Inflammation Factors and Genistein Supplementation in Cancer-Preliminary Research
Curr Issues Mol Biol. 2024 Mar 7;46(3):2166-2180. doi: 10.3390/cimb46030140.ABSTRACTThe purpose of this study was to evaluate the effect of genistein in nano, micro, and macro forms on the intensity of the DMBA-induced tumor process in rats and to understand the mechanisms of this action. The effect of genistein supplementation on the content of selected eicosanoids (HETEs, HODE, and HEPE) in the serum of rats was evaluated. The levels and expression of genes encoding various pro-inflammatory cytokines (IL-1, IL-6) and MMP-9 in the blood of rats were also investigated. The biological material for the study was blood obtain...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Karolina Bany ś Ma łgorzata Jelińska Ma łgorzata Wrzosek Dorota Skrajnowska Robert Wrzesie ń Wojciech Bielecki Barbara Bobrowska-Korczak Source Type: research

To Repair a Broken Heart: Stem Cells in Ischemic Heart Disease
Curr Issues Mol Biol. 2024 Mar 8;46(3):2181-2208. doi: 10.3390/cimb46030141.ABSTRACTDespite improvements in contemporary medical and surgical therapies, cardiovascular disease (CVD) remains a significant cause of worldwide morbidity and mortality; more specifically, ischemic heart disease (IHD) may affect individuals as young as 20 years old. Typically managed with guideline-directed medical therapy, interventional or surgical methods, the incurred cardiomyocyte loss is not always completely reversible; however, recent research into various stem cell (SC) populations has highlighted their potential for the treatment and pe...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Theodora M Stougiannou Konstantinos C Christodoulou Ioannis Dimarakis Dimitrios Mikroulis Dimos Karangelis Source Type: research

Autophagy-Dependent Secretion: Crosstalk between Autophagy and Exosome Biogenesis
Curr Issues Mol Biol. 2024 Mar 8;46(3):2209-2235. doi: 10.3390/cimb46030142.ABSTRACTThe cellular secretome is pivotal in mediating intercellular communication and coordinating responses to stressors. Exosomes, initially recognized for their role in waste disposal, have now emerged as key intercellular messengers with significant therapeutic and diagnostic potential. Similarly, autophagy has transcended its traditional role as a waste removal mechanism, emerging as a regulator of intracellular communication pathways and a contributor to a unique autophagy-dependent secretome. Secretory authophagy, initiated by various stres...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Ekaterina Zubkova Alexander Kalinin Anastasya Bolotskaya Irina Beloglazova Mikhail Menshikov Source Type: research

The Beneficial Role of < em > Lactobacillus paracasei < /em > subsp. < em > paracasei < /em > NTU 101 in the Prevention of Atopic Dermatitis
In this study, Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) was orally administered for a duration of 4 weeks, followed by the induction of AD using ovalbumin (OVA) in a mouse model. The skin condition of the stimulated site was observed during the induction period. Subsequently, the serum immunoglobulin E (IgE) content, splenocyte T cell typing, and skin histological interpretation were examined to evaluate the efficacy of NTU 101 in alleviating AD symptoms in allergen-exposed animals. The findings indicated that administering NTU 101 beforehand effectively alleviated skin symptoms in animals with AD. It red...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Chin-Feng Liu Tsung-Wei Shih Chun-Lin Lee Tzu-Ming Pan Source Type: research

A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145
Curr Issues Mol Biol. 2024 Mar 10;46(3):2251-2262. doi: 10.3390/cimb46030144.ABSTRACTProstate cancer accounts for 14% of male cancer-related fatalities in the UK. Given the challenges associated with hormone-based therapies in the context of androgen-independent prostate cancer, there is an imperative need for research into anticancer drugs. N0821, a peptide belonging to the Trp-Arg dense region and derived from the homologous region of various bee species, shows substantial potential for an anticancer effect. Both MTT assays and 3D spheroid assays were conducted to substantiate its antiproliferation potential and strongly...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Ye-Eun Kim Ki-Young Kim Source Type: research

Genetic Signatures for Distinguishing Chemo-Sensitive from Chemo-Resistant Responders in Prostate Cancer Patients
This study delves into the expression of drug resistance-associated genes, ABCB1 and CYP1B1, in cancer cells. Employing bioinformatics, we explored the molecular pathways and cascades linked to drug resistance following upregulation of these genes. Samples were obtained from archived prostate cancer patient specimens through pre-treatment biopsies of two categories: good vs. poor responders, with cDNAs synthesized from isolated RNAs subjected to qPCR analysis. The results revealed increased ABCB1 and CYP1B1 expression in tumor samples of the poor responders. Gene enrichment and network analysis associated ABCB1 with ABC tr...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Lemohang Gumenku Mamello Sekhoacha Beynon Abrahams Samson Mashele Aubrey Shoko Ochuko L Erukainure Source Type: research

Genome-Wide Analysis of < em > VILLIN < /em > Gene Family Associated with Stress Responses in Cotton ( < em > Gossypium < /em > spp.)
Curr Issues Mol Biol. 2024 Mar 11;46(3):2278-2300. doi: 10.3390/cimb46030146.ABSTRACTThe VILLIN (VLN) protein plays a crucial role in regulating the actin cytoskeleton, which is involved in numerous developmental processes, and is crucial for plant responses to both biotic and abiotic factors. Although various plants have been studied to understand the VLN gene family and its potential functions, there has been limited exploration of VLN genes in Gossypium and fiber crops. In the present study, we characterized 94 VLNs from Gossypium species and 101 VLNs from related higher plants such as Oryza sativa and Zea mays and some...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Akash Deep Dhananjay K Pandey Source Type: research

Unveiling the Ovarian Cell Characteristics and Molecular Mechanism of Prolificacy in Goats via Single-Nucleus Transcriptomics Data Analysis
This study provides a comprehensive understanding of the cell-type-specific mechanisms and regulatory networks in the goat ovary, providing insights into the molecular mechanisms underlying goat prolificacy. These findings establish a vital foundation for furthering understanding of the molecular mechanisms governing folliculogenesis and for improving the litter size in goats via molecular design breeding.PMID:38534763 | PMC:PMC10969506 | DOI:10.3390/cimb46030147 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Sanbao Zhang Yirong Wei Xiaotong Gao Ying Song Yanna Huang Qinyang Jiang Source Type: research

Bioconversion, Pharmacokinetics, and Therapeutic Mechanisms of Ginsenoside Compound K and Its Analogues for Treating Metabolic Diseases
Curr Issues Mol Biol. 2024 Mar 11;46(3):2320-2342. doi: 10.3390/cimb46030148.ABSTRACTRare ginsenoside compound K (CK) is an intestinal microbial metabolite with a low natural abundance that is primarily produced by physicochemical processing, side chain modification, or metabolic transformation in the gut. Moreover, CK exhibits potent biological activity compared to primary ginsenosides, which has raised concerns in the field of ginseng research and development, as well as ginsenoside-related dietary supplements and natural products. Ginsenosides Rb1, Rb2, and Rc are generally used as a substrate to generate CK via several...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Md Niaj Morshed Reshmi Akter Md Rezaul Karim Safia Iqbal Se Chan Kang Deok Chun Yang Source Type: research

Antioxidant Glutathione Analogues UPF1 and UPF17 Modulate the Expression of Enzymes Involved in the Pathophysiology of Chronic Obstructive Pulmonary Disease
Curr Issues Mol Biol. 2024 Mar 12;46(3):2343-2354. doi: 10.3390/cimb46030149.ABSTRACTIncreased oxidative stress (OS) and systemic inflammation are key players in the pathophysiology of chronic obstructive pulmonary disease (COPD). We aimed to clarify the effects of synthetic glutathione (GSH) analogue peptides UPF1 and UPF17 on the mRNA levels of enzymes involved in systemic inflammation and GSH metabolism in peripheral blood mononuclear cells (PBMCs) from patients with acute exacerbation of COPD (AE-COPD) and stable COPD along with non-obstructive smokers and non-smokers. UPF1 and UPF17 increased the expression of enzymes...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Ingrid Oit-Wiscombe Ursel Soomets Alan Altraja Source Type: research

Low-Salt Diet Regulates the Metabolic and Signal Transduction Genomic Fabrics, and Remodels the Cardiac Normal and Chronic Pathological Pathways
Curr Issues Mol Biol. 2024 Mar 12;46(3):2355-2385. doi: 10.3390/cimb46030150.ABSTRACTLow-salt diet (LSD) is a constant recommendation to hypertensive patients, but the genomic mechanisms through which it improves cardiac pathophysiology are still not fully understood. Our publicly accessible transcriptomic dataset of the left ventricle myocardium of adult male mice subjected to prolonged LSD or normal diet was analyzed from the perspective of the Genomic Fabric Paradigm. We found that LSD shifted the metabolic priorities by increasing the transcription control for fatty acids biosynthesis while decreasing it for steroid ho...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Dumitru A Iacobas Haile Allen Sanda Iacobas Source Type: research

Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A
Curr Issues Mol Biol. 2024 Mar 13;46(3):2386-2397. doi: 10.3390/cimb46030151.ABSTRACTDimethyloxalylglycine (DMOG) is a representative inhibitor of the prolyl hydroxylase domain (PHD), which mediates the degradation of hypoxia-inducible factor-1-alpha (HIF1A). DMOG exerts its pharmacological effects via the canonical pathway that involves PHD inhibition; however, it remains unclear whether DMOG affects lipogenic gene expression in hepatocytes. We aimed to elucidate the effects of DMOG on sterol regulatory element-binding protein-1c (SREBP1c), a master regulator of fatty acid synthesis in hepatocytes. DMOG treatment inhibite...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Yong Seong Kwon Ye Eun Cho Yeonsoo Kim Minseob Koh Seonghwan Hwang Source Type: research

Editorial for the Special Issue "Molecular Biology in Targeted Radionuclide Therapy Radiopharmaceutical Design"
Curr Issues Mol Biol. 2024 Mar 13;46(3):2398-2401. doi: 10.3390/cimb46030152.ABSTRACTTargeted radionuclide therapy (TRT) is gaining wide and rapid acceptance in clinical practice as it can deliver alpha or beta irradiation to a tumor-associated target which may be present in the tumor cell itself or in the microenvironment [...].PMID:38534768 | PMC:PMC10969543 | DOI:10.3390/cimb46030152 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Carlo Aprile Onelio Geatti Letizia Canziani Lorenzo Lodola Source Type: research

Deciphering Glioblastoma: Fundamental and Novel Insights into the Biology and Therapeutic Strategies of Gliomas
Curr Issues Mol Biol. 2024 Mar 13;46(3):2402-2443. doi: 10.3390/cimb46030153.ABSTRACTGliomas constitute a diverse and complex array of tumors within the central nervous system (CNS), characterized by a wide range of prognostic outcomes and responses to therapeutic interventions. This literature review endeavors to conduct a thorough investigation of gliomas, with a particular emphasis on glioblastoma (GBM), beginning with their classification and epidemiological characteristics, evaluating their relative importance within the CNS tumor spectrum. We examine the immunological context of gliomas, unveiling the intricate immun...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Razvan Onciul Felix-Mircea Brehar Corneliu Toader Razvan-Adrian Covache-Busuioc Luca-Andrei Glavan Bogdan-Gabriel Bratu Horia Petre Costin David-Ioan Dumitrascu Matei Serban Alexandru Vlad Ciurea Source Type: research

The Combination of Oolonghomobisflavan B and Diallyl Disulfide Induces Apoptotic Cell Death via 67-kDa Laminin Receptor/Cyclic Guanosine Monophosphate in Acute Myeloid Leukemia Cells
In conclusion, we show that the combination of OHBFB and DADS synergistically induced apoptotic cell death in AML cells through activation of 67LR/cGMP/PKCδ/ASM signaling pathway. Moreover, in this mechanism, we demonstrate DADS may reduce the enzyme activity of phosphodiesterase, which is a negative regulator of cGMP that potentiates OHBFB-induced AML apoptotic cell death without affecting normal PBMCs.PMID:38534770 | PMC:PMC10969046 | DOI:10.3390/cimb46030154 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Jaehoon Bae Su-Jin Park Source Type: research