Hybridized nanolayer modified Ω-shaped fiber-optic synergistically enhances localized surface plasma resonance for ultrasensitive cytosensor and efficient photothermal therapy
Biosens Bioelectron. 2021 Aug 30;194:113599. doi: 10.1016/j.bios.2021.113599. Online ahead of print.ABSTRACTInadequate sensitivity and side-effect are the main challenges to develop cytosensors combining with therapeutic potential simultaneously for cancer diagnosis and treatment. Herein, localized surface plasma resonance (LSPR) based on hybridized nanolayer modified Ω-shaped fiber-optic (HN/Ω-FO) was developed to integrate cytosensor and plasmonic photothermal treatment (PPT). On one hand, hybridized nanolayers improve the coverage of nanoparticles and refractive index sensitivity (RIS). Moreover, the hybridi...
Source: Biosensors and Bioelectronics - September 14, 2021 Category: Biotechnology Authors: Lu He Fan He Yanting Feng Xu Wang Yongxin Li Yonghui Tian Aihua Gao Pei Zhang Xinyuan Qi Zewei Luo Yixiang Duan Source Type: research

Hybridized nanolayer modified Ω-shaped fiber-optic synergistically enhances localized surface plasma resonance for ultrasensitive cytosensor and efficient photothermal therapy
Biosens Bioelectron. 2021 Aug 30;194:113599. doi: 10.1016/j.bios.2021.113599. Online ahead of print.ABSTRACTInadequate sensitivity and side-effect are the main challenges to develop cytosensors combining with therapeutic potential simultaneously for cancer diagnosis and treatment. Herein, localized surface plasma resonance (LSPR) based on hybridized nanolayer modified Ω-shaped fiber-optic (HN/Ω-FO) was developed to integrate cytosensor and plasmonic photothermal treatment (PPT). On one hand, hybridized nanolayers improve the coverage of nanoparticles and refractive index sensitivity (RIS). Moreover, the hybridi...
Source: Biosensors and Bioelectronics - September 14, 2021 Category: Biotechnology Authors: Lu He Fan He Yanting Feng Xu Wang Yongxin Li Yonghui Tian Aihua Gao Pei Zhang Xinyuan Qi Zewei Luo Yixiang Duan Source Type: research

Hybridized nanolayer modified Ω-shaped fiber-optic synergistically enhances localized surface plasma resonance for ultrasensitive cytosensor and efficient photothermal therapy
Biosens Bioelectron. 2021 Aug 30;194:113599. doi: 10.1016/j.bios.2021.113599. Online ahead of print.ABSTRACTInadequate sensitivity and side-effect are the main challenges to develop cytosensors combining with therapeutic potential simultaneously for cancer diagnosis and treatment. Herein, localized surface plasma resonance (LSPR) based on hybridized nanolayer modified Ω-shaped fiber-optic (HN/Ω-FO) was developed to integrate cytosensor and plasmonic photothermal treatment (PPT). On one hand, hybridized nanolayers improve the coverage of nanoparticles and refractive index sensitivity (RIS). Moreover, the hybridi...
Source: Biosensors and Bioelectronics - September 14, 2021 Category: Biotechnology Authors: Lu He Fan He Yanting Feng Xu Wang Yongxin Li Yonghui Tian Aihua Gao Pei Zhang Xinyuan Qi Zewei Luo Yixiang Duan Source Type: research

Hybridized nanolayer modified Ω-shaped fiber-optic synergistically enhances localized surface plasma resonance for ultrasensitive cytosensor and efficient photothermal therapy
Biosens Bioelectron. 2021 Aug 30;194:113599. doi: 10.1016/j.bios.2021.113599. Online ahead of print.ABSTRACTInadequate sensitivity and side-effect are the main challenges to develop cytosensors combining with therapeutic potential simultaneously for cancer diagnosis and treatment. Herein, localized surface plasma resonance (LSPR) based on hybridized nanolayer modified Ω-shaped fiber-optic (HN/Ω-FO) was developed to integrate cytosensor and plasmonic photothermal treatment (PPT). On one hand, hybridized nanolayers improve the coverage of nanoparticles and refractive index sensitivity (RIS). Moreover, the hybridi...
Source: Biosensors and Bioelectronics - September 14, 2021 Category: Biotechnology Authors: Lu He Fan He Yanting Feng Xu Wang Yongxin Li Yonghui Tian Aihua Gao Pei Zhang Xinyuan Qi Zewei Luo Yixiang Duan Source Type: research

Magnetic particle based liquid biopsy chip for isolation of extracellular vesicles and characterization by gene amplification
Biosens Bioelectron. 2021 Aug 27;194:113585. doi: 10.1016/j.bios.2021.113585. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are the cell-derived vesicles which play a critical role in cell-to-cell communication, and disease progression. These vesicles contain a myriad of substances like RNA, DNA, proteins, and lipids from their origin cells, offering a good source of biomarkers. The existing methods for the isolation of EVs are time-consuming, lack yield and purity, and expensive. In this work, we present a magnetic particle based liquid biopsy chip for the isolation of EVs by using a synthetic peptide, Vn96. ...
Source: Biosensors and Bioelectronics - September 13, 2021 Category: Biotechnology Authors: Srinivas Bathini Shanmugasundaram Pakkiriswami Rodney J Ouellette Anirban Ghosh Muthukumaran Packirisamy Source Type: research

Magnetic particle based liquid biopsy chip for isolation of extracellular vesicles and characterization by gene amplification
Biosens Bioelectron. 2021 Aug 27;194:113585. doi: 10.1016/j.bios.2021.113585. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are the cell-derived vesicles which play a critical role in cell-to-cell communication, and disease progression. These vesicles contain a myriad of substances like RNA, DNA, proteins, and lipids from their origin cells, offering a good source of biomarkers. The existing methods for the isolation of EVs are time-consuming, lack yield and purity, and expensive. In this work, we present a magnetic particle based liquid biopsy chip for the isolation of EVs by using a synthetic peptide, Vn96. ...
Source: Biosensors and Bioelectronics - September 13, 2021 Category: Biotechnology Authors: Srinivas Bathini Shanmugasundaram Pakkiriswami Rodney J Ouellette Anirban Ghosh Muthukumaran Packirisamy Source Type: research

Recent progress of self-powered respiration monitoring systems
Biosens Bioelectron. 2021 Sep 7;194:113609. doi: 10.1016/j.bios.2021.113609. Online ahead of print.ABSTRACTWearable and implantable medical devices are playing more and more key roles in disease diagnosis and health management. Various biosensors and systems have been used for respiration monitoring. Among them, self-powered sensors have some special characteristics such as low-cost, easy preparation, highly designable, and diversified. The respiratory airflow can drive the self-powered sensors directly to convert mechanical energy of the airflow into electricity. One of the major goals of the self-powered sensors and syst...
Source: Biosensors and Bioelectronics - September 12, 2021 Category: Biotechnology Authors: Jieyu Dai Linlin Li Bojing Shi Zhou Li Source Type: research

Editorial preface of the special issue on "the progress and perspectives of biosensing research in North America"
Biosens Bioelectron. 2021 Aug 24;194:113578. doi: 10.1016/j.bios.2021.113578. Online ahead of print.NO ABSTRACTPMID:34509166 | DOI:10.1016/j.bios.2021.113578 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - September 11, 2021 Category: Biotechnology Authors: Chen-Zhong Li Juewen Liu Koji Sode Source Type: research

Editorial preface of the special issue on "the progress and perspectives of biosensing research in North America"
Biosens Bioelectron. 2021 Aug 24;194:113578. doi: 10.1016/j.bios.2021.113578. Online ahead of print.NO ABSTRACTPMID:34509166 | DOI:10.1016/j.bios.2021.113578 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - September 11, 2021 Category: Biotechnology Authors: Chen-Zhong Li Juewen Liu Koji Sode Source Type: research

An indicator displacement assay-based optical chemosensor for heparin with a dual-readout and a reversible molecular logic gate operation based on the pyranine/methyl viologen
Biosens Bioelectron. 2021 Sep 3;194:113612. doi: 10.1016/j.bios.2021.113612. Online ahead of print.ABSTRACTWe have reported an optical indicator displacement assay (IDA) for heparin with a UV-vis absorbance and fluorescence dual-readout based on pyranine/methyl viologen (MV2+). Upon introducing heparin, pyranine/MV2+ shows a clearly observable increase in UV-vis absorbance and a turn-on of the fluorescence signal. We have demonstrated that the ionic nature of buffers significantly affects the pyranine displacement and the zwitterionic HEPES was most suitable for heparin sensing. After careful screening of experimental cond...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Zipin Zhang Rui Zhou Yongliang Dong Mengmeng Song Lei Tian Zuzhi Zhao Source Type: research

Multiplexed analysis of small extracellular vesicle-derived mRNAs by droplet digital PCR and machine learning improves breast cancer diagnosis
Biosens Bioelectron. 2021 Sep 4;194:113615. doi: 10.1016/j.bios.2021.113615. Online ahead of print.ABSTRACTBreast cancer has become the leading cause of global cancer incidence and a serious threat to women's health. Accurate diagnosis and early treatment are of great importance to prognosis. Although clinically used diagnostic approaches can be used for cancer screening, accurate diagnosis of breast cancer is still a critical unmet need. Here, we report a 4-plex droplet digital PCR technology for simultaneous detection of four small extracellular vesicle (sEV)-derived mRNAs (PGR, ESR1, ERBB2 and GAPDH) in combination with...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Chunchen Liu Bo Li Huixian Lin Chao Yang Jingyun Guo Binbin Cui Weilun Pan Junjie Feng Tingting Luo Fuxin Chu Xiaonan Xu Lei Zheng Shuhuai Yao Source Type: research

Sensitive electrochemical biosensor for Uracil-DNA glycosylase detection based on self-linkable hollow Mn/Ni layered doubled hydroxides as oxidase-like nanozyme for cascade signal amplification
Biosens Bioelectron. 2021 Sep 1;194:113607. doi: 10.1016/j.bios.2021.113607. Online ahead of print.ABSTRACTNanozymes have been widely used in biosensors instead of natural enzymes because of low cost, high stability and ease of storage. However, few works use oxidase-like nanozymes to fabricate electrochemical biosensors. Herein, we proposed a sensitive electrochemical biosensor to detect uracil-DNA glycosylase (UDG) based on the hollow Mn/Ni layered doubled hydroxides (h-Mn/Ni LDHs) as oxidase-like nanozyme. Briefly, the h-Mn/Ni LDHs, which was prepared by a facile hydrothermal method, exhibited excellent oxidase-like act...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Tingting Liu Zhiwen Li Mohan Chen Huijuan Zhao Zekun Zheng Lin Cui Xiaomei Zhang Source Type: research

Background-free sensing platform for on-site detection of carbamate pesticide through upconversion nanoparticles-based hydrogel suit
Biosens Bioelectron. 2021 Aug 28;194:113598. doi: 10.1016/j.bios.2021.113598. Online ahead of print.ABSTRACTOn-site monitoring of carbamate pesticide in complex matrix remians as a challenge in terms of the real-time control of food safety and supervision of environmental quality. Herein, we fabricated robust upconversion nanoparticles (UCNPS)/polydopamine (PDA)-based hydrogel portable suit that precisely quantified carbaryl in complex tea samples with smartphone detector. UCNPS/PDA nanoprobe was developed by polymerization of dopamine monomers on the surface of NaErF4: 0.5% Tm3+@NaYF4 through electrostatic interaction, le...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Dandan Su Xu Zhao Xu Yan Xiaosong Han Zhifang Zhu Chenguang Wang Xiaoteng Jia Fangmeng Liu Peng Sun Xiaomin Liu Geyu Lu Source Type: research

MRI magnetic compatible electrical neural interface: From materials to application
Biosens Bioelectron. 2021 Sep 1;194:113592. doi: 10.1016/j.bios.2021.113592. Online ahead of print.ABSTRACTNeural electrical interfaces are important tools for local neural stimulation and recording, which potentially have wide application in the diagnosis and treatment of neural diseases, as well as in the transmission of neural activity for brain-computer interface (BCI) systems. At the same time, magnetic resonance imaging (MRI) is one of the effective and non-invasive techniques for recording whole-brain signals, providing details of brain structures and also activation pattern maps. Simultaneous recording of extracell...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Yuan Zhang Song Le Hui Li Bowen Ji Ming-Hao Wang Jin Tao Jing-Qiu Liang Xiao-Yong Zhang Xiao-Yang Kang Source Type: research

Laser-induced graphene non-enzymatic glucose sensors for on-body measurements
Biosens Bioelectron. 2021 Sep 1;193:113606. doi: 10.1016/j.bios.2021.113606. Online ahead of print.ABSTRACTNon-enzymatic glucose sensors outperform enzymatic ones in terms of cost, sensitivity, stability, and operating duration. Though highly sensitive, it is still desirable to further improve the sensitivity of non-enzymatic glucose sensors to detect a trace amount of glucose in sweat and other biofluids. Among the demonstrated effective approaches using bimetals or 3D porous structures, the porous laser-induced graphene (LIG) on flexible polymers showcases good conductivity and a simple fabrication process for the integr...
Source: Biosensors and Bioelectronics - September 10, 2021 Category: Biotechnology Authors: Jia Zhu Shangbin Liu Zhihui Hu Xianzhe Zhang Ning Yi Kairui Tang Michael Gregory Dexheimer Xiaojun Lian Qing Wang Jian Yang Jennifer Gray Huanyu Cheng Source Type: research

Development of MIF/IL-1 β biosensors for discovery of critical quality attributes and potential allergic rhinitis targets from clinical real-world data by intelligent algorithm coupled with in vitro and vivo mechanism validation
Biosens Bioelectron. 2021 Sep 3;194:113608. doi: 10.1016/j.bios.2021.113608. Online ahead of print.ABSTRACTThere are still huge challenges from clinical real-world data to accurate targets and critical quality attributes (CQAs) for effective treatment of allergic rhinitis (AR). Here, we present a novel integrated strategy that biosensors and intelligent algorithms were used to angle AR targets and CQAs from clinical real world. Firstly, bagging and boosting partial least squares discrimination analysis (PLS-DA) and Monte-Carlo sampling were proposed to screen accurate AR targets. Macrophage migration inhibitory factor (MIF...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Lijuan Ma Yanfei Zheng Ji Wang Qianqian Li Jingqi Zeng Zijian Wang Tingjun Hou Yang Zhang Mingshuang Li Rongmin Shen Xuemei Chen Jingbo Qin Leting Lei Qing Xia Qi Wang Yanjiang Qiao Zhisheng Wu Source Type: research

Interspecific competition by non-exoelectrogenic Citrobacter freundii An1 boosts bioelectricity generation of exoelectrogenic Shewanella oneidensis MR-1
Biosens Bioelectron. 2021 Sep 3;194:113614. doi: 10.1016/j.bios.2021.113614. Online ahead of print.ABSTRACTThe performance of bioelectrochemical systems (BESs) is significantly influenced by metabolic interactions within a particular microbial community. Although some studies show that interspecific metabolic cooperation benefits BESs performance, the effect of interspecific substrate competition on BESs performance has not yet been discussed. Herein, the impact of interspecific competition is investigated by monitoring the extracellular electron transfer of exoelectrogenic Shewanella oneidensis MR-1 and non-exoelectrogeni...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Yong Xiao Geng Chen Zheng Chen Rui Bai Biyi Zhao Xiaochun Tian Yicheng Wu Xiao Zhou Feng Zhao Source Type: research

High-sensitivity and versatile plasmonic biosensor based on grain boundaries in polycrystalline 1L WS < sub > 2 < /sub > films
Biosens Bioelectron. 2021 Sep 3;194:113596. doi: 10.1016/j.bios.2021.113596. Online ahead of print.ABSTRACTStructural defects play an important role in exploitation of two-dimensional layered materials (2DLMs) for advanced biosensors with the increasingly high sensitivity and low detection limit. Grain boundaries (GBs), as an important type of structural defect in polycrystalline 2DLM films, potentially provide sufficient active defect sites for the immobilization of bioreceptor units via chemical functionalization. In this work, we report the selective functionalization of high-density GBs with complementary DNA receptors...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Lixuan Liu Kun Ye Zhiyan Jia Tianyu Xue Anmin Nie JianYong Xiang CongPu Mu Bochong Wang Fusheng Wen Kun Zhai Zhisheng Zhao Yongji Gong Zhongyuan Liu Yongjun Tian Source Type: research

Electrocatalytic CuBr@CuO nanoparticles based salivary glucose probes
Biosens Bioelectron. 2021 Sep 3;194:113610. doi: 10.1016/j.bios.2021.113610. Online ahead of print.ABSTRACTHighly electrocatalytic cuprous halide/copper oxide nanoparticles (CuX@CunO NPs; X = Cl, Br or I; n = 1 or 2) have been fabricated on copper foils for sensitive detection of glucose. Formation of CuX@CunO NPs involves two steps- in situ electrochemical deposition of CuX on the foil and then conversion of CuX to CunO. The deposited CuX converts to CunO, leading to the generation of abundant oxygen vacancies in the CuO lattice, enhancing the number of catalytically active sites, and improving the charge transfer efficie...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Wei-Jan Lin Yu-Syuan Lin Huan-Tsung Chang Binesh Unnikrishnan Chih-Ching Huang Source Type: research

A dual-signal electrochemiluminescence immunosensor for high-sensitivity detection of acute myocardial infarction biomarker
Biosens Bioelectron. 2021 Sep 3;194:113591. doi: 10.1016/j.bios.2021.113591. Online ahead of print.ABSTRACTBased on two different types of luminescence systems (Ru﹡(bpy)32+/TPA and SnO2 NFs/K2S2O8), a new type of electrochemiluminescence (ECL) immunosensor was prepared, which realized the detection of acute myocardial infarction biomarker cTnI. In this strategy, Ru(bpy)32+, above all, was immobilized on the NH2-MIL-125 as a capture probe. Subsequently, cTnI and SnO2 NFs was bonded to the electrode surface through the interaction between antigen and antibody in turn. During this process, Ru(bpy)32+ and the co-reactant TPA...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Chenghui Hong Pei Zhang Kening Lu Yu Ji Shuang He Dongqin Liu Nengqin Jia Source Type: research

Immunoassay based on Au-Ag bimetallic nanoclusters for colorimetric/fluorescent double biosensing of dicofol
Biosens Bioelectron. 2021 Sep 3;194:113611. doi: 10.1016/j.bios.2021.113611. Online ahead of print.ABSTRACTThe high toxicity of dicofol (DICO) to nontarget organisms has resulted in the contamination of food materials and caused a threat to human health. Developing a rapid and sensitive detection method of DICO in food samples is essential and still pursued. Fluorescent nanomaterials have been widely applied in biosensors to improve the sensitivity of detection. Herein, glutathione-capped Au-Ag bimetallic nanoclusters (Au-Ag NCs) exhibited the outstanding fluorescence characteristic with the average fluorescence lifetime o...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Yi Pan Xinlin Wei Xiaodong Guo Hui Wang Haiyun Song Canping Pan Naifeng Xu Source Type: research

Development of MIF/IL-1 β biosensors for discovery of critical quality attributes and potential allergic rhinitis targets from clinical real-world data by intelligent algorithm coupled with in vitro and vivo mechanism validation
Biosens Bioelectron. 2021 Sep 3;194:113608. doi: 10.1016/j.bios.2021.113608. Online ahead of print.ABSTRACTThere are still huge challenges from clinical real-world data to accurate targets and critical quality attributes (CQAs) for effective treatment of allergic rhinitis (AR). Here, we present a novel integrated strategy that biosensors and intelligent algorithms were used to angle AR targets and CQAs from clinical real world. Firstly, bagging and boosting partial least squares discrimination analysis (PLS-DA) and Monte-Carlo sampling were proposed to screen accurate AR targets. Macrophage migration inhibitory factor (MIF...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Lijuan Ma Yanfei Zheng Ji Wang Qianqian Li Jingqi Zeng Zijian Wang Tingjun Hou Yang Zhang Mingshuang Li Rongmin Shen Xuemei Chen Jingbo Qin Leting Lei Qing Xia Qi Wang Yanjiang Qiao Zhisheng Wu Source Type: research

Interspecific competition by non-exoelectrogenic Citrobacter freundii An1 boosts bioelectricity generation of exoelectrogenic Shewanella oneidensis MR-1
Biosens Bioelectron. 2021 Sep 3;194:113614. doi: 10.1016/j.bios.2021.113614. Online ahead of print.ABSTRACTThe performance of bioelectrochemical systems (BESs) is significantly influenced by metabolic interactions within a particular microbial community. Although some studies show that interspecific metabolic cooperation benefits BESs performance, the effect of interspecific substrate competition on BESs performance has not yet been discussed. Herein, the impact of interspecific competition is investigated by monitoring the extracellular electron transfer of exoelectrogenic Shewanella oneidensis MR-1 and non-exoelectrogeni...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Yong Xiao Geng Chen Zheng Chen Rui Bai Biyi Zhao Xiaochun Tian Yicheng Wu Xiao Zhou Feng Zhao Source Type: research

High-sensitivity and versatile plasmonic biosensor based on grain boundaries in polycrystalline 1L WS < sub > 2 < /sub > films
Biosens Bioelectron. 2021 Sep 3;194:113596. doi: 10.1016/j.bios.2021.113596. Online ahead of print.ABSTRACTStructural defects play an important role in exploitation of two-dimensional layered materials (2DLMs) for advanced biosensors with the increasingly high sensitivity and low detection limit. Grain boundaries (GBs), as an important type of structural defect in polycrystalline 2DLM films, potentially provide sufficient active defect sites for the immobilization of bioreceptor units via chemical functionalization. In this work, we report the selective functionalization of high-density GBs with complementary DNA receptors...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Lixuan Liu Kun Ye Zhiyan Jia Tianyu Xue Anmin Nie JianYong Xiang CongPu Mu Bochong Wang Fusheng Wen Kun Zhai Zhisheng Zhao Yongji Gong Zhongyuan Liu Yongjun Tian Source Type: research

Electrocatalytic CuBr@CuO nanoparticles based salivary glucose probes
Biosens Bioelectron. 2021 Sep 3;194:113610. doi: 10.1016/j.bios.2021.113610. Online ahead of print.ABSTRACTHighly electrocatalytic cuprous halide/copper oxide nanoparticles (CuX@CunO NPs; X = Cl, Br or I; n = 1 or 2) have been fabricated on copper foils for sensitive detection of glucose. Formation of CuX@CunO NPs involves two steps- in situ electrochemical deposition of CuX on the foil and then conversion of CuX to CunO. The deposited CuX converts to CunO, leading to the generation of abundant oxygen vacancies in the CuO lattice, enhancing the number of catalytically active sites, and improving the charge transfer efficie...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Wei-Jan Lin Yu-Syuan Lin Huan-Tsung Chang Binesh Unnikrishnan Chih-Ching Huang Source Type: research

A dual-signal electrochemiluminescence immunosensor for high-sensitivity detection of acute myocardial infarction biomarker
Biosens Bioelectron. 2021 Sep 3;194:113591. doi: 10.1016/j.bios.2021.113591. Online ahead of print.ABSTRACTBased on two different types of luminescence systems (Ru﹡(bpy)32+/TPA and SnO2 NFs/K2S2O8), a new type of electrochemiluminescence (ECL) immunosensor was prepared, which realized the detection of acute myocardial infarction biomarker cTnI. In this strategy, Ru(bpy)32+, above all, was immobilized on the NH2-MIL-125 as a capture probe. Subsequently, cTnI and SnO2 NFs was bonded to the electrode surface through the interaction between antigen and antibody in turn. During this process, Ru(bpy)32+ and the co-reactant TPA...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Chenghui Hong Pei Zhang Kening Lu Yu Ji Shuang He Dongqin Liu Nengqin Jia Source Type: research

Immunoassay based on Au-Ag bimetallic nanoclusters for colorimetric/fluorescent double biosensing of dicofol
Biosens Bioelectron. 2021 Sep 3;194:113611. doi: 10.1016/j.bios.2021.113611. Online ahead of print.ABSTRACTThe high toxicity of dicofol (DICO) to nontarget organisms has resulted in the contamination of food materials and caused a threat to human health. Developing a rapid and sensitive detection method of DICO in food samples is essential and still pursued. Fluorescent nanomaterials have been widely applied in biosensors to improve the sensitivity of detection. Herein, glutathione-capped Au-Ag bimetallic nanoclusters (Au-Ag NCs) exhibited the outstanding fluorescence characteristic with the average fluorescence lifetime o...
Source: Biosensors and Bioelectronics - September 9, 2021 Category: Biotechnology Authors: Yi Pan Xinlin Wei Xiaodong Guo Hui Wang Haiyun Song Canping Pan Naifeng Xu Source Type: research

Flow radiocytometry using droplet optofluidics
Biosens Bioelectron. 2021 Aug 13;194:113565. doi: 10.1016/j.bios.2021.113565. Online ahead of print.ABSTRACTFlow-based cytometry methods are widely used to analyze heterogeneous cell populations. However, their use for small molecule studies remains limited due to bulky fluorescent labels that often interfere with biochemical activity in cells. In contrast, radiotracers require minimal modification of their target molecules and can track biochemical processes with negligible interference and high specificity. Here, we introduce flow radiocytometry (FRCM) that broadens the scope of current cytometry methods to include beta-...
Source: Biosensors and Bioelectronics - September 7, 2021 Category: Biotechnology Authors: Byunghang Ha Tae Jin Kim Ejung Moon Amato J Giaccia Guillem Pratx Source Type: research

Flow radiocytometry using droplet optofluidics
Biosens Bioelectron. 2021 Aug 13;194:113565. doi: 10.1016/j.bios.2021.113565. Online ahead of print.ABSTRACTFlow-based cytometry methods are widely used to analyze heterogeneous cell populations. However, their use for small molecule studies remains limited due to bulky fluorescent labels that often interfere with biochemical activity in cells. In contrast, radiotracers require minimal modification of their target molecules and can track biochemical processes with negligible interference and high specificity. Here, we introduce flow radiocytometry (FRCM) that broadens the scope of current cytometry methods to include beta-...
Source: Biosensors and Bioelectronics - September 7, 2021 Category: Biotechnology Authors: Byunghang Ha Tae Jin Kim Ejung Moon Amato J Giaccia Guillem Pratx Source Type: research

Flow radiocytometry using droplet optofluidics
Biosens Bioelectron. 2021 Aug 13;194:113565. doi: 10.1016/j.bios.2021.113565. Online ahead of print.ABSTRACTFlow-based cytometry methods are widely used to analyze heterogeneous cell populations. However, their use for small molecule studies remains limited due to bulky fluorescent labels that often interfere with biochemical activity in cells. In contrast, radiotracers require minimal modification of their target molecules and can track biochemical processes with negligible interference and high specificity. Here, we introduce flow radiocytometry (FRCM) that broadens the scope of current cytometry methods to include beta-...
Source: Biosensors and Bioelectronics - September 7, 2021 Category: Biotechnology Authors: Byunghang Ha Tae Jin Kim Ejung Moon Amato J Giaccia Guillem Pratx Source Type: research

A disposable, wearable, flexible, stitched textile electrochemical biosensing platform
Biosens Bioelectron. 2021 Sep 1;194:113604. doi: 10.1016/j.bios.2021.113604. Online ahead of print.ABSTRACTWearable sensors are a fast growing and exciting research area, the success of smart watches are a great example of the utility and demand for wearable sensing systems. The current state of the art routinely uses expensive and bulky equipment designed for long term use. There is a need for cheap and disposable wearable sensors to make single use measurements, primarily in the area of biomarker detection. Herein we report the ability to make cheap (0.22 USD/sensor), disposable, wearable sensors by stitching conductive ...
Source: Biosensors and Bioelectronics - September 6, 2021 Category: Biotechnology Authors: Andrew Piper Ingrid Öberg Månsson Shirin Khaliliazar Roman Landin Mahiar Max Hamedi Source Type: research

Dual photonic bandgap hollow sphere colloidal photonic crystals for real-time fluorescence enhancement in living cells
Biosens Bioelectron. 2021 Aug 27;194:113577. doi: 10.1016/j.bios.2021.113577. Online ahead of print.ABSTRACTTo overcome the problems of refractive index matching and increased disorder when working with traditional heterostructure colloidal photonic crystals (CPCs) with dual or multiple photonic bandgaps (PBGs) for fluorescence enhancement in water, we propose the use of a chemical heterostructure in hollow sphere CPCs (HSCPCs). A partial chemical modification of the HSCPC creates a large contrast in wettability to induce the heterostructure, while the hollow spheres increase the refractive index difference when used in aq...
Source: Biosensors and Bioelectronics - September 4, 2021 Category: Biotechnology Authors: Kuo Zhong Wei Yu Yovan de Coene Atsushi Yamada Olga Krylychkina Stijn Jooken Olivier Deschaume Carmen Bartic Koen Clays Source Type: research

A DNA-derived phage nose using machine learning and artificial neural processing for diagnosing lung cancer
Biosens Bioelectron. 2021 Aug 14;194:113567. doi: 10.1016/j.bios.2021.113567. Online ahead of print.ABSTRACTThere is a growing interest in electronic nose-based diagnostic systems that are fast and portable. However, existing technologies are suitable only for operation in the laboratory, making them difficult to apply in a rapid, non-face-to-face, and field-suitable manner. Here, we demonstrate a DNA-derived phage nose (D2pNose) as a portable respiratory disease diagnosis system requiring no pretreatment. D2pNose was produced based on phage colour films implanted with DNA sequences from mammalian olfactory receptor cells,...
Source: Biosensors and Bioelectronics - September 4, 2021 Category: Biotechnology Authors: Jong-Min Lee Eun Jeong Choi Jae Heun Chung Ki-Wook Lee Yujin Lee Ye-Ji Kim Won-Geun Kim Seong Hoon Yoon Hee Yun Seol Vasanthan Devaraj Jong Seong Ha Donghan Lee Sang-Mo Kwon Yun Seong Kim Chulhun L Chang Jin-Woo Oh Source Type: research

Development of antibody against drug-resistant respiratory syncytial virus: Rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system
Biosens Bioelectron. 2021 Aug 26;194:113593. doi: 10.1016/j.bios.2021.113593. Online ahead of print.ABSTRACTRespiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furtherm...
Source: Biosensors and Bioelectronics - September 4, 2021 Category: Biotechnology Authors: Hyeran Kim Seul Gee Hwang Kyeonghye Guk Yoonji Bae Hwangseo Park Eun-Kyung Lim Taejoon Kang Juyeon Jung Source Type: research

Nanopore detects γ-radiation inhibited HIV-1 protease activity
Biosens Bioelectron. 2021 Aug 30;194:113602. doi: 10.1016/j.bios.2021.113602. Online ahead of print.ABSTRACTInhibition of HIV-1 protease (PR) activity is realized by exposure to 60Co γ-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows ~50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of γ-radiation (0.1K Gy), and the values reach to over 90% with high γ-ray treatme...
Source: Biosensors and Bioelectronics - September 4, 2021 Category: Biotechnology Authors: Minghan Li Wei Li Yicen Xiao Qianshan Liu Liyuan Liang Deqiang Wang Wenli Huang Liang Wang Source Type: research

In situ growth of TiO < sub > 2 < /sub > nanowires on Ti < sub > 3 < /sub > C < sub > 2 < /sub > MXenes nanosheets as highly sensitive luminol electrochemiluminescent nanoplatform for glucose detection in fruits, sweat and serum samples
Biosens Bioelectron. 2021 Aug 28;194:113600. doi: 10.1016/j.bios.2021.113600. Online ahead of print.ABSTRACTA sensitive electrogenerated chemiluminescence (ECL) biosensor for glucose was developed based on in situ growth of TiO2 nanowires on Ti3C2 MXenes (TiO2-Ti3C2) as the nanoplatform. Via tuning the alkaline oxidation time, different amount of TiO2 nanowires can be found on MXenes. An ECL biosensor for glucose was constructed by covalent immobilization of glucose oxidase (GODx) on the glycine functional TiO2-Ti3C2 surface, with the ECL signal depending on the in-situ formation of H2O2 via the specifically catalysis of g...
Source: Biosensors and Bioelectronics - September 4, 2021 Category: Biotechnology Authors: Yan Sun Peipei Li Yu Zhu Xiaohua Zhu Youyu Zhang Meiling Liu Yang Liu Source Type: research

Online and offline preconcentration techniques on paper-based analytical devices for ultrasensitive chemical and biochemical analysis: A review
Biosens Bioelectron. 2021 Aug 20;194:113574. doi: 10.1016/j.bios.2021.113574. Online ahead of print.ABSTRACTMicrofluidic paper-based analytical devices (μPADs) have attracted much attention over the past decade. They embody many advantages, such as abundance, portability, cost-effectiveness, and ease of fabrication, making them superior for clinical diagnostics, environmental monitoring, and food safety assurance. Despite these advantages, μPADs lack the high sensitivity to detect many analytes at trace levels than other commercial analytical instruments such as mass spectrometry. Therefore, a preconcentration step i...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Waleed Alahmad Ali Sahragard Pakorn Varanusupakul Source Type: research

Lab-on-a-disc for ultrafast plasmonic assay of cysteamine
Biosens Bioelectron. 2021 Aug 24;194:113584. doi: 10.1016/j.bios.2021.113584. Online ahead of print.ABSTRACTCysteamine (CA) is a cystine depleting agent used in the treatment of cystinosis and many other diseases. However, high dose of CA can be toxic and thus point-of-care-test devices measuring blood CA level can be highly beneficial. Here, we report a highly sensitive, straightforward, and quantitative assay for the colorimetric and spectroscopic determination of CA concentration using plasmonic nanoparticles. The principle is based on the chemical etching-induced exchange of the surface ligands of plasmonic gold nanopa...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Mamata Karmacharya Sumit Kumar Chaeeun Lee Yoon-Kyoung Cho Source Type: research

Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor
Biosens Bioelectron. 2021 Aug 28;194:113588. doi: 10.1016/j.bios.2021.113588. Online ahead of print.ABSTRACTUltrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal s...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Mohammad Julker Neyen Sampad Han Zhang Thomas D Yuzvinsky Matthew A Stott Aaron R Hawkins Holger Schmidt Source Type: research

Decentralized vitamin C & amp; D dual biosensor chip: Toward personalized immune system support
Biosens Bioelectron. 2021 Aug 26;194:113590. doi: 10.1016/j.bios.2021.113590. Online ahead of print.ABSTRACTCombating the ongoing COVID-19 pandemic has put the spotlight on nutritional support of the immune system through consumption of vitamins C and D. Accordingly, there are urgent demands for an effective on-the-spot multi-vitamin self-testing platform that monitors the levels of these immune-supporting micronutrients for guiding precision nutrition recommendations. Herein, we present a compact bioelectronic dual sensor chip aimed at frequent on-the-spot simultaneous monitoring of the salivary vitamin C and D dynamics. ...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: V íctor Ruiz-Valdepeñas Montiel Juliane R Sempionatto Eva Vargas Eileen Bailey Jennifer May Andrea Bulbarello Andr é Düsterloh Nathan Matusheski Joseph Wang Source Type: research

Thiol-sensitive probe enables dynamic electrochemical assembly of serum protein for detecting SARS-Cov-2 marker protease in clinical samples
Biosens Bioelectron. 2021 Aug 24;194:113579. doi: 10.1016/j.bios.2021.113579. Online ahead of print.ABSTRACTThe poor situational awareness about the spreading of the virus especially in the underdeveloped regions calls for novel virus assays of low cost and simple operation. Currently, such assays are exclusively restricted to nucleic acid detection. In this investigation, a virus protein serum assay has been proposed in a one-step and reagent-less route. Specifically, in this assay, the main protease of the virus is targeted by a short probe mimicking its substrate. While the probe-protein interaction brings them together...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Kai Zhang Zhenqiang Fan Yuedi Ding Jinlong Li Hao Li Source Type: research

ExoSD chips for high-purity immunomagnetic separation and high-sensitivity detection of gastric cancer cell-derived exosomes
Biosens Bioelectron. 2021 Aug 27;194:113594. doi: 10.1016/j.bios.2021.113594. Online ahead of print.ABSTRACTGastric cancer cell-derived exosomes as biomarkers have a very high application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30-150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). Here we proposed a highly integrated exosome separation and detection (ExoSD) chip to immunomagnetic separate exosomes from cell culture supernatant in a manner of continuous flow, an...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Zixian Yu Shujing Lin Fangfang Xia Yanlei Liu Di Zhang Fei Wang Yanpu Wang Qichao Li Jiaqi Niu Chengxi Cao Daxiang Cui Nengquan Sheng Jiazi Ren Zhigang Wang Di Chen Source Type: research

Simultaneous detection of multiple neuroendocrine tumor markers in patient serum with an ultrasensitive and antifouling electrochemical immunosensor
Biosens Bioelectron. 2021 Aug 30;194:113603. doi: 10.1016/j.bios.2021.113603. Online ahead of print.ABSTRACTNeuroendocrine tumors (NETs) are rare heterogeneous tumors that are often misdiagnosed and mistreated. Most NETs patients are diagnosed as advanced. Early on-time detection of NETs is significant for precision therapy. Here, an ultrasensitive and antifouling label-free electrochemical immunosensor was constructed for simultaneous analysis of NETs biomarkers chromogranin A (CgA) and chromogranin B (CgB). The metal ion functionalized porous magnesium silicate/gold nanoparticles/polyethylene glycol/chitosan (PMS-M2+/AuN...
Source: Biosensors and Bioelectronics - September 2, 2021 Category: Biotechnology Authors: Xuejiao Liu Yuanliang Li Li He Yongjun Feng Huangying Tan Xu Chen Wensheng Yang Source Type: research

Accurate and efficient intracellular delivery biosensing system by nanostrawed electroporation array
Biosens Bioelectron. 2021 Aug 24;194:113583. doi: 10.1016/j.bios.2021.113583. Online ahead of print.ABSTRACTElectroporation serves as a powerful technique to introduce the exogenous nucleotides, DNA, RNA, proteins, dyes, and virus particles into cells. Through the effect of high intensity of electric field, the permeability of the cell membrane is instantaneously improved to absorb the exogenous molecules in surrounding medium. To protect the cell viability, ultralow-voltage electroporation techniques are well developed by versatile devices, and delivery efficiency is commonly assessed by label-based analysis by microscope...
Source: Biosensors and Bioelectronics - August 31, 2021 Category: Biotechnology Authors: Jiaru Fang Jiarong Xu Yuting Xiang Hongbo Li Dongxin Xu Xi Xie Ning Hu Source Type: research

Efficient and robust dual modes of fluorescence sensing and smartphone readout for the detection of pyrethroids using artificial receptors bound inside a covalent organic framework
Biosens Bioelectron. 2021 Aug 25;194:113582. doi: 10.1016/j.bios.2021.113582. Online ahead of print.ABSTRACTIn the study, we have developed an efficient and robust method using dual modes of fluorescence sensing and smartphone readout for the detection of pyrethroids using artificial receptors inside a covalent organic framework. Carbazole-conjugated frameworks (CCFs) were used to prepare efficient fluorescent probes that combine stability with light-emitting activity. CN linkages between aldehydes and amines formed Schiff bases, allowing the development of layered structures, creating exceptionally stable frameworks. Arti...
Source: Biosensors and Bioelectronics - August 30, 2021 Category: Biotechnology Authors: Wei Jiang Yuan Zhao Dianwei Zhang Xuecheng Zhu Huilin Liu Baoguo Sun Source Type: research

Flexible photoelectrochemical biosensor for ultrasensitive microRNA detection based on concatenated multiplex signal amplification
Biosens Bioelectron. 2021 Aug 25;194:113581. doi: 10.1016/j.bios.2021.113581. Online ahead of print.ABSTRACTPrecise microRNA (miRNA) analysis is significant importance for early disease diagnosis. Herein, a novel flexible photoelectrochemical (PEC) biosensor for miRNA determination was developed by employing CdS NPs-modified carbon cloth (CC) on polyimide (PI) film as photoelectric material to provide the PEC responses and an efficient four-stage reaction system as the target recognition and signal amplification unit to improve the analytical performance. In this PEC biosensor, the presence of target miR-21 would trigger t...
Source: Biosensors and Bioelectronics - August 30, 2021 Category: Biotechnology Authors: Yuecan Zhao Junzhu Xiang Hao Cheng Xiaojuan Liu Feng Li Source Type: research

Automated analysis of human cardiomyocytes dynamics with holographic image-based tracking for cardiotoxicity screening
Biosens Bioelectron. 2021 Aug 23;195:113570. doi: 10.1016/j.bios.2021.113570. Online ahead of print.ABSTRACTThis paper proposes a new non-invasive, low-cost, and fully automated platform to quantitatively analyze dynamics of human-induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) at the single-cell level by holographic image-based tracking for cardiotoxicity screening. A dense Farneback optical flow method and holographic imaging informatics were combined to characterize the contractile motion of a single CM, which obviates the need for costly equipment to monitor a CM's mechanical beat activity. The reliabil...
Source: Biosensors and Bioelectronics - August 29, 2021 Category: Biotechnology Authors: Ezat Ahamadzadeh Keyvan Jaferzadeh Seonghwan Park Seungwoo Son Inkyu Moon Source Type: research

Thermal self-regulatory intelligent biosensor based on carbon-nanotubes-decorated phase-change microcapsules for enhancement of glucose detection
This study provides a promising strategy for the development of thermal self-regulatory smart biosensors with an enhanced identification ability to detect various chemical substances over a wide range of applicable temperatures.PMID:34455144 | DOI:10.1016/j.bios.2021.113586 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - August 29, 2021 Category: Biotechnology Authors: Zhao Sun Huan Liu Xiaodong Wang Source Type: research

CRISPR/Cas12a collateral cleavage activity for simple and rapid detection of protein/small molecule interaction
Biosens Bioelectron. 2021 Aug 25;194:113587. doi: 10.1016/j.bios.2021.113587. Online ahead of print.ABSTRACTTo realize the full potential of the CRISPR/Cas system and expand its applicability up to the detection of molecular interactions, we herein describe a novel method to identify protein/small molecule interactions by utilizing the CRISPR/Cas12a collateral cleavage activity. This technique employs a single-stranded activator DNA modified with a specific small molecule, which would switch on the CRISPR/Cas12a collateral cleavage activity upon binding to crRNA within the CRISPR/Cas12a system. When the target protein bind...
Source: Biosensors and Bioelectronics - August 29, 2021 Category: Biotechnology Authors: Hansol Kim Seoyoung Lee Junhyeok Yoon Jayeon Song Hyun Gyu Park Source Type: research

Photoelectrochemical biosensor for DNA hydroxymethylation detection based on the enhanced photoactivity of in-situ synthesized Bi < sub > 4 < /sub > NbO < sub > 8 < /sub > Cl@Bi < sub > 2 < /sub > S < sub > 3 < /sub > heterojunction
Biosens Bioelectron. 2021 Aug 24;194:113580. doi: 10.1016/j.bios.2021.113580. Online ahead of print.ABSTRACTAs an important epigenetic modification, 5-hydroxymethylcytosine (5hmC) aroused wide concern about the distribution and the function. Due to the necessity of 5hmC detection, a novel photoelectrochemical (PEC) biosensor was established based on the in-situ generated heterojunction of Bi4NbO8Cl@Bi2S3, which was employed as the substrate material with excellent photoelectric property. The specific recognition of 5hmC relied on the covalent reaction between -CH2OH of 5hmC and -SH on the substrate electrode under the cata...
Source: Biosensors and Bioelectronics - August 28, 2021 Category: Biotechnology Authors: Jia Ding Yunlei Zhou Qian Wang Shiyun Ai Source Type: research