Perforated Appendicitis in Children: Management, Microbiology, and Antibiotic Stewardship
Paediatr Drugs. 2024 Apr 24. doi: 10.1007/s40272-024-00630-0. Online ahead of print.ABSTRACTAlthough appendicitis has been described for more than 300 years, its optimal management remains a topic of active investigation. Acute appendicitis is the most common cause of peritonitis in children, and rates of perforated appendicitis are much higher in children than in adults. Increased risk for perforated appendicitis in children is related to a delay in diagnosis due to age, size, access to care, and more. Surgical options include immediate appendectomy versus nonoperative management with intravenous antibiotics ± a drainage...
Source: Paediatric Drugs - April 23, 2024 Category: Pediatrics Authors: Katherine B Snyder Catherine J Hunter Christie L Buonpane Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Enzyme-Triggered Polyelectrolyte Complex for Responsive Delivery of α-Helical Polypeptides to Optimize Antibacterial Therapy
In this study, we present an enzyme-responsive polyelectrolyte complex micelles (PTPMN) with α-helical cationic polypeptide as a coacervate-core for the treatment of Escherichia coli (E. coli) infection. The complex was constructed through electrostatic interaction between cationic poly(glutamic acid) derivatives and phosphorylation-modified poly(ethylene glycol)-b-poly(tyrosine) (PEG-b-PPTyr) by directly dissolving them in aqueous solution. The cationic polypeptide adopted α-helical structure and demonstrated excellent broad-spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria, with a mi...
Source: Biomacromolecules - April 23, 2024 Category: Biochemistry Authors: Liuxuan Li Ruoxue Wang Bo Zhao Bowen Yin Huijuan Zhang Chunyong Liang Xiuli Hu Source Type: research
Paper-based loop-mediated isothermal amplification and CRISPR integrated platform for on-site nucleic acid testing of pathogens
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates the need for thermal cycling, resulting in simplified instrumentation, and the CRISPR-associated protein (Cas 12a) system eliminates false positive signals from LAMP products, resulting in highly selective and sensitive assays. We optimized the assay to perform both amplification and detection entir...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Anindita Sen Manaswini Masetty Sasanka Weerakoon Calum Morris Jagjit S Yadav Senu Apewokin Jennifer Trannguyen Murray Broom Aashish Priye Source Type: research
Synthesis and biological properties of novel glucose-based macromolecular architectures
Int J Biol Macromol. 2024 Apr 21:131724. doi: 10.1016/j.ijbiomac.2024.131724. Online ahead of print.ABSTRACTThe emergence of novel well-defined biological macromolecular architectures containing fluorine moieties displaying superior functionalities can satisfactorily address many biomedical challenges. In this research, ABA- and AB-type glucose-based biological macromolecules were synthesized using acryl-2,3,4,6-tetra-O-acetyl-D-glucopyranoside with pentafluorophenyl (FPM), pentafluorobenzyl (FBM), phenyl (PM) and benzyl (BM) methacrylate-based macro-RAFT agents following RAFT polymerization. The macro-RAFT agents and the ...
Source: International Journal of Biological Macromolecules - April 23, 2024 Category: Biochemistry Authors: Nagamalleswara Rao Indla Yeggada Maruthi Reetika Rawat T Sandeep Kumar N Ramesh Reddy Manu Sharma Tejraj M Aminabhavi Raghava Reddy Kakarla Annadanam V Sesha Sainath Source Type: research
Efficient Synthetic Access to Stable Isotope Labelled Pseudouridine Phosphoramidites for RNA NMR Spectroscopy
Chemistry. 2024 Apr 23:e202401193. doi: 10.1002/chem.202401193. Online ahead of print.ABSTRACTHere we report the efficient synthetic access to 13C/15N-labelled pseudouridine phosphoramidites, which were incorporated into a binary H/ACA box guide RNA/product complex comprising 77 nucleotides (nts) in total and into a 75 nt E. coli tRNAGly. The stable isotope (SI) labelled pseudouridines were produced via a highly efficient chemo-enzymatic synthesis. 13C/15N labelled uracils were produced via chemical synthesis and enzymatically converted to 5'-monophosphate pseudouridine (ΨMP) by using YeiN, a Ψ-5'-monophosphate C-glycosi...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: David Gl änzer Martin Pfeiffer Andrej Ribar Ricarda Zeindl Martin Tollinger Bernd Nidetzky Christoph Kreutz Source Type: research
Application of Mono and Trinuclear Cyclometalated Iridium (III) Complexes in Differential Bacterial Imaging and Antimicrobial Photodynamic therapy
Chemistry. 2024 Apr 23:e202400646. doi: 10.1002/chem.202400646. Online ahead of print.ABSTRACTThe application of transition metal complexes for antimicrobial photodynamic therapy (PDT) has emerged as an attractive alternative in mitigating a broad range of bacterial pathogens, including multidrug-resistant pathogens. In view of their photostability, long excited-state lifetimes, and tunable emission properties, transition metal complexes also contribute as bioimaging agents. In the present work, we designed mono and trinuclear cyclometalated iridium(III) complexes to explore their imaging application and antibacterial pote...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: Bishnu Das Prakash Biswas Amirul Islam Mallick Parna Gupta Source Type: research
Greenspace and human microbiota: A systematic review
CONCLUSION: The current evidence suggests that greenspace exposure may diversify gut and skin microbiota and alter their composition to healthier profiles. These findings would be helpful in uncovering the potential mechanisms underlying greenspace and human health and in promoting a healthier profile of human microbiota.PMID:38653130 | DOI:10.1016/j.envint.2024.108662 (Source: Environment International)
Source: Environment International - April 23, 2024 Category: Environmental Health Authors: Yi-Dan Zhang Gang-Long Zhou Lu Wang Matthew H E M Browning Iana Markevych Joachim Heinrich Luke D Knibbs Tianyu Zhao Yizhen Ding Shi Chen Kang-Kang Liu Payam Dadvand Guang-Hui Dong Bo-Yi Yang Source Type: research
Perforated Appendicitis in Children: Management, Microbiology, and Antibiotic Stewardship
Paediatr Drugs. 2024 Apr 24. doi: 10.1007/s40272-024-00630-0. Online ahead of print.ABSTRACTAlthough appendicitis has been described for more than 300 years, its optimal management remains a topic of active investigation. Acute appendicitis is the most common cause of peritonitis in children, and rates of perforated appendicitis are much higher in children than in adults. Increased risk for perforated appendicitis in children is related to a delay in diagnosis due to age, size, access to care, and more. Surgical options include immediate appendectomy versus nonoperative management with intravenous antibiotics ± a drainage...
Source: Paediatric Drugs - April 23, 2024 Category: Pediatrics Authors: Katherine B Snyder Catherine J Hunter Christie L Buonpane Source Type: research
Molecules, Vol. 29, Pages 1908: Volatile Fatty Acids Effective as Antibacterial Agents against Three Enteric Bacteria during Mesophilic Anaerobic Incubation
T. Semple
The antibacterial effects of a selection of volatile fatty acids (acetic, propionic, butyric, valeric, and caproic acids) relevant to anaerobic digestion were investigated at 1, 2 and 4 g/L. The antibacterial effects were characterised by the dynamics of Enterococcus faecalis NCTC 00775, Escherichia coli JCM 1649 and Klebsiella pneumoniae A17. Mesophilic anaerobic incubation to determine the minimum bactericidal concentration (MBC) and median lethal concentration of the VFAs was carried out in Luria Bertani broth at 37 °C for 48 h. Samples collected at times 0, 3, 6, 24 and 48 h were used to moni...
Source: Molecules - April 23, 2024 Category: Chemistry Authors: Saanu Victoria Otite Alfonso Jos é Lag-Brotons Lawrence I. Ezemonye Alastair D. Martin Roger W. Pickup Kirk T. Semple Tags: Article Source Type: research
Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea
CONCLUSIONS: Childhood diarrhea can lead to increasingly severe nutrient deficiencies. Steatorrhea is the hallmark of malabsorption, and a stool test, such as the acid steatocrit, can be routinely used as a laboratory tool for the semi-quantitative evaluation of fat malabsorption in diarrheic children.PMID:38648349 | DOI:10.7705/biomedica.6913 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - April 22, 2024 Category: International Medicine & Public Health Authors: Adson Santos Martins Samara Alves Santos Cl áudia Alves da Silva Lisboa T ânia Fraga Barros Tereza Cristina Medrado Ribeiro Hugo Da Costa-Ribeiro Ângela Peixoto de Mattos Patr ícia Silva de Almeida Mendes Carlos Maur ício Cardeal Mendes Edna L úcia Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea
CONCLUSIONS: Childhood diarrhea can lead to increasingly severe nutrient deficiencies. Steatorrhea is the hallmark of malabsorption, and a stool test, such as the acid steatocrit, can be routinely used as a laboratory tool for the semi-quantitative evaluation of fat malabsorption in diarrheic children.PMID:38648349 | DOI:10.7705/biomedica.6913 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - April 22, 2024 Category: International Medicine & Public Health Authors: Adson Santos Martins Samara Alves Santos Cl áudia Alves da Silva Lisboa T ânia Fraga Barros Tereza Cristina Medrado Ribeiro Hugo Da Costa-Ribeiro Ângela Peixoto de Mattos Patr ícia Silva de Almeida Mendes Carlos Maur ício Cardeal Mendes Edna L úcia Source Type: research
Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study
In this study, we have explored the role of Glu78 in the regulation of pH by Ec-NhaA. Although we have created various mutants, E78C has shown a considerable effect on the stoichiometry of NhaA and presented comparable phenotypes. The ITC experiment has shown the binding of ~ 5 protons in response to the transport of one lithium ion. The phenotype analysis on selective medium showed a significant expression compared to WT Ec-NhaA. This represents the importance of Glu78 in transporting the H+ across the membrane where a single mutation with Cys amino acid alters the number of H+ significantly maintaining the activity of th...
Source: European Biophysics Journal : EBJ - April 22, 2024 Category: Physics Authors: Anuradha Yadav Dinesh Kumar Manish Dwivedi Source Type: research
