Upconversion nanoparticle-mOrange protein FRET nanoprobes for self-ratiometric/ratiometric determination of intracellular pH, and single cell pH imaging
Publication date: Available online 26 February 2020Source: Biosensors and BioelectronicsAuthor(s): Sandip Ghosh, Yu-Fen Chang, Yang De Ming, Surojit Chattopadhyay (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 27, 2020 Category: Biotechnology Source Type: research

Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs
Publication date: Available online 27 February 2020Source: Biosensors and BioelectronicsAuthor(s): Narendra Kumar, Wenjian Wang, Juan C. Ortiz-Marquez, Matthew Catalano, Mason Gray, Nadia Biglari, Kitadai Hikari, Xi Ling, Jianmin Gao, Tim van Opijnen, Kenneth S. Burch (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 27, 2020 Category: Biotechnology Source Type: research

Evaluation of affinity sensor response kinetics towards dimeric ligands linked with spacers of different rigidity: Immobilized recombinant granulocyte colony-stimulating factor based synthetic receptor binding with genetically engineered dimeric analyte derivatives
Publication date: Available online 25 February 2020Source: Biosensors and BioelectronicsAuthor(s): Ieva Plikusiene, Zigmas Balevicius, Almira Ramanaviciene, Julian Talbot, Gitana Mickiene, Saulius Balevicius, Arunas Stirke, Alla Tereshchenko, Linas Tamosaitis, Gintautas Zvirblis, Arunas Ramanavicius (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 26, 2020 Category: Biotechnology Source Type: research

Fast and efficient deposition of broad range of analytes on substrates for surface enhanced Raman spectroscopy
Publication date: Available online 24 February 2020Source: Biosensors and BioelectronicsAuthor(s): Łukasz Richter, Paweł Albrycht, Monika Księżopolska-Gocalska, Ewa Poboży, Robert Bachliński, Volodymyr Sashuk, Jan Paczesny, Robert Hołyst (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 26, 2020 Category: Biotechnology Source Type: research

Application of a thermophoretic immunoassay in the diagnosis of lupus using outer membrane particles from E. coli
In this study, the outer membrane from E. coli was isolated and used to produce OM particles with a diameter of approximately 100 nm. These prepared OM particles were applied in a thermophoretic immunoassay. First, outer membrane (OM) particles with lipopolysaccharides (LPS) and anti-LPS antibodies were used as a model to demonstrate proof of concept and the difference in E. coli thermophoresis was explained by the changes in the molecular surface area (A) and effective charge (σeff). The hydrodynamic size of the molecules was measured as a changing parameter, molecular surface area (A), by dynamic laser scattering...
Source: Biosensors and Bioelectronics - February 23, 2020 Category: Biotechnology Source Type: research

Niobium-doped TiS2: Formation of TiS3 nanobelts and their effects in enzymatic biosensors
Publication date: Available online 22 February 2020Source: Biosensors and BioelectronicsAuthor(s): Nasuha Rohaizad, Carmen C. Mayorga-Martinez, Zdenek Sofer, Richard D. Webster, Martin PumeraAbstractThere is an assortment of layered transition metal dichalcogenides (TMDs), about 40 reported compounds, each with its unique polymorph and properties. Group 4 TMD, titanium disulfide (TiS2), possess high electronic conductivity and light weight amongst other attractive features. In consideration for electrochemical and thermoelectrical applications, doping is a promising approach to enhance its practicability. The introduction ...
Source: Biosensors and Bioelectronics - February 23, 2020 Category: Biotechnology Source Type: research

Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
Publication date: Available online 21 February 2020Source: Biosensors and BioelectronicsAuthor(s): Jiri Kudr, Lei Zhao, Emily P. Nguyen, Henri Arola, Tarja K. Nevanen, Vojtech Adam, Ondrej Zitka, Arben MerkoçiAbstractThe design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent e...
Source: Biosensors and Bioelectronics - February 23, 2020 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 15 April 2020Source: Biosensors and Bioelectronics, Volume 154Author(s): (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 23, 2020 Category: Biotechnology Source Type: research

Smartphone-assisted robust enzymes@MOFs-based paper biosensor for point-of-care detection
Publication date: Available online 20 February 2020Source: Biosensors and BioelectronicsAuthor(s): Xiaoxue Kou, Linjing Tong, Yujian Shen, Wangshu Zhu, Li Yin, Siming Huang, Fang Zhu, Guosheng Chen, Gangfeng OuyangAbstract:Portable devices featured with fast analysis and affordable methodologies for clinical diagnostics have stimulated the rapid development of point-of-care (POC) technologies, potentially lowering the mortality rate. Herein, we demonstrated a portable, robust, and user-friendly intelligent metal-organic frameworks (MOFs) paper device, called smartphone-assisted biomimetic MOFs nanoreactor colorimetric pape...
Source: Biosensors and Bioelectronics - February 22, 2020 Category: Biotechnology Source Type: research

Capillary-assisted microfluidic biosensing platform captures single cell secretion dynamics in nanoliter compartments
Publication date: Available online 19 February 2020Source: Biosensors and BioelectronicsAuthor(s): Amin Hassanzadeh-Barforoushi, Majid Ebrahimi Warkiani, David Gallego-Ortega, Guozhen Liu, Tracie BarberAbstractCancer cells continuously secrete inflammatory biomolecules which play significant roles in disease progression and tumor metastasis toward secondary sites. Despite recent efforts to capture cancer cells' intercellular secretion heterogeneity using microfluidics, the challenges in operation of these systems as well as the complexity of designing a biosensing assay for long-term and real-time measurement of single cel...
Source: Biosensors and Bioelectronics - February 20, 2020 Category: Biotechnology Source Type: research

Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use
Publication date: Available online 20 February 2020Source: Biosensors and BioelectronicsAuthor(s): Lung-Chieh Chen, Erick Wang, Chun-San Tai, Yuan-Chen Chiu, Chang-Wei Li, Yan-Ren Lin, Tsung-Han Lee, Ching-Wen Huang, Jung-Chih Chen, Wen Liang ChenAbstractElectrochemical biosensors possess numerous desirable qualities for target detection, such as portability and ease of use, and are often considered for point-of-care (POC) development. Label-free affinity electrochemical biosensors constructed with semiconductor manufacturing technology (SMT)-produced electrodes and a streptavidin biomediator currently display the highest ...
Source: Biosensors and Bioelectronics - February 20, 2020 Category: Biotechnology Source Type: research

Self-partitioning SlipChip for slip-induced droplet formation and human papillomavirus viral load quantification with digital LAMP
Publication date: Available online 18 February 2020Source: Biosensors and BioelectronicsAuthor(s): Ziqing Yu, Weiyuan Lyu, Mengchao Yu, Qian Wang, Haijun Qu, Rustem F. Ismagilov, Xu Han, Dongmei Lai, Feng ShenAbstractHuman papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide, and persistent HPV infection can cause warts and even cancer. Nucleic acid analysis of HPV viral DNA can be very informative for the diagnosis and monitoring of HPV. Digital nucleic acid analysis, such as digital PCR and digital isothermal amplification, can provide sensitive detection and precise quantification of ...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Surpassing the detection limit and accuracy of the electrochemical DNA sensor through the application of CRISPR Cas systems
Publication date: Available online 18 February 2020Source: Biosensors and BioelectronicsAuthor(s): Wei Xu, Tian Jin, Yifan Dai, Chung Chiun LiuAbstractRobust developments of personalized medicine for next-generation healthcare highlight the need for sensitive and accurate point-of-care platforms for quantification of disease biomarkers. Broad presentations of clustered regularly interspaced short palindromic repeats (CRISPR) as an accurate gene editing tool also indicate that the high-specificity and programmability of CRISPR system can be utilized as a powerful biosensing tool. Herein, we present a CRISPR Cas system enhan...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Neuroadhesive protein coating improves the chronic performance of neuroelectronics in mouse brain
Publication date: Available online 18 February 2020Source: Biosensors and BioelectronicsAuthor(s): Asiyeh Golabchi, Kevin M. Woeppel, Xia Li, Carl F. Lagenaur, X. Tracy CuiAbstractIntracortical microelectrodes are being developed to both record and stimulate neurons to understand brain circuitry or restore lost functions. However, the success of these probes is hampered partly due to the inflammatory host tissue responses to implants. To minimize the foreign body reactions, L1, a brain derived neuronal specific cell adhesion molecule, has been covalently bound to the neural electrode array surface. Here we evaluated the ch...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Standalone operation of an EGOFET for ultra-sensitive detection of HIV
Publication date: Available online 19 February 2020Source: Biosensors and BioelectronicsAuthor(s): Sunil Kumar Sailapu, Eleonora Macchia, Irene Merino-Jimenez, Juan Pablo Esquivel, Lucia Sarcina, G. Scamarcio, Shelley D. Minteer, Luisa Torsi, Neus SabatéAbstractA point-of-care (POC) device to enable de-centralized diagnostics can effectively reduce the time to treatment, especially in case of infectious diseases. However, many of the POC solutions presented so far do not comply with the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment free, and deliverable to users) guidelines tha...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Gd3+-nanoparticle-enhanced multivalent biosensing that combines magnetic relaxation switching and magnetic separation
Publication date: Available online 19 February 2020Source: Biosensors and BioelectronicsAuthor(s): Yunlei Xianyu, Yongzhen Dong, Zhuo Zhang, Zhanhui Wang, Wenbo Yu, Zhilong Wang, Yiping ChenAbstractIn this work, a multivalent magnetic biosensing strategy has been developed by integrating magnetic separation and magnetic relaxation switching (MRS) where Gd3+-loaded magnetic nanoparticles have been used. As a transition metal ion, Gd3+has multiple unpaired electrons in the d-orbitals that can induce a strong fluctuating magnetic field and thus can reduce the transverse relaxation time (T2), contributing to an enhanced magnet...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Gold-microrods/Pd-nanoparticles/polyaniline-nanocomposite-interface as a peroxidase-mimic for sensitive detection of tropomyosin
In this study, Gold-microrods (AuMRs), Pd-nanoparticles (PdNPs), and Polyaniline (PANI) nanocomposite-interface was fabricated on the screen-printed carbon-microelectrode (SPE). Each layer of the interface was characterised using field emission-scanning electron microscopy (FE-SEM) and cyclic voltammetry (CV). The fabricated SPE/AuMRs/PdNPs/PANI interface demonstrated the highest electronic current and showed excellent peroxidase-mimic towards H2O2 using chronoamperometry (CA). Furthermore, the SPE/AuMRs/PdNPs/PANI interface was utilised for the construction of a highly sensitive label-free electrochemical biosensor for th...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Flexible and self-healing electrochemical hydrogel sensor with high efficiency toward glucose monitoring
Publication date: Available online 19 February 2020Source: Biosensors and BioelectronicsAuthor(s): Zhen Liang, Jieyu Zhang, Can Wu, Xuefeng Hu, Yuhui Lu, Guangfeng Wang, Fei Yu, Xiaojun Zhang, Yunbing WangAbstractThe commercial sensor of a continuous glucose monitoring system suffers from restricted penetration of glucose in the dense sensing coating, uncontrolled leakage of the glucose oxidase and electrocatalytic medium, and susceptibility to mechanical damage. Herein, a self-healing hydrogel based on quaternized chitosan and oxidized dextran was designed, and CeO2/MnO2 hollow nanospheres were covalently linked in the hy...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Switch-conversional ratiometric fluorescence biosensor for miRNA detection
Publication date: Available online 17 February 2020Source: Biosensors and BioelectronicsAuthor(s): Xian Chen, Ke Xu, Jing Li, Ming Yang, Xing Li, Qin Chen, Chunhua Lu, Huanghao YangAbstractMicroRNAs play a crucial role in regulating gene expression and cellular function. Reliable detection of miRNA is highly demanded in clinical diagnosis and therapy. Herein, we designed a structure-convertible DNA switch and constructed a novel switch-conversional ratiometric fluorescence biosensor (SCRF biosensor) for highly sensitive miRNA detection by the use of amplicon fragments to convert the structure of the switch. The DNA switch ...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Sandwich magnetically imprinted immunosensor for electrochemiluminescence ultrasensing diethylstilbestrol based on enhanced luminescence of Ru@SiO2 by CdTe@ZnS quantum dots
Publication date: Available online 17 February 2020Source: Biosensors and BioelectronicsAuthor(s): Wen-Rui Zhao, Yao-Hua Xu, Tian-Fang Kang, Xiao Zhang, Hao Liu, An-Jie Ming, Shui-Yuan Cheng, Feng WeiAbstractA molecularly imprinted magnetic sensor with electroluminescent tags (MIP-ECL sensor) was developed for ultrasensing diethylstilbestrol (DES). A strategy is exploited to enhance ECL emission of the [Ru(bpy)3]2 +-tripropyl amine (TPrA) system by CdTe@ZnS quantum-dots (QDs) through energy transfer. Magnetically molecularly imprinted nanoparticles (MMIPs NPs) based on Fe3O4@SiO2 carriers are artificial, easily reproducibl...
Source: Biosensors and Bioelectronics - February 19, 2020 Category: Biotechnology Source Type: research

Nano-biosensor for the in vitro lactate detection using bi-functionalized conducting polymer/N, S-doped carbon; the effect of αCHC inhibitor on lactate level in cancer cell lines
Publication date: Available online 16 February 2020Source: Biosensors and BioelectronicsAuthor(s): Khalil K. Hussain, N.G. Gurudatt, Mahmood H. Akthar, Kyeong-Deok Seo, Deog-Su Park, Yoon-Bo ShimAbstractA robust amperometric sensor was developed for the lactate detection in the extracellular matrix of cancer cells. The sensor was fabricated by separately immobilizing nicotinamide adenine dinucleotide (NAD+) onto a carboxylic acid group and lactate dehydrogenase (LDH) onto an amine group of bi-functionalized conducting polymer (poly 3-(((2,2':5′,2″-terthiophen)-3′-yl)-5-aminobenzoic acid (pTTABA)) composit...
Source: Biosensors and Bioelectronics - February 17, 2020 Category: Biotechnology Source Type: research

A challenge in biosensors: Is it better to measure a photon or an electron for ultrasensitive detection?
Publication date: Available online 15 February 2020Source: Biosensors and BioelectronicsAuthor(s): Aldo Roda, Fabiana Arduini, Mara Mirasoli, Martina Zangheri, Laura Fabiani, Noemi Colozza, Elisa Marchegiani, Patrizia Simoni, Danila MosconeAbstractBiosensor development exploiting various transduction principles is characterized by a strong competition to reach high detectability, portability and robustness. Nevertheless, a literature-based comparison is not possible, as different conditions are employed in each paper.Herein, we aim at evaluating which measurement, photons or electrons, yields better biosensor performance. ...
Source: Biosensors and Bioelectronics - February 16, 2020 Category: Biotechnology Source Type: research

Differential diagnosis of PRV-infected versus vaccinated pigs using a novel EuNPs-virus antigen probe-based blocking fluorescent lateral flow immunoassay
Publication date: Available online 15 February 2020Source: Biosensors and BioelectronicsAuthor(s): Hui Chen, Xiaoli Zhang, Zhiyuan Jin, Liping Huang, Hanbing Dan, Wei Xiao, Jiajie Liang, Siyi Zou, Yong TangAbstractA novel time-resolved fluorescence blocking lateral flow immunoassay (TRF-BLFIA) was developed for on-site differential diagnosis of pseudorabies virus (PRV)-infected and vaccinated pigs using europium nanoparticles (EuNPs)-labeled virion antigens and high titer PRV gE monoclonal antibodies (PRV gE-mAb). Upon application of a positive serum sample, the specific epitopes of gE protein on the EuNPs-PRV probe were b...
Source: Biosensors and Bioelectronics - February 16, 2020 Category: Biotechnology Source Type: research

Development of microfluidic platform capable of high-throughput absolute quantification of single-cell multiple intracellular proteins from tumor cell lines and patient tumor samples
In conclusion, the developed microfluidic platform was capable of quantifying multiple intracellular proteins from large populations of single cells, and the collected data of protein expressions enabled effective cell-type classifications. (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 14, 2020 Category: Biotechnology Source Type: research

Restriction of intramolecular motions (RIM) by metal-organic frameworks for electrochemiluminescence enhancement:2D Zr12-adb nanoplate as a novel ECL tag for the construction of biosensing platform
Publication date: Available online 13 February 2020Source: Biosensors and BioelectronicsAuthor(s): Li-Ying Yao, Fang Yang, Gui-Bing Hu, Yang Yang, Wei Huang, Wen-Bin Liang, Ruo Yuan, Dong-Rong XiaoAbstractHerein, a new phenomenon of enhanced electrochemiluminescence (ECL) emission by restricting intramolecular motion in the 2D ultra-thin Zr12-adb (adb = 9,10-anthracene dibenzoate) metal-organic framework (MOF) nanoplate was discovered for the first time. The coordination immobilization of adb in porous ultra-thin Zr12-adb nanoplate endowed the Zr12-adb excellent ECL performance, including stronger ECL signal and higher...
Source: Biosensors and Bioelectronics - February 14, 2020 Category: Biotechnology Source Type: research

Biomimetic electrochemical sensor for the highly selective detection of azithromycin in biological samples
Publication date: Available online 13 February 2020Source: Biosensors and BioelectronicsAuthor(s): Ioan-Adrian Stoian, Bogdan-Cezar Iacob, Cosmina-Larisa Dudaș, Lucian Barbu-Tudoran, Diana Bogdan, Iuliu Ovidiu Marian, Ede Bodoki, Radu OpreanAbstractA highly selective and sensitive molecularly imprinted polymer (MIP)-based electrochemical sensor was fabricated for the determination of azithromycin, a broad-spectrum macrolide antibiotic, from various biological samples (urine, tears, plasma). The reversible boronate ester bond-mediated, thin (∼75 nm) MIP-based biomimetic recognition layer was electrodeposited in non-a...
Source: Biosensors and Bioelectronics - February 14, 2020 Category: Biotechnology Source Type: research

Tuning the specificity of DNA probes using bulge-loops for low-abundance SNV detection
Publication date: Available online 12 February 2020Source: Biosensors and BioelectronicsAuthor(s): Shulian Bai, Bangtian Xu, Yangli Zhang, Yuhong Zhang, Hao Dang, Shuangshuang Yang, Chen Zuo, Li Zhang, Junjie Li, Guoming XieAbstractTuning the free energy difference between a molecular probe and the target has been regarded as a feasible way to realize selective mutant recognition. But due to limited extent of variation on the probing sequences, it remains a challenge to moderately leverage the thermodynamic kinetics simply by changing the base composition of probes. Herein we propose the modulation of discrimination capabi...
Source: Biosensors and Bioelectronics - February 14, 2020 Category: Biotechnology Source Type: research

Retraction notice to “Detection of p53 gene point mutation using sequence-specific molecularly imprinted PoPD electrode” [Biosens. Bioelectron. 35 (1) (2012) 224-229]
Publication date: 15 April 2020Source: Biosensors and Bioelectronics, Volume 154Author(s): Ashutosh Tiwari, Swapneel R. Deshpande, Hisatoshi Kobayashi, Anthony P.F. Turner (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 14, 2020 Category: Biotechnology Source Type: research

A signal on-off ratiometric electrochemical sensor coupled with a molecular imprinted polymer for selective and stable determination of imidacloprid
Publication date: Available online 11 February 2020Source: Biosensors and BioelectronicsAuthor(s): Wen Zhang, Chao Liu, Kuiguo Han, Xiaoou Wei, Yiwei Xu, Xiaobo Zou, Han Zhang, Zhiyu ChenAbstractThis work presents a signal on-off ratiometric electrochemical sensor coupled with a molecular imprinted polymer (MIP) for imidacloprid (IMI) determination. The ratiometric strategy corrects the detection results by setting 6-(Ferrocenyl)hexanethiol (FcHT) as an internal reference. The MIP membrane, as a molecular recognition receptor, has a three-dimensional structure and is complementary in the shape and chemical functionality to...
Source: Biosensors and Bioelectronics - February 13, 2020 Category: Biotechnology Source Type: research

Sensitive detection of influenza a virus based on a CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test
In this study, we developed a CdSe/CdS/ZnS quantum dot (QD) fluorescent dye for rapid and sensitive detection of two common subtypes (H1N1 and H3N2) of influenza A virus, and examined its utility. CdSe/CdS/ZnS QD was conjugated with antibody (Ab) after conjugation with latex, making QD conjugate of QD + Latex + Ab. A stable photoluminescence of QD conjugate and advantage of CdSe/CdS/ZnS QD used was characterized in this study. The performance of a rapid fluorescent immunochromatographic test (FICT) employing QD conjugate (QD-FICT) in detecting influenza A/H1N1 was 8-fold and 64-fold higher than that of ...
Source: Biosensors and Bioelectronics - February 13, 2020 Category: Biotechnology Source Type: research

Iron nanoflorets on 3D-graphene-nickel: A ‘Dandelion’ nanostructure for selective deoxynivalenol detection
This study is focused on developing a selective biosensing system using iron nanoflorets graphene nickel (INFGN) as the transducer and a specific aptamer as the biorecognition element. 3D-graphene is incorporated using a low-pressure chemical vapour deposition followed by the decoration of iron nanoflorets using electrochemical deposition. INFGN enables a feasible bio-capturing due to its large surface area. The X-ray photoelectron spectroscopy analysis confirms the presence of the hydroxyl groups on the INFGN surface, which acts as the linker. Clear Fourier-transform infrared peak shifts affirm the changes with surface ch...
Source: Biosensors and Bioelectronics - February 11, 2020 Category: Biotechnology Source Type: research

A self-powered photoelectrochemical cathodic aptasensor for the detection of 17β-estradiol based on FeOOH/In2S3 photoanode
Publication date: Available online 11 February 2020Source: Biosensors and BioelectronicsAuthor(s): Yuewen Li, Lei Liu, Jinhui Feng, Xiang Ren, Yong Zhang, Tao Yan, Xuejing Liu, Qin WeiAbstractIn this work, a novel self-powered photoelectrochemical (PEC) aptasensor integrated photoanode and photocathode for the accurate and selective detection of 17β-estradiol (E2) was proposed for the first time. FeOOH/In2S3 heterojunction was built initially and used as a substitute for platinum (Pt) counter electrode. The matched band gap edge of FeOOH and In2S3 facilitated the transfer of photo-generate electrons to photoanode, whi...
Source: Biosensors and Bioelectronics - February 11, 2020 Category: Biotechnology Source Type: research

Recent advances in single extracellular vesicle detection methods
Publication date: Available online 9 February 2020Source: Biosensors and BioelectronicsAuthor(s): Su Wang, Adeel Khan, Rongrong Huang, Shiyi Ye, Kaili Di, Tao Xiong, Zhiyang LiAbstractExtracellular vesicles (EVs) are secreted by a variety of cells. They are known for their pertinent role in intercellular communication, and participation in different pathological processes, making them ideal candidate for utilization as a biomarker for diagnosis and treatment of diseases. In contemporary years, the concept of a well-established liquid biopsy technology, and detection and utilization of EVs as a biomarkers have received unpr...
Source: Biosensors and Bioelectronics - February 10, 2020 Category: Biotechnology Source Type: research

Carbon dots: Current advances in pathogenic bacteria monitoring and prospect applications
Publication date: Available online 8 February 2020Source: Biosensors and BioelectronicsAuthor(s): Fangchao Cui, Yongli Ye, Jianfeng Ping, Xiulan SunAbstract:Pathogenic bacterial infections are a significant threat to human safety and health. Recent researches on the application of nanoparticles as imaging, detecting agents have evidenced their huge potential for infectious disease management. Among these nanoparticles, carbon dots (CDs) have attracted much attention as a new and innovative nanoparticle owing to their unique optical and physicochemical properties as well as their higher biosafety. Thus, CDs are becoming sup...
Source: Biosensors and Bioelectronics - February 10, 2020 Category: Biotechnology Source Type: research

An internet of things-based intensity and time-resolved fluorescence reader for point-of-care testing
Publication date: Available online 7 February 2020Source: Biosensors and BioelectronicsAuthor(s): O. Alonso, N. Franch, J. Canals, K. Arias-Alpízar, E. de la Serna, E. Baldrich, A. DiéguezAbstractA miniature internet of things (IoT)-based point-of-care testing (PoCT) fluorescence reader, able to perform both intensity and time-resolved measurements of different fluorescent tags, is presented. This low cost platform has been conceived for performing tests in low-resource and remote settings, displaying versatile performance and yet simple operation. It consists on an external case of 43 × 30 ×...
Source: Biosensors and Bioelectronics - February 8, 2020 Category: Biotechnology Source Type: research

Isolation and analysis of extracellular vesicles in a Morpho butterfly wing-integrated microvortex biochip
Publication date: Available online 7 February 2020Source: Biosensors and BioelectronicsAuthor(s): Shanying Han, Yueshuang Xu, Jie Sun, Yufeng Liu, Yuanjin Zhao, Weiguo Tao, Renjie ChaiAbstractWith the function of mediating intercellular communication between cells, extracellular vesicles (EVs) have been intently studied for their physiopathology and clinical application values. However, efficient EV isolation from biological fluids remains a significant challenge. To address this, this work constructs a new microvortex chip that can isolate EVs efficiently by integrating the lipid nanoprobe modified Morpho Menelaus (M. Men...
Source: Biosensors and Bioelectronics - February 8, 2020 Category: Biotechnology Source Type: research

Enhanced electrochemical oxidation of ethanol using a hybrid catalyst cascade architecture containing pyrene-TEMPO, oxalate decarboxylase and carboxylated multi-walled carbon nanotube
Publication date: Available online 7 February 2020Source: Biosensors and BioelectronicsAuthor(s): Jefferson Honorio Franco, Kevin J. Klunder, Jack Lee, Victoria Russell, Adalgisa R. de Andrade, Shelley D. MinteerAbstractThe work presented herein demonstrates a hybrid bi-catalytic architecture for the complete electrochemical oxidation of ethanol. The new catalytic system contains pyrene-TEMPO (TEMPO = 2,2,6,6-tetramethylpiperidinyl-N-oxyl) immobilized on the surface of carboxylated multi-walled carbon nanotubes (MWCNT-COOH), and oxalate decarboxylase enzyme (OxDc) immobilized onto a carbon cloth electrode. Electrolysis...
Source: Biosensors and Bioelectronics - February 8, 2020 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 1 April 2020Source: Biosensors and Bioelectronics, Volume 153Author(s): (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - February 7, 2020 Category: Biotechnology Source Type: research

Femtomolar sensing of Alzheimer's tau proteins by water oxidation-coupled photoelectrochemical platform
Publication date: Available online 5 February 2020Source: Biosensors and BioelectronicsAuthor(s): Kayoung Kim, Chan Beum ParkAbstractAlzheimer's disease (AD) is the most prevalent neurodegenerative disorder. A key pathogenic event of AD is the formation of intracellular neurofibrillary tangles that are mainly composed of tau proteins. Here, we report on ultrasensitive detection of total tau (t-tau) proteins using an artificial electron donor-free, BiVO4-based photoelectrochemical (PEC) analysis. The platform was constructed by incorporating molybdenum (Mo) dopant and iron oxyhydroxide (FeOOH) ad-layer into the BiVO4 photoe...
Source: Biosensors and Bioelectronics - February 7, 2020 Category: Biotechnology Source Type: research

Highly fluorescent copper nanoclusters for sensing and bioimaging
Publication date: Available online 6 February 2020Source: Biosensors and BioelectronicsAuthor(s): Yu An, Ying Ren, Michael Bick, Aleksandra Dudek, Ethan Hong-Wang Waworuntu, Jing Tang, Jun Chen, Baisong ChangAbstractMetal nanoclusters (NCs), typically consisting of a few to tens of metal atoms, bridge the gap between organometallic compounds and crystalline metal nanoparticles. As their size approaches the Fermi wavelength of electrons, metal NCs exhibit discrete energy levels, which in turn result in the emergence of intriguing physical and chemical (or physicochemical) properties, especially strong fluorescence. Compared...
Source: Biosensors and Bioelectronics - February 7, 2020 Category: Biotechnology Source Type: research

Chemically modified carbon nitride-chitin-acetic acid hybrid as a metal-free bifunctional nanozyme cascade of glucose oxidase-peroxidase for “click off” colorimetric detection of peroxide and glucose
Publication date: Available online 4 February 2020Source: Biosensors and BioelectronicsAuthor(s): Pavel Sengupta, Krishnendu Pramanik, Pallab Datta, Priyabrata SarkarAbstractHybrid nanomaterials-based artificial enzymes with numerous utilities are necessary to develop future bionic devices in mimicking physiological processes. This paper demonstrates bifunctional enzyme mimicking roles of a metal-free nanozyme hybrid of chemically modified graphitic carbon nitride (MGCN), chitin and acetic acid (AcOH). The MGCN exhibited glucose oxidase-mimicking activity and chitin-AcOH mirrored peroxidase. MGCN-chitin-AcOH when in contac...
Source: Biosensors and Bioelectronics - February 5, 2020 Category: Biotechnology Source Type: research

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system
Publication date: Available online 4 February 2020Source: Biosensors and BioelectronicsAuthor(s): Qian He, Dongmei Yu, Mengdi Bao, Grant Korensky, Juhong Chen, Mingyeong Shin, Juwon Kim, Myeongkee Park, Peiwu Qin, Ke DuAbstractHere we report the development of a high throughput, all-solution phase, and isothermal detection system for African Swine Fever Virus (ASFV). CRISPR-Cas12a programmed with a CRISPR RNA (crRNA) is used to detect ASFV target DNA. Upon ASFV DNA binding, the Cas12a/crRNA/ASFV DNA complex becomes activated and degrades a fluorescent single stranded DNA (ssDNA) reporter present in the assay. We combine th...
Source: Biosensors and Bioelectronics - February 5, 2020 Category: Biotechnology Source Type: research

Review: Electrochemical DNA sensing – Principles, commercial systems, and applications
Publication date: Available online 3 February 2020Source: Biosensors and BioelectronicsAuthor(s): Martin Trotter, Nadine Borst, Roland Thewes, Felix von StettenAbstractDriven by the vision of robust and portable, yet sensitive DNA detection systems for point-of-need applications, the development of electrochemical DNA sensing principles has been of high interest. Many different principles have been developed and these are regularly reviewed. However, the maturity of electrochemical principles and their ability to produce competitive real-world applications is rarely assessed.In this review, general electrochemical DNA sens...
Source: Biosensors and Bioelectronics - February 4, 2020 Category: Biotechnology Source Type: research

Bioelectronic sensor mimicking the human neuroendocrine system for the detection of hypothalamic-pituitary-adrenal axis hormones in human blood
Publication date: Available online 3 February 2020Source: Biosensors and BioelectronicsAuthor(s): Seung hwan Lee, Minju Lee, Heehong Yang, Youngtak Cho, Seunghun Hong, Tai Hyun ParkAbstractIn the neuroendocrine system, corticotropin-releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) play important roles in the regulation of the hypothalamic-pituitary-adrenal (HPA) system. Disorders of the HPA system lead to physiological problems, such as Addison’s disease and Cushing’s syndrome. Therefore, detection of CRH and ACTH is essential for diagnosing disorders related to the HPA system. Herein, receptors o...
Source: Biosensors and Bioelectronics - February 4, 2020 Category: Biotechnology Source Type: research

An integrated model for bead-based immunoassays
Publication date: Available online 3 February 2020Source: Biosensors and BioelectronicsAuthor(s): Dan Wu, Joel VoldmanAbstractBead-based immunoassays have shown great promise for rapid and sensitive protein quantification. However, there still lacks holistic understanding of assay performance that can inform assay design and optimization. In this paper, we present an integrated mathematical model for surface coverage bead-based assays. This model examines the building blocks of surface coverage assays, including heterogeneous binding of analyte molecules on bead or sensor surfaces, attachment of bead labels to sensor surfa...
Source: Biosensors and Bioelectronics - February 4, 2020 Category: Biotechnology Source Type: research

Electrochemical immunosensor functionalized with nanobodies for the detection of the toxic microalgae Alexandrium minutum using glassy carbon electrode modified with gold nanoparticles
Publication date: Available online 3 February 2020Source: Biosensors and BioelectronicsAuthor(s): Sandra Oloketuyi, Elisa Mazzega, Janez Zavašnik, Kingkan Pungjunum, Kurt Kalcher, Ario de Marco, Eda MehmetiAbstractIn this work an electrochemical immunosensor for the toxic microalgae Alexandrium minutum (A. minutum AL9T) detection is described. A glassy carbon electrode (GCE) was modified by depositing gold nanoparticles followed by L-cysteine for obtaining a self-assembled monolayer. The SpyTagged nanobody C1, specific for the A. minutum toxic strain AL9T, was then covalently immobilized via SpyCatcher on the surfac...
Source: Biosensors and Bioelectronics - February 4, 2020 Category: Biotechnology Source Type: research

Rapid and low-cost quantitative detection of creatinine in human urine with a portable Raman spectrometer
Publication date: Available online 31 January 2020Source: Biosensors and BioelectronicsAuthor(s): Wei Zhu, Bao-Ying Wen, Ling-Jun Jie, Xiang-Dong Tian, Zhi-Lin Yang, Petar M. Radjenovic, Shi-Yi Luo, Zhong-Qun Tian, Jian-Feng LiAbstractThe creatinine concentration of human urine is closely related to human kidney health and its rapid, quantitative, and low-cost detection has always been demanded. Herein, a surface-enhanced Raman spectroscopic (SERS) method for rapid and cost-effective quantification of creatinine concentrations in human urine was developed. A Au nanoparticle solution (Au sol) was used as a SERS substrate an...
Source: Biosensors and Bioelectronics - February 1, 2020 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 15 March 2020Source: Biosensors and Bioelectronics, Volume 152Author(s): (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - January 30, 2020 Category: Biotechnology Source Type: research

High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array
Publication date: Available online 28 January 2020Source: Biosensors and BioelectronicsAuthor(s): Yunpeng Fan, Xiaolei Duan, Min Zhao, Xiaotong Wei, Jiangling Wu, Wenqin Chen, Ping Liu, Wei Cheng, Quan Cheng, Shijia DingAbstractNon-small cell lung cancer (NSCLC) have been reported to secret a high concentration of exosomes into blood circulatory system, which is one of sensitive and non-invasive biomarkers for NSCLC's early-stage diagnosis. But it is still lack of feasible and accurate methods to analyze the different NSCLC cells-derived exosomes. Herein, we built a SPRi biosensing assay for high-sensitive and multiplex ch...
Source: Biosensors and Bioelectronics - January 30, 2020 Category: Biotechnology Source Type: research

Sensors design based on hybrid gold-silica nanostructures
Publication date: Available online 28 January 2020Source: Biosensors and BioelectronicsAuthor(s): Elnaz Bagheri, Legha Ansari, Elham Sameiyan, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona AlibolandiAbstractHybrid silica-gold based sensors show attractive performance in sensing technologies. Due to their interesting optical properties and biological compatibility, gold nanoparticles (AuNPs) have been extensively implemented in sensing technology.Hybridization of AuNPs with silica NPs as a material with unique characteristic comprising large surface area, narrow pore distribution, tunable pore size and exc...
Source: Biosensors and Bioelectronics - January 30, 2020 Category: Biotechnology Source Type: research