Aggregation-induced emission of TTCPy-3: A novel approach for eradicating Nocardia seriolae infections in aquatic fishes
This study introduces luminogens with aggregation-induced emission (AIE) as an innovative and non-antibiotic therapy for N. seriolae. Specifically, the AIE photosensitizer, TTCPy-3 is employed against N. seriolae. We evaluated the antibacterial activity of TTCPy-3 and investigated the killing mechanism against N. seriolae, emphasizing its ability to aggregate within the bacterium and produce reactive oxygen species (ROS). TTCPy-3 could effectively aggregate in N. seriolae, generate ROS, and perform real-time imaging of the bacteria. A bactericidal efficiency of 100% was observed while concentrations exceeding 4 μM in the ...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Zhigang Liu Junling Zhang Xiaona Ma Miao Wang Lijin Jiang Meiyan Zhang Maixin Lu Ouqin Chang Jianmeng Cao Xiaoli Ke Mengmeng Yi Source Type: research

Label-free plasmonic spectral profiling of serum DNA
Biosens Bioelectron. 2024 Mar 13;254:116199. doi: 10.1016/j.bios.2024.116199. Online ahead of print.ABSTRACTGenetic and epigenetic modifications are linked to the activation of oncogenes and inactivation of tumor suppressor genes. Likewise, the associated molecular alternations can best inform precision medicine for personalized tumor treatment. Therefore, performing characterization of genetic and epigenetic alternations at the molecular level represents a crucial step in early diagnosis and/or therapeutics of cancer. However, the prevailing methods for DNA analysis involve a series of tedious and complicated steps, in wh...
Source: Biosensors and Bioelectronics - March 16, 2024 Category: Biotechnology Authors: Peng Zheng Piyush Raj Le Liang Lintong Wu Santosh Kumar Paidi Jeong Hee Kim Ishan Barman Source Type: research

Integrating target-responsive microfluidic-based biosensing chip with smartphone for simultaneous quantification of multiple fluoroquinolones
In this study, the background-free properties of upconversion nanoparticles (UCNPs), the high specificity of the target aptamer (Apt), and the high quenching properties of graphene oxide (GO) were integrated into a microfluidic-based fluorescence biosensing chip (MFBC). Interestingly, the microfluidic channels of the MFBC were prepared by laser-printing technology without the need for complex preparation processes and additional specialized equipment. The target-responsive fluorescence biosensing probes loaded on the MFBC were prepared by self-assembly of the UCNPs-Apt complex with GO based on π-π stacking interactions, ...
Source: Biosensors and Bioelectronics - March 15, 2024 Category: Biotechnology Authors: Mingming Zhang Xue Wang Shuangshuang Liu Tahreem Riaz Quansheng Chen Qin Ouyang Source Type: research

A point-of-care electrochemical biosensor for the rapid and sensitive detection of biomarkers in murine models with LPS-induced sepsis
This study proposes a point-of-care (POC) electrochemical biosensor that overcomes the limitations of current biosensing technologies in the clinical setting by its integration with electrokinetics, enhancing the sensitivity to picogram level compared with the nanogram limit of current diagnostic technologies. This biosensor promotes the use of a microelectrode strip to address the limitations of conventional photolithographic fabrication methods. Tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and microRNA-155 (miR-155) were monitored in a lipopolysaccharide (LPS)-induced septic mouse model. The optimum target...
Source: Biosensors and Bioelectronics - March 15, 2024 Category: Biotechnology Authors: Neil Adrian P Ondevilla Peng-Wen Liu Wan-Ting Huang Tzu-Ping Weng Nan-Yao Lee Syu-Cing Ma Jian-Jang Huang Tak-Wah Wong Hsien-Chang Chang Source Type: research

Fluorescent responsive membrane based on terbium coordination polymer and carbon dots with AIE effect for rapid and visual detection of fluoroquinolone
In this study, based on aggregation-induced emission (AIE) effect and antenna effect, a novel portable fluorescent responsive membrane was constructed with red carbon dots (R-CDs) as reference signal and terbium coordination polymer (Tb-AMP CPs) as response signal for visual, instrument-free, and sensitive detection of fluoroquinolones (FQs). Specifically, the fluorescent responsive membrane (R-T membrane) was prepared by physically depositing R-CDs with AIE property and Tb-AMP CPs on the surface of polyvinylidene fluoride filter membranes at ambient temperature. In the presence of FQs, Tb3+ in the Tb-AMP CPs of the prepar...
Source: Biosensors and Bioelectronics - March 14, 2024 Category: Biotechnology Authors: Yunyun Hu Xi Chen Kai Wang Chuang Jiang Wenya Liu Siyu Zhang Mingming Zheng Yibin Zhou Yaqing Xiao Yingnan Liu Source Type: research

Highly catalytic Prussian blue analogues and their application on the three-dimensional origami paper-based sweat sensors
This study utilizes a simple one-pot synthesis approach to produce a tungsten-disulfide (WS2) and iron-cobalt Prussian blue analogue composite (WS2-PBA), enhancing conductivity and electron transfer rate performance. Through the inclusion of sodium citrate into the solution, the S-edge site concentration of WS2 increases. This augmentation introduces additional active sites and defects into the catalyst, enhancing its catalytic activity. The effectiveness of the WS2-PBA 3D-Origami paper device for lactate detection in sweat is also evaluated for biomedical applications. The device demonstrated a robust relationship between...
Source: Biosensors and Bioelectronics - March 14, 2024 Category: Biotechnology Authors: Wei-Ting Chen Chi-Fong Yan Cheng-Ju Yu Ying-Chih Liao Chien-Fu Chen Source Type: research

Fluorescent responsive membrane based on terbium coordination polymer and carbon dots with AIE effect for rapid and visual detection of fluoroquinolone
In this study, based on aggregation-induced emission (AIE) effect and antenna effect, a novel portable fluorescent responsive membrane was constructed with red carbon dots (R-CDs) as reference signal and terbium coordination polymer (Tb-AMP CPs) as response signal for visual, instrument-free, and sensitive detection of fluoroquinolones (FQs). Specifically, the fluorescent responsive membrane (R-T membrane) was prepared by physically depositing R-CDs with AIE property and Tb-AMP CPs on the surface of polyvinylidene fluoride filter membranes at ambient temperature. In the presence of FQs, Tb3+ in the Tb-AMP CPs of the prepar...
Source: Biosensors and Bioelectronics - March 14, 2024 Category: Biotechnology Authors: Yunyun Hu Xi Chen Kai Wang Chuang Jiang Wenya Liu Siyu Zhang Mingming Zheng Yibin Zhou Yaqing Xiao Yingnan Liu Source Type: research

Highly catalytic Prussian blue analogues and their application on the three-dimensional origami paper-based sweat sensors
This study utilizes a simple one-pot synthesis approach to produce a tungsten-disulfide (WS2) and iron-cobalt Prussian blue analogue composite (WS2-PBA), enhancing conductivity and electron transfer rate performance. Through the inclusion of sodium citrate into the solution, the S-edge site concentration of WS2 increases. This augmentation introduces additional active sites and defects into the catalyst, enhancing its catalytic activity. The effectiveness of the WS2-PBA 3D-Origami paper device for lactate detection in sweat is also evaluated for biomedical applications. The device demonstrated a robust relationship between...
Source: Biosensors and Bioelectronics - March 14, 2024 Category: Biotechnology Authors: Wei-Ting Chen Chi-Fong Yan Cheng-Ju Yu Ying-Chih Liao Chien-Fu Chen Source Type: research

Multi-scale bioimpedance flexible sensing with causal hierarchical machine learning for fish vitality evaluation under adversity stress
In this study, we developed a laser-activated, stretchable, highly conductive liquid metal (LM) based flexible sensor system for fish multi-scale bioimpedance detection. It has excellent conformability, electrical conductivity, bending and cyclic tensile stability. Meanwhile, test result showed that wireless power supply is a potential solution for realizing safe power supply for devices inside waterless low-temperature packages. In addition, a hierarchical regression model (GC-HRM) based on Granger causality was established. The result showed that tissue bioimpedance can induce changes in individual bioimpedance with unid...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Luwei Zhang Chuiyu Kong You Li Yanfu He Xiangyun Guo Dongjie Shi Xiaoshuan Zhang Jinyou Hu Source Type: research

Development of highly sensitive dual-enhanced fluorescence quenching immunochromatographic test strips based on Pt nanoprobes
Biosens Bioelectron. 2024 Mar 6;254:116195. doi: 10.1016/j.bios.2024.116195. Online ahead of print.ABSTRACTThe fluorescence-quenching method is crucial in vitro analysis, particularly for immunochromatographic test strips (ICTs) using noble metal nanoparticles as probes. However, ICTs still fall short in meeting the requirements for the detection of traces biomarkers due to the noble metal nanoparticles can only quench fluorescence of the dyes within a confined distance. Interestingly, noble metal nanoparticles, such as Pt NPs cannot only perform fluorescence-quenching ability based on the Förster resonance energy transfe...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Jiashuo Xu Shuang Zhao Qiuting Zhang Xu Huang Kang Du Jinzhi Wang Jiaxun Wang Cheng Chen Bingbo Zhang Jin Chang Xiaoqun Gong Source Type: research

Polymerized carbon dots with high electrochemiluminescence efficiency and long wavelength ECL emission for ultrasensitive detection of MicroRNA-222
Biosens Bioelectron. 2024 Mar 5;254:116193. doi: 10.1016/j.bios.2024.116193. Online ahead of print.ABSTRACTHerein, a new electrochemiluminescence (ECL) biosensor was constructed with highly efficient polymerized carbon dots (PCDs) as ECL emitter and the improved localized catalytic hairpin assembly (L-CHA) as signal amplifier for ultrasensitive detection of microRNA-222 (miRNA-222). Impressively, compared to the traditional carbon dots with inefficient blue region ECL emission, PCDs with N, O co-dope and large conjugated π-system showed high electrical conductivity, narrow band gap and strong radiative transition, which c...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Shuang Xiao Yu-Ting Yang Yi-Fei Chen Jia-Li Liu Ya-Qin Chai Ruo Yuan Source Type: research

Multi-scale bioimpedance flexible sensing with causal hierarchical machine learning for fish vitality evaluation under adversity stress
In this study, we developed a laser-activated, stretchable, highly conductive liquid metal (LM) based flexible sensor system for fish multi-scale bioimpedance detection. It has excellent conformability, electrical conductivity, bending and cyclic tensile stability. Meanwhile, test result showed that wireless power supply is a potential solution for realizing safe power supply for devices inside waterless low-temperature packages. In addition, a hierarchical regression model (GC-HRM) based on Granger causality was established. The result showed that tissue bioimpedance can induce changes in individual bioimpedance with unid...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Luwei Zhang Chuiyu Kong You Li Yanfu He Xiangyun Guo Dongjie Shi Xiaoshuan Zhang Jinyou Hu Source Type: research

Development of highly sensitive dual-enhanced fluorescence quenching immunochromatographic test strips based on Pt nanoprobes
Biosens Bioelectron. 2024 Mar 6;254:116195. doi: 10.1016/j.bios.2024.116195. Online ahead of print.ABSTRACTThe fluorescence-quenching method is crucial in vitro analysis, particularly for immunochromatographic test strips (ICTs) using noble metal nanoparticles as probes. However, ICTs still fall short in meeting the requirements for the detection of traces biomarkers due to the noble metal nanoparticles can only quench fluorescence of the dyes within a confined distance. Interestingly, noble metal nanoparticles, such as Pt NPs cannot only perform fluorescence-quenching ability based on the Förster resonance energy transfe...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Jiashuo Xu Shuang Zhao Qiuting Zhang Xu Huang Kang Du Jinzhi Wang Jiaxun Wang Cheng Chen Bingbo Zhang Jin Chang Xiaoqun Gong Source Type: research

Polymerized carbon dots with high electrochemiluminescence efficiency and long wavelength ECL emission for ultrasensitive detection of MicroRNA-222
Biosens Bioelectron. 2024 Mar 5;254:116193. doi: 10.1016/j.bios.2024.116193. Online ahead of print.ABSTRACTHerein, a new electrochemiluminescence (ECL) biosensor was constructed with highly efficient polymerized carbon dots (PCDs) as ECL emitter and the improved localized catalytic hairpin assembly (L-CHA) as signal amplifier for ultrasensitive detection of microRNA-222 (miRNA-222). Impressively, compared to the traditional carbon dots with inefficient blue region ECL emission, PCDs with N, O co-dope and large conjugated π-system showed high electrical conductivity, narrow band gap and strong radiative transition, which c...
Source: Biosensors and Bioelectronics - March 13, 2024 Category: Biotechnology Authors: Shuang Xiao Yu-Ting Yang Yi-Fei Chen Jia-Li Liu Ya-Qin Chai Ruo Yuan Source Type: research

One-step construction of multiplexed enzymatic biosensors using light-addressable electrochemistry on a single silicon photoelectrode
Biosens Bioelectron. 2024 Mar 6;253:116194. doi: 10.1016/j.bios.2024.116194. Online ahead of print.ABSTRACTThe multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy involving the immobilization of both redox mediators and enzymes using an amide bond-based hydrogel membrane was propos...
Source: Biosensors and Bioelectronics - March 11, 2024 Category: Biotechnology Authors: Qiaoyu Yang Jiaming Liao Luyao Feng Sen Wang Zhibin Zhao Jian Wang Yazhong Bu Jian Zhuang De-Wen Zhang Source Type: research