Combining deep learning and droplet microfluidics for rapid and label-free antimicrobial susceptibility testing of colistin
Biosens Bioelectron. 2024 Apr 16;257:116301. doi: 10.1016/j.bios.2024.116301. Online ahead of print.ABSTRACTEfficient tools for rapid antibiotic susceptibility testing (AST) are crucial for appropriate use of antibiotics, especially colistin, which is now often considered a last resort therapy with extremely drug resistant Gram-negative bacteria. Here, we developed a rapid, easy and miniaturized colistin susceptibility assay based on microfluidics, which allows for culture and high-throughput analysis of bacterial samples. Specifically, a simple microfluidic platform that can easily be operated was designed to encapsulate ...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Justine Riti Guillaume Sutra Thierry Naas Herv é Volland St éphanie Simon Karla Perez-Toralla Source Type: research
A cell-free biosensor for multiplexed and sensitive detection of biological warfare agents
This study introduces a cell-free biosensor for BWA detection by converting the 16S rRNA of targeted pathogens into detectable functional protein molecules. The modular nature of this approach allows for the flexible configuration of pathogen detection, enabling the simultaneous identification of multiple pathogenic 16S rRNAs through customized reporter proteins for each targeted sequence. Furthermore, we demonstrate how this method integrates with techniques utilizing retroreflective Janus particles (RJPs) for facile and highly sensitive pathogen detection. The cell-free biosensor, employing RJPs to measure the reflection...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Yu Jin Park Sunjoo Choi Kyung Won Lee So-Yoon Park Dong-Yeon Song Tae Hyeon Yoo Dong-Myung Kim Source Type: research
NIR-driven multifunctional PEC biosensor based on aptamer-modified PDA/MnO < sub > 2 < /sub > photoelectrode for bacterial detection and inactivation
Biosens Bioelectron. 2024 Apr 20;257:116320. doi: 10.1016/j.bios.2024.116320. Online ahead of print.ABSTRACTSensitive detection and effective inactivation of bacteria are essential in preventing foodborne bacterial infection that poses a significant threat to human health. Herein, a near-infrared (NIR)-driven multifunctional photoelectrochemical (PEC) biosensor was constructed for detection and inactivation of S. aureus. Based on the covalent bonding between amine and carboxyl groups, carboxyl-functionalized SA31 aptamer was immobilized on the PDA/MnO2 photoelectrode. In the presence of S. aureus, SA31 aptamer can specific...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Anni Cui Lihua Dong Yiting Hou Xin Mu Yu Sun Huan Wang Xiahua Zhong Guiye Shan Source Type: research
A dual-channel electrochemical biosensor enables concurrent detection of pathogens and antibiotic resistance
Biosens Bioelectron. 2024 Apr 20;257:116314. doi: 10.1016/j.bios.2024.116314. Online ahead of print.ABSTRACTDiarrheagenic E. coli infections, commonly treated with β-lactam antibiotics, contribute to antibiotic resistance - a pressing public health concern. Rapid monitoring of pathogen antibiotic resistance is vital to combat antimicrobial spread. Current bacterial diagnosis methods identify pathogens or determine antibiotic resistance separately, necessitating multiple assays. There is an urgent need for tools that simultaneously identify infectious agents and their antibiotic resistance at the point of care (POC). We de...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Dharanivasan Gunasekaran Irina Rostovsky David Taussig Talya Bar-Am Yariv Wine Neta Sal-Man Sefi Vernick Source Type: research
Vortexing-generated high throughput single-cell droplets for facile analysis of multiplexed microRNA dynamic secretion
Biosens Bioelectron. 2024 Apr 17;257:116303. doi: 10.1016/j.bios.2024.116303. Online ahead of print.ABSTRACTDiscriminating secretory phenotypes provides a direct, intact, and dynamic way to evaluate the heterogeneity in cell states and activation, which is significant for dissecting non-genetic heterogeneity for human health studies and disease diagnostics. In particular, secreted microRNAs, soluble signaling molecules released by various cells, are increasingly recognized as a critical mediator for cell-cell communication and the circulating biomarkers for disease diagnosis. However, single-cell analysis of secreted miRNA...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Fengjiao Zhu Yangyang Long Weiwei Shi Bin Li Yahui Ji Xue Bai Xianming Liu Dongyuan Qi Bo Sun Fuyin Zhang Tingjiao Liu Bingcheng Lin Yao Lu Source Type: research
Culture-free detection of β-lactamase-Producing bacteria in urinary tract infections using a paper sensor
Biosens Bioelectron. 2024 Apr 22;257:116300. doi: 10.1016/j.bios.2024.116300. Online ahead of print.ABSTRACTDeveloping simple, inexpensive, fast, sensitive, and specific probes for antibiotic-resistant bacteria is crucial for the management of urinary tract infections (UTIs). We here propose a paper-based sensor for the rapid detection of β-lactamase-producing bacteria in the urine samples of UTI patients. By conjugating a strongly electronegative group -N+(CH3)3 with the core structures of cephalosporin and carbapenem antibiotics, two visual probes were achieved to respectively target the extended-spectrum/AmpC β-lactam...
Source: Biosensors and Bioelectronics - April 24, 2024 Category: Biotechnology Authors: Guangming Liu Wenshuai Li Siya Li Jia Xu Xinsheng Wang Hua Xu Dingbin Liu Hongmei Gao Source Type: research
Wearable smart contact lenses: A critical comparison of three physiological signals outputs for health monitoring
Biosens Bioelectron. 2024 Apr 16;257:116284. doi: 10.1016/j.bios.2024.116284. Online ahead of print.ABSTRACTSmart contact lenses (SCLs) have been considered as novel wearable devices for out-of-hospital and self-monitoring applications. They are capable of non-invasively and continuously monitoring physiological signals in the eyes, including vital biophysical (e.g., intraocular of pressure, temperature, and electrophysiological signal) and biochemical signals (e.g., pH, glucose, protein, nitrite, lactic acid, and ions). Recent progress mainly focuses on the rational design of wearable SCLs for physiological signal monitor...
Source: Biosensors and Bioelectronics - April 24, 2024 Category: Biotechnology Authors: Fei Han Juju Li Pingping Xiao Yanshen Yang Hao Liu Zhao Wei Yuan He Feng Xu Source Type: research
Polymeric optical fiber biosensor with PAMAM dendrimer-based surface modification and PlGF detection for pre-eclampsia diagnosis
Biosens Bioelectron. 2024 Apr 18;257:116312. doi: 10.1016/j.bios.2024.116312. Online ahead of print.ABSTRACTPre-eclampsia (PE) is a life-threatening complication that occurs during pregnancy, affecting a large number of pregnant women and newborns worldwide. Rapid, on-site and affordable screening of PE at an early stage is necessary to ensure timely treatment and minimize both maternal and neonatal morbidity and mortality rates. Placental growth factor (PlGF) is an angiogenic blood biomarker used for PE diagnosis. Herein, we report the plasmonic fiber optic absorbance biosensor (P-FAB) strategy for detecting PlGF at femto...
Source: Biosensors and Bioelectronics - April 24, 2024 Category: Biotechnology Authors: Ratan Kumar Chaudhary Narayanan Madaboosi Jitendra Satija Balaji Nandagopal Ramprasad Srinivasan V V R Sai Source Type: research
Functionalized gold nanoparticle enhanced nanorod hyperbolic metamaterial biosensor for highly sensitive detection of carcinoembryonic antigen
In this study, a nanorod HMM (NHMM) biosensor based on refractive index changes for carcinoembryonic antigen (CEA) detection is developed using secondary antibody modified gold nanoparticle (AuNP-Ab2) nanocomposites as signal amplification element for the first time. Numerical analysis based on finite element method is conducted to simulate the perturbation of the electric field of bulk plasmon polariton (BPP) supported by a NHMM in the presence of a AuNP. The simulation reveals an enhancement of the localized electric field, which arises from the resonant coupling of BPP to the localized surface plasmon resonance supporte...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Huimin Wang Jintao Cai Tao Wang Ruoqin Yan Ming Shen Jinyan Zhang Xinzhao Yue Lu Wang Xuyang Yuan Enze Lv Jinwei Zeng Xuewen Shu Jian Wang Source Type: research
Paper-based loop-mediated isothermal amplification and CRISPR integrated platform for on-site nucleic acid testing of pathogens
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates the need for thermal cycling, resulting in simplified instrumentation, and the CRISPR-associated protein (Cas 12a) system eliminates false positive signals from LAMP products, resulting in highly selective and sensitive assays. We optimized the assay to perform both amplification and detection entir...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Anindita Sen Manaswini Masetty Sasanka Weerakoon Calum Morris Jagjit S Yadav Senu Apewokin Jennifer Trannguyen Murray Broom Aashish Priye Source Type: research
Functionalized gold nanoparticle enhanced nanorod hyperbolic metamaterial biosensor for highly sensitive detection of carcinoembryonic antigen
In this study, a nanorod HMM (NHMM) biosensor based on refractive index changes for carcinoembryonic antigen (CEA) detection is developed using secondary antibody modified gold nanoparticle (AuNP-Ab2) nanocomposites as signal amplification element for the first time. Numerical analysis based on finite element method is conducted to simulate the perturbation of the electric field of bulk plasmon polariton (BPP) supported by a NHMM in the presence of a AuNP. The simulation reveals an enhancement of the localized electric field, which arises from the resonant coupling of BPP to the localized surface plasmon resonance supporte...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Huimin Wang Jintao Cai Tao Wang Ruoqin Yan Ming Shen Jinyan Zhang Xinzhao Yue Lu Wang Xuyang Yuan Enze Lv Jinwei Zeng Xuewen Shu Jian Wang Source Type: research
Paper-based loop-mediated isothermal amplification and CRISPR integrated platform for on-site nucleic acid testing of pathogens
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates the need for thermal cycling, resulting in simplified instrumentation, and the CRISPR-associated protein (Cas 12a) system eliminates false positive signals from LAMP products, resulting in highly selective and sensitive assays. We optimized the assay to perform both amplification and detection entir...
Source: Biosensors and Bioelectronics - April 23, 2024 Category: Biotechnology Authors: Anindita Sen Manaswini Masetty Sasanka Weerakoon Calum Morris Jagjit S Yadav Senu Apewokin Jennifer Trannguyen Murray Broom Aashish Priye Source Type: research
Recent advances in implantable sensors and electronics using printable materials for advanced healthcare
Biosens Bioelectron. 2024 Apr 17;257:116302. doi: 10.1016/j.bios.2024.116302. Online ahead of print.ABSTRACTThis review article focuses on the recent printing technological progress in healthcare, underscoring the significant potential of implantable devices across diverse applications. Printing technologies have widespread use in developing health monitoring devices, diagnostic systems, and surgical devices. Recent years have witnessed remarkable progress in fabricating low-profile implantable devices, driven by advancements in printing technologies and nanomaterials. The importance of implantable biosensors and bioelectr...
Source: Biosensors and Bioelectronics - April 22, 2024 Category: Biotechnology Authors: Seunghyeb Ban Haran Lee Jiehao Chen Hee-Seok Kim Yuhang Hu Seong J Cho Woon-Hong Yeo Source Type: research
Rational design of nanozyme with integrated sample pretreatment for colorimetric biosensing
Biosens Bioelectron. 2024 Apr 17;257:116310. doi: 10.1016/j.bios.2024.116310. Online ahead of print.ABSTRACTNanozymes have been widely used in the field of biosensing owing to their high stability, low cost, adjustable catalytic activity, and convenient modification. However, achieving high selectivity and sensitivity simultaneously in nanozyme-based colorimetric sensing remains a major challenge. Nanozymes are nanomaterials with enzyme-simulating activity that are often used as solid-phase adsorbents for sample pretreatment. Our design strategy integrated sample pretreatment function into the nanozyme through separation a...
Source: Biosensors and Bioelectronics - April 21, 2024 Category: Biotechnology Authors: Jia Wu Ling Liang Shuishi Li Yuan Qin Shulin Zhao Fanggui Ye Source Type: research
Highly sensitive humidity sensor based on composite film of partially reduced graphene oxide and bacterial cellulose
In this study, a humidity sensing composite film with high sensitivity and short response time was made by using the mixture of graphene oxide (GO) and bacterial cellulose (BC) with simple dry film-forming method. L-ascorbic acid was used as reducing agent to reduce GO and improve the conductivity of GO/BC composite film (BG). The influence of different BC contents and the different reduction degree on the resistance change rate of composite film was investigated in details. The maximum resistance change rate of partially reduced BG humidity sensitive composite film reached up to 94%, and the response and recovery time wer...
Source: Biosensors and Bioelectronics - April 21, 2024 Category: Biotechnology Authors: Jingyi Song Mingshuai Fan Rui Zhang Meijie Qu Ping Tang Hai Wang Yuezhen Bin Source Type: research
