Molecules, Vol. 25, Pages 3134: Chiral Separation and Determination of Etoxazole Enantiomers in Vegetables by Normal-Phase and Reverse-Phase High Performance Liquid Chromatography
o Wang Lin He The chiral separation of etoxazole enantiomers on Lux Cellulose-1, Lux Cellulose-3, Chiralpak IC, and Chiralpak AD chiral columns was carefully investigated by normal-phase high performance liquid chromatography and reverse-phase high performance liquid chromatography (HPLC). Hexane/isopropanol, hexane/n-butanol, methanol/water, and acetonitrile/water were used as mobile phase at a flow rate of 0.8 mL/min. The effects of chiral stationary phase, mobile phase component, mobile phase ratio, and temperature on etoxazole separation were also studied. Etoxazole enantiomers were baseline separated on Lux Cell...
Source: Molecules - July 9, 2020 Category: Chemistry Authors: Ping Zhang Yuhan He Sheng Wang Dongmei Shi Yangyang Xu Furong Yang Jianhao Wang Lin He Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3135: New Dihydroxytyrosyl Esters from Dicarboxylic Acids: Synthesis and Evaluation of the Antioxidant Activity In Vitro (ABTS) and in Cell-Cultures (DCF Assay)
Daniela Tofani New dihydroxytyrosyl esters 2a, 2c–2j of dicarboxylic acids were synthesized from methyl orthoformate protected hydroxytyrosol 3 and diacyl chlorides. New compounds were characterized (HRMS, FT-IR, 1H- and 13C-NMR), and tested for antioxidant activity both in vitro (ABTS) and on L6 myoblasts and THP1 leukemic monocytes cell culture by DCF assay. According to the ABTS assay, compounds 2a, 2c–2j showed a TEAC value of antioxidant capacity up to twice that of Trolox. Very high or complete ROS protections were obtained in the cell environment where lipophilicity and rigidity of d...
Source: Molecules - July 9, 2020 Category: Chemistry Authors: Elia Roma Elena Mattoni Paolo Lupattelli Seyed Sepehr Moeini Tecla Gasperi Roberta Bernini Sandra Incerpi Daniela Tofani Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3136: Metabolic Engineering Escherichia coli for the Production of Lycopene
ng Yang Lycopene, a potent antioxidant, has been widely used in the fields of pharmaceuticals, nutraceuticals, and cosmetics. However, the production of lycopene extracted from natural sources is far from meeting the demand. Consequently, synthetic biology and metabolic engineering have been employed to develop microbial cell factories for lycopene production. Due to the advantages of rapid growth, complete genetic background, and a reliable genetic operation technique, Escherichia coli has become the preferred host cell for microbial biochemicals production. In this review, the recent advances in biological lycopene p...
Source: Molecules - July 9, 2020 Category: Chemistry Authors: Zhaobao Wang JingXin Sun Qun Yang Jianming Yang Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3137: Synthesis and Immunological Evaluation of a Single Molecular Construct MUC1 Vaccine Containing l-Rhamnose Repeating Units
A. Wall A rhamnose targeting strategy for generating effective anticancer vaccines was successful in our previous studies. We showed that by utilizing natural anti-rhamnose antibodies, a rhamnose-containing vaccine can be targeted to antigen-presenting cells, such as dendritic cells. In this case, rhamnose (Rha) was linked directly to the liposomes bearing the antigen. However, in the current approach, we conjugated a multivalent Tri-Rha ligand with the antigen itself, making it a single component vaccine construct, unlike the previous two-component vaccine construct where Rha cholesterol and Mucin1 (MUC1) antigen were...
Source: Molecules - July 9, 2020 Category: Chemistry Authors: Md Kamal Hossain Abhishek Vartak Steven J. Sucheck Katherine A. Wall Tags: Communication Source Type: research

Molecules, Vol. 25, Pages 3106: Increased Adsorption of Heavy Metal Ions in Multi-Walled Carbon Nanotubes with Improved Dispersion Stability
In this study, we test the effect of dispersion of oxidized multi-walled carbon nanotubes (MWCNTs) on the removal of heavy metals from acidic solutions. For this, polyurethane filters were dyed with a well-dispersed oxidized MWCNTs solution using chemical and mechanical dispersion methods. Filters were used in column experiments, and the sorption capacity increased more than six times (600%) compared to experiments with suspended MWCNTs. Further, kinetic experiments showed a faster saturation on MWCNTs in column experiments. These results contribute to a better understanding of the effect of dispersion on the use of CNTs a...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Carolina Rodr íguez Sebasti án Briano Eduardo Leiva Tags: Communication Source Type: research

Molecules, Vol. 25, Pages 3105: Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta
Bravo Fredi Cifuentes Heliotropium taltalense is an endemic species of the northern coast of Chile and is used as folk medicine. The polyphenolic composition of the methanolic and aqueous extract of the endemic Chilean species was investigated using Ultrahigh-Performance Liquid Chromatography, Heated Electrospray Ionization and Mass Spectrometry (UHPLC-Orbitrap-HESI-MS). Fifty-three compounds were detected, mainly derivatives of benzoic acid, flavonoids, and some phenolic acids. Furthermore, five major compounds were isolated by column chromatography from the extract, including four flavonoids and one geranyl benzoic...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Ruth E. Barrientos Mario J. Simirgiotis Javier Palacios Adri án Paredes Jorge B órquez Alejandra Bravo Fredi Cifuentes Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3107: X6: A Novel Antibody for Potential Use in Gluten Quantification
This study investigates the characteristics of a novel anti-gliadin antibody X6. We found the QXQPFPXP site to be a recognized epitope that provides specific binding of the antibody to cereal prolamins involved in celiac disease manifestation. A specificity study using immunoblotting shows the recognition of wheat, barley and rye proteins—as well as α-gliadin homologs from non-edible cereals (Dasypyrum villosum). Reactivity to avenin was less pronounced, as this protein does not contain the PFP motif most critical for antibody recognition. The proteins of Zea mays and Setaria italica were not re...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Aleksandrina Shatalova Ivan Shatalov Yuri Lebedin Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3108: Synthetic Mono-Rhamnolipids Display Direct Antifungal Effects and Trigger an Innate Immune Response in Tomato against Botrytis Cinerea
Stephan Dorey Essaid Ait Barka Natural rhamnolipids are potential biocontrol agents for plant protection against bacterial and fungal diseases. In this work, we synthetized new synthetic mono-rhamnolipids (smRLs) consisting in a rhamnose connected to a simple acyl chain and differing by the nature of the link and the length of the lipid tail. We then investigated the effects of these ether, ester, carbamate or succinate smRL derivatives on Botrytis cinerea development, symptoms spreading on tomato leaves and immune responses in tomato plants. Our results demonstrate that synthetic smRLs are able to trigger early and...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Mathilde Robineau Sarah Le Guenic Lisa Sanchez Ludovic Chaveriat Vincent Lequart Nicolas Joly Maryline Calonne C édric Jacquard St éphane Declerck Patrick Martin Stephan Dorey Essaid Ait Barka Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3110: Interaction of Ampicillin and Amoxicillin with Mn2+: A Speciation Study in Aqueous Solution
rè A potentiometric and UV spectrophotometric investigation on Mn2+-ampicillin and Mn2+-amoxicillin systems in NaCl aqueous solution is reported. The potentiometric measurements were carried out under different conditions of temperature (15 ≤ t/°C ≤ 37). The obtained speciation pattern includes two species for both the investigated systems. More in detail, for system containing ampicillin MLH and ML species, for that containing amoxicillin, MLH2 and MLH ones. The spectrophotometric findings have fully confirmed the results obtained by potentiometry for both the systems, in terms...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Foti Giuffr è Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3111: Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1
Lazari Bilia Essential oils are complex mixtures of strongly active compounds, very volatile and sensitive to light, oxygen, moisture and temperature. Loading inside nanocarriers can be a strategy to increase their stability and successfully use them in therapy. In the present study, a commercial Melissa officinalis L. (Lamiaceae) essential oil (MEO) was analyzed by gas chromatography-mass spectrometry, loaded inside glycerosomes (MEO-GS) and evaluated for its anti-herpetic activity against HSV type 1. MEO-GS analyses were prepared by the thin layer evaporation method and they were characterized by light scattering...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Vanti Ntallis Panagiotidis Dourdouni Patsoura Bergonzi Lazari Bilia Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3109: Optimisation of Sporosori Purification and Protein Extraction Techniques for the Biotrophic Protozoan Plant Pathogen Spongospora subterranea
R. Wilson Spongospora subterranea is a soil-borne plant pathogen responsible for the economically significant root and powdery scab diseases of potato. However, the obligate biotrophic nature of S. subterranea has made the detailed study of the pathogen problematic. Here, we first compared the benefits of sporosori partial purification utilizing Ludox® gradient centrifugation. We then undertook optimization efforts for protein isolation comparing the use of a urea buffer followed by single-pot solid-phase-enhanced sample preparation (SP3) and a sodium dodecyl sulphate (SDS) buffer followed by suspension-tra...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Sadegh Balotf Richard Wilson Robert S. Tegg David S. Nichols Calum R. Wilson Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3113: Systematics of Atomic Orbital Hybridization of Coordination Polyhedra: Role of f Orbitals
ng The combination of atomic orbitals to form hybrid orbitals of special symmetries can be related to the individual orbital polynomials. Using this approach, 8-orbital cubic hybridization can be shown to be sp3d3f requiring an f orbital, and 12-orbital hexagonal prismatic hybridization can be shown to be sp3d5f2g requiring a g orbital. The twists to convert a cube to a square antiprism and a hexagonal prism to a hexagonal antiprism eliminate the need for the highest nodality orbitals in the resulting hybrids. A trigonal twist of an Oh octahedron into a D3h trigonal prism can involve a gradual change of the pair of d o...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: King Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3112: Taste Processing: Insights from Animal Models
a-Urbina Taste processing is an adaptive mechanism involving complex physiological, motivational and cognitive processes. Animal models have provided relevant data about the neuroanatomical and neurobiological components of taste processing. From these models, two important domains of taste responses are described in this review. The first part focuses on the neuroanatomical and neurophysiological bases of olfactory and taste processing. The second part describes the biological and behavioral characteristics of taste learning, with an emphasis on conditioned taste aversion as a key process for the survival and health o...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Andr és Molero-Chamizo Guadalupe Nathzidy Rivera-Urbina Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3114: Method Validation and Investigation of the Levels of Pharmaceuticals and Personal Care Products in Sludge of Wastewater Treatment Plants and Soils of Irrigated Golf Course
In this study, the variation of the sonication temperature, type of organic solvent for extraction, and types of cartridge were used to optimize the extraction procedure. A good repeatability (within day) and reproducibility (between days) with a relative standard deviation (RSD) that was equal or less than 13% for all the PPCPs were achieved with the developed extraction procedures for the irrigated soil and sewage sludge samples. The limits of detection (LODs) of the tested compounds varied from 0.1 ng·g−1 (aspirin) to 1.4 ng·g−1 (doxycycline) and from 0.1 ng&...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Olufemi Temitope Ademoyegun Omobola Oluranti Okoh Anthony Ifeanyi Okoh Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3115: Structure-Based Design of Biologically Active Compounds
a The past decades have witnessed tremendous progress in the detailed structural knowledge of proteins as potential or validated drug targets and the discovery of new drugs based on this wealth of knowledge progressed in parallel [...] (Source: Molecules)
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Sandra Gemma Tags: Editorial Source Type: research

Molecules, Vol. 25, Pages 3118: 5-Aryl-2-(3,5-dialkyl-4-hydroxyphenyl)-4,4-dimethyl-4H-imidazole 3-Oxides and Their Redox Species: How Antioxidant Activity of 1-Hydroxy-2,5-dihydro-1H-imidazoles Correlates with the Stability of Hybrid Phenoxyl –Nitroxides
Molecules, Vol. 25, Pages 3118: 5-Aryl-2-(3,5-dialkyl-4-hydroxyphenyl)-4,4-dimethyl-4H-imidazole 3-Oxides and Their Redox Species: How Antioxidant Activity of 1-Hydroxy-2,5-dihydro-1H-imidazoles Correlates with the Stability of Hybrid Phenoxyl–Nitroxides Molecules doi: 10.3390/molecules25143118 Authors: Svetlana A. Amitina Elena V. Zaytseva Natalya A. Dmitrieva Alyona V. Lomanovich Natalya V. Kandalintseva Yury A. Ten Ilya A. Artamonov Alexander F. Markov Dmitrii G. Mazhukin Cyclic nitrones of the imidazole series, containing a sterically hindered phenol group, are promising objects for studyi...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Svetlana A. Amitina Elena V. Zaytseva Natalya A. Dmitrieva Alyona V. Lomanovich Natalya V. Kandalintseva Yury A. Ten Ilya A. Artamonov Alexander F. Markov Dmitrii G. Mazhukin Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3116: Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich Premna odorata Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver
elmohsen Hassan Premna odorata Blanco (Lamiaceae) is an ethnomedicinal plant native to different tropical regions. Although some reports addressed their anti-inflammatory, cytotoxic, and antituberculotic effects, their hepatoprotective potential is yet to be discovered. Accordingly, this study investigated the crude extract and different fractions of the plant leaves; metabolic profiling using liquid chromatography / high-resolution electrospray ionization mass spectroscopy (LC–HRESIMS) analysis, in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties for the dereplic...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Elmaidomy Alhadrami Amin Aly Othman Rateb Hetta Abdelmohsen Hassan Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3120: Biological Activities of Aqueous Extracts from Carob Plant (Ceratonia siliqua L.) by Antioxidant, Analgesic and Proapoptotic Properties Evaluation
n D. Muller The present work describes the volatile compounds profile and phytochemical content of Ceratonia siliqua L. Fifty different components have been identified. Among them, three constituents are shared i.e., 2-methlybutanoic acid, methyl hexanoate and limonene by different common carob preparations: pulp decoction (PD), seeds decoction (SD) and Rob, a sweet syrup extracted from the pulp of the carob pod. Each extract exhibits different volatile aromatic emission profiles. The antioxidant activity of the extracts was evaluated using three methods, DPPH, ABTS and FRAP, producing a dose-dependent response. The IC...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Siwar Ben Ayache Emna Behija Saafi Fathi Emhemmed Guido Flamini Lotfi Achour Christian D. Muller Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3119: Self-Nanoemulsifying Drug Delivery Systems for Enhancing Solubility, Permeability, and Bioavailability of Sesamin
-Tse Wu Sesamin (SSM) is a water-insoluble compound that is easily eliminated by liver metabolism. To improve the solubility and bioavailability of SSM, this study developed and characterized a self-nanoemulsifying drug delivery system (SNEDDS) for the oral delivery of SSM and conducted pharmacokinetic assessments. Oil and surfactant materials suitable for SNEDDS preparation were selected on the basis of their saturation solubility at 37 ± 0.5 °C. The mixing ratios of excipients were determined on the basis of their dispersibility, transmittance (%), droplet sizes, and polydispersity index. A...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Chih-Yuan Wang Ching-Chi Yen Mei-Chich Hsu Yu-Tse Wu Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3117: Ameliorated Mechanical and Dielectric Properties of Heat-Resistant Radome Cyanate Composites
eng Sun In order to improve the mechanical and dielectric properties of radome cyanate, a synergistic reinforcement method is employed to develop a resin-based ternary-composite with high heat-resistance and preferable radar-band transmission, which is expected to be applied to fabricate radomes capable of resisting high temperature and strong electric field. According to copolymerization characteristics and self-curing mechanism, epoxy resin (EP) and bismaleimide (BMI) are employed as reinforcements mixed into a cyanate ester (CE) matrix to prepare CE/BMI/EP composites of a heat-resistant radome material by high-tempe...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Hou-Yu Li Chang-Ming Li Jun-Guo Gao Wei-Feng Sun Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3123: Sustainable Carbon as Efficient Support for Metal-Based Nanocatalyst: Applications in Energy Harvesting and Storage
o Quijano-López Sustainable activated carbon can be obtained from the pyrolysis/activation of biomass wastes coming from different origins. Carbon obtained in this way shows interesting properties, such as high surface area, electrical conductivity, thermal and chemical stability, and porosity. These characteristics among others, such as a tailored pore size distribution and the possibility of functionalization, lead to an increased use of activated carbons in catalysis. The use of activated carbons from biomass origins is a step forward in the development of more sustainable processes enhancing material recycli...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Mireia Buaki-Sog ó Leire Zubizarreta Marta Garc ía-Pellicer Alfredo Quijano-L ópez Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3121: Covalently Functionalized DNA Duplexes and Quadruplexes as Hybrid Catalysts in an Enantioselective Friedel –Crafts Reaction
This study unveils a new and simple way to control the stereochemical outcome of a Friedel–Crafts reaction. (Source: Molecules)
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Surjendu Dey Andres J äschke Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3124: 7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by I κBα Degradation and MAPK Activation in Macrophage Cells
In conclusion, we generated an anti-inflammatory compound through acetylation and demonstrated its efficacy in cell-based in vitro assays. (Source: Molecules)
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Taejin Park Jin-Soo Park Ji Han Sim Seung-Young Kim Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3125: In Vitro Antimycobacterial Activity and Physicochemical Characterization of Diaryl Ether Triclosan Analogues as Potential InhA Reductase Inhibitors
Panda Amany AL-Mahmoudy Two sets of diphenyl ether derivatives incorporating five-membered 1,3,4-oxadiazoles, and their open-chain aryl hydrazone analogs were synthesized in good yields. Most of the synthesized compounds showed promising in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv. Three diphenyl ether derivatives, namely hydrazide 3, oxadiazole 4 and naphthylarylidene 8g exhibited pronounced activity with minimum inhibitory concentrations (MICs) of 0.61, 0.86 and 0.99 μg/mL, respectively compared to triclosan (10 μg/mL) and isoniazid (INH) (0.2 μg/mL). Compounds 3, 4, and 8g...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Tarek Ibrahim Ehab Taher Ebtihal Samir Azizah Malebari Ahdab Khayyat Mamdouh Mohamed Riham Bokhtia Mohammed AlAwadh Israa Seliem Hani Asfour Nabil Alhakamy Siva Panda Amany AL-Mahmoudy Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3122: Properly Substituted Cyclic Bis-(2-bromobenzylidene) Compounds Behaved as Dual p300/CARM1 Inhibitors and Induced Apoptosis in Cancer Cells
lente Antonello Mai Bis-(3-bromo-4-hydroxy)benzylidene cyclic compounds have been reported by us as epigenetic multiple ligands, but different substitutions at the two wings provided analogues with selective inhibition. Since the 1-benzyl-3,5-bis((E)-3-bromobenzylidene)piperidin-4-one 3 displayed dual p300/EZH2 inhibition joined to cancer-selective cell death in a panel of tumor cells and in in vivo xenograft models, we prepared a series of bis((E)-2-bromobenzylidene) cyclic compounds 4a–n to test in biochemical (p300, PCAF, SIRT1/2, EZH2, and CARM1) and cellular (NB4, U937, MCF-7, SH-SY5Y) assays. The ...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Rossella Fioravanti Stefano Tomassi Elisabetta Di Bello Annalisa Romanelli Andrea Maria Plateroti Rosaria Benedetti Mariarosaria Conte Ettore Novellino Lucia Altucci Sergio Valente Antonello Mai Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3126: The Influence of Single, Tandem, and Clustered DNA Damage on the Electronic Properties of the Double Helix: A Theoretical Study
Oxidatively generated damage to DNA frequently appears in the human genome as the effect of aerobic metabolism or as the result of exposure to exogenous oxidizing agents, such as ionization radiation. In this paper, the electronic properties of single, tandem, and clustered DNA damage in comparison with native ds-DNA are discussed as a comparative analysis for the first time. A single lesion—8-oxo-7,8-dihydro-2′-deoxyguanosine (Goxo), a tandem lesion—(5′S) and (5′R) 5′,8-cyclo-2′-deoxyadenosine (cdA), and the presence of ...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Boles ław T. Karwowski Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3127: Saccharomyces bayanus Enhances Volatile Profile of Apple Brandies
Tarko Qualitative and quantitative profiles of volatiles in alcoholic beverages depend mainly on the quality of raw materials, yeasts used for fermentation, and processing technique. Saccharomyces bayanus is a yeast species which is not commonly used for the production of alcoholic beverages, but it is able to produce volatiles that add desirable aroma. Since there is little information regarding the application of that microorganism for the production of apple brandies and how it affects volatile profile of finished products, we decided to address that issue. The aim of the study was to determine the impact of S. bay...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Magdalena Januszek Pawe ł Satora Łukasz Wajda Tomasz Tarko Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3128: Fermented Ginseng Extract, BST204, Suppresses Tumorigenesis and Migration of Embryonic Carcinoma through Inhibition of Cancer Stem Cell Properties
In this study, we identified the anti-tumorigenic and anti-invasive activities of BST204 through the suppression of the cancer stem cell marker, CD133. The treatment of embryonic carcinoma cells with BST204 induced the expression of the tumor suppressor protein, p53, which decreased the expression of cell cycle regulatory proteins and downregulated the expression of CD133 and several stemness transcription factors. These changes resulted in both the inhibition of tumor cell proliferation and tumorigenesis. The knockdown of CD133 suggests that it has a role in tumorigenesis, but not in cancer cell proliferation or cell cycl...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Jong Woo Park Jee Hun Park Jeung-Whan Han Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3129: Effects of the Sintering Process on Nacre-Derived Hydroxyapatite Scaffolds for Bone Engineering
This study aimed to compare the morphology and bioactivity of sinter and nonsinter marine-based hydroxyapatite scaffolds. Field emission scanning electron microscopy (FESEM) and micro-computed tomography (microCT) were used to characterize the morphology of both scaffolds. Scaffolds were co-cultured with 5 × 104/cm2 of MC3T3-E1 preosteoblast cells for 7, 14, and 21 days. FESEM was used to observe the cell morphology, and MTT and alkaline phosphatase (ALP) assays were conducted to determine the cell viability and differentiation capacity of cells on both scaffolds. Real-time polymerase chain reaction (rtPCR) w...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Rohaya Megat Abdul Wahab Nurmimie Abdullah Shahrul Hisham Zainal Ariffin Che Azurahanim Che Abdullah Farinawati Yazid Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3130: RNA Granules: A View from the RNA Perspective
Trcek RNA granules are ubiquitous. Composed of RNA-binding proteins and RNAs, they provide functional compartmentalization within cells. They are inextricably linked with RNA biology and as such are often referred to as the hubs for post-transcriptional regulation. Much of the attention has been given to the proteins that form these condensates and thus many fundamental questions about the biology of RNA granules remain poorly understood: How and which RNAs enrich in RNA granules, how are transcripts regulated in them, and how do granule-enriched mRNAs shape the biology of a cell? In this review, we discuss the imagin...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Tian Curnutte Trcek Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3131: Porin from Marine Bacterium Marinomonas primoryensis KMM 3633T: Isolation, Physico-Chemical Properties, and Functional Activity
lov’eva Voronina Marinomonas primoryensis KMM 3633T, extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633T (MpOmp) has been isolated and characterized. Amino acid analysis and whole genome sequencing were the sources of amino acid data of porin, identified as Porin_4 according to the conservative domain searching. The amino acid composition of MpOmp distinguished by high content of acidic amino acids and low c...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Novikova Khomenko Kim Likhatskaya Romanenko Aksenova Kunda Ryzhova Portnyagina Solov ’eva Voronina Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3132: Extraction of Ibuprofen from Natural Waters Using a Covalent Organic Framework
Djamila Salonen Espiña Ibuprofen is one of the most widely used pharmaceuticals, and due to its inefficient removal by conventional wastewater treatment, it can be found in natural surface waters at high concentrations. Recently, we demonstrated that the TpBD-(CF3)2 covalent organic framework (COF) can adsorb ibuprofen from ultrapure water with high efficiency. Here, we investigate the performance of the COF for the extraction of ibuprofen from natural water samples from a lake, river, and estuary. In general, the complexity of the natural water matrix induced a reduction in the adsorption efficiency of i...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Fernandes Mellah Kov ář S árria P šenička Djamila Salonen Espi ña Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3133: Discovery of 4-Anilinoquinolinylchalcone Derivatives as Potential NRF2 Activators
In this study, we have synthesized a series of 4-anilinoquinolinylchalcone derivatives, and used a NRF2 promoter-driven firefly luciferase reporter stable cell line, the HaCaT/ARE cells, to screen a panel of these compounds. Among them, (E)-3-{4-[(4-acetylphenyl)amino]quinolin-2-yl}-1-(4-fluorophenyl)prop-2-en-1-one (13b) significantly increased NRF2 activity in the HaCaT cell with a half maximal effective concentration (EC50) value of 1.95 μM. Treatment of compound 13b upregulated HaCaT cell NRF2 expression at the protein level. Moreover, the mRNA level of NRF2 target genes, heme oxygenase-1 (HO-1), glutamate-c...
Source: Molecules - July 8, 2020 Category: Chemistry Authors: Kao Chen Tang Lee Tseng Tzeng Yen Chen Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3098: Unravelling the Potential of Salivary Volatile Metabolites in Oral Diseases. A Review
cute; S. Câmara Fostered by the advances in the instrumental and analytical fields, in recent years the analysis of volatile organic compounds (VOCs) has emerged as a new frontier in medical diagnostics. VOCs analysis is a non-invasive, rapid and inexpensive strategy with promising potential in clinical diagnostic procedures. Since cellular metabolism is altered by diseases, the resulting metabolic effects on VOCs may serve as biomarkers for any given pathophysiologic condition. Human VOCs are released from biomatrices such as saliva, urine, skin emanations and exhaled breath and are derived from many metabolic p...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Jorge A. M. Pereira Priscilla Porto-Figueira Ravindra Taware Pritam Sukul Srikanth Rapole Jos é S. Câmara Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3099: Quasi-Isostructural Co(II) and Ni(II) Complexes with Mefenamato Ligand: Synthesis, Characterization, and Biological Activity
M. Wolf Three metal complexes of mefenamato ligand 1 were synthesized: [Co2(mef)4(EtOH)2(H2O)4]: 2; [Co(mef)2(MeOH)4]∙2MeOH: 3; and [Ni(mef)2(MeOH)4]∙2MeOH: 4. Their compositions and properties were investigated by elemental analysis (EA), flame atomic absorption spectrometry (FAAS), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). Crystal structures were determined by the single crystal X-ray diffraction technique. Additionally, their antioxidant and antimicrobial activity were established, thus proving good/moderate bioactivity against Gram-positive bacteria and yeasts. In the...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Micha ł Gacki Karolina Kafarska Anna Pietrzak Izabela Korona-G łowniak Wojciech M. Wolf Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3100: Molecular Features and Metal Ions that Influence 10-23 DNAzyme Activity
Ingrid Span Deoxyribozymes (DNAzymes) with RNA hydrolysis activity have a tremendous potential as gene suppression agents for therapeutic applications. The most extensively studied representative is the 10-23 DNAzyme consisting of a catalytic loop and two substrate binding arms that can be designed to bind and cleave the RNA sequence of interest. The RNA substrate is cleaved between central purine and pyrimidine nucleotides. The activity of this DNAzyme in vitro is considerably higher than in vivo, which was suggested to be related to its divalent cation dependency. Understanding the mechanism of DNAzyme catalysis is h...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Hannah Rosenbach Julian Victor Manuel Etzkorn Gerhard Steger Detlev Riesner Ingrid Span Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3101: The Inhibitory Activity of Anthraquinones Against Pathogenic Protozoa, Bacteria, and Fungi and the Relationship to Structure
g Kirkwood M. Land Plant-derived anthraquinones were evaluated in cell assays for their inhibitory activities against the parasitic protozoa Trichomonas vaginalis human strain G3 that causes the sexually transmitted disease trichomoniasis in women, Tritrichomonas foetus bovine strain D1 that causes sexually transmitted diseases in farm animals (bulls, cows, and pigs), Tritrichomonas foetus-like strain C1 that causes diarrhea in domestic animals (cats and dogs), and bacteria and fungi. The anthraquinones assessed for their inhibitory activity were anthraquinone, aloe-emodin (1,8-dihydroxy-3-hydroxymethylanthraquinone)...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Mendel Friedman Alexander Xu Rani Lee Daniel N. Nguyen Tina A. Phan Sabrina M. Hamada Rima Panchel Christina C. Tam Jong H. Kim Luisa W. Cheng Kirkwood M. Land Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3093: The GABAB Receptor —Structure, Ligand Binding and Drug Development
Molecules, Vol. 25, Pages 3093: The GABAB Receptor—Structure, Ligand Binding and Drug Development Molecules doi: 10.3390/molecules25133093 Authors: Linn Samira Mari Evenseth Mari Gabrielsen Ingebrigt Sylte The γ-aminobutyric acid (GABA) type B receptor (GABAB-R) belongs to class C of the G-protein coupled receptors (GPCRs). Together with the GABAA receptor, the receptor mediates the neurotransmission of GABA, the main inhibitory neurotransmitter in the central nervous system (CNS). In recent decades, the receptor has been extensively studied with the intention being to understand pathophysio...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Linn Samira Mari Evenseth Mari Gabrielsen Ingebrigt Sylte Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3095: Adsorption Capacity of Vitamin B12 and Creatinine on Highly-Mesoporous Activated Carbons Obtained from Lignocellulosic Raw Materials
Andrei Rotaru Enterosorbents are widely-used materials for human body detoxification, which function by immobilizing and eliminating endogenous and exogenous toxins. Here, activated carbons, obtained from the lignocellulosic raw vegetal materials of indigenous provenance, have been studied. Walnut shell and wood from local species of nuts and apple-trees were carbonized, and further activated at high temperatures with water vapors in a rotary kiln. A second activation was carried out, in a fluidized bed reactor, but for shorter times. The textural properties of the samples were determined from the adsorption isotherms ...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Tudor Lupa şcu Oleg Petuhov Nina Ţîmbaliuc Silvia Cibotaru Andrei Rotaru Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3094: Thiyl Radicals: Versatile Reactive Intermediates for Cyclization of Unsaturated Substrates
lan Sulfur centered radicals are widely employed in chemical synthesis, in particular for alkene and alkyne hydrothiolation towards thioether bioconjugates. The steadfast radical chain process that enables efficient hydrothiolation has been explored in the context of cascade reactions to furnish complex molecular architectures. The use of thiyl radicals offers a much cheaper and less toxic alternative to the archetypal organotin-based radical methods. This review outlines the development of thiyl radicals as reactive intermediates for initiating carbocyclization cascades. Key developments in cascade cyclization methodo...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Dylan M. Lynch Eoin M. Scanlan Tags: Review Source Type: research

Molecules, Vol. 25, Pages 3102: Granzyme B PET Imaging of the Innate Immune Response
Molotkov The human immune system is a complex system which protects against invaders and maintains tissue homeostasis. It is broadly divided into the innate and adaptive branches. Granzyme B is serine protease that plays an important role in both and can serve as a biomarker for cellular activation. Because of this, a granzyme B PET agent (GZP) has recently been developed and has been shown to be able to monitor response to immunotherapy. Here, we evaluated the utility of granzyme B PET imaging to assess the innate immune response. We subcutaneously administered LPS to mice to induce inflammation and performed granzym...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Kathleen M. Capaccione Mikhail Doubrovin Nikunj Bhatt Akiva Mintz Andrei Molotkov Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3097: Anticancer Potential and Capsianosides Identification in Lipophilic Fraction of Sweet Pepper (Capsicum annuum L.)
This study aimed to determine the health-promoting properties of sweet pepper by comparing the activity of fractions with variable lipophilicity. Fractions from red pericarp: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3) were analyzed for antiradical activity (with DPPH• and ABTS+•), and the contents of total phenolic compounds (TP), flavonoids (TF), and dihydroxycinnamic acids (TDHCA). The anticancer potential of the fractions was evaluated in vitro using different cancer cell lines: human colorectal carcinoma (HCT116) and PC-3 (prostate cancer cell). Fibroblast-like cells of L929 obtained from ...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Barbara Chilczuk Beata Marciniak Anna Stochmal Łukasz Pecio Renata Kontek Izabella Jackowska Ma łgorzata Materska Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3096: A Nano Drug Delivery System Based on Angelica sinensis Polysaccharide for Combination of Chemotherapy and Immunotherapy
Hong Yang Combination of chemotherapy and immunotherapy has been a promising strategy in cancer treatment. Polysaccharides from Angelica sinensis (AP), a well-known Chinese herbal medicine, have been proved to have good immunomodulatory activity. In the present study, an enzyme-sensitive tumor-targeting nano drug delivery system (AP-PP-DOX (doxorubicin), PP stood for peptide) was constructed. In this system, Angelica polysaccharides act as not only carriers to targeted delivery of drugs to tumor tissue but also effectors to improve tumor microenvironment and enhance immune function, resulting in synergistic antitumor e...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Min-Zhe Wang Xin He Zhe Yu Hong Wu Tie-Hong Yang Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3103: Design, Synthesis and Biological Evaluation of Novel Anthraniloyl-AMP Mimics as PQS Biosynthesis Inhibitors Against Pseudomonas aeruginosa Resistance
aresh Kumar The Pseudomonas quinolone system (PQS) is one of the three major interconnected quorum sensing signaling systems in Pseudomonas aeruginosa. The virulence factors PQS and HHQ activate the transcription regulator PqsR (MvfR), which controls several activities in bacteria, including biofilm formation and upregulation of PQS biosynthesis. The enzyme anthraniloyl-CoA synthetase (PqsA) catalyzes the first and critical step in the biosynthesis of quinolones; therefore, it is an attractive target for the development of anti-virulence therapeutics against Pseudomonas resistance. Herein, we report the design and synt...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Shekh Sabir Sujatha Subramoni Theerthankar Das David StC. Black Scott A. Rice Naresh Kumar Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3104: Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages
Kim Man-Deuk Han The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effect...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Nam-Hun Kim Hong-Ki Kim Ji-Hak Lee Seung-Il Jo Hye-Min Won Gyeong-Seok Lee Hyoun-Su Lee Kung-Woo Nam Wan-Jong Kim Man-Deuk Han Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3087: Characteristic Metabolic Changes of the Crust from Dry-Aged Beef Using 2D NMR Spectroscopy
Cheorun Jo Two-dimensional quantitative nuclear magnetic resonance (2D qNMR)-based metabolomics was performed to understand characteristic metabolic profiles in different aging regimes (crust from dry-aged beef, inner edible flesh of dry-aged beef, and wet-aged beef striploin) over 4 weeks. Samples were extracted using 0.6 M perchlorate to acquire polar metabolites. Partial least squares-discriminant analysis showed a good cumulative explained variation (R2 = 0.967) and predictive ability (Q2 = 0.935). Metabolites of crust and aged beef (dry- and wet-aged beef) were separated in the first week and showed a completely ...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Hyun Cheol Kim Ki Ho Baek Yoon-Joo Ko Hyun Jung Lee Dong-Gyun Yim Cheorun Jo Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3086: Accumulation of Saponins in Underground Parts of Panax vietnamensis at Different Ages Analyzed by HPLC-UV/ELSD
In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 ...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Kim Long Vu-Huynh Huy Truong Nguyen Thi Hong Van Le Chi Thanh Ma Gwang Jin Lee Sung Won Kwon Jeong Hill Park Minh Duc Nguyen Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3088: Antioxidant Activity of Selected Phenolic Acids –Ferric Reducing Antioxidant Power Assay and QSAR Analysis of the Structural Features
We present experimental and theoretical studies on the antioxidant potential of the set of 22 phenolic acids with different models of hydroxylation and methoxylation of aromatic rings. Ferric reducing antioxidant power assay was used to evaluate this property. 2,3-dihydroxybenzoic acid was found to be the strongest antioxidant, while mono hydroxylated and methoxylated structures had the lowest activities. A comprehensive structure–activity investigation with density functional theory methods elucidated the influence of compounds topology, resonance stabilization, and intramolecular hydrogen bonding on the exh...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Maciej Spiegel Karina Kapusta Wojciech Ko łodziejczyk Julia Saloni Beata Żbikowska Glake A. Hill Zbigniew Sroka Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3090: Assembly and Redox-Rich Hydride Chemistry of an Asymmetric Mo2S2 Platform
We report herein the synthesis, reactions and spectroscopic properties of a molybdenum-hydride complex featuring an asymmetric Mo2S2 core. This molecule displays rich redox chemistry with electrochemical couples at E½ = −0.45, −0.78 and −1.99 V vs. Fc/Fc+. The corresponding hydrido-complexes for all three redox levels were isolated and characterized crystallographically. Through an analysis of solid-state bond metrics and DFT calculations, we show that the electron-transfer processes for the two more positive couples are centered predominantly on the pyridinediimine ...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Alex McSkimming Jordan W. Taylor W. Hill Harman Tags: Article Source Type: research

Molecules, Vol. 25, Pages 3091: Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics
This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-V...
Source: Molecules - July 7, 2020 Category: Chemistry Authors: Vivek K. Chaturvedi Navneet Yadav Neeraj K. Rai Noura H. Abd Ellah Raghvendra A. Bohara Ibrahim F. Rehan Najat Marraiki Gaber El-Saber Batiha Helal F. Hetta M. P. Singh Tags: Article Source Type: research