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Low-Salt Diet Regulates the Metabolic and Signal Transduction Genomic Fabrics, and Remodels the Cardiac Normal and Chronic Pathological Pathways
Curr Issues Mol Biol. 2024 Mar 12;46(3):2355-2385. doi: 10.3390/cimb46030150.ABSTRACTLow-salt diet (LSD) is a constant recommendation to hypertensive patients, but the genomic mechanisms through which it improves cardiac pathophysiology are still not fully understood. Our publicly accessible transcriptomic dataset of the left ventricle myocardium of adult male mice subjected to prolonged LSD or normal diet was analyzed from the perspective of the Genomic Fabric Paradigm. We found that LSD shifted the metabolic priorities by increasing the transcription control for fatty acids biosynthesis while decreasing it for steroid ho...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Dumitru A Iacobas Haile Allen Sanda Iacobas Source Type: research

Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A
Curr Issues Mol Biol. 2024 Mar 13;46(3):2386-2397. doi: 10.3390/cimb46030151.ABSTRACTDimethyloxalylglycine (DMOG) is a representative inhibitor of the prolyl hydroxylase domain (PHD), which mediates the degradation of hypoxia-inducible factor-1-alpha (HIF1A). DMOG exerts its pharmacological effects via the canonical pathway that involves PHD inhibition; however, it remains unclear whether DMOG affects lipogenic gene expression in hepatocytes. We aimed to elucidate the effects of DMOG on sterol regulatory element-binding protein-1c (SREBP1c), a master regulator of fatty acid synthesis in hepatocytes. DMOG treatment inhibite...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Yong Seong Kwon Ye Eun Cho Yeonsoo Kim Minseob Koh Seonghwan Hwang Source Type: research

Editorial for the Special Issue "Molecular Biology in Targeted Radionuclide Therapy Radiopharmaceutical Design"
Curr Issues Mol Biol. 2024 Mar 13;46(3):2398-2401. doi: 10.3390/cimb46030152.ABSTRACTTargeted radionuclide therapy (TRT) is gaining wide and rapid acceptance in clinical practice as it can deliver alpha or beta irradiation to a tumor-associated target which may be present in the tumor cell itself or in the microenvironment [...].PMID:38534768 | PMC:PMC10969543 | DOI:10.3390/cimb46030152 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Carlo Aprile Onelio Geatti Letizia Canziani Lorenzo Lodola Source Type: research

Deciphering Glioblastoma: Fundamental and Novel Insights into the Biology and Therapeutic Strategies of Gliomas
Curr Issues Mol Biol. 2024 Mar 13;46(3):2402-2443. doi: 10.3390/cimb46030153.ABSTRACTGliomas constitute a diverse and complex array of tumors within the central nervous system (CNS), characterized by a wide range of prognostic outcomes and responses to therapeutic interventions. This literature review endeavors to conduct a thorough investigation of gliomas, with a particular emphasis on glioblastoma (GBM), beginning with their classification and epidemiological characteristics, evaluating their relative importance within the CNS tumor spectrum. We examine the immunological context of gliomas, unveiling the intricate immun...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Razvan Onciul Felix-Mircea Brehar Corneliu Toader Razvan-Adrian Covache-Busuioc Luca-Andrei Glavan Bogdan-Gabriel Bratu Horia Petre Costin David-Ioan Dumitrascu Matei Serban Alexandru Vlad Ciurea Source Type: research

The Combination of Oolonghomobisflavan B and Diallyl Disulfide Induces Apoptotic Cell Death via 67-kDa Laminin Receptor/Cyclic Guanosine Monophosphate in Acute Myeloid Leukemia Cells
In conclusion, we show that the combination of OHBFB and DADS synergistically induced apoptotic cell death in AML cells through activation of 67LR/cGMP/PKCδ/ASM signaling pathway. Moreover, in this mechanism, we demonstrate DADS may reduce the enzyme activity of phosphodiesterase, which is a negative regulator of cGMP that potentiates OHBFB-induced AML apoptotic cell death without affecting normal PBMCs.PMID:38534770 | PMC:PMC10969046 | DOI:10.3390/cimb46030154 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Jaehoon Bae Su-Jin Park Source Type: research

Prognostic Value of P63 Expression in Muscle-Invasive Bladder Cancer and Association with Molecular Subtypes-Preliminary Report
Curr Issues Mol Biol. 2024 Mar 14;46(3):2456-2467. doi: 10.3390/cimb46030155.ABSTRACTThere is an ongoing need for biomarkers that could reliably predict the outcome of BC and that could guide the management of this disease. In this setting, we aimed to explore the prognostic value of the transcription factor P63 in patients with muscle-invasive bladder cancer (MIBC) having undergone radical cystectomy. The correlation between P63 expression and clinicopathological features (tumor stage, nodes involvement, patterns of muscularis propria invasion, papillary architecture, anaplasia, concomitant carcinoma in situ, lymphovascul...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Francesca Sanguedolce Ugo Giovanni Falagario Magda Zanelli Andrea Palicelli Maurizio Zizzo Stefano Ascani Simona Tortorella Gian Maria Busetto Angelo Cormio Giuseppe Carrieri Luigi Cormio Source Type: research

5-AZA Upregulates SOCS3 and PTPN6/SHP1, Inhibiting STAT3 and Potentiating the Effects of AG490 against Primary Effusion Lymphoma Cells
This study suggests that 5-AZA, by upregulating the expression level of SOCS3 and PTPN6/SHP1, reduced STAT3 activation and improved the outcome of treatment targeting this transcription factor in PEL cells.PMID:38534772 | PMC:PMC10968859 | DOI:10.3390/cimb46030156 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Michele Di Crosta Andrea Arena Rossella Benedetti Maria Saveria Gilardini Montani Mara Cirone Source Type: research

 N -3-Methylbutyl-benzisoselenazol-3(2H)-one Exerts Antifungal Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis
Curr Issues Mol Biol. 2024 Mar 14;46(3):2480-2496. doi: 10.3390/cimb46030157.ABSTRACTIn the present work, we evaluated the antifungal activities of two novel ebselen analogs, N-allyl-benzisoselenazol-3(2H)-one (N-allyl-bs) and N-3-methylbutylbenzisoselenazol-3(2H)-one (N-3mb-bs). Colorimetric and turbidity assays were performed to determine the minimum inhibitory concentration (MIC) of these compounds in S1 (fluconazole-sensitive) and S2 (fluconazole-resistant) strains of C. albicans. N-3mb-bs was more active than the N-allyl-bs compound. It is noteworthy that the concentration of N-3mb-bs observed to inhibit fungal growth...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Xiuyi Liang Agata J Pacu ła-Miszewska Richa Vartak Milankumar Prajapati Haiyan Zheng Caifeng Zhao Ganming Mao Ketankumar Patel Natalya U Fedosova Jacek Ścianowski Blase Billack Source Type: research

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer
In this study, we present a strain of Bacillus paranthracis for the first time, demonstrating significant potential in the production of phosphatidylcholine-specific phospholipase C (PC-PLC). The investigation thoroughly examines the B. paranthracis PUMB_17 strain, focusing on the activity of PC-PLC and its purification process. Notably, the PUMB_17 strain displays extracellular PC-PLC production with high specific activity during the late exponential growth phase. To unravel the genetic makeup of PUMB_17, we employed nanopore-based whole-genome sequencing and subsequently conducted a detailed genome annotation. The genome...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Vesselin Baev Ivan Iliev Yordan Stefanov Marinela Tsankova Mariana Marhova Elena Apostolova Mariyana Gozmanova Galina Yahubyan Sonya Kostadinova Source Type: research

Complete Mitochondrial Genomes of Nedyopus patrioticus : New Insights into the Color Polymorphism of Millipedes
Curr Issues Mol Biol. 2024 Mar 15;46(3):2514-2527. doi: 10.3390/cimb46030159.ABSTRACTThere has been debate about whether individuals with different color phenotypes should have different taxonomic status. In order to determine whether the different color phenotypes of Nedyopus patrioticus require separate taxonomic status or are simply synonyms, here, the complete mitochondrial genomes (mitogenomes) of two different colored N. patrioticus, i.e., red N. patrioticus and white N. patrioticus, are presented. The two mitogenomes were 15,781 bp and 15,798 bp in length, respectively. Each mitogenome contained 13 PCGs, 19 tRNAs, 2...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Gaoji Zhang Tangjun Xu Yukun Chen Wei Xu Yinuo Wang Yuanyuan Li Fuyuan Zhu Hongyi Liu Honghua Ruan Source Type: research

The Influence of Neurotrophins on the Brain-Lung Axis: Conception, Pregnancy, and Neonatal Period
Curr Issues Mol Biol. 2024 Mar 15;46(3):2528-2543. doi: 10.3390/cimb46030160.ABSTRACTNeurotrophins (NTs) are four small proteins produced by both neuronal and non-neuronal cells; they include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NTs can exert their action through both genomic and non-genomic mechanisms by interacting with specific receptors. Initial studies on NTs have identified them only as functional molecules of the nervous system. However, recent research have shown that some tissues and organs (such as the lungs, skin, and skeletal and ...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Federica D'Amico Cecilia Lugar à Giovanni Luppino Carlo Giuffrida Ylenia Giorgianni Eleonora Maria Patan è Sara Manti Antonella Gambadauro Mariarosaria La Rocca Tiziana Abbate Source Type: research

The NLRP3 Inflammasome Gene Is Overexpressed in Hidradenitis Suppurativa Lesions: A Preliminary Study on the Role of Pyroptosis in Disease Pathogenesis
This study aimed to investigate the role of the P2X7 receptor (P2X7R) and NLRP3 inflammasome in HS pathogenesis. RNA sequencing and real-time PCR were performed to assess the gene expression levels of P2X7R and NLRP3 in the skin biopsies of HS patients and healthy controls (HC). The results of our study revealed a significantly increased expression of the NLRP3 gene in both the lesional and perilesional skin of HS patients compared to healthy controls. Moreover, the mRNA levels of NLRP3 were significantly higher in lesional skin compared to non-lesional skin in HS patients, indicating the spread of inflammation to adjacent...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Piotr K Krajewski Weronika Szuka ła Jacek C Szepietowski Source Type: research

Genetic and Environmental Factors in Autoimmune Thyroid Disease: Exploring Associations with Selenium Levels and Novel Loci in a Latvian Cohort
Curr Issues Mol Biol. 2024 Mar 17;46(3):2553-2565. doi: 10.3390/cimb46030162.ABSTRACTThe interplay of genetic, immune and environmental factors strongly contributes to the development of autoimmune thyroid disease (AITD), which can be classified as Graves' disease (GD) or Hashimoto thyroiditis (HT). One of the most studied exogenous factors in the pathogenesis of AITD is selenium, which, in the form of selenoproteins, strengthens the antioxidative defence system of thyroid cells against superoxide production. Furthermore, it modulates inflammatory cytokine release and autoantibody production. The aim of this study was to a...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Sabine Upmale-Engela Ieva Vaivode Raitis Peculis Helena Litvina Tatjana Zake Andrejs Skesters Deniss Gogins Vita Rovite Ilze Konrade Source Type: research

Autosomal Recessive Rod-Cone Dystrophy with Mild Extra-Ocular Manifestations Due to a Splice-Affecting Variant in BBS9 
Curr Issues Mol Biol. 2024 Mar 18;46(3):2566-2575. doi: 10.3390/cimb46030163.ABSTRACTBardet-Biedl syndrome (BBS), one of the most common forms of syndromic inherited retinal diseases (IRDs), is characterized by the combination of retinal degeneration with additional extra-ocular manifestations, including obesity, intellectual disability, kidney disease, polydactyly and other skeletal abnormalities. We observed an Israeli patient with autosomal recessive apparently non-syndromic rod-cone dystrophy (RCD). Extra-ocular findings were limited to epilepsy and dental problems. Genetic analysis with a single molecule molecular inv...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Iris Deitch Sofia Itskov Daan Panneman Aasem Abu Shtaya Tal Saban Yael Goldberg Miriam Ehrenberg Frans P M Cremers Susanne Roosing Tamar Ben-Yosef Source Type: research

Effects of rpl1001 Gene Deletion on Cell Division of Fission Yeast and Its Molecular Mechanism
This study focuses on the growth, spore production, cell morphology, the dynamics of microtubules, chromosomes, actin, myosin, and mitochondria of fission yeast (Schizosaccharomyces pombe) to investigate the impact of rpl1001 deletion on cell mitosis. RNA-Seq and bioinformatics analyses were also used to reveal key genes, such as hsp16, mfm1 and isp3, and proteasome pathways. The results showed that rpl1001 deletion resulted in slow cell growth, abnormal spore production, altered cell morphology, and abnormal microtubule number and length during interphase. The cell dynamics of the rpl1001Δ strain showed that the formatio...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Wen Yu Rongmei Yuan Mengnan Liu Ke Liu Xiang Ding Yiling Hou Source Type: research