Nerve Growth Factor and the Role of Inflammation in Tumor Development
This report aims to provide a summary and subsequent review of the literature on the role of NGF in regulating the inflammatory microenvironment and tumor cell growth, survival, and death. The role of NGF in inflammation and tumorigenesis as a component of the inflammatory system, its interaction with the various components of the respective microenvironments, its ability to cause epigenetic changes, and its role in the treatment of cancer have been highlighted in this paper.PMID:38392180 | PMC:PMC10888178 | DOI:10.3390/cimb46020062 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Giampiero Ferraguti Sergio Terracina Luigi Tarani Francesca Fanfarillo Sara Allushi Brunella Caronti Paola Tirassa Antonella Polimeni Marco Lucarelli Luca Cavalcanti Antonio Greco Marco Fiore Source Type: research

Marine Bioactive Peptides: Anti-Photoaging Mechanisms and Potential Skin Protective Effects
Curr Issues Mol Biol. 2024 Jan 23;46(2):990-1009. doi: 10.3390/cimb46020063.ABSTRACTSkin photoaging, resulting from prolonged exposure to ultraviolet radiation, is a form of exogenous aging that not only impacts the aesthetic aspect of the skin but also exhibits a strong correlation with the onset of skin cancer. Nonetheless, the safety profile of non-natural anti-photoaging medications and the underlying physiological alterations during the process of photoaging remain inadequately elucidated. Consequently, there exists a pressing necessity to devise more secure interventions involving anti-photoaging drugs. Multiple stud...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Xiaoliang Zhang Hong Zhuang Sijia Wu Chen Mao Yaxi Dai Haiyang Yan Source Type: research

Association between Single Nucleotide Polymorphisms in Monoamine Oxidase and the Severity of Addiction to Betel Quid
Curr Issues Mol Biol. 2024 Jan 23;46(2):1010-1019. doi: 10.3390/cimb46020064.ABSTRACTBetel quid (BQ) is the fourth most popular psychoactive substance in the world, and BQ use disorder (BUD) is prevalent in Asian countries. Although the mechanisms underlying BUD remain unclear, studies have reported influences from monoamine oxidase inhibitor. We enrolled 50 patients with BUD and assessed their BQ consumption habits, emotional conditions, and the clinical severity of addiction-assessed using the Diagnostic and Statistical Manual of Mental Disorders [Fifth Edition] (DSM-5) criteria, Substance Use Severity Rating Scale, and ...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Chung-Chieh Hung Ying-Chin Ko Chia-Min Chung Source Type: research

Impact of Lysine Succinylation on the Biology of Fungi
Curr Issues Mol Biol. 2024 Jan 23;46(2):1020-1046. doi: 10.3390/cimb46020065.ABSTRACTPost-translational modifications (PTMs) play a crucial role in protein functionality and the control of various cellular processes and secondary metabolites (SMs) in fungi. Lysine succinylation (Ksuc) is an emerging protein PTM characterized by the addition of a succinyl group to a lysine residue, which induces substantial alteration in the chemical and structural properties of the affected protein. This chemical alteration is reversible, dynamic in nature, and evolutionarily conserved. Recent investigations of numerous proteins that under...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: John Adejor Elisabeth Tumukunde Guoqi Li Hong Lin Rui Xie Shihua Wang Source Type: research

An Expeditious Neutralization Assay for Porcine Reproductive and Respiratory Syndrome Virus Based on a Recombinant Virus Expressing Green Fluorescent Protein
Curr Issues Mol Biol. 2024 Jan 23;46(2):1047-1063. doi: 10.3390/cimb46020066.ABSTRACTDue to the extensive genetic and antigenic variation in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), as well as its rapid mutability and evolution, PRRS prevention and control can be challenging. An expeditious and sensitive neutralization assay for PRRSV is presented to monitor neutralizing antibodies (NAbs) in serum during vaccine research. Here, a PRRSV expressing eGFP was successfully rescued with reverse genetics based on the infectious clone HuN4-F112-eGFP which we constructed. The fluorescent protein expressions of t...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Juan Wang Jiecong Yan Shuaiyong Wang Ronglin Chen Yanru Xing Qingyan Liu Shuolei Gao Yuxiang Zhu Jiannan Li Yanjun Zhou Tongling Shan Wu Tong Hao Zheng Ning Kong Yifeng Jiang Changlong Liu Guangzhi Tong Hai Yu Source Type: research

Molecular Analysis of HLA Genes in Romanian Patients with Chronic Hepatitis B Virus Infection
This study's main findings include the identification of certain HLA alleles, such as DQB1*06:03:01, DRB1*13:01:01, DQB1*06:02:01, DQA1*01:03:01, DRB5*01:01:01, and DRB1*15:01:01, which exhibit a significant protective effect against HBV. Additionally, the amino acid residue alanine at DQB1_38 is associated with a protective role, while valine presence may signal an increased risk of hepatitis B. The present findings are important in addressing the urgent need for improved methods of diagnosing and managing CHB, particularly when considering the disease's presence in diverse population groups and geographical regions.PMID:...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Adriana T ălăngescu Bogdan Calenic Dan Florin Mih ăilescu Maria Tizu Ion Marun țelu Alexandra E Constantinescu Ileana Constantinescu Source Type: research

Antidiabetic Effect of Urolithin A in Cultured L6 Myotubes and Type 2 Diabetic Model KK-A < sup > y < /sup > /Ta Mice with Glucose Intolerance
Curr Issues Mol Biol. 2024 Jan 24;46(2):1078-1090. doi: 10.3390/cimb46020068.ABSTRACTDiabetes is caused by abnormal glucose metabolism, and muscle, the largest tissue in the human body, is largely involved. Urolithin A (UroA) is a major intestinal and microbial metabolite of ellagic acid and ellagitannins and is found in fruits such as strawberry and pomegranate. In this present study, we investigated the antidiabetic effects of UroA in L6 myotubes and in KK-Ay/Ta, a mouse model of type 2 diabetes (T2D). UroA treatment elevated the glucose uptake (GU) of L6 myotubes in the absence of insulin. This elevation in GU by UroA t...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Shinji Kondo Shin-Ichi Adachi Wataru Komatsu Fumiaki Yoshizawa Kazumi Yagasaki Source Type: research

Comparative Phylogenetic Analysis of Ancient Korean Tea "Hadong Cheon-Nyeon Cha ( < em > Camellia sinensis < /em > var. < em > sinensis < /em > )" Using Complete Chloroplast Genome Sequences
This study determined seven Camellia sinensis var. sinenesis, including Hadong Cheon-nyeon Cha (HCNC) chloroplast genome sequences, using Illumina sequencing technology via de novo assembly. The chloroplast genome sizes ranged from 157,019 to 157,114 bp and were organized into quadripartite regions with the typical chloroplast genomes. Further, differences in SNPs and InDels were detected across the seven chloroplast genomes through variance analysis. Principal component and phylogenetic analysis suggested that regional constraints, rather than functional constraints, strongly affected the sequence evolution of the cp geno...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Doobo Shim Seung Ho Jeon Jong Cheol Kim Dong-Kyung Yoon Source Type: research

< em > OCT4 < /em > Expression in Gliomas Is Dependent on Cell Metabolism
Curr Issues Mol Biol. 2024 Jan 25;46(2):1107-1120. doi: 10.3390/cimb46020070.ABSTRACTThe OCT4 transcription factor is necessary to maintain cell stemness in the early stages of embryogenesis and is involved in the formation of induced pluripotent stem cells, but its role in oncogenesis is not yet entirely clear. In this work, OCT4 expression was investigated in malignant gliomas. Twenty glioma cell lines and a sample of normal adult brain tissue were used. OCT4 expression was found in all studied glioma cell lines but was not detected in normal adult brain tissue. For one of these lines, OCT4 knockdown caused tumor cell de...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Andrey Volnitskiy Konstantin Shabalin Rimma Pantina Elena Varfolomeeva Roman Kovalev Vladimir Burdakov Svetlana Emelianova Luiza Garaeva Alexander Yakimov Marina Sogoyan Michael Filatov Andrey L Konevega Tatiana Shtam Source Type: research

Disruption of the Functional Activity of Neutrophil Granulocytes as a Risk Factor for the Development of Lung Damage in Pregnant Women with COVID-19
Curr Issues Mol Biol. 2024 Jan 25;46(2):1121-1135. doi: 10.3390/cimb46020071.ABSTRACTCurrently, the assessment of immune status in patients with COVID-19 is limited to determining the count of polymorphonuclear leukocytes and the phagocytic function of neutrophils, which is insufficient to understand the regulatory role of innate immunity cells in the development of pneumonia. However, no such studies have been conducted in pregnant women with COVID-19. The aim of this study was to investigate the functional state of neutrophil granulocytes in order to identify predictors of pneumonia severity risk in pregnant women with C...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Irina Anatolyevna Andrievskaya Egor Mikhailovich Ustinov Karen Sargisovich Lyazgian Nataliya Alexandrovna Ishutina Inna Victorovna Dovzhikova Source Type: research

Molecular Characterization and Expression Changes of the < em > bcl2l13 < /em > Gene in Response to Hypoxia in < em > Megalobrama amblycephala < /em >
Curr Issues Mol Biol. 2024 Jan 25;46(2):1136-1149. doi: 10.3390/cimb46020072.ABSTRACTHypoxia is a unique environmental stress, which not only reflects the insufficient oxygen supply of cells and tissues, but also occurs in various physiological and pathological environments. Mitophagy as a selective autophagy can recover and utilize damaged organelles and misfolded proteins to ensure normal cell functions and promote cell survival. Bcl2l13 (B-cell lymphoma-2 like 13) is reported to induce mitophagy as a functional mammalian homolog of Atg32. However, the function of the bcl2l13 gene is still unclear in fish. Here the seque...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Axin Zhang Xuefei Guo Kaikai Bao Danyang Wu Hong Liu Zexia Gao Huanling Wang Source Type: research

Next Generation Sequencing and Electromyography Reveal the Involvement of the < em > P2RX6 < /em > Gene in Myopathy
Curr Issues Mol Biol. 2024 Jan 29;46(2):1150-1163. doi: 10.3390/cimb46020073.ABSTRACTIon channelopathies result from impaired ion channel protein function, due to mutations affecting ion transport across cell membranes. Over 40 diseases, including neuropathy, pain, migraine, epilepsy, and ataxia, are associated with ion channelopathies, impacting electrically excitable tissues and significantly affecting skeletal muscle. Gene mutations affecting transmembrane ionic flow are strongly linked to skeletal muscle disorders, particularly myopathies, disrupting muscle excitability and contraction. Electromyography (EMG) analysis ...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Mirella Vinci Girolamo Aurelio Vitello Donatella Greco Simone Treccarichi Alda Ragalmuto Antonino Musumeci Antonio Fallea Concetta Federico Francesco Cal ì Salvatore Saccone Maurizio Elia Source Type: research

Production of Mature Recombinant Human Activin A in Transgenic Rice Cell Suspension Culture
In this study, the whole gene of the precursor form of recombinant human activin A (rhAA) contains a signal peptide, and a pro-region and a mature region were cloned into an expression vector under the control of the rice α-amylase 3D (RAmy3D) promoter. To obtain the mature (active) form of rhAA, an enterokinase cleavage site was inserted between the pro-region and mature region of rhAA. The rice seed (Oryza sativa L. cv. Dongjin) was transformed with recombinant vectors by the Agrobacterium-mediated method, and the integration of the target gene into the plant genome was confirmed by genomic PCR. The transcript expressio...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Van Giap Do Moon-Sik Yang Source Type: research

Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still's Disease
Curr Issues Mol Biol. 2024 Feb 1;46(2):1177-1191. doi: 10.3390/cimb46020075.ABSTRACTAdult-onset Still's disease (AOSD) is a complex systemic inflammatory disorder, categorized as an 'IL-1 driven' inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Valentina Myachikova Igor Kudryavtsev Artem Rubinstein Arthur Aquino Dmitry Isakov Alexey Golovkin Alexey Maslyanskiy Source Type: research

Exploring Perforated Jejunal GIST: A Rare Case Report and Review of Molecular and Clinical Literature
Curr Issues Mol Biol. 2024 Feb 1;46(2):1192-1207. doi: 10.3390/cimb46020076.ABSTRACTThis case report details a rare instance of a perforated jejunal gastrointestinal stromal tumor (GIST) in a 76-year-old female patient. The patient presented with acute abdominal pain and distension without any changes in bowel habits or episodes of nausea and vomiting. Initial diagnostics, including abdominal plain radiography and ultrasonography, were inconclusive; however, a computed tomography (CT) scan revealed pneumoperitoneum and an irregular fluid collection suggestive of small intestine perforations. Surgical intervention uncovered...
Source: Current Issues in Molecular Biology - February 23, 2024 Category: Molecular Biology Authors: Milos Mirovic Milica Dimitrijevic Stojanovic Marina Jovanovic Vesna Stankovic Danijela Milosev Natasa Zdravkovic Bojan Milosevic Aleksandar Cvetkovic Marko Spasic Berislav Vekic Ivan Jovanovic Bojana S Stojanovic Marko Petrovic Ana Bogut Miodrag Peulic Bo Source Type: research