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Evaluating adhesion and alignment of dental pulp stem cells to a spider silk substrate for tissue engineering applications.
This study evaluated the response of human DPSCs to spider dragline silk fibers, a potential substrate material for tissue regeneration. The DPSCs' morphology and spread pattern were characterized after these cells were plated onto Nephila clavipes dragline fibers in media. In addition, the responses of two other well established cell lines, osteoblasts (7F2s), and fibroblasts (3T3s), were also studied under identical conditions. The inclusion of 3T3s and 7F2s in this study allowed for both direct comparisons to prior published work and a qualitative comparison to the morphology of the DPSCs. After twelve days, the DPSCs e...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Hafner K, Montag D, Maeser H, Peng C, Marcotte WR, Dean D, Kennedy MS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Engineered electrospun polycaprolactone (PCL)/octacalcium phosphate (OCP) scaffold for bone tissue engineering.
Abstract The main challenge in bone tissue engineering is to find suitable biological substitutes which act as scaffolds. Hence, in this work, a novel scaffold composed of octacalcium phosphate (OCP) particles fabricated by co-precipitation method and polycaprolactone (PCL) using electrospinning technique was introduced. The electrospun scaffolds were characterized by SEM, FTIR, XRD, DSC and TGA analysis. The mechanical properties of the composite scaffolds including maximum tensile stress, strain at break and Young modulus were measured. The bioactivity of the scaffolds was determined by soaking in simulated body...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Heydari Z, Mohebbi-Kalhori D, Afarani MS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Facile-one pot-green synthesis, antibacterial, antifungal, antioxidant and antiplatelet activities of lignin capped silver nanoparticles: A promising therapeutic agent.
Abstract The current work portrays the green synthesis of Lignin Capped Silver Nanoparticles (LCSN) and their antibacterial, antifungal, antioxidant and antiplatelet potential. The LCSN was synthesized in water using a carbohydrate based polymer 'lignin' as the reducing and capping agents. The peak at 406nm (λmax) in the UV-Vis., spectrum and EDX analysis confirmed 1.68% (w/w) of silver was found to be loaded on lignin. The characteristic sharp peaks appeared in the PXRD spectrum showed fcc crystalline structure LCSN. SEM and TEM images indicated that the spherical Ag-NPs were well dispersed on lignin with ...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Marulasiddeshwara MB, Dakshayani SS, Sharath Kumar MN, Chethana R, Raghavendra Kumar P, Devaraja S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber.
Abstract Ulcerative colitis (UC) is a main form of inflammatory bowel disease (IBD). Asafoetida (ASF) and turmeric have traditionally been used for the treatment of various inflammatory diseases, including UC, because ASF is rich in sulfur compounds and turmeric contains curcumin (CUR). Turmeric nanofiber (TNF), the modified cell wall component of turmeric is considered to play important role in the human diet, health and can be used as a carrier agent to encapsulate bioactive components. A novel gut health product (GHP) was formulated by encapsulation of ASF and CUR complex onto TNF. The GHP was characterized by ...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Gopi S, Amalraj A, Jude S, Varma K, Sreeraj TR, Haponiuk JT, Thomas S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Viability of HEK 293 cells on poly- β-hydroxybutyrate (PHB) biosynthesized from a mutant Azotobacter vinelandii strain. Cast film and electrospun scaffolds.
Viability of HEK 293 cells on poly-β-hydroxybutyrate (PHB) biosynthesized from a mutant Azotobacter vinelandii strain. Cast film and electrospun scaffolds. Mater Sci Eng C Mater Biol Appl. 2017 Dec 01;81:236-246 Authors: Romo-Uribe A, Meneses-Acosta A, Domínguez-Díaz M Abstract Sterilization, cytotoxicity and cell viability are essential properties defining a material for medical applications and these characteristics were investigated for poly(β-hydroxybutyrate) (PHB) of 230kDa obtained by bacterial synthesis from a mutant strain of Azotobacter vinelandii. Cell viability was i...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Romo-Uribe A, Meneses-Acosta A, Domínguez-Díaz M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Nanoclay cross-linked semi-IPN silk sericin/poly(NIPAm/LMSH) nanocomposite hydrogel: An outstanding antibacterial wound dressing.
Abstract High antibacterial and skin-like hydrogels have always been the perfect wound dressing for human to protect wound from infection. Here, based on silk sericin, we design a series of nanoclay lithium magnesium silicate hydrate (LMSH) cross-linked semi-IPN sericin/poly(NIPAm/LMSH) (HSP) nanocomposite hydrogels and demonstrate advantages in serving as antibacterial wound dressing in comparison with gauze. Firstly, the effect of mass ratios of sericin/(sericin+NIPAm) upon pore structure, feasibility of mechanics and gas permeability of HSP nanocomposite hydrogels were evaluated. Then, the relationship between ...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Yang C, Xue R, Zhang Q, Yang S, Liu P, Chen L, Wang K, Zhang X, Wei Y Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

The negative effect of silica nanoparticles on adipogenic differentiation of human mesenchymal stem cells.
The objective of this study is to investigate the in vitro uptake of silica nanoparticles (silica NPs) and their effect on adipogenic differentiation of hMSCs. After exposure of hMSCs to silica NPs, the uptake and localization of silica NPs were assessed using transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The adipogenic differentiation potential of hMSCs was examined by analyzing the formation and accumulation of lipids droplets, triglyceride (TG) content and the expression of adipogenic marker genes/proteins. The results showed that silica NPs did not affect the cell viability but s...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Yang X, Liu X, Li Y, Huang Q, He W, Zhang R, Feng Q, Benayahu D Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

PEGylated stereocomplex polylactide coating of stent for upregulated biocompatibility and drug storage.
Abstract Treatment of coronary heart disease by percutaneous coronary intervention (PCT) is usually limited to the high restenosis rate after implantation of bare-metal stent. To solve the problem, the coating of PEGylated stereocomplex poly(l-lactide) (PEG-cPLA) was utilized on the surface modification of stainless steel (SS) sheet. Specifically, the 3-aminopropyltriethoxysilane (APTES)-modified methoxy-poly(ethylene glycol)-poly(d-lactide) (mPEG-PDLA) was grafted onto the surface of hydroxylated SS sheet through coupling reaction, and poly(l-lactide)-poly(ethylene glycol)-poly(l-lactide) (PLLA-PEG-PLLA) was coat...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Li Z, Jiang Z, Zhao L, Yang X, Zhang J, Song X, Liu B, Ding J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A targeted nanocarrier based on polyspermine for the effective delivery of methotrexate in nasopharyngeal carcinoma.
Abstract Antitumor efficacy of methotrexate (MTX) is seriously limited due to its poor water solubility, nonspecific tumor distribution and low bioavailability. To overcome these obstacles, polyspermine (PSP) conjugated with MTX and folic acid (FA) as a novel targeted prodrug was designed and has been successfully synthesized using the amidation reaction. The strong hydrophilic properties of PSP made MTX well dispersed in water and the cellular uptake study indicated that the presence of FA enhanced uptake of the FA-PSP-MTX in folate receptor (FR) over-expressing human nasopharyngeal carcinoma HNE-1 cells. (1)H NM...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Ji X, Guo H, Tang Q, Ma D, Xue W Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Synthesis and evaluation of the cytotoxic and anti-proliferative properties of ZnO quantum dots against MCF-7 and MDA-MB-231 human breast cancer cells.
Abstract Current trends in therapeutic research are the application of nanomaterial carriers for cancer therapy. One such molecule, ZnO, originally used in diagnosis and as a drug carrier, is gaining importance for its biological properties. Here, we report for the first time, the scope of ZnO QDs for enhanced cytotoxicity against MCF-7 and metastatic MDA-MB-231 human breast cancer cells. Unlike other ZnO nanostructures, ZnO QDs are dispersed and small sized (8-10nm) which is believed to greatly increase the cellular uptake. Furthermore, the acidic tumor microenvironment attracts ZnO QDs enhancing targeted therapy...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: A R, Jagadeesan S, Cho YJ, Lim JH, Choi KH Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Functionalization of titanium dioxide nanotubes with biomolecules for biomedical applications.
The objective of this review is to show the main techniques of immobilization of biomolecules in TNTs, evidencing the most recent applications of bioactive molecules that have been functionalized in the nanotubular matrices for use in implants and biosensors. This surveillance also proposes a new class of biomolecules that can be used to functionalize these nanostructured surfaces, lectins. PMID: 28888015 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Oliveira WF, Arruda IRS, Silva GMM, Machado G, Coelho LCBB, Correia MTS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Albumin based versatile multifunctional nanocarriers for cancer therapy: Fabrication, surface modification, multimodal therapeutics and imaging approaches.
Abstract Albumin is a versatile protein used as a carrier system for cancer therapeutics. As a carrier it can provide tumor specificity, reduce drug related toxicity, maintain therapeutic concentration of the active moiety like drug, gene, peptide, protein etc. for long period of time and also reduce drug related toxicities. Apart from cancer therapy, it is also utilized in the imaging and multimodal therapy of cancer. This review highlights the important properties, structure and types of albumin based nanocarriers with regards to their use for cancer targeting. It also provides brief discussion on methods of pre...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Kudarha RR, Sawant KK Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

In-vivo characterization of a 3D hybrid scaffold based on PCL/decellularized aorta for tracheal tissue engineering.
CONCLUSION: Harvested structures were totally viable due to the angiogenesis created by the muscle. H&E and alcian blue staining results revealed the presence of chondrocytes in the structure and GAG in the produced extracellular matrix. Since tracheal replacement using biologic and synthetic scaffolds usually results in tracheal collapse or granulation formation, a hybrid construct may provide the required rigidity and biocompatibility for the substitute. PMID: 28888019 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Ghorbani F, Moradi L, Shadmehr MB, Bonakdar S, Droodinia A, Safshekan F Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field.
Abstract With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of ~40nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in...
Source: Appl Human Sci - September 11, 2017 Category: Physiology Authors: Matsuda S, Nakajima E, Nakanishi T, Hitsuji A, Zhang H, Tanaka A, Matsuda H, Momma T, Osaka T Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Glycerol-plasticised silk membranes made using formic acid are ductile, transparent and degradation-resistant.
This study investigated the use of formic acid as the solvent with glycerol to make deformable yet degradation-resistant silk membranes. Here we show that membranes cast using formic acid had low light scattering, with a diffuse transmittance of less than 5% over the visible wavelengths, significantly lower than the 20% transmittance of aqueous derived silk/glycerol membranes. They had 64% β-sheet content and lost just 30% of the initial silk weight over 6h when tested with an accelerated enzymatic degradation assay, in comparison the aqueous membranes completely degraded within this timeframe. The addition of glycero...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Allardyce BJ, Rajkhowa R, Dilley RJ, Redmond SL, Atlas MD, Wang X Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A novel sensor made of Antimony Doped Tin Oxide-silica composite sol on a glassy carbon electrode modified by single-walled carbon nanotubes for detection of norepinephrine.
In this study, we designed a novel molecularly imprinted polymer (MIP), Antimony Doped Tin Oxide (ATO)-silica composite sol, which was made using a sol-gel method. Then a sensitive and selective imprinted electrochemical sensor was constructed with the ATO-silica composite sol on a glassy carbon electrode modified by single-walled carbon nanotubes (SWNTs). The introduction of SWNTs increased the sensitivity of the MIP sensor. The surface morphology of the MIP and MIP/SWNTs were characterized by scanning electron microscopy (SEM), and the optimal conditions for detection were determined. The oxidative peak current increased...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Wang Z, Wang K, Zhao L, Chai S, Zhang J, Zhang X, Zou Q Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Use of a fluorescent probe to monitor the enhanced affinity of rh-BMP-2 to silicated-calcium phosphate synthetic bone graft substitutes under competitive conditions.
Abstract A comparative investigation was undertaken on 1-2mm sized granules of two forms of synthetic bone graft substitute (SBG) with identical pore structure but varied bulk chemistry, stoichiometric hydroxyapatite (HA) and silicate substituted (0.8wt% Si) hydroxyapatite (SA), to assess the influence of SBG chemistry on the relative affinity of an osteogenic growth factor (GF), recombinant human bone morphogenetic protein-2 (rhBMP-2). A previously described novel fluorescent probe, fluoresceinthioureidoaminocaproic acid (FTCA), was covalently attached to rhBMP-2 to give FTCA-rhBMP-2 and facilitate the quantitati...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Mafina MK, Sullivan AC, Hing KA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biomimetic modification of polyurethane-based nanofibrous vascular grafts: A promising approach towards stable endothelial lining.
Abstract The emerging demand for small caliber vascular grafts to replace damaged vessels has attracted research attention. However, there is no perfect replacement in clinical use yet, mainly due to low patency rate of synthetic small caliber grafts. The main pathology behind low patency rate include thrombosis and graft/vessel hemodynamic mismatch, leading to intimal hyperplasia. Rapid in-situ endothelialization of vascular grafts is considered as one of the best strategies to overcome these complications. In the present study, Heparin and VEGF were immobilized via self-polymerization and deposition of polydopam...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Davoudi P, Assadpour S, Derakhshan MA, Ai J, Solouk A, Ghanbari H Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Influence of bovine serum albumin in Hanks' solution on the corrosion and stress corrosion cracking of a magnesium alloy.
Abstract It is essential for any temporary implant to possess adequate strength to maintain their mechanical integrity under the synergistic effects of mechanical loading characteristics of human body and the corrosive physiological environment. Such synergistic effects can cause stress corrosion cracking (SCC). The aim of the present study is to investigate the effect of the addition of bovine serum albumin (BSA) to Hanks' solution in corrosion and SCC susceptibility of AZ91D magnesium alloy. The electrochemical impedance spectroscopy (EIS) results indicated that the addition of BSA increased corrosion resistance...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Harandi SE, Banerjee PC, Easton CD, Singh Raman RK Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A strategy to promote the electroactive platform adopting poly(o-anisidine)-silver nanocomposites probed for the voltammetric detection of NADH and dopamine.
Abstract A study on the voltammetric detection of NADH (β-nicotinamide adenine dinucleotide), Dopamine (DA) and their simultaneous determination is presented in this work. The electrochemical sensor was fabricated with the hybrid nanocomposites of poly(o-anisidine) and silver nanoparticles prepared by simple and cost-effective insitu chemical oxidative polymerization technique. The nanocomposites were synthesized with different (w/w) ratios of o-anisidine and silver by increasing the amount of o-anisidine in each, by keeping silver at a fixed quantity. The XRD patterns revealed the semi-crystalline nature of ...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Sangamithirai D, Munusamy S, Narayanan V, Stephen A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Monitoring structural features, biocompatibility and biological efficacy of gamma-irradiated methotrexate-loaded spray-dried microparticles.
This study demonstrated the feasibility of the gamma irradiated spray dried PLGA microparticles for prolonged release of MTX, supporting a promising antitumor-drug delivery system for parenteral (subcutaneous) or pulmonary use. PMID: 28866185 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: de Oliveira AR, Mesquita PC, Machado PRL, Farias KJS, de Almeida YMB, Fernandes-Pedrosa MF, Cornélio AM, do Egito EST, da Silva-Júnior AA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

PLLA scaffolds produced by thermally induced phase separation (TIPS) allow human chondrocyte growth and extracellular matrix formation dependent on pore size.
zil G Abstract Damage of hyaline cartilage species such as nasoseptal or joint cartilage requires proper reconstruction, which remains challenging due to the low intrinsic repair capacity of this tissue. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. The aim of this work was to assess the viability, attachment, morphology, extracellular matrix (ECM) production of human articular and nasoseptal chondrocytes cultured in vitro in porous poly(l-lactic) (PLLA) scaffolds of two selected pore sizes (100 and 200μm). The P...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Conoscenti G, Schneider T, Stoelzel K, Carfì Pavia F, Brucato V, Goegele C, La Carrubba V, Schulze-Tanzil G Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Nanosized CaP-silk fibroin-PCL-PEG-PCL/PCL based bilayer membranes for guided bone regeneration.
In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA)(1) and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content. The structural and chemical analyses revealed the well-integrated two layers of membranes with a total thickness of ca 100μm. In the regenerative layer, the highly porous mesh structure had a ...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Türkkan S, Pazarçeviren AE, Keskin D, Machin NE, Duygulu Ö, Tezcaner A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Fabrication and characterization of hydrothermal cross-linked chitosan porous scaffolds for cartilage tissue engineering applications.
Abstract The use of various chemical cross-linking agents for the improvement of scaffolds physical and mechanical properties is a common practical method, which is limited by cytotoxicity effects. Due to exerting contract type forces, chondrocytes are known to implement shrinkage on the tissue engineered constructs, which can be avoided by the scaffold cross-linking. In the this research, chitosan scaffolds are cross-linked with hydrothermal treatment with autoclave sterilization time of 0, 10, 20 and 30min, to avoid the application of the traditional chemical toxic materials. The optimization studies with gel co...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Shamekhi MA, Rabiee A, Mirzadeh H, Mahdavi H, Mohebbi-Kalhori D, Baghaban Eslaminejad M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biocompatible curcumin loaded PMMA-PEG/ZnO nanocomposite induce apoptosis and cytotoxicity in human gastric cancer cells.
Abstract Although curcumin is efficient in killing cancer cells, its poor water solubility and assocaited inadequate bioavailability remain major limitations to its therapeutic application. The formulation of curcumin micellar nanoparticles (NPs) encapsulated with a biodegradable polymer promises to significantly improve curcumin's solubility, stability, and bioavailability. The past decade has witnessed the development of nanoscale curcumin delivery systems: curcumin-loaded liposomes or nanoparticles, self-microemulsifying drug delivery systems (SMEDDS), cyclodextrin inclusions, solid dispersions, nanodisks, and ...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Dhivya R, Ranjani J, Bowen PK, Rajendhran J, Mayandi J, Annaraj J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

ZnO@Gd2O3 core/shell nanoparticles for biomedical applications: Physicochemical, in vitro and in vivo characterization.
Abstract The chemical composition of nanoparticles (NPs) may be so designed as to provide measurability for numerous imaging techniques in order to achieve synergistic advantages. Innovative and unique structure of the core/shell ZnO@Gd2O3 NPs possesses luminescent and magnetic properties, and is expected that they will become a new generation of contrast agents for Magnetic Resonance Imaging (MRI) and nanocarriers for theranostics. Thus, by surface biofunctionalization, it is possible to indicate particular nanoparticle compositions which provide efficient imaging, targeted drug delivery, and biocompatibility. No...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Woźniak A, Grześkowiak BF, Babayevska N, Zalewski T, Drobna M, Woźniak-Budych M, Wiweger M, Słomski R, Jurga S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

The application of silver nano-particles on developing potential treatment for chronic rhinosinusitis: Antibacterial action and cytotoxicity effect on human nasal epithelial cell model.
Abstract Chronic rhinosinusitis (CRS) has raised attentions both in many countries due to its high relapsing rate and the resistance of certain treatments especially antibiotics application on both acute and chronic bacterial rhinosinusitis. The aim of this research is stressing on developing an effective alternative treatment for treating CRS and reducing the use of antibiotics to avoid further resistance forming. The antibacterial functions of silver nano-particles (AgNPs) are well known according to previous reports and studies. However, for developing a suitable treatment for further clinical application, a va...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Chen SY, Huang MT, Pender SLF, Ruslin M, Chou HH, Ou KL Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Growth of a TiNb adhesion interlayer for bioactive coatings.
te;ková L, Špatenka P Abstract Surface bioactivity has been under intensive study with reference to its use in medical implants. Our study is focused on coatings prepared from an electroactive material which can support bone cell adhesion. Until now, hydroxyapatite films have usually been utilized as a chemically-active surface agent. However, electrically-active films could set a new direction in hard tissue replacement. As a base for these films, it is necessary to prepare an intermediate film, which can serve as a suitable barrier against the possible diffusion of some allergens and toxic elements...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Tolde Z, Starý V, Cvrček L, Vandrovcová M, Remsa J, Daniš S, Krčil J, Bačáková L, Špatenka P Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Multifunctional biosynthesized silver nanoparticles exhibiting excellent antimicrobial potential against multi-drug resistant microbes along with remarkable anticancerous properties.
This study demonstrates the therapeutic potential of silver nanoparticles (AgNPs), which were biosynthesized using the extracts of Citrus maxima plant. Characterization through UV-Vis spectrophotometry, Dynamic Light Scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) confirmed the formation of AgNps in nano-size range. These nanoparticles exhibited enhanced antioxidative activity and showed commendable antimicrobial activity against wide range of microbes including multi-drug resistant bacteria that were later confirmed by TEM. These particle...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Jha D, Thiruveedula PK, Pathak R, Kumar B, Gautam HK, Agnihotri S, Sharma AK, Kumar P Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Detection of alprazolam with a lab on paper economical device integrated with urchin like Ag@ Pd shell nano-hybrids.
We present results of the studies relating to fabrication of a microfluidic biosensor chip based on urchin like Ag@ Pd shell nano-hybrids that is capable of sensing alprazolam through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of alprazolam present in buffer solutions at clinically relevant concentrations. Methylene blue (MB) was also doped as redox transition substance for sensing alprazolam. Nano-hybrids modified EμPAD showed wide linear range 1-300ng/ml and low detection limit of 0.025ng/l. Low detection limit can further enhance its ...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Narang J, Malhotra N, Singhal C, Mathur A, Pn AK, Pundir CS Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Highlights in nanocarriers for the treatment against cervical cancer.
da AM Abstract Cervical cancer is the second most common malignant tumor in women worldwide and has a high mortality rate, especially when it is associated with human papillomavirus (HPV). In US, an estimated 12,820 cases of invasive cervical cancer and an estimated 4210 deaths from this cancer will occur in 2017. With rare and very aggressive conventional treatments, one sees in the real need of new alternatives of therapy as the delivery of chemotherapeutic agents by nanocarriers using nanotechnology. This review covers different drug delivery systems applied in the treatment of cervical cancer, such as solid li...
Source: Appl Human Sci - September 4, 2017 Category: Physiology Authors: Medina-Alarcón KP, Voltan AR, Fonseca-Santos B, Moro IJ, de Oliveira Souza F, Chorilli M, Soares CP, Dos Santos AG, Mendes-Giannini MJS, Fusco-Almeida AM Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

A novel low-cost solution for driving assessment in individuals with and without disabilities.
Abstract Brake reaction time is a key component to studying driving performance and evaluating fitness to drive. Although commercial simulators can measure brake reaction time, their cost remains a major barrier to clinical access. Therefore, we developed open-source software written in C-sharp (C#) for measuring driving related reaction times, which includes a subject-controlled vehicle with straight-line dynamics and several testing scenarios. The software measures both simple and cognitive load based reaction times and can use any human interface device compliant steering wheel and pedals. Measures from the sof...
Source: Appl Human Sci - August 15, 2017 Category: Physiology Authors: Rodseth J, Washabaugh EP, Al Haddad A, Kartje P, Tate DG, Krishnan C Tags: Appl Ergon Source Type: research

Formulation and in-vitro efficacy of antifungal mucoadhesive polymeric matrices for the delivery of miconazole nitrate.
rdi D Abstract Oral candidiasis is the most common opportunistic infection affecting patients with the human immunodeficiency virus. Miconazole buccal tablets or miconazole gel are approved for the treatment of oropharyngeal candidiasis. However, buccal films present more flexibility and also offer protection for the wounded mucosa, reducing pain. Due to their small size and thickness, buccal films may improve patients' compliance, compared to tablets. Additionally, they may increase the relatively short residence time on the mucosa of oral gels, which are easily removed by saliva. Polymeric films loaded with mico...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Tejada G, Piccirilli GN, Sortino M, Salomón CJ, Lamas MC, Leonardi D Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Biomimetic electrospun scaffolds from main extracellular matrix components for skin tissue engineering application - The role of chondroitin sulfate and sulfated hyaluronan.
Abstract Incorporation of bioactive components like glycosaminoglycans (GAGs) into tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion. The effects of these scaffolds were ...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Bhowmick S, Rother S, Zimmermann H, Lee PS, Moeller S, Schnabelrauch M, Koul V, Jordan R, Hintze V, Scharnweber D Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

The Bone Building Blues: Self-hardening copper-doped calcium phosphate cement and its in vitro assessment against mammalian cells and bacteria.
Abstract A blue calcium phosphate cement with optimal self-hardening properties was synthesized by doping whitlockite (β-TCP) with copper ions. The mechanism and the kinetics of the cement solidification process were studied using energy dispersive X-ray diffraction and it was found out that hardening was accompanied by the phase transition from TCP to brushite. Reduced lattice parameters in all crystallographic directions resulting from the rather low (1:180) substitution rate of copper for calcium was consistent with the higher ionic radius of the latter. The lower cationic hydration resulting from the part...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Rau JV, Wu VM, Graziani V, Fadeeva IV, Fomin AS, Fosca M, Uskoković V Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Methotrexate-loaded nitrogen-doped graphene quantum dots nanocarriers as an efficient anticancer drug delivery system.
In this study, blue fluorescent nitrogen-doped GQDs (N-GQDs) were synthesized by a hydrothermal method via pyrolisis of citric acid as the carbon source and urea as the nitrogen source. The existence of doped nitrogen in GQDs was confirmed by FTIR characterization. Here, for the first time, the N-GQDs were loaded with the anticancer drug, methotrexate (MTX), to prepare MTX-(N-GQDs) as an efficient drug delivery system. The establishment of the strong π-π stacking interaction between MTX and N-GQDs was confirmed by FTIR and UV-vis spectroscopies indicating successful loading of MTX to N-GQDs. The in-vitro cytotoxicity...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Khodadadei F, Safarian S, Ghanbari N Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Novel hiPSC-based tri-culture for pre-vascularization of calcium phosphate scaffold to enhance bone and vessel formation.
In this study, a novel tri-culture system was developed to elicit pre-vascularization of calcium phosphate cement (CPC) scaffold in which human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSMSCs) were seeded together with human umbilical vein endothelial cells (HUVECs) and pericytes. In a two-step methodology design, we first performed osteoinduction of the seeded hiPSMSCs on the CPC scaffold and then incorporated HUVECs and pericytes to the hiPSMSC-colonized CPC scaffold under a favorable culturing condition, with an objective to form a stable and functional capillary-like vascular network that sustain...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Zhang C, Hu K, Liu X, Reynolds MA, Bao C, Wang P, Zhao L, Xu HHK Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Two-phase electrospinning to incorporate growth factors loaded chitosan nanoparticles into electrospun fibrous scaffolds for bioactivity retention and cartilage regeneration.
Abstract Growth factor is an essential ingredient to regulate mesenchymal stem cells (MSCs) chondrogenic differentiation in cartilage tissue engineering. However, non-osteochondral specification, short plasma half time and bioactivity loss restrict growth factor's application. Thus, novel chondrogenic growth factors, specifically target osteochondral lineage cells, that can be sustained release and bioactivity protected to exert functions continually and effectively have attracted increasing researchers' interest. To achieve these goals, chitosan nanoparticles and electrospun fiber scaffolds were used as dual rele...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Wang C, Hou W, Guo X, Li J, Hu T, Qiu M, Liu S, Mo X, Liu X Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Antimicrobial and antioxidant potentials of biosynthesized colloidal zinc oxide nanoparticles for a fortified cold cream formulation: A potent nanocosmeceutical application.
Abstract Nanocosmeceuticals are promising applications of nanotechnology in personal care industries. Zinc oxide is an inorganic material that is non-toxic and skin compatible with self-cleansing and microbicidal properties. Herein, exploitation of colloidal zinc oxide nanoparticles (ZnONps) as potent biomaterial for a topical formulation of cosmetic and dermatological significance is employed. ZnONps were green synthesized using environmentally benign Adhatoda vasica leaf extract and characterized by UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic l...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: S S, H LJK, K R, M S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment.
Abstract Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surfa...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Chen TF, Siow KS, Ng PY, Majlis BY Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Construction of poly(lactic-co-glycolic acid)/ZnO nanorods/Ag nanoparticles hybrid coating on Ti implants for enhanced antibacterial activity and biocompatibility.
Abstract Poly(lactic-co-glycolic acid)/Ag/ZnO nanorods coating were successfully prepared on the surface of Ti metallic implants using a hydrothermal method and subsequent spin-coating of mixtures of poly(lactic-co-glycolic acid) and silver nanoparticles. The poly(lactic-co-glycolic acid)/Ag/ZnO nanorods coating exhibited excellent antibacterial efficacy of over 96% against both Staphylococcus aureus and Escherichia coli when the initial content of Ag nanoparticles was over 3wt%. In addition, the release of both silver and zinc could last for over a hundred days due to the enwrapping of poly(lactic-co-glycolic aci...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Xiang Y, Li J, Liu X, Cui Z, Yang X, Yeung KWK, Pan H, Wu S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Substitution of strontium and boron into hydroxyapatite crystals: Effect on physicochemical properties and biocompatibility with human Wharton-Jelly stem cells.
Abstract Hydroxyapatite (HA) enriched with strontium and boron ions was synthesized using two different methods: the precipitation method (Sr,B-HAw) and the dry method (Sr,B-HAd). Additionally, for the sake of comparison, the "pure" unsubstituted HA was prepared together with HAs substituted only with one type of a foreign ion. The obtained materials were subjected to physicochemical analysis with the use of various analytical methods, such as powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), inductively coupled plasma optical emission spectroscopy (ICP-OES), Fourier transform infr...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Kolmas J, Velard F, Jaguszewska A, Lemaire F, Kerdjoudj H, Gangloff SC, Kaflak A Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Glucosamine-modified polyethylene glycol hydrogel-mediated chondrogenic differentiation of human mesenchymal stem cells.
In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture. The GA-modified PEG hydrogels promoted the chondrogenesis of hBMSCs, particularly in the concentration of 5mM and 10mM. The subcutaneous transplantation of 10mM GA-modified hydrogels with hBMSCs formed cartilage-like blocks in vivo for 8weeks. Importantly, with glucosamine increase, the modified hydrogels down-regulated the fibrosis and hypertrophic cartilage markers ...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Yao H, Xue J, Wang Q, Xie R, Li W, Liu S, Cai J, Qin D, Wang DA, Ren L Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Evaluation of electrospun biomimetic substrate surface-decorated with nanohydroxyapatite precipitation for osteoblasts behavior.
Abstract To engineer bone tissue, it is crucial to design scaffolds with micro- and nano-sized architecture imitating approximate hierarchical structure of native bone, and afford desirable biological properties by introducing biocompatible polymers and bioceramics into the scaffolds. Here, a novel scaffold consisting of poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV)/polyaspartic acid (PAA) was fabricated by electrospinning and nano-hydroxyapatite (nHA) was deposited by calcium-phosphate dipping process for bone tissue regeneration. Characterization of the prepared nanofibers revealed the formation of definite...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Zhang S, Jiang G, Prabhakaran MP, Qin X, Ramakrishna S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications.
In this study, silver doped porous β-tricalcium phosphate (β-TCP) scaffolds were engineered using liquid porogen based method with the goal of meeting these requirements. Silver was added to the β-TCP at three different dopant levels: 0.5wt% Ag2O, 1wt% Ag2O and 2wt% Ag2O. Immersion in pH5 acetate buffer over a 60day period resulted in a total cumulative ion release between 32 and 54μM for dense control scaffolds, and between 80 and 90μM for porous scaffolds. Porosity increased the dissolution rate of the scaffolds by a factor of 2. Human osteoblast cell lines were grown on the scaffolds to measure cyto...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Hoover S, Tarafder S, Bandyopadhyay A, Bose S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Design of a hybrid biomaterial for tissue engineering: Biopolymer-scaffold integrated with an autologous hydrogel carrying mesenchymal stem-cells.
Acevedo CA Abstract Biologically active biomaterials as biopolymers and hydrogels have been used in medical applications providing favorable results in tissue engineering. In this research, a wound dressing device was designed by integration of an autologous clot hydrogel carrying mesenchymal stem-cells onto a biopolymeric scaffold. This hybrid biomaterial was tested in-vitro and in-vivo, and used in a human clinical case. The biopolymeric scaffold was made with gelatin, chitosan and hyaluronic acid, using a freeze-drying method. The scaffold was a porous material which was designed evaluating both physical prope...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Weinstein-Oppenheimer CR, Brown DI, Coloma R, Morales P, Reyna-Jeldes M, Díaz MJ, Sánchez E, Acevedo CA Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Encapsulation of methotrexate and cyclophosphamide in interpolymer complexes formed between poly acrylic acid and poly ethylene glycol on multi-walled carbon nanotubes as drug delivery systems.
Abstract By combining the advantage of multi-walled carbon nanotubes (MWCNTs) and interpolymer complexes, we synthesis a new carrier system consist of poly(acrylic acid)/poly(ethylene glycol)/carbon nanotube (PAA/PEG/CNT). Then, Methotrexate (MTX) and Cyclophosphamide (CPP) were loaded on PAA/PEG/CNT, and the physicochemical properties of nanoparticles characterized by Infrared spectroscopy (IR), Scanning Electron Microscopy (SEM), Thermo Gravimetric Analysis (TGA) and Nuclear Magnetic Resonance (NMR). In the second part after efficiency determination of loaded drugs, in vitro drug release study was examined with ...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Azqhandi MHA, Farahani BV, Dehghani N Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Polyurethane-silica hybrid foams from a one-step foaming reaction, coupled with a sol-gel process, for enhanced wound healing.
Abstract Polyurethane (PU)-based dressing foams have been widely used due to their excellent water absorption capability, optimal mechanical properties, and unequaled economic advantage. However, the low bioactivity and poor healing capability of PU limit the applications of PU dressings in complex wound healing cases. To resolve this problem, this study was carried out the hybridization of bioactive silica nanoparticles with PU through a one-step foaming reaction that is coupled with the sol-gel process. The hybridization with silica did not affect the intrinsically porous microstructure of PU foams with silica c...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Song EH, Jeong SH, Park JU, Kim S, Kim HE, Song J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Binary functional porous multi mineral-substituted apatite nanoparticles for reducing osteosarcoma colonization and enhancing osteoblast cell proliferation.
Abstract We investigated the use of combined nanoparticles in bone replacement for patients with bone cancer. Bismuth (Bi) is known to have antitumor effects, and the inclusion of Bi in bioactive mineral (M)-substituted hydroxyapatite (M-HAP) may provide anti-cancer properties implant resources. A series of Bi-substituted M-HAP (Bi-M-HAP) nanoparticles with various Bi concentrations was synthesized via the ultrasound irradiation method. The nanoparticles were examined using physicochemical strategies. The cell-substance interface of the nanoparticles was observed in vitro with human osteosarcoma cells and cell mul...
Source: Appl Human Sci - June 22, 2017 Category: Physiology Authors: Govindaraj D, Govindasamy C, Rajan M Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Hybrid ZnPc@TiO2 nanostructures for targeted photodynamic therapy, bioimaging and doxorubicin delivery.
In conclusion, prepared hybrid nanostructures exhibit high potential as selective bioimaging agents next to their photodynamic activity and drug delivery ability. PMID: 28575942 [PubMed - in process] (Source: Appl Human Sci)
Source: Appl Human Sci - June 5, 2017 Category: Physiology Authors: Flak D, Yate L, Nowaczyk G, Jurga S Tags: Mater Sci Eng C Mater Biol Appl Source Type: research