Metabolic Interplay in the Tumor Microenvironment: Implications for Immune Function and Anticancer Response
Curr Issues Mol Biol. 2023 Dec 5;45(12):9753-9767. doi: 10.3390/cimb45120609.ABSTRACTMalignant tumors exhibit rapid growth and high metabolic rates, similar to embryonic stem cells, and depend on aerobic glycolysis, known as the "Warburg effect". This understanding has enabled the use of radiolabeled glucose analogs in tumor staging and therapeutic response assessment via PET scans. Traditional treatments like chemotherapy and radiotherapy target rapidly dividing cells, causing significant toxicity. Despite immunotherapy's impact on solid tumor treatment, gaps remain, leading to research on cancer cell evasion of immune re...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Reem Youssef Rohan Maniar Jaffar Khan Hector Mesa Source Type: research

DNA Methylation of the IL-17A Gene Promoter Is Associated with Subclinical Atherosclerosis and Coronary Artery Disease: The Genetics of Atherosclerotic Disease Mexican Study
Curr Issues Mol Biol. 2023 Dec 5;45(12):9768-9777. doi: 10.3390/cimb45120610.ABSTRACTThe interleukin-17 (IL-17) has a crucial role during inflammation and has been associated with cardiovascular diseases, but its role in epigenetics is still poorly understood. Therefore, the aim of this study was to evaluate the DNA methylation status of the IL-17A gene promoter to establish whether it may represent a risk factor for subclinical atherosclerosis (SA) or clinical coronary artery disease (CAD). We included 38 patients with premature CAD (pCAD), 48 individuals with SA, and 43 healthy controls. Methylation in the CpG region of ...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Nonanzit P érez-Hernández Rosalinda Posadas-S ánchez Gilberto Vargas-Alarc ón Óscar Pérez-Méndez Mar ía Luna-Luna Jos é Manuel Rodríguez-Pérez Source Type: research

Impact of Hypoxia-Induced miR-210 on Pancreatic Cancer
Curr Issues Mol Biol. 2023 Dec 5;45(12):9778-9792. doi: 10.3390/cimb45120611.ABSTRACTPancreatic cancer (PC) poses significant clinical challenges, with late-stage diagnosis and limited therapeutic options contributing to its dismal prognosis. A hallmark feature of PC is the presence of a profoundly hypoxic tumour microenvironment, resulting from various factors such as fibrotic stroma, rapid tumour cell proliferation, and poor vascularization. Hypoxia plays a crucial role in promoting aggressive cancer behaviour, therapeutic resistance, and immunosuppression. Previous studies have explored the molecular mechanisms behind h...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Mutian Lian Maria Mortoglou Pinar Uysal-Onganer Source Type: research

Cationic Porphyrins as Antimicrobial and Antiviral Agents in Photodynamic Therapy
Curr Issues Mol Biol. 2023 Dec 6;45(12):9793-9822. doi: 10.3390/cimb45120612.ABSTRACTAntimicrobial photodynamic therapy (APDT) has received a great deal of attention due to its unique ability to kill all currently known classes of microorganisms. To date, infectious diseases caused by bacteria and viruses are one of the main sources of high mortality, mass epidemics and global pandemics among humans. Every year, the emergence of three to four previously unknown species of viruses dangerous to humans is recorded, totaling more than 2/3 of all newly discovered human pathogens. The emergence of bacteria with multidrug resista...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Inga O Savelyeva Kseniya A Zhdanova Margarita A Gradova Oleg V Gradov Natal'ya A Bragina Source Type: research

Amelioration of Motor Performance and Nigrostriatal Dopamine Cell Volume Using a Novel Far-Infrared Ceramic Blanket in an A53T Alpha-Synuclein Transgenic Parkinson's Disease Mouse Model
Curr Issues Mol Biol. 2023 Dec 6;45(12):9823-9837. doi: 10.3390/cimb45120613.ABSTRACTWe had attended a Parkinson's Disease (PD) patient for a non-healing wound who reported a marked decrease in his hand tremor and freezing of gait when his wound was exposed to a ceramic far-field infrared (cFIR) blanket. PD is the most frequent motor disorder and the second most frequent neurodegenerative disease after Alzheimer's Disease (AD). The tremor, rigidity, and slowness of movement associated with Parkinson's disease (PD) affect up to 10 million people throughout the world, and the major contributing factor to the pathogenesis of ...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Frederick Robert Carrick Luis Sebastian Alexis Valerio Hernandez Kiminobu Sugaya Source Type: research

Retinol-Binding Protein 4 and Visfatin Levels in Patients with Periodontitis and Obesity/Overweight: A Systematic Review and Meta-Analysis
Curr Issues Mol Biol. 2023 Dec 7;45(12):9838-9850. doi: 10.3390/cimb45120614.ABSTRACTPrior studies demonstrated an equivocal conclusion about the association between the level of retinol-binding protein 4 (RBP4)/visfatin and periodontitis patients with obesity. The aim of our study (Prospero ID: CRD42023469058) was to systematically review the available articles linking the biofluid levels of RBP4/visfatin to the comorbidity of periodontitis and obesity. Clinical trials were screened in accordance with specific inclusion criteria from seven databases up to November 2023. A quality assessment was performed with the Newcastl...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Yuwei Zhang Ru Jia Yifei Zhang Rui Zou Lin Niu Shaojie Dong Source Type: research

Exosomal Cargo in Ovarian Cancer Dissemination
Curr Issues Mol Biol. 2023 Dec 7;45(12):9851-9867. doi: 10.3390/cimb45120615.ABSTRACTOvarian cancer (OC) has the highest mortality rate among all gynecologic cancers and is characterized by early peritoneal spread. The growth and development of OC are associated with the formation of ascitic fluid, creating a unique tumor microenvironment. Understanding the mechanisms of tumor progression is crucial in identifying new diagnostic biomarkers and developing novel therapeutic strategies. Exosomes, lipid bilayer vesicles measuring 30-150 nm in size, are known to establish a crucial link between malignant cells and their microen...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Ekaterina Dzhugashvili Svetlana Tamkovich Source Type: research

Hydroxycitric Acid Alleviated Lung Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Ferroptosis through the Hif-1 α Pathway
Curr Issues Mol Biol. 2023 Dec 8;45(12):9868-9886. doi: 10.3390/cimb45120616.ABSTRACTLung ischemia-reperfusion injury (LIRI) is a prevalent occurrence in various pulmonary diseases and surgical procedures, including lung resections and transplantation. LIRI can result in systemic hypoxemia and multi-organ failure. Hydroxycitric acid (HCA), the primary acid present in the peel of Garcinia cambogia, exhibits anti-inflammatory, antioxidant, and anticancer properties. However, the effects of HCA on LIRI remain unknown. To investigate the impact of HCA on LIRI in mice, the mice were randomly divided into four groups: the contro...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Zi-Long Lu Cong-Kuan Song Shi-Shi Zou Shi-Ze Pan Kai Lai Ning Li Qing Geng Source Type: research

Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus
Curr Issues Mol Biol. 2023 Dec 8;45(12):9887-9903. doi: 10.3390/cimb45120617.ABSTRACTAnti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Tatiana S Novikova Evgeny A Ermakov Elena V Kostina Alexander N Sinyakov Alexey E Sizikov Georgy A Nevinsky Valentina N Buneva Source Type: research

A Systematic Review of Lipid-Focused Cardiovascular Disease Research: Trends and Opportunities
Curr Issues Mol Biol. 2023 Dec 9;45(12):9904-9916. doi: 10.3390/cimb45120618.ABSTRACTLipids are important modifiers of protein function, particularly as parts of lipoproteins, which transport lipophilic substances and mediate cellular uptake of circulating lipids. As such, lipids are of particular interest as blood biological markers for cardiovascular disease (CVD) as well as for conditions linked to CVD such as atherosclerosis, diabetes mellitus, obesity and dietary states. Notably, lipid research is particularly well developed in the context of CVD because of the relevance and multiple causes and risk factors of CVD. Th...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Uchenna Alex Anyaegbunam Piyush More Jean-Fred Fontaine Vincent Ten Cate Katrin Bauer Ute Distler Elisa Araldi Laura Bindila Philipp Wild Miguel A Andrade-Navarro Source Type: research

A Novel Mutation in Sacsin, p.Val1335IIe, May Cause Late-Onset Sacsinopathy Due to Haploinsufficiency
In this study, a novel SACS mutation (p.Val1335IIe, Heterozygous) was identified in a Korean patient in their 50s with late-onset ARSACS characterized by cerebellar ataxia and spasticity without peripheral neuropathy. The mutation was confirmed via whole exome sequencing and Sanger sequencing and was predicted to likely cause disease using prediction software. RT-PCR and ELISA showed decreased SACS mRNA expression and sacsin protein concentrations in the proband, supporting its implications in diseases with pathogenicity and reduced chaperone function from haploinsufficiency. Our results revealed the pathogenicity of the S...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Danyeong Kim Nayoung Ryoo Young Ho Park Eva Bagyinszky Seong Soo Alexander An SangYun Kim Source Type: research

Lumbricus Extract Prevents LPS-Induced Inflammatory Activation of BV2 Microglia and Glutamate-Induced Hippocampal HT22 Cell Death by Suppressing MAPK/NF- κB/NLRP3 Signaling and Oxidative Stress
Curr Issues Mol Biol. 2023 Dec 11;45(12):9926-9942. doi: 10.3390/cimb45120620.ABSTRACTMicroglia-induced inflammatory signaling and neuronal oxidative stress are mutually reinforcing processes central to the pathogenesis of neurodegenerative diseases. Recent studies have shown that extracts of dried Pheretima aspergillum (Lumbricus) can inhibit tissue fibrosis, mitochondrial damage, and asthma. However, the effects of Lumbricus extracts on neuroinflammation and neuronal damage have not been previously studied. Therefore, to evaluate the therapeutic potential of Lumbricus extract for neurodegenerative diseases, the current s...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: You-Chang Oh Yun Hee Jeong Hye Jin Yang Wei Li Jin Yeul Ma Source Type: research

Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers
This study provides novel insights into the mechanisms of enhanced UV-B radiation that regulate the anthocyanin biosynthesis in potato tubers.PMID:38132467 | PMC:PMC10742819 | DOI:10.3390/cimb45120621 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Lingyan Cui Maoxing Li Xing Zhang Zongming Guo Kaifeng Li Yuhan Shi Qiong Wang Huachun Guo Source Type: research

The Influence of Betulin Derivatives EB5 and ECH147 on the Expression of Selected TGF β Superfamily Genes, < em > TGF β1 < /em > , < em > GDF15 < /em > and < em > BMP2 < /em > , in Renal Proximal Tubule Epithelial Cells
The objective of our study was to assess the influence of the betulin derivatives 28-propynylobetulin (EB5) and 29-diethoxyphosphoryl-28-propynylobetulin (ECH147) on the expression of TGFβ1, BMP2 and GDF15 in renal proximal tubule epithelial cells (RPTECs) cultured in vitro. The changes in mRNA expression and copy numbers were assessed using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) and the standard curve method, respectively. An enzyme-linked immunosorbent assay (ELISA) was used to evaluate the effect of the betulin derivatives on the protein concentration in the culture media's sup...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Sebastian Kubica Justyna Szota-Czy ż Barbara Strza łka-Mrozik Jolanta Adamska Ewa B ębenek Elwira Chrobak Joanna Magdalena Gola Source Type: research

Advocacy of Precision Allergy Molecular Diagnosis in Decision Making for the Eligibility of Customized Allergen Immunotherapy
Curr Issues Mol Biol. 2023 Dec 12;45(12):9976-9984. doi: 10.3390/cimb45120623.ABSTRACTAllergen immunotherapy (AIT) with aeroallergens is the only disease-modifying treatment for patients with different allergic conditions. Despite the effectiveness of AIT having been proven in both randomized controlled trials and real-world studies, it remains underused in less than 10% of subjects with allergic rhinitis (AR) and/or asthma (A). We aimed to determine the current eligibility for house dust mite (HDM) AIT by means of a precision allergy molecular diagnosis (PAMD@) model in a selected cohort of youngsters with different aller...
Source: Current Issues in Molecular Biology - December 22, 2023 Category: Molecular Biology Authors: Ruperto Gonz ález-Pérez Paloma Poza-Guedes Fernando Pineda Inmaculada S ánchez-Machín Source Type: research