mimicking the hydroxyapatite nanoparticles existent in the immature bone tissue is crucial, especially to accelerate the bone remodeling and regeneration. In this work, it was developed for the first time, hydroxyapatite nanoparticles (NPs) incorporating citrate and zinc (cit-Zn-Hap) in their composition towards a one-step hydrothermal procedure. For comparison purposes, hydroxyapatite NPs incorporating only zinc (Zn-Hap) or citrate (cit-Hap), as well as hydroxyapatite without any of these elements (Hap) were synthesised. The physicochemical characterization was carrie...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Fernandes MH, Alves MM, Cebotarenco M, Ribeiro IAC, Grenho L, Gomes PS, Carmezim MJ, Santos CF Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fabrication and evaluation of electrospun biomimetic sulphonated PEEK nanofibrous scaffold for human skin cell proliferation and wound regeneration potential.
In this study an attempt has been carried out to design and develop sulphonated polyether ether ketone (SPEEK) nanofibrous scaffold to explore its role on skin cell proliferation potential. 2 h-SPEEK portrayed the highest proliferative potential for HaCaT keratinocytes and fibroblasts. It was aimed for the tailored release of bio-actives from the spatiotemporally designed Aloe vera incorporated 2 h-SPEEK nanoscaffold to accelerate the skin wound regeneration. FTIR, EDX and XRD analyses revealed the effective incorporation of Aloe vera in the electrospun nanofibers. SEM analysis revealed the nano-topographical mor...
Source: Appl Human Sci - July 2, 2020 Category: Physiology Authors: Ekambaram R, Dharmalingam S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Silver molybdate nanoparticles based immunosensor for the non-invasive detection of Interleukin-8 biomarker.
In this study, we report the silver molybdate nanoparticles (β-Ag2MoO4 NPs) based non-invasive and sensitive electrochemical immunosensor for label-free detection of Interleukin-8 (IL-8) biomarker. The X-ray diffraction and Raman spectroscopy studies confirm the cubic spinel structures of β-Ag2MoO4 NPs. High-resolution transmission electron microscopy study depicted average size of β-Ag2MoO4 NPs as 27.15 nm. The cleaned indium tin oxide coated glass substrates were coated with spin-coated thin films of Ag2MoO4 NPs. These electrodes used for covalently immobilization of antibodies specific to IL-8 (Anti-...
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Pachauri N, Lakshmi GBVS, Sri S, Gupta PK, Solanki PR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Design of high-ductile medium entropy alloys for dental implants.
Abstract Developing new materials with high strength and ductility, low modulus and high biocompatibility is a continuing demand in the field of surgical implants. Inspired by the high-entropy design philosophy, two medium entropy alloys (MEAs), i.e. equiatomic TiZrHf and equi-weight Ti40Zr20Hf10Nb20Ta10 were designed and their mechanical properties and biocompatibility were assessed. Both the single-phase hexagonal close-packed (HCP) structured TiZrHf alloy and the single-phase body-centered cubic (BCC) structured Ti40Zr20Hf10Nb20Ta10 alloy show high strength-ductility combinations close to commercial Ti-6Al-4V w...
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Wang S, Wu D, She H, Wu M, Shu D, Dong A, Lai H, Sun B Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

An injectable, self-assembled multicellular microsphere with the incorporation of fibroblast-derived extracellular matrix for therapeutic angiogenesis.
In this study, we propose a self-assembled, injectable multicellular microspheres containing human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cell (MSCs), collagen hydrogel (Col), and hFDM toward therapeutic angiogenesis. Those multicellular microspheres are spontaneously formed by the mixtures of cell and hydrogel after being dropped on the parafilm for several hours. The size of microspheres can be manipulated via adjusting the initial volume of droplets and the culture period. The cells in the microspheres are highly viable. Multicellular microspheres show good capability of cell migration on 2D cult...
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Du P, Da Costa ADS, Savitri C, Ha SS, Wang PY, Park K Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Graphene oxide coated Titanium Surfaces with Osteoimmunomodulatory Role to Enhance Osteogenesis.
In this study, GO was coated on pure titanium using dopamine. GO-coated titanium (Ti-GO) surfaces exhibited good biocompatibility, with the ability to stimulate the expression of osteogenic genes, and extracellular matrix mineralization in human mesenchymal stromal cells (hMSCs). Interestingly, it was found that GO-coated surfaces could manipulate the polarization of macrophages and expression of inflammatory cytokines via the Toll-like receptor pathway. Under physiological conditions, Ti-GO activated macrophages and induced mild inflammation and a pro-osteogenic environment, characterized by a slight increase in the level...
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Su J, Du Z, Xiao L, Wei F, Yang Y, Li M, Qiu Y, Liu J, Chen J, Xiao Y Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Bioinspired 3D porous human placental derived extracellular matrix/silk fibroin sponges for accelerated bone regeneration.
In this study, porous hybrid placental extracellular matrix sponge (PIMS) was fabricated by a combinatorial method using silk fibroin (SF)/placental derived extracellular matrix and subsequently evaluated its efficacy towards bone tissue regeneration. The presence of intrinsic growth factors was evidenced by immunoblotting of the extracted proteins derived from the placental derived extracellular matrix. This growth factor rich PIMS lends a unique bioactive scaffolding to human amniotic mesenchymal stem cells (HAMSCs) which supported enhanced proliferation as well as superior osteogenic differentiation. Gene expression stu...
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Rameshbabu AP, Bankoti K, Datta S, Subramani E, Apoorva A, Ghosh P, Jana S, Manchikanti P, Roy S, Chaudhury K, Dhara S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Carbon dots doped tungstic anhydride on graphene oxide nanopanels: A new picomolar-range creatinine selective enzymeless electrochemical sensor.
In this study, a new non-enzymatic electrochemical probe, carbon dots doped tungstic anhydride embedded on graphene oxide nanopanels (CDs/WO3@GO) is reported for picomolar-level Crt detection in blood and urine with a wide linear range (0.2-112.0 nM). The sensor is economical, reproducible, stable and interferents-free. The properties of CDs/WO3@GO were studied using various analytical techniques. The proposed electrochemical Crt sensor could be used as a sustainable alternative for diagnostic use. PMID: 32487413 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - June 5, 2020 Category: Physiology Authors: Ponnaiah SK, Prakash P Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Degradable porous drug-loaded polymer scaffolds for localized cancer drug delivery and breast cell/tissue growth.
Abstract This paper presents the results of a combined experimental and analytical study of blended FDA-approved polymers [polylactic-co-glycolic acid (PLGA), polyethylene glycol (PEG) and polycaprolactone (PCL)] with the potential for sustained localized cancer drug release. Porous drug-loaded 3D degradable PLGA-PEG and PLGA-PCL scaffolds were fabricated using a multistage process that involved solvent casting and particulate leaching with lyophilization. The physicochemical properties including the mechanical, thermal and biostructural properties of the drug-loaded microporous scaffolds were characterized. The r...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Obayemi JD, Jusu SM, Salifu AA, Ghahremani S, Tadesse M, Uzonwanne VO, Soboyejo WO Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Controlling porous titanium/soft tissue interactions with an innovative surface chemical treatment: Responses of macrophages and fibroblasts.
In this study, we present a controlled oxidation-based surface treatment of microbead-based porous titanium implants which not only increases the colonization by connective tissue cells but also decreases the macrophage attachment. The treatment created a nanotextured surface on the implants with an acidic shift of isoelectric point (from 4.09 to 3.09) without endangering implant's mechanical integrity. The attachment and metabolic activity of activated macrophages were significantly lower on treated surfaces with an increase in the secretion of anti-inflammatory IL-1RA and a decrease in pro-fibrotic CCL-18. Human fibrobla...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Barthes J, Cazzola M, Muller C, Dollinger C, Debry C, Ferraris S, Spriano S, Vrana NE Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Curcumin and silver nanoparticles carried out from polysaccharide-based hydrogels improved the photodynamic properties of curcumin through metal-enhanced singlet oxygen effect.
Abstract Colorectal cancer (CRC) has a high incidence and resistance to conventional treatments. Curcumin (CUR) is a promising natural product in the treatment of CRC with excellent in vitro results. However, its low bioavailability is a limiting factor in clinical applications. To overcome, CUR was incorporated into hydrogels constituted by chitosan (CHT) and chondroitin sulfate (CS), natural biopolymers, capable of controlled release. Hydrogels were synthesized in ionic liquids (ILs, [Hmim][HSO4]) improving the solubility of CHT and the hydrogel properties. Furthermore, CUR was combined with silver nanoparticles...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: de Freitas CF, Kimura E, Rubira AF, Muniz EC Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Anodized anisotropic titanium surfaces for enhanced guidance of gingival fibroblasts.
This study provides a proof-of-concept towards using anodization for improving soft-tissue sealing around dental abutment surfaces, with implications towards reducing implant failure/peri-implantitis and achieving long-term success, especially in compromised patient conditions. PMID: 32409032 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Gulati K, Moon HJ, Kumar PTS, Han P, Ivanovski S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
Abstract Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings. First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent. The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface. Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting me...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Ambrogi V, Pietrella D, Donnadio A, Latterini L, Di Michele A, Luffarelli I, Ricci M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Simultaneous sensing of copper, lead, cadmium and mercury traces in human blood serum using orthorhombic phase aluminium ferrite.
We report a facile, one-step solid-state synthesis of aluminium ferrite (AFO) for simultaneous sensing of heavy metal ions (HMIs) namely cadmium (Cd2+), lead (Pb2+), copper (Cu2+) and mercury (Hg2+) in human blood serum. The nanoflake morphology and orthorhombic phase formation of the as-synthesised AFO were revealed through field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD) pattern, respectively. The AFO modified glassy carbon electrode (AFO/GCE) exhibits distinct peak potentials for all HMIs with peak separation potential (ΔΕP) of 190 mV, 494 mV and 304 mV for Cd2...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Durai L, Badhulika S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Site-specific delivery of a natural chemotherapeutic agent to human lung cancer cells using biotinylated 2D rGO nanocarriers.
Abstract Chemotherapy has remained one of the most commonly employed treatment modalities for cancer. Despite the clinical availability of a large number of chemotherapeutic agents, the uncontrolled systemic distribution and the associated harmful side effects of chemotherapeutic agents pose major challenges demanding concerted efforts to enhance their cancer targetability. The layered structure of two-dimensional (2D) materials offers new opportunities by increasing the drug pay-load influencing the drug-release kinetics in a cancer micro-environment and facilitating targetability through the large accessible sur...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Gupta N, Bhagat S, Singh M, Jangid AK, Bansal V, Singh S, Pooja D, Kulhari H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synthesis of bio-mediated silver nanoparticles from Silybum marianum and their biological and clinical activities.
Abstract The purpose of current study was green synthesis of silver nanoparticles (AgNPs) from seeds and wild Silybum plants in comparison with their respective extracts followed by characterization and biological potency. The biologically synthesized AgNPs were subjected to characterization using techniques like XRD, FTIR, TEM, HPLC and SPE. Highly crystalline and stable NPs were obtained using Silybum wild plant (NP1) and seeds (NP3) with size range between 18.12 and 13.20 nm respectively. The synthesized NPs and their respective extracts revealed a vast range of biological applications showing antibacteria...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Shah M, Nawaz S, Jan H, Uddin N, Ali A, Anjum S, Giglioli-Guivarc'h N, Hano C, Abbasi BH Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

BSA-capped gold nanoclusters as potential theragnostic for skin diseases: Photoactivation, skin penetration, in vitro, and in vivo toxicity.
Abstract BSA-capped gold nanoclusters are promising theragnostic systems that can be excited to render both fluorescence emission and reactive oxygen species. Although their synthesis and photoluminescence properties are already well described, more accurate information about their use as photosensitizers is required in order to advance towards health applications. In this work, we have obtained BSA-capped gold nanoclusters and characterized their photophysics by different techniques. Singlet oxygen production was detected upon irradiation, which was enough to produce toxicity on two cell lines. Remarkably, an int...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Lillo CR, Calienni MN, Rivas Aiello B, Prieto MJ, Rodriguez Sartori D, Tuninetti J, Toledo P, Alonso SDV, Moya S, Gonzalez MC, Montanari J, Soler-Illia GJAA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Nitrogen-doped MoS2 quantum dots: Facile synthesis and application for the assay of hematin in human blood.
Abstract Nitrogen-doped MoS2 quantum dots (N-MoS2 QDs) were synthesized via a facile hydrothermal approach, and exhibited high fluorescence quantum yield (QY, 14.9%), excellent photostability, biocompatibility and water solubility. A novel method with good selectivity and sensitivity was established to assay hematin using N-MoS2 QDs as a fluorescent probe based on inner filter effect (IFE). Fluorescent quenching of N-MoS2 QDs has a fine linear dependence with the concentration of hematin in the range of 0.5-15 μmol/L and a limit of detection of 0.32 μmol/L (S/N = 3). By the detection meth...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Wu FY, Cheng YS, Wang DM, Li ML, Lu WS, Xu XY, Zhou XH, Wei XW Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agents.
Abstract Antimicrobial resistance is a complex global health challenge today. Discovery and development of new natural alternates with novel targets is utmost priority. In this experiment, alternative antibiotic agents in the form of silver nanoparticles (SNPs) and Achillea millefolium L. extracts were evaluated for antibacterial and antioxidant activity. The SNPs were synthesized using aqueous, ethanol and methanol extracts of A. millefolium and were monitored by a color change and UV-vis spectroscopy. The size and shape of the nanoparticles were determined through scanning electron microscopy and phase was asses...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Yousaf H, Mehmood A, Ahmad KS, Raffi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

3D-bioprinted functional and biomimetic hydrogel scaffolds incorporated with nanosilicates to promote bone healing in rat calvarial defect model.
Abstract Three-dimensional (3D) bioprinting is an extremely convenient biofabrication technique for creating biomimetic tissue-engineered bone constructs and has promising applications in regenerative medicine. However, existing bioinks have shown low mechanical strength, poor osteoinductive ability, and lacking a suitable microenvironment for laden cells. Nanosilicate (nSi) has shown to be a promising biomaterial, due to its unique properties such as excellent biocompatibility, degrade into nontoxic products, and with osteoinductive properties, which has been used in bone bioprinting. However, the long term bone ...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Liu B, Li J, Lei X, Cheng P, Song Y, Gao Y, Hu J, Wang C, Zhang S, Li D, Wu H, Sang H, Bi L, Pei G Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Modification of chlorosulfonated polystyrene substrates for bioanalytical applications.
N, Pavlov V Abstract In this work the modification of polystyrene micro-well plates and their use as bioanalytical platform is described. A wet-chemical procedure was applied for the chlorosulfonation of these polystyrene substrates (PS) resulting in well-controlled and reactive surfaces. This method enabled the production of transparent and stable substrates under ambient conditions. The chlorosulfonyl moieties at the substrate surface were converted under mild conditions into different functional groups. The modification of PS served to increase the hydrophilic properties of the surface and thus, the improvement...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Díez-Buitrago B, Fernández-SanArgimiro FJ, Lorenzo J, Briz N, Pavlov V Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Gold nanoparticle-assisted enhancement in bioactive properties of Australian native plant extracts, Tasmannia lanceolata and Backhousia citriodora.
In this study, gold nanoparticles (Au-NPs) were synthesised by following a one-step green synthesis, a reaction between HAuCl4 and biological molecules present in Tasmannia lanceolata leaf extract as a sole agent for both reduction and stabilisation. The characterisation techniques confirmed the successful synthesis of Au-NPs. TEM photograph revealed spherical shape nanoparticles with an average size of 7.10 ± 0.66 nm. The in-vitro cytotoxicity of Au-NPs was performed by analysing the percentage inhibition of cell viability using Resazurin assay on human liver cancer (HepG2), melanoma cancer (MM418 C1...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Khandanlou R, Murthy V, Wang H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineering and evaluation of forcespun functionalized carbon nano-onions reinforced poly ( ε-caprolactone) composite nanofibers for pH-responsive drug release.
Engineering and evaluation of forcespun functionalized carbon nano-onions reinforced poly (ε-caprolactone) composite nanofibers for pH-responsive drug release. Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110928 Authors: Mamidi N, Zuníga AE, Villela-Castrejón J Abstract Nanofibers and smart polymers are potentially fascinating biomaterials for the sustained release of therapeutic agents and tissue engineering applications. The current study describes a new class of pH-controlled polycaprolactone/mercaptophenyl methacrylate functionalized carbon nano-onions (PCL/f-CNOs) composite...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Mamidi N, Zuníga AE, Villela-Castrejón J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Multifunctional alginate nanoparticles containing nitric oxide donor and silver nanoparticles for biomedical applications.
ra AB Abstract Nitric oxide (NO) is an endogenous molecule with antimicrobial activity. Silver nanoparticles (AgNPs) are also well known for its antimicrobial properties. In this work, we prepared, characterized, evaluated the cytotoxicity and the antibacterial activity of alginate nanoparticles (alginate NPs) containing S-nitroso-mercaptosuccinic acid (S-nitroso-MSA, a NO donor) and/or AgNPs. AgNPs were obtained by plant mediated synthesis using green tea (Camellia sinensis), according to the green principles. Alginate NPs were obtained by ionotropic gelation with calcium ions (Ca2+) and used to incorporate the N...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Urzedo AL, Gonçalves MC, Nascimento MHM, Lombello CB, Nakazato G, Seabra AB Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Green synthesis of nanometal impregnated biomass - antiviral potential.
In conclusion, green synthesized nano-biomaterials can act as good antiviral agents. These nano-biomaterials can further be processed for formulation of effective and promising nanomedicines against CHIKV and other similar viruses which are a threat to human health. PMID: 32409081 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Choudhary S, Kumar R, Dalal U, Tomar S, Reddy SN Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineering hollow mesoporous silica nanoparticles to increase cytotoxicity.
cute;nez C, García-Muñoz RA Abstract Hollow mesoporous silica nanoparticles (HMSNs) consist of a network of cavities confined by mesoporous shells that have emerged as promising tools for drug delivery or diagnostic. The physicochemical properties of HMSNs are dictated by the synthesis conditions but which conditions affect which property and how it impacts on biological interactions is unclear. Here by changing the concentration of the structure-directing agent (SDA), the pH and the ratio between SDA and added salt (NaCl) we determine the effects in size, morphology, surface charge and density or de...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Pérez-Garnes M, Gutiérrez-Salmerón M, Morales V, Chocarro-Calvo A, Sanz R, García-Jiménez C, García-Muñoz RA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Decellularized human umbilical artery: Biocompatibility and in vivo functionality in sheep carotid bypass model.
en DC PMID: 32409090 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Fang S, Riber SS, Hussein K, Ahlmann AH, Harvald EB, Khan F, Beck HC, Weile LKK, Sørensen JA, Sheikh SP, Riber LP, Andersen DC Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Development of GelMA/PCL and dECM/PCL resins for 3D printing of acellular in vitro tissue scaffolds by stereolithography.
Abstract Gelatin methacryloyl (GelMA) is a chemically modified extracellular matrix (ECM)-derived biopolymer that is widely used for 3D fabrication of tissue engineering scaffolds. However, its tendency for physical gelation limits its use in aqueous 3D printing resins to low concentrations, yielding a poor printing resolution in stereolithography (SLA). To obtain a GelMA-based resin that can be printed into high-resolution tissue scaffolds, we formulated resins of fish and porcine-derived GelMA in formamide using GelMA alone or mixed with star-shaped poly(ε-caprolactone) methacrylate (PCL-MA). We identifi...
Source: Appl Human Sci - May 16, 2020 Category: Physiology Authors: Elomaa L, Keshi E, Sauer IM, Weinhart M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Green fabrication of 3D hierarchical blossom-like hybrid of peeled montmorillonite-ZnO for in-vitro electrochemical sensing of diltiazem hydrochloride drug.
Abstract Herein, a 3D hierarchical blossom-like of Montmorillonite-ZnO (MMt/ZnO) micro-hybrids modified sensors have been successfully fabricated as an extraordinarily electrochemical sensor for detecting of the Diltiazem hydrochloride (DZM.HCl). The 3D hierarchical blossom-like of ZnO and series of MMt/ZnO hybrids have been synthesized using different contents of MMt [FMZ1-5] via a hydrogel polymer template method using alginate ions. The effect of incorporation of different contents of MMt on the morphology, surface area of hybrids were investigated using Fourier transform infrared (FTIR), X-ray diffraction (XRD...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Elfiky M, Salahuddin N, Matsuda A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Quaternary ammonium silane, calcium and phosphorus-loaded PLGA submicron particles against Enterococcus faecalis infection of teeth: An in vitro and in vivo study.
Abstract Refractory root canal infection of human teeth is the primary cause of dental treatment failure. Enterococcus faecalis is the major cause of refractory root canal infection. In the present study, poly(D,L-lactic-co-glycolide) (PLGA) submicron particles were used as carriers to deliver an antimicrobial quaternary ammonium silane (code-named K21) as well as calcium and phosphorus elements. The release profiles, antibacterial ability against E. faecalis, extent of infiltration into dentinal tubules, biocompatibility and in vitro mineralization potential of the particles were investigated. In addition, the an...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Fan W, Li Y, Sun Q, Tay FR, Fan B Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Cell-free scaffold from jellyfish Cassiopea andromeda (Cnidaria; Scyphozoa) for skin tissue engineering.
iacute;guez JV, Castaño-Meneses VM Abstract Disruption of the continuous cutaneous membrane in the integumentary system is considered a health problem of high cost for any nation. Several attempts have been made for developing skin substitutes in order to restore injured tissue including autologous implants and the use of scaffolds based on synthetic and natural materials. Current biomaterials used for skin tissue repair include several scaffold matrices types, synthetic or natural, absorbable, degradable or non-degradable polymers, porous or dense scaffolds, and cells capsulated in hydrogels or spheroids s...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Fernández-Cervantes I, Rodríguez-Fuentes N, León-Deniz LV, Alcántara Quintana LE, Cervantes-Uc JM, Herrera Kao WA, Cerón-Espinosa JD, Cauich-Rodríguez JV, Castaño-Meneses VM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A novel fluorescent hydroxyapatite based on iron quantum cluster template to enhance osteogenic differentiation.
Abstract Template-mediated self-assembly synthesis has produced a diverse range of biomimetic materials with unique physicochemical properties. Here, we fabricated novel fluorescent three-dimensional (3-D) hydroxyapatite (HAP) nanorod-assembled microspheres using iron quantum cluster (FeQC) as a hybrid template, containing three organic components: hemoglobin chains, piperidine, and iron clusters. The material characterization indicated that the synthesized HAP possessed a uniform rod-like morphology, ordered 3-D architecture, high crystallinity, self-activated fluorescence, and remarkable photostability. Our stud...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Hashemi N, Vaezi Z, Khanmohammadi S, Naderi Sohi A, Masoumi S, Hruschka V, Wolbank S, Redl H, Marolt Presen D, Naderi-Manesh H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Bactericidal and antifouling electrospun PVA nanofibers modified with a quaternary ammonium salt and zwitterionic sulfopropylbetaine.
Abstract Bacterial adhesion and colonization on material surfaces have attracted great attention due to their potential threat to human health. Combining bactericidal and antifouling functions has been confirmed as an optimal strategy to prevent microbial infection. In this work, biodegradable electrospun polyvinyl alcohol (PVA) nanofibers were chosen due to its high specific area and abundant reactive hydroxyl groups. A quaternary ammonium salt (IQAS) and zwitterionic sulfopropylbetaine (ISB), both containing isocyanate (NCO) groups, were chemically bonded to the PVA nanofiber surface via a coupling reaction betw...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Zhang T, Gu J, Liu X, Wei D, Zhou H, Xiao H, Zhang Z, Yu H, Chen S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A human epidermal growth factor-curcumin bandage bioconjugate loaded with mesenchymal stem cell for in vivo diabetic wound healing.
In conclusion, biocompatible therapeutic MSCs-EGF-Cur B might have great application for diabetic wound healing in the near future. PMID: 32279771 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Mohanty C, Pradhan J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hydrogel formation by short D-peptide for cell-culture scaffolds.
Abstract The present study reports that a short oligopeptide D-P1, consisting of only five D-amino acids, self-assembled into entangled nanofibers to form a hydrogel that functioned as a scaffold for cell cultures. D-P1 (Ac-D-Phe-D-Phe-D-Phe-Gly-D-Lys) gelated aqueous buffer solution and water at a minimum gelation concentration of 0.5 wt%. The circular dichroism (CD) measurements demonstrated the formation of a β-sheet structure in the self-assembly of D-P1. We investigated the gelation properties and CD spectra of both the D- and L-forms of the oligopeptide, and found only a minimal difference between ...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Restu WK, Yamamoto S, Nishida Y, Ienaga H, Aoi T, Maruyama T Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Effect of added porosity on a novel porous Ti-Nb-Ta-Fe-Mn alloy exposed to simulated body fluid.
, Aguilar C Abstract Porous titanium materials have gained interest as prosthesis materials due to their similar mechanical properties to the human bone, biocompatibility, and high corrosion resistance. The presence of pores in the metal matrix implies a decrease in the elastic modulus and an increase in the active area, perhaps improving the osseointegration. Corrosion resistance is a critical consideration as corrosion may lead not only to mechanical failure but also the release of ions and/or particles to the bloodstream. In this work, a novel Ti-Nb-Ta-Fe-Mn alloy with varying percentage of porosity (25, 31 and...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Guerra C, Sancy M, Walczak M, Martínez C, Ringuedé A, Cassir M, Han J, Ogle K, de Melo HG, Salinas V, Aguilar C Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Effects of macropore size in carbonate apatite honeycomb scaffolds on bone regeneration.
Abstract The pore architecture of scaffolds is a critical factor for angiogenesis and bone regeneration. Although the effects of scaffold macropore size have been investigated, most scaffolds feature macropores with poor uniformity and interconnectivity, and other parameters (e.g., microporosity, chemical composition, and strut thickness) differ among scaffolds. To clarify the threshold of effective macropore size, we fabricated honeycomb scaffolds (HCSs) with distinct macropore (i.e., channel) sizes (~100, ~200, and ~300 μm). The HCSs were composed of AB-type carbonate apatite with ~8.5% carbonate ions, i...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Hayashi K, Munar ML, Ishikawa K Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Integrating cold atmospheric plasma with 3D printed bioactive nanocomposite scaffold for cartilage regeneration.
In this study, a novel biomimetic cartilage scaffold was developed by integrating a cold atmospheric plasma (CAP) treatment with prolonged release of bioactive factors. Specifically, a surface of 3D printed hydrogel scaffold with drug-loaded nanoparticles was treated with CAP. Our results showed that the scaffolds with CAP treatment can improve hydrophilicity as well as surface nano-roughness and can thus facilitate stem cell adhesion. More importantly, this study demonstrated that integrating CAP treatment with drug-loaded nanoparticles can synergistically enhance chondrogenesis of human bone marrow mesenchymal stem cells...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Lee SJ, Yan D, Zhou X, Cui H, Esworthy T, Hann SY, Keidar M, Zhang LG Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Combinatory interpretation of protein corona and shear stress for active cancer targeting of bioorthogonally clickable gelatin-oleic nanoparticles.
Abstract Nanoparticle-protein interactions under conditions mimicking physiology determine how nanoparticles (NPs) will behave inside blood vessels and, therefore, the overall outcome of the drug-delivery system. Here, for the first time, we explore the effects of bio-mimicking shear stress and protein corona conditions on novel active targeting of clickable fattigation nanoparticles (NPs) for cancer therapy. Active targeting dibenzocyclooctyne-functionalized biocompatible gelatin-oleic NPs (GON-DBCOs) via a bioorthogonal click reaction were prepared by the desolvation method for delivery of docetaxel (DTX) to lun...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Meghani NM, Amin H, Park C, Cui JH, Cao QR, Choi KH, Lee BJ Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Graphitic carbon nitride nanosheets prepared by electrophoretic size fractionation as an anticancer agent against human bone carcinoma.
Abstract Graphitic carbon nitride, g-C3N4, is a fascinating candidate for biomedical applications. Of course, bulk g-C3N4 is not appropriate for this purpose due to its large size distribution and low dispersion in water. Herein, for the first time, the electrophoretic size fractionation of g-C3N4 without introducing some functional groups into its structure was performed within a very short time. This simple separation technique resulted in several factions. The smallest collected fraction was nanosheets and showed the enhanced photoluminescence properties such as high PL intensity and bright luminescence. The na...
Source: Appl Human Sci - April 15, 2020 Category: Physiology Authors: Davardoostmanesh M, Ahmadzadeh H, Goharshadi EK, Meshkini A, Sistanipour E Tags: Mater Sci Eng C Mater Biol Appl Source Type: research