Beyond the Surface: A Methodological Exploration of Enzyme Impact along the Cellulose Fiber Cross-Section

Biomacromolecules. 2024 Apr 18. doi: 10.1021/acs.biomac.4c00152. Online ahead of print.ABSTRACTDespite the wide range of analytical tools available for the characterization of cellulose, the in-depth characterization of inhomogeneous, layered cellulose fiber structures remains a challenge. When treating fibers or spinning man-made fibers, the question always arises as to whether the changes in the fiber structure affect only the surface or the entire fiber. Here, we developed an analysis tool based on the sequential limited dissolution of cellulose fiber layers. The method can reveal potential differences in fiber properties along the cross-sectional profile of natural or man-made cellulose fibers. In this analytical approach, carbonyl groups are labeled with a carbonyl selective fluorescence label (CCOA), after which thin fiber layers are sequentially dissolved with the solvent system DMAc/LiCl (9% w/v) and analyzed with size exclusion chromatography coupled with light scattering and fluorescence detection. The analysis of these fractions allowed for the recording of the changes in the chemical structure across the layers, resulting in a detailed cross-sectional profile of the different functionalities and molecular weight distributions. The method was optimized and tested in practice with LPMO (lytic polysaccharide monooxygenase)-treated cotton fibers, where it revealed the depth of fiber modification by the enzyme.PMID:38634234 | DOI:10.1021/acs.biomac.4c00152

Source: Biomacromolecules - April 18, 2024 Category: Biochemistry Authors: Irina Sulaeva Fredrik Gjerstad Sto Pamo Ivan Melikhov David Budischowsky Jenni L Rahikainen Anna Borisova Kaisa Marjamaa Kristiina Kruus Vincent G H Eijsink Anik ó Várnai Antje Potthast Source Type: research