Isolation and Genotypic Characterization of Toxoplasma gondii Based on GRA6 Gene from Environmental Soil Samples in Mazandaran Province, North of Iran.
Authors: Haghparast-Kenari B, Sarvi S, Sharif M, Ahmadpour E, Hosseini SA, Daryani A Abstract Background: Soil is one of the environmental sources of Toxoplasma gondii oocysts. The other hand, genotype of the parasite is one of the important factors for its pathogenicity. Due to the importance of toxoplasmosis on public health, this study aimed to isolation and genotyping of T. gondii in environmental soil samples of Mazandaran Province, north of Iran. Methods: Overall, 192 soil samples were collected from different areas in Mazandaran Province from Apr to Sep 2014. The flotation method was used for recovering ...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The Effect of Alpha-Tocopherol on the Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Beta Genes in Three Developmental Stages of Echinococcus granulosus.
Conclusion: Administration of alpha-tocopherol to different developmental stages of E. granulosus significantly enhanced EGFR and TGF-beta expression in the parasite. Both oxidant and non-oxidant activities of alpha-tocopherol could explain the study findings. Overexpression of the genes could in turn enhance growth factor effects and facilitates the viability of the parasite. PMID: 32595706 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis.
Conclusion: Our data indicating on existence of significant number of asymptomatic reservoirs that assists in prolonged endemicity of the disease. On the other hand, the molecular methods are better alternatives for microscopy especially for monitoring of asymptomatic cases of malaria. PMID: 32595707 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Serological Evaluation of Anti-Toxoplasma gondii Antibodies in Patients with Acute Leukemia and Lymphoma through Chemotherapy.
Conclusion: Performing specialized tests to diagnose toxoplasma infection before starting treatment, in immunodeficiency patients who undergo chemotherapy, is necessary; therefore, these tests should be considered in therapeutic protocols. PMID: 32595708 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Prevalence and Viability of Toxocara spp. Eggs in Soil of Public Parks in Northwestern Mexico.
Authors: Vargas Nava AI, Castro Del Campo N, EnrÍquez Verdugo I, Portillo Loera JJ, Barraza Tizoc CL, Gaxiola Camacho SM Abstract Background: Toxocara spp. is a zoonotic parasite that can infect human; children are the largest group at risk of infection. Therefore, this study aimed to determine the prevalence and viability of Toxocara spp. eggs in the soil of public parks. Methods: Overall, 1180 soil samples from 236 public parks in four sectors of the city of Culiacan were collected at random, between Jun and Dec, 2013. The presence of Toxocara spp. eggs was determined by light microscopy using a centri...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran.
Authors: Sepahvand A, Hosseini-Safa A, Yousofi HA, Tajedini MH, Pahlavan Gharehbabah R, Pestehchian N Abstract Background: We aimed at genotyping and evaluating the predominance of G. duodenalis assemblages isolated from patients referred to medical laboratories in Khorramabad, Iran from Nov 2015 to Sep 2016. Hence, the development of a cost-effective HRM approach to determine genotypes of G. duodenalis based on the triosephosphate isomerase (tpi) gene was examined and the genotyping results with and without diarrhea was compared. Methods: Seventy G. duodenalis positive fecal samples were collected. A microscop...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Clinico-Hematological Findings of Acute Pediatric Visceral Leishmaniasis Referred to the Northeast of Iran during 2005-2015.
Conclusion: Bone marrow/splenic aspiration still remains the gold standard test despite its risk and pain for patients. PMID: 32595711 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Arthrospira maxima Paradoxical Effect on Trypanosoma cruzi Infection.
Conclusion: A. maxima administration overactive the immune system, decreasing the parasitemia, but causing a severe tissue damage. Then, this nutraceutical has a paradoxical effect on intracellular parasitic infections such as Chagas disease. PMID: 32595712 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The Effects of pH and Temperature on Cysteine Protease (Cathepsin B) Activity in Miracidia and Eggs of Fasciola hepatica.
Conclusion: Upon the importance of pH and temperature in the life cycle of F. hepatica, the current findings can be used for induction of some modifications in pH and preventing the activity of the enzymes for decrement of the efficacy of miracidia penetration into the intermediate snails and egg hatching of this zoonotic parasite. PMID: 32595713 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Helminthes in Feral Raccoon (Procyon lotor) as an Alien Species in Iran.
Conclusion: Most of the raccoons were infected with S. procyonis. The public health importance of zoonotic parasites transmittable through raccoons, the rapid control and decrease of raccoon populations and their distribution in Iran are also discussed. PMID: 32595714 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Survey of Toxocara eggs on Dog Hair as a Potential Transmission Route in Human Toxocariasis in Northeastern Iran.
Conclusion: Human exposure to the hair of dogs, may be significant risk factor for infection and regular anthelmintic treatment, hygiene of animals and public education of the importance of dogs are recommended to prevent human toxocariasis. PMID: 32595715 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Serological Survey of Neospora caninum and Toxoplasma gondii Co-Infection in Rodents in Northwestern Iran.
Authors: Nazari N, Shojaee S, Salimi M, Mohebali M, Ahmadifard N, Hamzavi Y, Zarei Z, Farahmand-Rad R, Bozorgomid A, Heydarian P Abstract Background: Our knowledge of the epidemiology of rodents' parasitic agents in Iran is scarce, although some of these pathogens play an important role in human and veterinary medicine, such as Toxoplasma gondii and Neospora caninum. The purpose of this study was to determine the seroprevalence of Toxoplasma gondii and Neospora caninum in rodents of northwestern Iran between Mar and Dec 2015. Methods: Overall, 157 serum samples from rodents (101 Meriones persicus, 41 Mus muscul...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran.
Conclusion: Genotypes of E. granulosus in human samples in Hamadan Province are G1 and G3 and these findings are proved by phylogenic analysis. PMID: 32595717 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Ectoparasites and Endoparasites of New Zealand White Rabbits from North West of Iran.
Conclusion: Both domestic and wild rabbits are a potential source of human parasitic zoonosis, and strict hygienic practices are recommended during and after handling rabbits or in case of exposure to their feces. PMID: 32595718 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Acanthamoeba Keratitis and Acanthamoeba Conjunctivitis: A Case Report.
Authors: Alver O, Baykara M, YÜrÜk M, TÜzemen NÜ Abstract Acanthamoeba species are vision-threatening agents by causing cornea infections known as Acanthamoeba keratitis. A 5 year-old kid with the complaints of erythema, eyelid edema, inflammation, limitation of eye movements in the right eye, and having no history of wearing contact lenses or trauma, was diagnosed of Acanthamoeba conjunctivitis through laboratory examinations in the Ophthalmology clinic. The visual sharpness of the patient improved after the treatment. A 44 year-old female patient suffering from pain, stinging, irritation, and ...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Disseminated Leishmaniasis Due to Using Immunosuppression Drugs: A Case Report.
In this report, we will present the symptoms and manifestations of this disease to reduce late detection and exacerbating factors. The patient was a three-year-old girl from Tehran, Iran who had ascites and hepatomegaly. When she was 9 month-old, she was diagnosed as autoimmune hepatitis after liver biopsy and she was treated with immunosuppressive drugs (Azathioprine, prednisolone, and cyclosporine) for 22 months, but later she suffered from fever, pancytopenia, and hepatosplenomegaly. Then a bone marrow biopsy was done for her. There was a large amount of Leishman body in her bone marrow and treatment for Kala-azar was s...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

First Report of Joyeuxiella spp. Infection in Eurasian Lynx and Its Histopathology Study from Iran: A Case Report.
Authors: Hosseini SM, Moshrefi AH, Esfandiyari A, Youssefi MR, Nassiri A Abstract According to the last information of IUCN Red List, Eurasian lynx has been endangered since 2010. The population of this animal is heavily affected by infectious parasites. Two adult Eurasian lynx (one male and one female) were illegally hunted and found dead in Parvar National Park, Semnan Province, Iran. After the autopsy, the tissue and parasite samples were collected from gastrointestinal tract and transferred to 70% alcohol. Samples were recovered and identified as Joyeuxiella spp. Sonsino, 1889. Tissue samples were taken from th...
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Is It Necessary to Terminate Pregnancy before Hydatid Cyst Surgery?
Authors: Shahraki AD, Zafarbakhsh A, Farhadian Dehkordi A PMID: 32595722 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - July 1, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Designing a Recombinant Multi-Epitope Antigen of Echinococcus granulosus to Diagnose Human Cystic Echinococcosis.
Conclusion: Our findings showed high diagnostic accuracy of the ELISA test using the developed recombinant protein, which encourages the use of this recombinant multi-epitope protein for rapid serological diagnosis of hydatidosis. PMID: 32489370 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Design, Construction and Immunogenicity Assessment of pEGFP-N1-KMP11-GP96 (Fusion) as a DNA Vaccine Candidate against Leishmania major Infection in BALB/c Mice.
Conclusion: The constructed fusion could well stimulate both the cellular and humoral immune systems that led to cutaneous lesion healing in mice. PMID: 32489371 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Efficacy and Safety of Zataria multiflora Boiss Essential Oil against Acute Toxoplasmosis in Mice.
Authors: Mahmoudvand H, Tavakoli Kareshk A, Nabi Moradi M, Monzote Fidalgo L, Mirbadie SR, Niazi M, Khatami M Abstract Background: Broad spectrums of pharmacological properties, including antimicrobial activity have been attributed to Zataria multiflora Boiss (Laminaceae). The in vivo efficacy and safety of Z. multiflora essential oil (ZM-EO) were evaluated against acute toxoplasmosis caused by Toxoplasma gondii (Sarcocystidae) in mice. Methods: Z. multiflora (aerial parts) was obtained from the rural districts of Kerman city (Kerman Province) Southwestern Iran, in May of 2016. Male NMRI mice were orally treate...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015-16.
Conclusion: The predominance of C. hominis in the present study may be certifies the importance of anthroponotic transmission of cryptosporidiosis in center of Iran. PMID: 32489373 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Marshallagia marshalli Antigen Strengthens Dendritic Cell Mediated T Lymphocyte Regulation on Asthmatic Patients.
Authors: Hemati F, Mirsadraei M, Hemati M, Mohebalian H, Borji H Abstract Background: The current study was conducted to investigate the antigenic effect of Marshallagia marshalli on the treatment of asthma by measuring the secreted inhibitory cytokine. Methods: Case patients and controls were selected from clinics in Mashhad, Khorasan Razavi Province, Northeastern Iran in 2017-18. In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from 15 patients with asthma and 10 healthy controls and were cultured. PBMCs were then converted to tolerogenic DCs through exposure to GM-CSF, IL-...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The rK39 Antigen from an Iranian Strain of Leishmania infantum: Detection of Anti-Leishmania Antibodies in Humans and Dogs.
This study aimed to prepare and evaluate a new rk39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of VL in humans and dogs. Methods: rK39-based enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for the detection of anti L. infantum antibodies. We screened 84 human sera and 87 dog sera from clinical cases in the endemic area of Meshkin-Shahr, Iran along with 176 sera from healthy controls (collected from 86 humans and 90 dogs) during 2013-2016. Results: Using the rK39 ELISA, a sensitivity of 85.7% (95% CI, 95-99%) and a specificity of 86...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Morphological Description, Phylogenetic and Molecular Analysis of Dirofilaria immitis Isolated from Dogs in the Northwest of Iran.
Conclusion: The SEM technique is an excellent tool for differential recognition of the parasite surface morphology and molecular techniques could differentiate and identify Dirofilaria spp. PMID: 32489376 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

In Vitro Effects of Streptomyces tyrosinase on the Egg and Adult Worm of Toxocara vitulorum.
Conclusion: The current study could offer a promising bio-control agent, Streptomyces tyrosinase, against T. vitulorum alternative to the more expensive synthetic anthelmintics. PMID: 32489377 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

In Vitro Effects of Pumpkin (Cucurbita moschata) Seed Extracts on Echinococcus granulosus Protoscoleces.
Conclusion: Since highest mortality was 16%, the extract did not reach to LD50 (50% mortality). Therefore, the potency of the total extract is not sufficient as potential scolicidal drug. PMID: 32489378 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Clinical Features, Diagnosis and Management of Patients with Suspicion of Fascioliasis in Kohgiluyeh and Boyer-Ahmad Province, Southwestern Iran.
Conclusion: Our observation further confirmed Yasuj district as a human endemic area for fascioliasis in Iran. The study also highlighted the importance of clinical features together with eosinophilia, as key parameters, in the diagnosis of human fascioliasis. Clinicians need to be aware of this disease and should keep in mind fascioliasis when hypereosinophilia present in patients in such endemic areas. PMID: 32489379 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Prevalence of Intestinal Parasites among Rural Inhabitants of Fouman, Guilan Province, Northern Iran with Emphasis on Strongyloides stercoralis.
Authors: Sharifdini M, Ghanbarzadeh L, Barikani A, Saraei M Abstract Background: Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran. Methods: Overall, 31 villages were randomly selected during 2015-2016. Stool samples were collected from 1500 inhabitants aged 2-87. The samples were examined by direct wet smear, formalin ethyl-acetate concentration and agar plate culture. Trichrome staining and modified acid-fast stain...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Experimentally Induced Cerebral Cystic Echinococcosis in Rats: A Suitable Animal Model for Cerebral Echinococcosis.
Conclusion: To the best of our knowledge, this research is the first experimental cerebral hydatidosis arisen from larval stage of Echinococcus granulosus in the animal model. PMID: 32489381 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Herbivores Coprolites from Chehrabad Salt Mine of Zanjan, Iran (Sassanid Era, 224-651 AD) Reveals Eggs of Strongylidae and Anoplocephalidae Helminths.
Conclusion: The presence of two different nematodes in the equids coprolites provide clues of the burden of helminths infection on working animal at the Sassanid time and demonstrates the appropriate preservation condition of biological remains in the ancient salt mine of Chehrabad as well. PMID: 32489382 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Trypanocidal Effect of Nano MOFs-EP on Circulating Forms of Trypanosoma cruzi.
Conclusion: Our results open a new possibility in the biomedical application of MOFs and ergosterol peroxide in the search for new options for the treatment of Chagas disease. PMID: 32489383 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Effects of Water Source, Sanitation and Hygiene on the Prevalence of Schistosoma mansoni among School Age Children in Jawe District, Northwest Ethiopia.
This study aimed to determine the effect of water source, sanitation and hygiene on the prevalence of schistosomiasis among school-age children in Northwest Ethiopia. Methods: A cross-sectional study was conducted from Apr 2016 to Aug 2016. Children were selected by systematic random sampling and Formol Ether Concentration Technique (FECT) was used to identify Schistosoma mansoni infection. Statistical analysis was done using descriptive statistics and strength of association of schistosomiasis with determinant factors was calculated by bivariate analysis. Results: Among 333 children, 7% were infected with Schistos...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococcosis in Baqiyatallah Hospital, Tehran, Iran.
Conclusion: The sequence alignments of the isolates displayed two profiles. All sequenced samples showed E. granulosus sensu stricto (G1) with no organ-related genotype. PMID: 32489385 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Suspected Regional Lymph Node Metastasis in Hepatic Alveolar Echinococcosis: A Case Report.
Authors: Wang Q, Cui Y, Ren L, Wang H, Wang Z, Wang H, Fan H Abstract There is no direct evidence to support the existence of regional lymph node metastatic routes in hepatic alveolar echinococcosis, and only a few literature have been reported. There was a case of hepatic alveolar echinococcosis suspected of metastasis of regional lymph node in Clinical Center for Hydatidosis, Qinghai Province, China. The patient was a 24-yr-old male from pastoral area of Seda County, Sichuan Province, China in 2018. He was admitted to the hospital for physical examination and had no special discomfort. Preoperative examination sh...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The Ruptured Pericardial Cyst to the Right Atrium: A Case Report.
We report a 35-yr-old man referred to Imam Ali Hospital, Kermanshah, western Iran in 2017, with palpitation, chest pain and dyspnea and physical exam revealed sign and symptoms of right atrial compression and tamponade. PMID: 32489387 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Ocular Dirofilariasis after Clinically Manifested Subcutaneous Migration of the Parasite: A Case Report.
Authors: Ondriska F, Boldiš V, Stanislavová M, Antolová D, Miterpáková M, Hanáček A, Vešperová S, Jančovič I Abstract Dirofilaria repens is the causative agent of human subcutaneous or, less often, ocular dirofilariasis. The work presents a rare case of ocular dirofilariasis manifested by previous subcutaneous migration accompanied by severe headache symptoms. In February 2017, a 58-yr-old man from Trnava region, western Slovakia, noticed red and itchy stripes on his left leg. Inflamed but painless stripes disappeared and showed up again every 5-7 days, mig...
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Seroepidemiology of Infection with Toxoplasma gondii in General Population in a Central Mexican City.
Authors: Salas-Pacheco JM, Vértiz-Hernández AA, Sandoval-Carrillo AA, Rangel-López AJ, Antuna-Salcido EI, Salas-Pacheco SM, Sánchez-Anguiano LF, Méndez-Hernández EM, Hernández-Tinoco J, Castellanos-Juárez FX, LA Llave-León O, Alvarado-Esquivel C PMID: 32489389 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Application of Loop-Mediated Isothermal Amplification (LAMP) Assay for Detection of Leishmania infantum Strain from Brazil.
Authors: Silva Nunes Bezerra G, Barbosa Júnior WL, Cintra Leal N, DE Medeiros ZM PMID: 32489390 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - June 4, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The Burden of Cystic Echinococcosis in Kenya: A Review Article.
Conclusion: A better understanding of CE prevalence of different hosts, its transmission pattern and the pathogenicity might make it possible to set up more effective control programs in future. PMID: 32099553 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Comparative Morphologic and Morphometric Study on the Developmental Aspects of In Vitro and In Vivo Reared Echinococcus granulosus Sensu Stricto Using Differential Interference Contrast (DIC)/Nomarski and Phase Contrast Microscopy.
Conclusion: Application of DIC/Nomarski and phase contrast microscopy together with morphometric criteria are useful means for comparing the developmental aspects of in vitro and in vivo reared adults of E. granulosus. PMID: 32099554 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Anti-Leishmanial and Immunomodulatory Effects of Epigallocatechin 3-O-Gallate on Leishmania tropica: Apoptosis and Gene Expression Profiling.
This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against Leishmania tropica stages. Methods: All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. Results: Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable poten...
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Investigation of Giardia intestinalis Genotypes among the Food Handlers of Qazvin, Iran.
Authors: Shahnazi M, Naghizadeh F, Hajialilo E, Alizadeh SA, Saraei M, Alipour M Abstract Background: We aimed to investigate the genotypes of Giardia intestinalis among the food handlers in Qazvin, Iran. Methods: Overall, 1530 stool specimens were collected from the food handlers who visited Shahid Bolandian Health Center, Qazvin, Iran during 2016. Specimens were evaluated by microscopic and concentration methods. Twenty specimens with appropriate number of giardia cysts were selected followed by DNA extraction. Determination of giardia genotypes was achieved through PCR and sequencing the glutamate dehydrogen...
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

A Novel Strategy for Enhance Potentiation of Meglumine antimoniate against Leishmania major In Vitro.
Conclusion: The combination of pore-forming proteins with anti-leishmanial agents could increase the advantage of anti-leishmanial drugs. Since lower concentrations of anti-leishmanial drugs can reduce undesirable side effects of chemotherapy trials carried out in animal models and then in humans with this system. PMID: 32099557 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice.
Conclusion: The successive immunizations with pVAX/TgERK7 can provoke the innate and adaptive immune responses of BALB/c mice, whereas the DNA vaccine-induced immunological efficacy is not sufficient for complete protection the host against T. gondii infection. PMID: 32099558 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

The Prevalence of Intestinal Helminths in Free-Ranging Canids of Mazandaran, Northern Iran.
Conclusion: There is a high prevalence and clear risks of zoonotic helminths in free-ranging carnivores in Mazandaran province, north of Iran. Therefore, understanding the epidemiology of zoonotic parasite infection is useful for health care access both domestic animals and humans health. PMID: 32099559 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Determination of the Infectivity of Cryopreserved Theileria annulata Sporozoites in Tick Derived Stabilates Iran Ak-93 Strain, by In Vivo and In Vitro Methods.
Conclusion: The infectivity of a new prepared tick-derived sporozoite stabilate was confirmed in susceptible calves; by microscopically, post mortem, tick microscopic and molecular assays. Moreover, naïve PBMCs were transformed and proliferated by T. annulata infected tick stabilate to immortal T. annulata schizont infected cell line. The potent infective sporozoite tick derived stabilate could be used for vaccine efficacy and challenge test as well as in vaccine development. PMID: 32099560 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Molecular Identification of Free-Living Amoebae (Naegleria spp., Acanthamoeba spp. and Vermamoeba spp.) Isolated from Un-improved Hot Springs, Guilan Province, Northern Iran.
Conclusion: Although FLA-mediated illnesses are not as high as in environmental distribution, but because of a poor prognosis, more investigations about FLA distribution in hot springs is critical. Hot spring may enhance exposure of the amoebae in individuals. Hence, more attention to unimproved hot springs is needed to prevent free-living amoebae mediated diseases. PMID: 32099561 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

A Metabolomic Investigation of the Effect of Eosin B on Game-tocyte of Plasmodium falciparum Using 1HNMR Spectroscopy.
Conclusion: Eosin B exhibited substantial gametocytocidal activity and affected important drug targets in the Plasmodium. PMID: 32099562 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research

Accumulation of CD4 and CD8 T Cells in Placenta of Malaria Infected Mice Induces the Expression of Hypoxia Inducible Factor-1 α (HIF-1α) and Low Birth Weight (LBW) of the Fetus.
Conclusion: In placental malaria, the expression of CD4 and CD8 induce placental hypoxia characterized by increased expression of HIF-1α that causes LBW. PMID: 32099563 [PubMed] (Source: Iranian Journal of Parasitology)
Source: Iranian Journal of Parasitology - February 28, 2020 Category: Parasitology Tags: Iran J Parasitol Source Type: research