PRI-724 and IWP-O1 Wnt Signaling Pathway Inhibitors Modulate the Expression of Glycolytic Enzymes in Tongue Cancer Cell Lines
Curr Issues Mol Biol. 2023 Nov 29;45(12):9579-9592. doi: 10.3390/cimb45120599.ABSTRACTThe dysregulation of energetic metabolism is one of the hallmarks of cancer cells. Indeed, the growth of head and neck squamous cell carcinoma (HNSCC) cells depends heavily on glycolytic activity, which can be considered a potential therapeutic target. Wnt signaling is one of the pathways that undergoes upregulation in HNSCC. Our previous studies have shown that Wnt signaling inhibitors-PRI-724 and IWP-O1-attenuate tongue SCC survival and reduce glucose uptake and lactate release. The aim of this research was to further evaluate the possi...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Robert Kleszcz Jaros ław Paluszczak Marta Belka Violetta Krajka-Ku źniak Source Type: research

The Role of Adopted Orphan Nuclear Receptors in the Regulation of an Organic Anion Transporting Polypeptide 1B1 (OATP1B1) under the Action of Sex Hormones
Curr Issues Mol Biol. 2023 Nov 29;45(12):9593-9605. doi: 10.3390/cimb45120600.ABSTRACTOrganic anion transporting polypeptide 1B1 (OATP1B1) is an influx transporter protein of the SLC superfamily, expressed mainly in the liver and some tumor cells. The mechanisms of its regulation are being actively studied. In the present study, the effect of sex hormones (estradiol, progesterone and testosterone) on OATP1B1 expression in HepG2 cells was examined. The role of adopted orphan receptors, farnasoid X receptor (FXR), constitutive androstane receptor (CAR), pregnane X receptor (PXR) and liver X receptor subtype alpha (LXRa), was...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Aleksey V Shchulkin Yulia V Abalenikhina Aleksandr A Slepnev Egor D Rokunov Elena N Yakusheva Source Type: research

Integrative Analysis of the Role of < em > TP53 < /em > in Human Pan-Cancer
This study provides a theoretical basis for clinical TP53 gene therapy.PMID:38132447 | PMC:PMC10742156 | DOI:10.3390/cimb45120601 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Tingting Liu Jin Du Xiangshu Cheng Jianshe Wei Source Type: research

Identification of Multiple Genetic Loci Related to Low-Temperature Tolerance during Germination in Maize ( < em > Zea maize < /em > L.) through a Genome-Wide Association Study
In this study, 296 excellent inbred lines from domestic and foreign origins (America and Europe) were used as the study materials, and a low-coverage resequencing method was employed for genome sequencing. Five phenotypic traits related to low-temperature tolerance were used to assess the genetic diversity of maize through a genome-wide association study (GWAS). A total of 14 SNPs significantly associated with low-temperature tolerance were detected (-log10(P) > 4), and an SNP consistently linked to low-temperature tolerance in the field and indoors during germination was utilized as a marker. This SNP, 14,070, was loca...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Tao Yu Jianguo Zhang Jingsheng Cao Shujun Li Quan Cai Xin Li Sinan Li Yunlong Li Changan He Xuena Ma Source Type: research

Analysis of DNA Methylation Differences during the JIII Formation of < em > Bursaphelenchus xylophilus < /em >
Curr Issues Mol Biol. 2023 Nov 30;45(12):9656-9673. doi: 10.3390/cimb45120603.ABSTRACTDNA methylation is a pivotal process that regulates gene expression and facilitates rapid adaptation to challenging environments. The pinewood nematode (PWN; Bursaphelenchus xylophilus), the causative agent of pine wilt disease, survives at low temperatures through third-stage dispersal juvenile, making it a major pathogen for pines in Asia. To comprehend the impact of DNA methylation on the formation and environmental adaptation of third-stage dispersal juvenile, we conducted whole-genome bisulfite sequencing and transcriptional sequenci...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Peng Wang Yongxia Li Zhenkai Liu Wei Zhang Dongzhen Li Xuan Wang Xiaojian Wen Yuqian Feng Xingyao Zhang Source Type: research

< em > Ginkgo biloba < /em > : Antioxidant Activity and In Silico Central Nervous System Potential
Curr Issues Mol Biol. 2023 Dec 1;45(12):9674-9691. doi: 10.3390/cimb45120604.ABSTRACTGinkgo biloba (GB) extracts have been used in clinical studies as an alternative therapy for Alzheimer's disease (AD), but the exact bioaction mechanism has not yet been elucidated. In this work, an in silico study on GB metabolites was carried out using SwissTargetPrediction to determine the proteins associated with AD. The resulting proteins, AChE, MAO-A, MAO-B, β-secretase and γ-secretase, were studied by molecular docking, resulting in the finding that kaempferol, quercetin, and luteolin have multitarget potential against AD. These c...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Eduardo Su árez-González Jes ús Sandoval-Ramírez Jorge Flores-Hern ández Alan Carrasco-Carballo Source Type: research

Overexpression of < em > KvCHX < /em > Enhances Salt Tolerance in < em > Arabidopsis thaliana < /em > Seedlings
Curr Issues Mol Biol. 2023 Dec 1;45(12):9692-9708. doi: 10.3390/cimb45120605.ABSTRACTThe CHX (cation/H+ exchanger) family plays an important role in the transmembrane transport of cation/H+ in plants. The aim of this study was to identify and functionally analyze the KvCHX gene in the halophyte Kosteletzkya virginica to investigate its role in regulating the K+/Na+ ratio under salinity tolerance. Based on a partial gene sequence of EST from K. virginica, the full-length DNA sequence of the KvCHX gene was obtained using genome walking technology. Structural analysis and phylogenetic relationship analysis showed that the KvC...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Yuqi Guo Chengrong Zhu Zengyuan Tian Source Type: research

A Comparative Kidney Transcriptome Analysis of Bicarbonate-Loaded < em > insrr < /em > -Null Mice
Curr Issues Mol Biol. 2023 Dec 4;45(12):9709-9722. doi: 10.3390/cimb45120606.ABSTRACTThe maintenance of plasma pH is critical for life in all organisms. The kidney plays a critical role in acid-base regulation in vertebrates by controlling the plasma concentration of bicarbonate. The receptor tyrosine kinase IRR (insulin receptor-related receptor) is expressed in renal β-intercalated cells and is involved in alkali sensing due to its ability to autophosphorylate under alkalization of extracellular medium (pH > 7.9). In mice with a knockout of the insrr gene, which encodes for IRR, urinary bicarbonate secretion in respo...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: E A Gantsova O V Serova D Eladari D M Bobrovskiy A G Petrenko A V Elchaninov I E Deyev Source Type: research

Integrated Analysis of Transcriptome and Metabolome Profiles in the Longissimus Dorsi Muscle of Buffalo and Cattle
This study contributes to the breed genetic improvement and enhancement of buffalo meat quality.PMID:38132453 | PMC:PMC10741837 | DOI:10.3390/cimb45120607 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Guansheng Wu Xinjun Qiu Zizhuo Jiao Weijie Yang Haoju Pan Hong Li Zhengyu Bian Qiang Geng Hui Wu Junming Jiang Yuanyuan Chen Yiwen Cheng Qiaoling Chen Si Chen Churiga Man Li Du Lianbin Li Fengyang Wang Source Type: research

A Bioinformatics Toolkit for Next-Generation Sequencing in Clinical Oncology
Curr Issues Mol Biol. 2023 Dec 4;45(12):9737-9752. doi: 10.3390/cimb45120608.ABSTRACTNext-generation sequencing (NGS) has taken on major importance in clinical oncology practice. With the advent of targeted therapies capable of effectively targeting specific genomic alterations in cancer patients, the development of bioinformatics processes has become crucial. Thus, bioinformatics pipelines play an essential role not only in the detection and in identification of molecular alterations obtained from NGS data but also in the analysis and interpretation of variants, making it possible to transform raw sequencing data into mea...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Simon Cabello-Aguilar Julie A Vendrell J érôme Solassol Source Type: research

Metabolic Interplay in the Tumor Microenvironment: Implications for Immune Function and Anticancer Response
Curr Issues Mol Biol. 2023 Dec 5;45(12):9753-9767. doi: 10.3390/cimb45120609.ABSTRACTMalignant tumors exhibit rapid growth and high metabolic rates, similar to embryonic stem cells, and depend on aerobic glycolysis, known as the "Warburg effect". This understanding has enabled the use of radiolabeled glucose analogs in tumor staging and therapeutic response assessment via PET scans. Traditional treatments like chemotherapy and radiotherapy target rapidly dividing cells, causing significant toxicity. Despite immunotherapy's impact on solid tumor treatment, gaps remain, leading to research on cancer cell evasion of immune re...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Reem Youssef Rohan Maniar Jaffar Khan Hector Mesa Source Type: research

DNA Methylation of the IL-17A Gene Promoter Is Associated with Subclinical Atherosclerosis and Coronary Artery Disease: The Genetics of Atherosclerotic Disease Mexican Study
Curr Issues Mol Biol. 2023 Dec 5;45(12):9768-9777. doi: 10.3390/cimb45120610.ABSTRACTThe interleukin-17 (IL-17) has a crucial role during inflammation and has been associated with cardiovascular diseases, but its role in epigenetics is still poorly understood. Therefore, the aim of this study was to evaluate the DNA methylation status of the IL-17A gene promoter to establish whether it may represent a risk factor for subclinical atherosclerosis (SA) or clinical coronary artery disease (CAD). We included 38 patients with premature CAD (pCAD), 48 individuals with SA, and 43 healthy controls. Methylation in the CpG region of ...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Nonanzit P érez-Hernández Rosalinda Posadas-S ánchez Gilberto Vargas-Alarc ón Óscar Pérez-Méndez Mar ía Luna-Luna Jos é Manuel Rodríguez-Pérez Source Type: research

Impact of Hypoxia-Induced miR-210 on Pancreatic Cancer
Curr Issues Mol Biol. 2023 Dec 5;45(12):9778-9792. doi: 10.3390/cimb45120611.ABSTRACTPancreatic cancer (PC) poses significant clinical challenges, with late-stage diagnosis and limited therapeutic options contributing to its dismal prognosis. A hallmark feature of PC is the presence of a profoundly hypoxic tumour microenvironment, resulting from various factors such as fibrotic stroma, rapid tumour cell proliferation, and poor vascularization. Hypoxia plays a crucial role in promoting aggressive cancer behaviour, therapeutic resistance, and immunosuppression. Previous studies have explored the molecular mechanisms behind h...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Mutian Lian Maria Mortoglou Pinar Uysal-Onganer Source Type: research

Cationic Porphyrins as Antimicrobial and Antiviral Agents in Photodynamic Therapy
Curr Issues Mol Biol. 2023 Dec 6;45(12):9793-9822. doi: 10.3390/cimb45120612.ABSTRACTAntimicrobial photodynamic therapy (APDT) has received a great deal of attention due to its unique ability to kill all currently known classes of microorganisms. To date, infectious diseases caused by bacteria and viruses are one of the main sources of high mortality, mass epidemics and global pandemics among humans. Every year, the emergence of three to four previously unknown species of viruses dangerous to humans is recorded, totaling more than 2/3 of all newly discovered human pathogens. The emergence of bacteria with multidrug resista...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Inga O Savelyeva Kseniya A Zhdanova Margarita A Gradova Oleg V Gradov Natal'ya A Bragina Source Type: research

Amelioration of Motor Performance and Nigrostriatal Dopamine Cell Volume Using a Novel Far-Infrared Ceramic Blanket in an A53T Alpha-Synuclein Transgenic Parkinson's Disease Mouse Model
Curr Issues Mol Biol. 2023 Dec 6;45(12):9823-9837. doi: 10.3390/cimb45120613.ABSTRACTWe had attended a Parkinson's Disease (PD) patient for a non-healing wound who reported a marked decrease in his hand tremor and freezing of gait when his wound was exposed to a ceramic far-field infrared (cFIR) blanket. PD is the most frequent motor disorder and the second most frequent neurodegenerative disease after Alzheimer's Disease (AD). The tremor, rigidity, and slowness of movement associated with Parkinson's disease (PD) affect up to 10 million people throughout the world, and the major contributing factor to the pathogenesis of ...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Frederick Robert Carrick Luis Sebastian Alexis Valerio Hernandez Kiminobu Sugaya Source Type: research