Single-Cell RNA Sequencing Reveals Alterations in Patient Immune Cells with Pulmonary Long COVID-19 Complications
This study aims to delve deeper into the immune landscape of patients with prolonged symptoms by implementing single-cell mRNA analysis. A 71-year-old COVID-19 patient presenting with persistent viral pneumonia was recruited, and peripheral blood samples were taken at 3 and 2 years post-acute infection onset. Patients and control peripheral blood mononuclear cells (PBMCs) were isolated and single-cell sequenced. Immune cell population identification was carried out using the ScType script. Three months post-COVID-19 patients' PBMCs contained a significantly larger immature neutrophil population compared to 2-year and contr...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Krist īne Vaivode Rihards Saksis Hel ēna Daiga Litvina Helvijs Niedra Marta L īva Spriņģe Una Kr ūmiņa J ānis Kloviņš Vita Rovite Source Type: research

Molecular Foundations of Inflammatory Diseases: Insights into Inflammation and Inflammasomes
Curr Issues Mol Biol. 2024 Jan 3;46(1):469-484. doi: 10.3390/cimb46010030.ABSTRACTInflammatory diseases are a global health problem affecting millions of people with a wide range of conditions. These diseases, including inflammatory bowel disease (IBD), rheumatoid arthritis (RA), osteoarthritis (OA), gout, and diabetes, impose a significant burden on patients and healthcare systems. A complicated interaction between genetic variables, environmental stimuli, and dysregulated immune responses shows the complex biological foundation of various diseases. This review focuses on the molecular mechanisms underlying inflammatory d...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Mi Eun Kim Jun Sik Lee Source Type: research

Investigating Cox-2 and EGFR as Biomarkers in Canine Oral Squamous Cell Carcinoma: Implications for Diagnosis and Therapy
In this study, we aimed to investigate the significance of two key molecular components, Cox-2 and EGFR, in canine OSCC. We examined 34 tumor sections from various dog breeds to assess the immunoexpression of Cox-2 and EGFR. Our findings revealed that Cox-2 was highly expressed in 70.6% of cases, while EGFR overexpression was observed in 44.1%. Cox-2 overexpression showed association with histological grade of malignancy (HGM) (p = 0.006) and EGFR with vascular invasion (p = 0.006). COX-2 and EGFR concurrent expression was associated with HGM (p = 0.002), as well as with the presence of vascular invasion (p = 0.002). These...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Rita Files Catarina Santos Felisbina L Queiroga Filipe Silva Leonor Delgado Isabel Pires Justina Prada Source Type: research

Rapid Visual Detection of Elite Erect Panicle < em > Dense and Erect Panicle 1 < /em > Allele for Marker-Assisted Improvement in Rice ( < em > Oryza sativa < /em > L.) Using the Loop-Mediated Isothermal Amplification Method
In this study, a colorimetric LAMP (loop-mediated isothermal amplification) assay was developed for the detection of the dep1 allele and tested for the screening and selection of the heavy-panicle hybrid rice elite restorer line SHUHUI498, modified with the allele. InDel (Insertion and Deletion) primers (DEP1_F and DEP1_R) and LAMP primers (F3, B3, FIP, and BIP) for genotyping were designed using the Primer3 Plus (version 3.3.0) and PrimerExplore (version 5) software. Our results showed that both InDel and LAMP markers could be used for accurate genotyping. After incubation at a constant temperature of 65 °C for 60 min wi...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Yonghang Tian Xiyi Chen Peizhou Xu Yuping Wang Xianjun Wu Kun Wu Xiangdong Fu Yaoxian Chin Yongxiang Liao Source Type: research

Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of < em > Lactobacillus iners < /em > Derived from Korean Women's Skin in Their Twenties
Curr Issues Mol Biol. 2024 Jan 4;46(1):513-526. doi: 10.3390/cimb46010033.ABSTRACTThe process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic aging, mainly caused by exposure to UV radiation, resulting in photoaging. Photoaging manifests as skin issues such as wrinkles and discoloration. The skin microbiome, a diverse community of microorganisms on the skin's surface, plays a crucial role in skin protection and can be affected by factors like humidity and pH. Probiotics, beneficial microorganisms, have been investigated for their potential to enhance skin health by regul...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Jin-Sung Lee Jin-Woo Min Seong-Bong Gye Yong-Woo Kim Hee-Cheol Kang Yoon-Seo Choi Won-Sang Seo Bun-Yeoul Lee Source Type: research

Cytotoxic Effect of < em > Andrographis paniculata < /em > Associated with 2-Aminoethyl Dihydrogen Phosphate in Triple-Negative Breast Cells
This study aims to assess the antitumor, antiproliferative, and pharmacological effects of andrographolide at different concentrations, both individually and in conjunction with 2-aminoethyl dihydrogen phosphate. The cytotoxicity of the treatments was evaluated using the colorimetric MTT method, cell cycle phases, mitochondrial electrical potential, and markers expression via flow cytometry, while the pharmacological effects were assessed using SynergyFinder software 3.0. Treatments with A. paniculata, isolated at concentrations of 10%, 30%, and 50% of andrographolide, induced cell death in tumor cells, resulting in a redu...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Rosa Andrea Nogueira Laiso Julia Carolina Ferreira Rose Eli Grassi Rici Laertty Garcia de Sousa Cabral Durvanei Augusto Maria Source Type: research

Production of Recombinant Single-Chain Eel Luteinizing Hormone and Follicle-Stimulating Hormone Analogs in Chinese Hamster Ovary Suspension Cell Culture
Curr Issues Mol Biol. 2024 Jan 5;46(1):542-556. doi: 10.3390/cimb46010035.ABSTRACTWe produced rec-single chain eel luteinizing (rec-eel LH) and follicle-stimulating (rec- eel FSH) hormones displaying high biological activity in Chinese hamster ovary suspension (CHO-S) cells. We constructed several mutants, in which a linker, including an O-linked glycosylated carboxyl-terminal peptide (CTP) of an equine chorionic gonadotropin (eCG) β-subunit, was attached between the β- and α-subunit (LH-M and FSH-M) or in the N-terminal (C-LH and C-FSH) or C-terminal (LH-C and FSH-C) regions. The plasmids were transfected into CHO-S ce...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Munkhzaya Byambaragchaa Sang-Gwon Kim Sei Hyun Park Min Gyu Shin Shin-Kwon Kim Myung-Hwa Kang Kwan-Sik Min Source Type: research

AG1 < sup > ® < /sup > Induces a Favorable Impact on Gut Microbial Structure and Functionality in the Simulator of Human Intestinal Microbial Ecosystem < sup > ® < /sup > Model
Curr Issues Mol Biol. 2024 Jan 5;46(1):557-569. doi: 10.3390/cimb46010036.ABSTRACTModulation of the human gut microbiome has become an area of interest in the nutraceutical space. We explored the effect of the novel foundational nutrition supplement AG1® on the composition of human microbiota in an in vitro experimental design. Employing the Simulator of Human Intestinal Microbial Ecosystem (SHIME®) model, AG1® underwent digestion, absorption, and subsequent colonic microenvironment simulation under physiologically relevant conditions in healthy human fecal inocula. Following 48 h of colonic simulation, the gut microbio...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Trevor O Kirby Philip A Sapp Jeremy R Townsend Marlies Govaert Cindy Duysburgh Massimo Marzorati Tess M Marshall Ralph Esposito Source Type: research

The Development of a CRISPR-FnCpf1 System for Large-Fragment Deletion and Multiplex Gene Editing in < em > Acinetobacter baumannii < /em >
In this study, we report a new CRISPR-FnCpf1-based two-plasmid system for versatile and precise genome editing in A. baumannii. After identification, this new system prefers to recognize the 5'-TTN-3' (N = A, T, C or G) and the 5'-CTV-3' (V = A, C or G) protospacer-adjacent motif (PAM) sequence and utilize the spacer with lengths ranging from 19 to 25 nt. In direct comparison with the existing CRISPR-Cas9 system, it exhibits approximately four times the targetable range in A. baumannii. Moreover, by employing a tandem dual crRNA expression cassette, the new system can perform large-fragment deletion and simultaneous multip...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Shuai Wang Yue Ding Hua Rong Yu Wang Source Type: research

Harnessing Natural Polymers for Nano-Scaffolds in Bone Tissue Engineering: A Comprehensive Overview of Bone Disease Treatment
Curr Issues Mol Biol. 2024 Jan 5;46(1):585-611. doi: 10.3390/cimb46010038.ABSTRACTNumerous surgeries are carried out to replace tissues that have been harmed by an illness or an accident. Due to various surgical interventions and the requirement of bone substitutes, the emerging field of bone tissue engineering attempts to repair damaged tissues with the help of scaffolds. These scaffolds act as template for bone regeneration by controlling the development of new cells. For the creation of functional tissues and organs, there are three elements of bone tissue engineering that play very crucial role: cells, signals and scaf...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Sushmita Saurav Prashish Sharma Anil Kumar Zeba Tabassum Madhuri Girdhar Narsimha Mamidi Anand Mohan Source Type: research

Comparison of Retinal Metabolic Activity and Structural Development between rd10 Mice and Normal Mice Using Multiphoton Fluorescence Lifetime Imaging Microscopy
Curr Issues Mol Biol. 2024 Jan 6;46(1):612-620. doi: 10.3390/cimb46010039.ABSTRACTFluorescence lifetime imaging microscopy (FLIM) is a technique that analyzes the metabolic state of tissues based on the spatial distribution of fluorescence lifetimes of certain interacting molecules. We used multiphoton FLIM to study the metabolic state of developing C57BL6/J and rd10 retinas based on the fluorescence lifetimes of free versus bound nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate (NAD(P)H), with free NAD(P)H percentages suggesting increased glycolysis and bound NAD(P)H percentages indicating...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Erin Su Niranjana Kesavamoorthy Jason A Junge Mengmei Zheng Cheryl Mae Craft Hossein Ameri Source Type: research

Sodium Dodecyl Sulfate Analogs as a Potential Molecular Biology Reagent
In this study, we review the properties of three anionic detergents, sodium dodecyl sulfate (SDS), Sarkosyl, and sodium lauroylglutamate (SLG), as they play a critical role in molecular biology research. SDS is widely used in electrophoresis and cell lysis for proteomics. Sarkosyl and, more frequently, SDS are used for the characterization of neuropathological protein fibrils and the solubilization of proteins. Many amyloid fibrils are resistant to SDS or Sarkosyl to different degrees and, thus, can be readily isolated from detergent-sensitive proteins. SLG is milder than the above two detergents and has been used in the s...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Tsutomu Arakawa Takako Niikura Yoshiko Kita Teruo Akuta Source Type: research

Phosphorylation Codes in IRS-1 and IRS-2 Are Associated with the Activation/Inhibition of Insulin Canonical Signaling Pathways
Curr Issues Mol Biol. 2024 Jan 9;46(1):634-649. doi: 10.3390/cimb46010041.ABSTRACTInsulin receptor substrates 1 and 2 (IRS-1 and IRS-2) are signaling adaptor proteins that participate in canonical pathways, where insulin cascade activation occurs, as well as in non-canonical pathways, in which phosphorylation of substrates is carried out by a diverse array of receptors including integrins, cytokines, steroid hormones, and others. IRS proteins are subject to a spectrum of post-translational modifications essential for their activation, encompassing phosphorylation events in distinct tyrosine, serine, and threonine residues....
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Anabel Mart ínez Báez Guadalupe Ayala Adolfo Pedroza-Saavedra Hilda M Gonz ález-Sánchez Lilia Chihu Amparan Source Type: research

Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells
Curr Issues Mol Biol. 2024 Jan 9;46(1):650-662. doi: 10.3390/cimb46010042.ABSTRACTIsocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechanical properties of ICD in OSCC were determined. Changes in the contents of reactive oxygen species (ROS), the mitochondrial membrane potential (MMP), ATP, and mitochondrial respiratory chain complex enzymes Ⅰ-Ⅳ in cancer cells were studied. ICD ...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Qiaozhen Zhou Qianqian Zhang Lingzi Liao Qian Li Huidan Qu Xinyu Wang Ying Zhou Guangzeng Zhang Mingliang Sun Kailiang Zhang Baoping Zhang Source Type: research

Pathological and Therapeutical Implications of Pyroptosis in Psoriasis and Hidradenitis Suppurativa: A Narrative Review
This study delves into the potential therapeutic implications of targeting pyroptosis in psoriasis and HS, examining existing medications like biologics and Janus kinase inhibitors. It also reviews the current limitations and challenges in developing therapies that selectively target pyroptosis. Additionally, the manuscript explores the role of pyroptosis in various inflammatory disorders associated with psoriasis and HS, such as inflammatory bowel disease, diabetes mellitus, and cardiovascular disorders. The review concludes by emphasizing the need for further research to fully elucidate the pathomechanisms of these derma...
Source: Current Issues in Molecular Biology - January 22, 2024 Category: Molecular Biology Authors: Piotr K Krajewski Maria Tsoukas Jacek C Szepietowski Source Type: research