Binding of Proteins to Copolymers of Varying Charges and Hydrophobicity: A Molecular Mechanism and Computational Strategies
In this study, we found that with more copolymer charges, the protein coverage increases for copolymers of low hydrophobicity and decreases of high hydrophobicity, which is explained by the distribution and size of various functional patches on the VD in loading those copolymers. Employing a coverage-dependent Langmuir model, we propose a simulation protocol to address the full profile of the copolymer binding free energy through the fit to the simulated binding isotherm. The obtained results correlate well with those from the BLI experiment, indicating the significance of this method for the rational design of the copolym...
Source: Biomacromolecules - September 27, 2022 Category: Biochemistry Authors: Xiao Xu Tong Zhang Stefano Angioletti-Uberti Yongqin Lv Source Type: research

Heat-Stimuli Shape Memory Effect of Poly ( ε-Caprolactone)-Cellulose Acetate Composite Tubular Scaffolds
In this study, seven types of poly(-caprolactone)-cellulose acetate (PCL-CA) composite nanofiber membranes were prepared with different proportions of PCL and CA. The adhesion and growth of Mc3t3-e1 cells were considered to confirm the in vitro cytocompatibility of PCL-CA membranes. A smooth stainless-steel mandrel with a diameter of 4 mm was used to roll up the prepared nanofiber membranes to produce the tubular scaffold with 50 °C hot water. The tubular scaffolds were subjected to axial and circumferential tensile tests. The mechanical performance of the PCL-CA tubular scaffold could be improved by increasing the layers...
Source: Biomacromolecules - September 27, 2022 Category: Biochemistry Authors: Hao Wang Hong Xia Zhenzhen Xu Baoji Hu Toshiaki Natsuki Qing-Qing Ni Source Type: research

Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device
Biomacromolecules. 2022 Sep 27. doi: 10.1021/acs.biomac.2c00815. Online ahead of print.ABSTRACTIn traditional blood-contacting medical devices, infection and thrombosis are easily formed on the surface of the materials. In addition, inflammation is also a clinical complication that cannot be ignored. More importantly, there is a mutually promoting relationship between the inflammatory response and the infection as well as thrombosis. In this work, we propose a self-adaptive anti-inflammatory coating strategy combined with anti-infection and anticoagulant capacity, which was accomplished based on nano-Ag particles and dexam...
Source: Biomacromolecules - September 27, 2022 Category: Biochemistry Authors: Yuan Wei Jingze Liu Gongyan Liu Shuai Gao Dimeng Wu Li Yang Rifang Luo Fanjun Zhang Yunbing Wang Source Type: research

Covalent Crosslinking of Colloidal Cellulose Nanocrystals for Multifunctional Nanostructured Hydrogels with Tunable Physicochemical Properties
Biomacromolecules. 2022 Sep 27. doi: 10.1021/acs.biomac.2c00417. Online ahead of print.ABSTRACTCellulose nanocrystals (CNCs) have shown promise for the development of multifunctional materials for many research communities, ranging from bioresource engineering and biomedical engineering to materials science and engineering. However, accessible hydroxyl (OH) groups on the surface of colloidal CNCs at the (11̅0)β/(100)α and (110)β/(010)α facets and the intermolecular hydrogen bonding (H-bonds) between these OH groups account for the instability of self-assembled CNC structures in moist environments, limiting their pract...
Source: Biomacromolecules - September 27, 2022 Category: Biochemistry Authors: Joseph Batta-Mpouma Gurshagan Kandhola Joshua Sakon Jin-Woo Kim Source Type: research

Copolymer-Functionalized Cellulose Nanocrystals as a pH- and NIR-Triggered Drug Carrier for Simultaneous Photothermal Therapy and Chemotherapy of Cancer Cells
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00770. Online ahead of print.ABSTRACTAs a class of biocompatible and biodegradable naturally derived nanomaterials, cellulose nanocrystals (CNCs) with diverse surface functionalization have aroused considerable attention for a range of biomedical applications in drug or gene delivery, as a fluorescent nanoprobe, in cancer targeting, and in photothermal cancer therapy, among others. Herein, we construct the copolymer-functionalized CNCs as a pH- and near-infrared (NIR)-triggered drug carrier for simultaneous photothermal therapy and chemotherapy of cancer cells. Poly...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: Jianchang Xu Jing Zhang Fusheng Zhang Lijuan Zhang Source Type: research

Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials
Biomacromolecules. 2022 Sep 23. doi: 10.1021/acs.biomac.2c00544. Online ahead of print.ABSTRACTConstructing protein-network materials that exhibit physicochemical and mechanical properties of individual protein constituents requires molecular cross-linkers with specificity and stability. A well-known example involves specific chemical fusion of a four-arm polyethylene glycol (tetra-PEG) to desired proteins with secondary cross-linkers. However, it is necessary to investigate tetra-PEG-like biomolecular cross-linkers that are genetically fused to the proteins, simplifying synthesis by removing additional conjugation and pur...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: David S Knoff Samuel Kim Kareen A Fajardo Cortes Jocelyne Rivera Marcus V J Cathey Dallas Altamirano Christopher Camp Minkyu Kim Source Type: research

Copolymer-Functionalized Cellulose Nanocrystals as a pH- and NIR-Triggered Drug Carrier for Simultaneous Photothermal Therapy and Chemotherapy of Cancer Cells
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00770. Online ahead of print.ABSTRACTAs a class of biocompatible and biodegradable naturally derived nanomaterials, cellulose nanocrystals (CNCs) with diverse surface functionalization have aroused considerable attention for a range of biomedical applications in drug or gene delivery, as a fluorescent nanoprobe, in cancer targeting, and in photothermal cancer therapy, among others. Herein, we construct the copolymer-functionalized CNCs as a pH- and near-infrared (NIR)-triggered drug carrier for simultaneous photothermal therapy and chemotherapy of cancer cells. Poly...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: Jianchang Xu Jing Zhang Fusheng Zhang Lijuan Zhang Source Type: research

Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials
Biomacromolecules. 2022 Sep 23. doi: 10.1021/acs.biomac.2c00544. Online ahead of print.ABSTRACTConstructing protein-network materials that exhibit physicochemical and mechanical properties of individual protein constituents requires molecular cross-linkers with specificity and stability. A well-known example involves specific chemical fusion of a four-arm polyethylene glycol (tetra-PEG) to desired proteins with secondary cross-linkers. However, it is necessary to investigate tetra-PEG-like biomolecular cross-linkers that are genetically fused to the proteins, simplifying synthesis by removing additional conjugation and pur...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: David S Knoff Samuel Kim Kareen A Fajardo Cortes Jocelyne Rivera Marcus V J Cathey Dallas Altamirano Christopher Camp Minkyu Kim Source Type: research

Copolymer-Functionalized Cellulose Nanocrystals as a pH- and NIR-Triggered Drug Carrier for Simultaneous Photothermal Therapy and Chemotherapy of Cancer Cells
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00770. Online ahead of print.ABSTRACTAs a class of biocompatible and biodegradable naturally derived nanomaterials, cellulose nanocrystals (CNCs) with diverse surface functionalization have aroused considerable attention for a range of biomedical applications in drug or gene delivery, as a fluorescent nanoprobe, in cancer targeting, and in photothermal cancer therapy, among others. Herein, we construct the copolymer-functionalized CNCs as a pH- and near-infrared (NIR)-triggered drug carrier for simultaneous photothermal therapy and chemotherapy of cancer cells. Poly...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: Jianchang Xu Jing Zhang Fusheng Zhang Lijuan Zhang Source Type: research

Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials
Biomacromolecules. 2022 Sep 23. doi: 10.1021/acs.biomac.2c00544. Online ahead of print.ABSTRACTConstructing protein-network materials that exhibit physicochemical and mechanical properties of individual protein constituents requires molecular cross-linkers with specificity and stability. A well-known example involves specific chemical fusion of a four-arm polyethylene glycol (tetra-PEG) to desired proteins with secondary cross-linkers. However, it is necessary to investigate tetra-PEG-like biomolecular cross-linkers that are genetically fused to the proteins, simplifying synthesis by removing additional conjugation and pur...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: David S Knoff Samuel Kim Kareen A Fajardo Cortes Jocelyne Rivera Marcus V J Cathey Dallas Altamirano Christopher Camp Minkyu Kim Source Type: research

Copolymer-Functionalized Cellulose Nanocrystals as a pH- and NIR-Triggered Drug Carrier for Simultaneous Photothermal Therapy and Chemotherapy of Cancer Cells
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00770. Online ahead of print.ABSTRACTAs a class of biocompatible and biodegradable naturally derived nanomaterials, cellulose nanocrystals (CNCs) with diverse surface functionalization have aroused considerable attention for a range of biomedical applications in drug or gene delivery, as a fluorescent nanoprobe, in cancer targeting, and in photothermal cancer therapy, among others. Herein, we construct the copolymer-functionalized CNCs as a pH- and near-infrared (NIR)-triggered drug carrier for simultaneous photothermal therapy and chemotherapy of cancer cells. Poly...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: Jianchang Xu Jing Zhang Fusheng Zhang Lijuan Zhang Source Type: research

Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials
Biomacromolecules. 2022 Sep 23. doi: 10.1021/acs.biomac.2c00544. Online ahead of print.ABSTRACTConstructing protein-network materials that exhibit physicochemical and mechanical properties of individual protein constituents requires molecular cross-linkers with specificity and stability. A well-known example involves specific chemical fusion of a four-arm polyethylene glycol (tetra-PEG) to desired proteins with secondary cross-linkers. However, it is necessary to investigate tetra-PEG-like biomolecular cross-linkers that are genetically fused to the proteins, simplifying synthesis by removing additional conjugation and pur...
Source: Biomacromolecules - September 23, 2022 Category: Biochemistry Authors: David S Knoff Samuel Kim Kareen A Fajardo Cortes Jocelyne Rivera Marcus V J Cathey Dallas Altamirano Christopher Camp Minkyu Kim Source Type: research

Biomolecular Complexation on the "Wrong Side": A Case Study of the Influence of Salts and Sugars on the Interactions between Bovine Serum Albumin and Sodium Polystyrene Sulfonate
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00933. Online ahead of print.ABSTRACTIn the protein purification, drug delivery, food industry, and biotechnological applications involving protein-polyelectrolyte complexation, proper selection of co-solutes and solution conditions plays a crucial role. The onset of (bio)macromolecular complexation occurs even on the so-called "wrong side" of the protein isoionic point where both the protein and the polyelectrolyte are net like-charged. To gain mechanistic insights into the modulatory role of salts (NaCl, NaBr, and NaI) and sugars (sucrose and sucralose) in protein...
Source: Biomacromolecules - September 22, 2022 Category: Biochemistry Authors: Matja ž Simončič Jozef Hritz Miha Luk šič Source Type: research

Tuning the Cell-Adhesive Properties of Two-Component Hybrid Hydrogels to Modulate Cancer Cell Behavior, Metastasis, and Death Pathways
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00733. Online ahead of print.ABSTRACTThis work presents a polysaccharide and protein-based two-component hybrid hydrogel integrating the cell-adhesive gelatin-tyramine (G-Tyr) and nonadhesive hyaluronic acid-tyramine (HA-Tyr) through enzyme-mediated oxidative coupling reaction. The resulting HA-Tyr/G-Tyr hydrogel reflects the precise chemical and mechanical features of the cancer extracellular matrix and is able to tune cancer cell adhesion upon switching the component ratio. The cells form quasi-spheroids on HA-Tyr rich hydrogels, while they tend to form an invasiv...
Source: Biomacromolecules - September 22, 2022 Category: Biochemistry Authors: Melis Isik Babatunde O Okesola Cemil Can Eylem Engin Kocak Emirhan Nemutlu Emel Emregul Matteo D'Este Burak Derkus Source Type: research

Modulating Nanodroplet Formation En Route to Fibrillization of Amyloid Peptides with Designed Flanking Sequences
Biomacromolecules. 2022 Sep 22. doi: 10.1021/acs.biomac.2c00642. Online ahead of print.ABSTRACTSoluble oligomers populating early amyloid aggregation can be regarded as nanodroplets of liquid-liquid phase separation (LLPS). Amyloid peptides typically contain hydrophobic aggregation-prone regions connected by hydrophilic linkers and flanking sequences, and such a sequence hydropathy pattern drives the formation of supramolecular structures in the nanodroplets and modulates subsequent fibrillization. Here, we studied LLPS and fibrillization of coarse-grained amyloid peptides with increasing flanking sequences. Nanodroplets a...
Source: Biomacromolecules - September 22, 2022 Category: Biochemistry Authors: Yanting Xing Nicholas Andrikopoulos Zhenzhen Zhang Yunxiang Sun Pu Chun Ke Feng Ding Source Type: research