Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria
Microorganisms. 2024 Apr 18;12(4):820. doi: 10.3390/microorganisms12040820.ABSTRACTThe spread of antibiotic-resistant bacteria and the rise of emerging and re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies to overcome these challenges. Herein, we describe an innovative method to synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation in Liquid (PLAL-AgNPs). Thus produced, nanoparticles were characterized by total X-ray fluorescence, zeta potential analysis, transmission electron microscopy (TEM), and nanoparticl...
Source: Herpes - April 27, 2024 Category: Infectious Diseases Authors: Maria Vittoria Morone Annalisa Chianese Federica Dell'Annunziata Veronica Folliero Erwin Pavel Lamparelli Giovanna Della Porta Carla Zannella Anna De Filippis Gianluigi Franci Massimiliano Galdiero Antonio Morone Source Type: research
Antimicrobial Efficacy of a Vegetable Oil Plasticizer in PVC Matrices
Polymers (Basel). 2024 Apr 10;16(8):1046. doi: 10.3390/polym16081046.ABSTRACTThe growing prevalence of bacterial and viral infections, highlighted by the recent COVID-19 pandemic, urgently calls for new antimicrobial strategies. To this end, we have synthesized and characterized a novel fatty acid epoxy-ester plasticizer for polymers, named GDE. GDE is not only sustainable and user-friendly but also demonstrates superior plasticizing properties, while its epoxy components improve the heat stability of PVC-based matrices. A key feature of GDE is its ability to confer antimicrobial properties to surfaces. Indeed, upon contac...
Source: Herpes - April 27, 2024 Category: Infectious Diseases Authors: Greta Bajetto Sara Scutera Francesca Menotti Giuliana Banche Giuseppe Chiaradia Caterina Turesso Marco De Andrea Marta Vallino Daan S Van Es Matteo Biolatti Valentina Dell'Oste Tiziana Musso Source Type: research
Prevalence and Resistance Patterns of Urinary Tract Infection in Al-Madinah Al-Munawarah, Saudi Arabia: A Retrospective Study
CONCLUSION: This study reported UT mono-infection and poly-infection in Al-Madinah Al-Munawarah, Saudi Arabia, with a predominant representation from gram-negative bacteria, Enterobacteriaceae. Most of the UT microbial strains showed a highly resistant profile.PMID:38665033 | DOI:10.24976/Discov.Med.202436183.80 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Areej A Alhhazmi Rawan A Alhazmi Eilaf S Alahmadi Wafa M Almuallim Zainab I Aljurfi Mohammad A Alturkostani Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Lipidomic approach to identify Escherichia coli and Shigella spp. by matrix-assisted laser desorption/ionization mass spectrometry
CONCLUSIONS: Identification of bacteria from lipid fingerprints obtained by the MALDI MS technique is possible and may become a useful tool in the future, especially for microorganisms that are difficult to distinguish by other methods.PMID:38670227 | DOI:10.1016/j.advms.2024.04.005 (Source: Advances in Medical Sciences)
Source: Advances in Medical Sciences - April 26, 2024 Category: Biomedical Science Authors: Adrian Arendowski Source Type: research
A new reagent for in vivo structure probing of RNA G and U residues that improves RNA structure prediction alone and combined with DMS
RNA. 2024 Apr 26:rna.079974.124. doi: 10.1261/rna.079974.124. Online ahead of print.ABSTRACTA key to understanding the roles of RNA in regulating gene expression is knowing their structures in vivo. One way to obtain this information is through probing structures of RNA with chemicals. To probe RNA structure directly in cells, membrane-permeable reagents that modify the Watson-Crick (WC) face of unpaired nucleotides can be used. While dimethyl sulfate (DMS) has led to substantial insight into RNA structure, it has limited nucleotide specificity in vivo, with WC face reactivity only at adenine (A) and cytosine (C) at neutra...
Source: RNA - April 26, 2024 Category: Genetics & Stem Cells Authors: Catherine A Douds Paul Babitzke Philip Bevilacqua Source Type: research
Detection of OXA-181 Carbapenemase in Shigella flexneri
We report the detection of OXA-181 carbapenemase in an azithromycin-resistant Shigella spp. bacteria in an immunocompromised patient. The emergence of OXA-181 in Shigella spp. bacteria raises concerns about the global dissemination of carbapenem resistance in Enterobacterales and its implications for the treatment of infections caused by Shigella bacteria.PMID:38666725 | DOI:10.3201/eid3005.231558 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Ghulam Dhabaan Hassan Jamal Danielle Ouellette Sarah Alexander Karen Arane Aaron Campigotto Manal Tadros Pierre-Philippe Pich é-Renaud Source Type: research
Antigenic Characterization of Novel Human Norovirus GII.4 Variants San Francisco 2017 and Hong Kong 2019
Emerg Infect Dis. 2024 May;30(5):1026-1029. doi: 10.3201/eid3005.231694.ABSTRACTNorovirus is a major cause of acute gastroenteritis; GII.4 is the predominant strain in humans. Recently, 2 new GII.4 variants, Hong Kong 2019 and San Francisco 2017, were reported. Characterization using GII.4 monoclonal antibodies and serum demonstrated different antigenic profiles for the new variants compared with historical variants.PMID:38666659 | DOI:10.3201/eid3005.231694 (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Kentaro Tohma Michael Landivar Lauren A Ford-Siltz Kelsey A Pilewski Joseph A Kendra Sandra Niendorf Gabriel I Parra Source Type: research
Antimicrobial Resistance as Risk Factor for Recurrent Bacteremia after Staphylococcus aureus, Escherichia coli, or Klebsiella spp. Community-Onset Bacteremia
Emerg Infect Dis. 2024 May;30(5):974-983. doi: 10.3201/eid3005.231555.ABSTRACTWe investigated links between antimicrobial resistance in community-onset bacteremia and 1-year bacteremia recurrence by using the clinical data warehouse of Europe's largest university hospital group in France. We included adult patients hospitalized with an incident community-onset Staphylococcus aureus, Escherichia coli, or Klebsiella spp. bacteremia during 2017-2019. We assessed risk factors of 1-year recurrence using Fine-Gray regression models. Of the 3,617 patients included, 291 (8.0%) had >1 recurrence episode. Third-generation cephalo...
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: Salam Abbara Didier Guillemot David R M Smith Salma El Oualydy Maeva Kos C écile Poret St éphane Breant Christian Brun-Buisson Laurence Watier Source Type: research
Economic Burden of Acute Gastroenteritis among Members of Integrated Healthcare Delivery System, United States, 2014-2016
Emerg Infect Dis. 2024 May;30(5):968-973. doi: 10.3201/eid3005.230356.ABSTRACTWe conducted a large surveillance study among members of an integrated healthcare delivery system in Pacific Northwest of the United States to estimate medical costs attributable to medically attended acute gastroenteritis (MAAGE) on the day care was sought and during 30-day follow-up. We used multivariable regression to compare costs of MAAGE and non-MAAGE cases matched on age, gender, and index time. Differences accounted for confounders, including race, ethnicity, and history of chronic underlying conditions. Analyses included 73,140 MAAGE epi...
Source: Emerging Infectious Diseases - April 26, 2024 Category: Infectious Diseases Authors: John F Dickerson Suzanne B Salas Judy Donald Holly C Groom Mi H Lee Claire P Mattison Aron J Hall Mark A Schmidt Source Type: research
Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys
Vaccine. 2024 Apr 25:S0264-410X(24)00483-3. doi: 10.1016/j.vaccine.2024.04.056. Online ahead of print.ABSTRACTGroup A rotavirus (RVA) is the primary etiological agent of acute gastroenteritis (AGE) in children under 5 years of age. Despite the global implementation of vaccines, rotavirus infections continue to cause over 120,000 deaths annually, with a majority occurring in developing nations. Among infants, the P[8] rotavirus strain is the most prevalent and can be categorized into four distinct lineages. In this investigation, we expressed five VP4(aa26-476) proteins from different P[8] lineages of human rotavirus in E. ...
Source: Vaccine - April 26, 2024 Category: Allergy & Immunology Authors: Guoxing Luo Yuanjun Zeng Roufang Sheng Zhishan Zhang Cao Li Han Yang Yaling Chen Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia Source Type: research
Prevalence and Resistance Patterns of Urinary Tract Infection in Al-Madinah Al-Munawarah, Saudi Arabia: A Retrospective Study
CONCLUSION: This study reported UT mono-infection and poly-infection in Al-Madinah Al-Munawarah, Saudi Arabia, with a predominant representation from gram-negative bacteria, Enterobacteriaceae. Most of the UT microbial strains showed a highly resistant profile.PMID:38665033 | DOI:10.24976/Discov.Med.202436183.80 (Source: Discovery Medicine)
Source: Discovery Medicine - April 26, 2024 Category: Research Authors: Areej A Alhhazmi Rawan A Alhazmi Eilaf S Alahmadi Wafa M Almuallim Zainab I Aljurfi Mohammad A Alturkostani Source Type: research
Surface Engineering of < em > Escherichia coli < /em > to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research
Lipidomic approach to identify Escherichia coli and Shigella spp. by matrix-assisted laser desorption/ionization mass spectrometry
CONCLUSIONS: Identification of bacteria from lipid fingerprints obtained by the MALDI MS technique is possible and may become a useful tool in the future, especially for microorganisms that are difficult to distinguish by other methods.PMID:38670227 | DOI:10.1016/j.advms.2024.04.005 (Source: Advances in Medical Sciences)
Source: Advances in Medical Sciences - April 26, 2024 Category: Biomedical Science Authors: Adrian Arendowski Source Type: research
Identification of a homoarginine biosynthetic gene from a microcystin biosynthetic pathway
This study provides new insights into the logic underpinning the biosynthesis of microcystin chemical variants and broadens our knowledge of structural diversity of microcystins.PMID:38670499 | DOI:10.1016/j.toxicon.2024.107733 (Source: Toxicon)
Source: Toxicon - April 26, 2024 Category: Toxicology Authors: Xiaodan Ouyang Matti Wahlsten Maija Pollari Endrews Delbaje Jouni Jokela David P Fewer Source Type: research
