Molecular Genotyping of < em > Giardia duodenalis < /em > in Humans in the Yazd County, Central of Iran
CONCLUSION: Assemblages A and B are the most prevalent in Central Iran. The molecular identification of G. duodenalis isolates from animals and implementing control programs.PMID:38654947 | PMC:PMC11033543 | DOI:10.18502/ijpa.v19i1.15216 (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - April 24, 2024 Category: Parasitology Authors: Elaheh Hezardastan Ali Fattahi Bafghi Gilda Eslami Mahmoud Vakili Source Type: research
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis
This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development.PMID:38656038 | DOI:10.1590/S1678-9946202466022 (Source: Revista do Instituto de Medicina Tropical de Sao Paulo)
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - April 24, 2024 Category: Tropical Medicine Authors: Jonaia Novaes da Costa Jones Anderson Monteiro Siqueira Dielle Monteiro Teixeira Patr ícia Dos Santos Lobo Sylvia de F átima Dos Santos Guerra Isadora Monteiro Souza Bruna Trindade Moreira Cardoso Luana Silva Soares Farias Hugo Reis Resque Yvone Benchim Source Type: research
YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition
by Mai Ikei, Ryoji Miyazaki, Keigo Monden, Yusuke Naito, Azusa Takeuchi, Yutaro S. Takahashi, Yoshiki Tanaka, Keina Murata, Takaharu Mori, Muneyoshi Ichikawa, Tomoya Tsukazaki
Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) inEscherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, rema...
Source: PLoS Biology: Archived Table of Contents - April 24, 2024 Category: Biology Authors: Mai Ikei Source Type: research
Construction of the ETECFinder database for the characterization of enterotoxigenic Escherichia coli (ETEC) and revision of the VirulenceFinder web tool at the CGE website
Journal of Clinical Microbiology, Ahead of Print. (Source: Journal of Clinical Microbiology)
Source: Journal of Clinical Microbiology - April 24, 2024 Category: Microbiology Authors: Flemming ScheutzCamilla Hald NielsenAstrid von Mentzer1The International Escherichia and Klebsiella Centre, Statens Serum Institut, Copenhagen, Denmark2Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark3Department of Source Type: research
Perforated Appendicitis in Children: Management, Microbiology, and Antibiotic Stewardship
AbstractAlthough appendicitis has been described for more than 300 years, its optimal management remains a topic of active investigation. Acute appendicitis is the most common cause of peritonitis in children, and rates of perforated appendicitis are much higher in children than in adults. Increased risk for perforated appendicitis in children is related to a delay in diagnosis due to age, size, access to care, and more. Surgical options include immediate appendectomy versus nonoperative management with intravenous antibiotics ± a drainage procedure, with a subsequent interval appendectomy. Microbiota of perforated append...
Source: Pediatric Drugs - April 24, 2024 Category: Pediatrics Source Type: research
Molecules, Vol. 29, Pages 1941: Microbiological and Physicochemical Evaluation of Hydroxypropyl Methylcellulose (HPMC) and Propolis Film Coatings for Cheese Preservation
This study aimed to evaluate the efficacy of hydroxypropyl methylcellulose (HPMC) and propolis film as protective coatings for cheese. For this, microbiological analyses were carried out over the cheese&rsquo; ripening period, focusing on total mesophilic bacteria, yeasts and moulds, lactic acid bacteria, total coliforms, Escherichia coli, and Enterobacteriaceae. Physicochemical parameters (pH, water activity, colour, phenolic compounds content) were also evaluated. The statistical analysis (conducted using ANOVA and PERMANOVA) showed a significant interaction term between the HPMC film and propolis (factor 1) and ...
Source: Molecules - April 24, 2024 Category: Chemistry Authors: Vanessa B. Paula Lu ís G. Dias Let ícia M. Estevinho Tags: Article Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Perforated Appendicitis in Children: Management, Microbiology, and Antibiotic Stewardship
Paediatr Drugs. 2024 Apr 24. doi: 10.1007/s40272-024-00630-0. Online ahead of print.ABSTRACTAlthough appendicitis has been described for more than 300 years, its optimal management remains a topic of active investigation. Acute appendicitis is the most common cause of peritonitis in children, and rates of perforated appendicitis are much higher in children than in adults. Increased risk for perforated appendicitis in children is related to a delay in diagnosis due to age, size, access to care, and more. Surgical options include immediate appendectomy versus nonoperative management with intravenous antibiotics ± a drainage...
Source: Paediatric Drugs - April 23, 2024 Category: Pediatrics Authors: Katherine B Snyder Catherine J Hunter Christie L Buonpane Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Paper-based loop-mediated isothermal amplification and CRISPR integrated platform for on-site nucleic acid testing of pathogens
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates the need for thermal cycling, resulting in simplified instrumentation, and the CRISPR-associated protein (Cas 12a) system eliminates false positive signals from LAMP products, resulting in highly selective and sensitive assays. We optimized the assay to perform both amplification and detection entir...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Anindita Sen Manaswini Masetty Sasanka Weerakoon Calum Morris Jagjit S Yadav Senu Apewokin Jennifer Trannguyen Murray Broom Aashish Priye Source Type: research
Greenspace and human microbiota: A systematic review
CONCLUSION: The current evidence suggests that greenspace exposure may diversify gut and skin microbiota and alter their composition to healthier profiles. These findings would be helpful in uncovering the potential mechanisms underlying greenspace and human health and in promoting a healthier profile of human microbiota.PMID:38653130 | DOI:10.1016/j.envint.2024.108662 (Source: Environment International)
Source: Environment International - April 23, 2024 Category: Environmental Health Authors: Yi-Dan Zhang Gang-Long Zhou Lu Wang Matthew H E M Browning Iana Markevych Joachim Heinrich Luke D Knibbs Tianyu Zhao Yizhen Ding Shi Chen Kang-Kang Liu Payam Dadvand Guang-Hui Dong Bo-Yi Yang Source Type: research
Application of Mono and Trinuclear Cyclometalated Iridium (III) Complexes in Differential Bacterial Imaging and Antimicrobial Photodynamic therapy
Chemistry. 2024 Apr 23:e202400646. doi: 10.1002/chem.202400646. Online ahead of print.ABSTRACTThe application of transition metal complexes for antimicrobial photodynamic therapy (PDT) has emerged as an attractive alternative in mitigating a broad range of bacterial pathogens, including multidrug-resistant pathogens. In view of their photostability, long excited-state lifetimes, and tunable emission properties, transition metal complexes also contribute as bioimaging agents. In the present work, we designed mono and trinuclear cyclometalated iridium(III) complexes to explore their imaging application and antibacterial pote...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: Bishnu Das Prakash Biswas Amirul Islam Mallick Parna Gupta Source Type: research
Efficient Synthetic Access to Stable Isotope Labelled Pseudouridine Phosphoramidites for RNA NMR Spectroscopy
Chemistry. 2024 Apr 23:e202401193. doi: 10.1002/chem.202401193. Online ahead of print.ABSTRACTHere we report the efficient synthetic access to 13C/15N-labelled pseudouridine phosphoramidites, which were incorporated into a binary H/ACA box guide RNA/product complex comprising 77 nucleotides (nts) in total and into a 75 nt E. coli tRNAGly. The stable isotope (SI) labelled pseudouridines were produced via a highly efficient chemo-enzymatic synthesis. 13C/15N labelled uracils were produced via chemical synthesis and enzymatically converted to 5'-monophosphate pseudouridine (ΨMP) by using YeiN, a Ψ-5'-monophosphate C-glycosi...
Source: Chemistry - April 23, 2024 Category: Chemistry Authors: David Gl änzer Martin Pfeiffer Andrej Ribar Ricarda Zeindl Martin Tollinger Bernd Nidetzky Christoph Kreutz Source Type: research
