Effects of physical properties of nano-sized hydroxyapatite crystals on cellular toxicity in renal epithelial cells.
Abstract Hydroxyapatite (HAP) is not only a common component of most idiopathic CaOx stones, but also the core of Randall's plaque. HAP is a nest that can induce the formation of Randall's plaques and even kidney stones. We studied the toxic effects and mechanisms of four different types of nano-HAP crystals (H-Sphere, 72.5 nm × 72.5 nm; H-Needle, 37.2 nm × 162.7 nm; H-Rod, 42.3 nm × 115.3 nm; and H-Plate, 145.5 nm × 272.9 nm) on human renal proximal tubular epithelial cells (HK-2). HAP crystals could cause oxidative stress that triggered a series of cell dys...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Rao CY, Sun XY, Ouyang JM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Papaver somniferum L. mediated novel bioinspired lead oxide (PbO) and iron oxide (Fe2O3) nanoparticles: In-vitro biological applications, biocompatibility and their potential towards HepG2 cell line.
Abstract To overcome the disadvantages of chemical and physical methods, phyto-fabricated nanoparticles attained great attention due to their multifarious applications. Here we successfully demonstrated Papaver somniferum L. mediated green synthesis of lead oxide (PbO) and iron oxide (Fe2O3) nanoparticles. Characterization of nanoparticles involved techniques including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and energy dispersive X-ray (EDX) associated with scanning electron microscopy (SEM). XRD analysis confirmed the phase identification and crystalline nature. FTIR analysis conf...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Muhammad W, Khan MA, Nazir M, Siddiquah A, Mushtaq S, Hashmi SS, Abbasi BH Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo.
Abstract Bioactive glasses have potential applications in the field of regenerative medicine due to their bioactivity that permits interaction with both hard and soft tissues. In the same way, mesenchymal stromal cells (MSCs) have been experimentally tested as part of engineered constructs considering their self-renewal and multipotent capacities. However, to design an association, it is crucial to investigate the physical properties of bioglass 45S5, as well as its biocompatibility. Therefore, we investigated the structural short range order of the stoichiometric 45S5, by obtaining its total structure factors (S(...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Rodrigues C, Naasani LIS, Zanatelli C, Paim TC, Azevedo JG, de Lima JC, da Cruz Fernandes M, Buchner S, Wink MR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fabrication of hierarchically porous silk fibroin-bioactive glass composite scaffold via indirect 3D printing: Effect of particle size on physico-mechanical properties and in vitro cellular behavior.
In this study, novel hierarchically porous silk fibroin (SF) and silk fibroin-bioactive glass (SF-BG) composite were fabricated with controlled architecture and interconnected structure, by combining indirect three-dimensional (3D) inkjet printing and freeze-drying methods. Further, the effect of 45S5 Bioactive glass particles of different sizes (
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Bidgoli MR, Alemzadeh I, Tamjid E, Khafaji M, Vossoughi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biocompatibility and biodegradability of filler encapsulated chloroacetated natural rubber/polyvinyl alcohol nanofiber for wound dressing.
Abstract The novel biodegradable films of chloroacetated natural rubber/polyvinyl alcohol (CNR/PVA) (55/45 wt%) non-woven nanofiber films encapsulated with kaolin and starch (2.5 and 5 wt%) were produced successfully by green electrospinning technique. The effect of fillers with different content on the physical, chemical, mechanical, biocompatibility and biodegradation properties of CNR/PVA nanofiber films were investigated. The higher crystallinity obtained in CNR/PVA encapsulate with 2.5 wt% kaolin and nanofibers were formed with the maximum diameter distribution and mean value of 40-160 nm and 94.15 ...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Azarian MH, Boochathum P, Kongsema M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Electrospun biodegradable chitosan based-poly(urethane urea) scaffolds for soft tissue engineering.
Abstract The composition and architecture of a scaffold determine its supportive role in tissue regeneration. In this work, we demonstrate the feasibility of obtaining a porous electrospun fibrous structure from biodegradable polyurethanes (Pus) synthesized using polycaprolactone-diol as soft segment and, as chain extenders, chitosan (CS) and/or dimethylol propionic acid. Fourier transform infrared spectroscopy and proton nuclear magnetic resonance confirmed the syntheses. Fibre mats' properties were analysed and compared with those of solvent cast films. Scanning electron microscopy images of the electrospun scaf...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Vieira T, Carvalho Silva J, Botelho do Rego AM, Borges JP, Henriques C Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Carbon nanotube composite hydrogels for vocal fold tissue engineering: Biocompatibility, rheology, and porosity.
Abstract Porous composite hydrogels were prepared using glycol chitosan as the matrix, glyoxal as the chemical crosslinker, and carbon nanotubes (CNTs) as the fibers. Both carboxylic and hydroxylic functionalized CNTs were used. The homogeneity of CNTs dispersion was evaluated using scanning electron microscopy. Human vocal fold fibroblasts were cultured and encapsulated in the composite hydrogels with different CNT concentrations to quantify cell viability. Rheological tests were performed to determine the gelation time and the storage modulus as a function of CNT concentration. The gelation time tended to decrea...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Ravanbakhsh H, Bao G, Latifi N, Mongeau LG Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Poly( ε-caprolactone)/poly(glycerol sebacate) electrospun scaffolds for cardiac tissue engineering using benign solvents.
Poly(ε-caprolactone)/poly(glycerol sebacate) electrospun scaffolds for cardiac tissue engineering using benign solvents. Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109712 Authors: Vogt L, Rivera LR, Liverani L, Piegat A, El Fray M, Boccaccini AR Abstract Electrospun fibers based on combinations of poly(ε-caprolactone) (PCL) and poly(glycerol sebacate) (PGS) have been studied for applications in cardiac tissue engineering. The aim of the present study is to demonstrate the fabrication of PCL and PGS prepolymer or mildly crosslinked PGS by electrospinning using less toxic solvents, li...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Vogt L, Rivera LR, Liverani L, Piegat A, El Fray M, Boccaccini AR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Characterization of polycaprolactone/rGO nanocomposite scaffolds obtained by electrospinning.
ta VH Abstract The incorporation of nanoparticles inside polymeric matrices has led to the development of multifunctional composites necessary to repair human tissues. The addition of nanoparticles may improve the properties of the composite materials such as surface area, mechanical properties, flexibility, hydrophilicity, electrical conductivity, etc. These properties can help in cellular growth, proliferation and/or differentiation. In this work, scaffolds of polycaprolactone (PCL) and reduced graphite oxide (rGO) were built by electrospinning technique. The ratios of rGO/PCL employed were 0.25, 0.5, 0.75 and 1...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Correa E, Moncada ME, Gutiérrez OD, Vargas CA, Zapata VH Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Evaluating Ternary Metal Oxide (TMO) core-shell nanocomposites for the rapid determination of the anti-neoplastic drug Chlorambucil (Leukeran ™) by electrochemical approaches.
Evaluating Ternary Metal Oxide (TMO) core-shell nanocomposites for the rapid determination of the anti-neoplastic drug Chlorambucil (Leukeran™) by electrochemical approaches. Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109724 Authors: Sakthivel K, Muthumariappan A, Chen SM, Li YL, Chen TW, Ali MA Abstract A functional ternary nanocomposite (MnO2@NiFe2O4) with hierarchically grown flower-like core-shell assembly has been prepared through a simple two-step hydrothermal methodology. The crystallographic information of the sample was obtained via X-ray diffraction. The morphological details of sph...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Sakthivel K, Muthumariappan A, Chen SM, Li YL, Chen TW, Ali MA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Transition metal-doped cryogels as bioactive materials for wound healing applications.
Abstract Transition metals (TM) are essential microelements with various biological functions demanded in tissue regeneration applications. Little is known about therapeutic potential of TM within soft hydrogel biomaterials. The soluble divalent TM, such as Zn, Cu, Mn and Co, were stably incorporated into gelatin network during cryogelation. TM content in the resultant cryogels varied from 0.1 × 103 to 11.8 × 103 ppm, depending on the TM type and concentration in the reaction solution. Zn component was uniformly complexed with the gelatin scaffold according to elemental imaging, increasing th...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Luong D, Yergeshov AA, Zoughaib M, Sadykova FR, Gareev BI, Savina IN, Abdullin TI Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Physicochemical and structural features of heat treated silver-silica nanocomposite and their impact on biological properties.
wak A Abstract In the last few decades, many nanostructures with varying properties and possible applications have been developed. These materials have been intended to work in various environmental temperature conditions. In this context, the main challenge has been to comprehend the impact of synergic interaction between individual elements included in non-annealed materials in relation to systems subjected to temperature impact. Another problem has corresponded to the impact of thermal modification on organisms such as bacteria and human cells. Such problems can be solved by the fabrication of a nanocomposite w...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Dulski M, Peszke J, Włodarczyk J, Sułowicz S, Piotrowska-Seget Z, Dudek K, Podwórny J, Malarz K, Mrozek-Wilczkiewicz A, Zubko M, Nowak A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Extracellular matrix electrospun membranes for mimicking natural renal filtration barriers.
Abstract Kidney diseases are recognized as a major health problem, which affect 10% of the population. Because currently available therapies have many limitations, some tissue engineering strategies have been emerging as promising approaches in this field. In this work, porcine kidneys were decellularized to obtain decellularized kidney extracellular matrix (dKECM).1 Our results demonstrate a successful protocol of decellularization characterized by the removal of nucleic acid material and preservation of collagen and glycosaminoglycans. Blends of polycaprolactone (PCL)2 and dKECM were prepared by electrospinning ...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Sobreiro-Almeida R, Fonseca DR, Neves NM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Improved bioavailability of Azelnidipine gastro retentive tablets-optimization and in-vivo assessment.
Abstract Azelnidipine, dihydropyridine based calcium channel blocker has been used for treating ischemic heart disease and cardiac remodeling after myocardial infarction but it is having a low bioavailability due to its poor solubility. The present study is to investigate the formulation and evaluation of floating tablets of Azelnidipine for controlled release and to increase bioavailability by increasing the gastrointestinal transit time and mucoadhesion of drug. The gastro retentive tablets were prepared by direct compression method using different concentrations of combination of Polyoxyethylene oxide WSR 303 a...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Gaikwad SS, Avari JG Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Advanced biomaterials for repairing and reconstruction of mandibular defects.
Abstract Mandibles are the largest and strongest bone in the human face and are often severely compromised by mandibular defects, compromising the quality of life of patients. Mandibular defects may result from trauma, inflammatory disease and benign or malignant tumours. The reconstruction of mandibular defect has been a research hotspot in oral and maxillofacial surgery. Although the principles and techniques of mandibular reconstruction have made great progress in recent years, the development of biomedical materials is still facing technical bottleneck, and new materials directly affect technological breakthro...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Zhang Q, Wu W, Qian C, Xiao W, Zhu H, Guo J, Meng Z, Zhu J, Ge Z, Cui W Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Electrochemical determination of clonazepam drug based on glassy carbon electrode modified with Fe3O4/R-SH/Pd nanocomposite.
Abstract Herein, fabrication and electrochemical evaluation of a new sensing platform, which is sensitive to clonazepam (CLZP) drug, has been illustrated. By using Fourier transform infrared spectrometry (FT-IR), electron microscopy (EM) techniques and vibrating sample magnetometer (VSM), synthesized nanocomposite, consisting of Fe3O4, 3-aminopropyltriethoxysilane (APTES), cyanuric chloride (CC), 2-mercaptoethanol and Pd (Fe3O4/R-SH/Pd), was characterized. Then using the common drop-casting method, the new nanocomposite was cast on the surface of bear glassy carbon electrode. The influence of effective experimenta...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Lotfi S, Veisi H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Electrospun asymmetric membranes for wound dressing applications.
sta S Abstract To accomplish a rapid wound healing it is necessary to develop an asymmetric membrane with interconnected pores consisting of a top layer that prevents rapid dehydration of the wound and bacteria penetration and a sub-layer with high absorption capacity and bactericidal properties. Polycaprolactone (PCL)/polyvinyl acetate (PVAc) asymmetric membranes loaded with the bactericidal monoterpene carvacrol (CRV) were synthesized and characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Mechanical properties in dry and wet conditions and fluid handling behavior were also...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Aragón J, Costa C, Coelhoso I, Mendoza G, Aguiar-Ricardo A, Irusta S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Multipotency expression of human adipose stem cells in filament-like alginate and gelatin derivative hydrogel fabricated through visible light-initiated crosslinking.
Abstract Hydrogel fibers are structurally and biologically useful devices for differentiation of stem cells and fabrication of filament-like tissues. We established cell-laden degradable hydrogel fibers through visible light-initiated crosslinking to differentiate stem cells and fabricate filament-like tissue. Human adipose stem cell (hADSC)-laden fibers were fabricated by cross-linking phenolic-substituted alginate and gelatin (Alg-Ph and Gela-Ph respectively) in an aqueous solution containing cells. The crosslinking of phenolic moieties was mediated by ruthenium(II) tris-bipyridyl dication (Ru(II) bpy and sodium...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Khanmohammadi M, Nemati S, Ai J, Khademi F Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

"In vitro" behaviour of aptamer-functionalized polymeric nanocapsules loaded with 5-fluorouracil for targeted therapy.
"In vitro" behaviour of aptamer-functionalized polymeric nanocapsules loaded with 5-fluorouracil for targeted therapy. Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109828 Authors: Rață DM, Cadinoiu AN, Atanase LI, Bacaita SE, Mihalache C, Daraba OM, Gherghel D, Popa M Abstract New type of nanocapsules based on carboxymethyl chitosan functionalized with AS1411 aptamer and poly(N-vinylpyrrolidone-alt-itaconic anhydride) loaded with 5-Fluorouracil (5-FU) were developed, with the potential to improve the treatment of cancer. Functionalization of nanocapsules with AS1411 aptamer will enhance t...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Rață DM, Cadinoiu AN, Atanase LI, Bacaita SE, Mihalache C, Daraba OM, Gherghel D, Popa M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation of antimicrobial metallic nanoparticles with bioactive compounds.
Abstract Despite the all recent advancements in medicine, infectious diseases continue to be major causes of death worldwide. Developing nanomaterials as preventive and therapeutic agents against infectious diseases has been one of the research priorities in medicine. However, the application of metal nanoparticles as antimicrobial agents is hampered due to environmental and safety concerns. Using green chemistry, researchers can produce biocompatible nanoparticles that have fewer detrimental effects on human health and the environment. Although chemical compounds have been considered as traditional sources for pr...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Amini SM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Facile synthesis of novel areca flower like Cu2O nanowire on copper foil for a highly sensitive enzyme-free glucose sensor.
Abstract The areca flower like Cu2O nanowire on Cu foil (AFL Cu2O NW@Cu) was synthesized by electrochemical anodization and thermal treatment, a simple and low-cost method. Composition and morphology of the AFL Cu2O NW@Cu were characterized by XRD, Raman, and SEM respectively. The copper substrate as the current collector played a key function as a source for the formation of areca flower like Cu2O nanowire. The Cu2O nanowire was grown on Cu foil and could connect with the Cu substrate without using any binder. The AFL Cu2O NW@Cu was utilized as an electrochemical enzyme-free sensor for glucose detection. This AFL...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Dat PV, Viet NX Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity.
Abstract Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polyme...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: De-Paula MMM, Afewerki S, Viana BC, Webster TJ, Lobo AO, Marciano FR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Design of colloidal drug carriers of celecoxib for use in treatment of breast cancer and leukemia.
l;ven M Abstract Inflammation develops initiation and pathological process of cancer. Nanoscale drug carriers are required to be investigated for delivery of actives at cellular level for treatment of various cancer types. Solid lipid nanoparticles (CLX-SLN), nanostructured lipid carriers (CLX-NLC) and a nanoemulsion (CLX-NE) of celecoxib (CLX), a selective cyclooxygenase-2 inhibitor, were formulated for use in remedy of breast cancer and acute promyelocytic leukemia. The hot high pressure homogenization technique was employed to product formulations. Scanning electron micrographs were utilized for morphological c...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Üner M, Yener G, Ergüven M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

An injectable cationic hydrogel electrostatically interacted with BMP2 to enhance in vivo osteogenic differentiation of human turbinate mesenchymal stem cells.
Abstract We have designed and characterized an injectable, electrostatically bonded, in situ-forming hydrogel system consisting of a cationic polyelectrolyte [(methoxy)polyethylene glycol-b-(poly(ε-caprolactone)-ran-poly(L-lactic acid)] (MP) copolymer derivatized with an amine group (MP-NH2) and anionic BMP2. To the best of our knowledge, there have been hardly any studies that have investigated electrostatically bonded, in situ-forming hydrogel systems consisting of MP-NH2 and BMP2, with respect to how they promote in vivo osteogenic differentiation of human turbinate mesenchymal stem cells (hTMSCs). Inje...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Kim MG, Kang TW, Park JY, Park SH, Ji YB, Ju HJ, Kwon DY, Kim YS, Kim SW, Lee B, Choi HS, Lee HB, Kim JH, Lee BY, Min BH, Kim MS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A miniaturized device for biomembrane permeation analysis.
In this study, we fabricated miniaturized multichannel devices (MCDs) by 3D printing, to minimize the use of skin and drug samples. The MCDs were compared with conventional static diffusion cells and achieved comparable drug permeation profiles. The finite element method-based simulation revealed the efficient carry-off of permeated ingredients by the multichannel devices, and a critical role of distance between the buffer stream and skin sample in determining the flow velocity inside the chamber. The results support these devices as qualified alternatives to Franz cells for in vitro permeation studies using biomembranes, ...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Ding D, Pan J, Yeo SH, Wagholikar V, Lim SH, Wu C, Fuh JYH, Kang L Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synergistic effect of 2D material coated Pt nanoparticles with PEDOT polymer on electrode surface interface for a sensitive label free Helicobacter pylori CagA(Ag-Ab) immunosensing.
Abstract Helicobacter pylori (H. pylori) immunosensor based on platinum nanoparticles/poly(3,4-ethylenedioxythiophene)/reduced graphene oxide (Ptnano/PEDOT/red-GOx) modified gold electrode (Au-ET) was stepwise fabricated for the detection of cytotoxin-associated gene A antibody (CagA antibody). H. pylori is a microaerophillic and a Gram-negative bacteria that causes gastric ulcer leading eventually to adenocarcinoma (gastric cancer) in the later stage. H. pylori colonizes inner lining of human stomach. The developed diagnostic sensing interface would allow H. pylori (stomach infection) detection in early stage and...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Gupta S, Tiwari A, Jain U, Chauhan N Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synergistic effects of ultrasound and photodynamic therapy leading to biofilm eradication on polyurethane catheter surfaces modified with hypericin nanoformulations.
In this study polyurethane (PUR) surfaces were coated with hypericin nanoformulations, leading to 4.3 log10 reduction in bacterial growth in vitro. The effect could be enhanced by the application of ultrasound. The combination of PDT with ultrasound therapy led to a synergistic effect resulting in a 6.8 log10 reduction of viable counts. This minimal invasive method requires only an optical fibre inserted in the catheter lumen and an ultrasound device. Thus the implementation in daily clinical practice is very simple. PMID: 31349520 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Vögeling H, Plenagl N, Seitz BS, Duse L, Pinnapireddy SR, Dayyoub E, Jedelska J, Brüßler J, Bakowsky U Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.
Abstract Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds. In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method. The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property. All silk fillers showed different degrees of improvement on printability in terms of ink rheology and...
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Zhang J, Allardyce BJ, Rajkhowa R, Kalita S, Dilley RJ, Wang X, Liu X Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A new nanocomposite scaffold based on polyurethane and clay nanoplates for osteogenic differentiation of human mesenchymal stem cells in vitro.
In conclusion, our results revealed that these biocompatible nanocomposites porous scaffolds with proper cell adhesion and proliferation as well as effective osteogenic differentiation and which are able to provide a new and useful matrix for bone tissue engineering purposes. PMID: 31349533 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - July 29, 2019 Category: Physiology Authors: Norouz F, Halabian R, Salimi A, Ghollasi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

99mTc-bisphosphonate-coated magnetic nanoparticles as potential theranostic nanoagent.
ić S Abstract Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc-bisphosphonate-coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1-hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, siz...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Mirković M, Radović M, Stanković D, Milanović Z, Janković D, Matović M, Jeremić M, Antić B, Vranješ-Đurić S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Coatings for biodegradable magnesium-based supports for therapy of vascular disease: A general view.
Abstract Metal stents are used as base material for fabrication of medical devices to support and improve the quality of life of patients with cardiovascular diseases such as arterial stenoses. Permanently present implants may induce responses that resemble adverse wound healing that compromise tissue function. A similar process namely restenosis, frequently may occur after the use of this kind of implants. However, the use of non-permanent, resorbable stents are a promising option to avoid this problem. The advantage of these implants is that they can degraded upon vascular repair. The most common metal used for ...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Echeverry-Rendon M, Allain JP, Robledo SM, Echeverria F, Harmsen MC Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Mechanical characterisation of multi vs. uni-directional carbon fiber frameworks for dental implant applications.
Abstract OBJECTIVES: The aim of the present study was to investigate the mechanical characteristics of dental implant frameworks made of unidirectional carbon fiber composite (UF) and to compare them with those provided by multidirectional carbon fiber composite (IF). METHODS: 8 identical UF samples were used. The samples were initially evaluated by optical microscope and SEM then non-destructive and destructive mechanical tests were performed on 4 samples in order to evaluate dynamic, static elastic modulus, wettability and ultimate strength. The outcomes were compared with those of IF samples tested followi...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Pesce P, Lagazzo A, Barberis F, Repetto L, Pera F, Baldi D, Menini M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Angiogenesis-promoting effect of LIPUS on hADSCs and HUVECs cultured on collagen/hyaluronan scaffolds.
Abstract Angiogenesis refers to blood vessel formation through endothelial cell migration and proliferation. Angiogenesis is crucial and beneficial for wound healing and tissue regeneration. In the current study, we prepared porous collagen and collagen/hyaluronan (Col/HA) scaffolds composed of collagen (7 mg/mL) and hyaluronan (HA) (0.5 w%, 1 w%, and 1.5 w%) as culture vehicles for coculture of human adipose-derived stem cells (hADSCs) and human umbilical vein endothelial cells (HUVECs). These scaffolds were combined with low-intensity pulsed ultrasound (LIPUS) to investigate and evaluate angiogenesis in the co...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Kang PL, Huang HH, Chen T, Ju KC, Kuo SM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Directly bonding antimicrobial peptide mimics to steel and the real world applications of these materials.
Abstract Despite increased sterilisation and education campaigns, hospital acquired infections have not been eradicated. Bacterial colonisation of frequent touch surfaces is key in the transmission of infection. Most current technologies cannot provide a material which can rapidly kill bacteria. Here we report a novel surface technology, which uses synthetic mimetics of human defensin proteins on a surface. The surface shows excellent antibacterial efficacy against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus, Pseudomonas aeruginosa and Escherichia coli. Both microbiology laboratory tests and tr...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Riordan L, Smith EF, Mills S, Hudson J, Stapley S, Nikoi ND, Edmondson S, Blair J, Peacock AFA, Scurr D, Forster G, de Cogan F Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Phyco-linked vs chemogenic magnetite nanoparticles: Route selectivity in nano-synthesis, antibacterial and acute zooplanktonic responses.
In conclusion, these findings demonstrate the optimized phyco-fabrication of Fe3O4-MNPs as promising nontoxic approach in ecobiotechnology, the new insight about the potential adverse effects of chemosynthesized Fe3O4-MNPs to crustacean zoo-organisms after their possible entrance into the marine environments, and bio/chemo-route Fe3O4-MNPs as pivotal agent for nanoantimicrobials. PMID: 31147005 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Mashjoor S, Yousefzadi M, Zolgharnein H, Kamrani E, Alishahi M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineering bio-mimetic humanized neurological constructs using acellularized scaffolds of cryopreserved meningeal tissues.
Abstract Spinal cord injury (SCI) is one of the most precarious conditions which have been one of the major reasons for continuous increasing mortality rate of SCI patients. Currently, there is no effective treatment modality for SCI patients posing major threat to the scientific and medical community. The available strategies don't mimic with the natural processes of nervous tissues repair/regeneration and majority of the approaches may induce the additional fibrotic or immunological response at the injury site and are not readily available on demand. To overcome these hurdles, we have developed a ready to use bi...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Vishwakarma SK, Lakkireddy C, Bardia A, Paspala SAB, Khan AA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Targeting human brain cancer stem cells by curcumin-loaded nanoparticles grafted with anti-aldehyde dehydrogenase and sialic acid: Colocalization of ALDH and CD44.
Abstract The use of chemotherapy against brain tumors faces various limitations to achieving its therapeutic effect, due to both the inability of anticancer agents to cross the blood-brain barrier (BBB) and the formation of brain cancer stem cells (BCSCs). Without adequate exposure, these chemotherapeutic drugs cannot have an antiproliferative effect on the tumors. Here, we developed curcumin (CCM)-loaded chitosan-poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) modified with sialic acid (SA) to permeate the BBB and with anti-aldehyde dehydrogenase (anti-ALDH) to target BCSCs. An increased chitosan concent...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Kuo YC, Wang LJ, Rajesh R Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering.
Abstract Hydrogen sulfide (H2S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenous and exogenous H2S plays a prominent role as a bone anabolic agent, we recently developed a silk fibroin (SF) porous scaffold loaded with GYY4137 (GYY), an H2S donor, for applications in bone tissue engineering. Here, we assayed whether the combination of SF with H2S potentiates the osteoconductive properties of SF. Biocompatibility and osteoanabolic activity were assayed in vitro using human bone marrow mesenchymal stromal cells. Cell cultures were perform...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Gambari L, Amore E, Raggio R, Bonani W, Barone M, Lisignoli G, Grigolo B, Motta A, Grassi F Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synthesis and characterization of ZnO nanoflakes anchored carbon nanoplates for antioxidant and anticancer activity in MCF7 cell lines.
Abstract ZnO nanoparticles with flakes-like structures were synthesized by simple wet chemical route using triethanolamine as a mild base. The well distributed ZnO nanoflakes onto carbon nanoplates (ZnO/C) were prepared by wet impregnation method. The crystalline structure and purity of the synthesized samples was inspected using XRD. The shape, structural morphology and elemental composition analysis was studied using FESEM and EDS. The probable anticancer activity of the synthesized samples was studied through their activity on human breast cancer MCF7 cell line. Exposure of breast cancer cells to ZnO and ZnO/C ...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Arasu MV, Madankumar A, Theerthagiri J, Salla S, Prabu S, Kim HS, Al-Dhabi NA, Arokiyaraj S, Duraipandiyan V Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Polyethylenimine modified with 2,3-dimethylmaleic anhydride potentiates the antitumor efficacy of conventional chemotherapy.
Abstract Conventional chemotherapy is a standard care for many cancers at present. However, their severe dose-dependent side effects are the major impediment for successful cancer therapy. Herein nanoparticles were used as a potentiator to enhance the uptake of free chemotherapeutic agents by cancer cells during chemotherapy. A pH-sensitive β-carboxylate amide group-containing polymer, bPEI-DMA, was obtained by a one-step chemical reaction of commercially available branched polyethyleneimine with 2,3-dimethylmaleic anhydride. The obtained single-macromolecule nanoparticles with a size of 6.4 nm possessed zw...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Huang X, Cao J, Zhang Y, Liu T, Yan H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Sinusoidal voltage electrodeposition of PEDOT-Prussian blue nanoparticles composite and its application to amperometric sensing of H2O2 in human blood.
Abstract A selective electrochemical sensor based on poly(3,4-ethylenedioxythiophene) (PEDOT) - Prussian blue nanoparticles (PBNPs) for hydrogen peroxide (H2O2) determination was prepared by innovative sinusoidal voltage (SV) method. The successful incorporation of citrate-stabilized PBNPs into PEDOT matrix was confirmed by energy dispersive X-ray analysis (EDX), Raman spectroscopy, UV-Vis spectroelectrochemistry and cyclic voltammetry measurements. The SV preparation method provides a PEDOT-PBNPs coating with rough surface morphology and good electrocatalytic activity toward H2O2 reduction. The amperometric respo...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Lete C, Marin M, Anghel EM, Preda L, Matei C, Lupu S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Evaluating two ovarian decellularization methods in three species.
Abstract Since there is dearth of practical ways to obtain mature follicles from cryopreserved or native ovarian tissues, especially in patients suffering from ovarian dysfunction, tissue engineering may help in restoring ovarian function and/or fertility. In the present study, the effects of sodium dodecyl sulfate (SDS) and sodium hydroxide (NaOH) on the decellularization of ovarian tissues were studied in order to ascertain their suitability in creating suitable bioscaffolds. Cells were removed from the ovarian tissues of mouse, sheep and human. The samples were distributed among three groups, viz., control (not...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Eivazkhani F, Abtahi NS, Tavana S, Mirzaeian L, Abedi F, Ebrahimi B, Montazeri L, Valojerdi MR, Fathi R Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Efficient nanoparticles removal and bactericidal action of electrospun nanofibers membranes for air filtration.
any M Abstract Human exposure to air pollution and especially to nanoparticles is increasing due to the combustion of carbon-based energy vectors. Fibrous filters are among the various types of equipment potentially able to remove particles from the air. Nanofibers are highly effective in this area; however, their utilization is still a challenge due to the lack of studies taking into account both nanoparticle collection efficiency and antibacterial effect. The aim of this work is to produce and evaluate novel silver/polyacrylonitrile (Ag/PAN) electrospun fibers deposited on a nonwoven substrate to be used as air ...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Bortolassi ACC, Nagarajan S, de Araújo Lima B, Guerra VG, Aguiar ML, Huon V, Soussan L, Cornu D, Miele P, Bechelany M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineering microenvironments towards harnessing pro-angiogenic potential of mesenchymal stem cells.
In this study, human bone marrow-derived MSCs were seeded on matrices with varying stiffness or cell adhesive sites, and conditioned media was collected. The concentrations of angiogenic molecules in the media was measured via ELISA. In addition, the bioactivity of the released molecules was investigated via assessing the proliferation and capillary morphogenesis of human umbilical vein endothelial cells (HUVECs) incubated with conditioned media. Our study revealed that secretion of vascular endothelial growth factor (VEGF) is dependent on substrate stiffness. Maximal secretion was observed when MSCs were seeded on hydroge...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Nasser M, Wu Y, Danaoui Y, Ghosh G Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Chondrogenesis of human mesenchymal stem cells by microRNA loaded triple polysaccharide nanoparticle system.
In this study chondroitin sulfate/hyaluronic acid/chitosan nanoparticles were prepared and successfully characterized chemically and morphologically. Results demonstrated higher chondroitin sulfate amounts led smaller nanoparticles, but lower surface zeta potential due to high electronegativity. After optimization of chondroitin sulfate amounts regarding size and charge, nanoparticles were loaded with microRNA-149-5p, a therapeutic miRNA downregulated in osteoarthritis, and evaluated focusing on their loading efficiency, release behaviour, cytotoxicity and gene transfection efficiency in vitro. Results showed all nanoparti...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Çelik E, Bayram C, Denkbaş EB Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Silver-nanoparticles as plasmon-resonant enhancers for eumelanin's photoacoustic signal in a self-structured hybrid nanoprobe.
This study addresses both issues through the design of a biocompatible photoacoustic nanoprobe, named MelaSil_Ag-NPs, relying on silica-templated eumelanin formation as well as eumelanins redox and metal chelating properties to reduce Ag+ ions and control the growth of generated metal nanoparticles. This strategy allowed self-structuring of the system into a core-shell architecture, where the Ag core was found to boost PA signal, despite the poor eumelanin content. Obtained hybrid nanoplatforms, showed stable photoacoustic properties even under long irradiation. Furthermore, conjugation with rhodamine isothiocyanate allowe...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Silvestri B, Armanetti P, Sanità G, Vitiello G, Lamberti A, Calì G, Pezzella A, Luciani G, Menichetti L, Luin S, d'Ischia M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Graphene/nickel oxide nanocomposites against isolated ESBL producing bacteria and A549 cancer cells.
Abstract The synthesis of nickel oxide nanoparticles (NiO NPs) and graphene/nickel oxide nanocomposites (Gr/NiO NCs) was performed using a simple chemical reduction method. Powder X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were used to examine the crystalline nature and thermal stability of the synthesized NiO NPs and Gr/NiO NCs, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were utilized to observe the morphology of NiO NPs and Gr/NiO NCs and estimate their size range. TEM suggested that the NiO NPs were speared onto the surface of Gr nanosheet. The ...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Rajivgandhi G, Maruthupandy M, Quero F, Li WJ Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biodegradable nanoparticles exposing a short anti-FLT1 peptide as antiangiogenic platform to complement docetaxel anticancer activity.
Abstract Inhibition of tumor angiogenesis is considered as a valuable clinical strategy to treat some tumors, although benefits in term of progression-free and overall survival have been modest. Recent findings have pushed toward the use of antiangiogenic drugs in combination with chemotherapy regimens to potentiate therapeutic outcome. Herein, we propose a novel type of biodegradable antiangiogenic core-shell polymeric nanoparticles (NPs) for the delivery of poorly water-soluble chemotherapeutics. An amphiphilic diblock copolymer of poly(ethyleneglycol)-poly(ε-caprolactone) (PEG-PCL) was conjugated with a...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Conte C, Moret F, Esposito D, Dal Poggetto G, Avitabile C, Ungaro F, Romanelli A, Laurienzo P, Reddi E, Quaglia F Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Co-culture of human umbilical vein endothelial cells and human bone marrow stromal cells into a micro-cavitary gelatin-methacrylate hydrogel system to enhance angiogenesis.
In this study, a bioinspired 3D gelatin-methacrylate (Gel-MA) hydrogel with dissolvable microspheres was created to encapsulate human bone marrow stromal cells (HMSCs) and human umbilical vein endothelial cells (HUVECs) which was used to investigate whether HMSCs could play a pericytes-like role and enhance vascularization within the engineered scaffolds. The results showed co-culture of HMSCs and HUVECs demonstrated significantly improved vascularization when compared to either HUVECs or HMSCs monoculture. Angiogenic genes were expressed significantly higher in co-culture group. Moreover, when implanting the pre-vasculari...
Source: Appl Human Sci - May 31, 2019 Category: Physiology Authors: Liu J, Chuah YJ, Fu J, Zhu W, Wang DA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fast degrading elastomer stented fascia remodels into tough and vascularized construct for tracheal regeneration.
In this study, we developed a three-layer, elastomeric, trachea-like composite made of poly glycerol sebacate (PGS) and polycaprolactone (PCL), which presented appropriate resilient property, timely degradation and interconnected pores. C shape PCL rings fabricated with selective laser sintering (SLS) techniques are regularly positioned around porous PGS tubes and fixed by PCL electrospinning sheath. Such an elastomeric composite underwent host remodeling including rapid vascularization and tissue infiltration after fascia wrapping. With degrading of PGS, C rings well incorporated into growing fascia and lead to the format...
Source: Appl Human Sci - April 30, 2019 Category: Physiology Authors: Wu W, Jia S, Chen W, Liu X, Zhang S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research