Coffee and Microbiota: A Narrative Review
Curr Issues Mol Biol. 2024 Jan 20;46(1):896-908. doi: 10.3390/cimb46010057.ABSTRACTCoffee is one of the most widely consumed beverages in the world, which has important repercussions on the health of the individual, mainly because of certain compounds it contains. Coffee consumption exerts significant influences on the entire body, including the gastrointestinal tract, where a central role is played by the gut microbiota. Dysbiosis in the gut microbiota is implicated in the occurrence of numerous diseases, and knowledge of the microbiota has proven to be of fundamental importance for the development of new therapeutic stra...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Federico Rosa Benedetta Marigliano Sergio Mannucci Marcello Candelli Gabriele Savioli Giuseppe Merra Maurizio Gabrielli Antonio Gasbarrini Francesco Franceschi Andrea Piccioni Source Type: research

In Vitro Anti-Inflammatory Study of Limonoids Isolated from < em > Chisocheton < /em > Plants
In this report, we evaluated the anti-inflammatory activity of 17 limonoid compounds from Chisocheton plant primarily by measuring their inhibitory effects on the production of pro-inflammatory cytokines, including TNF-α, IL-6, IL-1β, and MCP-1, in LPS-stimulated THP-1 cells using an ELISA assay. Compounds 3, 5, 9, and 14-17 exhibited significant activity in inhibiting the evaluated pro-inflammatory markers, with IC50 values less than 20 µM and a high selectivity index (SI) range. Compounds 3, 5, 9, and 15 significantly suppressed the expression of phosphorylated p38 MAPK in THP-1 cells stimulated with LPS. These findin...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Erina Hilmayanti Xuhao Huang Supriatno Salam None Nurlelasari Unang Supratman Kazuya Kabayama Koichi Fukase Source Type: research

Evaluation of the Reparative Effect of Sinomenine in an Acetaminophen-Induced Liver Injury Model
This study suggests that SIN may treat acute liver damage, warranting further research into its long-term effects, optimal dosage, and clinical applications. These findings aid liver-related emergency department interventions and life-saving treatments.PMID:38275673 | PMC:PMC10814253 | DOI:10.3390/cimb46010059 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Ahmet Kayal ı Ejder Saylav Bora H üseyin Acar Oytun Erba ş Source Type: research

Genetic Predisposition for White Matter Hyperintensities and Risk of Mild Cognitive Impairment and Alzheimer's Disease: Results from the HELIAD Study
Curr Issues Mol Biol. 2024 Jan 22;46(1):934-947. doi: 10.3390/cimb46010060.ABSTRACTThe present study investigated the association of genetic predisposition for white matter hyperintensities (WMHs) with incident amnestic mild cognitive impairment (aMCI) or Alzheimer's disease (AD), as well as whether such an association was influenced by age, sex, and cognitive reserve. Overall, 537 individuals without aMCI or dementia at baseline were included. Among them, 62 individuals developed aMCI/AD at follow up. Genetic propensity to WMH was estimated using a polygenic risk score for WMHs (PRS WMH). The association of PRS WMH with a...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Stefanos N Sampatakakis Niki Mourtzi Sokratis Charisis Eirini Mamalaki Eva Ntanasi Alexandros Hatzimanolis Alfredo Ramirez Jean-Charles Lambert Mary Yannakoulia Mary H Kosmidis Efthimios Dardiotis Georgios Hadjigeorgiou Paraskevi Sakka Nikolaos Scarmeas Source Type: research

Ghrelin in Focus: Dissecting Its Critical Roles in Gastrointestinal Pathologies and Therapies
This article accentuates ghrelin's multifunctionality, shedding light on its influence on disease mechanisms, including inflammatory responses and cancer progression, and examines its therapeutic potential in GI surgeries and disorders, advocating for continued research in this evolving field.PMID:38275675 | PMC:PMC10813987 | DOI:10.3390/cimb46010061 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Wei Wu Lei Zhu Zhimin Dou Qiliang Hou Sen Wang Ziqian Yuan Bin Li Source Type: research

Gallic Acid Protects from Sepsis-Induced Acute Lung Injury
This study aims to observe gallic acid's protective and restorative effect on the lungs in an experimental sepsis model. Male Wistar albino rats were subjected to a feces intraperitoneal injection procedure (FIP) to induce sepsis. Four groups were formed: normal control, FIP alone, FIP with saline, and FIP with gallic acid. Gallic acid was administered intraperitoneally at 20 mg/kg/day. Blood samples were collected for biochemical analysis, and computed tomography assessed lung tissue histopathologically and radiologically. Gallic acid significantly decreased malondialdehyde, IL-6, IL-1β, TNF-α, CRP levels, oxidative str...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: S üleyman Kardaş Osman Sezer Çınaroğlu Ejder Saylav Bora Oytun Erba ş Source Type: research

Global Analysis of the WOX Transcription Factor Family in < em > Akebia trifoliata < /em >
Curr Issues Mol Biol. 2023 Dec 19;46(1):11-24. doi: 10.3390/cimb46010002.ABSTRACTAkebia trifoliata is an economically important, self-incompatible fruit tree in the Lardizabalaceae family. Asexual propagation is the main strategy used to maintain excellent agronomic traits. However, the generation of adventitious roots during asexual propagation is very difficult. To study the important role of the WUSCHEL-related homeobox (WOX) transcription factor in adventitious root growth and development, we characterized this transcription factor family in the whole genome of A. trifoliata. A total of 10 AktWOXs were identified, with...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Shengpeng Chen Huai Yang Yongle Zhang Chen Chen Tianheng Ren Feiquan Tan Peigao Luo Source Type: research

Excess BAFF May Impact HIV-1-Specific Antibodies and May Promote Polyclonal Responses Including Those from First-Line Marginal Zone B-Cell Populations
Curr Issues Mol Biol. 2023 Dec 19;46(1):25-43. doi: 10.3390/cimb46010003.ABSTRACTWe have previously shown that blood levels of B-cell Activating Factor (BAFF) rise relatively to disease progression status in the context of HIV-1 infection. Excess BAFF was concomitant with hyperglobulinemia and the deregulation of blood B-cell populations, notably with increased frequencies of a population sharing characteristics of transitional immature and marginal zone (MZ) B-cells, which we defined as marginal zone precursor-like" (MZp). In HIV-uninfected individuals, MZp present a B-cell regulatory (Breg) profile and function, which ar...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Kim Doyon-Lalibert é Matheus Aranguren Josiane Chagnon-Choquet Laurie-Anne Batraville Olina Dagher Jonathan Richard Matteo Paniconi Jean-Pierre Routy C écile Tremblay Marie-Claude Quintal Nathalie Brassard Daniel E Kaufmann Andr és Finzi Johanne Poudri Source Type: research

Anti-Prokineticin1 Suppresses Liver Metastatic Tumors in a Mouse Model of Colorectal Cancer with Liver Metastasis
In this study, a liver metastasis mouse model was developed using human colorectal cancer cell lines, and mice were divided into anti-PROK1 antibody administration and control groups. Mice were treated intraperitoneally with antibodies or phosphate-buffered saline (control) every three days. The number, size, and cell proliferation ability of metastatic lesions were analyzed. Our results suggested that the number, size, and cancer cell proliferation ability of metastatic lesions decreased, and the survival time significantly increased in the antibody-treated group compared to those in the control group. Thus, the anti-PROK...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Hiroko Kono Takanori Goi Takayuki Matsunaka Kenji Koneri Source Type: research

Multiplex Microarrays in 96-Well Plates Photoactivated with 4-Azidotetrafluorobenzaldehyde for the Identification and Quantification of β-Lactamase Genes and Their RNA Transcripts
Curr Issues Mol Biol. 2023 Dec 20;46(1):53-66. doi: 10.3390/cimb46010005.ABSTRACTAntibiotic-resistant bacteria represent a global issue that calls for novel approaches to diagnosis and treatment. Given the variety of genetic factors that determine resistance, multiplex methods hold promise in this area. We developed a novel method to covalently attach oligonucleotide probes to the wells of polystyrene plates using photoactivation with 4-azidotetrafluorobenzaldehyde. Then, it was used to develop the technique of microarrays in the wells. It consists of the following steps: activating polystyrene, hybridizing the probes with...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Mariya M Ulyashova Galina V Presnova Anna A Filippova Vitaly G Grigorenko Alexey M Egorov Maya Yu Rubtsova Source Type: research

Arsenic Trioxide Decreases Lymphangiogenesis by Inducing Apoptotic Pathways and Inhibition of Important Endothelial Cell Receptors
In conclusion, we demonstrate that ATO inhibits lymphangiogenesis by activating apoptotic pathways and inhibiting important endothelial cell receptors, which suggests that this drug should be further evaluated in the treatment of tumor-associated lymphangiogenesis.PMID:38275666 | PMC:PMC10813910 | DOI:10.3390/cimb46010006 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Igor Hrgovic Eva Z öller Monika Doll Tsige Hailemariam-Jahn Thilo Jakob Roland Kaufmann Markus Meissner Johannes Kleemann Source Type: research

Insights into the Effects of Hydroxycinnamic Acid and Its Secondary Metabolites as Antioxidants for Oxidative Stress and Plant Growth under Environmental Stresses
Curr Issues Mol Biol. 2023 Dec 21;46(1):81-95. doi: 10.3390/cimb46010007.ABSTRACTPlant immobility renders plants constantly susceptible to various abiotic and biotic stresses. Abiotic and biotic stresses are known to produce reactive oxygen species (ROS), which cause comparable cellular secondary reactions (osmotic or oxidative stress), leading to agricultural productivity constraints worldwide. To mitigate the challenges caused by these stresses, plants have evolved a variety of adaptive strategies. Phenolic acids form a key component of these strategies, as they are predominantly known to be secreted by plants in respons...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Sindiswa Khawula Arun Gokul Lee-Ann Niekerk Gerhard Basson Marshall Keyster Mihlali Badiwe Ashwil Klein Mbukeni Nkomo Source Type: research

Inflammation and Starvation Affect Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells
In this report, the reliability of five commonly used HKGs (ACTB, B2M, GAPDH, HPRT1 and RPLP0) was tested in ASCs cultured under standard protocol after inflammatory priming or starvation. Gene expression data were computed with four different applets able to rank genes depending on their stability in either single or combined conditions. The obtained final ranking suggests that for each treatment, a specific HKG is needed, and that starvation is the condition with the stronger effect on HKGs' stability and, therefore, reliability. The normalization effect of proper HKGs' use was then validated on three genes involved in O...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Enrico Ragni Simona Piccolo Michela Taiana Caterina Visconte Giulio Grieco Laura de Girolamo Source Type: research

Methoxyfuranocoumarins of Natural Origin-Updating Biological Activity Research and Searching for New Directions-A Review
Curr Issues Mol Biol. 2024 Jan 19;46(1):856-883. doi: 10.3390/cimb46010055.ABSTRACTPlant secondary metabolites, including furanocoumarins, have attracted attention for decades as active molecules with therapeutic potential, especially those occurring in a limited number of species as evolutionarily specific and chemotaxonomically important. The most famous methoxyfuranocoumarins (MFCs), bergapten, xanthotoxin, isopimpinellin, phellopterin, byakangelicol, byakangelicin, isobergapten, pimpinellin, sphondin, as well as rare ones such as peucedanin and 8-methoxypeucedanin, apaensin, cnidilin, moellendorffiline and dahuribiethr...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Magdalena Bartnik Source Type: research

Anti-Neuroinflammatory Effects of < em > Arecae pericarpium < /em > on LPS-Stimulated BV2 Cells
Curr Issues Mol Biol. 2024 Jan 19;46(1):884-895. doi: 10.3390/cimb46010056.ABSTRACTArecae pericarpium (AP), the fruit peel of the betel palm, is a traditional Oriental herbal medicine. AP is used to treat various diseases and conditions, such as ascites, edema, and urinary retention, in traditional Korean medicine. Recent studies have demonstrated its anti-obesity and antibacterial effects; however, its anti-neuroinflammatory effects have not yet been reported. Therefore, we investigated the anti-neuroinflammatory effects of AP on lipopolysaccharide (LPS)-stimulated mouse microglia in this study. To determine the anti-neur...
Source: Current Issues in Molecular Biology - January 26, 2024 Category: Molecular Biology Authors: Hyeon-Gyu Cho Dong-Uk Kim Jin-Young Oh Sung-Joo Park Bitna Kweon Gi-Sang Bae Source Type: research