Correction to "Optimizing pDNA Lipo-polyplexes: A Balancing Act between Stability and Cargo Release"
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c01502. Online ahead of print.NO ABSTRACTPMID:34854679 | DOI:10.1021/acs.biomac.1c01502 (Source: Biomacromolecules)
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Simone Berger Ana Krha č Levačić Elisa H örterer Ulrich Wilk Teoman Benli-Hoppe Yanfang Wang Özgür Öztürk Jie Luo Ernst Wagner Source Type: research

Hypothermic Stem Cell Storage Using a Polypeptide Thermogel
We report a polypeptide-based thermogel as a new tool for hypothermic storage of stem cells at ambient temperature (25 °C). Stem cells were suspended in the sol state (10 °C) of an aqueous poly(ethylene glycol)-poly(l-alanine) (PEG-PA) solution (4.0 wt %) in phosphate-buffered saline (PBS), which turned into a stem cell-incorporated gel by a heat-induced sol-to-gel transition. The cell harvesting procedure from the thermogels was simply performed through a gel-to-sol transition by diluting and cooling the system. More than 99% of stem cells died in PBS and Pluronic F127 thermogel (control thermogel) when the cells ...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Zhengyu Piao Jin Kyung Park So-Jung Park Byeongmoon Jeong Source Type: research

Genetic and Covalent Protein Modification Strategies to Facilitate Intracellular Delivery
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c00745. Online ahead of print.ABSTRACTProtein-based therapeutics represent a rapidly growing segment of approved disease treatments. Successful intracellular delivery of proteins is an important precondition for expanded in vivo and in vitro applications of protein therapeutics. Direct modification of proteins and peptides for improved cytosolic translocation are a promising method of increasing delivery efficiency and expanding the viability of intracellular protein therapeutics. In this Review, we present recent advances in both synthetic and genetic protein modific...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Justin M Horn Allie C Obermeyer Source Type: research

Intrinsically Fluorescent Oligomeric Cytotoxic Conjugates Toxic for FGFR1-Overproducing Cancers
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c01280. Online ahead of print.ABSTRACTFibroblast growth factor receptor 1 (FGFR1) is an integral membrane protein that transmits prolife signals through the plasma membrane. Overexpression of FGFR1 has been reported in various tumor types, and therefore, this receptor constitutes an attractive molecular target for selective anticancer therapies. Here, we present a novel system for generation of intrinsically fluorescent, self-assembling, oligomeric cytotoxic conjugates with high affinity and efficient internalization targeting FGFR1. In our approach, we employed FGF1 ...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Natalia Por ębska Agata Knapik Marta Po źniak Mateusz Adam Krzy ścik Ma łgorzata Zakrzewska Jacek Otlewski Łukasz Opaliński Source Type: research

Distribution and Quantification of Diverse Functional Groups on Phosphorylated Nanocellulose Surfaces
In this study, we investigated the chemical structures and distribution of ionic functional groups on the phosphorylated CNF surfaces via liquid-state nuclear magnetic resonance measurements of colloidal dispersion. In addition to the monophosphate group, polyphosphate groups and cross-linked phosphate groups were introduced in the phosphorylated CNFs. The proportion of polyphosphate groups increased as the phosphorylation time increased, reaching ∼30% of all phosphate groups. Only a small amount of cross-linked phosphate groups existed in the phosphorylated CNF after a prolonged reaction time. Furthermore, phosphoryla...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Mengchen Zhao Shuji Fujisawa Tsuguyuki Saito Source Type: research

Correction to "Optimizing pDNA Lipo-polyplexes: A Balancing Act between Stability and Cargo Release"
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c01502. Online ahead of print.NO ABSTRACTPMID:34854679 | DOI:10.1021/acs.biomac.1c01502 (Source: Biomacromolecules)
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Simone Berger Ana Krha č Levačić Elisa H örterer Ulrich Wilk Teoman Benli-Hoppe Yanfang Wang Özgür Öztürk Jie Luo Ernst Wagner Source Type: research

Hypothermic Stem Cell Storage Using a Polypeptide Thermogel
We report a polypeptide-based thermogel as a new tool for hypothermic storage of stem cells at ambient temperature (25 °C). Stem cells were suspended in the sol state (10 °C) of an aqueous poly(ethylene glycol)-poly(l-alanine) (PEG-PA) solution (4.0 wt %) in phosphate-buffered saline (PBS), which turned into a stem cell-incorporated gel by a heat-induced sol-to-gel transition. The cell harvesting procedure from the thermogels was simply performed through a gel-to-sol transition by diluting and cooling the system. More than 99% of stem cells died in PBS and Pluronic F127 thermogel (control thermogel) when the cells ...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Zhengyu Piao Jin Kyung Park So-Jung Park Byeongmoon Jeong Source Type: research

Genetic and Covalent Protein Modification Strategies to Facilitate Intracellular Delivery
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c00745. Online ahead of print.ABSTRACTProtein-based therapeutics represent a rapidly growing segment of approved disease treatments. Successful intracellular delivery of proteins is an important precondition for expanded in vivo and in vitro applications of protein therapeutics. Direct modification of proteins and peptides for improved cytosolic translocation are a promising method of increasing delivery efficiency and expanding the viability of intracellular protein therapeutics. In this Review, we present recent advances in both synthetic and genetic protein modific...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Justin M Horn Allie C Obermeyer Source Type: research

Intrinsically Fluorescent Oligomeric Cytotoxic Conjugates Toxic for FGFR1-Overproducing Cancers
Biomacromolecules. 2021 Dec 2. doi: 10.1021/acs.biomac.1c01280. Online ahead of print.ABSTRACTFibroblast growth factor receptor 1 (FGFR1) is an integral membrane protein that transmits prolife signals through the plasma membrane. Overexpression of FGFR1 has been reported in various tumor types, and therefore, this receptor constitutes an attractive molecular target for selective anticancer therapies. Here, we present a novel system for generation of intrinsically fluorescent, self-assembling, oligomeric cytotoxic conjugates with high affinity and efficient internalization targeting FGFR1. In our approach, we employed FGF1 ...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Natalia Por ębska Agata Knapik Marta Po źniak Mateusz Adam Krzy ścik Ma łgorzata Zakrzewska Jacek Otlewski Łukasz Opaliński Source Type: research

Distribution and Quantification of Diverse Functional Groups on Phosphorylated Nanocellulose Surfaces
In this study, we investigated the chemical structures and distribution of ionic functional groups on the phosphorylated CNF surfaces via liquid-state nuclear magnetic resonance measurements of colloidal dispersion. In addition to the monophosphate group, polyphosphate groups and cross-linked phosphate groups were introduced in the phosphorylated CNFs. The proportion of polyphosphate groups increased as the phosphorylation time increased, reaching ∼30% of all phosphate groups. Only a small amount of cross-linked phosphate groups existed in the phosphorylated CNF after a prolonged reaction time. Furthermore, phosphoryla...
Source: Biomacromolecules - December 2, 2021 Category: Biochemistry Authors: Mengchen Zhao Shuji Fujisawa Tsuguyuki Saito Source Type: research

Correction to "Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing"
Biomacromolecules. 2021 Dec 1. doi: 10.1021/acs.biomac.1c01487. Online ahead of print.NO ABSTRACTPMID:34851603 | DOI:10.1021/acs.biomac.1c01487 (Source: Biomacromolecules)
Source: Biomacromolecules - December 1, 2021 Category: Biochemistry Authors: Huinan Suo Mubashir Hussain Hua Wang Nuoya Zhou Juan Tao Hao Jiang Jintao Zhu Source Type: research

AIE-Based Fluorescent Triblock Copolymer Micelles for Simultaneous Drug Delivery and Intracellular Imaging
Biomacromolecules. 2021 Dec 1. doi: 10.1021/acs.biomac.1c01165. Online ahead of print.ABSTRACTFluorescent drug delivery systems have received increasing attention in cancer therapy because they combine drug delivery and bioimaging into a single platform. For example, polymers with aggregation-induced emission (AIE) fluorophores, such as tetraphenylethylene (TPE), have emerged as an elegant choice for drug delivery/bioimaging applications. In this work, we report one-pot sequential organocatalytic ring-opening polymerization of ε-caprolactone (CL) and ethylene oxide (EO) using TPE-(OH)2 as a difunctional initiator, ...
Source: Biomacromolecules - December 1, 2021 Category: Biochemistry Authors: Bhagyashree Kulkarni Somayah Qutub Viko Ladelta Niveen M Khashab Nikos Hadjichristidis Source Type: research

Correction to "Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing"
Biomacromolecules. 2021 Dec 1. doi: 10.1021/acs.biomac.1c01487. Online ahead of print.NO ABSTRACTPMID:34851603 | DOI:10.1021/acs.biomac.1c01487 (Source: Biomacromolecules)
Source: Biomacromolecules - December 1, 2021 Category: Biochemistry Authors: Huinan Suo Mubashir Hussain Hua Wang Nuoya Zhou Juan Tao Hao Jiang Jintao Zhu Source Type: research

Electrostatics Trigger Interfacial Self-Assembly of Bacterial Ice Nucleators
Biomacromolecules. 2021 Nov 30. doi: 10.1021/acs.biomac.1c01217. Online ahead of print.ABSTRACTIce active bacteria can catalyze water freezing at high subzero temperatures using ice nucleating proteins (INPs) located at their outer cell walls. INPs are the most effective ice nucleators known and are of significant interest for agriculture, climate research, and freeze/antifreeze technologies. The aggregation of INPs into large ice nucleation sites is a key step for effective ice nucleation. It has been proposed that ice active bacteria can drive the aggregation of INPs and thereby trigger ice nucleation. However, the mecha...
Source: Biomacromolecules - November 30, 2021 Category: Biochemistry Authors: Fani Madzharova Mikkel Bregnh øj Adam Simon Chatterley Kaja Borup L øvschall Taner Drace Lasse Sander Andersen Dreyer Thomas Boesen Tobias Weidner Source Type: research

Choline-Functionalized Supramolecular Copolymers: Toward Antimicrobial Activity against < em > Streptococcus pneumoniae < /em >
Biomacromolecules. 2021 Nov 30. doi: 10.1021/acs.biomac.1c01293. Online ahead of print.ABSTRACTDynamic binding events are key to arrive at functionality in nature, and these events are often governed by electrostatic or hydrophobic interactions. Synthetic supramolecular polymers are promising candidates to obtain biomaterials that mimic this dynamicity. Here, we created four new functional monomers based on the benzene-1,3,5-tricarboxamide (BTA) motif. Choline or atropine groups were introduced to obtain functional monomers capable of competing with the cell wall of Streptococcus pneumoniae for binding of essential choline...
Source: Biomacromolecules - November 30, 2021 Category: Biochemistry Authors: Marle E J Vleugels Silvia Varela-Aramburu Bas F M de Waal Sandra M C Schoenmakers Beatriz Maestro Anja R A Palmans Jes ús M Sanz E W Meijer Source Type: research

Poly(l-cysteine) Peptide Amphiphile Derivatives Containing Disulfide Bonds: Synthesis, Self-Assembly-Induced β-Sheet Nanostructures, pH/Reduction Dual Response, and Drug Release
Biomacromolecules. 2021 Nov 30. doi: 10.1021/acs.biomac.1c01324. Online ahead of print.ABSTRACTThree disulfide bond-containing peptide amphiphiles (PA1-3) with different lengths of alkyl tails (PA1 for C6, PA2 for C12, and PA3 for C18) were synthesized by ring-opening polymerization of α-amino acid N-carboxyanhydride followed by post-polymerization modification. The peptide segments were composed of 3-mercaptopropionic acid-modified poly(l-cysteine) [P(Cys-SS-CH2CH2COOH)]. We characterized the chemical structure and molecular parameters by 1H NMR, 13C NMR, gel permeation chromatography, Fourier transform infrared spe...
Source: Biomacromolecules - November 30, 2021 Category: Biochemistry Authors: Liang Dong Hui Chen Ting Liu Junming Zhu Min Yu Qingmei Yuan Source Type: research

Polymer-Mediated Cryopreservation of Bacteriophages
Biomacromolecules. 2021 Nov 30. doi: 10.1021/acs.biomac.1c01187. Online ahead of print.ABSTRACTBacteriophages (phages, bacteria-specific viruses) have biotechnological and therapeutic potential. To apply phages as pure or heterogeneous mixtures, it is essential to have a robust mechanism for transport and storage, with different phages having very different stability profiles across storage conditions. For many biologics, cryopreservation is employed for long-term storage and cryoprotectants are essential to mitigate cold-induced damage. Here, we report that poly(ethylene glycol) can be used to protect phages from cold dam...
Source: Biomacromolecules - November 30, 2021 Category: Biochemistry Authors: Huba L Marton Kathryn M Styles Peter Kilbride Antonia P Sagona Matthew I Gibson Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Mussel-Inspired Multiloop Polyethers for Antifouling Surfaces
This study provides a new platform for the development of versatile antifouling polymers with varying topologies.PMID:34818000 | DOI:10.1021/acs.biomac.1c01124 (Source: Biomacromolecules)
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Suebin Park Minseong Kim Jinwoo Park Woojin Choi Jinkee Hong Dong Woog Lee Byeong-Su Kim Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Mussel-Inspired Multiloop Polyethers for Antifouling Surfaces
This study provides a new platform for the development of versatile antifouling polymers with varying topologies.PMID:34818000 | DOI:10.1021/acs.biomac.1c01124 (Source: Biomacromolecules)
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Suebin Park Minseong Kim Jinwoo Park Woojin Choi Jinkee Hong Dong Woog Lee Byeong-Su Kim Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Mussel-Inspired Multiloop Polyethers for Antifouling Surfaces
This study provides a new platform for the development of versatile antifouling polymers with varying topologies.PMID:34818000 | DOI:10.1021/acs.biomac.1c01124 (Source: Biomacromolecules)
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Suebin Park Minseong Kim Jinwoo Park Woojin Choi Jinkee Hong Dong Woog Lee Byeong-Su Kim Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Mussel-Inspired Multiloop Polyethers for Antifouling Surfaces
This study provides a new platform for the development of versatile antifouling polymers with varying topologies.PMID:34818000 | DOI:10.1021/acs.biomac.1c01124 (Source: Biomacromolecules)
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Suebin Park Minseong Kim Jinwoo Park Woojin Choi Jinkee Hong Dong Woog Lee Byeong-Su Kim Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Mussel-Inspired Multiloop Polyethers for Antifouling Surfaces
This study provides a new platform for the development of versatile antifouling polymers with varying topologies.PMID:34818000 | DOI:10.1021/acs.biomac.1c01124 (Source: Biomacromolecules)
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Suebin Park Minseong Kim Jinwoo Park Woojin Choi Jinkee Hong Dong Woog Lee Byeong-Su Kim Source Type: research

Aldehyde-Functional Diblock Copolymer Nano-objects < em > via < /em > RAFT Aqueous Dispersion Polymerization
We report the rational design of aldehyde-functional sterically stabilized diblock copolymer nano-objects in aqueous solution via polymerization-induced self-assembly. More specifically, reversible addition-fragmentation chain transfer aqueous dispersion polymerization of 2-hydroxypropyl methacrylate is conducted using a water-soluble precursor block in which every methacrylic repeat unit contains a pendent oligo(ethylene glycol) side chain capped with a cis-diol unit. Systematic variation of the reaction conditions enables the construction of a pseudo-phase diagram, which ensures the reproducible targeting of pure spheres...
Source: Biomacromolecules - November 24, 2021 Category: Biochemistry Authors: Emma E Brotherton Mark J Smallridge Steven P Armes Source Type: research

Injectable and Biodegradable Chitosan Hydrogel-Based Drug Depot Contributes to Synergistic Treatment of Tumors
Biomacromolecules. 2021 Nov 23. doi: 10.1021/acs.biomac.1c01279. Online ahead of print.ABSTRACTCombined therapy provides a more effective method in the treatment of tumors and becomes a research hotspot. To improve treatment outcomes and reduce serious side effects, hydrogel-based local delivery was developed herein to form a drug depot in suit to eliminate tumors. Indocyanine green and imiquimod were coencapsulated in the novel temperature-sensitive chitosan hydrogel. After intratumoral injection of the hydrogel, indocyanine green that accumulated in the tumor area could induce thermal ablation of primary tumors under las...
Source: Biomacromolecules - November 23, 2021 Category: Biochemistry Authors: Enci Mei Cunguo Chen Chunxiao Li Xiaoxia Ding Jiashe Chen Qiaoer Xi Sen Zhou Jingjing Liu Zhiming Li Source Type: research

Gradient Diffusion Anisotropic Carboxymethyl Cellulose Hydrogels for Strain Sensors
Biomacromolecules. 2021 Nov 23. doi: 10.1021/acs.biomac.1c01003. Online ahead of print.ABSTRACTRecently, because of the unique properties of anisotropic and isotropic structures, there are more research studies on anisotropic hydrogels. We prepared a gradient anisotropic carboxymethyl cellulose hydrogel (CMC-Al3+) by directionally diffusing aluminum chloride solution. The orientation of carboxymethyl cellulose (CMC) chains is perpendicular to the direction of aluminum ion diffusion. The degree of cross-linking and orientation gradually decrease along the direction of aluminum ion diffusion. Compared with anisotropic hydrog...
Source: Biomacromolecules - November 23, 2021 Category: Biochemistry Authors: Kangwen Ouyang Jie Zhuang Chuchu Chen Xuerong Wang Mengting Xu Zhaoyang Xu Source Type: research

Investigating the Conformation of Surface-Adsorbed Proteins with Standing-Wave X-ray Fluorescence
Biomacromolecules. 2021 Nov 23. doi: 10.1021/acs.biomac.1c01136. Online ahead of print.ABSTRACTProtein adsorption to surfaces is at the heart of numerous technological and bioanalytical applications, but sometimes, it is also associated with medical risks. To deepen our insights into processes involving layers of surface-adsorbed proteins, high-resolution structural information is essential. Here, we use standing-wave X-ray fluorescence (SWXF) in combination with an optimized liquid-cell setup to investigate the underwater conformation of the random-coiled phosphoprotein β-casein adsorbed to hydrophilic and hydrophobi...
Source: Biomacromolecules - November 23, 2021 Category: Biochemistry Authors: Ernesto Scoppola Georgi G Gochev Jakub Drnec Linus Pithan Dmitri Novikov Emanuel Schneck Source Type: research

Photoreactive Cytosine-Functionalized Self-Assembled Micelles with Enhanced Cellular Uptake Capability for Efficient Cancer Chemotherapy
Biomacromolecules. 2021 Nov 22. doi: 10.1021/acs.biomac.1c01199. Online ahead of print.ABSTRACTDesign, fabrication, and control of photoreactive supramolecular macromers─which are composed of a thermoresponsive polymer backbone and photoreactive nucleobase end-groups─to achieve the desired physical-chemical performance and provide the high efficiency required for chemotherapy drug delivery purposes still present challenges. Herein, a difunctional cytosine-terminated supramolecular macromer was successfully obtained at high yield. UV-irradiation induces the formation of cytosine photodimers within the structure. The irr...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Abere Habtamu Manayia Fasih Bintang Ilhami Ai-Wei Lee Chih-Chia Cheng Source Type: research

Biological Activities and Emerging Roles of Lignin and Lignin-Based Products ─A Review
Biomacromolecules. 2021 Nov 21. doi: 10.1021/acs.biomac.1c00805. Online ahead of print.ABSTRACTBioactive substances, displaying excellent biocompatibility, chemical stability, and processability, could be extensively applied in biomedicine and tissue engineering. In recent years, plant-based bioactive substances such as flavonoids, vitamins, terpenes, and lignin have received considerable attention due to their human health benefits and pharmaceutical/medical applications. Among them is lignin, an amorphous biomacromolecule mainly derived from the combinatorial radical coupling of three phenylpropane units (p-hydroxypenyl,...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Fan Shu Bo Jiang Yufeng Yuan Mohan Li Wenjuan Wu Yongcan Jin Huining Xiao Source Type: research

Highly Functional Materials Based on Nano-Lignin, Lignin, and Lignin/Silica Hybrid Capped Silver Nanoparticles with Antibacterial Activities
This study focuses on the sustainably rice-husk-extracted lignin, nano-lignin (n-Lignin), lignin-capped silver nanoparticles (LCSN), n-Lignin-capped silver nanoparticles (n-LCSN), and lignin-capped silica-silver nanoparticles (LCSSN), and using them for antibacterial activities. The final n-Lignin-based products had a sphere-like structure, of which the size varied between 50 and 80 nm. We found that while n-Lignin and lignin were less effective against Escherichia coli than against Staphylococcus aureus, n-Lignin/lignin-based hybrid materials, i.e., n-LCSN, LCSN, and LCSSN, were better against E. coli than against S. aure...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Nhat Thong Tran Trang Thi Thu Nguyen Dat Ha Thu Hien Nguyen Nguyen Ngan Nguyen Kangkyun Baek Ngoc Thuy Nguyen Cong Khanh Tran Thi Thanh Van Tran Hieu Van Le Dang Mao Nguyen DongQuy Hoang Source Type: research

Photoreactive Cytosine-Functionalized Self-Assembled Micelles with Enhanced Cellular Uptake Capability for Efficient Cancer Chemotherapy
Biomacromolecules. 2021 Nov 22. doi: 10.1021/acs.biomac.1c01199. Online ahead of print.ABSTRACTDesign, fabrication, and control of photoreactive supramolecular macromers─which are composed of a thermoresponsive polymer backbone and photoreactive nucleobase end-groups─to achieve the desired physical-chemical performance and provide the high efficiency required for chemotherapy drug delivery purposes still present challenges. Herein, a difunctional cytosine-terminated supramolecular macromer was successfully obtained at high yield. UV-irradiation induces the formation of cytosine photodimers within the structure. The irr...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Abere Habtamu Manayia Fasih Bintang Ilhami Ai-Wei Lee Chih-Chia Cheng Source Type: research

Biological Activities and Emerging Roles of Lignin and Lignin-Based Products ─A Review
Biomacromolecules. 2021 Nov 21. doi: 10.1021/acs.biomac.1c00805. Online ahead of print.ABSTRACTBioactive substances, displaying excellent biocompatibility, chemical stability, and processability, could be extensively applied in biomedicine and tissue engineering. In recent years, plant-based bioactive substances such as flavonoids, vitamins, terpenes, and lignin have received considerable attention due to their human health benefits and pharmaceutical/medical applications. Among them is lignin, an amorphous biomacromolecule mainly derived from the combinatorial radical coupling of three phenylpropane units (p-hydroxypenyl,...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Fan Shu Bo Jiang Yufeng Yuan Mohan Li Wenjuan Wu Yongcan Jin Huining Xiao Source Type: research

Highly Functional Materials Based on Nano-Lignin, Lignin, and Lignin/Silica Hybrid Capped Silver Nanoparticles with Antibacterial Activities
This study focuses on the sustainably rice-husk-extracted lignin, nano-lignin (n-Lignin), lignin-capped silver nanoparticles (LCSN), n-Lignin-capped silver nanoparticles (n-LCSN), and lignin-capped silica-silver nanoparticles (LCSSN), and using them for antibacterial activities. The final n-Lignin-based products had a sphere-like structure, of which the size varied between 50 and 80 nm. We found that while n-Lignin and lignin were less effective against Escherichia coli than against Staphylococcus aureus, n-Lignin/lignin-based hybrid materials, i.e., n-LCSN, LCSN, and LCSSN, were better against E. coli than against S. aure...
Source: Biomacromolecules - November 22, 2021 Category: Biochemistry Authors: Nhat Thong Tran Trang Thi Thu Nguyen Dat Ha Thu Hien Nguyen Nguyen Ngan Nguyen Kangkyun Baek Ngoc Thuy Nguyen Cong Khanh Tran Thi Thanh Van Tran Hieu Van Le Dang Mao Nguyen DongQuy Hoang Source Type: research

Regioselective Protection and Deprotection of Nanocellulose Molecular Design Architecture: Robust Platform for Multifunctional Applications
This study mainly focuses on regioselective installation of trityl protecting groups on nanocellulose chains. The elemental analysis and nuclear magnetic resonance spectroscopy of regioselectively substituted nanofibrillated cellulose (NFC) suggested that the trityl group was successfully grafted in the primary hydroxyl group with a degree of substitution of nearly 1. Hansen solubility parameters were employed, and the binary system composed of an ionic liquid and pyridine as a base was revealed to be the optimum condition for regioselective functionalization of nanocellulose. Interestingly, the dissolution of NFC in the i...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Otavio Augusto Titton Dias Samir Konar Viktoriya Pakharenko Antimo Graziano Alcides Lopes Le ão Jimi Tjong Shaffiq Jaffer Mohini Sain Source Type: research

Composite Microgels for Imaging-Monitored Tracking of the Delivery of Vascular Endothelial Growth Factor to Ischemic Muscles
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01116. Online ahead of print.ABSTRACTMonitoring the supply of vascular endothelial growth factor (VEGF) to ischemic tissues provides information on its biodistribution and delivery to meet the requirements of therapeutic angiogenesis and tissue engineering applications. We herein report the use of microfluidically generated microgels containing VEGF-conjugated fluorescent carbon dots (CDs) (VEGF-CDs), a gelatin-phenol conjugate, and silk fibroin for imaging-monitored tracking of VEGF delivery to ischemic muscles. An in vitro release study and a bioactivity assay ind...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Hamideh Basiri Seyed Sepehr Mohseni Ali Abouei Mehrizi Alireza Rajabnejadkeleshteri Azadeh Ghaee Mehdi Farokhi Eugenia Kumacheva Source Type: research

Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells
In this study, statistical acrylamide-based cationic glycopolymers with glucose or galactose pendant groups were synthesized by reversible addition-fragmentation chain transfer polymerization, and the effects of the saccharide pendant group and cationic monomer loading on polymer cytotoxicity, RNA complexation, and SelK gene knockdown in ISE6 cells were evaluated. All polymers exhibited low cytotoxicity, yet RNA/copolymer complex cell uptake and gene knockdown were highly dependent on the saccharide structure and the N:P (amino to phosphate groups) ratio.PMID:34793129 | DOI:10.1021/acs.biomac.1c00824 (Source: Biomacromolecules)
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Kelli A Stockmal Latoyia P Downs Ashley N Davis Lisa K Kemp Shahid Karim Sarah E Morgan Source Type: research

Reinforcement of Silk Microneedle Patches for Accurate Transdermal Delivery
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01213. Online ahead of print.ABSTRACTMicroneedles (MNs) have attracted considerable attention in the pharmaceutical field as a minimally invasive delivery alternative to hypodermic needles. Current material systems of MNs have gradually shifted from metals, ceramics, and silicon to polymer in consideration of toughness and drug loading capacity. Silk fibroin (SF) is considered one of the most promising alternatives because it combines the ability to maintain the activity of biomolecules, adjustable mechanical strength, and excellent biocompatibility. However, the st...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Zaifu Lin Yanru Li Guoqing Meng Xinling Hu Zhicheng Zeng Bicheng Zhao Naibo Lin Xiang Yang Liu Source Type: research

Redox-Responsive Hydrogels with Decoupled Initial Stiffness and Degradation
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01180. Online ahead of print.ABSTRACTDisulfide-cross-linked hydrogels have been widely used for biological applications because of their degradability in response to redox stimuli. However, degradability often depends on polymer concentration, which also influences the hydrogel mechanical properties such as the initial stiffness. Here, we describe a one-pot cross-linking approach utilizing both a thiol-ene reaction through a Michael pathway with divinyl sulfone (DVS) to form non-reducible thioether bonds and thiol oxidation promoted by ferric ethylenediaminetetraace...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Charng-Yu Lin Carly M Battistoni Julie C Liu Source Type: research

Regioselective Protection and Deprotection of Nanocellulose Molecular Design Architecture: Robust Platform for Multifunctional Applications
This study mainly focuses on regioselective installation of trityl protecting groups on nanocellulose chains. The elemental analysis and nuclear magnetic resonance spectroscopy of regioselectively substituted nanofibrillated cellulose (NFC) suggested that the trityl group was successfully grafted in the primary hydroxyl group with a degree of substitution of nearly 1. Hansen solubility parameters were employed, and the binary system composed of an ionic liquid and pyridine as a base was revealed to be the optimum condition for regioselective functionalization of nanocellulose. Interestingly, the dissolution of NFC in the i...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Otavio Augusto Titton Dias Samir Konar Viktoriya Pakharenko Antimo Graziano Alcides Lopes Le ão Jimi Tjong Shaffiq Jaffer Mohini Sain Source Type: research

Composite Microgels for Imaging-Monitored Tracking of the Delivery of Vascular Endothelial Growth Factor to Ischemic Muscles
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01116. Online ahead of print.ABSTRACTMonitoring the supply of vascular endothelial growth factor (VEGF) to ischemic tissues provides information on its biodistribution and delivery to meet the requirements of therapeutic angiogenesis and tissue engineering applications. We herein report the use of microfluidically generated microgels containing VEGF-conjugated fluorescent carbon dots (CDs) (VEGF-CDs), a gelatin-phenol conjugate, and silk fibroin for imaging-monitored tracking of VEGF delivery to ischemic muscles. An in vitro release study and a bioactivity assay ind...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Hamideh Basiri Seyed Sepehr Mohseni Ali Abouei Mehrizi Alireza Rajabnejadkeleshteri Azadeh Ghaee Mehdi Farokhi Eugenia Kumacheva Source Type: research

Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells
In this study, statistical acrylamide-based cationic glycopolymers with glucose or galactose pendant groups were synthesized by reversible addition-fragmentation chain transfer polymerization, and the effects of the saccharide pendant group and cationic monomer loading on polymer cytotoxicity, RNA complexation, and SelK gene knockdown in ISE6 cells were evaluated. All polymers exhibited low cytotoxicity, yet RNA/copolymer complex cell uptake and gene knockdown were highly dependent on the saccharide structure and the N:P (amino to phosphate groups) ratio.PMID:34793129 | DOI:10.1021/acs.biomac.1c00824 (Source: Biomacromolecules)
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Kelli A Stockmal Latoyia P Downs Ashley N Davis Lisa K Kemp Shahid Karim Sarah E Morgan Source Type: research

Reinforcement of Silk Microneedle Patches for Accurate Transdermal Delivery
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01213. Online ahead of print.ABSTRACTMicroneedles (MNs) have attracted considerable attention in the pharmaceutical field as a minimally invasive delivery alternative to hypodermic needles. Current material systems of MNs have gradually shifted from metals, ceramics, and silicon to polymer in consideration of toughness and drug loading capacity. Silk fibroin (SF) is considered one of the most promising alternatives because it combines the ability to maintain the activity of biomolecules, adjustable mechanical strength, and excellent biocompatibility. However, the st...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Zaifu Lin Yanru Li Guoqing Meng Xinling Hu Zhicheng Zeng Bicheng Zhao Naibo Lin Xiang Yang Liu Source Type: research

Redox-Responsive Hydrogels with Decoupled Initial Stiffness and Degradation
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01180. Online ahead of print.ABSTRACTDisulfide-cross-linked hydrogels have been widely used for biological applications because of their degradability in response to redox stimuli. However, degradability often depends on polymer concentration, which also influences the hydrogel mechanical properties such as the initial stiffness. Here, we describe a one-pot cross-linking approach utilizing both a thiol-ene reaction through a Michael pathway with divinyl sulfone (DVS) to form non-reducible thioether bonds and thiol oxidation promoted by ferric ethylenediaminetetraace...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Charng-Yu Lin Carly M Battistoni Julie C Liu Source Type: research

Regioselective Protection and Deprotection of Nanocellulose Molecular Design Architecture: Robust Platform for Multifunctional Applications
This study mainly focuses on regioselective installation of trityl protecting groups on nanocellulose chains. The elemental analysis and nuclear magnetic resonance spectroscopy of regioselectively substituted nanofibrillated cellulose (NFC) suggested that the trityl group was successfully grafted in the primary hydroxyl group with a degree of substitution of nearly 1. Hansen solubility parameters were employed, and the binary system composed of an ionic liquid and pyridine as a base was revealed to be the optimum condition for regioselective functionalization of nanocellulose. Interestingly, the dissolution of NFC in the i...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Otavio Augusto Titton Dias Samir Konar Viktoriya Pakharenko Antimo Graziano Alcides Lopes Le ão Jimi Tjong Shaffiq Jaffer Mohini Sain Source Type: research

Composite Microgels for Imaging-Monitored Tracking of the Delivery of Vascular Endothelial Growth Factor to Ischemic Muscles
Biomacromolecules. 2021 Nov 18. doi: 10.1021/acs.biomac.1c01116. Online ahead of print.ABSTRACTMonitoring the supply of vascular endothelial growth factor (VEGF) to ischemic tissues provides information on its biodistribution and delivery to meet the requirements of therapeutic angiogenesis and tissue engineering applications. We herein report the use of microfluidically generated microgels containing VEGF-conjugated fluorescent carbon dots (CDs) (VEGF-CDs), a gelatin-phenol conjugate, and silk fibroin for imaging-monitored tracking of VEGF delivery to ischemic muscles. An in vitro release study and a bioactivity assay ind...
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Hamideh Basiri Seyed Sepehr Mohseni Ali Abouei Mehrizi Alireza Rajabnejadkeleshteri Azadeh Ghaee Mehdi Farokhi Eugenia Kumacheva Source Type: research

Cationic Glycopolyelectrolytes for RNA Interference in Tick Cells
In this study, statistical acrylamide-based cationic glycopolymers with glucose or galactose pendant groups were synthesized by reversible addition-fragmentation chain transfer polymerization, and the effects of the saccharide pendant group and cationic monomer loading on polymer cytotoxicity, RNA complexation, and SelK gene knockdown in ISE6 cells were evaluated. All polymers exhibited low cytotoxicity, yet RNA/copolymer complex cell uptake and gene knockdown were highly dependent on the saccharide structure and the N:P (amino to phosphate groups) ratio.PMID:34793129 | DOI:10.1021/acs.biomac.1c00824 (Source: Biomacromolecules)
Source: Biomacromolecules - November 18, 2021 Category: Biochemistry Authors: Kelli A Stockmal Latoyia P Downs Ashley N Davis Lisa K Kemp Shahid Karim Sarah E Morgan Source Type: research