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Genetic Signatures for Distinguishing Chemo-Sensitive from Chemo-Resistant Responders in Prostate Cancer Patients
This study delves into the expression of drug resistance-associated genes, ABCB1 and CYP1B1, in cancer cells. Employing bioinformatics, we explored the molecular pathways and cascades linked to drug resistance following upregulation of these genes. Samples were obtained from archived prostate cancer patient specimens through pre-treatment biopsies of two categories: good vs. poor responders, with cDNAs synthesized from isolated RNAs subjected to qPCR analysis. The results revealed increased ABCB1 and CYP1B1 expression in tumor samples of the poor responders. Gene enrichment and network analysis associated ABCB1 with ABC tr...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Lemohang Gumenku Mamello Sekhoacha Beynon Abrahams Samson Mashele Aubrey Shoko Ochuko L Erukainure Source Type: research

Genome-Wide Analysis of VILLIN Gene Family Associated with Stress Responses in Cotton ( Gossypium spp.)
Curr Issues Mol Biol. 2024 Mar 11;46(3):2278-2300. doi: 10.3390/cimb46030146.ABSTRACTThe VILLIN (VLN) protein plays a crucial role in regulating the actin cytoskeleton, which is involved in numerous developmental processes, and is crucial for plant responses to both biotic and abiotic factors. Although various plants have been studied to understand the VLN gene family and its potential functions, there has been limited exploration of VLN genes in Gossypium and fiber crops. In the present study, we characterized 94 VLNs from Gossypium species and 101 VLNs from related higher plants such as Oryza sativa and Zea mays and some...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Akash Deep Dhananjay K Pandey Source Type: research

Unveiling the Ovarian Cell Characteristics and Molecular Mechanism of Prolificacy in Goats via Single-Nucleus Transcriptomics Data Analysis
This study provides a comprehensive understanding of the cell-type-specific mechanisms and regulatory networks in the goat ovary, providing insights into the molecular mechanisms underlying goat prolificacy. These findings establish a vital foundation for furthering understanding of the molecular mechanisms governing folliculogenesis and for improving the litter size in goats via molecular design breeding.PMID:38534763 | PMC:PMC10969506 | DOI:10.3390/cimb46030147 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Sanbao Zhang Yirong Wei Xiaotong Gao Ying Song Yanna Huang Qinyang Jiang Source Type: research

Bioconversion, Pharmacokinetics, and Therapeutic Mechanisms of Ginsenoside Compound K and Its Analogues for Treating Metabolic Diseases
Curr Issues Mol Biol. 2024 Mar 11;46(3):2320-2342. doi: 10.3390/cimb46030148.ABSTRACTRare ginsenoside compound K (CK) is an intestinal microbial metabolite with a low natural abundance that is primarily produced by physicochemical processing, side chain modification, or metabolic transformation in the gut. Moreover, CK exhibits potent biological activity compared to primary ginsenosides, which has raised concerns in the field of ginseng research and development, as well as ginsenoside-related dietary supplements and natural products. Ginsenosides Rb1, Rb2, and Rc are generally used as a substrate to generate CK via several...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Md Niaj Morshed Reshmi Akter Md Rezaul Karim Safia Iqbal Se Chan Kang Deok Chun Yang Source Type: research

Antioxidant Glutathione Analogues UPF1 and UPF17 Modulate the Expression of Enzymes Involved in the Pathophysiology of Chronic Obstructive Pulmonary Disease
Curr Issues Mol Biol. 2024 Mar 12;46(3):2343-2354. doi: 10.3390/cimb46030149.ABSTRACTIncreased oxidative stress (OS) and systemic inflammation are key players in the pathophysiology of chronic obstructive pulmonary disease (COPD). We aimed to clarify the effects of synthetic glutathione (GSH) analogue peptides UPF1 and UPF17 on the mRNA levels of enzymes involved in systemic inflammation and GSH metabolism in peripheral blood mononuclear cells (PBMCs) from patients with acute exacerbation of COPD (AE-COPD) and stable COPD along with non-obstructive smokers and non-smokers. UPF1 and UPF17 increased the expression of enzymes...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Ingrid Oit-Wiscombe Ursel Soomets Alan Altraja Source Type: research

Low-Salt Diet Regulates the Metabolic and Signal Transduction Genomic Fabrics, and Remodels the Cardiac Normal and Chronic Pathological Pathways
Curr Issues Mol Biol. 2024 Mar 12;46(3):2355-2385. doi: 10.3390/cimb46030150.ABSTRACTLow-salt diet (LSD) is a constant recommendation to hypertensive patients, but the genomic mechanisms through which it improves cardiac pathophysiology are still not fully understood. Our publicly accessible transcriptomic dataset of the left ventricle myocardium of adult male mice subjected to prolonged LSD or normal diet was analyzed from the perspective of the Genomic Fabric Paradigm. We found that LSD shifted the metabolic priorities by increasing the transcription control for fatty acids biosynthesis while decreasing it for steroid ho...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Dumitru A Iacobas Haile Allen Sanda Iacobas Source Type: research

Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A
Curr Issues Mol Biol. 2024 Mar 13;46(3):2386-2397. doi: 10.3390/cimb46030151.ABSTRACTDimethyloxalylglycine (DMOG) is a representative inhibitor of the prolyl hydroxylase domain (PHD), which mediates the degradation of hypoxia-inducible factor-1-alpha (HIF1A). DMOG exerts its pharmacological effects via the canonical pathway that involves PHD inhibition; however, it remains unclear whether DMOG affects lipogenic gene expression in hepatocytes. We aimed to elucidate the effects of DMOG on sterol regulatory element-binding protein-1c (SREBP1c), a master regulator of fatty acid synthesis in hepatocytes. DMOG treatment inhibite...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Yong Seong Kwon Ye Eun Cho Yeonsoo Kim Minseob Koh Seonghwan Hwang Source Type: research

Editorial for the Special Issue "Molecular Biology in Targeted Radionuclide Therapy Radiopharmaceutical Design"
Curr Issues Mol Biol. 2024 Mar 13;46(3):2398-2401. doi: 10.3390/cimb46030152.ABSTRACTTargeted radionuclide therapy (TRT) is gaining wide and rapid acceptance in clinical practice as it can deliver alpha or beta irradiation to a tumor-associated target which may be present in the tumor cell itself or in the microenvironment [...].PMID:38534768 | PMC:PMC10969543 | DOI:10.3390/cimb46030152 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Carlo Aprile Onelio Geatti Letizia Canziani Lorenzo Lodola Source Type: research

Deciphering Glioblastoma: Fundamental and Novel Insights into the Biology and Therapeutic Strategies of Gliomas
Curr Issues Mol Biol. 2024 Mar 13;46(3):2402-2443. doi: 10.3390/cimb46030153.ABSTRACTGliomas constitute a diverse and complex array of tumors within the central nervous system (CNS), characterized by a wide range of prognostic outcomes and responses to therapeutic interventions. This literature review endeavors to conduct a thorough investigation of gliomas, with a particular emphasis on glioblastoma (GBM), beginning with their classification and epidemiological characteristics, evaluating their relative importance within the CNS tumor spectrum. We examine the immunological context of gliomas, unveiling the intricate immun...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Razvan Onciul Felix-Mircea Brehar Corneliu Toader Razvan-Adrian Covache-Busuioc Luca-Andrei Glavan Bogdan-Gabriel Bratu Horia Petre Costin David-Ioan Dumitrascu Matei Serban Alexandru Vlad Ciurea Source Type: research

The Combination of Oolonghomobisflavan B and Diallyl Disulfide Induces Apoptotic Cell Death via 67-kDa Laminin Receptor/Cyclic Guanosine Monophosphate in Acute Myeloid Leukemia Cells
In conclusion, we show that the combination of OHBFB and DADS synergistically induced apoptotic cell death in AML cells through activation of 67LR/cGMP/PKCδ/ASM signaling pathway. Moreover, in this mechanism, we demonstrate DADS may reduce the enzyme activity of phosphodiesterase, which is a negative regulator of cGMP that potentiates OHBFB-induced AML apoptotic cell death without affecting normal PBMCs.PMID:38534770 | PMC:PMC10969046 | DOI:10.3390/cimb46030154 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Jaehoon Bae Su-Jin Park Source Type: research

Prognostic Value of P63 Expression in Muscle-Invasive Bladder Cancer and Association with Molecular Subtypes-Preliminary Report
Curr Issues Mol Biol. 2024 Mar 14;46(3):2456-2467. doi: 10.3390/cimb46030155.ABSTRACTThere is an ongoing need for biomarkers that could reliably predict the outcome of BC and that could guide the management of this disease. In this setting, we aimed to explore the prognostic value of the transcription factor P63 in patients with muscle-invasive bladder cancer (MIBC) having undergone radical cystectomy. The correlation between P63 expression and clinicopathological features (tumor stage, nodes involvement, patterns of muscularis propria invasion, papillary architecture, anaplasia, concomitant carcinoma in situ, lymphovascul...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Francesca Sanguedolce Ugo Giovanni Falagario Magda Zanelli Andrea Palicelli Maurizio Zizzo Stefano Ascani Simona Tortorella Gian Maria Busetto Angelo Cormio Giuseppe Carrieri Luigi Cormio Source Type: research

5-AZA Upregulates SOCS3 and PTPN6/SHP1, Inhibiting STAT3 and Potentiating the Effects of AG490 against Primary Effusion Lymphoma Cells
This study suggests that 5-AZA, by upregulating the expression level of SOCS3 and PTPN6/SHP1, reduced STAT3 activation and improved the outcome of treatment targeting this transcription factor in PEL cells.PMID:38534772 | PMC:PMC10968859 | DOI:10.3390/cimb46030156 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Michele Di Crosta Andrea Arena Rossella Benedetti Maria Saveria Gilardini Montani Mara Cirone Source Type: research

 N -3-Methylbutyl-benzisoselenazol-3(2H)-one Exerts Antifungal Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis
Curr Issues Mol Biol. 2024 Mar 14;46(3):2480-2496. doi: 10.3390/cimb46030157.ABSTRACTIn the present work, we evaluated the antifungal activities of two novel ebselen analogs, N-allyl-benzisoselenazol-3(2H)-one (N-allyl-bs) and N-3-methylbutylbenzisoselenazol-3(2H)-one (N-3mb-bs). Colorimetric and turbidity assays were performed to determine the minimum inhibitory concentration (MIC) of these compounds in S1 (fluconazole-sensitive) and S2 (fluconazole-resistant) strains of C. albicans. N-3mb-bs was more active than the N-allyl-bs compound. It is noteworthy that the concentration of N-3mb-bs observed to inhibit fungal growth...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Xiuyi Liang Agata J Pacu ła-Miszewska Richa Vartak Milankumar Prajapati Haiyan Zheng Caifeng Zhao Ganming Mao Ketankumar Patel Natalya U Fedosova Jacek Ścianowski Blase Billack Source Type: research

Exploring the Genomic Landscape of Bacillus paranthracis PUMB_17 as a Proficient Phosphatidylcholine-Specific Phospholipase C Producer
In this study, we present a strain of Bacillus paranthracis for the first time, demonstrating significant potential in the production of phosphatidylcholine-specific phospholipase C (PC-PLC). The investigation thoroughly examines the B. paranthracis PUMB_17 strain, focusing on the activity of PC-PLC and its purification process. Notably, the PUMB_17 strain displays extracellular PC-PLC production with high specific activity during the late exponential growth phase. To unravel the genetic makeup of PUMB_17, we employed nanopore-based whole-genome sequencing and subsequently conducted a detailed genome annotation. The genome...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Vesselin Baev Ivan Iliev Yordan Stefanov Marinela Tsankova Mariana Marhova Elena Apostolova Mariyana Gozmanova Galina Yahubyan Sonya Kostadinova Source Type: research

Complete Mitochondrial Genomes of Nedyopus patrioticus : New Insights into the Color Polymorphism of Millipedes
Curr Issues Mol Biol. 2024 Mar 15;46(3):2514-2527. doi: 10.3390/cimb46030159.ABSTRACTThere has been debate about whether individuals with different color phenotypes should have different taxonomic status. In order to determine whether the different color phenotypes of Nedyopus patrioticus require separate taxonomic status or are simply synonyms, here, the complete mitochondrial genomes (mitogenomes) of two different colored N. patrioticus, i.e., red N. patrioticus and white N. patrioticus, are presented. The two mitogenomes were 15,781 bp and 15,798 bp in length, respectively. Each mitogenome contained 13 PCGs, 19 tRNAs, 2...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Gaoji Zhang Tangjun Xu Yukun Chen Wei Xu Yinuo Wang Yuanyuan Li Fuyuan Zhu Hongyi Liu Honghua Ruan Source Type: research