Lipase and Protease Production Ability of Multi-drug Resistant Bacteria Worsens the Outcomes of Wound Infections
CONCLUSION: In conclusion, the findings suggest that lipases and proteases produced by bacteria could contribute to drug resistance and act as virulence factors in the development of surgical site infections. Understanding the role of these enzymes may inform strategies for preventing and managing post-surgical wound infections more effectively.PMID:38629357 | DOI:10.2174/0113816128302189240402043330 (Source: Current Pharmaceutical Design)
Source: Current Pharmaceutical Design - April 17, 2024 Category: Drugs & Pharmacology Authors: Attaur Rahman Saiqa Sardar Zeeshan Niaz Asif Khan None Sheheryar Abdulwahed Fahad Alrefaei Muhammad Hamayun Sajid Ali Source Type: research
Co-Immobilization of ADH and GDH on Metal-organic-framework: An Effective Biocatalyst for Asymmetric Reduction of Ketones
Chembiochem. 2024 Apr 17:e202400147. doi: 10.1002/cbic.202400147. Online ahead of print.ABSTRACTChiral alcohols are not only important building blocks of various bioactive natural compounds and pharmaceuticals, but can serve as synthetic precursors for other valuable organic chemicals, thus the synthesis of these products is of great importance. Bio-catalysis represents one effective way to obtain these molecules, however, the weak stability and high cost of enzymes often hinder its broad application. In this work, we designed a biological nanoreactor by embedding alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH)...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Lu Ran Guorong Su Zhenyan Yang Shuke Wu Huailong Teng Source Type: research
In vitro antibacterial activity of antibiotics and plant essential oils against Escherichia coli MTCC443 supported through the molecular docking and pharmacokinetics study
This study has been made to compare the in vitro antibacterial activity and further validated the same through the molecular docking study of 13 antibiotics such as ciprofloxacin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, kanamycin, vancomycin, streptomycin, penicillin, nalidixic acid, trimethoprim, and polymyxin, and 10 EOs such as garlic, tulsi, neem, clove, thyme, peppermint, coriander, tea, lavender, and eucalyptus against the target protein (DNA gyrase) ofE. coli MTCC443.E. coli Microbial Type Culture Collection 443 was found to be highly sensitive to ciprofloxacin (zone of inhibition [ZOI], 2...
Source: Biotechnology and Applied Biochemistry - April 17, 2024 Category: Biochemistry Authors: Saurabh Jain,
Adarsh Kumar Shukla,
Surbhi Panwar,
Rajesh Kumar,
Ashwani Kumar Tags: ORIGINAL ARTICLE Source Type: research
Effect of different levels of single cell protein and probiotic microorganisms on performance, immunological responses, and intestinal histology in laying hens
AbstractAn experiment was conducted to evaluate the effects of single-cell protein (SCP), as a valuable by-product and substitute for soybean meal, and multistrain probiotics on the performance, blood biochemistry, intestinal histomorphology, ileal microbiota, and immune responses in laying hens. Leghorn laying hens were randomly assigned to nine experimental treatments with eight replicates of five birds each. Dietary treatments consisted of three levels of replacement of soybean meal with SCP (0.0%, 30%, and 60%) and three levels of probiotics (0.0, 50, 100 mg/kg) offered through 70 days of main recording. The result ...
Source: Journal of Animal Physiology and Animal Nutrition - April 17, 2024 Category: Zoology Authors: Rasoul Rashedi Dehsahraee,
Amir Hossein Mahdavi,
Mohammad Sedghi,
Hassan Saleh Tags: ORIGINAL ARTICLE Source Type: research
Spirulina (Arthrospira platensis) supplementation: Impact on growth, metabolism, and antioxidant status in zebrafish
In conclusion, this study indicates that SP supplementation has substantial potential as a growth promoter, positively influencing lipid metabolism and antioxidant enzyme activity without affecting zebrafish survival. (Source: Journal of Animal Physiology and Animal Nutrition)
Source: Journal of Animal Physiology and Animal Nutrition - April 17, 2024 Category: Zoology Authors: Aline Pereira Coli,
William Franco Carneiro,
Kiara C ândido Duarte da Silva,
Tássia Flávia Dias Castro,
João Paulo Lima de Oliveira,
Moisés Silvestre Azevedo de Martins,
Luis David Solis Murgas Tags: ORIGINAL ARTICLE Source Type: research
GSE233657 Robust Host Engineering: Enhancing Escherichia coli ´s Abiotic Stress Resistance through Ornithine Lipid Membrane Modification
Contributors : Leidy P Bedoya P érez ; Omar A Aguilar Vera ; José Utrilla Carreri ; Christian SohlenkampSeries Type : Expression profiling by high throughput sequencingOrganism : Escherichia coliTranscriptome analysis revealed that strains had differentially expressed stress and membrane-related genes compared to the control strain (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Escherichia coli Source Type: research
Mobile Colistin Resistance and Plasmid-Mediated Quinolone Resistance Genes in Escherichia coli from China, 1993 –2019
Foodborne Pathogens and Disease, Ahead of Print. (Source: Foodborne Pathogens and Disease)
Source: Foodborne Pathogens and Disease - April 17, 2024 Category: Food Science Authors: Yujin Wang Dewei Sun Zhengzhong Xu Xin'an Jiao Xiang Chen Source Type: research
Molecules, Vol. 29, Pages 1829: Phytochemical Analysis, Antimicrobial Screening and In Vitro Pharmacological Activity of Artemisia vestita Leaf Extract
jay Yadav
Artemisia vestita Wall. Ex Besser is a folklore medicinal plant that belongs to Asteraceae family and a treasure trove of drugs. The aim of this research study was to investigate the phytoconstituents, antimicrobial activity, antioxidant, anti-inflammatory, cytotoxicity and wound healing potential of A. vestita leaf extract (ALE). Phytochemical analysis of the ALE was carried out by Soxhlet extraction and GCMS (gas chromatography–mass spectrometry) analysis. Antimicrobial activity was performed by the agar well diffusion method against selected bacterial and fungal strains. Free radical scavengi...
Source: Molecules - April 17, 2024 Category: Chemistry Authors: Shivani Dogra Bhupendra Koul Joginder Singh Meerambika Mishra Dhananjay Yadav Tags: Article Source Type: research
Molecules, Vol. 29, Pages 1831: Isolation of Extracellular Outer Membrane Vesicles (OMVs) from Escherichia coli Using EVscore47 Beads
In this study, EVscore47 multifunctional chromatography microspheres were synthesized and used to efficiently isolate functional OMVs from Escherichia coli. Through this technology, OMV loss can be kept to a minimum, and OMVs can be harvested using EVscore47 at 11-fold higher yields and ~13-fold higher purity than those achieved by means of ultracentrifugation. Based on the results presented here, we propose a novel EVscore47-based isolation of OMVs that is fast and scalable. (Source: Molecules)
Source: Molecules - April 17, 2024 Category: Chemistry Authors: Gongming Shi Xiaohong Yang Jikai Wang Wenjing Wei Kecui Hu Xingyue Huang Yanfei Qiu Yun He Tags: Article Source Type: research
DNA glycosylases provide antiviral defence in prokaryotes
Nature, Published online: 17 April 2024; doi:10.1038/s41586-024-07329-9A screen utilizing an environmental DNA library in Escherichia coli is used to identify Brig1, a previously unknown anti-phage defence system with homologues across distinct clades of bacteria. (Source: Nature AOP)
Source: Nature AOP - April 17, 2024 Category: Research Authors: Amer A. Hossain Ying Z. Pigli Christian F. Baca S øren Heissel Alexis Thomas Vincent K. Libis J án Burian Joshua S. Chappie Sean F. Brady Phoebe A. Rice Luciano A. Marraffini Source Type: research
Activity assays of NnlA homologs suggest the natural product N-nitroglycine is degraded by diverse bacteria
Abstract
Linear nitramines (R–N(R′)NO2; R′ = H or alkyl) are toxic compounds, some with environmental relevance, while others are rare natural product nitramines. One of these natural product nitramines is N-nitroglycine (NNG), which is produced by some Streptomyces strains and exhibits antibiotic activity towards Gram-negative bacteria. An NNG degrading heme enzyme, called NnlA, has recently been discovered in the genome of Variovorax sp. strain JS1663 (Vs NnlA). Evidence is presented that NnlA and therefore, NNG degradation activity is widespread. To achieve this objective, we characterized and tested t...
Source: Beilstein Journal of Organic Chemistry - April 17, 2024 Category: Chemistry Authors: Beilstein Journal of Organic Chemistry - Syndicated Feeds Tags: enzymology natural products nitramine – N bond Full Research Paper Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: Am J Trop Med Hyg - April 16, 2024 Category: Infectious Diseases Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Lipoic acid attachment to proteins: stimulating new developments
Microbiol Mol Biol Rev. 2024 Apr 16:e0000524. doi: 10.1128/mmbr.00005-24. Online ahead of print.ABSTRACTSUMMARYLipoic acid-modified proteins are essential for central metabolism and pathogenesis. In recent years, the Escherichia coli and Bacillus subtilis lipoyl assembly pathways have been modified and extended to archaea and diverse eukaryotes including humans. These extensions include a new pathway to insert the key sulfur atoms of lipoate, several new pathways of lipoate salvage, and a novel use of lipoic acid in sulfur-oxidizing bacteria. Other advances are the modification of E. coli LplA for studies of protein locali...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: John E Cronan Source Type: research
Genomic characterization of ST38 NDM-5-producing < em > Escherichia coli < /em > isolates from an outbreak in the Czech Republic
Antimicrob Agents Chemother. 2024 Apr 16:e0013324. doi: 10.1128/aac.00133-24. Online ahead of print.ABSTRACTA 2-year national genomic screening in the Czech Republic identified a notable prevalence of the New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) in the city of Brno. Forty-two ST38 E. coli isolates harbored the blaNDM-5 gene on the chromosome. Virulence factors confirmed the persistence of these isolates through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal genomic variations, implying a clonally driven outb...
Source: Antimicrobial Agents and Chemotherapy - April 16, 2024 Category: Microbiology Authors: Katerina Chudejova Tsolaire Sourenian Jana Palkovicova Katarina Stredanska Lucie Nechutna Katerina Vlkova Vendula Studentova Working Group for Monitoring of Antibiotic Resistance Jaroslav Hrabak Costas C Papagiannitsis Monika Dolejska Ibrahim Bitar Workin Source Type: research
Identification and enzymatic properties of arginine decarboxylase from < em > Aspergillus oryzae < /em >
Appl Environ Microbiol. 2024 Apr 16:e0029424. doi: 10.1128/aem.00294-24. Online ahead of print.ABSTRACTAspergillus oryzae spores, when sprinkled onto steamed rice and allowed to propagate, are referred to as rice "koji." Agmatine, a natural polyamine derived from arginine through the action of arginine decarboxylase (ADC), is abundantly produced by solid state-cultivated rice koji of A. oryzae RIB40 under low pH conditions, despite the apparent absence of ADC orthologs in its genome. Mass spectrometry imaging revealed that agmatine was accumulated inside rice koji at low pH conditions, where arginine was distributed. ADC a...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Yui Murakami Soichiro Ikuta Wakao Fukuda Naoki Akasaka Jun-Ichi Maruyama Shuichi Shinma Eiichiro Fukusaki Shinsuke Fujiwara Source Type: research
