Supramolecular Organo/hydrogel-Fabricated Long Alkyl Chain α-Amidoamides as a Smart Soft Material for pH-Responsive Curcumin Release
In this study, we have designed and synthesized a series of six α-amidoamides by varying the hydrophobic alkyl chain length (C12-C22), which were well characterized using different spectral techniques. These α-amidoamides formed self-assembled aggregates in a DMSO/water solvent system affording organo/hydrogels at 0.66% w/v, which is the minimum gelation concentration (MGC) making them as remarkable supergelators. The various functionalities present in these gelators such as amides and alkyl chain length pave the way toward excellent gelation mechanism through hydrogen bonding and van der Waals interaction as evidenced f...
Source: Biomacromolecules - January 8, 2024 Category: Biochemistry Authors: Sharol Sebastian Yajat Rohila Eqvinshi Yadav Priya Bhardwaj Yangala Sudheer Babu Mulaka Maruthi Azaj Ansari Manoj K Gupta Source Type: research
Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles
Biomacromolecules. 2024 Jan 8. doi: 10.1021/acs.biomac.3c00972. Online ahead of print.ABSTRACTConsiderable attention has been dedicated to lipid rafts due to their importance in numerous cell functions such as membrane trafficking, polarization, and signaling. Next to studies in living cells, artificial micrometer-sized vesicles with a minimal set of components are established as a major tool to understand the phase separation dynamics and their intimate interplay with membrane proteins. In parallel, mixtures of phospholipids and certain amphiphilic polymers simultaneously offer an interface for proteins and mimic this seg...
Source: Biomacromolecules - January 8, 2024 Category: Biochemistry Authors: Nika Otrin Lado Otrin Claudia Bednarz Toni K Tr äger Farzad Hamdi Panagiotis L Kastritis Ivan Ivanov Kai Sundmacher Source Type: research
Schiff-Base Cross-Linked Poly(2-oxazoline) Micelle Drug Conjugates Possess Antiferroptosis Activity in Numerous In Vitro Cell Models
Biomacromolecules. 2024 Jan 4. doi: 10.1021/acs.biomac.3c01106. Online ahead of print.ABSTRACTA great deal of nanocarriers have been applied to induce ferroptosis in cancer research, yet there are limited examples of nanocarrier formulations to rescue ferroptosis, which can be applied to neurodegeneration, inflammation, liver damage, kidney disease, and more. Here, we present the synthesis, characterization, and in vitro evaluation of pH-responsive, core-cross-linked micelle (CCM) ferrostatin-1 (Fer-1) conjugates with amine, valproic acid, and biotin surface chemistries. Fer-1 release from stable and defined CCM Fer-1 conj...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Joshua P Morrow Zihnil A I Mazrad Nicole M Warne Scott Ayton Ashley I Bush Kristian Kempe Source Type: research
Polyamidoamine-Carbon Nanodot Conjugates with Bioreducible Building Blocks: Smart Theranostic Platforms for Targeted siRNA Delivery
This study focuses on designing hybrid theranostic nanosystems, utilizing gadolinium-doped carbon nanodots decorated with bioreducible amphoteric polyamidoamines (PAAs). The objective is to synergize the exceptional theranostic properties of gadolinium-doped carbon nanodots (CDs) with the siRNA complexation capabilities of PAAs. Linear copolymeric polyamidoamines, based on N,N'-bis(acryloyl)cystamine, arginine, and agmatine, were synthesized, resulting in three distinct amphoteric copolymers. Notably, sulfur bridges within the PAA repeating units confer pronounced susceptibility to glutathione-mediated degradation─a key ...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Salvatore Emanuele Drago Mara Andrea Utzeri Nicol ò Mauro Gennara Cavallaro Source Type: research
Stiffness and BMP-2 Mimetic Peptide Jointly Regulate the Osteogenic Differentiation of Rat Bone Marrow Stromal Cells in a Gelatin Cryogel
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01045. Online ahead of print.ABSTRACTBoth biochemical and mechanical cues could regulate the function of stem cells, but the interaction mechanism of their signaling pathway remains unclear, especially in the three-dimensional (3D) culture mode. Higher matrix stiffness promotes osteogenic differentiation of stem cells, and bone morphogenic protein-2 (BMP-2) has been clinically applied to promote bone regeneration. Here, the crosstalk of extracellular mechanical signals on BMP-2 signaling was investigated in rat bone marrow stromal cells (rMSCs) cultured inside cryoge...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Sijing Li Shixiong Zhang Shuao Dong Mengen Zhao Wei Zhang Chao Zhang Zhaoying Wu Source Type: research
Electrostatically Mediated In Situ Polymerization for Enzyme Immobilization and Activation
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c00993. Online ahead of print.ABSTRACTEnzyme immobilization in nanoparticles is of interest for boosting their catalytic applications, yet rational approaches to designs achieving both high enzyme loading and activation remain a challenge. Herein, we report an electrostatically mediated in situ polymerization strategy that simultaneously realizes enzyme immobilization and activation. This was achieved by copolymerizing cationic monomers with a cross-linker in the presence of the enzyme lipase (anionic) as the template, which produces enzyme-loaded nanogels. The effect...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Yuting Wan Jin Zhou Jiaying Ni Ying Cai Martien Cohen Stuart Junyou Wang Source Type: research
Unsymmetrical Low-Generation Cationic Phosphorus Dendrimers as a Nonviral Vector to Deliver MicroRNA for Breast Cancer Therapy
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01169. Online ahead of print.ABSTRACTThe development of nonviral dendritic polymers with a simple molecular backbone and great gene delivery efficiency to effectively tackle cancer remains a great challenge. Phosphorus dendrimers or dendrons are promising vectors due to their structural uniformity, rigid molecular backbones, and tunable surface functionalities. Here, we report the development of a new low-generation unsymmetrical cationic phosphorus dendrimer bearing 5 pyrrolidinium groups and one amino group as a nonviral gene delivery vector. The created AB5-type d...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Yu Zou Siyan Shen Andrii Karpus Huxiao Sun Regis Laurent Anne-Marie Caminade Mingwu Shen Serge Mignani Xiangyang Shi Jean-Pierre Majoral Source Type: research
Ionizable Polymeric Micelles with Phenylalanine Moieties Enhance Intracellular Delivery of Self-Replicating RNA for Long-Lasting Protein Expression In Vivo
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01102. Online ahead of print.ABSTRACTmRNA-based therapeutics are revolutionizing the landscape of medical interventions. However, the short half-life of mRNA and transient protein expression often limits its therapeutic potential, demanding high treatment doses or repeated administrations. Self-replicating RNA (RepRNA)-based treatments could offer enhanced protein production and reduce the required dosage. Here, we developed polymeric micelles based on flexible poly(ethylene glycol)-poly(glycerol) (PEG-PG) block copolymers modified with phenylalanine (Phe) moieties v...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Lucas Mixich Eger Boonstra Keita Masuda Shang-Wei Li Yuki Nakashima Fanlu Meng Momoko Sakata Tatsuro Goda Satoshi Uchida Horacio Cabral Source Type: research
Schiff-Base Cross-Linked Poly(2-oxazoline) Micelle Drug Conjugates Possess Antiferroptosis Activity in Numerous In Vitro Cell Models
Biomacromolecules. 2024 Jan 4. doi: 10.1021/acs.biomac.3c01106. Online ahead of print.ABSTRACTA great deal of nanocarriers have been applied to induce ferroptosis in cancer research, yet there are limited examples of nanocarrier formulations to rescue ferroptosis, which can be applied to neurodegeneration, inflammation, liver damage, kidney disease, and more. Here, we present the synthesis, characterization, and in vitro evaluation of pH-responsive, core-cross-linked micelle (CCM) ferrostatin-1 (Fer-1) conjugates with amine, valproic acid, and biotin surface chemistries. Fer-1 release from stable and defined CCM Fer-1 conj...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Joshua P Morrow Zihnil A I Mazrad Nicole M Warne Scott Ayton Ashley I Bush Kristian Kempe Source Type: research
Polyamidoamine-Carbon Nanodot Conjugates with Bioreducible Building Blocks: Smart Theranostic Platforms for Targeted siRNA Delivery
This study focuses on designing hybrid theranostic nanosystems, utilizing gadolinium-doped carbon nanodots decorated with bioreducible amphoteric polyamidoamines (PAAs). The objective is to synergize the exceptional theranostic properties of gadolinium-doped carbon nanodots (CDs) with the siRNA complexation capabilities of PAAs. Linear copolymeric polyamidoamines, based on N,N'-bis(acryloyl)cystamine, arginine, and agmatine, were synthesized, resulting in three distinct amphoteric copolymers. Notably, sulfur bridges within the PAA repeating units confer pronounced susceptibility to glutathione-mediated degradation─a key ...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Salvatore Emanuele Drago Mara Andrea Utzeri Nicol ò Mauro Gennara Cavallaro Source Type: research
Stiffness and BMP-2 Mimetic Peptide Jointly Regulate the Osteogenic Differentiation of Rat Bone Marrow Stromal Cells in a Gelatin Cryogel
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01045. Online ahead of print.ABSTRACTBoth biochemical and mechanical cues could regulate the function of stem cells, but the interaction mechanism of their signaling pathway remains unclear, especially in the three-dimensional (3D) culture mode. Higher matrix stiffness promotes osteogenic differentiation of stem cells, and bone morphogenic protein-2 (BMP-2) has been clinically applied to promote bone regeneration. Here, the crosstalk of extracellular mechanical signals on BMP-2 signaling was investigated in rat bone marrow stromal cells (rMSCs) cultured inside cryoge...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Sijing Li Shixiong Zhang Shuao Dong Mengen Zhao Wei Zhang Chao Zhang Zhaoying Wu Source Type: research
Electrostatically Mediated In Situ Polymerization for Enzyme Immobilization and Activation
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c00993. Online ahead of print.ABSTRACTEnzyme immobilization in nanoparticles is of interest for boosting their catalytic applications, yet rational approaches to designs achieving both high enzyme loading and activation remain a challenge. Herein, we report an electrostatically mediated in situ polymerization strategy that simultaneously realizes enzyme immobilization and activation. This was achieved by copolymerizing cationic monomers with a cross-linker in the presence of the enzyme lipase (anionic) as the template, which produces enzyme-loaded nanogels. The effect...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Yuting Wan Jin Zhou Jiaying Ni Ying Cai Martien Cohen Stuart Junyou Wang Source Type: research
Unsymmetrical Low-Generation Cationic Phosphorus Dendrimers as a Nonviral Vector to Deliver MicroRNA for Breast Cancer Therapy
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01169. Online ahead of print.ABSTRACTThe development of nonviral dendritic polymers with a simple molecular backbone and great gene delivery efficiency to effectively tackle cancer remains a great challenge. Phosphorus dendrimers or dendrons are promising vectors due to their structural uniformity, rigid molecular backbones, and tunable surface functionalities. Here, we report the development of a new low-generation unsymmetrical cationic phosphorus dendrimer bearing 5 pyrrolidinium groups and one amino group as a nonviral gene delivery vector. The created AB5-type d...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Yu Zou Siyan Shen Andrii Karpus Huxiao Sun Regis Laurent Anne-Marie Caminade Mingwu Shen Serge Mignani Xiangyang Shi Jean-Pierre Majoral Source Type: research
Ionizable Polymeric Micelles with Phenylalanine Moieties Enhance Intracellular Delivery of Self-Replicating RNA for Long-Lasting Protein Expression In Vivo
Biomacromolecules. 2024 Jan 5. doi: 10.1021/acs.biomac.3c01102. Online ahead of print.ABSTRACTmRNA-based therapeutics are revolutionizing the landscape of medical interventions. However, the short half-life of mRNA and transient protein expression often limits its therapeutic potential, demanding high treatment doses or repeated administrations. Self-replicating RNA (RepRNA)-based treatments could offer enhanced protein production and reduce the required dosage. Here, we developed polymeric micelles based on flexible poly(ethylene glycol)-poly(glycerol) (PEG-PG) block copolymers modified with phenylalanine (Phe) moieties v...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Lucas Mixich Eger Boonstra Keita Masuda Shang-Wei Li Yuki Nakashima Fanlu Meng Momoko Sakata Tatsuro Goda Satoshi Uchida Horacio Cabral Source Type: research
Schiff-Base Cross-Linked Poly(2-oxazoline) Micelle Drug Conjugates Possess Antiferroptosis Activity in Numerous In Vitro Cell Models
Biomacromolecules. 2024 Jan 4. doi: 10.1021/acs.biomac.3c01106. Online ahead of print.ABSTRACTA great deal of nanocarriers have been applied to induce ferroptosis in cancer research, yet there are limited examples of nanocarrier formulations to rescue ferroptosis, which can be applied to neurodegeneration, inflammation, liver damage, kidney disease, and more. Here, we present the synthesis, characterization, and in vitro evaluation of pH-responsive, core-cross-linked micelle (CCM) ferrostatin-1 (Fer-1) conjugates with amine, valproic acid, and biotin surface chemistries. Fer-1 release from stable and defined CCM Fer-1 conj...
Source: Biomacromolecules - January 5, 2024 Category: Biochemistry Authors: Joshua P Morrow Zihnil A I Mazrad Nicole M Warne Scott Ayton Ashley I Bush Kristian Kempe Source Type: research
