High-resolution separation of DNA/proteins through nanorod sieving matrix
Publication date: Available online 20 April 2019Source: Biosensors and BioelectronicsAuthor(s): Zhen Cao, Yu Zhu, Yang Liu, Shurong Dong, Jiongdong Zhao, Ye Wang, Shu Yang, Junxue FuAbstractIn this paper, we report on an integrated nanorod array as a novel sieving matrix for high-resolution separation of biomolecules. A wide size range of DNA (100 bp–166 kbp) and proteins (11.4 kDa–205 kDa) are resolved into sharp peaks within several minutes using devices with various pore apertures, either in a reptation mode or Ogston mechanism. The device delivers notable performance with the minimum resolvable size diffe...
Source: Biosensors and Bioelectronics - April 22, 2019 Category: Biotechnology Source Type: research

Point-of-care genetic analysis for multiplex pathogenic bacteria on a fully integrated centrifugal microdevice with a large-volume sample
We present a fully integrated portable centrifugal microsystem for multiplex detection of food poisoning bacteria with a large volume of sample up to 1 mL. The microsystem consists of a portable genetic analyzer and a fully integrated centrifugal microdevice. The centrifugal microdevice is designed with two units: a 3D printed solution-loading cartridge and a centrifugal microfluidic disc. All the essential solutions for loop-mediated isothermal amplification (LAMP) reaction are stored inside the cartridge, and orderly released into centrifugal microdevice by a rotation program. Each unit of the device is designed with 2...
Source: Biosensors and Bioelectronics - April 22, 2019 Category: Biotechnology Source Type: research

Vancomycin functionalized WO3 thin film-based impedance sensor for efficient capture and highly selective detection of Gram-positive bacteria
In this study, we report a facile, reusable, and highly sensitive label-free impedance sensor for discriminating Gram-positive and Gram-negative bacteria. The impedance sensor was fabricated using gold interdigitated electrodes onto a tungsten oxide thin film. X-Ray diffraction confirmed the formation of polycrystalline tungsten oxide. Field emission scanning electron microscopy and atomic force microscopy revealed that tungsten oxide has a porous structure. Tungsten oxide was functionalized with vancomycin, a glycopeptide antibiotic known to have a specific interaction with the peptidoglycan layer of Gram-positive bacteri...
Source: Biosensors and Bioelectronics - April 20, 2019 Category: Biotechnology Source Type: research

A microRNA-triggered self-powered DNAzyme walker operating in living cells
Publication date: Available online 19 April 2019Source: Biosensors and BioelectronicsAuthor(s): Chang Liu, Yanlei Hu, Qingshan Pan, Jintao Yi, Juan Zhang, Manman He, Mengyun He, Tingting Chen, Xia ChuAbstractDNA-based nanomachines have received increasing attention due to their great potential to mimic natural biological motors and create novel modes of motion. Here, we report a DNAzyme-based walking machine, which can operate in living cells after triggered by intracellular miRNA-21. The walking machine is constructed by assembling DNAzyme walking strands and FAM-labeled substrate strands on a single gold nanoparticle (Au...
Source: Biosensors and Bioelectronics - April 20, 2019 Category: Biotechnology Source Type: research

Film-Spotting chiral miniPEG-γPNA array for BRCA1 gene mutation detection
Publication date: Available online 19 April 2019Source: Biosensors and BioelectronicsAuthor(s): Bo Dong, Kaixuan Nie, Huanhuan Shi, Lemeng Chao, Mingyang Ma, Fengxiao Gao, Bo Liang, Wei Chen, Mengqiu Long, Zhengchun LiuAbstractPeptide nucleic acids array technology is a method of greatly increasing the throughput of laboratory processes to efficiently perform large-scale genetic tests. Diethylene glycol-containing chiral γPNA (miniPEG-γPNA) is considered to be the best PNA derivative and one of the best candidates for gene detection, because it can hybridize DNA with greater affinity and sequence selectivity th...
Source: Biosensors and Bioelectronics - April 20, 2019 Category: Biotechnology Source Type: research

Flexible bioelectrodes with enhanced wrinkle microstructures for reliable electrochemical modification and neuromodulation in vivo
Publication date: Available online 17 April 2019Source: Biosensors and BioelectronicsAuthor(s): Bowen Ji, Minghao Wang, Chaofan Ge, Zhaoqian Xie, Zhejun Guo, Wen Hong, Xiaowei Gu, Longchun Wang, Zhiran Yi, Chunpeng Jiang, Bin Yang, Xiaolin Wang, Xiuyan Li, Chengyu Li, Jingquan LiuAbstractLimited electrode size with high electrochemical performance and reliability of modified materials are two of the main concerns for flexible neural electrodes in recent years. Here, an effective fabrication method of enhanced micro-scale wrinkles based on oil-pretreated hyperelastic substrates (PDMS and Ecoflex) is proposed for the applica...
Source: Biosensors and Bioelectronics - April 19, 2019 Category: Biotechnology Source Type: research

Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers
Publication date: Available online 18 April 2019Source: Biosensors and BioelectronicsAuthor(s): Yang Wang, Jinping Luo, Juntao Liu, Shuai Sun, Ying Xiong, YuanYuan Ma, Shi Yan, Yue Yang, Huabing Yin, Xinxia CaiAbstractSimultaneous detection of multiple tumor biomarkers in body fluids could facilitate early diagnosis of lung cancer, so as to provide scientific reference for clinical treatment. This paper depicted a multi-parameter paper-based electrochemical aptasensor for simultaneous detection of carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in a clinical sample with high sensitivity and specificity. Th...
Source: Biosensors and Bioelectronics - April 19, 2019 Category: Biotechnology Source Type: research

Ultrabright fluorescent microsphere and its novel application for improving the sensitivity of immunochromatographic assay
Publication date: Available online 17 April 2019Source: Biosensors and BioelectronicsAuthor(s): Gang-gang Zhang, Shao-lan Xu, Yong-hua Xiong, Hong Duan, Wen-yao Chen, Xiang-min Li, Mei-fang Yuan, Wei-hua LaiAbstractFluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then decreased as the c...
Source: Biosensors and Bioelectronics - April 18, 2019 Category: Biotechnology Source Type: research

Highly sensitive and wide-range nanoplasmonic detection of fibrinogen using erythrocyte membrane-blanketed nanoparticles
Publication date: Available online 17 April 2019Source: Biosensors and BioelectronicsAuthor(s): Seongjae Jo, Insu Kim, Wonseok Lee, Minwoo Kim, Joohyung Park, Gyudo Lee, Dae Sung Yoon, Jinsung ParkAbstractFibrinogen, which is a glycoprotein that circulates in the blood, plays various important biological roles, e.g., in blood coagulation, fibroblast proliferation, angiogenesis, and wound healing. Abnormal levels of fibrinogen in plasma have been identified as a key biomarker of a variety of disorders from cardiovascular diseases to hemophilia. Therefore, the development of a quantitative assay for fibrinogen in the blood h...
Source: Biosensors and Bioelectronics - April 18, 2019 Category: Biotechnology Source Type: research

A quencher-free DNAzyme beacon for fluorescently sensing uranyl ions via embedding 2-aminopurine
Publication date: Available online 15 April 2019Source: Biosensors and BioelectronicsAuthor(s): Xiaolong Wang, Rui Zeng, Shengnan Chu, Wei Tang, Na Lin, Jun Fu, Jiangrong Yang, Bo GaoAbstractDNAzyme-based fluorescent probes have provided valuable protocols for detecting uranium, one of the most common radioactive contaminants in the environment, with ultra-high selectivity and sensitivity. Designing novel DNAzyme beacons to update the mode of fluorescence reporting and/or quenching will continuously enhance “turn-on” sensing performance as well as promote actual application of the biological probes. In this wor...
Source: Biosensors and Bioelectronics - April 16, 2019 Category: Biotechnology Source Type: research

Oxygen vacancy enhanced photoelectrochemical performance of Bi2MoO6/B, N co-doped graphene for fabricating lincomycin aptasensor
Publication date: Available online 16 April 2019Source: Biosensors and BioelectronicsAuthor(s): Lan Ge, Qian Liu, Ding Jiang, Lijun Ding, Zuorui Wen, Yingshu Guo, Caifeng Ding, Kun WangAbstractOxygen defect-engineered is an important strategy to improve the photoelectric activity of materials. Herein, a facile one-pot solvothermal method was utilized to synthesize visible light-responsive photoactive Bi2MoO6 nanoparticles anchored boron and nitrogen co-doped graphene (BNG) nanosheets nanocomposites with oxygen vacancy. The incorporation of BNG nanosheets increased the oxygen vacancies amounts on Bi2MoO6 remarkably, and the...
Source: Biosensors and Bioelectronics - April 16, 2019 Category: Biotechnology Source Type: research

Photoelectrochemically driven bioconversion and determination of nifedipine based on a double photoelectrode system
Publication date: Available online 16 April 2019Source: Biosensors and BioelectronicsAuthor(s): Jiuying Tian, Mingjuan Huang, Yanting Yang, Dongyue Wang, Jusheng LuAbstractIn the present work, a double photoelectrode system has been constructed for photoelectrochemically driven enzymatic bioconversion and determination of nifedipine. In which, the TiO2 nanotube arrays in-situ assembled with g-C3N4 (TNA/g-C3N4) was used as a photoanode, and a cytochrome P450 3A4 (CYP3A4) enzyme was immobilized in the porous ITO/CuO films to fabricate an ITO/CuO/CYP3A4 photocathode. The constructed double photoelectrode system had a signific...
Source: Biosensors and Bioelectronics - April 16, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: 1 June 2019Source: Biosensors and Bioelectronics, Volume 134Author(s): (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - April 14, 2019 Category: Biotechnology Source Type: research

Facile one-step fabrication of glucose oxidase loaded polymeric nanoparticles decorating MWCNTs for constructing glucose biosensing platform: Structure matters
Publication date: Available online 13 April 2019Source: Biosensors and BioelectronicsAuthor(s): Sheng Xu, Yuheng Zhang, Ye Zhu, Jie Wu, Kaiyin Li, Geyu Lin, Xiaojie Li, Ren Liu, Xiaoya Liu, Ching-Ping WongAbstractA novel and robust enzymatic biosensing platform with high sensitivity is developed based on facile one-step assembled bio-nanocomposites with enzymes-loaded polymeric nanoparticles decorating multi-walled carbon nanotubes (MWCNTs). An amphiphilic copolymer PAVE containing photo-cross-linkable coumarin segments and carboxylic groups was co-assembled with MWCNTs in aqueous solution while encapsulating the model enz...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification
Publication date: Available online 13 April 2019Source: Biosensors and BioelectronicsAuthor(s): Qing Wang, Liyuan Zou, Xiaohai Yang, Xiaofeng Liu, Wenyan Nie, Yan Zheng, Quan Cheng, Kemin WangAbstractSensitive detection of cancerous exosomes is critical to early diseases diagnosis and prognosis. Herein, a sensitive aptasensor was demonstrated for exosomes detection by surface plasmon resonance (SPR) with dual gold nanoparticle (AuNP)-assisted signal amplification. Dual nanoparticle amplification was achieved by controlled hybridization attachment of AuNPs resulting from electronic coupling between the Au film and AuNPs, as...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Reconfigurable multiplexed point of Care System for monitoring type 1 diabetes patients
Publication date: Available online 13 April 2019Source: Biosensors and BioelectronicsAuthor(s): Augusto Márquez, Joan Aymerich, Michele Dei, Rosalía Rodríguez-Rodríguez, Manuel Vázquez-Carrera, Javier Pizarro-Delgado, Pablo Giménez-Gómez, Ángel Merlos, Lluís Terés, Francesc Serra-Graells, Cecilia Jiménez-Jorquera, Carlos Domínguez, Xavier Muñoz-BerbelAbstractAt the point of care (POC), on-side clinical testing allows fast biomarkers determination even in resource-limited environments. Current POC systems rely on tests selective to a ...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Bimetallic cerium and ferric oxides nanoparticles embedded within mesoporous carbon matrix: Electrochemical immunosensor for sensitive detection of carbohydrate antigen 19-9
Publication date: Available online 11 April 2019Source: Biosensors and BioelectronicsAuthor(s): Minghua Wang, Mengyao Hu, Bin Hu, Chuanpan Guo, Yingpan Song, Qiaojuan Jia, Linghao He, Zhihong Zhang, Shaoming FangAbstractA label-free electrochemical immunosensor was successfully developed for sensitively detecting carbohydrate antigen 19–9 (CA19-9) as a cancer marker. To achieve this, a series of bimetallic cerium and ferric oxide nanoparticles embedded within the mesoporous carbon matrix (represented by CeO2/FeOx@mC) was obtained from the bimetallic CeFe-based metal organic framework (CeFe-MOF) by calcination at diff...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Electron transfer through protein-bound water and its bioelectronic application
This article reports that a metastructure of polypeptides with the bound water can have high and stable electron conductivity without classic electron-conducting components. We used gelatin as the model protein since the peptide chains contain numerous sites capable of forming hydrogen bonds with water molecules. The lack of redox sites and the trace amounts of aromatic amino acids also eliminate the possibility that the electron transfer is due to redox reactions or pi-stacking. Our Raman spectroscopy results show that the high electron-conductive metastructure is composed of bound water and unwound gelatin polypeptides. ...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Bio-Electron-Fenton (BEF) process driven by sediment microbial fuel cells (SMFCs) for antibiotics desorption and degradation
Publication date: Available online 11 April 2019Source: Biosensors and BioelectronicsAuthor(s): Yuezhu Wang, Hanmin Zhang, Yujie Feng, Baikun Li, Mingchuan Yu, Xiaotong Xu, Lu CaiAbstractA sediment microbial fuel cell electro-Fenton (SMFC-E-Fenton) system was proposed in this study by utilizing the biological electrons produced from an SMFC to power an E-Fenton process. Antibiotics with different absorbability in both aqueous and solid phase can be removed by this system at room temperature and pressure condition, without external power or other chemical reagents. γ-FeOOH graphene polyacrylamide carbonized aerogel (&...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Permselective glucose sensing with GLUT1-Rich cancer cell membranes
Publication date: Available online 12 April 2019Source: Biosensors and BioelectronicsAuthor(s): Insu Kim, Dohyung Kwon, Dongtak Lee, Gyudo Lee, Dae Sung YoonAbstractEnzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivit...
Source: Biosensors and Bioelectronics - April 13, 2019 Category: Biotechnology Source Type: research

Synthesis of wearable and flexible NiP0.1-SnOx/PANI/CuO/cotton towards a non-enzymatic glucose sensor
Publication date: Available online 10 April 2019Source: Biosensors and BioelectronicsAuthor(s): Ali Sedighi, Majid Montazer, Saeedeh MazinaniAbstractNi-SnOx, PANI and CuO nanoparticles were synthesized on cotton fabric through chemical methods to make a new flexible high-performance non-enzymatic glucose sensor. FESEM, XRD, XPS, EDS and ATR analysis were employed to characterize the structure and the morphology of the nanomaterials. The high electrochemical performance of nickel and copper oxide and hydroxide on a conductive template leads to fabrication of a wearable and flexible cotton electrode with an excellent electro...
Source: Biosensors and Bioelectronics - April 11, 2019 Category: Biotechnology Source Type: research

Electrochemical simultaneous analysis of dopamine and epinephrine using double imprinted One MoNomer acryloylated graphene oxide-carbon black composite polymer
Publication date: Available online 11 April 2019Source: Biosensors and BioelectronicsAuthor(s): Sana Fatma, Bhim Bali Prasad, Swadha Jaiswal, Richa Singh, Kislay SinghAbstractA novel One MoNomer dual imprinted graphene oxide/carbon black composite polymer was developed applying ‘surface-grafting from’ approach on the screen printed carbon electrode for the electrochemical sensing of dopamine and epinephrine. Acryloylated-graphene oxide/carbon black was synthesized for the first time. This served both as a crosslinker and monomer leading to the fast electron transfer from the redox centre to the electrode. The o...
Source: Biosensors and Bioelectronics - April 11, 2019 Category: Biotechnology Source Type: research

Automated DNA hybridization transfer with movable super-paramagnetic microbeads in a microflow reactor
Publication date: Available online 11 April 2019Source: Biosensors and BioelectronicsAuthor(s): Robert PenchovskyAbstractAn automated DNA hybridization transfer in a microflow reactor is demonstrated by moving paramagnetic beads between two spatially separate solutions with different pH values. The microbeads-based microfluidic platform is fully automated and programmable. It employs a robust chemical procedure for specific DNA hybridization transfer in microfluidic devices under isothermal conditions based on reversible pH alterations. The method takes advantage of high-speed DNA hybridization and denaturation on beads un...
Source: Biosensors and Bioelectronics - April 11, 2019 Category: Biotechnology Source Type: research

Fully integrated and slidable paper-embedded plastic microdevice for point-of-care testing of multiple foodborne pathogens
This study presents a slidable paper-embedded plastic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities. The developed microdevice consists of three layers that allow a sliding movement to mix the sample and reagents for DNA purification, amplification, and detection in a sequential manner. An FTA card was employed in the main chamber for DNA extraction and purification from intact bacterial cells. Subsequently, LAMP reagents and fuchsin-stored chambers were pulled toward the main chambers for DNA amplifications at 65 °C. After ...
Source: Biosensors and Bioelectronics - April 11, 2019 Category: Biotechnology Source Type: research

A ratiometric fluorescence platform based on boric-acid-functional Eu-MOF for sensitive detection of H2O2 and glucose
Publication date: Available online 10 April 2019Source: Biosensors and BioelectronicsAuthor(s): Yu Cui, Fei Chen, Xue-Bo YinAbstractA Eu-metal organic framework (MOF) probe with dual-emission was reported for the ratiometric fluorescence detection of H2O2 and glucose. Because of the special nucleophilic reaction between boric group and H2O2, Eu3+ and 5-boronobenzene-1,3-dicarboxylic acid (BBDC) were selected to synthesize the functional MOF probe via a simple one-pot solvothermal method. The Eu-MOF shows dual-emission at 370 and 623 nm with the single excitation at 270 nm due to the energy transfer efficiency change fo...
Source: Biosensors and Bioelectronics - April 10, 2019 Category: Biotechnology Source Type: research

Enzyme-free glucose sensor based on layer-by-layer electrodeposition of multilayer films of multi-walled carbon nanotubes and Cu-based metal framework modified glassy carbon electrode
Publication date: Available online 9 April 2019Source: Biosensors and BioelectronicsAuthor(s): Lan Wu, Zhiwei Lu, Jianshan YeAbstractA high-performance nonenzymatic glucose sensor was successfully prepared by a layer by layer strategy through electrodeposition assembling multilayer films of Cu-metal-organic frameworks/multi-walled carbon nanotubes (Cu-MOF/MWNTs) modified glassy carbon electrodes (GCE). Different multilayer films of Cu-MOF/MWNTs modified GCE (Cu-MOF/MWNTs/GCE) were prepared by repeating the electrodeposition of MWNTs onto the GCE in an MWNTs solution (MWNTs/GCE) and electrodeposition of the Cu-MOF layer ont...
Source: Biosensors and Bioelectronics - April 10, 2019 Category: Biotechnology Source Type: research

Phosphorene-gold nanocomposite based microfluidic aptasensor for the detection of okadaic acid
This study demonstrates the preparation and functioning of an electrochemical microfluidic biochip for the detection of OA. The screen printed carbon electrode (SPCE) was modified by phosphorene-gold nanocomposite onto which an aptamer specific to OA was immobilized. BP-Au nanocomposites were synthesized by in-situ, on-step method without the use of a reducing agent. Potassium ferro-ferri cyanide was used as a redox pair to quantify signal strength. To improve reaction time, increase sensitivity and portability, a microfluidic platform was designed and developed. This device comprised of channels identified for specific pu...
Source: Biosensors and Bioelectronics - April 7, 2019 Category: Biotechnology Source Type: research

An ultrasensitive electrochemiluminescence biosensor for MicroRNA detection based on luminol-functionalized Au NPs@ZnO nanomaterials as signal probe and dissolved O2 as coreactant
Publication date: Available online 6 April 2019Source: Biosensors and BioelectronicsAuthor(s): Xiaoli Zhang, Weimin Li, Ying Zhou, Yaqin Chai, Ruo YuanAbstractIn this work, the ZnO nanostars with excellent catalytic performance were firstly used as the coreaction accelerator of luminol-O2 system to construct a biosensor for ultrasensitively detecting microRNA-21 (miRNA-21) in cancer cells. Specifically, ZnO nanostars could expedite the reduction of dissolved O2, generating more reactive oxygen species (ROSs) to extremely promote electrochemiluminescence (ECL) luminous efficiency of luminol. Thus luminol-functionalized Au N...
Source: Biosensors and Bioelectronics - April 7, 2019 Category: Biotechnology Source Type: research

Application of conducting polymers to wound care and skin tissue engineering: A review
Publication date: Available online 6 April 2019Source: Biosensors and BioelectronicsAuthor(s): Milena Talikowska, Xiaoxu Fu, Grzegorz LisakAbstractThe use of intrinsically conducting polymers (CPs) in wound care and skin tissue engineering presents a novel opportunity for accelerated wound healing, enhanced antibacterial activity and the potential for controlled drug delivery. Through their increased electrical conductivity, CPs can facilitate the application of electrical stimulation directly to the wound area, which has been linked to faster wound healing. The release of drugs or biological agents to the wound site can l...
Source: Biosensors and Bioelectronics - April 7, 2019 Category: Biotechnology Source Type: research

Hydrodynamic cavitation for the rapid separation and electrochemical detection of Cryptosporidium parvum and Escherichia coli O157:H7 in ground beef
Publication date: Available online 6 April 2019Source: Biosensors and BioelectronicsAuthor(s): Ugochukwu C. Nze, Michael G. Beeman, Christopher J. Lambert, Ghadhanfer Salih, Bruce K. Gale, Himanshu J. SantAbstract:Foodborne illnesses are a major contributor to misery and health challenges in both rich and poor nations. Illnesses from pathogens such as Escherichia coli and Cryptosporidium parvum oocysts account for most of the cases of diarrhea in the world. Many standard methods exist for detecting these pathogens in water. However, these standard methods do not readily translate to the detection of the same pathogens in f...
Source: Biosensors and Bioelectronics - April 7, 2019 Category: Biotechnology Source Type: research

Fabrication of hierarchical MIL-68(In)-NH2/MWCNT/CdS composites for constructing label-free photoelectrochemical tetracycline aptasensor platform
Publication date: Available online 4 April 2019Source: Biosensors and BioelectronicsAuthor(s): Xue Zhang, Tao Yan, Tingting Wu, Yixuan Feng, Meng Sun, Liangguo Yan, Bin Du, Qin WeiAbstractIt is essential to develop a highly efficient detection platform for tetracycline (Tc). Herein, based on well-designed hierarchical MIL-68(In)-NH2/MWCNT/CdS composites as a highly efficient transducer, a label-free visible light driven photoelectrochemical (PEC) aptasensor was systematically fabricated. Characterization results indicate that the forming of MIL-68(In)-NH2/CdS heterojunction remarkable facilitated the transfer and inhibited...
Source: Biosensors and Bioelectronics - April 5, 2019 Category: Biotechnology Source Type: research

Direct antimicrobial susceptibility testing of bloodstream infection on SlipChip
Publication date: Available online 4 April 2019Source: Biosensors and BioelectronicsAuthor(s): Qiaolian Yi, Dongyang Cai, Meng Xiao, Mengyue Nie, Qinna Cui, Jingwei Cheng, Caiming Li, Jie Feng, Gerald Urban, Ying-Chun Xu, Ying Lan, Wenbin DuAbstractThis paper describes an integrated microfluidic SlipChip device for rapid antimicrobial susceptibility testing (AST) of bloodstream pathogens in positive blood cultures. Unlike conventional AST methods, which rely on an overnight subculture of positive blood cultures to obtain isolated colonies, this device enables direct extraction and enrichment of the bacteria from positive b...
Source: Biosensors and Bioelectronics - April 5, 2019 Category: Biotechnology Source Type: research

Sampling and multiplexing in lab-on-paper bioelectroanalytical devices for glucose determination
Publication date: Available online 5 April 2019Source: Biosensors and BioelectronicsAuthor(s): O. Amor-Gutiérrez, E. Costa-Rama, M.T. Fernández-AbedulAbstractIn this work, we present a multiplexed (eight simultaneous measurements) paper-based electrochemical device developed in a very simple way and using low-cost materials, such as paper, carbon ink and multifunctional connector headers. Meanwhile, we have also combined the paper-based electrochemical platform with a glass-fiber strip in order to integrate easily a sampling step. Both approaches, simultaneous measuring and sampling, have been applied to the ...
Source: Biosensors and Bioelectronics - April 5, 2019 Category: Biotechnology Source Type: research

A paper-based electrochemical immunosensor with reduced graphene oxide/thionine/gold nanoparticles nanocomposites modification for the detection of cancer antigen 125
Publication date: Available online 5 April 2019Source: Biosensors and BioelectronicsAuthor(s): Yan Fan, Shengyu Shi, Junshuang Ma, Yaohua GuoAbstractA paper-based electrochemical immunosensor was developed for the detection of cancer antigen 125 (CA125) by screen-printing technique. The reduced graphene oxide/thionine/gold nanoparticles (rGO/Thi/AuNPs) nanocomposites were compounded and coated onto the working electrode of immunosensor for CA125 antibody (anti-CA125) immobilization and detection signal amplification. The detection principle was based on the fact that the immunocomplex formed by specify binding of CA125 ant...
Source: Biosensors and Bioelectronics - April 5, 2019 Category: Biotechnology Source Type: research

An extended core nanocoax pillar architecture for enhanced molecular detection
Publication date: 1 June 2019Source: Biosensors and Bioelectronics, Volume 134Author(s): L.A. D’Imperio, A.E. Valera, J.R. Naughton, M.M. Archibald, J.M. Merlo, T.J. Connolly, M.J. Burns, T.C. Chiles, M.J. NaughtonAbstractBiosensors that incorporate nanomaterials and nanofabrication techniques enable molecular detection of chemical and biological macromolecules with a high degree of specificity and ultrasensitivity. Here, we present a novel fabrication process that yields a nanostructure capable of detecting biological macromolecules. The extended core nanocoax (ECC) structure builds on a previously reported nanocoax...
Source: Biosensors and Bioelectronics - April 5, 2019 Category: Biotechnology Source Type: research

Dual-signal-amplified electrochemiluminescence biosensor for microRNA detection by coupling cyclic enzyme with CdTe QDs aggregate as luminophor
Publication date: Available online 3 April 2019Source: Biosensors and BioelectronicsAuthor(s): Hong-Yun Zhu, Shou-Nian DingAbstractIn this work, a dual-signal-amplified electrochemiluminescence (ECL) biosensor was proposed for the first time to detect microRNAs (miRNAs) based on cyclic enzyme and seeded-watermelon-like mesoporous nanospheres (mSiO2@CdTe@SiO2, mSQS NSs). mSQS NSs were successfully fabricated by inlaying the CdTe quantum dots (QDs) into the mesoporous silica (mSiO2) and future coating the surface with the silica layer. The obtained mSQS NSs contained tens of QDs and exhibited much stronger ECL signal than si...
Source: Biosensors and Bioelectronics - April 4, 2019 Category: Biotechnology Source Type: research

Specific detection of avian influenza H5N2 whole virus particles on lateral flow strips using a pair of sandwich-type aptamers
This study shows the promising perspective of a cognate pair of aptamers for the on-site detection system which could be useful for rapid detection of avian influenza viruses for preventing the pandemic influenza viruses from spreading. (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - April 2, 2019 Category: Biotechnology Source Type: research

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP
Publication date: Available online 30 March 2019Source: Biosensors and BioelectronicsAuthor(s): Ivan Magriñá, Anna Toldrà, Mònica Campàs, Mayreli Ortiz, Anna Simonova, Ioanis Katakis, Michal Hocek, Ciara K. O'SullivanAbstractAn electrochemical genosensor for the detection and quantification of Karlodinium armiger is presented. The genosensor exploits tailed primers and ferrocene labelled dATP analogue to produce PCR products that can be directly hybridised on a gold electrode array and quantitatively measured using square wave voltammetry. Tailed primers consist of a sequence specific for...
Source: Biosensors and Bioelectronics - March 31, 2019 Category: Biotechnology Source Type: research

Binding-induced formation of DNAzyme on an Au@Ag nanoparticles/TiO2 nanorods electrode: Stimulating biocatalytic precipitation amplification for plasmonic photoelectrochemical bioanalysis
Publication date: Available online 30 March 2019Source: Biosensors and BioelectronicsAuthor(s): Ling Zhang, Xiao-Mei Shi, Yi-Tong Xu, Gao-Chao Fan, Xiao-Dong Yu, Yan-Yu Liang, Wei-Wei ZhaoAbstractPhotoelectrochemical (PEC) DNA bioanalysis has been drawing more attention in recent years due to the advantages of PEC technique and the vital importance of DNA biomolecules. DNAzymes are unique catalytic nucleic acid molecules that are capable of catalyzing specific biochemical reactions. Using the target-binding-induced conformation change of hairpin DNA probe to hemin/G-quadruplex-based DNAzyme and a plasmonic Au@Ag nanopartic...
Source: Biosensors and Bioelectronics - March 31, 2019 Category: Biotechnology Source Type: research

A dual-response biosensor for electrochemical and glucometer detection of DNA methyltransferase activity based on functionalized metal-organic framework amplification
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Ying Chen, Xian-Zhu Meng, Hui-Wen Gu, Hong-Chao Yi, Wei-Yin SunAbstractDNA methylation is catalyzed by DNA methyltransferase (MTase) and concerned with many biological processes including pathogenesis of various human diseases. The monitoring of MTase activity is thus of great significance in disease diagnosis and drug screening. Herein, we developed a facile way to synthesize biocompatible invertase enzyme modified metal-organic framework (Invertase/MOF) materials, and explored its application in constructing a dual-response Da...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

Silver-based metal-organic gels as novel coreactant for enhancing electrochemiluminescence and its biosensing potential
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Yang Li, Li He, Cheng Zhi Huang, Yuan Fang LiAbstractMetal-organic gels (MOGs), a kind of soft materials, were reported as a brand new category of novel solid coreactants for enhancing electrochemiluminescence (ECL) of Ru (bpy)32+ and derivatives. In this work, silver-based MOGs (Ag-MOGs) prepared by a one-step mixing and heating approach were demonstrated as novel efficient solid-state coreactants which differ from the previous research on ECL coreactant nanomaterials. It was discussed that the possible enhancement mechanism wa...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

Graphene-based fully integrated portable nanosensing system for on-line detection of cytokine biomarkers in saliva
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Zhuang Hao, Yunlu Pan, Wenwen Shao, Qiao Lin, Xuezeng ZhaoAbstractSaliva has been reported to contain various cytokine biomarkers which are associated with some severe diseases such as cancers. Non-invasive saliva diagnosis using wearable or portable devices may pave a new avenue for monitoring conditions of the high risk population. Here, a graphene-based fully integrated portable nanosensing system, the entire size of which is smaller than a smart-phone and can be handheld, is presented for on-line detection of cytokine biomar...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

A review of microfabricated electrochemical biosensors for DNA detection
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Ewen O. Blair, Damion K. CorriganAbstractThis review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

A portable nucleic acid detection system using natural convection combined with a smartphone
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Vinoth Kumar Rajendran, Padmavathy Bakthavathsalam, Peter L. Bergquist, Anwar SunnaAbstractThe development of portable nucleic acid diagnostic devices has the potential to expand the availability of molecular diagnostics into low-resource settings. One of the promising solutions for rapid and simple DNA amplification is the use of Rayleigh–Bernard natural convection which is caused by a buoyancy-driven thermal gradient of liquid when heated from below. This natural convection avoids the use of the complex and sophisticated...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

Molecularly imprinted polymer-decorated signal on-off ratiometric electrochemical sensor for selective and robust dopamine detection
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Jiao Yang, Yue Hu, Yingchun LiAbstractDopamine (DA), as one of the central neurotransmitters, plays an important role in many physiological and pathological processes. Detection of DA is critical to diagnose and monitor some neurological diseases. In this work, a novel on-off ratiometric electrochemical sensor with molecularly imprinted polymers (MIPs) as target molecule recognizer has been developed for selective and accurate detection of DA. Nanoporous gold (NPG) was electrodeposited on bare gold electrode, which not only bene...
Source: Biosensors and Bioelectronics - March 30, 2019 Category: Biotechnology Source Type: research

Simple “signal-on” photoelectrochemical aptasensor for ultrasensitive detecting AFB1 based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au nanocomposites
Publication date: Available online 28 March 2019Source: Biosensors and BioelectronicsAuthor(s): Bin Zhang, Yan Lu, Chaonan Yang, Qingfu Guo, Guangming NieAbstractA simple “signal-on” photoelectrochemical (PEC) aptasensor is constructed for Aflatoxin B1 (AFB1) detection based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au (erGO/P5FIn/Au) nanocomposites. The nanocomposites are synthesized by simple electrochemical deposition method and show good photoelectrochemical performance. Poly(5-formylindole) (P5FIn) can generate electron-hole pairs under light irradiation, leading to the formation of ...
Source: Biosensors and Bioelectronics - March 29, 2019 Category: Biotechnology Source Type: research

Glucose oxidase mimicking half–sandwich nickel(II) complexes of coumarin substituted N–heterocyclic carbenes as novel molecular electrocatalysts for ultrasensitive and selective determination of glucose
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): K.N. Brinda, Gautam Achar, Jan Grzegorz Małecki, Srinivasa Budagumpi, D.H. Nagaraju, V. Suvina, R. Geetha BalakrishnaAbstractGlucose oxidase mimicking nickel–based porous structures with organic anchors are developed as cheap and reliable electrochemical sensors for the quantitative detection of glucose. A series of sterically and electronically modulated, air– and moisture–stable half–sandwich nickel(II) NHC complexes were prepared and characterized. Under the optimized electrocatalytic conditions, the ...
Source: Biosensors and Bioelectronics - March 29, 2019 Category: Biotechnology Source Type: research

Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA nanotetrahedron structure
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Duanping Sun, Zibin Luo, Jing Lu, Shangshi Zhang, Tong Che, Zuanguang Chen, Luyong ZhangAbstractThe accurate detection of biomarkers for acute myocardial infarction (AMI) plays an important role in clinical diagnosis and management process. In this work, we developed an electrochemical biosensor by using magnetic metal organic framework (MMOF) nanocatalysts and DNA nanotetrahedron (NTH) based dual-aptamer probes for nonenzymatic detection of cardiac troponin I (cTnI), a gold standard biomarker for the early diagnosis of AMI. Fir...
Source: Biosensors and Bioelectronics - March 29, 2019 Category: Biotechnology Source Type: research

All-solid-state metal-mediated Z-scheme photoelectrochemical immunoassay with enhanced photoexcited charge-separation for monitoring of prostate-specific antigen
Publication date: Available online 29 March 2019Source: Biosensors and BioelectronicsAuthor(s): Lijia Zhang, Zhongbin Luo, Ruijin Zeng, Qian Zhou, Dianping TangAbstractAn all-solid-state metal-mediated Z-scheme photoelectrochemical (PEC) immunoassay was designed for sensitive detection of prostate-specific antigen (PSA) by using WO3-Au-CdS nanocomposite as photoactive material and copper ion (Cu2+) as an inhibitor. The Z-scheme PEC system comprising of CdS nanoparticle (photosystem I; PS I), WO3 nanorod (photosystem II; PS II) and gold nanoparticle (Au NP; solid electron mediator) was reasonably established by a simple and...
Source: Biosensors and Bioelectronics - March 29, 2019 Category: Biotechnology Source Type: research

Novel strategy to improve the sensing performances of split ATP aptamer based fluorescent indicator displacement assay through enhanced molecular recognition
Publication date: Available online 27 March 2019Source: Biosensors and BioelectronicsAuthor(s): Yu Ma, Fenghua Geng, Yongxiang Wang, Maotian Xu, Congying Shao, Peng Qu, Yintang Zhang, Baoxian YeAbstractSplit aptamer strategy was often used to improve the sensitivity of aptasensor. However, traditional split aptamer strategy can not be directly used to improve the label-free aptamer based Thioflavin T (ThT) displacement assay for ATP because the split ATP aptamer display much lower enhancement effects on the fluorescence of ThT than intact aptamer. In order to address this issue, this is the first report using G-rich DNA se...
Source: Biosensors and Bioelectronics - March 28, 2019 Category: Biotechnology Source Type: research