Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF.
Abstract In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF). The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters. The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation. Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Park KH, Kim SJ, Jeong YH, Moon HJ, Song HJ, Park YJ Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Crystal structure, optical properties, DFT analysis of new morpholine based Schiff base ligands and their copper(II) complexes: DNA, protein docking analyses, antibacterial study and anticancer evaluation.
Abstract New morpholine derived Schiff base ligands (HL1 and HL2) and their Cu(II) complexes [Cu(L1)2] (1) and [Cu(L2)2] (2) have been synthesized and characterized by 1H NMR, IR, UV-Vis, EPR studies and cyclic voltammetric analyses. Single crystal X-ray crystallography studies confirm the structure of newly synthesized Schiff base ligands HL1and HL2. The ground state electronic structures of Cu(II) complexes 1 and 2 have been investigated by DFT/B3LYP theoretical analysis with 6-31G (d,p) and LANL2DZ basis set. The affinity towards DNA and protein molecules have been evaluated using computational docking analysis...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Dhahagani K, Kesavan MP, Gujuluva Gangatharan Vinoth K, Ravi L, Rajagopal G, Rajesh J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation, characterization and application research of a sustained dexamethasone releasing electrode coating for cochlear implantation.
Abstract Cochlear inflammatory response after cochlear implantation (CI) is an important mechanism for implantation trauma and hearing loss. The hearing loss was also caused by damage to auditory hair cells (HCs), whereas ion homeostasis within the cochlea can ensure survival of HCs. In our study, pure hyaluronic acid (HA) was crosslinked with 1, 4-butanediol diglycidyl ether (BDDE) and the successful preparation of the cross-linked hydrogel (CHA) was confirmed by rheological characteristics and FTIR spectra. Artificial perilymph (APL) was prepared to simulate the ion homeostasis microenvironment within scala tymp...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Xu M, Ma D, Chen D, Cai J, He Q, Shu F, Tang J, Zhang H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Copper-modified Ti6Al4V alloy fabricated by selective laser melting with pro-angiogenic and anti-inflammatory properties for potential guided bone regeneration applications.
Abstract Custom-made biocompatible titanium alloy mesh can be designed to facilitate the regeneration of alveolar bone defects by supporting a protected space and inhibiting bacterial infections. Copper ions are often incorporated into titanium alloy due to their high bioactivity and outstanding antibacterial properties. However, the impacts of copper-bearing alloys on peri-implanted cell behaviors have rarely been systematically explored. In the present study, a copper-bearing alloy (Ti6Al4V-6Cu) was fabricated by selective laser melting (SLM) technology. The characterization of Ti6Al4V-6Cu alloy and its effects ...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Xu X, Lu Y, Li S, Guo S, He M, Luo K, Lin J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Efficient synthesis of PMMA@Co0.5Ni0.5Fe2O4 organic-inorganic hybrids containing hyamine 1622 - Physicochemical properties, cytotoxic assessment and antimicrobial activity.
Abstract The PMMA@Co0.5Ni0.5Fe2O4 ferrite containing hybrid nanomaterials with hyamine were prepared using emulsion polymerization method. Structural and morphological properties were evaluated using XRD, FT-IR, SEM techniques. The TGA and DTA analysis were performed in order to study the thermal properties of hybrid materials in contrast to reference material. Magnetic properties were studied using Quantum Design PPMS (VSM option) in a constant external magnetic field equal (100 Oe and 1000 Oe) in the temperature range from 2 to 380 K. Both the pure Co0.5Ni0.5Fe2O4and the sample with 85% of PMMA exhibit sup...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Zachanowicz E, Pigłowski J, Grzymajło M, Poźniak B, Tikhomirov M, Pierunek N, Śniadecki Z, Idzikowski B, Marycz K, Marędziak M, Kisała J, Hęclik K, Pązik R Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Investigation on the properties of borate bonding agents: Ti6Al4V-porcelain bonding, chemical durability and preliminary cytotoxicity.
Abstract The purpose of this study was to investigate the properties of the borate bonding agents (BBAS) including chemical durability, biocompatibility and bonding characteristics of porcelain to Ti6Al4V. The bond strength was performed by the three-point bending test. And the chemical durability and ion release of BBAS were tested by chemical soaking and inductively coupled plasma optical emission spectrometry (ICP-OES), respectively. Moreover, cytotoxicity was evaluated by cell viability assay and cell adhesion using human osteosarcoma cells (MG-63) and cell counter kit-8 (CCK-8) assay. To investigate the influ...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Zhao CQ, Guo S, Wu SQ, Luo JS, Lu YJ, Huang TT, Lin JX Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites.
Abstract To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated. Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated. The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Zhao M, Dai Y, Li X, Li Y, Zhang Y, Wu H, Wen Z, Dai C Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Potential of silk sericin based nanofibrous mats for wound dressing applications.
Abstract Wound dressing developed using bioactive materials has been a current area of research for treating chronic non-healing wounds owing to its high demand. Here, we report the fabrication and evaluation of nanofibrous matrix based wound dressings using biopolymer poly(vinyl alcohol) (PVA) incorporated with silk sericin (SS). SS extracted from the cocoons of mulberry variety Bombyx mori and non-mulberry variety Antheraea assama has been used to develop two types of blended mats. Herein, SS based nanofibrous dressings fabricated using electrospinning technique were thoroughly characterized and evaluated for wo...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Gilotra S, Chouhan D, Bhardwaj N, Nandi SK, Mandal BB Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation and mechanistic aspect of natural xanthone functionalized gold nanoparticle.
Abstract Herein, a facile scale up and shape variable synthesis of gold nanoparticle (AuNP) and reaction mechanism by natural xanthone derivative (mangiferin) has been reported. Mangiferin (C19H18O11; 1,3,6,7-tetrahydroxyxanthone-C2-β-d-glucoside), a xanthone derivative is isolated from Mangifera indica L. leaves which efficiently reduces Au3+ ions to Au0 and stabilizes the formed AuNP. The structural, optical and plasmonic properties of synthesized AuNP have been investigated through different instrumental techniques like UV-Vis and FTIR spectroscopy, powder XRD, FESEM and TEM analysis. It is observed that v...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Patra N, Dehury N, Pal A, Behera A, Patra S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.
Abstract Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique. The composite was characterized by using FT-IR, XRD, HRTEM, SAED, EDS, and SEM. TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm. Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL). The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 μg/ml to 200 μg/ml). ELISA ...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Hira I, Kumar A, Kumari R, Saini AK, Saini RV Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Green approach for in-situ growth of highly-ordered 3D flower-like CuS hollow nanospheres decorated on nitrogen and sulfur co-doped graphene bionanocomposite with enhanced peroxidase-like catalytic activity performance for colorimetric biosensing of glucose.
We describe a green and facile hydrothermal route to synthesize highly ordered 3D flower-like hierarchical CuS hollow nanospheres@N-S-rGO bionanocomposite used to detect glucose. After synthesis and comprehensive characterization of all potential catalysts, the peroxidase-like activity of 3D CuS NSs@N-S-rGO is compared extensively with pure 3D CuS NSs, N-S-rGO, N-rGO, and GO to monitor the effect of each modification and treatment on their catalytic properties. The results indicate promising peroxidase activity of 3D CuS NSs@N-S-rGO by possessing the highest catalytic activity and the Michaelis constant of ~93.5 times lowe...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Nekouei F, Nekouei S, Jashnsaz O, Pouzesh M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Polymer coating on a micropillar chip for robust attachment of PuraMatrix peptide hydrogel for 3D hepatic cell culture.
In this study, we have investigated several polymer coating materials to robustly attach PuraMatrix peptide hydrogel on a micropillar chip for 3D culture of Hep3B human hepatic cells, which can be used as a tool for high-throughput assessment of compound hepatotoxicity. Among several amphiphilic polymers with maleic anhydride groups tested, 0.01% (w/v) poly(maleic anhydride-alt-1-octadecene) (PMA-OD) provided superior coating properties with no PuraMatrix spot detachment from the micropillar chip and no air bubble entrapment in a complementary microwell chip. To maintain Hep3B cell viability in PuraMatrix gel on the chip, ...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Roth AD, Lama P, Dunn S, Hong S, Lee MY Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Injectable synthetic hydrogel for bone regeneration: Physicochemical characterisation of a high and a low pH gelling system.
Abstract Hybrid poly(ethylene glycol)-co-peptide hydrogels are a versatile platform for bone regeneration. For the use as injectable scaffolds, a good understanding of reaction kinetics and physical properties is vital. However, these factors have not yet been comprehensively illuminated. We show that gelation time can be effectively controlled by pH without affecting the elasticity of the formed hydrogels. Maleimide functionalised PEG gels at lower pH and produces more densely cross-linked networks than vinylsulfone functionalised PEG. Both form non-ideal networks. The elastic moduli on the order of a few kPa are...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Schweikle M, Zinn T, Lund R, Tiainen H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Do physico-chemical properties of silver nanoparticles decide their interaction with biological media and bactericidal action? A review.
Abstract The unprecedented increase in antibiotic resistance in this era has resuscitated the attention of scientific community to exploit silver and its various species as antimicrobial agents. Plenty of studies have been done to measure the antimicrobial potential of silver species (cationic silver, metallic Ag0 or silver nanoparticles, silver oxide particulates etc.) and indicated that membrane damage, oxidative stress, protein dysfunction and DNA damage to be the possible cause of injury to the microbial cell. However, the precise molecular mechanism of their mode of action has remained unclear, which makes an...
Source: Appl Human Sci - June 2, 2018 Category: Physiology Authors: Pareek V, Gupta R, Panwar J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Gemcitabine-loaded gold nanospheres mediated by albumin for enhanced anti-tumor activity combining with CT imaging.
Abstract Currently, the development of a more effective therapy approach overcoming the resistance to chemotherapy for lung cancer still faces great challenges and requires further investigation. Herein, we fabricated gemcitabine (Gem) loaded gold nanospheres mediated by bovine serum albumin (Au@BSA) for the efficient delivery of therapeutic agents into the human pulmonary carcinoma cells (A549) and simultaneous computed tomography (CT) imaging. These nanoparticles (Au@BSA) with an approximate diameter of 10 nm were single crystals and prepared through a protein-directed facile one-pot synthesis via the reductio...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Wang Z, Chen L, Chu Z, Huang C, Huang Y, Jia N Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Effect of Polyhydroxybutyrate/Chitosan/Bioglass nanofiber scaffold on proliferation and differentiation of stem cells from human exfoliated deciduous teeth into odontoblast-like cells.
Abstract Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazoliu...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Khoroushi M, Foroughi MR, Karbasi S, Hashemibeni B, Khademi AA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

How cell culture conditions affect the microstructure and nanomechanical properties of extracellular matrix formed by immortalized human mesenchymal stem cells: An experimental and modelling study.
Abstract This paper presents an investigation of how different culture media (i.e. basal and osteogenic media) affect the nanomechanical properties and microstructure of the mineralized matrix produced by the human mesenchymal stem cell line Y201, from both an experimental and theoretical approach. A bone nodule (i.e. mineralized matrix) cultured from basal medium shows a more anisotropic microstructure compared to its counterpart cultured from an osteogenic medium. As confirmed by finite element simulations, this anisotropic microstructure explains the bimodal distribution of the corresponding mechanical properti...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Duan P, Toumpaniari R, Partridge S, Birch MA, Genever PG, Bull SJ, Dalgarno KW, McCaskie AW, Chen J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

hucMSC derived exosomes promote functional recovery in spinal cord injury mice via attenuating inflammation.
Abstract The exploration of effective spinal cord injury (SCI) healing still remain a great challenge due to the high morbidity, complex pathology and unclear targets. Human umbilical cord mesenchymal stem cells (hucMSC) play an important role in tissue regeneration. However, transplanting stem cells has a potential risk of teratogenicity. Recent studies have suggested that exosomes secreted by stem cells may contribute to tissue injury repair. We hypothesized that the application of hucMSC derived exosomes may be a potential way for SCI treatment. Our studies showed the hucMSC derived exosomes with a mean particl...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Sun G, Li G, Li D, Huang W, Zhang R, Zhang H, Duan Y, Wang B Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Photo/pH-controlled host-guest interaction between an azobenzene-containing block copolymer and water-soluble pillar[6]arene as a strategy to construct the "compound vesicles" for controlled drug delivery.
Photo/pH-controlled host-guest interaction between an azobenzene-containing block copolymer and water-soluble pillar[6]arene as a strategy to construct the "compound vesicles" for controlled drug delivery. Mater Sci Eng C Mater Biol Appl. 2018 Aug 01;89:237-244 Authors: Zhou J, Xu H, Tong Z, Yang Y, Jiang G Abstract Herein, dual stimuli-responsive compound vesicles were constructed based on host-guest interaction between a water-soluble pillar[6]arene (WP6) and an amphiphilic azobenzene-containing block copolymers (BCP). Reversible morphological transformation between compound vesicles and s...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Zhou J, Xu H, Tong Z, Yang Y, Jiang G Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Silk fibroin/nanohydroxyapatite hydrogels for promoted bioactivity and osteoblastic proliferation and differentiation of human bone marrow stromal cells.
Abstract Silk fibroin (SF) is a natural, biocompatible, and biodegradable polymer having a great potential for the successful regeneration of damaged bone tissue. In the present work, nanohydroxyapatite (nanoHA) was incorporated into SF polymer to form a bioactive composite hydrogel for applications as bone implants. The degradation and bioactive properties of SF/nanoHA composite hydrogels were evaluated. Additionally, biological investigations of human bone marrow stromal cells (hBMSCs) viability, proliferation and differentiation to the osteoblastic phenotype were conducted. The incorporation of nanoHA in SF pol...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Ribeiro M, Fernandes MH, Beppu MM, Monteiro FJ, Ferraz MP Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites.
In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on ...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Ignjatović NL, Penov-Gaši KM, Ajduković JJ, Kojić VV, Marković SB, Uskoković DP Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Antimicrobial and anticancer activities of silver nanoparticles synthesized from the root hair extract of Phoenix dactylifera.
Abstract There is a continuous rise in the rate of medicine consumption because of the development of drug resistance by microbial pathogens. In the last one decade, silver nanoparticles (AgNPs) have become a remarkable choice for the development of new drugs due to their excellent broad-spectrum antimicrobial activity. In the current piece of work, we have synthesized AgNPs from the root extract of Phoenix dactylifera to test their antimicrobial and anti-cancer potential. UV-visible spectra showed the surface plasmon resonance peak at 420 nm λmax corresponding to the formation of silver nanoparticles, FT...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Oves M, Aslam M, Rauf MA, Qayyum S, Qari HA, Khan MS, Alam MZ, Tabrez S, Pugazhendhi A, Ismail IMI Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Strontium-modified chitosan/montmorillonite composites as bone tissue engineering scaffold.
The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications. With the freeze-drying technique, strontium (Sr2+) modified C/MMT composite scaffold with an interconnected porous structure was produced. X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold. One of the aims of this study was to document the release of Sr2+ from the non-mo...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Koç Demir A, Elçin AE, Elçin YM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synthesis and characterization of kaempferol-based ruthenium (II) complex: A facile approach for superior anticancer application.
In this study, we synthesized a novel metal flavonoid complex and investigated its effects on the non-small cell lung cancer cell lines, A549 and toxicity on the human dermal fibroblast cell lines, HDFa. 1H, 13C NMR, single crystal X-ray diffraction and elemental micro analysis (C,H,N,S/O) were used to characterize the synthesized kaempferol-based Ru (II) complex. Cell toxicity was studied using MTT assay and electric cell substrate impedance sensing (ECIS). It was evident from the MTT results that no significant cytotoxicity in HDFa cells occurs with the synthesized complex, but in case of A549 cells, significant cytotoxi...
Source: Appl Human Sci - May 13, 2018 Category: Physiology Authors: Thangavel P, Viswanath B, Kim S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Elucidating molecular events underlying topography mediated cardiomyogenesis of stem cells on 3D nanofibrous scaffolds.
This study demonstrates that aligned nanofibrous scaffolds augment cardiomyogenic differentiation wherein topography plays a critical role in driving stem cell function. In addition, this study offers insight into molecular pathways driving the cellular response. PMID: 29636125 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - April 12, 2018 Category: Physiology Authors: Ghosh LD, Jain A, Sundaresan NR, Chatterjee K Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

One-step fabrication of functionalized poly(etheretherketone) surfaces with enhanced biocompatibility and osteogenic activity.
Abstract Polyetheretherketone (PEEK) has an elastic modulus similar to that of the bone; however, its use as a material for bone repair is limited by bio-inert surface chemistry and poor osteogenesis-inducing capacity. To address this issue, the PEEK surface was activated by ultraviolet radiation-induced grafting of methacrylated hyaluronic acid (MeHA) and titanium dioxide (TiO2) nanofibers via a one-step process. The modified PEEK surface was characterized by X-ray photoelectron and Fourier-transform infrared spectroscopy, and the extent of surface modification was evaluated by measuring static contact angles. At...
Source: Appl Human Sci - April 12, 2018 Category: Physiology Authors: Liu S, Zhu Y, Gao H, Ge P, Ren K, Gao J, Cao Y, Han D, Zhang J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Functionalized coatings by cold spray: An in vitro study of micro- and nanocrystalline hydroxyapatite compared to porous titanium.
ny JM Abstract Three different surface treatments on a Ti6Al4V alloy have been in vitro tested for possible application in cementless joint prosthesis. All of them involve the novelty of using the Cold Spray technology for their deposition: (i) an as-sprayed highly rough titanium and, followed by the deposition of a thin hydroxyapatite layer with (ii) microcrystalline or (iii) nanocrystalline structure. Primary human osteoblasts were extracted from knee and seeded onto the three different surfaces. Cell viability was tested by MTS and LIVE/DEAD assays, cell differentiation by alkaline phosphatase (ALP) quantificat...
Source: Appl Human Sci - March 24, 2018 Category: Physiology Authors: Vilardell AM, Cinca N, Garcia-Giralt N, Dosta S, Cano IG, Nogués X, Guilemany JM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Formation and in vitro/in vivo performance of "cortex-like" micro/nano-structured TiO2 coatings on titanium by micro-arc oxidation.
Formation and in vitro/in vivo performance of "cortex-like" micro/nano-structured TiO2 coatings on titanium by micro-arc oxidation. Mater Sci Eng C Mater Biol Appl. 2018 Jun 01;87:90-103 Authors: Li Y, Wang W, Liu H, Lei J, Zhang J, Zhou H, Qi M Abstract A novel "cortex-like" micro/nano dual-scale structured TiO2 coating was prepared on a titanium surface by micro-arc oxidation (MAO) with tetraborate electrolytes. This structure, which combines microslots with nanopores, exhibits super hydrophilicity. This coating modified the surface structure, chemistry, and hydrophilicity in a o...
Source: Appl Human Sci - March 24, 2018 Category: Physiology Authors: Li Y, Wang W, Liu H, Lei J, Zhang J, Zhou H, Qi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation and characterization of gelatin-chitosan-nano β-TCP based scaffold for orthopaedic application.
Preparation and characterization of gelatin-chitosan-nanoβ-TCP based scaffold for orthopaedic application. Mater Sci Eng C Mater Biol Appl. 2018 May 01;86:83-94 Authors: Maji K, Dasgupta S, Pramanik K, Bissoyi A Abstract The primary aim of this study was to fabricate gelatin/chitosan/β-TCP (GCT) composite scaffold to improve its compressive mechanical behaviour and in-vivo biocompatibility with predictable degradation rate. Beta tricalcium phosphate (β-TCP) powder was synthesized in size range between 70-100 nm using aqueous precipitation route at a fixed Ca/P molar ratio of 1.5:1 at ...
Source: Appl Human Sci - March 13, 2018 Category: Physiology Authors: Maji K, Dasgupta S, Pramanik K, Bissoyi A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Human amnion extracellular matrix derived bioactive hydrogel for cell delivery and tissue engineering.
PMID: 29407148 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - February 8, 2018 Category: Physiology Authors: Ryzhuk V, Zeng XX, Wang X, Melnychuk V, Lankford L, Farmer D, Wang A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Chlorophyll a in cyclodextrin supramolecular complexes as a natural photosensitizer for photodynamic therapy (PDT) applications.
Abstract Chlorophyll a (Chl a), an amphipathic porphyrin, was employed as natural photosensitizer for photodynamic therapy applications. Due to its lacking solubility in water and high tendency to aggregate, Chl a was included into different modified cyclodextrins (CDs) to form stable water-soluble supramolecular complexes. To achieve this aim, 2-Hydroxypropyl-β-cyclodextrin (2-HP-β-CD), 2-Hydroxypropyl-γ-cyclodextrin (2-HP-γ-CD), Heptakis(2,6-di-o-methyl)-β-cyclodextrin (DIMEB) and Heptakis(2,3,6-tri-o-methyl)-β-cyclodextrin (TRIMEB) were used. The chemical physical properties of C...
Source: Appl Human Sci - February 8, 2018 Category: Physiology Authors: Semeraro P, Chimienti G, Altamura E, Fini P, Rizzi V, Cosma P Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects.
ellomäki M Abstract Corneal blindness is a worldwide problem, plagued by insufficient amount of high-quality donor tissue. Cell therapy using human adipose stem cells (hASCs) has risen as an alternative to regenerate damaged corneal stromal tissue, the main structural and refractive layer of the cornea. Herein we propose a method to deliver hASCs into corneal defects in hyaluronan (HA)-based hydrogels, which form rapidly in situ by hydrazone crosslinking. We fabricated two different HA-based hydrazone-crosslinked hydrogels (HALD1-HACDH and HALD2-HAADH), and characterized their swelling, degradation, mechanica...
Source: Appl Human Sci - February 8, 2018 Category: Physiology Authors: Koivusalo L, Karvinen J, Sorsa E, Jönkkäri I, Väliaho J, Kallio P, Ilmarinen T, Miettinen S, Skottman H, Kellomäki M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Behavioral and hematological responses of broiler chickens administered with betaine and ascorbic acid during hot-dry season.
Abstract Heat stress is a major problem in poultry production in tropical regions. Assessing the impact of thermally stressful environmental conditions on the welfare of broiler chickens is of great importance. Behavioral responses in a novel environment and hematology of broiler chickens administered with betaine and/or ascorbic acid (AA) during the hot-dry season were evaluated. Broiler chickens were randomly divided into four groups: Group I (control) was given sterile water, Group II was given betaine, Group III was given AA, and Group IV received betaine + AA orally and daily for 42 days. An open-field t...
Source: Appl Human Sci - February 5, 2018 Category: Physiology Authors: Egbuniwe IC, Ayo JO, Kawu MU, Mohammed A Tags: J Appl Anim Welf Sci Source Type: research

Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth.
ai VT Abstract The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and BiodentineTM (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. SHED were cultured for 1 - 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 μm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by re...
Source: Appl Human Sci - February 1, 2018 Category: Physiology Authors: Araújo LB, Cosme-Silva L, Fernandes AP, Oliveira TM, Cavalcanti BDN, Gomes Filho JE, Sakai VT Tags: J Appl Oral Sci Source Type: research

Biosynthesis of cerium oxide nanoparticles and their effect on lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction in male Sprague Dawley rats.
Abstract The fetal/life challenging Sepsis is an urgent medical disaster which have developed "blood poisoning". The sepsis is one of the disorders of organs which cause death in patients. The investigational models that mimicchemical change of sepsis in human bodywereessential. We have synthesized CeO2 NPs using eco-friendly method using plant extract. The characterization of synthesized CeO2 NPs was studied using UV-Vis, FTIR, XRD, SEM, EDX and TEM analysis. The results were confirmed that the synthesized CeO2 was in nano size with spherical shape. The synthesized CeO2 nanoparticles were evaluated agai...
Source: Appl Human Sci - December 7, 2017 Category: Physiology Authors: Chen G, Xu Y Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hemostatic porous sponges of cross-linked hyaluronic acid/cationized dextran by one self-foaming process.
Abstract Effective hemostatic materials are very important for treating trauma cases. Natural polysaccharides have been particularly appealing in the development of new hemostatic materials due to their unique functions in human bodies. In this work, different polysaccharide-based hemostatic porous sponges (SHDP or SHDQ) of cross-linked hyaluronic acid (HA)/cationized dextran were readily prepared by the self-foaming process of HA and poly((2-dimethyl amino)-ethyl methacrylate)-grafted dextran (Dex-PDM) or partially-quaternized Dex-PDM in the presence of sodium trimetaphosphate crosslinkers. SHDP and SHDQ sponges ...
Source: Appl Human Sci - December 7, 2017 Category: Physiology Authors: Liu JY, Li Y, Hu Y, Cheng G, Ye E, Shen C, Xu FJ Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synergetically understanding the interaction between nano/microspheres and peptide for controllable drug loading via experimental and theoretical approaches.
Abstract In this paper we systematically investigate the loading capacity of raspberry-like nano/microspheres with highly cross-linked structure for the peptide, immunostimulating hexapeptide from human (IHH), by integrating both experimental and simulation efforts. The experimental results indicate that the loading capacities of raspberry-like nano/microspheres with different functionalized chains vary drastically. To provide theoretical insights into the observed phenomenon, the typical raspberry-like nano/microspheres were simplified as effective functionalized groups, thereby the interactions between them and ...
Source: Appl Human Sci - December 7, 2017 Category: Physiology Authors: Du C, Hu X, Cheng Y, Gao J, Zhang YW, Su K, Li Z, Zhang N, Chang N, Zeng K Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preliminary investigation of the effect of doping of copper oxide in CaO-SiO2-P2O5-MgO bioactive composition for bone repair applications.
Abstract A diopside based bioactive system with a nominal composition of xCuO-(45.55-x)CaO-29.44 SiO2-10.28P2O5-14.73 MgO (x=0,1,3 and 5mol%) has been prepared by sol gel technique in the laboratory. X-ray Diffraction, Fourier Transform Infra-Red and Raman Spectroscopy, Field Emission Scanning Electron Microscopy along with Energy Dispersive X-ray Analysis and pH studies have been undertaken on the prepared samples before and after dipping the samples in simulated body fluid. It has been observed that hydroxyapatite layer starts to form with in 24h during immersion in simulated body fluid. Degradation studies have...
Source: Appl Human Sci - December 7, 2017 Category: Physiology Authors: Kaur P, Singh KJ, Yadav AK, Sood H, Kaur S, Kaur R, Arora DS, Kaur S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Development of temozolomide coated nano zinc oxide for reversing the resistance of malignant glioma stem cells.
Abstract Recently most of the researchers have turned their interest towards plant mediated synthesis of metal nanoparticles to avoid several environmental toxicants. In this manuscript, we have discussed the ecofriendly syntheses of zinc oxide nanoparticles (ZnO NPs) were achieved using Glycyrrhiza glabra (G. glabra) seed aqueous extract. The green synthesized ZnO NPs were characterized using analytical techniques like XRD, TEM, particle size histogram and Zeta potential. From the results, it was found that the green synthesized ZnO NPs were around 35nm in size with irregular spherical shape. The Zeta potential s...
Source: Appl Human Sci - December 7, 2017 Category: Physiology Authors: Zheng M, Wang S, Liu Z, Xie L, Deng Y Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Effects of copper nanoparticles in porous TiO2 coatings on bacterial resistance and cytocompatibility of osteoblasts and endothelial cells.
In this study, porous TiO2 coatings doped with different amounts of Cu NPs (designated as 0 Cu, 0.3 Cu, and 3.0 Cu) are deposited on titanium by micro-arc oxidation (MAO). The Cu NPs coated samples exhibit excellent antibacterial activity against Staphylococcus aureus (S. aureus). In vitro cytocompatibility evaluation discloses that 0 Cu and 0.3 Cu have no toxicity to osteoblasts but 3.0 Cu shows cytotoxicity. 0.3 Cu promotes proliferation and adhesion of osteoblasts and enhances extracellular matrix mineralization (ECM), but has little effects on the alkaline phosphatase activity (ALP) and collagen secretion. Surprisingly...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Zhang X, Li J, Wang X, Wang Y, Hang R, Huang X, Tang B, Chu PK Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fabrication and preliminary study of a biomimetic tri-layer tubular graft based on fibers and fiber yarns for vascular tissue engineering.
Abstract Designing a biomimetic and functional tissue-engineered vascular graft has been urgently needed for repairing and regenerating defected vascular tissues. Utilizing a multi-layered vascular scaffold is commonly considered an effective way, because multi-layered scaffolds can easily simulate the structure and function of natural blood vessels. Herein, we developed a novel tri-layer tubular graft consisted of Poly(L-lactide-co-caprolactone)/collagen (PLCL/COL) fibers and Poly(lactide-co-glycolide)/silk fibroin (PLGA/SF) yarns via a three-step electrospinning method. The tri-layer vascular graft consisted of ...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Wu T, Zhang J, Wang Y, Li D, Sun B, El-Hamshary H, Yin M, Mo X Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineered chimeric peptides with antimicrobial and titanium-binding functions to inhibit biofilm formation on Ti implants.
Abstract Titanium (Ti) implants have been commonly used in oral medicine. However, despite their widespread clinical application, these implants are susceptible to failure induced by microbial infection due to bacterial biofilm formation. Immobilization of chimeric peptides with antibacterial properties on the Ti surface may be a promising antimicrobial approach to inhibit biofilm formation. Here, chimeric peptides were designed by connecting three sequences (hBD-3-1/2/3) derived from human β-defensin-3 (hBD-3) with Ti-binding peptide-l (TBP-l: RKLPDAGPMHTW) via a triple glycine (G) linker to modify Ti surfac...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Geng H, Yuan Y, Adayi A, Zhang X, Song X, Gong L, Zhang X, Gao P Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Dual-delivery of VEGF and NGF by emulsion electrospun nanofibrous scaffold for peripheral nerve regeneration.
In this study, recombinant human vascular endothelial growth factor (VEGF) and recombinant human nerve growth factor (NGF) were loaded on the surface and in the core of emulsion electrospun poly (l-lactic acid) (PLLA) nanofibrous scaffold, respectively. The in vitro studies showed that VEGF and NGF had a sequential release pattern in which most of the VEFG was released in the first few days but the NGF could be continuously released for>1month. The dual-delivery scaffold could enhance the neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) in vitro. Furthermore, this sc...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Xia B, Lv Y Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Novel fluoridated silk fibroin/ TiO2 nanocomposite scaffolds for bone tissue engineering.
Abstract It is known that Fluoride ions strongly affect bone mineralization and formation. In the present study, the engineered bone tissue scaffolds are fabricated using silk fibroin (SF) and flouridated TiO2 nanoparticles. TiO2 nanoparticles are modified by fluoride ions, and different levels (0, 5, 10, 15 and 20wt%) of the fluoridated TiO2 nanoparticles (TiO2-F) were subsequently added to the SF matrix through phase separation method to prepare silk fibroin/flouridated TiO2 nanocomposite scaffolds (SF/TiO2-F). Phase structure, functional groups, morphology and mechanical properties of the obtained scaffolds wer...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Johari N, Madaah Hosseini HR, Samadikuchaksaraei A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Low systemic toxicity nanocarriers fabricated from heparin-mPEG and PAMAM dendrimers for controlled drug release.
In this report, poly(amide amine) (PAMAM) dendrimer and Heparin-grafted-monomethoxy polyethylene glycol (HEP-mPEG) were synthesized and characterized. In aqueous solution, the generation 4 PAMAM dendrimers (G4.0-PAMAM) existed as nanoparticles with particle size of 5.63nm. However, after electrostatic complexation with HEP-mPEG to form a core@shell structure G4.0-PAMAM@HEP-mPEG, the size of nanoparticles was significantly increased (73.82nm). The G4.0-PAMAM@HEP-mPEG nanoparticles showed their ability to effectively encapsulate doxorubicin (DOX) for prolonged and controlled release. The cytocompatibility of G4.0-PAMAM@HEP-m...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Thanh VM, Nguyen TH, Tran TV, Ngoc UP, Ho MN, Nguyen TT, Chau YNT, Le VT, Tran NQ, Nguyen CK, Nguyen DH Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Cell attachment evaluation of the immobilized bioactive peptide on a nanographene oxide composite.
In this study, the first straightforward strategy for the covalent immobilization of the cell-adhesion peptide onto the graphene surface based on the Ugi four-component assembly process (Ugi 4-CAP) will be presented. The modified adhesion motif peptide, as an amine component in the presence of formaldehyde, cyclohexylisocyanide and carboxylated-graphene (G-COOH), was adopted in a four component reaction to fabricate a peptide-graphene (Peptide-G) biomaterial in water as a green solvent at an ambient temperature. The amino functional groups corresponded to the modified adhesion motif peptide and were immobilized onto the gr...
Source: Appl Human Sci - October 15, 2017 Category: Physiology Authors: Adibi-Motlagh B, Lotfi AS, Rezaei A, Hashemi E Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Evaluating adhesion and alignment of dental pulp stem cells to a spider silk substrate for tissue engineering applications.
This study evaluated the response of human DPSCs to spider dragline silk fibers, a potential substrate material for tissue regeneration. The DPSCs' morphology and spread pattern were characterized after these cells were plated onto Nephila clavipes dragline fibers in media. In addition, the responses of two other well established cell lines, osteoblasts (7F2s), and fibroblasts (3T3s), were also studied under identical conditions. The inclusion of 3T3s and 7F2s in this study allowed for both direct comparisons to prior published work and a qualitative comparison to the morphology of the DPSCs. After twelve days, the DPSCs e...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Hafner K, Montag D, Maeser H, Peng C, Marcotte WR, Dean D, Kennedy MS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineered electrospun polycaprolactone (PCL)/octacalcium phosphate (OCP) scaffold for bone tissue engineering.
Abstract The main challenge in bone tissue engineering is to find suitable biological substitutes which act as scaffolds. Hence, in this work, a novel scaffold composed of octacalcium phosphate (OCP) particles fabricated by co-precipitation method and polycaprolactone (PCL) using electrospinning technique was introduced. The electrospun scaffolds were characterized by SEM, FTIR, XRD, DSC and TGA analysis. The mechanical properties of the composite scaffolds including maximum tensile stress, strain at break and Young modulus were measured. The bioactivity of the scaffolds was determined by soaking in simulated body...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Heydari Z, Mohebbi-Kalhori D, Afarani MS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent.
Abstract The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer 'lignin' as the reducing and capping agents. The peak at 406nm (λmax) in the UV-Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with ...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Marulasiddeshwara MB, Dakshayani SS, Sharath Kumar MN, Chethana R, Raghavendra Kumar P, Devaraja S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber.
Abstract Ulcerative colitis (UC) is a main form of inflammatory bowel disease (IBD). Asafoetida (ASF) and turmeric have traditionally been used for the treatment of various inflammatory diseases, including UC, because ASF is rich in sulfur compounds and turmeric contains curcumin (CUR). Turmeric nanofiber (TNF), the modified cell wall component of turmeric is considered to play important role in the human diet, health and can be used as a carrier agent to encapsulate bioactive components. A novel gut health product (GHP) was formulated by encapsulation of ASF and CUR complex onto TNF. The GHP was characterized by ...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Gopi S, Amalraj A, Jude S, Varma K, Sreeraj TR, Haponiuk JT, Thomas S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research