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Role of Filamin A in Growth and Migration of Breast Cancer-Review
Curr Issues Mol Biol. 2024 Apr 17;46(4):3408-3423. doi: 10.3390/cimb46040214.ABSTRACTDespite ongoing research in the field of breast cancer, the morbidity rates indicate that the disease remains a significant challenge. While patients with primary tumors have relatively high survival rates, these chances significantly decrease once metastasis begins. Thus, exploring alternative approaches, such as targeting proteins overexpressed in malignancies, remains significant. Filamin A (FLNa), an actin-binding protein (ABP), is involved in various cellular processes, including cell migration, adhesion, proliferation, and DNA repair...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patryk Zawadka Wioletta Zieli ńska Maciej Gagat Magdalena Izdebska Source Type: research

Surface Engineering of Escherichia coli to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
This study explored a homologous protein production approach for displaying AppA on the cell surface of E. coli by engineering its outer membrane (OM) for extracellular expression. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of total bacterial lysates and immunofluorescence microscopy of non-permeabilized cells revealed protein expression, whereas activity assays using whole cells or OM fractions indicated functional enzyme display, as evidenced by consistent hydrolytic rates on typical substrates (i.e., p-nitrophenyl phosphate and phytic acid). Furthermore, the in vitro results obtained using a simp...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Patricia L A Mu ñoz-Muñoz Celina Ter án-Ramírez Rosa E Mares-Alejandre Ariana B M árquez-González Pablo A Madero-Ayala Samuel G Mel éndez-López Marco A Ramos-Ibarra Source Type: research

MicroRNAs in the Pathogenesis of Preeclampsia-A Case-Control In Silico Analysis
The objective of this secondary analysis was to elucidate the pathogenesis of PE by probing protein-protein interactions from in silico analysis of transcriptomes between PE and normal placenta from Gene Expression Omnibus (GSE149812). The pathogenesis of PE is apparently determined by associations of miRNA molecules and their target genes and the degree of changes in their expressions with irregularities in the functions of hemostasis, vascular systems, and inflammatory processes at the fetal-maternal interface. These irregularities ultimately lead to impaired placental growth and hypoxic injuries, generally manifesting a...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Ramanathan Kasimanickam Vanmathy Kasimanickam Source Type: research

Frequency of Gene Polymorphisms in Admixed Venezuelan Women with Recurrent Pregnancy Loss: Microsomal Epoxy Hydroxylase (rs1051740) and Enos (rs1799983)
This study aimed to assess the frequency of two SNPs present in two different enzymes involved in medical conditions observed during pregnancy. It is a case-control study. Microsomal epoxy hydrolase (mEPH) is involved in detoxifying xenobiotics, is present in the ovaries, and is hormonally regulated. The endothelial nitric oxide synthase (NOS3) that forms nitric is involved in vascular tone. Two SNPs, rs1051740 (mEPH) and rs1799983 (NOS3), were assessed. The study included 50 controls and 63 primary RPL patients. The frequency of mutated alleles in both SNPs was significantly higher in patients (p < 0.05). Double-mutate...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Mar ía Johanna Peña Claudia Valentina De Sanctis Juan Bautista De Sanctis Jenny Valentina Garmendia Source Type: research

Kahweol Inhibits Pro-Inflammatory Cytokines and Chemokines in Tumor Necrosis Factor- α/Interferon-γ-Stimulated Human Keratinocyte HaCaT Cells
Curr Issues Mol Biol. 2024 Apr 18;46(4):3470-3483. doi: 10.3390/cimb46040218.ABSTRACTAtopic dermatitis (AD), marked by intense itching and eczema-like lesions, is a globally increasing chronic skin inflammation. Kahweol, a diterpene that naturally occurs in coffee beans, boasts anti-inflammatory, antioxidative, and anti-cancer properties. This research explores the anti-inflammatory action of kahweol on HaCaT human keratinocytes stimulated by tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), focusing on key signal transduction pathways. Our results demonstrate that kahweol markedly reduces the production of IL-...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Ye Jin Kwon Hyun Hee Kwon Jaechan Leem Yoon Young Jang Source Type: research

Rhoa/ROCK, mTOR and Secretome-Based Treatments for Ischemic Stroke: New Perspectives
Curr Issues Mol Biol. 2024 Apr 18;46(4):3484-3501. doi: 10.3390/cimb46040219.ABSTRACTIschemic stroke triggers a complex cascade of cellular and molecular events leading to neuronal damage and tissue injury. This review explores the potential therapeutic avenues targeting cellular signaling pathways implicated in stroke pathophysiology. Specifically, it focuses on the articles that highlight the roles of RhoA/ROCK and mTOR signaling pathways in ischemic brain injury and their therapeutic implications. The RhoA/ROCK pathway modulates various cellular processes, including cytoskeletal dynamics and inflammation, while mTOR sig...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Elena Anca Pino șanu Denisa P îrșcoveanu Carmen Valeria Albu Emilia Burada Andrei P îrvu Roxana Surugiu Raluca Elena Sandu Alina Florina Serb Source Type: research

Inflammasome Molecular Insights in Autoimmune Diseases
Curr Issues Mol Biol. 2024 Apr 18;46(4):3502-3532. doi: 10.3390/cimb46040220.ABSTRACTAutoimmune diseases (AIDs) emerge due to an irregular immune response towards self- and non-self-antigens. Inflammation commonly accompanies these conditions, with inflammatory factors and inflammasomes playing pivotal roles in their progression. Key concepts in molecular biology, inflammation, and molecular mimicry are crucial to understanding AID development. Exposure to foreign antigens can cause inflammation, potentially leading to AIDs through molecular mimicry triggered by cross-reactive epitopes. Molecular mimicry emerges as a key m...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Monica Neam țu Veronica Bild Alexandru Vasincu Oana Dana Arcan Delia Bulea Daniela-Carmen Ababei R ăzvan-Nicolae Rusu Ioana Macadan Ana Maria Sciuc ă Andrei Neam țu Source Type: research

CCN1-Mediated Signaling in Placental Villous Tissues after SARS-CoV-2 Infection in Term Pregnant Women: Implications for Dysregulated Angiogenesis
This study aimed to explore placental pathology alterations and angiogenic factor levels in term pregnant women after SARS-CoV-2 infection in a retrospective single-center study. Additionally, we investigated the role and underlying mechanism of the vascular inflammation-promoting, cysteine-rich protein 61 (CYR61/CCN1) in this context. All analyses were performed in term pregnant women infected with or without SARS-CoV-2. The sFlt-1, PlGF, and sEng serum levels were quantified using ELISA. Placental protein expressions were examined by immunoblot and immunostaining. Additionally, the effect of CCN1 protein on SGHPL-5 troph...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Yuyang Ma Liyan Duan Beatrix Reisch Rainer Kimmig Antonella Iannaccone Alexandra Gellhaus Source Type: research

Multi-Omics Integration for Liver Cancer Using Regression Analysis
This report elucidates how the integration of comprehensive data on methylation, gene expression, and copy number variations can effectively facilitate the unsupervised clustering of cancer samples. We have identified regressors that can effectively classify tumor and normal samples with an optimal integration of RNA sequencing, DNA methylation, and copy number variation while also achieving significant p-values. Further, these regressors were trained using linear and logistic regression with k-means clustering. For comparison, we employed autoencoder- and stacking-based omics integration and computed silhouette scores to ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Aditya Raj Ruben C Petreaca Golrokh Mirzaei Source Type: research

Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
Curr Issues Mol Biol. 2024 Apr 19;46(4):3563-3578. doi: 10.3390/cimb46040223.ABSTRACTPolycaprolactone (PCL) scaffolds have demonstrated an effectiveness in articular cartilage regeneration due to their biomechanical properties. On the other hand, alginate hydrogels generate a 3D environment with great chondrogenic potential. Our aim is to generate a mixed PCL/alginate scaffold that combines the chondrogenic properties of the two biomaterials. Porous PCL scaffolds were manufactured using a modified salt-leaching method and embedded in a culture medium or alginate in the presence or absence of chondrocytes. The chondrogenic ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Lara Mili án Mar ía Oliver-Ferrándiz Ignacio Peregr ín Mar ía Sancho-Tello Jos é Javier Martín-de-Llano Cristina Mart ínez-Ramos Carmen Carda Manuel Mata Source Type: research

Association between KRAS and PIK3CA Mutations and Progesterone Resistance in Endometriotic Epithelial Cell Line
In this study, we investigated the effects of these mutations on progesterone resistance. Since the HMOsisEC10 had suppressed progesterone receptor (PR) expression, we transduced PR-B to HMOsisEc10 cell lines including KRAS mutant and PIK3CA mutant cell lines. We conducted a migration assay, invasion assay, and MTT assay using dienogest and medroxyprogestrone acetate. All cell lines showed progesterone sensitivity with or without mutations. Regarding inflammatory factors, real-time quantitative RT-PCR revealed that the KRAS mutation cell line exhibited no suppression of Cox-2 and mPGES-1 on progesterone treatment, whereas ...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Kosuke Kanno Kentaro Nakayama Sultana Razia Sohel Hasibul Islam Zahan Umme Farzana Shahataj Begum Sonia Hitomi Yamashita Masako Ishikawa Tomoka Ishibashi Kayo Imamura Tohru Kiyono Satoru Kyo Source Type: research

Microbiome Sex-Related Diversity in Non-Muscle-Invasive Urothelial Bladder Cancer
Curr Issues Mol Biol. 2024 Apr 19;46(4):3595-3609. doi: 10.3390/cimb46040225.ABSTRACTSex-specific discrepancies in bladder cancer (BCa) are reported, and new studies imply that microbiome may partially explain the diversity. We aim to provide characterization of the bladder microbiome in both sexes diagnosed with non-muscle-invasive BCa with specific insight into cancer grade. In our study, 16S rRNA next-generation sequencing was performed on midstream urine, bladder tumor sample, and healthy-appearing bladder mucosa. Bacterial DNA was isolated using QIAamp Viral RNA Mini Kit. Metagenomic analysis was performed using hyper...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Konrad Bilski Natalia Żeber-Lubecka Maria Kulecka Michalina D ąbrowska Aneta Ba łabas Jerzy Ostrowski Aleksandra Dobruch Jakub Dobruch Source Type: research

Correlation between the RNA Expression and the DNA Methylation of Estrogen Receptor Genes in Normal and Malignant Human Tissues
Curr Issues Mol Biol. 2024 Apr 19;46(4):3610-3625. doi: 10.3390/cimb46040226.ABSTRACTEstrogen plays a multifaceted function in humans via interacting with the estrogen receptors ERα, ERβ, and G protein-coupled estrogen receptor 1 (GPER1). Previous research has predominantly concentrated on elucidating the signaling route of estrogen. However, the comprehensive understanding of the expression profile and control of these estrogen receptors in various human tissues is not well known. In the present study, the RNA levels of estrogen receptors in various normal and malignant human tissues were retrieved from the human protei...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Ju Rong Xiaojun Xie Yongdong Niu Zhongjing Su Source Type: research

Phytocannabinoids CBD, CBG, and their Derivatives CBD-HQ and CBG-A Induced In Vitro Cytotoxicity in 2D and 3D Colon Cancer Cell Models
In this study, we tested the anticancer potential of selected cannabinoids CBD (cannabidiol) and its quinone derivative CBD-HQ (cannabidiol hydroquinone), CBG (cannabigerol) and its acid derivative CBG-A (cannabigerolic acid), as well as a combination of CBD+CBG on the colon cancer cell line SW-620. The MTT assay was used to determine the cannabinoids' ability to induce colon cancer cell death. All cannabinoids were cytotoxic at the lowest concentration (3 μg/mL). The half maximal inhibitory concentration (IC50) ranged from 3.90 to 8.24 μg/mL, depending on the substance. Cytotoxicity was confirmed in a 3D spheroidal cell...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Dorota B ęben Oliwia Siwiela Anna Szyjka Micha ł Graczyk Daniel Rzepka Ewa Barg Helena Moreira Source Type: research

The Role of Alarmins in the Pathogenesis of Rheumatoid Arthritis, Osteoarthritis, and Psoriasis
Curr Issues Mol Biol. 2024 Apr 19;46(4):3640-3675. doi: 10.3390/cimb46040228.ABSTRACTAlarmins are immune-activating factors released after cellular injury or death. By secreting alarmins, cells can interact with immune cells and induce a variety of inflammatory responses. The broad family of alarmins involves several members, such as high-mobility group box 1, S100 proteins, interleukin-33, and heat shock proteins, among others. Studies have found that the concentrations and expression profiles of alarmins are altered in immune-mediated diseases. Furthermore, they are involved in the pathogenesis of inflammatory conditions...
Source: Current Issues in Molecular Biology - April 26, 2024 Category: Molecular Biology Authors: Kajetan Kie łbowski Wiktoria Sta ńska Estera Bakinowska Marcin Rusi ński Andrzej Pawlik Source Type: research