A novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension reaction
Biosens Bioelectron. 2024 Feb 29;253:116174. doi: 10.1016/j.bios.2024.116174. Online ahead of print.ABSTRACTWe herein present a novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP) reaction. The detection probe employed as a key component in this technique serves as a substrate for RNase H and triggers the PS-THSP reaction upon the RNase H-mediated degradation of the probe. As a consequence, a large number of long concatemeric amplification products could be produced and used to identify the RNase H activity through the fluorescence signals produced b...
Source: Biosensors and Bioelectronics - March 3, 2024 Category: Biotechnology Authors: Junhyeok Yoon Jinhwan Lee Jaemin Kim Sang Mo Lee Soohyun Kim Hyun Gyu Park Source Type: research
In situ self-referenced intracellular two-electrode system for enhanced accuracy in single-cell analysis
Biosens Bioelectron. 2024 Feb 28;253:116173. doi: 10.1016/j.bios.2024.116173. Online ahead of print.ABSTRACTSince the emergence of single-cell electroanalysis, the two-electrode system has become the predominant electrochemical system for real-time behavioral analysis of single-cell and multicellular populations. However, due to the transmembrane placement of the two electrodes, cellular activities can be interrupted by the transmembrane potentials, and the test results are susceptible to influences from factors such as intracellular solution, membrane, and bulk solution. These limitations impede the advancement of single-...
Source: Biosensors and Bioelectronics - March 3, 2024 Category: Biotechnology Authors: Qingda Xu Ye Xi Longchun Wang Mengfei Xu Tao Ruan Zhiyuan Du Chunpeng Jiang Jiawei Cao Xiantao Zhu Xiaolin Wang Bin Yang Jingquan Liu Source Type: research
A novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension reaction
Biosens Bioelectron. 2024 Feb 29;253:116174. doi: 10.1016/j.bios.2024.116174. Online ahead of print.ABSTRACTWe herein present a novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP) reaction. The detection probe employed as a key component in this technique serves as a substrate for RNase H and triggers the PS-THSP reaction upon the RNase H-mediated degradation of the probe. As a consequence, a large number of long concatemeric amplification products could be produced and used to identify the RNase H activity through the fluorescence signals produced b...
Source: Biosensors and Bioelectronics - March 3, 2024 Category: Biotechnology Authors: Junhyeok Yoon Jinhwan Lee Jaemin Kim Sang Mo Lee Soohyun Kim Hyun Gyu Park Source Type: research
In situ self-referenced intracellular two-electrode system for enhanced accuracy in single-cell analysis
Biosens Bioelectron. 2024 Feb 28;253:116173. doi: 10.1016/j.bios.2024.116173. Online ahead of print.ABSTRACTSince the emergence of single-cell electroanalysis, the two-electrode system has become the predominant electrochemical system for real-time behavioral analysis of single-cell and multicellular populations. However, due to the transmembrane placement of the two electrodes, cellular activities can be interrupted by the transmembrane potentials, and the test results are susceptible to influences from factors such as intracellular solution, membrane, and bulk solution. These limitations impede the advancement of single-...
Source: Biosensors and Bioelectronics - March 3, 2024 Category: Biotechnology Authors: Qingda Xu Ye Xi Longchun Wang Mengfei Xu Tao Ruan Zhiyuan Du Chunpeng Jiang Jiawei Cao Xiantao Zhu Xiaolin Wang Bin Yang Jingquan Liu Source Type: research
A skin-interfaced, miniaturized platform for triggered induction, capture and colorimetric multicomponent analysis of microliter volumes of sweat
Biosens Bioelectron. 2024 Feb 23;253:116166. doi: 10.1016/j.bios.2024.116166. Online ahead of print.ABSTRACTEccrine sweat can serve as a source of biomarkers for assessing physiological health and nutritional balance, for tracking loss of essential species from the body and for evaluating exposure to hazardous substances. The growing interest in this relatively underexplored class of biofluid arises in part from its non-invasive ability for capture and analysis. The simplest devices, and the only ones that are commercially available, exploit soft microfluidic constructs and colorimetric assays with purely passive modes of ...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Joohee Kim Seyong Oh Da Som Yang Larissa Rugg Radhika Mathur Sung Soo Kwak Seonggwang Yoo Shupeng Li Evangelos E Kanatzidis Geumbee Lee Hong-Joon Yoon Yonggang Huang Roozbeh Ghaffari Susanna A McColley John A Rogers Source Type: research
An automatic glucose monitoring system based on periplasmic binding proteins for online bioprocess monitoring
Biosens Bioelectron. 2024 Feb 23;253:116138. doi: 10.1016/j.bios.2024.116138. Online ahead of print.ABSTRACTGlucose is one of the most vital nutrients in all living organisms, so its monitoring is critical in healthcare and bioprocessing. Enzymatic sensors are more popular as a technology solution to meet the requirement. However, periplasmic binding proteins have been investigated extensively for their high sensitivity, enabling microdialysis sampling to replace existing complex and expensive glucose monitoring solutions based on enzymatic sensors. The binding proteins are used as optical biosensors by introducing an envi...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Hasibul Hasan Vikash Kumar Xudong Ge Chad Sundberg Christopher Slaughter Govind Rao Source Type: research
Label-free single-cell analysis in microdroplets using a light-scattering-based optofluidic chip
Biosens Bioelectron. 2024 Feb 20;253:116148. doi: 10.1016/j.bios.2024.116148. Online ahead of print.ABSTRACTDroplet-based single-cell analysis is a very powerful tool for studying phenotypic and genomic heterogeneity at single-cell resolution for a variety of biological problems. In conventional two-phase droplet microfluidics, due to the mismatch in optical properties between oil and aqueous phases, light scattering mainly happens at the oil/water interface that disables light-scattering-based cell analysis confined in microdroplets. Detection and analysis of cells in microdroplets thus mostly rely on the fluorescence lab...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Li Liang Minhui Liang Zewen Zuo Ye Ai Source Type: research
A personal glucose meter-utilized strategy for portable and label-free detection of hydrogen peroxide
Biosens Bioelectron. 2024 Feb 19;253:116141. doi: 10.1016/j.bios.2024.116141. Online ahead of print.ABSTRACTRapid and precise detection of hydrogen peroxide (H2O2) holds great significance since it is linked to numerous physiological and inorganic catalytic processes. We herein developed a label-free and washing-free strategy to detect H2O2 by employing a hand-held personal glucose meter (PGM) as a signal readout device. By focusing on the fact that the reduced redox mediator ([Fe(CN)6]4-) itself is responsible for the final PGM signal, we developed a new PGM-based strategy to detect H2O2 by utilizing the target H2O2-media...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Sangmo Lee Hyoyong Kim Junhyeok Yoon Yong Ju Hyun Gyu Park Source Type: research
A skin-interfaced, miniaturized platform for triggered induction, capture and colorimetric multicomponent analysis of microliter volumes of sweat
Biosens Bioelectron. 2024 Feb 23;253:116166. doi: 10.1016/j.bios.2024.116166. Online ahead of print.ABSTRACTEccrine sweat can serve as a source of biomarkers for assessing physiological health and nutritional balance, for tracking loss of essential species from the body and for evaluating exposure to hazardous substances. The growing interest in this relatively underexplored class of biofluid arises in part from its non-invasive ability for capture and analysis. The simplest devices, and the only ones that are commercially available, exploit soft microfluidic constructs and colorimetric assays with purely passive modes of ...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Joohee Kim Seyong Oh Da Som Yang Larissa Rugg Radhika Mathur Sung Soo Kwak Seonggwang Yoo Shupeng Li Evangelos E Kanatzidis Geumbee Lee Hong-Joon Yoon Yonggang Huang Roozbeh Ghaffari Susanna A McColley John A Rogers Source Type: research
An automatic glucose monitoring system based on periplasmic binding proteins for online bioprocess monitoring
Biosens Bioelectron. 2024 Feb 23;253:116138. doi: 10.1016/j.bios.2024.116138. Online ahead of print.ABSTRACTGlucose is one of the most vital nutrients in all living organisms, so its monitoring is critical in healthcare and bioprocessing. Enzymatic sensors are more popular as a technology solution to meet the requirement. However, periplasmic binding proteins have been investigated extensively for their high sensitivity, enabling microdialysis sampling to replace existing complex and expensive glucose monitoring solutions based on enzymatic sensors. The binding proteins are used as optical biosensors by introducing an envi...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Hasibul Hasan Vikash Kumar Xudong Ge Chad Sundberg Christopher Slaughter Govind Rao Source Type: research
Label-free single-cell analysis in microdroplets using a light-scattering-based optofluidic chip
Biosens Bioelectron. 2024 Feb 20;253:116148. doi: 10.1016/j.bios.2024.116148. Online ahead of print.ABSTRACTDroplet-based single-cell analysis is a very powerful tool for studying phenotypic and genomic heterogeneity at single-cell resolution for a variety of biological problems. In conventional two-phase droplet microfluidics, due to the mismatch in optical properties between oil and aqueous phases, light scattering mainly happens at the oil/water interface that disables light-scattering-based cell analysis confined in microdroplets. Detection and analysis of cells in microdroplets thus mostly rely on the fluorescence lab...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Li Liang Minhui Liang Zewen Zuo Ye Ai Source Type: research
A personal glucose meter-utilized strategy for portable and label-free detection of hydrogen peroxide
Biosens Bioelectron. 2024 Feb 19;253:116141. doi: 10.1016/j.bios.2024.116141. Online ahead of print.ABSTRACTRapid and precise detection of hydrogen peroxide (H2O2) holds great significance since it is linked to numerous physiological and inorganic catalytic processes. We herein developed a label-free and washing-free strategy to detect H2O2 by employing a hand-held personal glucose meter (PGM) as a signal readout device. By focusing on the fact that the reduced redox mediator ([Fe(CN)6]4-) itself is responsible for the final PGM signal, we developed a new PGM-based strategy to detect H2O2 by utilizing the target H2O2-media...
Source: Biosensors and Bioelectronics - March 1, 2024 Category: Biotechnology Authors: Sangmo Lee Hyoyong Kim Junhyeok Yoon Yong Ju Hyun Gyu Park Source Type: research
AIEgen-deep: Deep learning of single AIEgen-imaging pattern for cancer cell discrimination and preclinical diagnosis
This study introduces AIEgen-Deep, an innovative classification program combining AIEgen fluorescent dyes, deep learning algorithms, and the Segment Anything Model (SAM) for accurate cancer cell identification. Our approach significantly reduces manual annotation efforts by 80%-90%. AIEgen-Deep demonstrates remarkable accuracy in recognizing cancer cell morphology, achieving a 75.9% accuracy rate across 26,693 images of eight different cell types. In binary classifications of healthy versus cancerous cells, it shows enhanced performance with an accuracy of 88.3% and a recall rate of 79.9%. The model effectively distinguish...
Source: Biosensors and Bioelectronics - February 29, 2024 Category: Biotechnology Authors: Haojun Hua Yanlin Deng Jing Zhang Xiang Zhou Tianfu Zhang Bee Luan Khoo Source Type: research
Fibroblast activation protein-sensitive polymeric nanobeacon for early diagnosis of renal fibrosis
Biosens Bioelectron. 2024 Feb 24;253:116144. doi: 10.1016/j.bios.2024.116144. Online ahead of print.ABSTRACTEarly diagnosis and treatment of renal fibrosis (RF) significantly affect the clinical outcomes of chronic kidney diseases (CKDs). As the typical fibrotic ailment, RF is characterized by remodeling of the extracellular matrix, and the activation of fibroblast activation protein (FAP) plays a crucial role in the mediation of extracellular matrix protein degradation. Therefore, FAP can serve as a biomarker for RF. However, up to now, no effective tools have been reported to diagnose early-stage RF via detecting FAP. In...
Source: Biosensors and Bioelectronics - February 29, 2024 Category: Biotechnology Authors: Ni Zeng Xiuhong Guan Xiaowen Liu Huiqiu Shi Nan Li Ruimeng Yang Yibo Zhou Source Type: research
A highly integrated digital PCR system with on-chip heating for accurate DNA quantitative analysis
Biosens Bioelectron. 2024 Feb 23;253:116167. doi: 10.1016/j.bios.2024.116167. Online ahead of print.ABSTRACTDigital polymerase chain reaction (dPCR) is extensively used for highly sensitive disease diagnosis due to its single-molecule detection ability. However, current dPCR systems require intricate DNA sample distribution, rely on cumbersome external heaters, and exhibit sluggish thermal cycling, hampering efficiency and speed of the dPCR process. Herein, we presented the development of a microwell array based dPCR system featuring an integrated self-heating dPCR chip. By utilizing hydrodynamic and electrothermal simulat...
Source: Biosensors and Bioelectronics - February 29, 2024 Category: Biotechnology Authors: Kang Peng Zhihong Wu Zhongxin Feng Ruijun Deng Xiangguo Ma Beiyuan Fan Haonan Liu Zhuzhu Tang Zijian Zhao Yanzhao Li Source Type: research
