Renadirsen, a Novel 2'OMeRNA/ENA < sup > ® < /sup > Chimera Antisense Oligonucleotide, Induces Robust Exon 45 Skipping for Dystrophin In Vivo
Curr Issues Mol Biol. 2021 Sep 25;43(3):1267-1281. doi: 10.3390/cimb43030090.ABSTRACTDuchenne muscular dystrophy (DMD) is a progressive muscle-wasting disease caused by out-of-frame or nonsense mutation in the dystrophin gene. It begins with a loss of ambulation between 9 and 14 years of age, followed by various other symptoms including cardiac dysfunction. Exon skipping of patients' DMD pre-mRNA induced by antisense oligonucleotides (AOs) is expected to produce shorter but partly functional dystrophin proteins, such as those possessed by patients with the less severe Becker muscular dystrophy. We are working on developing...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Kentaro Ito Hideo Takakusa Masayo Kakuta Akira Kanda Nana Takagi Hiroyuki Nagase Nobuaki Watanabe Daigo Asano Ryoya Goda Takeshi Masuda Akifumi Nakamura Yoshiyuki Onishi Toshio Onoda Makoto Koizumi Yasuhiro Takeshima Masafumi Matsuo Kiyosumi Takaishi Source Type: research

Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells
In conclusion, our data suggest that the hair growth stimulating activity of D-panthenol was exerted by increasing the cell viability, suppressing the apoptotic markers, and elongating the anagen phase in hair follicles.PMID:34698060 | DOI:10.3390/cimb43030097 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Jae Young Shin Jaeyoon Kim Yun-Ho Choi Nae-Gyu Kang Sanghwa Lee Source Type: research

A Comparative Analysis of the Altered Levels of Human Seminal Plasma Constituents as Contributing Factors in Different Types of Male Infertility
In this study, we evaluated the potential of several seminal plasma biochemical and immunological markers in the pathophysiological developments of the infertile male patients. The study was designed to identify and assess different markers that may be associated with semen functions in different types of male infertility. (2) Methods: A total of 50 infertile male patients who underwent checkup for fertility assessment and 50 fertile controls were included in this study. The complete medical history of each recruited participant was reviewed. The infertile sub-groups (non-obstructive azoospermia (NOA), asthenozoospermia (A...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Ashutosh Vashisht Pankaj Kumar Ahluwalia Gagandeep Kaur Gahlay Source Type: research

Genistein: A Potent Anti-Breast Cancer Agent
Curr Issues Mol Biol. 2021 Oct 10;43(3):1502-1517. doi: 10.3390/cimb43030106.ABSTRACTGenistein is an isoflavonoid present in high quantities in soybeans. Possessing a wide range of bioactives, it is being studied extensively for its tumoricidal effects. Investigations into mechanisms of the anti-cancer activity have revealed many pathways including induction of cell proliferation, suppression of tyrosine kinases, regulation of Hedgehog-Gli1 signaling, modulation of epigenetic activities, seizing of cell cycle and Akt and MEK signaling pathways, among others via which the cancer cell proliferation can be controlled. Notwith...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Smitha S Bhat Shashanka K Prasad Chandan Shivamallu Kollur Shiva Prasad Asad Syed Pruthvish Reddy Charley A Cull Raghavendra G Amachawadi Source Type: research

Skeletal Muscle Regeneration by the Exosomes of Adipose Tissue-Derived Mesenchymal Stem Cells
This study aimed to determine the regenerative capacity of MSC-derived exosomes for skeletal muscle regeneration. Exosomes were isolated from human adipose tissue-derived MSCs (AD-MSCs). The effects of MSC-derived exosomes on satellite cells were investigated using cell viability, relevant genes, and protein analyses. Moreover, NOD-SCID mice were used and randomly assigned to the healthy control (n = 4), muscle defect (n = 6), and muscle defect + exosome (n = 6) groups. Muscle defects were created using a biopsy punch on the quadriceps of the hind limb. Four weeks after the surgery, the quadriceps muscles were harvested, w...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Seong-Eun Byun Changgon Sim Yoonhui Chung Hyung Kyung Kim Sungmoon Park Do Kyung Kim Seongmin Cho Soonchul Lee Source Type: research

A Potential Role of the CD47/SIRPalpha Axis in COVID-19 Pathogenesis
Curr Issues Mol Biol. 2021 Sep 22;43(3):1212-1225. doi: 10.3390/cimb43030086.ABSTRACTThe coronavirus SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Most SARS-CoV-2 infections are mild or even asymptomatic. However, a small fraction of infected individuals develops severe, life-threatening disease, which is caused by an uncontrolled immune response resulting in hyperinflammation. However, the factors predisposing individuals to severe disease remain poorly understood. Here, we show that levels of CD47, which is known to mediate immune escape in cancer and virus-infected cells, are elevated in SARS-CoV-2-infected ...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Katie-May McLaughlin Denisa Bojkova Joshua D Kandler Marco Bechtel Philipp Reus Trang Le Florian Rothweiler Julian U G Wagner Andreas Weigert Sandra Ciesek Mark N Wass Martin Michaelis Jindrich Cinatl Source Type: research

Effect of Azithromycin on Mineralized Nodule Formation in MC3T3-E1 Cells
Curr Issues Mol Biol. 2021 Oct 6;43(3):1451-1459. doi: 10.3390/cimb43030102.ABSTRACTAzithromycin displays immunomodulatory and anti-inflammatory effects in addition to broad-spectrum antimicrobial activity and is used to treat inflammatory diseases, including respiratory and odontogenic infections. Few studies have reported the effect of azithromycin therapy on bone remodeling processes. The aim of this study was to examine the effects of azithromycin on the osteogenic function of osteoblasts using osteoblast-like MC3T3-E1 cells. Cells were cultured in the presence of 0, 0.1, 1, and 10 µg/mL azithromycin, and cell pr...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Kengo Kato Manami Ozaki Kumiko Nakai Maki Nagasaki Junya Nakajima Ryosuke Koshi Hideki Tanaka Takayuki Kawato Morio Tonogi Source Type: research

Effect of < em > Ducrosia flabellifolia < /em > and < em > Savignya parviflora < /em > Extracts on Inhibition of Human Colon and Prostate Cancer Cell Lines
Curr Issues Mol Biol. 2021 Oct 10;43(3):1518-1528. doi: 10.3390/cimb43030107.ABSTRACTThe goal of this study was to investigate whether Ducrosia flabellifolia and Savignya parviflora methanol extract the have effect on colon and prostate cancer cell lines. Analysis of total content of phenolics and flavonoids of each plant extract was carried out. Cytotoxic effect, cell cycle analysis, induction of apoptosis and gene expression of Bcl-2 and Bax genes were studied. Obtained results indicated that, the plant extracts exhibit growth inhibition of used cancer cell lines and induced apoptosis as well as arresting of cell cycle. ...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Youssef Saeed Alghamdi Osama Moseilhy Saleh Nada Alqadri Mutaib Mosaued Mashraqi Omar Bahattab Nabil Saied Awad Source Type: research

Potential DPP IV Inhibitory Peptides from Dry-Cured Pork Loins after Hydrolysis: An In Vitro and In Silico Study
Curr Issues Mol Biol. 2021 Sep 27;43(3):1335-1349. doi: 10.3390/cimb43030095.ABSTRACTPeptidyl peptidase IV (DPP-IV) is a pharmacotherapeutic target in type 2 diabetes, and inhibitors of this enzyme are an important class of drugs for the treatment of type 2 diabetes. In the present study, peptides (
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Paulina K ęska Joanna Stadnik Source Type: research

Synthetic Oligopeptides Mimicking γ-Core Regions of Cysteine-Rich Peptides of < em > Solanum lycopersicum < /em > Possess Antimicrobial Activity against Human and Plant Pathogens
Curr Issues Mol Biol. 2021 Sep 24;43(3):1226-1242. doi: 10.3390/cimb43030087.ABSTRACTPlant cysteine-rich peptides (CRPs) represent a diverse group of molecules involved in different aspects of plant physiology. Antimicrobial peptides, which directly suppress the growth of pathogens, are regarded as promising templates for the development of next-generation pharmaceuticals and ecologically friendly plant disease control agents. Their oligopeptide fragments are even more promising because of their low production costs. The goal of this work was to explore the antimicrobial activity of nine short peptides derived from the &ga...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Marina P Slezina Ekaterina A Istomina Ekaterina V Kulakovskaya Tatiana N Abashina Tatyana I Odintsova Source Type: research

Molecular Profiling of DNA Methylation and Alternative Splicing of Genes in Skeletal Muscle of Obese Rabbits
Curr Issues Mol Biol. 2021 Oct 11;43(3):1558-1575. doi: 10.3390/cimb43030110.ABSTRACTDNA methylation and the alternative splicing of precursor messenger RNAs (pre-mRNAs) are two important genetic modification mechanisms. However, both are currently uncharacterized in the muscle metabolism of rabbits. Thus, we constructed the Tianfu black rabbit obesity model (obese rabbits fed with a 10% high-fat diet and control rabbits from 35 days to 70 days) and collected the skeletal muscle samples from the two groups for Genome methylation sequencing and RNA sequencing. DNA methylation data showed that the promoter regions of 599 gen...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Yanhong Li Jie Wang Mauricio A Elzo Huimei Fan Kun Du Siqi Xia Jiahao Shao Tianfu Lai Shenqiang Hu Xianbo Jia Songjia Lai Source Type: research

Crosstalk between Cancer Cells and Fibroblasts for the Production of Monocyte Chemoattractant Protein-1 in the Murine 4T1 Breast Cancer
Curr Issues Mol Biol. 2021 Oct 22;43(3):1726-1740. doi: 10.3390/cimb43030122.ABSTRACTThe chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) is shown to promote the progression of breast cancer. We previously identified cancer cell-derived granulocyte-macrophage colony-stimulating factor (GM-CSF) as a potential regulator of MCP-1 production in the murine 4T1 breast cancer, but it played a minimum role in overall MCP-1 production. Here, we evaluated the crosstalk between 4T1 cells and fibroblasts. When fibroblasts were co-cultured with 4T1 cells or stimulated with the culture supernatants of 4T1 cells (4T1-sup), MCP-1...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Mayu Imamura Tiantian Li Chunning Li Masayoshi Fujisawa Naofumi Mukaida Akihiro Matsukawa Teizo Yoshimura Source Type: research

Assessment of Bioavailability after In Vitro Digestion and First Pass Metabolism of Bioactive Peptides from Collagen Hydrolysates
Curr Issues Mol Biol. 2021 Oct 13;43(3):1592-1605. doi: 10.3390/cimb43030113.ABSTRACTCollagen hydrolysates (CHs) are composed of bioactive peptides (BAPs), which possess health enhancing properties. There is a knowledge gap regarding the bioavailability of these BAPs that involves intestinal transport and hepatic first pass effects. A simulated gastrointestinal model was used to generate digesta from two CHs (CH-GL and CH-OPT), which were applied to a novel transwell co-culture of human intestinal epithelium cell line-6 (HIEC-6) and hepatic (HepG2) cells to simulate in vivo conditions of absorption and first pass metabolis...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Christina E Larder Mich èle M Iskandar Stan Kubow Source Type: research

Gene Expression Analysis with No Sequence Data: Study on Reeves's Muntjac ( < em > Muntiacus reevesi < /em > )
In this study, we attempt to design primers for a real-time PCR analysis, basing on a comparative analysis of selected gene sequences of species related to Reeves's muntjac (Muntiacus reevesi) and by identifying highly conservative regions. Results of PCR products sequencing and their alignment with the GenBank collection show that all selected primers gave products highly similar (> 90%) to the intended target (among compared species), which led us to the conclusion that our primers may be used for further analyses of gene expression.PMID:34698093 | DOI:10.3390/cimb43030111 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Jadwiga Flaga Marcin Przyby ło Pawe ł Górka Source Type: research

Loss of Melanopsin (OPN4) Leads to a Faster Cell Cycle Progression and Growth in Murine Melanocytes
In this study, we found that murine Opn4KO melanocytes displayed a faster proliferation rate compared to Opn4WT melanocytes. Cell cycle population analysis demonstrated that OPN4KO melanocytes exhibited a faster cell cycle progression with reduced G0-G1, and highly increased S and slightly increased G2/M cell populations compared to the Opn4WT counterparts. Expression of specific cell cycle-related genes in Opn4KO melanocytes exhibited alterations that corroborate a faster cell cycle progression. We also found significant modification in gene and protein expression levels of important regulators of melanocyte physiology. P...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Leonardo Vin ícius Monteiro de Assis Maria Nath ália Moraes Davi Mendes Matheus Molina Silva Carlos Frederico Martins Menck Ana Maria de Lauro Castrucci Source Type: research

Association of Bitter Taste Receptor T2R38 Polymorphisms, Oral Microbiota, and Rheumatoid Arthritis
Curr Issues Mol Biol. 2021 Oct 9;43(3):1460-1472. doi: 10.3390/cimb43030103.ABSTRACTThe association of taste genetics and the oral microbiome in autoimmune diseases such as rheumatoid arthritis (RA) has not been reported. We explored a novel oral mucosal innate immune pathway involving the bitter taste G protein-coupled receptor T2R38. This case-control study aimed to evaluate whether T2R38 polymorphisms associate with the buccal microbial composition in RA. Genomic DNA was obtained from buccal swabs of 35 RA patients and 64 non-RA controls. TAS2R38 genotypes were determined by Sanger sequencing. The buccal microbiome was ...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Vivianne Cruz de Jesus Manu Singh Robert J Schroth Prashen Chelikani Carol A Hitchon Source Type: research

Signaling Pathway of Histamine H < sub > 1 < /sub > Receptor-Mediated Histamine H < sub > 1 < /sub > Receptor Gene Upregulation Induced by Histamine in U-373 MG Cells
Curr Issues Mol Biol. 2021 Sep 24;43(3):1243-1254. doi: 10.3390/cimb43030088.ABSTRACTHistamine H1 receptor (H1R) is one of the targets of histamine in the nervous system and the peripheral tissues. Protein kinase Cδ (PKCδ) signaling is involved in histamine-induced upregulation of H1R gene expression in HeLa cells. Histamine also upregulates H1R gene expression in U-373 MG cells. However, the molecular signaling of this upregulation is still unclear. Here, we investigated the molecular mechanism of histamine-induced H1R gene upregulation in U-373 MG cells. Histamine-induced H1R gene upregulation was inhibited b...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Hiroyuki Mizuguchi Yuko Miyamoto Takuma Terao Haruka Yoshida Wakana Kuroda Yoshiaki Kitamura Noriaki Takeda Hiroyuki Fukui Source Type: research

Vitamin D Receptor Gene Variants Susceptible to Osteoporosis in Arab Post-Menopausal Women
Curr Issues Mol Biol. 2021 Sep 27;43(3):1325-1334. doi: 10.3390/cimb43030094.ABSTRACTPost-menopausal osteoporosis (PMO) is a multifactorial bone disorder in elderly women. Various vitamin D receptor (VDR) gene variants have been studied and associated with osteoporosis in other populations, but not in a homogenous Arab ethnic group. Herein, the current study explores the association between VDR polymorphisms and susceptibility to osteoporosis in Saudi postmenopausal women. In total, 600 Saudi postmenopausal women (N = 300 osteoporosis; N = 300 control) were genotyped for VDR gene variants (rs7975232, rs1544410, rs731236) u...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Mohammed G A Ansari Abdul Khader Mohammed Kaiser A Wani Syed D Hussain Abdullah M Alnaami Saba Abdi Naji J Aljohani Nasser M Al-Daghri Source Type: research

Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299
Curr Issues Mol Biol. 2021 Sep 22;43(3):1203-1211. doi: 10.3390/cimb43030085.ABSTRACTNon-small cell lung cancer (NSCLC) is an aggressive lung cancer accounting for approximately 85% of all lung cancer patients. For the patients with Stages IIIA, IIIB, and IIIC, the 5-year survival is low though with the combination with radiotherapy and chemotherapy. In addition, the occurrence of tumor cells (repopulated tumors) that survive irradiation remains a challenge. In our previous report, we subcloned the radiation-surviving tumor cells (IR cells) using the human NSCLC cell line, H1299, and found that the expression of neuropilin...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Kaori Tsutsumi Ayaka Chiba Yuta Tadaki Shima Minaki Takahito Ooshima Haruka Takahashi Source Type: research

Antidiabetic Effect of Taxifolin in Cultured L6 Myotubes and Type 2 Diabetic Model KK-A < sup > y < /sup > /Ta Mice with Hyperglycemia and Hyperuricemia
Curr Issues Mol Biol. 2021 Sep 26;43(3):1293-1306. doi: 10.3390/cimb43030092.ABSTRACTMuscle is the largest tissue in our body and plays an important role in glucose homeostasis and hence diabetes. In the present study, we examined the effects of taxifolin (TXF) on glucose metabolism in cultured L6 muscle cells (myotubes) and in type 2 diabetic (T2D) model KK-Ay/Ta mice. TXF dose-dependently increased glucose uptake (GU) in L6 myotubes under the condition of insulin absence. This increase in GU was partially, but significantly canceled by TXF treatment in combination with either LY294002, an inhibitor of phosphatidylinosito...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Shinji Kondo Shin-Ichi Adachi Fumiaki Yoshizawa Kazumi Yagasaki Source Type: research

Effects of Radix Polygalae on Cognitive Decline and Depression in Estradiol Depletion Mouse Model of Menopause
Curr Issues Mol Biol. 2021 Oct 19;43(3):1669-1684. doi: 10.3390/cimb43030118.ABSTRACTPostmenopausal syndrome refers to symptoms caused by the gradual decrease in female hormones after mid-40 years. As a target organ of estrogen, decrease in estrogen causes various changes in brain function such as a decrease in choline acetyltransferase and brain-derived neurotrophic factor; thus, postmenopausal women experience cognitive decline and more depressive symptoms than age-matched men. Radix Polygalae has been used for memory boosting and as a mood stabilizer and its components have shown neuroprotective, antidepressant, and str...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Gaeul Han Junhyuk Choi Seung-Yun Cha Byung Il Kim Hee Kyung Kho Maeng-Jin Jang Mi Ae Kim Sungho Maeng Heeok Hong Source Type: research

Cytoskeletal Actin Structure in Osteosarcoma Cells Determines Metastatic Phenotype via Regulating Cell Stiffness, Migration, and Transmigration
In this study, we analyzed the cell stiffness of the low metastatic Dunn cell line and its highly metastatic LM8 subline, and compared actin organization, cell proliferation, and metastasis. Actin cytoskeleton, polymerization, stiffness, and other cellular properties were analyzed. The organization of the actin cytoskeleton was evaluated by staining F-actin with Alexa Fluor 488 phalloidin. Cell stiffness was measured using Atomic Force Microscopy (AFM). Cell proliferation, migration, invasion, and adhesion were also evaluated. All experiments were performed using mouse osteosarcoma cell lines cultured in the absence and pr...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Kouji Kita Kunihiro Asanuma Takayuki Okamoto Eiji Kawamoto Koichi Nakamura Tomohito Hagi Tomoki Nakamura Motomu Shimaoka Akihiro Sudo Source Type: research

Impact of Vitamin D Supplementation on Inflammatory Markers' Levels in Obese Patients
In conclusion, our study seems to contradict certain aspects of findings available in the literature regarding the vitamin D's impact.PMID:34698104 | DOI:10.3390/cimb43030114 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Micha ł Wiciński Mateusz Ozorowski Eryk W ódkiewicz Stephan Walter Otto Karol Kubiak Bartosz Malinowski Source Type: research

Evaluation of BCL6 and SIRT1 as Non-Invasive Diagnostic Markers of Endometriosis
Conclusions: These results suggest some potential of SIRT1 expression within serum as a predictor of advanced asymptomatic stages of endometriosis. Using immunohistochemistry (IHC) staining and H-SCORE values for the elevated BCL6 (and potentially SIRT1) levels in endometrial biopsy samples seems to have higher diagnostic potential based on the previously published studies.PMID:34698105 | DOI:10.3390/cimb43030096 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Alison M Sansone Brooke V Hisrich R Brandt Young William F Abel Zachary Bowens Bailey B Blair Avery T Funkhouser David P Schammel Lisa J Green Bruce A Lessey Anna V Blenda Source Type: research

Genome-Wide Survey Reveals the Microsatellite Characteristics and Phylogenetic Relationships of < em > Harpadon nehereus < /em >
In this study, the genome-wide survey dataset first produced using next-generation sequencing (NGS) was used to provide general information on the genome size, heterozygosity and repeat sequence ratio of H. nehereus. About 68.74 GB of high-quality sequence data were obtained in total and the genome size was estimated to be 1315 Mb with the 17-mer frequency distribution. The sequence repeat ratio and heterozygosity were calculated to be 52.49% and 0.67%, respectively. A total of 1,027,651 microsatellite motifs were identified and dinucleotide repeat was the most dominant simple sequence repeat (SSR) motif with a frequency o...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Tianyan Yang Xinxin Huang Zijun Ning Tianxiang Gao Source Type: research

Multiplexed DNA Methylation Analysis in Colorectal Cancer Using Liquid Biopsy and Its Diagnostic and Predictive Value
We report here the identification of DNA methylation biomarkers for CRC and give insights in our methylation-sensitive restriction enzyme coupled qPCR (MSRE-qPCR) system. Targeted microarrays were used to investigate the DNA methylation status of 360 cancer-associated genes. Validation was done by qPCR-based approaches. A focus was on investigating marker performance in cfDNA from 88 patients (44 CRC, 44 controls). Finally, the workflow was scaled-up to perform 180plex analysis on 110 cfDNA samples, to identify a DNA methylation signature for advanced colonic adenomas (AA). A DNA methylation signature (n = 44) was deduced ...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Walter Pulverer Kristi Kruusmaa Silvia Sch önthaler Jasmin Huber Marko Bitenc Thomas Bachleitner-Hofmann Jagdeep Singh Bhangu Rudolf Oehler Gerda Egger Andreas Weinh äusel Source Type: research

CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of < em > Colletotrichum gloeosporioides < /em > from < em > Hevea brasiliensis < /em >
Curr Issues Mol Biol. 2021 Oct 11;43(3):1548-1557. doi: 10.3390/cimb43030109.ABSTRACTColletotrichum gloeosporioides is the main causal agent of anthracnose in various plant species. Determining the molecular mechanisms underlying the pathogenicity and fungicide resistance of C. gloeosporioides could help build new strategies for disease control. The major facilitator superfamily (MFS) has multiple roles in the transport of a diverse range of substrates. In the present study, an MFS protein CgMFS1 was characterized in C. gloeosporioides. This protein contains seven transmembrane domains, and its predicted 3D structure is hi...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Na Liu Qiannan Wang Chaozu He Bang An Source Type: research

< em > MC1R < /em > Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer
In conclusion, MC1R plays a crucial role in the progression of CRC and may be a marker of poor prognosis in CRC.PMID:34698109 | DOI:10.3390/cimb43030108 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Lixiong Peng Jiang Chang Xilin Liu Shiying Lu Honglin Ren Xiaoshi Zhou Zengshan Liu Pan Hu Source Type: research

AoP-LSE: Antioxidant Proteins Classification Using Deep Latent Space Encoding of Sequence Features
Curr Issues Mol Biol. 2021 Oct 9;43(3):1489-1501. doi: 10.3390/cimb43030105.ABSTRACTIt is of utmost importance to develop a computational method for accurate prediction of antioxidants, as they play a vital role in the prevention of several diseases caused by oxidative stress. In this correspondence, we present an effective computational methodology based on the notion of deep latent space encoding. A deep neural network classifier fused with an auto-encoder learns class labels in a pruned latent space. This strategy has eliminated the need to separately develop classifier and the feature selection model, allowing the stan...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Muhammad Usman Shujaat Khan Seongyong Park Jeong-A Lee Source Type: research

Genome-Wide Identification of LRR-RLK Family in < em > Saccharum < /em > and Expression Analysis in Response to Biotic and Abiotic Stress
Curr Issues Mol Biol. 2021 Oct 18;43(3):1632-1651. doi: 10.3390/cimb43030116.ABSTRACTThe leucine-rich repeat receptor-like protein kinase (LRR-RLK) gene family is the largest family of the receptor-like protein kinases (RLKs) superfamily in higher plants, which is involved in regulating the plant growth and development, stress responses, signal transduction and so on. However, no comprehensive analyses of LRR-RLKs have been reported in sugarcane. Here, we performed a comprehensive analysis of the LRR-RLK gene family in sugarcane ancestor species Saccharum spontaneum. A total of 437 LRR-RLK genes were identified and categor...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Wei Cheng Zhoutao Wang Fu Xu Waqar Ahmad Guilong Lu Yachun Su Liping Xu Source Type: research

Effects of Sample Size on Plant Single-Cell RNA Profiling
In this study, we evaluated the effects of sample size (i.e., cell number) on the outcome of single-cell transcriptome analysis by sampling different numbers of cells from a pool of ~57,000 Arabidopsis thaliana root cells integrated from five published studies. Our results indicated that the most significant principal components could be achieved when 20,000-30,000 cells were sampled, a relatively high reliability of cell clustering could be achieved by using ~20,000 cells with little further improvement by using more cells, 96% of the differentially expressed genes could be successfully identified with no more than 20,000...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Hongyu Chen Yang Lv Xinxin Yin Xi Chen Qinjie Chu Qian-Hao Zhu Longjiang Fan Longbiao Guo Source Type: research

FLII and MLL1 Cooperatively Regulate Aryl Hydrocarbon Receptor-Mediated Transcription in ARPE-19 Cells
In this study, we reveal that the histone methyltransferase MLL1 and the coactivator FLII are involved in AHR-mediated gene expression in retinal pigment epithelial cells. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) significantly increased the expression of CYP1A1, CYP1B1 and AHRR in ARPE-19 cells, whereas FLII or MLL1 depletion significantly reduced the expression of these genes induced by TCDD. Mechanistically, FLII binds to AHR in a ligand-dependent manner in ARPE-19 cells. In particular, the binding of FLII to MLL1 occurs through the GelB domain of FLII. In addition, MLL1 binds to AHR in a ligand-independent manner. FLI...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Kwang Won Jeong Source Type: research

Complete Mitochondrial Genomes of < em > Metcalfa pruinosa < /em > and < em > Salurnis marginella < /em > (Hemiptera: Flatidae): Genomic Comparison and Phylogenetic Inference in Fulgoroidea
Curr Issues Mol Biol. 2021 Sep 30;43(3):1391-1418. doi: 10.3390/cimb43030099.ABSTRACTThe complete mitochondrial genomes (mitogenomes) of two DNA barcode-defined haplotypes of Metcalfa pruinosa and one of Salurnis marginella (Hemiptera: Flatidae) were sequenced and compared to those of other Fulgoroidea species. Furthermore, the mitogenome sequences were used to reconstruct phylogenetic relationships among fulgoroid families. The three mitogenomes, including that of the available species of Flatidae, commonly possessed distinctive structures in the 1702-1836 bp A+T-rich region, such as two repeat regions at each end and a l...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Min Jee Kim Keon Hee Lee Jeong Sun Park Jun Seong Jeong Na Ra Jeong Wonhoon Lee Iksoo Kim Source Type: research

Diversification of Ferredoxins across Living Organisms
This study opened new vistas for further analysis of diversity of ferredoxins in living organisms.PMID:34698119 | DOI:10.3390/cimb43030098 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Nomfundo Nzuza Tiara Padayachee Wanping Chen Dominik Gront David R Nelson Khajamohiddin Syed Source Type: research

A Fast Image Alignment Approach for 2D Classification of Cryo-EM Images Using Spectral Clustering
Curr Issues Mol Biol. 2021 Oct 18;43(3):1652-1668. doi: 10.3390/cimb43030117.ABSTRACTThree-dimensional (3D) reconstruction in single-particle cryo-electron microscopy (cryo-EM) is a significant technique for recovering the 3D structure of proteins or other biological macromolecules from their two-dimensional (2D) noisy projection images taken from unknown random directions. Class averaging in single-particle cryo-EM is an important procedure for producing high-quality initial 3D structures, where image alignment is a fundamental step. In this paper, an efficient image alignment algorithm using 2D interpolation in the frequ...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Xiangwen Wang Yonggang Lu Jiaxuan Liu Source Type: research

Amelioration of Diabetic Nephropathy by Targeting Autophagy via Rapamycin or Fasting: Relation to Cell Apoptosis/Survival
In conclusion, this study provides a potential link between rapamycin or fasting for the cross-regulation of apoptosis and autophagy in the setting of cell stress as DN. Unlike rapamycin, fasting enhanced the active expression of ABCB1 efflux protein, providing insights on the potential ameliorative effects of fasting in DN that require further elucidation.PMID:34698133 | DOI:10.3390/cimb43030120 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Khaled Gouda Sherihan AbdelHamid Ahmed Mansour Nesreen Omar Hala El-Mesallamy Source Type: research

Oxidized Cell-Free DNA Rapidly Skews the Transcriptional Profile of Brain Cells toward Boosting Neurogenesis and Neuroplasticity
Curr Issues Mol Biol. 2021 Oct 13;43(3):1583-1591. doi: 10.3390/cimb43030112.ABSTRACTCell-free DNA (cfDNA) is liberated and accumulated in neural tissue due to cell damage. The oxidative and nitrosative stress in the brain that accompanies various pathological conditions has been shown to increase the oxidation of cellular and cell-free DNA. Whether the high concentration of non-oxidized and oxidized cfDNA may affect the transcriptome response of brain cells has not been studied. In the current work, we studied whether cfDNA fragments affect several key pathways, including neurogenesis, at the level of gene expression in b...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Anton D Filev Svetlana V Kostyuk Pavel E Umriukhin Vladimir M Pisarev Source Type: research

Modulation of the Physical Properties of 3D Spheroids Derived from Human Scleral Stroma Fibroblasts (HSSFs) with Different Axial Lengths Obtained from Surgical Patients
Curr Issues Mol Biol. 2021 Oct 22;43(3):1715-1725. doi: 10.3390/cimb43030121.ABSTRACTIn the current study, to elucidate the pathological characteristics of myopic scleral stroma, three-dimensional (3D) cultures of human scleral stroma fibroblasts (HSSFs) with several axial lengths (AL, 22.80-30.63 mm) that were obtained from patients (n = 7) were examined. Among the three groups of ALs, 30 mm (n = 3), the physical properties of the 3D HSSFs spheroids with respect to size and stiffness, the expressions of extracellular matrix (ECM) molecules, including collagen (COL) 1, 4, and 6 and fibronectin (FN) by qPCR and immunohistoc...
Source: Current Issues in Molecular Biology - October 26, 2021 Category: Molecular Biology Authors: Hiroyasu Katayama Masato Furuhashi Araya Umetsu Fumihito Hikage Megumi Watanabe Hiroshi Ohguro Yosuke Ida Source Type: research

Neonatal Diabetes in Patients Affected by Liang-Wang Syndrome Carrying KCNMA1 Variant p.(Gly375Arg) Suggest a Potential Role of Ca < sup > 2+ < /sup > and Voltage-Activated K < sup > + < /sup > Channel Activity in Human Insulin Secretion
We present the case of a newborn affected by LIWAS carrying the p.(Gly375Arg) variant who manifested diabetes in the first week of life. The description of our case strongly increases the frequency of ND in LIWAS patients and suggests a role of BK inactivation in human insulin secretion. The knowledge on the role of BKC in insulin secretion is very poor. Analyzing the possible mechanisms that could explain the association of LIWAS with ND, we speculate that BK inactivation might impair insulin secretion through the alteration of ion-dependent membrane activities and mitochondrial functions in β-cells, as well as the i...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Chiara Mameli Roberta Cazzola Luigina Spaccini Valeria Calcaterra Maddalena Macedoni Paola Azzurra La Verde Enza D'Auria Elvira Verduci Gianluca Lista Gian Vincenzo Zuccotti Source Type: research

Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers
Curr Issues Mol Biol. 2021 Sep 2;43(2):1043-1056. doi: 10.3390/cimb43020074.ABSTRACTColorectal cancer (CRC) is one of the leading causes of cancer-related death over the world. There is a great need for biomarkers capable of early detection and as targets for treatment. Differential protein expression was investigated with two-dimensional gel electrophoresis (2D-PAGE) followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in CRC patient tissue from (i) the peripheral part of the tumor, (ii) the central part of the tumor as well as from (iii) a non-involved part of the colorectal tissue. T...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Maja Ludvigsen Louise Thorlacius-Ussing Henrik Vorum Mogens Tornby Stender Ole Thorlacius-Ussing Bent Honor é Source Type: research

Resveratrol Ameliorates Aortic Calcification in Ovariectomized Rats via SIRT1 Signaling
In conclusion, the phytoestrogen resveratrol may be a safer alternative to estrogen, as a therapeutic approach against the progression of vascular calcification during postmenopause.PMID:34563044 | DOI:10.3390/cimb43020075 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Sally K Hammad Rana G Eissa Mohamed A Shaheen Nahla N Younis Source Type: research

Regulation of Immunity in Clear Cell Renal Carcinoma: Role of PD-1, PD-L1, and PD-L2
CONCLUSION: We present a new instrument targeting pathologies with p-VHL/HIF altered function that impact the PD-L2 expression through the change in transcriptional, growth factors, and AKT/mTOR modulation.PMID:34563045 | DOI:10.3390/cimb43020076 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Liudmila Spirina Zahar Yurmazov Evgeny Usynin Irina Kondakova Ekaterine Ladutko Evgeny Choynzonov Source Type: research

Hemopexin Modulates Expression of Complement Regulatory Proteins in Rat Glomeruli
Curr Issues Mol Biol. 2021 Sep 7;43(2):1081-1089. doi: 10.3390/cimb43020077.ABSTRACTIn systemic hemolysis and in hematuric forms of kidney injury, the major heme scavenging protein, hemopexin (HPX), becomes depleted, and the glomerular microvasculature (glomeruli) is exposed to high concentrations of unbound heme, which, in addition to causing oxidative injury, can activate complement cascades; thus, compounding extent of injury. It is unknown whether unbound heme can also activate specific complement regulatory proteins that could defend against complement-dependent injury. Isolated rat glomeruli were incubated in media s...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Maria G Detsika Elias A Lianos Source Type: research

Screening for Copy Number Variations of the 15q13.3 Hotspot in < em > CHRNA7 < /em > Gene and Expression in Patients with Migraines
Curr Issues Mol Biol. 2021 Sep 7;43(2):1090-1113. doi: 10.3390/cimb43020078.ABSTRACTBACKGROUND: a migraine is a neurological disease. Copy number variation (CNV) is a phenomenon in which parts of the genome are repeated. We investigated the effects of the CNV and gene expression at the location 15q13.3 in the Cholinergic Receptor Nicotinic Alpha 7 Subunit (CHRNA7) gene, which we believe to be effective in the migraine clinic.METHODS: we evaluated changes in CHRNA7 gene expression levels and CNV of 15q13.3 in patients with migraine (n = 102, with aura, n = 43; without aura, n = 59) according to healthy controls (n = 120) by...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Mehmet Fatih Özaltun S ırma Geyik Şenay Görücü Yılmaz Source Type: research

< em > Saussurea lappa < /em > Exhibits Anti-Oncogenic Effect in Hepatocellular Carcinoma, HepG2 Cancer Cell Line by Bcl-2 Mediated Apoptotic Pathway and Mitochondrial Cytochrome C Release
Conclusion: These findings conclude that S. lappa is a cause of hepatic cancer cell death through apoptosis and a potential natural source suggesting furthermore investigation of its active compounds that are responsible for these observed activities.PMID:34563048 | DOI:10.3390/cimb43020079 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Amal A Alotaibi Asmatanzeem Bepari Rasha Assad Assiri Shaik Kalimulla Niazi Sreenivasa Nayaka Muthuraj Rudrappa Shashiraj Kareyellapa Nagaraja Meghashyama Prabhakara Bhat Source Type: research

Chromosomal Location of Genes Differentially Expressed in Tumor Cells Surviving High-Dose X-ray Irradiation: A Preliminary Study on Radio-Fragile Sites
Curr Issues Mol Biol. 2021 Sep 8;43(2):1133-1141. doi: 10.3390/cimb43020080.ABSTRACTAltered gene expression is a common feature of tumor cells after irradiation. Our previous study showed that this phenomenon is not only an acute response to cytotoxic stress, instead, it was persistently detected in tumor cells that survived 10 Gy irradiation (IR cells). The current understanding is that DNA double-strand breaks (DSBs) are recognized by the phosphorylation of histone H2AX (H2AX) and triggers the ataxia-telangiectasia mutated (ATM) protein or the ATM- and Rad3-related (ATR) pathway, which activate or inactivate the DNA repa...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Kaori Tsutsumi Moe Masuda Hiroyuki Date Source Type: research

Combined Transcriptome and Proteome Analysis of Maize ( < em > Zea mays < /em > L.) Reveals A Complementary Profile in Response to Phosphate Deficiency
Curr Issues Mol Biol. 2021 Sep 13;43(2):1142-1155. doi: 10.3390/cimb43020081.ABSTRACTA deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequ...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Zhi Nie Bowen Luo Xiao Zhang Ling Wu Dan Liu Jialei Guo Xuan He Duojiang Gao Shiqiang Gao Shibin Gao Source Type: research

HPRep: Quantifying Reproducibility in HiChIP and PLAC-Seq Datasets
Curr Issues Mol Biol. 2021 Sep 17;43(2):1156-1170. doi: 10.3390/cimb43020082.ABSTRACTHiChIP and PLAC-Seq are emerging technologies for studying genome-wide long-range chromatin interactions mediated by the protein of interest, enabling more sensitive and cost-efficient interrogation of protein-centric chromatin conformation. However, due to the unbalanced read distribution introduced by protein immunoprecipitation, existing reproducibility measures developed for Hi-C data are not appropriate for the analysis of HiChIP and PLAC-Seq data. Here, we present HPRep, a stratified and weighted correlation metric derived from norma...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Jonathan D Rosen Yuchen Yang Armen Abnousi Jiawen Chen Michael Song Ian R Jones Yin Shen Ming Hu Yun Li Source Type: research

Antioxidant and Anti-Melanogenic Activities of Heat-Treated Licorice (Wongam, < em > Glycyrrhiza glabra < /em > × < em > G. uralensis < /em > ) Extract
In conclusion, WH-130, with increased levels of bioactive phenolics such as isoliquiritigenin, has potential for development into a novel skin whitening material with applications in cosmetics.PMID:34563052 | DOI:10.3390/cimb43020083 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Min Hye Kang Gwi Yeong Jang Yun-Jeong Ji Jeong Hoon Lee Su Ji Choi Tae Kyung Hyun Hyung Don Kim Source Type: research

Oncological Properties of Intravenous Leiomyomatosis: Involvement of Mesenchymal Tumor Stem-Like Cells
We examined the oncological properties of intravenous leiomyomatosis using molecular pathological techniques on tissue excised from patients with uterine leiomyoma. CD44-positive mesenchymal tumor stem-like cells were detected in both patients with intravenous leiomyomatosis and uterine leiomyosarcoma. The oncological properties of intravenous leiomyomatosis were found to be similar to those of uterine leiomyosarcoma. However, in intravenous leiomyomatosis, cyclin E and Ki-67-positive cells were rare and no pathological findings suspecting malignancy were observed. It is expected that establishing a treatment method target...
Source: Current Issues in Molecular Biology - September 25, 2021 Category: Molecular Biology Authors: Saya Tamura Takuma Hayashi Hideki Tokunaga Nobuo Yaegashi Kaoru Abiko Ikuo Konishi Source Type: research